CN114557348A

CN114557348A - Sex attractant for bactrocera dorsalis and application thereof

Info

Publication number: CN114557348A
Application number: CN202210221868.9A
Authority: CN
Inventors: 赵明辉; 董雪云; 王利; 李慧
Original assignee: Harbin University
Current assignee: Harbin University
Priority date: 2022-03-07
Filing date: 2022-03-07
Publication date: 2022-05-31
Anticipated expiration: 2042-03-07
Also published as: CN114557348B

Abstract

The invention discloses a citrus fruit fly sex attractant and application thereof, belonging to the technical field of insect sex pheromone preparation, wherein the sex attractant comprises N- (3-methylbutyl) propionamide, N- (3-methylbutyl) acetamide and N-2-methylbutyl acetamide, and the mass ratio of the N- (3-methylbutyl) propionamide to the N- (3-methylbutyl) acetamide is 14:3: 3. the citrus fruit fly sex attractant provided by the invention can efficiently attract female citrus fruit fly, reduce the mating probability of female and male citrus fruit fly and reduce the harm; green and safe, and no pollution residue; the lasting period is long, and the female insects can be trapped and killed for a long time. The sex attractant can be applied to insect condition monitoring, mass trapping and mating interference of bactrocera dorsalis, and has important significance for green prevention and control of bactrocera dorsalis.

Description

Sex attractant for bactrocera dorsalis and application thereof

Technical Field

The invention relates to the technical field of insect sex pheromone preparation, in particular to a bactrocera dorsalis sex attractant and application thereof.

Background

Bactrocera dorsalis Hendel belongs to Diptera, and is called as oriental fruit fly, and is mainly distributed in provinces such as Guangdong, Guangxi, Hunan, Guizhou, Fujian and the like at home, and 250 fruits and vegetables of 46 families such as banana, orange, carambola, guava, mango, eggplant, hot pepper and the like are taken. The female adults lay eggs in the host fruits, the egg laying amount of each female is 400-. The method has the characteristics of wide host range, hidden harm, large reproduction quantity and the like, is classified as quarantine pests in many countries and regions, can quickly spread the harm when meeting a suitable growth environment, and poses serious threat to the development of the vegetable industry and the flower industry.

At present, the methyl eugenol and the insecticidal lamp are used in combination in the field to prevent and control the bactrocera dorsalis, the defects of large using amount of the methyl eugenol, high preventing and controlling cost, strong volatility, short using time of trapping cores and the like exist in the preventing and controlling process, and the methyl eugenol is only used for trapping and killing male insects but not for trapping and killing female insects. Therefore, a technology for green, efficient and comprehensive control of bactrocera dorsalis is urgently needed. The sex pheromone generated by the male citrus fruit fly has strong attraction to the same species of female insects, not only can trap a large number of pests, but also achieves the purpose of monitoring the pests, and has the characteristics of trace quantity, high efficiency, green safety and the like.

Disclosure of Invention

The invention aims to provide a bactrocera dorsalis sex attractant and application thereof, so as to achieve the purpose of green prevention and control of bactrocera dorsalis.

In order to achieve the purpose, the following technical scheme is adopted:

a sex attractant for bactrocera dorsalis comprises N- (3-methylbutyl) propionamide, N- (3-methylbutyl) acetamide and N-2-methylbutyl acetamide.

Optionally, the mass ratio of N- (3-methylbutyl) propionamide, N- (3-methylbutyl) acetamide and N-2-methylbutyl acetamide in the bactrocera dorsalis sex attractant is (10-20): (1-4): (1-4).

Preferably, the mass ratio of the N- (3-methylbutyl) propionamide, the N- (3-methylbutyl) acetamide and the N-2-methylbutyl acetamide in the bactrocera dorsalis sex attractant is (12-16): (2-3): (2-3).

