CN112175888B - Bacillus belgii Hsg1949 and application thereof - Google Patents
Bacillus belgii Hsg1949 and application thereof Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
Abstract
The invention discloses a Bacillus belgii strainBacillus velezensis) Hsg1949 with preservation number of CGMCC No. 20836. The bacteriostasis rate of the Bacillus beleisi Hsg1949 to the peanut fruit rot reaches 58.7-72.9%, and the field control effect of the Bacillus beleisi Hsg1949 to the peanut fruit rot can reach 71.24%. The bacillus beilesensis can be prepared into corresponding microbial agents to be applied to peanut fields, and can effectively prevent peanut rot. This is achieved byIn addition, the Bacillus beleisi Hsg1949 has a wide antibacterial spectrum, and has certain inhibitory effects on rhizoctonia solani, verticillium wilt and rhizoctonia solani besides the peanut fruit rot.
Description
Technical Field
The invention relates to the field of biological control, in particular to Bacillus belgii Hsg1949 and application of the Bacillus belgii to control of plant diseases such as peanut rot and the like.
Background
The peanut rot is an important disease in peanut production, the main disease of the peanut rot is peanut rot, the light part of pods are brown or black, the nuts are small and hard, the serious whole pods are dark black, the peels and the nuts are rotted, and the whole plant or the peanut is generally spotted. The yield of the affected peanut land is reduced by 20% if the affected peanut land is light, and the yield can be reduced by more than 50% if the affected peanut land is serious, so that the affected peanut land has serious threat to the yield and quality of peanuts. Fusarium is one of main pathogens causing peanut rot, and is mainly used for preventing and treating the peanut rot by chemical bactericides at present, but the environment pollution is serious, the prevention and treatment effect is poor, and the search for an economic, safe and effective prevention and treatment measure is urgent. The microbial control is an important green control technology in agricultural production, and the antagonistic microorganisms are utilized to control crop diseases, so that the advantages of no pollution, difficulty in causing disease resistance and the like are achieved, and the microbial control has been widely paid attention and applied in recent years.
The key of microbial control is to screen beneficial microorganisms with antagonistic action on pathogenic microorganisms and form a preparation, and the research and application in the biological control aspect of peanut rot are relatively few at present, so that a microbial strain capable of efficiently antagonizing pathogenic bacteria of peanut rot and a related microbial agent are urgently needed to be screened, and the harm of the peanut rot to peanut production is reduced through biological control. At present, no ideal biological agent effective on peanut rot exists.
The bacillus has the characteristics of heat resistance, drought resistance, ultraviolet resistance and the like, and the use of the bacillus for preventing and treating plant diseases is one of the commonly used biological prevention and treatment technologies in agricultural production at present. The bacillus has a plurality of types and quantity, the breeding speed is high, most of the bacillus can be colonized in and out of plants, and the bacillus fermentation process is mature, thereby being convenient for production and application. Bacillus belgii (B.), (Bacillus velezensis)Belongs to a new species of bacillus, is currently used for preventing and treating various plant diseases such as rice blast, cotton verticillium wilt, rice leaf streak, sugarcane scab, lettuce soft rot, pear gray mold, tobacco brown spot, banana wilt, apple rot and the like, but has not found application in preventing and treating peanut rot.
Disclosure of Invention
The invention aims to provide a bacillus belgii strain and a microbial agent taking the bacillus belgii strain as an active ingredient, wherein the microbial agent has an effective prevention and treatment effect on peanut fruit rot.
The Bacillus belgii provided by the invention is Bacillus belgii (B.), (B.), (B.Bacillus velezensis) Hsg1949, the strain has been preserved in China general microbiological culture Collection center (CGMCC) 28.09.2020, with the preservation number of CGMCC No. 20836. The Bacillus beleisi Hsg1949 is obtained by separating and screening more than 1500 soil samples, wherein the soil samples are collected from plots with different degrees of peanut rot in counties such as Hebei famous places, Shijiazhuang places, Baoding places, Qinhuang islands, Tangshan mountains and the like, and the strain has good antagonistic action on fusarium of the peanut rot and can be used for preparing a microbial agent for preventing and treating the peanut rot.
