CN110122208A - A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi - Google Patents
A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi Download PDFInfo
- Publication number
- CN110122208A CN110122208A CN201910502087.5A CN201910502087A CN110122208A CN 110122208 A CN110122208 A CN 110122208A CN 201910502087 A CN201910502087 A CN 201910502087A CN 110122208 A CN110122208 A CN 110122208A
- Authority
- CN
- China
- Prior art keywords
- dandelion
- soil
- flowerpot
- mycorrhizal fungi
- arbuscular mycorrhizal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi, is related to improving dandelion to the tolerance technical field of salt stress.The invention aims to solve the problems, such as how to improve dandelion to the tolerance of salt stress.Method: will be added soil, then the mixed soil of the soil stirred evenly and arbuscular mycorrhizal fungi be added into each flowerpot, Taraxacum mongolicum seeds are sowed into flowerpot in flowerpot, then the upper soil that tiles in flowerpot；The flowerpot for sowing Taraxacum mongolicum seeds is placed in culturing room and is cultivated, culture environment: light application time is 12h/d~16h/d, and intensity of illumination is 4800lx~5200lx, and temperature is 23 DEG C~25 DEG C, relative air humidity is 60%~70%, and keeping the water content of soil in each flowerpot is 55%~70%.The present invention can get a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability.
Description
Technical field
The present invention relates to improve dandelion to the tolerance technical field of salt stress.
Background technique
For agricultural land soil safety problem concerning the people's livelihood, soil salinization problem is quite serious in China, causes a large amount of arable lands
It degenerates, constrains China's agricultural production.Effective exploitation using saline and alkaline soil be one concerning the people's livelihood the problem of.Salt-soda soil belongs to
The toxic action of one of special habitats, alkalinity with high salt is suppressed plant growth and development, or even dead, to influence agriculture
The sustainable development of forestry and animal husbandry production and Productivity of Ecological System.The soil salinization about influences 8% soil in the world
Ground area is a great global soil degradation environmental problem.Since the soil salinization area in the whole world is just with great
Speed increases, and increased tilth salinization problem will cause devastating impact to the whole world, it is contemplated that will lead in future 25 years
30% soil loss may reduce half because of the soil salinization to the available soil in the 21 century mid-term whole world.China's salt
Stain soil area (9.9133 × 107Hm it) ranked third in the whole world, account about the 10% of global salinized soil area.In recent years, by life
Object recovery technique is subject to the thought that certain assisted and strengthened means carries out saline and alkaline land remediation and accepts extensively for people, with biological skill
Art improvement salt-affected soil and soil sustainable utilization also receive the extensive concern of scientific research circle.
Arbuscular mycorrhizal fungi (Arbuscular mycorrhizal fungi, AMF) is generally distributed in exist including salt-soda soil
It in interior all kinds of ecological environment of soil, and is one of maximum composition of biomass in soil microbial community.AM fungi conduct
Generally existing one kind can form the endogenetic fungus of non-specific symbiosis with most plants in nature, can help
Host plant improves impoverishment tolerant ability, nutrient needed for helping host to obtain and minerals, in the energy for improving plant anti-salt alkaline stress
Power simultaneously promotes have irreplaceable role in plant growth and development.
Dandelion (Taraxacum mongolicum) also known as grandmother's fourth, Rhizoma Trillii Tschonoskii etc., are herbaceos perennials, are belonged to
It in composite family Dandelion, is often used as drug, there is clearing heat and detoxicating effect；Meanwhile containing a large amount of nutrition in dandelion body
Ingredient and its taste is still good, becomes civil one of the fashionable edible edible wild herbs in China, is liked.Dandelion is resisted cold drought-enduring, growing way
Powerful, form is graceful, and annidation is strong, is almost distributed throughout our country.Dandelion is in salt tolerant level, quilt
It is considered high salt-tolerant plant.When salinity is 0.2%, the growth of dandelion is not only not inhibited, and facilitates seedling instead
It survives, when salinity is higher than 0.2%, the growth of dandelion is suppressed.
Currently, solonchak area is extensive, soil salinization problem is more serious, in order to guarantee that agricultural production and the reclamation of wasteland do not utilize
Saline and alkaline soil, the problem of urgent need to resolve is how to improve plant to the tolerance of salt stress.Also, by Mycorrhizal Technology
It yet there are no report applied to research of the dandelion in terms of the growth under salt stress is improved.
Summary of the invention
The invention aims to solve the problems, such as how to improve dandelion to the tolerance of salt stress, and provide one kind
Utilize the method for arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability.
A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi, complete according to the following steps:
One, inoculation is handled: 130 parts~140 parts soil will first be added in several flowerpots, then by 260 parts~280 parts soil
Earth and 28 parts~32 parts arbuscular mycorrhizal fungi are stirring evenly and then adding into each flowerpot；
Two, Taraxacum mongolicum seeds are sowed: Taraxacum mongolicum seeds being sowed into step 1 in each flowerpot, and adjacent dandelion
Seed spacing is identical, then upper 20 parts~30 parts soil that tile in each flowerpot；
Three, Taraxacum mongolicum seeds culture: the flowerpot of sowing Taraxacum mongolicum seeds all in step 2 being placed in culturing room and is cultivated,
Culture environment: light application time is 12h/d~16h/d, and intensity of illumination is 4800lx~5200lx, and temperature is 23 DEG C~25 DEG C, empty
Gas relative humidity is 60%~70%, and keeping the water content of soil in each flowerpot is 55%~70%.
