CN111117640A - Saline-alkali soil improver containing glomus mosseae and improvement method - Google Patents
Saline-alkali soil improver containing glomus mosseae and improvement method Download PDFInfo
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- CN111117640A CN111117640A CN201911345084.1A CN201911345084A CN111117640A CN 111117640 A CN111117640 A CN 111117640A CN 201911345084 A CN201911345084 A CN 201911345084A CN 111117640 A CN111117640 A CN 111117640A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
Abstract
The invention aims at solving the problems of saline-alkali soil improvement in the prior art, and provides a saline-alkali soil improver containing glomus mosseae and an improvement method. The saline-alkali soil modifier consists of Gliocladium moxidense, iron tailings and organic soil, wherein the proportion of the Gliocladium moxidense bacterial liquid to the iron tailings to the organic soil is 2 mL: 2-2.5 g: 1.5-1.8 g, the concentration of the Musaceus liquid is 1-9 multiplied by 107CFU/ml. The invention provides a simpler saline-alkali soil improvement method, which enables saccaromyces mosseae to infect plant root systems to form arbuscular mycorrhizal symbiont, and utilizes iron tailings as a modifier to control saltThe alkaline earth planting plants play a synergistic role, and the biomass of the plants is improved while the purposes of improving the saline-alkali soil and improving the availability of the saline-alkali soil are achieved.
Description
Technical Field
The invention belongs to the field of saline-alkali soil improvement, and particularly relates to a coastal saline-alkali soil improver utilizing iron tailings and microorganisms and an improvement method.
Background
Saline-alkali soil is a soil harmful to the normal growth of crops and is a generic term for saline soil, alkaline earth and other salinized and alkalized soils. Generally, the salt content of saline-alkali soil is more than 0.1-0.2%, or soil colloid adsorbs a certain amount of exchangeable sodium, the alkalization degree is more than 15-20%, and soil is easy to harden under the drought condition to reduce water permeability and air permeability, so that the absorption of water and nutrients by plants is seriously influenced, and the growth and development of the plants are extremely unfavorable. At present, the development of saline-alkali soil treatment and improvement technology is more and more paid attention by people.
The technology for improving the saline-alkali soil mainly comprises physical, chemical and biological technologies, and compared with the physical and chemical technologies, the biological improvement technology has the characteristics of lower cost, simplicity and convenience in operation, difficulty in secondary pollution and the like, the current research on the saline-alkali soil biological improvement technology mainly adopts saline-alkali resistant microbial fertilizers, microbial inoculum adding and the like for improvement, for example, the Chinese patent of application No. CN201610860371.6 discloses a biological drip irrigation saline-alkali soil improver and a preparation method, the biological drip irrigation saline-alkali soil improver is prepared from amino acid, potassium fulvate, urea, monoammonium phosphate, potassium nitrate and dry powder microbial inoculum, the Chinese patent of application No. CN201811178532.9 discloses a method for improving the saline-alkali soil by cordyceps fermentation broth, and the steps are as follows, ⑴ the cordyceps fermentation broth is subjected to high-temperature inactivation treatment, ⑵ straw or sawdust and the organic fertilizer are uniformly mixed to be used as a solid adsorption carrier, ⑶ the solid adsorption carrier and the cordyceps fermentation broth are prepared into a mixture, ⑷ bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae and trichoderma viride are prepared into the mixture to obtain a relatively weak alkaline earth improver which is uniformly mixed to improve the soil improvement effect.
The sacculus moscillus is one of arbuscular mycorrhizal fungi, and can be symbiotic with part of plants to improve the yield of the plants. Chinese patent application No. CN201010508569.0 utilizes glomus mosseae to reduce vegetable phoxim residues. However, a method for improving saline-alkali soil by using saccaromyces morseli and iron tailings together is not reported.
Disclosure of Invention
The invention aims at solving the problems of saline-alkali soil improvement in the prior art, and provides a saline-alkali soil improver containing glomus mosseae and an improvement method. The invention provides a simpler saline-alkali soil improvement method, which enables saccaromyces morselii to infect plant root systems to form arbuscular mycorrhizal symbionts, utilizes iron tailings as a modifier to play a synergistic role in saline-alkali soil planting plants, achieves the purposes of improving the saline-alkali soil and improving the utilization rate of the saline-alkali soil, and simultaneously improves the plant biomass.
The saline-alkali soil modifier containing the Mucillus mosseae consists of the Mucillus mosseae, iron tailings and an organic fertilizer, wherein the proportion of Mucillus mosseae liquid, the iron tailings and the organic fertilizer is 2 mL: 2-2.5 g: 1.5-1.8 g, the concentration of the Musaceus liquid is 1-9 multiplied by 107CFU/ml；
The saline-alkali soil modifier has the preservation number of glomus mosseae as follows: CGMCC NO.11662, from China general microbiological culture Collection center.