Preferably, the bactrocera dorsalis sex attractant comprises N- (3-methylbutyl) propionamide, N- (3-methylbutyl) acetamide and N-2-methylbutyl acetamide in a mass ratio of 14:3: 3.

the citrus fruit fly sex attractant comprises N- (3-methylbutyl) propionamide with the mass of 500-1000 mu g, N- (3-methylbutyl) acetamide with the mass of 50-200 mu g, and N-2-methylbutyl acetamide with the mass of 50-200 mu g.

The citrus fruit fly sex attractant can be made into a sex attractant attracting core, and the trapper fixed with the citrus fruit fly sex attractant attracting core is hung in an area where citrus fruit flies happen or possibly happen, so that the trapper is used for trapping citrus fruit flies in a large quantity, interfering mating and monitoring insect situations.

The preparation method of the sex attractant lure comprises the following steps: injecting n-hexane solution containing the bactrocera dorsalis sex attractant into a black polyethylene bottle to obtain a lure core containing the bactrocera dorsalis sex attractant, packaging the lure core into aluminum foil, sealing, and refrigerating at-20 deg.C.

Compared with the prior art, the invention has the following beneficial effects: the citrus fruit fly sex attractant provided by the invention can efficiently attract female citrus fruit fly, reduce the mating probability of female and male citrus fruit fly and reduce the harm; green and safe, and no pollution residue; the lasting period is long, and the female insects can be trapped and killed for a long time. The sex attractant can be applied to trapping a large number of bactrocera dorsalis, disturbing mating and monitoring insect conditions, and has important significance for green prevention and control of the bactrocera dorsalis.

Drawings

FIG. 1 is a graph of the average number of female traps for different lure formulations, where different letters indicate the number of female traps at the 0.05 level.

FIG. 2 is a graph of mean female trap number for different lure densities, with different letters indicating female trap number at the 0.05 level.

Detailed Description

The present invention is described in detail below with reference to the drawings and examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.

Example 1 screening of optimal formulation of sex attractant for bactrocera dorsalis

And injecting the normal hexane solution containing the bactrocera dorsalis sex attractant into a black polyethylene bottle to prepare the lure containing the bactrocera dorsalis sex attractant. The lure was placed in a barrel-shaped trap and then the trap was placed in an orchard where bactrocera dorsalis is more severe. In order to ensure the release effect, 150-1400 mu g of bactrocera dorsalis sex attractant is added into a single black polyethylene bottle,

the average spacing between traps was 10m, the lure height was set to 1.5m from the ground, and the trap type was a barrel trap. To further illustrate the feasibility of this example, 12 specific formulations are given in table 1.

TABLE 1

Each formula was set up in 3 replicates, the number of trapped bactrocera dorsalis flies was investigated every two days after placement, the trapping sites were randomly changed, and the investigation was continued for 30 days, with the results shown in table 2.

TABLE 2

Formulation of	Total trapping volume (head)
		1	0
2	5
		3	0
4	6
		5	11
6	5
		7	5
8	435
		9	69
10	88
		11	71
12	86

The data in the table are counted and made into figure 1, and as can be seen from figure 1, the bactrocera dorsalis sex attractant has a good trapping effect on bactrocera dorsalis females. As is clear from the comparison of the significance of difference (P < 0.05), the maximum amount of female insects trapped in formulation No. 8 was observed when 700. mu.g of N- (3-methylbutyl) propionamide, 150. mu.g of N- (3-methylbutyl) acetamide, and 150. mu.g of N-2-methylbutyl acetamide were added. The trapping effect is best when the mass ratio of the N- (3-methylbutyl) propionamide to the N- (3-methylbutyl) acetamide to the N-2-methylbutyl acetamide is 14:3:3 and the amount of the lure additive is 1000 mu g. Comparing formula 2, formula 3, formula 4, formula 5, formula 6, formula 7 and formula 8, it is demonstrated that sex pheromone produced by male citrus fruit fly can only attract female insect when they exist at the same time. Comparing formula 8 with formula 9, formula 10, formula 11, and formula 12, it is shown that differences in the mixture ratios of the sex pheromone components can lead to differences in trapping amounts, and that the specific sex pheromone components and ratios ensure specificity of the trapped pests without harming other beneficial species.