Beneficial effects of the present invention:
A kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability of the present invention, in the soil of cultivation dandelion
Middle inoculation Glomus mosseae has turned out the dandelion of inoculation Glomus mosseae, by being provided with 5 NaCl concentrations in experiment
Gradient (respectively 0,100mmol/L, 200mmol/L, 300mmol/L, 400mmol/L) respectively to inoculation Glomus mosseae
Dandelion and be not inoculated with Glomus mosseae dandelion carry out salt stress test, the results showed that under salt stress, be inoculated with Moses
The mould biomass for improving dandelion of sacculus and plant height improve the absorption of dandelion internal water and nutrition condition, increase leaf
Chlorophyll contents and foliar SOD, POD and CAT activity are improved, while reducing MDA content in dandelion body, the absorption of Na, dried meat
The size of histidine content and relative conductivity illustrates that Glomus mosseae acts on significantly in terms of promoting dandelion salt resistance ability.
A kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability of the present invention, simulates salt stress using potting
Experiment, probes into Glomus mosseae (G.mosseae) to the growth of dandelion, moisture absorption, element absorption, physiology under salt stress
Metabolism and the influence of antioxidase, it is intended to the related mechanism that arbuscular mycorrhizal fungi improves salt tolerance of dandelion is disclosed, to be bacterium
Root-dandelion syntaxial system repairs salt-affected soil and provides theoretical foundation.
The present invention can get a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability.
Detailed description of the invention
Fig. 1 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the ground of the dandelion of Glomus mosseae processing
The datagram of top biomass；
Fig. 2 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the root of the dandelion of Glomus mosseae processing
It is the datagram of biomass；
Fig. 3 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the strain of the dandelion of Glomus mosseae processing
High datagram；
Fig. 4 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the P member of the dandelion of Glomus mosseae processing
The datagram of cellulose content；
Fig. 5 is be not inoculated with dandelion that Glomus mosseae and inoculation Glomus mosseae are handled auxiliary under five stress gradients
The datagram of histidine content；
Fig. 6 is not to be inoculated with the phase of Glomus mosseae with the dandelion for being inoculated with Glomus mosseae processing under five stress gradients
To the datagram of conductivity；
Fig. 7 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the super of the dandelion of Glomus mosseae processing
The datagram of superoxide dismutase content；
Fig. 8 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the mistake of the dandelion of Glomus mosseae processing
The datagram of oxide enzyme content；
Fig. 9 is be not inoculated with dandelion that Glomus mosseae and inoculation Glomus mosseae are handled red under five stress gradients
The datagram of sanguinin enzyme content；
Figure 10 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the leaf of the dandelion of Glomus mosseae processing
The datagram of chlorophyll contents；
Figure 11 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the group of the dandelion of Glomus mosseae processing
Knit the datagram of water content；
Figure 12 is not to be inoculated with the third of the dandelion that Glomus mosseae and inoculation Glomus mosseae are handled under five stress gradients
The datagram of dialdehyde content；
Figure 13 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the Na of the dandelion of Glomus mosseae processing
The datagram of constituent content.
Wherein, in Fig. 1~Figure 13,1 indicates the dandelion for not being inoculated with Glomus mosseae processing, and 2 indicate inoculation Moses's sacculus
The dandelion of mould processing, * indicate the significance of difference.
Specific embodiment
Specific embodiment 1: a kind of side using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability of present embodiment
Method is completed according to the following steps:
One, inoculation is handled: 380 parts~420 parts soil will first be added in several flowerpots, then in the soil of each flowerpot
Tile upper 28 parts~32 parts arbuscular mycorrhizal fungi on earth upper layer, then tiles upper 25 parts~30 in the arbuscular mycorrhizal fungi of each flowerpot
Part soil；
Two, Taraxacum mongolicum seeds are sowed: Taraxacum mongolicum seeds being sowed into step 1 in each flowerpot, and adjacent dandelion
Seed spacing is identical, then upper 80 parts~100 parts soil that tile in each flowerpot；
Three, Taraxacum mongolicum seeds culture: the flowerpot of sowing Taraxacum mongolicum seeds all in step 2 being placed in culturing room and is cultivated,
Culture environment: light application time is 12h/d~16h/d, and intensity of illumination is 4800lx~5200lx, and temperature is 23 DEG C~25 DEG C, empty
Gas relative humidity is 60%~70%, and keeping the water content of soil in each flowerpot is 55%~70%.
