CN114556105A - 利用血中外泌体的蛋白质标志物的covid-19重症化预测方法 - Google Patents
利用血中外泌体的蛋白质标志物的covid-19重症化预测方法 Download PDFInfo
- Publication number
- CN114556105A CN114556105A CN202180002820.2A CN202180002820A CN114556105A CN 114556105 A CN114556105 A CN 114556105A CN 202180002820 A CN202180002820 A CN 202180002820A CN 114556105 A CN114556105 A CN 114556105A
- Authority
- CN
- China
- Prior art keywords
- protein
- level
- marker
- patient
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 137
- 208000025721 COVID-19 Diseases 0.000 title claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 title claims description 242
- 102000004169 proteins and genes Human genes 0.000 title claims description 239
- 210000001808 exosome Anatomy 0.000 title claims description 93
- 210000004369 blood Anatomy 0.000 title claims description 85
- 239000008280 blood Substances 0.000 title claims description 85
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims abstract description 109
- 102100024783 Fibrinogen gamma chain Human genes 0.000 claims abstract description 41
- 102100032412 Basigin Human genes 0.000 claims abstract description 38
- 101000798441 Homo sapiens Basigin Proteins 0.000 claims abstract description 38
- 102100031758 Extracellular matrix protein 1 Human genes 0.000 claims abstract description 37
- 102100024923 Protein kinase C beta type Human genes 0.000 claims abstract description 36
- 102100022589 Coatomer subunit beta' Human genes 0.000 claims abstract description 35
- 101000899916 Homo sapiens Coatomer subunit beta' Proteins 0.000 claims abstract description 35
- 101001051767 Homo sapiens Protein kinase C beta type Proteins 0.000 claims abstract description 35
- 101000947699 Homo sapiens Microfibril-associated glycoprotein 4 Proteins 0.000 claims abstract description 30
- 102100036103 Microfibril-associated glycoprotein 4 Human genes 0.000 claims abstract description 30
- 102100031752 Fibrinogen alpha chain Human genes 0.000 claims abstract description 17
- 102100028313 Fibrinogen beta chain Human genes 0.000 claims abstract description 17
- 102100040681 Platelet-derived growth factor C Human genes 0.000 claims abstract description 16
- 102100027610 Rho-related GTP-binding protein RhoC Human genes 0.000 claims abstract description 14
- 102100022622 Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Human genes 0.000 claims abstract description 6
- 102100030708 GTPase KRas Human genes 0.000 claims abstract 12
- 101001052043 Homo sapiens Fibrinogen gamma chain Proteins 0.000 claims abstract 12
- 101000584612 Homo sapiens GTPase KRas Proteins 0.000 claims abstract 12
- 101000581118 Homo sapiens Rho-related GTP-binding protein RhoC Proteins 0.000 claims abstract 12
- 102100032537 Calpain-2 catalytic subunit Human genes 0.000 claims abstract 8
- 101000867692 Homo sapiens Calpain-2 catalytic subunit Proteins 0.000 claims abstract 8
- 101000866526 Homo sapiens Extracellular matrix protein 1 Proteins 0.000 claims abstract 8
- 102100021834 3-hydroxyacyl-CoA dehydrogenase Human genes 0.000 claims abstract 5
- 101000611888 Homo sapiens Platelet-derived growth factor C Proteins 0.000 claims abstract 5
- 101710089435 Lipopolysaccharide-binding protein Proteins 0.000 claims abstract 5
- 102100031051 Cysteine and glycine-rich protein 1 Human genes 0.000 claims abstract 2
- 108091006374 cAMP receptor proteins Proteins 0.000 claims abstract 2
- 239000003550 marker Substances 0.000 claims description 186
- 230000027455 binding Effects 0.000 claims description 83
- -1 CLDN Proteins 0.000 claims description 75
- 239000000126 substance Substances 0.000 claims description 62
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 46
- 201000010099 disease Diseases 0.000 claims description 45
- 239000000203 mixture Substances 0.000 claims description 32
- 208000015181 infectious disease Diseases 0.000 claims description 23
- 208000024891 symptom Diseases 0.000 claims description 23
- 239000012634 fragment Substances 0.000 claims description 21
- 230000000391 smoking effect Effects 0.000 claims description 15
- 102100032126 Aminopeptidase B Human genes 0.000 claims description 14
- 102100021408 14-3-3 protein beta/alpha Human genes 0.000 claims description 12
- 102100035923 4-aminobutyrate aminotransferase, mitochondrial Human genes 0.000 claims description 12
- 102100020775 Adenylosuccinate lyase Human genes 0.000 claims description 12
- 102100040202 Apolipoprotein B-100 Human genes 0.000 claims description 12
- 102100025991 Betaine-homocysteine S-methyltransferase 1 Human genes 0.000 claims description 12
- 102100038077 CD226 antigen Human genes 0.000 claims description 12
- 101000818893 Homo sapiens 14-3-3 protein beta/alpha Proteins 0.000 claims description 12
- 101001000686 Homo sapiens 4-aminobutyrate aminotransferase, mitochondrial Proteins 0.000 claims description 12
- 101000775829 Homo sapiens Aminopeptidase B Proteins 0.000 claims description 12
- 101000889953 Homo sapiens Apolipoprotein B-100 Proteins 0.000 claims description 12
- 101000933413 Homo sapiens Betaine-homocysteine S-methyltransferase 1 Proteins 0.000 claims description 12
- 101000884298 Homo sapiens CD226 antigen Proteins 0.000 claims description 12
- 101000959666 Homo sapiens Eukaryotic initiation factor 4A-I Proteins 0.000 claims description 12