The invention has the beneficial effects that the bacteriostasis rate of the Bacillus beleisi Hsg1949 to the peanut fruit rot strain reaches 58.7-72.9%, and the field control effect on the peanut fruit rot is 1 multiplied by 108~5×108The cfu/mL concentration can reach 56.36-71.24%, which is close to the prevention and treatment level of chemical agents. In addition, it can be used for treating cotton blight, cotton verticillium wilt, cotton wilt and cotton rhizoctonia rotPathogenic bacteria and the like also have certain inhibiting effect. The microbial agent is safe to people and livestock, and has no environmental pollution; the preparation method of the microbial inoculum is simple, convenient to use, low in cost and easy to popularize and apply.
Drawings
FIG. 1 shows the colony morphology of Bacillus belgii Hsg1949 cultured on solid LB medium for 48 hours.
FIG. 2 shows the morphology of Bacillus belgii Hsg1949 after red-dyeing under an optical microscope, wherein a, trophosome; b. and (4) spores.
FIG. 3 is a graph showing the inhibitory effect of Bacillus belgii Hsg1949 on peanut fruit rot.
FIG. 4 is a phylogenetic tree of strain Hsg1949 obtained from the 16S rDNA sequence.
FIG. 5 is a phylogenetic tree of strain Hsg1949 obtained from the gyrA gene sequence.
Detailed Description
The present invention will be further described below with reference to specific embodiments. The experimental methods in the following examples are all conventional methods unless otherwise specified; the percentages in the following examples are by weight unless otherwise specified.
Example 1 isolation and screening of bacteria having antagonistic action against peanut rot
In 10 months in 2019, 1500 parts of soil samples are collected from plots with different degrees of incidence of peanut rot in northern river, famous brand, Shijiazhuang, Baoding, Qinhuang island, Tangshan and other counties and in northern river, and plant protection research of academy of agriculture and forestry in Hebei, and the like, the plots are taken back to a laboratory for bacillus separation, and are subjected to separation and purification for multiple times on an LB (LB) culture medium to obtain pure culture strain laggard, and the strain with high antagonistic performance is screened by adopting a plate confrontation method by taking the peanut rot as an indicator strain.
The method comprises the following specific steps: respectively weighing 1g of the soil sample of the peanut field, placing the soil sample into a sterilized triangular flask filled with 100mL of sterile water, oscillating at 170rpm for 30min, standing for 30min, taking 10mL of supernatant, adding the supernatant into a 50mL sterilized centrifuge tube, carrying out constant-temperature water bath at 80 ℃ for 30min, taking 1mL of supernatant, adding 9mL of sterile water, and obtaining 10mL10-3The suspension of the soil microorganisms is doubled,respectively coating 100 mu L of microbial suspensions with two concentrations on an LB medium plate, repeating each concentration for 3 times, culturing at constant temperature of 30 ℃ for 3-5 days, after the plate grows out of bacteria, screening single bacterial colonies according to morphological characteristics of bacillus in the handbook for identifying common bacteria systems, and performing streak separation and purification until obtaining a pure culture of the single bacterial colonies. Fusarium solani of peanut rot is used as an indicator, and the inhibition rate of different isolates on the growth of the Fusarium is measured by a cross method. The screened bacterial strains are point-connected to 4 corners 3cm away from the center of the plate, activated fusarium peanut rot blocks with the diameter of 5mm are simultaneously inoculated to the center of the plate, each bacterial strain is repeated three times, the bacterial strains are cultured at the constant temperature of 28 ℃ for 5 days, the diameter of the inhibition zone is measured and recorded, and the average inhibition rate is calculated. The strain with the strongest antagonistic action on the fusarium of peanut rot is screened out, and the strain number is Hsg 1949.