Present embodiment the utility model has the advantages that
A kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability of present embodiment, in cultivation dandelion
It is inoculated with Glomus mosseae in soil, has turned out the dandelion of inoculation Glomus mosseae, by being provided with 5 NaCl in experiment
The gradient (respectively 0,100mmol/L, 200mmol/L, 300mmol/L, 400mmol/L) of concentration is respectively to inoculation Moses's ball
The mould dandelion of capsule and the dandelion for not being inoculated with Glomus mosseae carry out salt stress test, the results showed that under salt stress, inoculation
Glomus mosseae improves the biomass and plant height of dandelion, improves the absorption of dandelion internal water and nutrition condition, increases
Chlorophyll content and foliar SOD, POD and CAT activity are improved, while reducing the suction of MDA content, Na in dandelion body
It receives, the size of proline content and relative conductivity, it is aobvious to illustrate that Glomus mosseae acts in terms of promoting dandelion salt resistance ability
It writes.
A kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability of present embodiment, utilizes potting analog salt
Stress experiment, probe into Glomus mosseae (G.mosseae) to the growth of dandelion under salt stress, moisture absorption, element absorption,
Physiological metabolism and the influence of antioxidase, it is intended to the related mechanism that arbuscular mycorrhizal fungi improves salt tolerance of dandelion is disclosed, to
Salt-affected soil is repaired for mycorhiza-dandelion syntaxial system, and theoretical foundation is provided.
Specific embodiment 2: the differences between this implementation mode and the specific implementation mode are that: the soil is nutrition
Soil, Nutrition Soil are made of river sand, vermiculite and garden mould, and the mass ratio of river sand, vermiculite and garden mould is 1:1:3.
Other steps are same as the specific embodiment one.
Specific embodiment 3: present embodiment is with specific embodiment one or two differences: described in step 1
Soil is before use, the 2h~2.5h that sterilizes under the conditions of 121 DEG C~122 DEG C of temperature.
Other steps are the same as one or two specific embodiments.
Specific embodiment 4: one of present embodiment and specific embodiment one to three difference are: institute in step 1
The bottom for the flowerpot stated has permeable hole.
Other steps are identical as specific embodiment one to three.
Specific embodiment 5: one of present embodiment and specific embodiment one to four difference are: will in step 1
260 parts~280 parts soil and 30 parts of arbuscular mycorrhizal fungi are stirring evenly and then adding into each flowerpot.
Other steps are identical as specific embodiment one to four.
Specific embodiment 6: one of present embodiment and specific embodiment one to five difference are: institute in step 1
The arbuscular mycorrhizal fungi stated is Glomus mosseae.
Other steps are identical as specific embodiment one to five.
Specific embodiment 7: one of present embodiment and specific embodiment one to six difference are: Pu in step 2
Before public English seed sowing, it is placed in the KMnO that mass fraction is 0.2%~0.4%428min~32min is sterilized in solution.
Other steps are identical as specific embodiment one to six.
Specific embodiment 8: one of present embodiment and specific embodiment one to seven difference are: every in step 2
A flowerpot sows 10 Taraxacum mongolicum seeds.
Other steps are identical as specific embodiment one to seven.
Specific embodiment 9: one of present embodiment and specific embodiment one to eight difference are: in step 2
Tile upper 25 parts of soil in each flowerpot.
Other steps are identical as specific embodiment one to eight.
Specific embodiment 10: one of present embodiment and specific embodiment one to nine difference are: being trained in step 3
Environment: light application time 14h/d, intensity of illumination 5000lx is supported, temperature is 24 DEG C, relative air humidity 65%, and is kept
The water content of soil is 60% in each flowerpot.
Other steps are identical as specific embodiment one to nine.
Beneficial effects of the present invention are verified using following embodiment:
A kind of embodiment one: method (i.e. inoculation Moses's sacculus using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability
The cultural method of mould dandelion), it completes according to the following steps:
One, inoculation is handled: 40 bottoms being had in the flowerpot of permeable hole, 130g soil is first added, then by 270g
Soil and 30g arbuscular mycorrhizal fungi are stirring evenly and then adding into each flowerpot；
Glomus mosseae (G.mosseae) described in step 1, average spore density are 18/g soil, and purchase is certainly northern
Jing Nonglinkexueyuan, deposit number: 1511C0001BGCAM0010；
Soil described in step 1 is Nutrition Soil, and Nutrition Soil is made of river sand, vermiculite and garden mould, river sand, vermiculite and
The mass ratio of garden mould is 1:1:3；
Soil described in step 1 is before use, the 2h that sterilizes under the conditions of 121 DEG C of temperature；
Two, Taraxacum mongolicum seeds are sowed: Taraxacum mongolicum seeds being sowed into step 1 in each flowerpot, and adjacent dandelion
Seed spacing is identical, and each flowerpot sows 10 Taraxacum mongolicum seeds, then the upper 25g soil that tiles in each flowerpot；
Before the sowing of Taraxacum mongolicum seeds described in step 2, it is placed in the KMnO that mass fraction is 0.3%4It is sterilized in solution
30min；
Three, Taraxacum mongolicum seeds culture: the flowerpot of sowing Taraxacum mongolicum seeds all in step 2 being placed in culturing room and is cultivated,
Culture environment: light application time 14h/d, intensity of illumination 5000lx, temperature are 24 DEG C, relative air humidity 65%, and
The water content for keeping soil in each flowerpot is 60%；
Four, Taraxacum mongolicum seeds break ground latter week of sprouting, and three plants of dandelion seedling are left in each flowerpot, and extra Pu is public
English seedling is pulled out.