- 239000000427 antigen Substances 0.000 claims description 12
- 108091007433 antigens Proteins 0.000 claims description 12
- 102000036639 antigens Human genes 0.000 claims description 12
- 230000005713 exacerbation Effects 0.000 claims description 12
- WWJZWCUNLNYYAU-UHFFFAOYSA-N temephos Chemical compound C1=CC(OP(=S)(OC)OC)=CC=C1SC1=CC=C(OP(=S)(OC)OC)C=C1 WWJZWCUNLNYYAU-UHFFFAOYSA-N 0.000 claims description 12
- 102100020999 Argininosuccinate synthase Human genes 0.000 claims description 11
- 102100039874 Guanine nucleotide-binding protein G(z) subunit alpha Human genes 0.000 claims description 11
- 101000887490 Homo sapiens Guanine nucleotide-binding protein G(z) subunit alpha Proteins 0.000 claims description 11
- 108091051828 miR-122 stem-loop Proteins 0.000 claims description 11
- 108091066112 miR-122-1 stem-loop Proteins 0.000 claims description 11
- 108091057488 miR-122-2 stem-loop Proteins 0.000 claims description 11
- 108091008064 CDKN2B-AS1 Proteins 0.000 claims description 10
- 108700040193 Adenylosuccinate lyases Proteins 0.000 claims description 9
- 108700040066 Argininosuccinate lyases Proteins 0.000 claims description 9
- 101150025804 Asl gene Proteins 0.000 claims description 9
- 102100022118 Leukotriene A-4 hydrolase Human genes 0.000 claims description 9
- 108091073704 let-7c stem-loop Proteins 0.000 claims description 4
- 108091081439 let-7c-1 stem-loop Proteins 0.000 claims description 4
- 108091062190 let-7c-2 stem-loop Proteins 0.000 claims description 4
- 108091064407 let-7c-3 stem-loop Proteins 0.000 claims description 4
- 108091060382 miR-140 stem-loop Proteins 0.000 claims description 4
- 108091030617 miR-140-1 stem-loop Proteins 0.000 claims description 4
- 108091023370 miR-140-2 stem-loop Proteins 0.000 claims description 4
- 108091062762 miR-21 stem-loop Proteins 0.000 claims description 4
- 208000028399 Critical Illness Diseases 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 102100024365 Arf-GAP domain and FG repeat-containing protein 1 Human genes 0.000 claims 8
- 101000833314 Homo sapiens Arf-GAP domain and FG repeat-containing protein 1 Proteins 0.000 claims 5
- 101000746496 Schizosaccharomyces pombe (strain 972 / ATCC 24843) GTP-binding protein ypt3 Proteins 0.000 claims 5
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 claims 4
- JJBCTCGUOQYZHK-UHFFFAOYSA-N 2-acetyloxybenzoate;(5-amino-1-carboxypentyl)azanium Chemical compound OC(=O)C(N)CCCC[NH3+].CC(=O)OC1=CC=CC=C1C([O-])=O JJBCTCGUOQYZHK-UHFFFAOYSA-N 0.000 claims 4
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 claims 4
- 102100030972 Coatomer subunit beta Human genes 0.000 claims 4
- 102100022462 Eukaryotic initiation factor 4A-II Human genes 0.000 claims 4
- 102000001267 GSK3 Human genes 0.000 claims 4
- 108060006662 GSK3 Proteins 0.000 claims 4
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 claims 4
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 claims 4
- 101000972916 Homo sapiens Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Proteins 0.000 claims 4
- 101000919970 Homo sapiens Coatomer subunit beta Proteins 0.000 claims 4
- 101001044475 Homo sapiens Eukaryotic initiation factor 4A-II Proteins 0.000 claims 4
- 101001112229 Homo sapiens Neutrophil cytosol factor 1 Proteins 0.000 claims 4
- 102100023620 Neutrophil cytosol factor 1 Human genes 0.000 claims 4
- 102100022851 Rab5 GDP/GTP exchange factor Human genes 0.000 claims 4
- 101710203837 Replication-associated protein Proteins 0.000 claims 4
- 101000930003 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Diacylglycerol O-acyltransferase 1 Proteins 0.000 claims 4
- 101001014956 Schistosoma mansoni Glutathione peroxidase Proteins 0.000 claims 4
- NFVJNJQRWPQVOA-UHFFFAOYSA-N n-[2-chloro-5-(trifluoromethyl)phenyl]-2-[3-(4-ethyl-5-ethylsulfanyl-1,2,4-triazol-3-yl)piperidin-1-yl]acetamide Chemical compound CCN1C(SCC)=NN=C1C1CN(CC(=O)NC=2C(=CC=C(C=2)C(F)(F)F)Cl)CCC1 NFVJNJQRWPQVOA-UHFFFAOYSA-N 0.000 claims 4
- 101100168911 Arabidopsis thaliana CUL4 gene Proteins 0.000 claims 3
- 102100026678 Carboxypeptidase N catalytic chain Human genes 0.000 claims 3
- 101710147840 Carboxypeptidase N catalytic chain Proteins 0.000 claims 3
- 101100168913 Dictyostelium discoideum culD gene Proteins 0.000 claims 3
- 101150070251 MAN1A1 gene Proteins 0.000 claims 3
- 101100028435 Mus musculus Pacsin1 gene Proteins 0.000 claims 3
- 101100168914 Schizosaccharomyces pombe (strain 972 / ATCC 24843) pcu4 gene Proteins 0.000 claims 3
- 102100025518 Carbonic anhydrase 1 Human genes 0.000 claims 2
- 102100032363 Choline dehydrogenase, mitochondrial Human genes 0.000 claims 2
- 101001132698 Homo sapiens Retinoic acid receptor beta Proteins 0.000 claims 2
- 102100033909 Retinoic acid receptor beta Human genes 0.000 claims 2
- 101100108866 Callithrix jacchus AGT gene Proteins 0.000 claims 1
- 101000936262 Homo sapiens ATP synthase subunit alpha, mitochondrial Proteins 0.000 claims 1
- 101001078133 Homo sapiens Integrin alpha-2 Proteins 0.000 claims 1
- 101100455054 Homo sapiens LTA4H gene Proteins 0.000 claims 1
- 101001068219 Homo sapiens Serine/threonine-protein phosphatase 4 catalytic subunit Proteins 0.000 claims 1
- 102100025305 Integrin alpha-2 Human genes 0.000 claims 1
- 101100075025 Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) LTA4 gene Proteins 0.000 claims 1
