CN114521567B - Compound cockroach killing composition and preparation method and application thereof - Google Patents
Compound cockroach killing composition and preparation method and application thereof Download PDFInfo
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- CN114521567B CN114521567B CN202210174280.2A CN202210174280A CN114521567B CN 114521567 B CN114521567 B CN 114521567B CN 202210174280 A CN202210174280 A CN 202210174280A CN 114521567 B CN114521567 B CN 114521567B
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a compound cockroach killing composition, a preparation method and application thereof, and relates to the technical field of pesticides. The compound cockroach-killing composition comprises the following raw materials in parts by weight: 0.002-1 part of pesticide, 0.002-1 part of bactericide, 5-15 parts of protein powder, 8-15 parts of grease, 20-40 parts of starch matrix, 2-8 parts of sugar, 3-8 parts of attractant, 8-15 parts of excipient and 0.5-2 parts of preservative, wherein the pesticide is selected from the following components: one or more of imidacloprid, dinotefuran, indoxacarb and fipronil; the bactericide is selected from the group consisting of: one or more of Zhongshengmycin, zinc thiazole and copper hydroxide. The compound cockroach-killing composition can reduce the dosage of pesticides, reduce the drug resistance of cockroaches and improve the use safety.
Description
Technical Field
The invention relates to the technical field of pesticides, in particular to a compound cockroach killing composition, a preparation method and application thereof.
Background
The cockroaches mainly use warm and moist gaps as habitats, and the sewer and wall joints of buildings in daily life are very suitable for the survival of the cockroaches and are difficult to find by people. Cockroaches are a transmission medium of various pathogenic bacteria, and secretion and feces of the cockroaches contain various pathogenic bacteria, such as typhoid bacillus, salmonella, bacillus dysenteriae, bacillus proteus, pseudomonas aeruginosa and the like which are easy to cause allergic reaction of people, and the cockroaches can also transmit various intestinal diseases such as cholera, diarrhea, dysentery and the like. Cockroaches are therefore one of the most prevalent and difficult urban sanitary pests to control. At present, cockroaches are killed mainly by means of chemical control measures, the bait is an important dosage form for controlling German cockroaches, and the insecticidal components mainly comprise fipronil, imidacloprid, propoxur, indoxacarb and the like.
Fipronil is a phenylpyrazole broad-spectrum pesticide, mainly has stomach toxicity effect and has contact killing and internal absorption effects, and can block chloride ion channels of nerve cells by combining with gamma-aminobutyric acid (GABA) receptors on insect nerve central cell membranes to interfere with normal functions of insect nervous systems so as to cause death of insects.
Imidacloprid is an inhibitor of nicotinic acetylcholine receptor, has the effects of contact killing, stomach toxicity, systemic and the like, and mainly acts on the central nervous system of insects to paralyze and die the insects. Under the condition of higher concentration of imidacloprid components, the test insects can be knocked down quickly.
Indoxacarb is a novel oxadiazine pesticide developed by DuPont in the United states, and has the effects of contact killing and stomach poisoning by blocking sodium ion channels in insect nerve cells to act on target pests. As an insecticidal effective component for bait, indoxacarb has the advantages of no repellency to pests, mild poisoning symptoms, proper incubation period, capability of overcoming increasingly serious insecticide resistance and the like. In the field of sanitary pest control, the pesticide mainly controls houseflies or cockroaches in the form of bait.
The conventional pesticide pays attention to the direct toxicological effect of the active ingredient on pests, for example, cockroaches are killed by contact poisoning and stomach poisoning effect of cypermethrin in the patent application with publication number of CN108902172A, cockroaches are killed by inhibiting energy metabolism effect of fipronil in the patent application with publication number of CN106614770A, and the formulation of the patent application with publication number of CN104705328A contains components such as flufenoxuron and indoxacarb, and cockroaches are killed by blocking chitin synthesis, disordered nerve conduction and the like. However, the long-term continuous use of high doses of chemical pesticides of a single variety or single mode of action is likely to cause cockroaches to develop resistance to drugs, and the cockroach killing effect is poor, and presents a potential threat to the environment and human health.
Disclosure of Invention
In view of the above, it is necessary to provide a compound cockroach-killing composition, which comprises a pesticide and a bactericide, wherein the bactericide is used for inhibiting the growth of intestinal flora of cockroaches, thereby improving the insecticidal activity of the pesticide, reducing the dosage of the pesticide, reducing the drug resistance of cockroaches, and improving the use safety.