The Glomus mosseae described in step 1 of embodiment one, belongs to Paraglomus, and spore feature is light yellow, color
Deep is in yellowish-brown, spherical or subsphaeroidal, 3 layers of conidial cell wall.The bacterial strain is dandelion root system dominant strain, has centainly resistance to
The soil of salt ability, slight alkalinity is conducive to the bacterial strain and dandelion symbiosis and the growth of bacterial strain；
Comparative example one: the cultural method for not being inoculated with the dandelion of Glomus mosseae is completed by the following steps:
One, it is not inoculated with processing: 40 bottoms being had, 430g soil is first added in the flowerpot of permeable hole；
Soil described in step 1 is Nutrition Soil, and Nutrition Soil is made of river sand, vermiculite and garden mould, river sand, vermiculite and
The mass ratio of garden mould is 1:1:3；
Soil described in step 1 is before use, the 2h that sterilizes under the conditions of 121 DEG C of temperature；
Two, Taraxacum mongolicum seeds are sowed: Taraxacum mongolicum seeds being sowed into step 1 in each flowerpot, and adjacent dandelion
Seed spacing is identical, and each flowerpot sows 10 Taraxacum mongolicum seeds, then the upper 25g soil that tiles in each flowerpot；
Before the sowing of Taraxacum mongolicum seeds described in step 2, it is placed in the KMnO that mass fraction is 0.3%4It is sterilized in solution
30min；
Three, Taraxacum mongolicum seeds culture: the flowerpot of sowing Taraxacum mongolicum seeds all in step 2 being placed in culturing room and is cultivated,
Culture environment: light application time 14h/d, intensity of illumination 5000lx, temperature are 24 DEG C, relative air humidity 65%, and
The water content for keeping soil in each flowerpot is 60%；
Four, Taraxacum mongolicum seeds break ground latter week of sprouting, and three plants of dandelion seedling are left in each flowerpot, and extra Pu is public
English seedling is pulled out.
Embodiment two:
Salt stress processing: after the dandelion cultivated in example one and comparative example one to be performed grows into 60 days, will own
Dandelion carries out salt stress processing, and it is 100mmol/L, 200mmol/L, 300mmol/L, 400mmol/L that concentration is arranged altogether
Five stress gradients of NaCl solution and distilled water, all dandelions averagely carry out salt stress processing according to five stress gradients, with
Distilled water pours the NaCl solution or distilled water of 20mL various concentration at interval of 1 day every basin as control treatment, dandelion.
Dandelion plant in the step of embodiment one and comparative example one four is taken out from flowerpot, dandelion is planted
Root system and its overground part of strain separate, and after root system is cleaned, root system are placed in FAA fixer and is saved under conditions of 4 DEG C；
When microscopy, dandelion root system is taken out from FAA fixer and is cut into the root segment that size is about 1cm long, is then placed in and fills matter
In the small beaker for measuring the KOH that score is 10%, beaker is put into disappearing for 60min of progress in 90 DEG C of thermostat water baths and is boiled, after cooling
Pour out KOH solution, by distilled water flushing root system 3 times, while with mass fraction be 2% hydrochloric acid be acidified 5min after, by dandelion
Root system and 0.05% trypan blue solution be placed in 90 DEG C of water-baths dye 30min after, coloring agent is poured out, be added lactic acid it is sweet
Oil solution carries out decolorization, and decolorization puts dandelion root segment on glass slide, covers coverslip, observes in root system
The structure features such as Cong Zhi, vesicle and mycelia simultaneously calculate infection rate with root segment observation method；
The concentration of trypan blue described in step 2 is 0.05%, the volume of lactic acid in lactic acid glycerite, glycerol and water
Than for 1:1:1；
From choosing three plants of dandelion seedling in five processed dandelions of stress gradient in step 1, often selects good strains in the field for seed and take phase
With the blade at position, saved backup at a temperature of being subsequently placed in -80 DEG C with liquid nitrogen quick freeze.
Embodiment one, two the utility model has the advantages that
A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi, is inoculated in the soil of cultivation dandelion
Glomus mosseae has turned out the dandelion of inoculation Glomus mosseae, has passed through the gradient provided with 5 NaCl concentrations in experiment
(respectively 0,100mmol/L, 200mmol/L, 300mmol/L, 400mmol/L) is public to the Pu of inoculation Glomus mosseae respectively
English and the dandelion for not being inoculated with Glomus mosseae carry out salt stress test, the results showed that under salt stress, are inoculated with Glomus mosseae
The biomass and plant height for improving dandelion improve the absorption of dandelion internal water and nutrition condition, increase chlorophyll and contain
Measure and improve foliar SOD, POD and CAT activity, at the same reduce MDA content in dandelion body, the absorption of Na, proline contain
The size of amount and relative conductivity, illustrates that Glomus mosseae acts on significantly in terms of promoting dandelion salt resistance ability.