- 102100034492 Serine/threonine-protein phosphatase 4 catalytic subunit Human genes 0.000 claims 1
- 102100036049 T-complex protein 1 subunit gamma Human genes 0.000 claims 1
- 101150062912 cct3 gene Proteins 0.000 claims 1
- 238000004989 laser desorption mass spectroscopy Methods 0.000 claims 1
- UFPQIRYSPUYQHK-WAQVJNLQSA-N leukotriene A4 Chemical compound CCCCC\C=C/C\C=C/C=C/C=C/[C@@H]1O[C@H]1CCCC(O)=O UFPQIRYSPUYQHK-WAQVJNLQSA-N 0.000 claims 1
- 102100040605 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase Human genes 0.000 abstract description 20
- 101000966793 Homo sapiens 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase Proteins 0.000 abstract description 15
- 102100038664 Fibrinogen-like protein 1 Human genes 0.000 abstract description 13
- 108010051975 Glycogen Synthase Kinase 3 beta Proteins 0.000 abstract description 13
- 102100022303 Ras-related protein Rab-13 Human genes 0.000 abstract description 13
- 102100030705 Ras-related protein Rap-1b Human genes 0.000 abstract description 13
- 108010012996 Serotonin Plasma Membrane Transport Proteins Proteins 0.000 abstract description 13
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract description 12
- 102100039441 Cytochrome b-c1 complex subunit 2, mitochondrial Human genes 0.000 abstract description 12
- 102100033039 Glutathione peroxidase 1 Human genes 0.000 abstract description 12
- 102100037938 Ubiquitin-like modifier-activating enzyme 7 Human genes 0.000 abstract description 12
- 102100027236 Adenylate kinase isoenzyme 1 Human genes 0.000 abstract description 11
- 102100038423 Claudin-3 Human genes 0.000 abstract description 11
- 101000882908 Homo sapiens Claudin-3 Proteins 0.000 abstract description 11
- 101000746756 Homo sapiens Cytochrome b-c1 complex subunit 2, mitochondrial Proteins 0.000 abstract description 11
- 101000846244 Homo sapiens Fibrinogen alpha chain Proteins 0.000 abstract description 11
- 101000917163 Homo sapiens Fibrinogen beta chain Proteins 0.000 abstract description 11
- 101000620529 Homo sapiens Ras-related protein Rab-13 Proteins 0.000 abstract description 11
- 101000584600 Homo sapiens Ras-related protein Rap-1b Proteins 0.000 abstract description 11
- 102000005038 SLC6A4 Human genes 0.000 abstract description 11
- 208000037847 SARS-CoV-2-infection Diseases 0.000 abstract description 5
- 101710186714 2-acylglycerol O-acyltransferase 1 Proteins 0.000 abstract 1
- 101000779375 Dictyostelium discoideum Alpha-protein kinase 1 Proteins 0.000 abstract 1
- 102000019058 Glycogen Synthase Kinase 3 beta Human genes 0.000 abstract 1
- 101001057251 Homo sapiens Adenylate kinase isoenzyme 1 Proteins 0.000 abstract 1
- 101001031635 Homo sapiens Fibrinogen-like protein 1 Proteins 0.000 abstract 1
- 101001014936 Homo sapiens Glutathione peroxidase 1 Proteins 0.000 abstract 1
- 101000662026 Homo sapiens Ubiquitin-like modifier-activating enzyme 7 Proteins 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 212
- 239000000523 sample Substances 0.000 description 58
- 238000004458 analytical method Methods 0.000 description 45
- 241000711573 Coronaviridae Species 0.000 description 41
- 238000007689 inspection Methods 0.000 description 41
- 101710127949 Extracellular matrix protein 1 Proteins 0.000 description 29
- 210000001124 body fluid Anatomy 0.000 description 29
- 108010048325 fibrinopeptides gamma Proteins 0.000 description 29
- 150000007523 nucleic acids Chemical class 0.000 description 29
- 102000003900 Calpain-2 Human genes 0.000 description 28
- 108090000232 Calpain-2 Proteins 0.000 description 28
- 102000039446 nucleic acids Human genes 0.000 description 28
- 108020004707 nucleic acids Proteins 0.000 description 28
- 239000010839 body fluid Substances 0.000 description 26
- 108010074051 C-Reactive Protein Proteins 0.000 description 25
- 102100032752 C-reactive protein Human genes 0.000 description 25
- 241001678559 COVID-19 virus Species 0.000 description 25
- 239000012099 Alexa Fluor family Substances 0.000 description 22
- 241000700605 Viruses Species 0.000 description 18
- 210000002966 serum Anatomy 0.000 description 18
- 238000012360 testing method Methods 0.000 description 15
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 14
- 108010082126 Alanine transaminase Proteins 0.000 description 14
- 108091007413 Extracellular RNA Proteins 0.000 description 13
- 102100038104 Glycogen synthase kinase-3 beta Human genes 0.000 description 12
- 210000003296 saliva Anatomy 0.000 description 12
- 102100022463 Alpha-1-acid glycoprotein 1 Human genes 0.000 description 11
- 102000052508 Lipopolysaccharide-binding protein Human genes 0.000 description 11
- 108010053632 Lipopolysaccharide-binding protein Proteins 0.000 description 11
- 101710191428 Ubiquitin-like modifier-activating enzyme 7 Proteins 0.000 description 11
- 108010086596 glutathione peroxidase GPX1 Proteins 0.000 description 11
- 108010017992 platelet-derived growth factor C Proteins 0.000 description 11
- 238000000513 principal component analysis Methods 0.000 description 11
- 208000001528 Coronaviridae Infections Diseases 0.000 description 10
- 108091054442 EV proteins Proteins 0.000 description 10
- 150000001413 amino acids Chemical class 0.000 description 10
- 238000004891 communication Methods 0.000 description 10