Example 2 optimal trapping density in a field screen of sexual attractants for bactrocera dorsalis

The lure was prepared using formulation 8 in example 1, and a garden in the Yanqing area where bactrocera dorsalis occurred was selected, the area was over 10 mu, the height of the lure was 1.5m, the type of the lure was a bucket type, 4 different lure distances were set, and 3 replicates were set for each treatment.

TABLE 3

Treatment of	Lure distance (rice)
		1	5
2	10
		3	15
4	20

The number of the fruit flies trapped was investigated every two days after the placement, and the trapping sites were randomly changed for 30 days, and the results of the investigation are shown in Table 4.

TABLE 4

Categories	Total trapping volume (head)
		1	167
2	476
		3	337
4	215

As shown in FIG. 2, the data in Table 4 were counted and shown in FIG. 2, when the distance between the lure was 10m, the attracting effect was more desirable, and the average trapping amount was 158.67. + -. 11.29. Compared with other treatments, the attraction effect is not ideal when the attraction cores are too close or too far.

The above disclosure is only for the specific embodiment of the present invention, but the present invention is not limited thereto, and any variations that can be made by those skilled in the art should fall within the scope of the present invention.

Claims

1. The sex attractant for the bactrocera dorsalis hendel is characterized by comprising N- (3-methylbutyl) propionamide, N- (3-methylbutyl) acetamide and N-2-methylbutyl acetamide.

2. The citrus fruit fly sex attractant as claimed in claim 1, wherein the mass ratio of N- (3-methylbutyl) propionamide, N- (3-methylbutyl) acetamide and N-2-methylbutyl acetamide in the citrus fruit fly sex attractant is (10-20): (1-4): (1-4).

3. The citrus fruit fly sex attractant according to claim 1, wherein the mass ratio of the N- (3-methylbutyl) propionamide, the N- (3-methylbutyl) acetamide and the N-2-methylbutyl acetamide in the citrus fruit fly sex attractant is (12-16): (2-3): (2-3).

4. The citrus fruit fly sex attractant as claimed in claim 1, wherein the mass ratio of N- (3-methylbutyl) propionamide, N- (3-methylbutyl) acetamide and N-2-methylbutyl acetamide in the citrus fruit fly sex attractant is 14:3: 3.

5. the sex attractant for the bactrocera dorsalis according to claim 1, wherein the mass of the N- (3-methylbutyl) propionamide in the sex attractant for the bactrocera dorsalis is 500-1000 μ g, the mass of the N- (3-methylbutyl) acetamide is 50-200 μ g, and the mass of the N-2-methylbutyl acetamide is 50-200 μ g.

6. The use of a citrus fruit fly sex attractant according to claim 1, wherein the manufactured citrus fruit fly sex attractant lure is fixed in a trap and the trap is suspended in an orchard where citrus fruit flies occur or are likely to occur, for the purpose of mass trapping of citrus fruit flies, disturbing mating and insect condition monitoring.

7. The use of the sexual attractant for bactrocera dorsalis according to claim 1, wherein the sexual attractant for bactrocera dorsalis is made into a sexual attractant attracting core, and the trap to which the sexual attractant attracting core for bactrocera dorsalis is fixed is hung in an area where bactrocera dorsalis occurs or is likely to occur, so that the sexual attractant for bactrocera dorsalis can be used for trapping bactrocera dorsalis in a large quantity, interfering with mating and monitoring insect pest situations.

8. The use of the sex attractant for bactrocera dorsalis according to claim 7, wherein the sex attractant core is prepared by the following steps: injecting normal hexane solution containing the bactrocera dorsalis sex attractant into a black polyethylene bottle, sealing to obtain the lure core containing the bactrocera dorsalis sex attractant, packaging the lure core into aluminum foil, sealing, and refrigerating at-20 deg.C.