A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi, using potting analog salt stress experiment,
Probe into Glomus mosseae (G.mosseae) to the growth of dandelion under salt stress, moisture absorption, element absorption, physiological metabolism and
The influence of antioxidase, it is intended to the related mechanism that arbuscular mycorrhizal fungi improves salt tolerance of dandelion is disclosed, to be mycorhiza-Pu
Public English syntaxial system repairs salt-affected soil and provides theoretical foundation.
(P < 0.05) is examined by independent sample T, as depicted in figs. 1 and 2:
Fig. 1 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the ground of the dandelion of Glomus mosseae processing
The datagram of top biomass；
Fig. 2 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the root of the dandelion of Glomus mosseae processing
It is the datagram of biomass；
Fig. 1 and Fig. 2 shows that low concentration of salt processing does not generate the growth of dandelion and significantly affects, and high concentration NaCl is coerced
Inhibit the growth of dandelion.Under the salt treatment of all gradients, inoculation Glomus mosseae handles and is not inoculated with Glomus mosseae
It compares, dandelion overground part and root biomass increase.Independent sample T examines (P < 0.05) to show that NaCl concentration exists
0, when 200mmol/L, 400mmol/L concentration, the processing of inoculation Glomus mosseae and the dandelion ground for not being inoculated with Glomus mosseae
Portion and root biomass all have significant difference.Wherein, in 0,100mmol/L, 200mmol/L, 300mmol/L, 400mmol/
Glomus mosseae is inoculated under L concentration to handle so that dandelion the upperground part biomass is not inoculated with Glomus mosseae increasing than control respectively
Added 39.58%, 18.10%, 24.18%, 12.33%, 46.51%, and root biomass increased separately 20.59%,
11.43%, 20.69%, 33.33%, 52.94%.
It is as shown in Figure 3:
Fig. 3 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the strain of the dandelion of Glomus mosseae processing
High datagram；
From the figure 3, it may be seen that salt stress reduces the plant height of dandelion, it is not inoculated in Glomus mosseae processing, in 100mmol/
L, have dropped 1.65% under 200mmol/L, 300mmol/L, 400mmol/L concentration respectively compared with not coercing, 12.57%,
24.42%, 40.60%.Inoculation Glomus mosseae processing increases the plant height of dandelion, wherein 100mmol/L,
It is inoculated between Glomus mosseae handles and be not inoculated with Glomus mosseae and reaches when 200mmol/L, 300mmol/L, 400mmol/L concentration
To the level of signifiance, plant height has increased separately 7.09%, 19.20%, 11.92%, 12.46%, 26.48%.
Using the content of ICP measurement nutrient P, as shown in Figure 4:
Fig. 4 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the P member of the dandelion of Glomus mosseae processing
The datagram of cellulose content；
Phosphorus (P) is a kind of important nutrient in plant, is often inoculated with the life that Glomus mosseae processing promotes plant
Length is attributed to the supply that Glomus mosseae increases P element to plant.The result shows that not being inoculated in Glomus mosseae,
Under 100mmol/L, 200mmol/L, 300mmol/L, 400mmol/L NaCl stress concentration, in dandelion body P element content with
Non- Stress treatment is compared and has dropped 15.65%, 31.30%, 28.12%, 45.51% respectively.It is dense in 0~400mmol/L NaCl
Under degree, inoculation Glomus mosseae processing is so that dandelion overground part P element content is increased than not being inoculated with Glomus mosseae respectively
16.52%, 31.96%, 36.71%, 19.76%, 37.23%.Wherein, in addition to 300mmol/L concentration, it is inoculated with Moses's sacculus
It is mould to handle and be not inoculated with significant difference between Glomus mosseae.(independence sample T is examined, p < 0.05).
Using the proline content of acid ninhydrine method measurement dandelion blade, as shown in Figure 5:
Fig. 5 is be not inoculated with dandelion that Glomus mosseae and inoculation Glomus mosseae are handled auxiliary under five stress gradients
The datagram of histidine content；
Proline is the important osmotic adjustment of plant cell, when being forced, the increase of content and accumulation energy
The osmotic adjustment ability for maintaining cell certain, protects the permeability and enzymatic activity of cell, to improve plant salt endurance.As a result table
Bright, the proline content of the dandelion of inoculation Glomus mosseae processing is below the dandelion for not being inoculated with Glomus mosseae, respectively
9.72%, 20.25%, 9.46%, 22.64%, 16.67% is reduced, wherein in salinity 0,300mmol/L, 400mmol/L
When the processing of inoculation Glomus mosseae be not inoculated with Glomus mosseae and reach significant difference.