- 238000002372 labelling Methods 0.000 description 10
- 108091028043 Nucleic acid sequence Proteins 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 210000003097 mucus Anatomy 0.000 description 9
- 238000000751 protein extraction Methods 0.000 description 9
- YSGQGNQWBLYHPE-CFUSNLFHSA-N (7r,8r,9s,10r,13s,14s,17s)-17-hydroxy-7,13-dimethyl-2,6,7,8,9,10,11,12,14,15,16,17-dodecahydro-1h-cyclopenta[a]phenanthren-3-one Chemical compound C1C[C@]2(C)[C@@H](O)CC[C@H]2[C@@H]2[C@H](C)CC3=CC(=O)CC[C@@H]3[C@H]21 YSGQGNQWBLYHPE-CFUSNLFHSA-N 0.000 description 8
- AQQSXKSWTNWXKR-UHFFFAOYSA-N 2-(2-phenylphenanthro[9,10-d]imidazol-3-yl)acetic acid Chemical compound C1(=CC=CC=C1)C1=NC2=C(N1CC(=O)O)C1=CC=CC=C1C=1C=CC=CC=12 AQQSXKSWTNWXKR-UHFFFAOYSA-N 0.000 description 8
- 102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 description 8
- 102100034594 Angiopoietin-1 Human genes 0.000 description 8
- 102100026349 Beta-1,4-galactosyltransferase 1 Human genes 0.000 description 8
- 102100036364 Cadherin-2 Human genes 0.000 description 8
- 102100022533 Calcium-binding protein 39 Human genes 0.000 description 8
- 102100024338 Collagen alpha-3(VI) chain Human genes 0.000 description 8
- 102100032636 Copine-1 Human genes 0.000 description 8
- 102100028907 Cullin-4A Human genes 0.000 description 8
- 102100021202 Desmocollin-1 Human genes 0.000 description 8
- 102100039950 Eukaryotic initiation factor 4A-I Human genes 0.000 description 8
- 102100037362 Fibronectin Human genes 0.000 description 8
- 102100027541 GTP-binding protein Rheb Human genes 0.000 description 8
- 102100030875 Gastricsin Human genes 0.000 description 8
- 102100035354 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Human genes 0.000 description 8
- 102100034154 Guanine nucleotide-binding protein G(i) subunit alpha-2 Human genes 0.000 description 8
- 102100036703 Guanine nucleotide-binding protein subunit alpha-13 Human genes 0.000 description 8
- 101000780453 Homo sapiens All-trans-retinol dehydrogenase [NAD(+)] ADH1B Proteins 0.000 description 8
- 101000678195 Homo sapiens Alpha-1-acid glycoprotein 1 Proteins 0.000 description 8
- 101000924552 Homo sapiens Angiopoietin-1 Proteins 0.000 description 8
- 101000784014 Homo sapiens Argininosuccinate synthase Proteins 0.000 description 8
- 101000766145 Homo sapiens Beta-1,4-galactosyltransferase 1 Proteins 0.000 description 8
- 101000714537 Homo sapiens Cadherin-2 Proteins 0.000 description 8
- 101000899411 Homo sapiens Calcium-binding protein 39 Proteins 0.000 description 8
- 101000855412 Homo sapiens Carbamoyl-phosphate synthase [ammonia], mitochondrial Proteins 0.000 description 8
- 101000910843 Homo sapiens Carboxypeptidase N catalytic chain Proteins 0.000 description 8
- 101000909506 Homo sapiens Collagen alpha-3(VI) chain Proteins 0.000 description 8
- 101000941754 Homo sapiens Copine-1 Proteins 0.000 description 8
- 101000916245 Homo sapiens Cullin-4A Proteins 0.000 description 8
- 101000761960 Homo sapiens Cytochrome P450 11B1, mitochondrial Proteins 0.000 description 8
- 101000968043 Homo sapiens Desmocollin-1 Proteins 0.000 description 8
- 101000880960 Homo sapiens Desmocollin-3 Proteins 0.000 description 8
- 101001027128 Homo sapiens Fibronectin Proteins 0.000 description 8
- 101001024316 Homo sapiens Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Proteins 0.000 description 8
- 101001070508 Homo sapiens Guanine nucleotide-binding protein G(i) subunit alpha-2 Proteins 0.000 description 8
- 101001072481 Homo sapiens Guanine nucleotide-binding protein subunit alpha-13 Proteins 0.000 description 8
- 101001078143 Homo sapiens Integrin alpha-IIb Proteins 0.000 description 8
- 101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 description 8
- 101001002695 Homo sapiens Integrin-linked protein kinase Proteins 0.000 description 8
- 101000599858 Homo sapiens Intercellular adhesion molecule 2 Proteins 0.000 description 8
- 101001046633 Homo sapiens Junctional adhesion molecule A Proteins 0.000 description 8
- 101001018097 Homo sapiens L-selectin Proteins 0.000 description 8
- 101001042351 Homo sapiens LIM and senescent cell antigen-like-containing domain protein 1 Proteins 0.000 description 8
- 101000619898 Homo sapiens Leukotriene A-4 hydrolase Proteins 0.000 description 8
- 101000958390 Homo sapiens Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA Proteins 0.000 description 8
- 101000983292 Homo sapiens N-fatty-acyl-amino acid synthase/hydrolase PM20D1 Proteins 0.000 description 8
- 101000582002 Homo sapiens Neuron navigator 2 Proteins 0.000 description 8
- 101000603202 Homo sapiens Nicotinamide N-methyltransferase Proteins 0.000 description 8
- 101000601047 Homo sapiens Nidogen-1 Proteins 0.000 description 8
- 101000851976 Homo sapiens Nucleoside diphosphate phosphatase ENTPD5 Proteins 0.000 description 8
- 101001067833 Homo sapiens Peptidyl-prolyl cis-trans isomerase A Proteins 0.000 description 8
- 101000605666 Homo sapiens Phospholipase A1 member A Proteins 0.000 description 8
- 101001053641 Homo sapiens Plasma serine protease inhibitor Proteins 0.000 description 8
- 101000947178 Homo sapiens Platelet basic protein Proteins 0.000 description 8
- 101001116302 Homo sapiens Platelet endothelial cell adhesion molecule Proteins 0.000 description 8
- 101001070786 Homo sapiens Platelet glycoprotein Ib beta chain Proteins 0.000 description 8