The granularity of the iron tailings of the saline-alkali soil modifier is less than or equal to 20 mu m;
the saline-alkali soil modifier is characterized in that the organic fertilizer is a commercially available organic fertilizer.
The saline-alkali soil improvement method adopts the improver and comprises the following specific steps:
1) application of iron tailings
Adding 2000-2500 g of iron tailings and 1500-1800 g of organic fertilizer into per square meter of soil, adding the iron tailings and the organic fertilizer into the coastal saline-alkali soil, ploughing to a depth of 20-30 cm, and then performing flood irrigation on the saline-alkali soil to balance the soil for 5-10 days;
2) plant cultivation
Sowing plant seeds into soil, and watering regularly;
3) application of Musaceus mossbiensis liquid
To be plantedAfter the seeds germinate, the concentration is 1-9 multiplied by 107Spraying the CFU/ml Moses sacculus mildew liquid according to the dosage of 2000ml per square meter, so that the Moses sacculus mildew infects plant root systems to form arbuscular mycorrhizal symbionts; and (4) performing conventional management on the plants, and enabling the plants to grow normally until the plants are harvested.
In the saline-alkali soil improvement method, the plants in the step 2) are leguminous plants capable of forming arbuscular mycorrhiza with sacculus mosissima, such as medicago truncatula, medicago sativa and crowtoe;
in the saline-alkali soil improvement method, in the step 2), plant seeds with full grains and complete plumules are selected, and the seeds are soaked in water for 3-4 hours before sowing.
Compared with the prior art, the invention has the advantages that:
(1) according to the invention, the proportion of the Mucillus mossi microbial inoculum and the iron tailings is selected, so that the saline-alkali soil can be well improved, the biomass of plants is increased, the construction is convenient, the action effect lasts for a long time, and the environment quality of the repaired soil meets the requirements of the soil environment quality standard GB 156181995 in China.
(2) The invention uses the iron tailing waste material as the modifier, has wide material source, low cost and simple manufacturing process, and realizes the recycling of waste resources. Aiming at the characteristics of high yield, low utilization rate and the like of the iron tailings, the method can apply a large amount of iron tailings to the restoration of saline-alkali soil, so that the iron tailings are changed into valuable.
(3) The overlapping effect of double electric layers between the iron tailing colloid and the soil colloid is beneficial to soil desalination. The iron tailing colloid surface net positive charge, when soil colloid and iron tailing suspension mix dispersion and form colloidal suspension in aqueous, because electrostatic action, the soil colloid of negative charge and the iron tailing colloid of positive charge can be close to each other, the diffusion barrier of soil colloid and iron tailing surface electric double layer can overlap each other, the overlap of diffusion barrier can make colloidal particle surface partial charge offset each other, the effective negative charge density on soil colloid surface and the effective positive charge density on iron tailing surface reduce, thereby reduce the absorption of soil to salt ions such as sodion, realize the desalination.
(4) The method has the advantages of simple process, low cost, environmental protection and wide application range.
Detailed Description
The preparation method of the saccaromyces mosseae bacterial liquid used in the following examples is as follows:
(1) propagation of Musicella morsela
Activating saccule mosaici on a common broth slope to obtain activated strains; inoculating the activated test strain into a conical flask filled with beef extract peptone culture medium and subjected to high-temperature sterilization treatment for propagation, and performing propagation at 28-30 ℃ for 200-220 r.min-1Oscillating the mixture on a shaking table for 18 to 24 hours, and then diluting the bacteria solution to 1 to 9 multiplied by 10 by using sterile water7CFU/ml (count on haemocytometer).
Wherein, the formula of the common broth comprises: 20.0g of peptone, 5.0g of NaCl and 5.0g of beef powder, adjusting the pH value to 7.5, and metering the volume to 1000 ml. The formula of the beef extract peptone culture medium is as follows: 10.0g of peptone, 5.0g of NaCl and 5.0g of beef extract, adjusting the pH to be the same as the pH of soil, fixing the volume with 1000ml of sterile water, and proportionally increasing the volume according to the actual dosage.
The iron tailings are iron tailings with the granularity of 20 mu m in the Anshan Dalonshan iron tailings pond; the organic fertilizer is chicken manure sold in the market.