Relative conductivity is measured using vacuum suction method, as shown in Figure 6:
Fig. 6 is not to be inoculated with the phase of Glomus mosseae with the dandelion for being inoculated with Glomus mosseae processing under five stress gradients
To the datagram of conductivity；
In plant the variation of relative conductivity can the degree that is hurt of reacting cells film, it is in general, relatively electric
Conductivity value is higher, and the selective penetrated property of plant cell is smaller, therefore the suffered degree coerced of plant is stronger.The result shows that not connecing
In kind Glomus mosseae, compared with salt-free Stress treatment, in addition to dandelion relative conductivity declines under 100mmol/L salinity
In addition, remaining increases as salt stress concentration increases and (has increased separately 21.31%, 46.60% and 85.08%).?
Under same stress concentration, the dandelion relative conductivity of inoculation Glomus mosseae processing, which is below, is not inoculated with processing, reduces respectively
22.65%, 44.12%, 24.85%, 23.98%, 20.66%.
Using the activity of spectrophotometer method measurement superoxide dismutase (SOD), as shown in Figure 7:
Fig. 7 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the super of the dandelion of Glomus mosseae processing
The datagram of superoxide dismutase content；
The result shows that SOD presentation first increases the trend reduced afterwards in dandelion body as salinity increases, it is not inoculated with and rubs
During western sacculus is mould, in addition to 400mmol/L, salt stress increases SOD content in dandelion body.It is inoculated with Glomus mosseae processing
Dandelion SOD content, which is above, is not inoculated with processing.When salinity is 200mmol/L, the Pu of inoculation Glomus mosseae processing is public
SOD activity highest in English body, increases 27.6U/g than not being inoculated with Glomus mosseae at this time.It is inoculated with the Pu of Glomus mosseae processing
Public English SOD activity be significantly higher than in 100mmol/L, 200mmol/L and 400mmol/L be not inoculated with Glomus mosseae (p <
0.05) 1.22,1.24,1.26 times of Glomus mosseae, are not inoculated with respectively.
Using the activity of spectrophotometer method measurement peroxidase (POD), as shown in Figure 8:
Fig. 8 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the mistake of the dandelion of Glomus mosseae processing
The datagram of oxide enzyme content；
The result shows that POD content is higher than not being inoculated with Glomus mosseae in the dandelion body of inoculation Glomus mosseae processing,
And it is not inoculated in 0,200mmol/L, 400mmol/L concentration and between Glomus mosseae with significant difference (independent sample T inspection
It tests, p < 0.05).In inoculation Glomus mosseae processing, with the increase (remove 400mmol/L) of salinity, POD in plant body
Content is higher than non-Stress treatment, shows that dandelion has stronger salt resistance ability, can be in the salt stress environment of high concentration
It is middle to generate a large amount of POD to maintain own growth and development.0,100mmol/L, 200mmol/L, 300mmol/L, 400mmol/L are dense
When spending, inoculation Glomus mosseae handle lower POD content than not being inoculated with Glomus mosseae distinguish high 24.52%, 15.23%,
24.61%, 15.90%, 29.96%.In spite of inoculation Glomus mosseae, POD content in 200mmol/L concentration most
It is high.
The activity of red sanguinin enzyme (CAT) is measured using spectrophotometer method, as shown in Figure 9:
Fig. 9 is be not inoculated with dandelion that Glomus mosseae and inoculation Glomus mosseae are handled red under five stress gradients
The datagram of sanguinin enzyme content；
The result shows that CAT shows the trend for first increasing and reducing afterwards in dandelion body with the increase of salt stress concentration.
Under 0 to 400mmol/L concentration, the ratio of inoculation Glomus mosseae processing is not inoculated with Glomus mosseae and increases separately
27.08%, 18.56%, 25.44%, 38.88%, 49.90%, and it is dense in 200mmol/L, 300mmol/L, 400mmol/L
Significant difference (p < 0.05) is shown when spending.In not being inoculated with Glomus mosseae processing under 400mmol/L concentration in dandelion body
The more non-Stress treatment of CAT content has dropped 51.98%.
Volume fraction is used to measure dandelion leaf chlorophyll content for 95% alcohol extraction, as shown in Figure 10:
Figure 10 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the leaf of the dandelion of Glomus mosseae processing
The datagram of chlorophyll contents；
It is exactly the water inhibited in plant absorption soil that salt stress, which generates a major reason of injury to the growth of plant,
Divide and reduce the photosynthesis of plant.It can be reflected by measurement chlorophyll content of plant at inoculation Glomus mosseae
Manage the response to salt stress.The result shows that the Pu for not being inoculated with Glomus mosseae is public under 100mmol/L, 200mmol/L concentration
The chlorophyll content of English with no salt stress processing compare no significant changes, and work as NaCl concentration 300mmol/L,
When 400mmol/L, chlorophyll content, which sharply declines, (declines low 19.30%, 28.95%) than non-salt stress respectively.Dandelion connects
After the processing of kind of Glomus mosseae, chlorophyll content increases, increase by 19.03% than not being inoculated with Glomus mosseae respectively, 2.53%,
11.06%, 25.25%, 27.55%, independent sample T examine display, in 0,200mmol/L, 400mmol/L, are inoculated with Moses
Sacculus is mould and is not inoculated with Glomus mosseae significant difference.