- 101001098868 Homo sapiens Proprotein convertase subtilisin/kexin type 9 Proteins 0.000 description 8
- 101000954831 Homo sapiens Protein MENT Proteins 0.000 description 8
- 101000979461 Homo sapiens Protein Niban 2 Proteins 0.000 description 8
- 101000964691 Homo sapiens Protein Z-dependent protease inhibitor Proteins 0.000 description 8
- 101000983130 Homo sapiens Protein kinase C and casein kinase substrate in neurons protein 2 Proteins 0.000 description 8
- 101001086862 Homo sapiens Pulmonary surfactant-associated protein B Proteins 0.000 description 8
- 101001112198 Homo sapiens Putative neutrophil cytosol factor 1B Proteins 0.000 description 8
- 101001077409 Homo sapiens Ras-related protein Rab-5B Proteins 0.000 description 8
- 101001130458 Homo sapiens Ras-related protein Ral-B Proteins 0.000 description 8
- 101001106082 Homo sapiens Receptor expression-enhancing protein 6 Proteins 0.000 description 8
- 101000686909 Homo sapiens Resistin Proteins 0.000 description 8
- 101000629622 Homo sapiens Serine-pyruvate aminotransferase Proteins 0.000 description 8
- 101000861263 Homo sapiens Steroid 21-hydroxylase Proteins 0.000 description 8
- 101000837443 Homo sapiens T-complex protein 1 subunit beta Proteins 0.000 description 8
- 101000763314 Homo sapiens Thrombomodulin Proteins 0.000 description 8
- 101000680271 Homo sapiens Transmembrane protein 59 Proteins 0.000 description 8
- 101001057508 Homo sapiens Ubiquitin-like protein ISG15 Proteins 0.000 description 8
- 102100025306 Integrin alpha-IIb Human genes 0.000 description 8
- 102100025304 Integrin beta-1 Human genes 0.000 description 8
- 102100020944 Integrin-linked protein kinase Human genes 0.000 description 8
- 102100037872 Intercellular adhesion molecule 2 Human genes 0.000 description 8
- 102100022304 Junctional adhesion molecule A Human genes 0.000 description 8
- 101710159002 L-lactate oxidase Proteins 0.000 description 8
- 102100033467 L-selectin Human genes 0.000 description 8
- 102100021754 LIM and senescent cell antigen-like-containing domain protein 1 Human genes 0.000 description 8
- 102100038245 Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA Human genes 0.000 description 8
- 102100026873 N-fatty-acyl-amino acid synthase/hydrolase PM20D1 Human genes 0.000 description 8
- 102100030465 Neuron navigator 2 Human genes 0.000 description 8
- 102100038951 Nicotinamide N-methyltransferase Human genes 0.000 description 8
- 102100037369 Nidogen-1 Human genes 0.000 description 8
- 102100036518 Nucleoside diphosphate phosphatase ENTPD5 Human genes 0.000 description 8
- 102100034539 Peptidyl-prolyl cis-trans isomerase A Human genes 0.000 description 8
- 102100038331 Phospholipase A1 member A Human genes 0.000 description 8
- 102100024078 Plasma serine protease inhibitor Human genes 0.000 description 8
- 102100036154 Platelet basic protein Human genes 0.000 description 8
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 description 8
- 102100034168 Platelet glycoprotein Ib beta chain Human genes 0.000 description 8
- 101710191567 Probable endopolygalacturonase C Proteins 0.000 description 8
- 102100038955 Proprotein convertase subtilisin/kexin type 9 Human genes 0.000 description 8
- 102000004885 Protease-activated receptor 4 Human genes 0.000 description 8
- 108090001010 Protease-activated receptor 4 Proteins 0.000 description 8
- 102100037056 Protein MENT Human genes 0.000 description 8
- 102100023075 Protein Niban 2 Human genes 0.000 description 8
- 102100040790 Protein Z-dependent protease inhibitor Human genes 0.000 description 8
- 102100026843 Protein kinase C and casein kinase substrate in neurons protein 2 Human genes 0.000 description 8
- 102100026858 Protein-lysine 6-oxidase Human genes 0.000 description 8
- 102100032617 Pulmonary surfactant-associated protein B Human genes 0.000 description 8
- 102100023615 Putative neutrophil cytosol factor 1B Human genes 0.000 description 8
- 101150020518 RHEB gene Proteins 0.000 description 8
- 102100025132 Ras-related protein Rab-5B Human genes 0.000 description 8
- 102100031425 Ras-related protein Ral-B Human genes 0.000 description 8
- 102100021075 Receptor expression-enhancing protein 6 Human genes 0.000 description 8
- 102100024735 Resistin Human genes 0.000 description 8
- 102100026842 Serine-pyruvate aminotransferase Human genes 0.000 description 8
- 102100028679 T-complex protein 1 subunit beta Human genes 0.000 description 8
- 102100026966 Thrombomodulin Human genes 0.000 description 8
- 102100022075 Transmembrane protein 59 Human genes 0.000 description 8
- 102100027266 Ubiquitin-like protein ISG15 Human genes 0.000 description 8
- 102100023895 Zyxin Human genes 0.000 description 8
- KMGARVOVYXNAOF-UHFFFAOYSA-N benzpiperylone Chemical compound C1CN(C)CCC1N1C(=O)C(CC=2C=CC=CC=2)=C(C=2C=CC=CC=2)N1 KMGARVOVYXNAOF-UHFFFAOYSA-N 0.000 description 8
- NJHLGKJQFKUSEA-UHFFFAOYSA-N n-[2-(4-hydroxyphenyl)ethyl]-n-methylnitrous amide Chemical compound O=NN(C)CCC1=CC=C(O)C=C1 NJHLGKJQFKUSEA-UHFFFAOYSA-N 0.000 description 8
- 108090000765 processed proteins & peptides Proteins 0.000 description 8
- 101000976393 Homo sapiens Zyxin Proteins 0.000 description 7
- 230000006866 deterioration Effects 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 7