Example 1
The saline-alkali soil modifier containing the Mucillus mosseae consists of the Mucillus mosseae, iron tailings and an organic fertilizer, wherein the proportion of Mucillus mosseae liquid, the iron tailings and the organic fertilizer is 2 mL: 2.5 g: 1.8g, the concentration of the Mucor saceus bacterial liquid is 4.5 multiplied by 107CFU/ml。
The saline-alkali soil improvement method comprises the following steps:
1) adding 2500g of iron tailings and 1800g of organic fertilizer into each square meter of soil, adding the iron tailings and the organic fertilizer into saline-alkali soil (Haixing county of Cangzhou city in Hebei province), turning over for 25cm, and then, flood-irrigating the saline-alkali soil, and balancing the soil for one week.
2) Plant cultivation
Selecting full-grain alfalfa seeds with complete germs, soaking the seeds in water for 4 hours, then sowing the seeds in soil, and watering the seeds regularly.
3) Application of Gliocladium mosseae inoculum
After the alfalfa seeds germinate, the concentration is 4.5 multiplied by 107The Moses sacculus mildew agent of CFU/ml is sprayed according to the dosage of 2000ml per square meter, so that the Moses sacculus mildew infects plant root systems to form arbuscular mycorrhizal symbiont.
4) And performing conventional management on the alfalfa, and harvesting the alfalfa after the alfalfa normally grows for 2 months.
Comparative example 1
The method for restoring the coastal saline-alkali soil by using the iron tailings comprises the following steps:
the procedure is as in example 1, except that no glomus mosseae is applied.
Comparative example 2
The method for repairing the coastal saline-alkali soil by using the Mucillus mosseae comprises the following steps:
the process is the same as example 1 except that no iron tailings are added.
Comparative example 3
The method for restoring the coastal saline-alkali soil by utilizing the saline-alkali soil modifier comprises the following steps:
the method is the same as example 1 except that the plant to be planted is amaranth (no arbuscular mycorrhiza formation).
After the plants are harvested, the physical and chemical properties of the soil and the physical and chemical properties of the plants are measured, saline-alkali soil without any modifier is used as a blank control group, and the experimental measurement results are shown in tables 1 and 2:
TABLE 1 plant indices
Table 2: soil index
In the plant indexes, compared with a control group, the plant height, the plant dry weight, the SPAD value of leaves and the crude protein of the comparative example 1 are increased by 1.62, 4.07, 1.18 and 1.32 times respectively; in the soil index, the pH value, the conductivity and the salt content of the soil are respectively reduced by 6.73%, 16.60% and 22.59% in comparison with those of the control group in comparative example 1.
In the plant indexes, compared with a control group, the plant height, the plant dry weight, the SPAD value of leaves and the crude protein of the comparative example 2 are increased by 1.58, 3.97, 1.11 and 1.24 times respectively; in the soil index, the pH value, the conductivity and the salt content of the comparative example 2 are respectively reduced by 5.78%, 14.10% and 18.41% compared with those of the control group.
In the plant indexes, compared with a control group, the plant height, the plant dry weight, the SPAD value of leaves and the crude protein of the plant of the example 1 are increased by 2.03 times, 4.74 times, 1.51 times and 1.64 times respectively; in the soil index, the pH value, the conductivity and the salt content of the soil are respectively reduced by 15.67%, 40.45% and 49.37% in example 1 compared with those of a control group.
In the soil indexes, compared with example 1, the pH value, the conductivity and the salt content of comparative example 3 are respectively increased by 11.35%, 59.51% and 59.50%.
In summary, in the plant indexes and the soil indexes, the sacculus moseri and the alfalfa form arbuscular mycorrhizal symbionts in the example 1, the saline-alkali soil is mixed with the iron tailings to form a synergistic effect on soil improvement, compared with the comparative examples 1-3, the growth condition is the best, the stress resistance is the strongest, the pH of the soil can be reduced by 15.67% in two months by the method in the example 1, and the treatment period can be effectively shortened.
Example 2
The saline-alkali soil modifier containing the Mucillus mosseae consists of the Mucillus mosseae, iron tailings and an organic fertilizer, wherein the proportion of Mucillus mosseae liquid, the iron tailings and the organic fertilizer is 2 mL: 2 g: 1.5g, the concentration of the Mucor saceus bacterial liquid is 8.7 multiplied by 107CFU/ml。
The saline-alkali soil improvement method comprises the following steps:
1) adding 2000g of iron tailings and 1500g of organic fertilizer into each square meter of soil, adding the iron tailings and the organic fertilizer into saline-alkali soil (Haixing county of Cangzhou city in Hebei province), and after ploughing, flood-irrigating the saline-alkali soil, and balancing the soil for 5 days.
2) Plant cultivation
Selecting full-grain alfalfa seeds with complete germs, soaking the seeds in water for 3 hours, then sowing the seeds in soil, and watering the seeds regularly.
3) Application of Gliocladium mosseae inoculum
After the alfalfa seeds germinate, the concentration is 8.7 multiplied by 107The CFU/ml microbial inoculum is sprayed according to the dosage of 2000ml per square meter, so that the saccaromyces mosseae infects the root system of the plant to form arbuscular mycorrhizal symbiont.