Using gravimetric detemination tissue water content, as shown in figure 11:
Figure 11 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the group of the dandelion of Glomus mosseae processing
Knit the datagram of water content；
The result shows that not being inoculated with Glomus mosseae under 100mmol/L, 200mmol/L, 300mmol/LNaCl concentration
The tissue water content no significant change compared under non-stress for handling lower dandelion blade, when NaCl concentration is in 400mmol/L
When, dandelion plant leaf tissue water content is decreased obviously, and has dropped 26.07%.Under 0~400mmol/L NaCl concentration,
Except the dandelion tissue water content of 100mmol/L concentration inoculation Glomus mosseae processing slightly declines, remaining concentration dandelion body
Inner tissue's water content has increased separately 20.52%, 26.87%, 28.19%, 40.99% than not being inoculated with Glomus mosseae processing.
Using the content of thiobarbituricacidα- method measurement malonaldehyde, as shown in figure 12:
Figure 12 is not to be inoculated with the third of the dandelion that Glomus mosseae and inoculation Glomus mosseae are handled under five stress gradients
The datagram of dialdehyde content；
The result shows that lipid peroxidation is the index for judging Plants under Salt Stress plasma membrane and permeating the extent of damage, malonaldehyde
(MDA) it is commonly used to measure Membrane-Lipid Peroxidation in Plants degree.Under potting processing, in 100mmol/L salinity, and phase is not coerced
Than MDA content reduces 18.75%.Later, with the increase of salinity, the MDA content of dandelion increases therewith (to be increased respectively
Added 37.5%, 56.25,106.25).Compared with not being inoculated with Glomus mosseae processing, inoculation Glomus mosseae reduces Pu public affairs
English-Chinese mda content reduces 37.50%, 7.69%, 22.73%, 40.74%, 33.33% respectively.Wherein, it removes
Outside 100mmol/L, remaining concentration difference is significant (p < 0.05).
Using the content of ICP measurement Na, as shown in figure 13:
Figure 13 is not to be inoculated with Glomus mosseae under five stress gradients and be inoculated with the Na of the dandelion of Glomus mosseae processing
The datagram of constituent content；
The result shows that regardless of whether inoculation Glomus mosseae, with the increase of salt stress concentration, sodium ion in dandelion body
Content increases.Inoculation Glomus mosseae reduces the content of Na in dandelion body, reduce 23.63% respectively, 26.74%,
6.92%, 27.15%, 28.13%.In 100mmol/L, 300mmol/L, 400mmol/L concentration, it is inoculated with Glomus mosseae
Handle and be not inoculated with significant difference between Glomus mosseae processing.In 400mmol/L concentration, it is not inoculated with Glomus mosseae processing
Dandelion body in Na content be no salt stress processing when 3.65 times.
Claims (10)
1. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability, it is characterised in that this method is according to the following steps
It completes:
One, inoculation handle: will in several flowerpots first be added 130 parts~140 parts soil, then by 260 parts~280 parts soil with
28 parts~32 parts arbuscular mycorrhizal fungi are stirring evenly and then adding into each flowerpot；
Two, Taraxacum mongolicum seeds are sowed: Taraxacum mongolicum seeds being sowed into step 1 in each flowerpot, and adjacent Taraxacum mongolicum seeds
Spacing is identical, then upper 20 parts~30 parts soil that tile in each flowerpot；
Three, Taraxacum mongolicum seeds culture: the flowerpot of sowing Taraxacum mongolicum seeds all in step 2 is placed in culturing room and is cultivated, is cultivated
Environment: light application time is 12h/d~16h/d, and intensity of illumination is 4800lx~5200lx, and temperature is 23 DEG C~25 DEG C, air phase
It is 60%~70% to humidity, and keeping the water content of soil in each flowerpot is 55%~70%.
2. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is that the soil is Nutrition Soil, Nutrition Soil is made of river sand, vermiculite and garden mould, the mass ratio of river sand, vermiculite and garden mould
For 1:1:3.
3. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is soil described in step 1 before use, 2h~the 2.5h that sterilizes under the conditions of 121 DEG C~122 DEG C of temperature.
4. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is that the bottom of flowerpot described in step 1 has permeable hole.
5. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is in step 1 for 260 parts~280 parts soil and 30 parts of arbuscular mycorrhizal fungi to be stirring evenly and then adding into each flowerpot.
6. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is that arbuscular mycorrhizal fungi described in step 1 is Glomus mosseae.
7. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is in step 2 before Taraxacum mongolicum seeds sowing, is placed in the KMnO that mass fraction is 0.2%~0.4%428min is sterilized in solution
~32min.
8. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is in step 2 that each flowerpot sows 10 Taraxacum mongolicum seeds.
9. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is in step 2 the upper 25 parts of soil that tiles in each flowerpot.