- 238000011084 recovery Methods 0.000 description 7
- 239000006185 dispersion Substances 0.000 description 6
- 239000007850 fluorescent dye Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 6
- 238000007481 next generation sequencing Methods 0.000 description 6
- 101710094863 Acireductone dioxygenase Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 239000011324 bead Substances 0.000 description 5
- 230000015271 coagulation Effects 0.000 description 5
- 238000005345 coagulation Methods 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 5
- 108091070501 miRNA Proteins 0.000 description 5
- 239000002679 microRNA Substances 0.000 description 5
- 238000002493 microarray Methods 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 101710181757 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase Proteins 0.000 description 4
- 102100025222 CD63 antigen Human genes 0.000 description 4
- 101000934368 Homo sapiens CD63 antigen Proteins 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
- 238000002790 cross-validation Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 238000001215 fluorescent labelling Methods 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 101710186701 Alpha-1-acid glycoprotein 1 Proteins 0.000 description 3
- 108010009356 Cyclin-Dependent Kinase Inhibitor p15 Proteins 0.000 description 3
- 102000009512 Cyclin-Dependent Kinase Inhibitor p15 Human genes 0.000 description 3
- 101001026870 Homo sapiens Serine/threonine-protein kinase D1 Proteins 0.000 description 3
- 108010029485 Protein Isoforms Proteins 0.000 description 3
- 102000001708 Protein Isoforms Human genes 0.000 description 3
- 108700020978 Proto-Oncogene Proteins 0.000 description 3
- 102000052575 Proto-Oncogene Human genes 0.000 description 3
- 102100037310 Serine/threonine-protein kinase D1 Human genes 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 239000000090 biomarker Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000013068 control sample Substances 0.000 description 3
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 235000019253 formic acid Nutrition 0.000 description 3
- 238000001114 immunoprecipitation Methods 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 208000019423 liver disease Diseases 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 238000001543 one-way ANOVA Methods 0.000 description 3
- 230000003449 preventive effect Effects 0.000 description 3
- 238000000575 proteomic method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000005199 ultracentrifugation Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- VGIRNWJSIRVFRT-UHFFFAOYSA-N 2',7'-difluorofluorescein Chemical compound OC(=O)C1=CC=CC=C1C1=C2C=C(F)C(=O)C=C2OC2=CC(O)=C(F)C=C21 VGIRNWJSIRVFRT-UHFFFAOYSA-N 0.000 description 2
- GOLORTLGFDVFDW-UHFFFAOYSA-N 3-(1h-benzimidazol-2-yl)-7-(diethylamino)chromen-2-one Chemical compound C1=CC=C2NC(C3=CC4=CC=C(C=C4OC3=O)N(CC)CC)=NC2=C1 GOLORTLGFDVFDW-UHFFFAOYSA-N 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
- 102000015866 Adenylate kinase isoenzyme 1 Human genes 0.000 description 2
- 108050004092 Adenylate kinase isoenzyme 1 Proteins 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 108010048154 Angiopoietin-1 Proteins 0.000 description 2
- 102000009088 Angiopoietin-1 Human genes 0.000 description 2
- 102000015279 Basigin Human genes 0.000 description 2
- 108010064528 Basigin Proteins 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 2
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 101710137044 Fibrinogen alpha chain Proteins 0.000 description 2
- 101710170765 Fibrinogen beta chain Proteins 0.000 description 2
- 101710197507 Fibrinogen-like protein 1 Proteins 0.000 description 2
- 101710088172 HTH-type transcriptional regulator RipA Proteins 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- 108010052285 Membrane Proteins Proteins 0.000 description 2
- 108091092724 Noncoding DNA Proteins 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 108090000315 Protein Kinase C Proteins 0.000 description 2
- 102000003923 Protein Kinase C Human genes 0.000 description 2
- 239000012083 RIPA buffer Substances 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 101710114053 Ras-related protein Rab-13 Proteins 0.000 description 2
- 101710116844 Ras-related protein Rap-1b Proteins 0.000 description 2
- 102100028874 Sodium-dependent serotonin transporter Human genes 0.000 description 2
- 208000036142 Viral infection Diseases 0.000 description 2
- AZRNEVJSOSKAOC-VPHBQDTQSA-N [[(2r,3s,5r)-5-[5-[(e)-3-[6-[5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoylamino]prop-1-enyl]-2,4-dioxopyrimidin-1-yl]-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C(\C=C\CNC(=O)CCCCCNC(=O)CCCC[C@H]2[C@H]3NC(=O)N[C@H]3CS2)=C1 AZRNEVJSOSKAOC-VPHBQDTQSA-N 0.000 description 2
- 108090000449 aminopeptidase B Proteins 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 210000000234 capsid Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 208000029078 coronary artery disease Diseases 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 229940109239 creatinine Drugs 0.000 description 2
- 238000013135 deep learning Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 2
- 229960005156 digoxin Drugs 0.000 description 2