Example 2 determination of the genetic stability of antagonistic strains
Subculturing the screened strain Hsg1949 with the strongest antagonistic effect on an LB culture medium by adopting a scribing method, determining the antagonistic effect of the strain on fusarium solani by adopting a plate antagonism method after culturing for 40 generations, taking an original strain as a reference, and culturing the antagonistic strain for 40 generations to have the same antagonistic effect (table 1) as the original strain, thereby indicating that the antagonistic strain has good genetic stability, scraping the antibiotic with stable bacteriostatic effect and placing the antibiotic in a freezing storage tube filled with 1mL of 25% sterile glycerol solution, and storing at-80 ℃.
Example 3 identification of Strain Hsg1949
(1) Morphological characterization
According to the experimental contents and the experimental method recorded in the manual for identifying the common bacteria system, the screened antagonistic strain Hsg1949 is identified, the colony morphology observation is carried out on an LB culture medium, and after the strain is streaked and inoculated, the strain is cultured for 24 hours at the constant temperature of 28 ℃ to observe the colony morphology. Bacterial colonies of the strain Hsg1949 are nearly circular, have a nibbed-etched edge, are protruding and circular in an umbilical protrusion shape, have regular edges, are raised into a steamed bun shape, and have wet surfaces; at the later stage of culture, the surface is dried and has wrinkles, the color is milky white, and the culture medium is not glossy and opaque and is not tightly combined with the culture medium. After being picked and cultured for 48 hours, lawn is observed under an oil microscope with the weight of 100 times of that of the lawn, and under the microscope, the bacterial strain Hsg1949 is bacillus and gram-positive bacteria, the spore is mesogenic or nearly mesogenic, the spore is enlarged, and no parasporal crystal appears singly. These morphological characteristics were substantially identical to those of Bacillus described in the handbook of identification of common bacterial systems (edited by Dongxu bead et al, science publishers, 2001), and it was preliminarily determined that the strain Hsg1949 is Bacillus.
(2) Identification and classification using 16S rDNA sequences
The strain Hsg1949 was inoculated into LB broth, shake-cultured at 28 ℃ for 24 h, with the total DNA extracted as a template, and the DNA sequence of 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID No. 1) and 1492R:
5'-TACGGCTACCTTGTTACGAC TT-3' (SEQ ID No. 2) as primers. The PCR reaction system is 50 ul, and the reaction system is as follows: genomic DNA 2.0. mu.L, 10 XPCR buffer 5.0. mu.L, 27F primer 2. mu.L, 1492R primer 2. mu.L, dNTPs 4. mu.L, Taq enzyme (5U/. mu.L) 0.5. mu.L, ddH2O 34.5.5. mu.L. The reaction conditions are as follows: pre-denaturation at 94 ℃ for 3 min; denaturation at 94 deg.C for 30s, annealing at 54 deg.C for 30s, extension at 72 deg.C for 1.5 min, and circulation for 30 times; 10 min at 72 ℃. The PCR product was detected by electrophoresis on 1% agarose gel, sequenced by Shanghai Biotechnology Ltd, and the obtained DNA sequence (SEQ ID No. 3) was entered into GenBank and analyzed by Blast program in comparison with all sequences in the database. The construction of phylogenetic trees using MEGA software (FIG. 4) showed that strain Hsg1949 aggregated with Bacillus, indicating that strain Hsg1949 belongs to Bacillus.