10. a kind of method using arbuscular mycorrhizal fungi enhancing dandelion salt resistance ability according to claim 1, feature
It is that culture environment in step 3: light application time 14h/d, intensity of illumination 5000lx, temperature are 24 DEG C, relative air humidity
It is 65%, and keeping the water content of soil in each flowerpot is 60%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910502087.5A CN110122208A (en) | 2019-06-11 | 2019-06-11 | A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910502087.5A CN110122208A (en) | 2019-06-11 | 2019-06-11 | A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110122208A true CN110122208A (en) | 2019-08-16 |
Family
ID=67581018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910502087.5A Pending CN110122208A (en) | 2019-06-11 | 2019-06-11 | A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110122208A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111117640A (en) * | 2019-12-24 | 2020-05-08 | 鞍钢集团矿业有限公司 | Saline-alkali soil improver containing glomus mosseae and improvement method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285621A (en) * | 2014-08-14 | 2015-01-21 | 亳州市凯利中药饮片有限公司 | Breeding method of dual-purpose dandelions for medicine and food |
| CN108156874A (en) * | 2017-12-28 | 2018-06-15 | 潍坊友容实业有限公司 | Plant naringi crenulata and the method for improveing clay fertilizer situation in strand sandyization salt-soda soil |
-
2019
- 2019-06-11 CN CN201910502087.5A patent/CN110122208A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285621A (en) * | 2014-08-14 | 2015-01-21 | 亳州市凯利中药饮片有限公司 | Breeding method of dual-purpose dandelions for medicine and food |
| CN108156874A (en) * | 2017-12-28 | 2018-06-15 | 潍坊友容实业有限公司 | Plant naringi crenulata and the method for improveing clay fertilizer situation in strand sandyization salt-soda soil |
Non-Patent Citations (3)
| Title |
|---|
| 岳英男: ""松嫩盐碱草地主要丛枝菌根真菌对植物耐盐性影响的研究"", 《东北林业大学学位论文》 * |
| 张爱娣等: ""丛枝菌根真菌对大叶女贞耐盐性的影响"", 《江苏农业科学》 * |
| 高璿濛等: ""松嫩盐碱草地蒲公英根围ＡＭ 真菌侵染特性及种类多样性"", 《中国农业大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111117640A (en) * | 2019-12-24 | 2020-05-08 | 鞍钢集团矿业有限公司 | Saline-alkali soil improver containing glomus mosseae and improvement method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101845409B (en) | Bacillus firmus and application thereof | |
| CN105296381A (en) | Bacillus subtilis CYY-25 and application thereof | |
| CN104726378B (en) | The method for improving salt stress turfgrass defence enzyme activity using Salt-tolerant microbial agent is strengthened | |
| CN103103152B (en) | Corallococcus coralloides and application thereof | |
| CN104542714B (en) | Rhizobium Sinorhizobium meliloti CCNWSX0020 application | |
| Yin et al. | Effects of an ectomycorrhizal fungus on the growth and physiology of Pinus sylvestris var. mongolica seedlings subjected to saline–alkali stress | |
| Zou et al. | Mycorrhizal and non-mycorrhizal responses to salt stress in trifoliate orange: plant growth, root architecture and soluble sugar accumulation | |
| CN103937720B (en) | The nematicide albumen of one strain wax printing fabric and secretion thereof | |
| CN104789483A (en) | Method for isolated culture of endoclyta signifer walker beauveria bassiana (bals.) vuill strain, and prevention and control of endoclyta signifer walkers | |
| CN104756754B (en) | A kind of cultural method of Antrodia camphorata fructification | |
| KR100483333B1 (en) | Production method of the cauliflower mushroom using fermented sawdust | |
| CN107858292A (en) | A kind of Antrodia camphorata bacterial strain and its method for preserving | |
| CN108913625A (en) | Salt tolerant streptomycete, its microbial inoculum and its microbial inoculum are promoting the application in plant growth | |
| CN110122208A (en) | A method of enhancing dandelion salt resistance ability using arbuscular mycorrhizal fungi | |
| CN103340091B (en) | Method for artificially cultivating xuefengensis | |
| Yuan et al. | Extensive host range of an endophytic fungus affects the growth and physiological functions in rice (Oryza sativa L.) | |
| CN102577806B (en) | Method for using Bacillus thruingiensis fermentation broth to improve salt resistance of lawn plants | |
| CN105580688B (en) | A method of honeysuckle Cd accumulation is reduced using Glomus mosseae and improves its yield | |
| CN108277167A (en) | A kind of method of biological control Phytophthora capsici disease | |
| CN104920066B (en) | The method for improving host of Cordyceps sinensis infection rate | |
| CN104604500B (en) | A kind of raising Semen sojae atricolor is in the method for salt-soda soil adaptability | |
| Ajiboye et al. | Effect of presowing treatments on seed germination and percentage starch content levels in Tamarindus indica, prosopis africana, parkia biglobossa and Albizia lebbeck | |
| CN104798819B (en) | A kind of method for improving turfgrass anti-seismic design using low temperature resistant microbial bacterial agent is strengthened | |
| CN104818219B (en) | One plant of endogenetic fungus for promoting aleurite montana root growth under low-phosphorous environment | |
| CN102598980B (en) | Method for using Bacillus thruingiensis fermentation liquor to adjust protective enzymes in Festuca arundinacea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190816 |
|
| RJ01 | Rejection of invention patent application after publication |