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 230000000984 immunochemical effect Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000001325 log-rank test Methods 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000000491 multivariate analysis Methods 0.000 description 2
- VYNDHICBIRRPFP-UHFFFAOYSA-N pacific blue Chemical compound FC1=C(O)C(F)=C2OC(=O)C(C(=O)O)=CC2=C1 VYNDHICBIRRPFP-UHFFFAOYSA-N 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000004393 prognosis Methods 0.000 description 2
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 238000010814 radioimmunoprecipitation assay Methods 0.000 description 2
- 238000000611 regression analysis Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 108010073531 rhoC GTP-Binding Protein Proteins 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 150000003431 steroids Chemical class 0.000 description 2
- WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 description 2
- JGVWCANSWKRBCS-UHFFFAOYSA-N tetramethylrhodamine thiocyanate Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=C(SC#N)C=C1C(O)=O JGVWCANSWKRBCS-UHFFFAOYSA-N 0.000 description 2
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 206010001052 Acute respiratory distress syndrome Diseases 0.000 description 1
- 101150011999 Adi1 gene Proteins 0.000 description 1
- 108010002020 Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Proteins 0.000 description 1
- 108091023037 Aptamer Proteins 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 102000004106 Claudin-3 Human genes 0.000 description 1
- 108090000599 Claudin-3 Proteins 0.000 description 1
- 235000005956 Cosmos caudatus Nutrition 0.000 description 1
- 101710195468 Cytochrome b-c1 complex subunit 2, mitochondrial Proteins 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 239000003154 D dimer Substances 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 238000000018 DNA microarray Methods 0.000 description 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
- 206010061818 Disease progression Diseases 0.000 description 1
- 208000032928 Dyslipidaemia Diseases 0.000 description 1
- 206010048554 Endothelial dysfunction Diseases 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 238000001134 F-test Methods 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 239000000232 Lipid Bilayer Substances 0.000 description 1
- 208000017170 Lipid metabolism disease Diseases 0.000 description 1
- 108020005198 Long Noncoding RNA Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 108091030146 MiRBase Proteins 0.000 description 1
- 208000009525 Myocarditis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000010220 Pearson correlation analysis Methods 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 108091007412 Piwi-interacting RNA Proteins 0.000 description 1
- 101710094033 Protein kinase C beta type Proteins 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 238000010802 RNA extraction kit Methods 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108090000340 Transaminases Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 206010047115 Vasculitis Diseases 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000000091 biomarker candidate Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012767 chemiluminescent enzyme immunoassay Methods 0.000 description 1
- 238000000546 chi-square test Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000005860 defense response to virus Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005750 disease progression Effects 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008694 endothelial dysfunction Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000002618 extracorporeal membrane oxygenation Methods 0.000 description 1
- 108010052295 fibrin fragment D Proteins 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007417 hierarchical cluster analysis Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000035992 intercellular communication Effects 0.000 description 1
- 238000005305 interferometry Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000011528 liquid biopsy Methods 0.000 description 1
- 230000005976 liver dysfunction Effects 0.000 description 1
- 108091028606 miR-1 stem-loop Proteins 0.000 description 1
- 238000003253 miRNA assay Methods 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 238000007857 nested PCR Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000000710 polymer precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 102000016914 ras Proteins Human genes 0.000 description 1
- 108010014186 ras Proteins Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 229940076279 serotonin Drugs 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000004055 small Interfering RNA Substances 0.000 description 1
- 206010041232 sneezing Diseases 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- HWCKGOZZJDHMNC-UHFFFAOYSA-M tetraethylammonium bromide Chemical compound [Br-].CC[N+](CC)(CC)CC HWCKGOZZJDHMNC-UHFFFAOYSA-M 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 102000014898 transaminase activity proteins Human genes 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000012762 unpaired Student’s t-test Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/118—Prognosis of disease development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/165—Coronaviridae, e.g. avian infectious bronchitis virus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/60—Complex ways of combining multiple protein biomarkers for diagnosis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020168107 | 2020-10-02 | ||