(3) Identification and classification using gyrA gene sequence
Hsg1949 genome DNA is taken as a template, and a primer gyrA-F of a bacterial housekeeping gene gyrA:
5'-CAGTCAGGAAATGCGTACGTCCTT-3' (SEQ ID No. 4) and
GyrA-R: 5'-CAAGGTAATGCTCCAGGCATTGCT-3' (SEQ ID No. 5) were subjected to PCR amplification. 50 mu l of PCR amplification reaction system of gyrA, wherein the reaction system is as follows: geneGroup DNA 2. mu.L, 10 XPCR buffer 5.0. mu.L, 27F primer 2. mu.L, 1492R primer 2. mu.L, dNTPs 4. mu.L, Taq enzyme (5U/. mu.L) 0.5. mu.L, ddH2O34.5. mu.L. The reaction conditions are as follows: pre-denaturation at 94 ℃ for 3 min; denaturation at 94 deg.C for 30s, annealing at 54 deg.C for 30s, extension at 72 deg.C for 1.5 min, and circulation for 30 times; 10 min at 72 ℃. And (3) delivering the amplified product to Shanghai biological engineering Co., Ltd for sequencing to obtain a gyrA gene sequence (SEQ ID No. 6) of the Hsg1949 strain. The gyrA gene sequences of the obtained strain Hsg1949 were subjected to homology comparison in Genbank. The result shows that the homology of the gene sequence of the gyrA of the strain Hsg1949 and the gene sequence of the gyrA of the Bacillus beilaisi strain NRRL B-41580 is the highest and reaches 99.40 percent; the result (FIG. 5) of using MEGA software to construct phylogenetic trees shows that the strain Hsg1949 is aggregated with Bacillus belgii, indicating that Hsg1949 is Bacillus belgii ((Bacillus belgii))Bacillus velezensis) And is a new strain, unlike known B.belgii strains. The strain Hsg1949 is preserved, and the preservation number is CGMCC No. 20836.
Example 4 antagonistic Effect of Strain Hsg1949 on peanut fruit rot
(1) Sources of peanut rot to be tested: the peanut rot LX, DM and XT strains are respectively from famous counties, Shang counties and 28390of Hebei province, and the disease strains with the peanut rot incidence rate of more than 50% in major producing areas of peanuts in Zhou city are separated and purified by a plant protection research institute of academy of agriculture and forestry of Hebei province and are fusarium by ITS sequence sequencing.
(2) Activating strains: the Bacillus belgii strain Hsg1949 stored at-80 deg.C is streaked on LB plate medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5 g, sodium chloride 1g, agar powder 15g, water 1000 mL), cultured at 30 deg.C for 24 hr, single colony is selected on LB plate medium, cultured at 30 deg.C for 24 hr, and the activated strain is obtained.
(3) The test method comprises the following steps: plate confrontation experiment: firstly, carrying out activated culture on the peanut rot strain on a PDA (personal digital assistant) plate for 5 days, then punching holes on the edge area of a bacterial colony by using a puncher to prepare a bacterial sheet, transferring the peanut rot bacterial cake to the center of another PDA plate, then dotting activated Bacillus beleisi Hsg1949 at a position 2.0cm away from an indicating bacterial cake, and setting a blank control (not dotting the Hsg1949 strain). Culturing at constant temperature of 25 deg.C, when the blank control is about to grow over the whole culture dish, measuring control growth amount (colony radius) and treated growth amount (growth inhibition radius after inoculation of Hsg 1949) of peanut rot, and expressing antagonistic action by antibacterial rate.
The bacteriostatic rate (%) (control growth amount-treated growth amount)/control growth amount × 100.
Results (see table 1, fig. 3) show that the original strain of bacillus belgii Hsg1949 has an inhibition rate of 58.7-72.9% on peanut rot germs from different sources; the transparent bacteriostatic bandwidth is 1.2-8.2 mm; the bacillus beilesensis Hsg1949 has obvious inhibition effect on peanut rot and has biocontrol potential for preventing and treating the peanut rot.
TABLE 1 antagonistic effect test results of Bacillus belgii Hsg1949 on peanut fruit rot
Example 5 preparation of Bacillus beilesiensis Hsg1949 inoculum
(1) Activating strains: the same procedure as in example 4 was used for activating the bacterial strain.