4) And performing conventional management on the medicago sativa to ensure that the medicago truncatula grows normally to be mature and then is harvested.
Example 3
The saline-alkali soil modifier containing the Mucillus mosseae consists of the Mucillus mosseae, iron tailings and an organic fertilizer, wherein the proportion of Mucillus mosseae liquid, the iron tailings and the organic fertilizer is 2 mL: 2.2 g: 1.6g, the concentration of the Mucor saceus bacterial liquid is 1.2 multiplied by 107CFU/ml。
The saline-alkali soil improvement method comprises the following steps:
1) adding 2200g of iron tailings and 1600g of organic fertilizer into saline-alkali soil (Haixing county of Cangzhou city in Hebei province) according to the proportion of adding the iron tailings and the organic fertilizer into per square meter of soil, and after ploughing, flood-irrigating the saline-alkali soil and balancing the soil for 10 days.
2) Plant cultivation
Selecting crowtoe seeds with full particles and complete germs, soaking the seeds in water for 4 hours, then sowing the seeds in soil, and watering the seeds regularly.
3) Application of Gliocladium mosseae inoculum
After the Lotus corniculatus seeds germinate, the concentration is 1.2 multiplied by 107And spraying the CFU/ml microbial inoculum according to the specification of 2000ml per square meter to ensure that the sacculus moxidensis infects plant root systems to form arbuscular mycorrhizal symbionts.
4) And (4) performing conventional management on the crowtoe, and harvesting the crowtoe after the crowtoe normally grows to be mature.
Claims (9)
1. The saline-alkali soil modifier containing the Muscovitum sacculus mildew is characterized by consisting of the Muscovitum sacculus mildew, iron tailings and organic soil, wherein the proportion of Muscovitum sacculus mildew liquid, the iron tailings and the organic soil is 2 mL: 2-2.5 g: 1.5-1.8 g.
2. The saline-alkali soil improver according to claim 1, wherein the concentration of the saccaromyces mosseae solution is 1 to 9 x 107CFU/ml。
3. The saline-alkaline earth improver according to claim 1, wherein the glomus mosseae has a deposit number of: CGMCC NO.11662, from China general microbiological culture Collection center.
4. The saline-alkaline-earth improver according to claim 1, wherein the particle size of the iron tailings is 20 μm or less.
5. The saline-alkali soil improver according to claim 1, wherein the organic soil is a commercially available organic fertilizer.
6. The saline-alkali soil improvement method adopts the saline-alkali soil improvement agent disclosed by claim 1, and is characterized by comprising the following specific steps of:
1) application of iron tailings
Adding 2000-2500 g of iron tailings and 1500-1800 g of organic fertilizer into per square meter of soil, adding the iron tailings and the organic fertilizer into the coastal saline-alkali soil, ploughing to a depth of 20-30 cm, and then performing flood irrigation on the saline-alkali soil to balance the soil for 5-10 days;
2) plant cultivation
Sowing plant seeds into soil, and watering regularly;
3) application of Musaceus mossbiensis liquid
After the plant seeds germinate, the concentration is 1-9 multiplied by 107Spraying the CFU/ml Moses sacculus mildew liquid according to the dosage of 2000ml per square meter, so that the Moses sacculus mildew infects plant root systems to form arbuscular mycorrhizal symbionts; and (4) performing conventional management on the plants, and enabling the plants to grow normally until the plants are harvested.
7. The method for improving saline-alkali soil according to claim 6, wherein the plant in the step 2) is a plant capable of forming arbuscular mycorrhiza with Gliocladium moxidense in Leguminosae.
8. The method for improving saline-alkali soil according to claim 6, wherein the plants in step 2) are Medicago truncatula, Medicago sativa, and Lotus corniculatus.
9. The method for improving saline-alkali soil according to claim 6, wherein in the step 2), plant seeds with full grains and complete germs are selected, and the seeds are soaked in water for 3-4 hours before sowing.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113388528A (en) * | 2021-06-15 | 2021-09-14 | 中国林业科学研究院林业研究所 | Ground surface sacculus mould, composition for saline-alkali soil remediation and saline-alkali soil remediation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113388528A (en) * | 2021-06-15 | 2021-09-14 | 中国林业科学研究院林业研究所 | Ground surface sacculus mould, composition for saline-alkali soil remediation and saline-alkali soil remediation method |
| CN113388528B (en) * | 2021-06-15 | 2022-07-08 | 中国林业科学研究院林业研究所 | Ground surface sacculus mould, composition for saline-alkali soil remediation and saline-alkali soil remediation method |
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Application publication date: 20200508 |