| JP2020-168107 | 2020-10-02 | ||
| PCT/JP2021/036525 WO2022071601A1 (ja) | 2020-10-02 | 2021-10-01 | 血中エクソソームのタンパク質マーカーを利用したｃｏｖｉｄ－１９重症化予測方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114556105A true CN114556105A (zh) | 2022-05-27 |
Family
ID=80951770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202180002820.2A Pending CN114556105A (zh) | 2020-10-02 | 2021-10-01 | 利用血中外泌体的蛋白质标志物的covid-19重症化预测方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20240263234A1 (https=) |
| EP (1) | EP4223887A4 (https=) |
| JP (1) | JPWO2022071601A1 (https=) |
| CN (1) | CN114556105A (https=) |
| WO (1) | WO2022071601A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119177277A (zh) * | 2024-10-08 | 2024-12-24 | 北京医院 | 与结肠癌细胞对吉西他滨耐药性相关的circ86591的应用 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023007809A1 (ja) * | 2021-07-29 | 2023-02-02 | 国立大学法人東北大学 | コロナウイルス感染症の重症化リスクの評価方法、およびコロナウイルス感染症の重症化リスク評価キット |
| CN115097147B (zh) * | 2022-08-23 | 2022-11-25 | 细胞生态海河实验室 | 预测奥密克戎复阳风险的生物标志物及代谢、蛋白、联合模型 |
| KR20250107848A (ko) | 2022-11-07 | 2025-07-14 | 아이에스엠 가부시키가이샤 | 코트머단백질 복합체 서브유닛 베타2에 대한 항체 |
| WO2024181522A1 (ja) * | 2023-02-28 | 2024-09-06 | 国立大学法人大阪大学 | Ｃｏｖｉｄ－１９を検査する方法 |
| CN117467598B (zh) * | 2023-12-28 | 2024-05-10 | 健颐生物科技发展(山东)有限公司 | 肝细胞源pcsk9外泌体的制备方法 |
| WO2025170060A1 (ja) * | 2024-02-08 | 2025-08-14 | 学校法人慈恵大学 | 急性肺障害治療薬 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103562725A (zh) * | 2011-04-15 | 2014-02-05 | 儿童医疗中心有限公司 | 川崎病的诊断标志物和治疗靶点 |
| CN111366728A (zh) * | 2020-03-27 | 2020-07-03 | 重庆探生科技有限公司 | 一种用于检测新型冠状病毒SARS-CoV-2的免疫层析试剂盒 |
-
2021
- 2021-10-01 EP EP21816304.6A patent/EP4223887A4/en active Pending
- 2021-10-01 US US17/618,412 patent/US20240263234A1/en active Pending
- 2021-10-01 JP JP2022554159A patent/JPWO2022071601A1/ja active Pending
- 2021-10-01 WO PCT/JP2021/036525 patent/WO2022071601A1/ja not_active Ceased
- 2021-10-01 CN CN202180002820.2A patent/CN114556105A/zh active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103562725A (zh) * | 2011-04-15 | 2014-02-05 | 儿童医疗中心有限公司 | 川崎病的诊断标志物和治疗靶点 |
| CN111366728A (zh) * | 2020-03-27 | 2020-07-03 | 重庆探生科技有限公司 | 一种用于检测新型冠状病毒SARS-CoV-2的免疫层析试剂盒 |
Non-Patent Citations (2)
| Title |
|---|
| CHANG ZHENG WANG: "Early risk factors of the exacerbation of coronavirus disease 2019 pneumonia", 《JOURNAL OF MEDICAL VIROLOGY》, vol. 92, no. 11, 21 August 2020 (2020-08-21), pages 2593, XP055891155, DOI: 10.1002/jmv.26071 * |
| CHRYSANTHI SKEVAKI: "Laboratory characteristics of patients infected with the novel SARS-CoV-2 virus", 《JOURNAL OF INFECTION》, 31 August 2020 (2020-08-31), pages 205 - 212 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119177277A (zh) * | 2024-10-08 | 2024-12-24 | 北京医院 | 与结肠癌细胞对吉西他滨耐药性相关的circ86591的应用 |
| CN119177277B (zh) * | 2024-10-08 | 2025-05-27 | 北京医院 | 与结肠癌细胞对吉西他滨耐药性相关的circ86591的应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4223887A8 (en) | 2023-11-22 |
| EP4223887A1 (en) | 2023-08-09 |
| WO2022071601A1 (ja) | 2022-04-07 |
| EP4223887A4 (en) | 2024-10-30 |
| JPWO2022071601A1 (https=) | 2022-04-07 |
| US20240263234A1 (en) | 2024-08-08 |
| TW202223104A (zh) | 2022-06-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114556105A (zh) | 利用血中外泌体的蛋白质标志物的covid-19重症化预测方法 | |
| TWI708058B (zh) | 阿茲海默症及其他神經退化性疾病之生物標記及診斷方法 | |
| CN107110867B (zh) | 用于肝癌诊断的生物标记物及其用途 | |
| CN114051536A (zh) | 利用血液中rna的covid-19重症化预测方法 | |
| US20090042201A1 (en) | Biomarkers for multiple sclerosis and methods of use thereof | |
| JPWO2022071601A5 (https=) | ||
| US9983215B2 (en) | Methods and compositions for diagnosis of ectopic pregnancy | |
| US20210116462A1 (en) | Methods and Compositions for the Diagnosis and Treatment of Kawasaki Disease | |
| EP2893039A1 (en) | Markers and methods for detecting posttraumatic stress disorder (ptsd) | |
| CN113286898B (zh) | 用于预测遗传性卵巢癌发生的生物标志物及其用途 | |
| CN113358881B (zh) | 用于nmosd预测或复发监测的生物标志物及其应用 | |
| CN113502326B (zh) | 基于生物标志物的肺动脉高压的诊断产品及其应用 | |
| US20140248637A1 (en) | Composition for diagnosis of lung cancer and diagnosis kit of lung cancer | |
| TWI921387B (zh) | 利用血中胞外體之蛋白質標記之covid-19重症化預測方法 | |
| KR101859812B1 (ko) | 간암 화학 색전술 치료 예후 예측을 위한 바이오마커 및 그 용도 | |
| JP2025502633A (ja) | 無痛形態から侵襲性前立腺ガンを検出するバイオマーカーおよびその治療 | |
| US11377690B2 (en) | Method and kit for making an in vitro prognosis of severity for a patient in septic shock | |
| CN114959003A (zh) | 急性心肌梗死标志物及其应用 | |
| CN111763736A (zh) | 用于诊断甲状腺乳头状癌淋巴结转移的液体活检试剂盒 | |
| US20260028679A1 (en) | Biomarker for diagnosis of gastric cancer | |
| KR101328391B1 (ko) | 유방암 환자의 화학요법제 저항성 예측을 위한 혈청 바이오마커 | |
| JPWO2011138955A1 (ja) | Ｓｉａα２−８Ｓｉａα２−３Ｇａｌβ−Ｒ糖鎖を持つムチン１の分析方法 | |
| EP2607494A1 (en) | Biomarkers for lung cancer risk assessment | |
| JP2025526256A (ja) | 試料品質評価の方法 | |
| CN114231613A (zh) | 干扰素刺激基因15在制备自身免疫性眼病早期诊断产品中的应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: Tokyo, Japan Applicant after: ISM, Inc. Applicant after: Gong Huguang Applicant after: Luogu Xiaoguang Address before: Tokyo, Japan Applicant before: International cosmic Medical Co.,Ltd. Applicant before: Gong Huguang Applicant before: Luogu Xiaoguang |