(2) Preparing a seed solution: preparing an LB liquid culture medium (the components and the weight ratio thereof are 1g of tryptone, 0.5 g of yeast extract, 1g of sodium chloride and 1000mL of water) according to a conventional method, filling 50mL of LB culture solution into a 250mL triangular flask, carrying out high-pressure damp-heat sterilization, inoculating the strain activated in the loop inoculation step (1) into each flask after the temperature is reduced to room temperature, and carrying out shaking culture for 24 hours at the temperature of 30 ℃ and the rotation speed of a shaking table of 200rpm to obtain a seed solution.
(3) Preparing a cottonseed meal and soybean meal culture medium: according to the weight percentage, 1.5 percent of cotton seed powder, 2.0 percent of soybean meal, 0.5 percent of NaCl and MnSO4·H2Adding 0.6% of O into water, and stirring and mixing uniformly to obtain a cottonseed meal and soybean meal culture medium; subpackaging in 500mL triangular bottles, 200mL each; sterilizing at 121 deg.C for 30min, and cooling to below 40 deg.C.
(4) Fermentation culture: inoculating 2mL of the seed liquid obtained in the step (2) to the cottonseed meal and soybean meal culture medium obtained in the step (3) according to the proportion of 1% by volume; fermenting and culturing for 36h under the shaking table condition of 30 ℃ and 200rpm, sampling from a triangular flask every 30 minutes, performing microscopic examination, counting spores and total thallus in a visual field, and calculating the spore rate (%) -mature spore number/(mature spore number + thallus number) × 100); stopping fermentation culture when the spore rate reaches more than 90%; and co-fermenting and culturing for about 48 hours to obtain the liquid preparation of the Bacillus belgii Hsg 1949.
Example 6 field application of Bacillus belgii Hsg1949
(1) Experimental treatment
(a) Bacillus belgii Hsg1949 liquid formulation: the final concentration of the fertilizer used in the field is 1 multiplied by 108cfu/mL，2×108cfu/mL，5×108cfu/mL。
(b) Chemical agents: 50 percent carbendazim wettable powder with the application amount of 100 g/mu
(c) Blank control: water (W)
(2) Test method
The test is carried out in peanut fields of Baixin peanut planting professional cooperative, Hebei province, Tangshan city, 28390Zhou city, peanut planting professional cooperative, peanut planting professional, and the peanut rot disease is 15-30% of the peanut rot disease. The peanut variety planted in 5 months in 2020 is Jihua 11, the field is normally managed, the pesticide is sprayed on the root of the peanut in the period of sowing, and Hsg1949 liquid preparation is sprayed on the soil at the root of the peanut, and the using dose is 60 kg/mu; the conventional control uses the chemical pesticide carbendazim with the conventional dosage as the control drug, the using amount of water is 60 kg/mu, and the blank control does not apply the control drug but applies the water with the same weight as the treatment group; each treatment was repeated 3 times to investigate the incidence and yield of peanut rot at the time of peanut harvest.
The investigation method comprises the following steps: sampling at 5 points per treatment, and investigating at 1m point2Total pod weight, total pod number, number of damaged pods were recorded.
The prevention effect calculation method comprises the following steps:
pod morbidity = number of damaged pods/total pods × 100
Control effect (%) = (control area pod morbidity-treatment area pod morbidity)/control area pod morbidity x 100
The control effect is shown in table 2, and field disease prevention tests in Ribei Tangshan city of \28390;. Zhou city show that the Bacillus beleisi Hsg1949 and the microbial agent thereof have good control effect on peanut fruit rot.
TABLE 2 prevention and control of peanut rot by different bacterial agents
Example 7 test of the inhibitory Effect of the Strain Hsg1949 on four soil-borne pathogenic bacteria
(1) Pathogenic bacteria and sources of diseases to be tested: the southern blight bacteria (Sclerotium rolfsii), Verticillium gossypii (Rhizoctonia solani), Fusarium oxysporum (Fusarium oxysporum) and Verticillium dahliae (Verticillium dahliae) tested in this example were isolated and provided by the institute of plant protection of academy of agriculture and forestry, north Heibe.
(2) The test method comprises the following steps: plate confrontation experiment: firstly, each pathogenic bacterium to be tested is activated and cultured on a PDA (personal digital assistant) plate for 5 days, then a puncher is used for punching holes in the edge area of a bacterial colony to prepare a bacterial sheet, a bacterial cake of the pathogenic bacterium to be tested is transferred to the center of the other PDA plate, then the activated Bacillus belgii 1949 in the step (2) in the embodiment 4 is spotted at a position 2.0cm away from an indicating bacterial cake, blank control (not spotted with the Hsg1949 bacterial strain) is set, each treatment is repeated for 3 times, the constant temperature culture is carried out at 25 ℃, observation is carried out day by day, when each pathogenic bacterium blank control is about to grow all over a culture dish, the control growth quantity (bacterial colony radius) and the treatment growth quantity (growth inhibition radius after the Hsg1949 is inoculated) of each pathogenic bacterium are measured, the bacteriostasis rate is calculated, the distance (bacterial zone) between a bacterial inoculation point and the edge of the pathogenic bacterial colony is measured, and the bacteriostasis degree is determined.
Bacteriostatic ratio (%) = (control growth amount-treated growth amount)/control growth amount × 100
TABLE 3 results of experiments on antagonistic action of Bacillus belgii Hsg1949 on plant pathogens
The results (see table 3) show that the bacteriostasis rate of the bacillus beleisi Hsg1949 of the invention to 4 tested pathogenic fungi is 48.0-65.8%, wherein the bacteriostasis rate to cotton verticillium wilt pathogens reaches 65.8%, and the bacteriostasis rate to cotton fusarium wilt pathogens is the lowest, and is 48.0%. The bacterial strain Hsg1949 has obvious inhibition effect on peanut fruit rot, and also has good inhibition effect on cotton fusarium wilt, cotton verticillium wilt and peanut southern blight, and has wide antibacterial spectrum.
Sequence listing
<110> institute of plant protection of academy of agriculture, forestry and science of Hebei province
<120> Bacillus belmentioned Hsg1949 and application thereof
<160> 6
<170> SIPOSequenceListing 1.0
<210> 1
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agagtttgat cctggctcag 20
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tacggctacc ttgttacgac tt 22
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<213> Bacillus belgii (Bacillus velezensis)
<400> 3
ctgggataac tccgggaaac cggggctaat accggatggt tgtttgaacc gcatggttca 60
gacataaaag gtggcttcgg ctaccactta cagatggacc cgcggcgcat tagctagttg 120
gtgaggtaac ggctcaccaa ggcaacgatg cgtagccgac ctgagagggt gatcggccac 180
actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa tcttccgcaa 240
tggacgaaag tctgacggag caacgccgcg tgagtgatga aggttttcgg atcgtaaagc 300
tctgttgtta gggaagaaca agtgccgttc aaatagggcg gcaccttgac ggtacctaac 360
cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg 420
tccggaatta ttgggcgtaa agggctcgca ggcggtttct taagtctgat gtgaaagccc 480
ccggctcaac cggggagggt cattggaaac tggggaactt gagtgcagaa gaggagagtg 540
gaattccacg tgtagcggtg aaatgcgtag agatgtggag gaacaccagt ggcgaaggcg 600
actctctggt ctgtaactga cgctgaggag cgaaagcgtg gggagcgaac aggattagat 660
accctggtag tccacgccgt aaacgatgag tgctaagtgt tagggggttt ccgcccctta 720
gtgctgcagc taacgcatta agcactccgc ctggggagta cggtcgcaag actgaaactc 780
aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc 840
gaagaacctt accaggtctt gacatcctct gacaatccta gagataggac gtccccttcg 900
ggggcagagt gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta 960
agtcccgcaa cgagcgcaac ccttgatctt agttgccagc attcagttgg gcactctaag 1020
gtgactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta 1080
tgacctgggc tacacacgtg ctacaatgga cagaacaaag ggcagcgaaa ccgcgaggtt 1140
aagccaatcc cacaaatctg ttctcagttc ggatcgcagt ctgcaactcg actgcgtgaa 1200
gctggaatcg ctagtaatcg cggatcagca tgccgcggtg aatacgttc 1249
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caaggtaatg ctccaggcat tgct 24
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tcacagacgg attttgtacg caatgaatga tttaggcatg accagtgaca aaccatataa 60
aaaatctgcc cgtatcgtcg gtgaagttat cggtaagtac cacccgcacg gtgactcagc 120
ggtttacgaa tcaatggtca gaatggcgca ggattttaac taccgctaca tgcttgttga 180
cggacacggc aacttcggtt cggttgacgg cgactcagcg gccgcgatgc gttacacaga 240
agcgagaatg tcaaaaatcg caatggaaat cctccgggac attacgaaag atacgattga 300
ttatcaagat aactatgacg gcgcagaaag agaacctgtc gtcatgcctt cgagatttcc 360
gaatctgctc gtaaacggag ctgccggtat tgcggtcgga atggcgacaa atattcctcc 420
gcaccagctt ggggaagtca ttgaaggcgt gcttgccgta agtgagaatc ctgagattac 480
aaaccaggag ctgatggaat acatcccggg cccggatttt ccgactgcag gtcagatttt 540
gggccggagc ggcatccgca aggcatatga atccggacgg ggatccatca caatccgggc 600
taaggctgaa atcgaagaga catcatcagg aaaagaaaga attattgtca cagaacttcc 660
ttatcaggtg aacaaagcga gattaattga aaaaatcgca gatcttgtcc gggacaaaaa 720
aatcgaagga attactgatc tgcgtgacga atccgatcgt aacggaatga gaatcgtcat 780
tgagatccgc cgtgacgcca atgctcacgt cattttgaat aacctgtaca aacaaacggc 840
Claims (4)
1. Bacillus belgii (B.), (Bacillus velezensis) Hsg1949 with preservation number of CGMCC No. 20836.
2. A microbial agent comprising the Bacillus belgii Hsg1949 of claim 1.
3. The microbial agent according to claim 2, wherein the formulation is a liquid formulation, and the concentration of Bacillus belgii Hsg1949 is 1 x 10 when used in the field8~5×108cfu/ mL。
4. The use of a bacillus beilaisi Hsg1949 of claim 1 or a microbial agent of claim 2 or 3 for the control of peanut rot, southern blight, verticillium dahliae or fusarium oxysporum dahliae of peanut.
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| CN112980721B (en) * | 2021-01-29 | 2022-06-10 | 青岛农业大学 | Bacillus belgii and biocontrol preparation and application thereof |
| CN113801826B (en) * | 2021-10-29 | 2023-02-03 | 广西科学院 | Bacillus belgii strain HYL-1 and application thereof |
| CN113943679B (en) * | 2021-11-08 | 2023-02-03 | 南京农业大学 | Bacillus belgii as sheep nasal source and application thereof |
| CN113980871B (en) * | 2021-12-06 | 2023-02-28 | 河南省科学院生物研究所有限责任公司 | Bacillus belgii strain HT-9 and application thereof |
| CN114437972A (en) * | 2022-01-26 | 2022-05-06 | 云南农业大学 | Application of Bacillus beleisi YC89 or metabolite thereof in promoting plant growth |
| CN114703098A (en) * | 2022-04-06 | 2022-07-05 | 陕西嘉益蓝德生物工程有限公司 | Fermentation preparation and application of Bacillus belgii fungicide for preventing and treating cotton wilt |
| CN114874953B (en) * | 2022-06-17 | 2023-04-14 | 河北科技师范学院 | Peanut rhizosphere biocontrol bacterium-Bacillus beleisi SW-1 and application thereof |
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