The compound cockroach killing composition comprises the following raw materials in parts by weight:
0.002-1 part of pesticide,
0.002-1 part of bactericide,
5-15 parts of protein powder,
8-15 parts of grease and the like,
20-40 parts of a starch substrate,
2-8 parts of saccharides,
3-8 parts of an attractant,
5-10 parts of excipient,
0.5-2 parts of preservative,
2-4 parts of magnesium stearate,
the insecticide is selected from: one or more of imidacloprid, dinotefuran, indoxacarb and fipronil; the bactericide is selected from the group consisting of: one or more of Zhongshengmycin, zinc thiazole and copper hydroxide.
The drug resistance mechanism of the cockroach pesticide is related to the diversity of intestinal microorganisms, the cockroach utilizes the intestinal microorganisms to generate resistance to the pesticide, on one hand, if the intestinal microorganisms of the cockroach are symbiotic with flora capable of degrading the pesticide, the flora can degrade the pesticide to reduce the toxicity to the cockroach, on the other hand, the host can also improve the drug resistance of the host flora to the pesticide through the immunological interaction of the host and the flora in the intestinal tracts. The research shows that after the intestinal flora of the German cockroach is removed, the drug resistance of the German cockroach to various pesticides is obviously reduced. At present, the main means for solving the drug resistance is to alternately apply drugs or compound pesticides with different action mechanisms. The invention combines the bactericide and the pesticide from the aspect of inhibiting the intestinal microorganisms of the cockroaches, and inhibits the growth and the propagation of the intestinal microorganisms of the cockroaches by using the bactericide, thereby reducing the action of the intestinal microorganisms on the drug resistance of the cockroaches, improving the insecticidal effect of the pesticide and providing a new method and thinking for preventing and controlling the cockroaches and delaying the drug resistance.
According to the invention, the bactericide which has obvious influence on the intestinal microorganisms of the German cockroaches is screened, and is compounded with the pesticides such as imidacloprid, dinotefuran, indoxacarb, fipronil and the like, so that the synergistic effect is obvious after the compounding, the bactericide can obviously inhibit the growth of intestinal flora of the cockroaches, reduce the drug resistance of the cockroaches, improve the insecticidal activity of the pesticides, reduce the dosage of the pesticides and improve the use safety of products. Compared with the cockroach killing composition without bactericide, the compound cockroach killing composition provided by the invention can effectively delay the occurrence and development of drug resistance of cockroaches and improve the cockroach killing effect of the product.
In one embodiment, the water-saving type solar cell panel further comprises 15-40 parts of water.
In one embodiment, the germicide is a mesogen and/or zinc thiazole. The bacteriostasis and sterilization effects of the Zhongshengmycin and the thiazole zinc are better than those of copper hydroxide.
In one embodiment, the protein powder is soy protein powder and/or whey protein powder.
In one embodiment, the oil is sesame oil and/or peanut oil.
In one embodiment, the starch substrate is selected from the group consisting of: one or more of wheat flour, corn flour and sweet potato powder.
In one embodiment, the saccharide is selected from: one or more of caramel, mel, and maltose.
In one embodiment, the attractant is fried sesame powder and/or fried peanut powder.
In one embodiment, the excipient is glycerol and/or lard.
In one embodiment, the preservative is methylparaben.
In one embodiment, the weight ratio of the insecticide to the bactericide is (1-10): (1-10).
In one embodiment, the weight ratio of the insecticide to the bactericide is (1-5): (1-5).
The invention also provides a preparation method of the compound cockroach killing composition, which comprises the following steps: weighing the raw materials, and uniformly mixing to obtain the compound cockroach killing composition.
In one embodiment, each raw material is weighed, liquid raw materials except water in the raw materials are mixed, and water is added and mixed uniformly to obtain liquid medicine A; mixing the rest materials except water, adding water, and mixing to obtain liquid medicine B; and uniformly mixing the liquid medicine A and the liquid medicine B to obtain the compound cockroach killing composition.
The invention also provides application of the compound cockroach-killing composition in preparing cockroach-killing bait, cockroach-killing granules, cockroach-killing tablets, cockroach-killing capsules or cockroach-killing spray.
Compared with the prior art, the invention has the following beneficial effects:
the compound cockroach-killing composition of the invention is prepared by compounding the bactericide and the pesticide, and the growth and the reproduction of intestinal microorganisms of cockroaches are inhibited by using the bactericide, so that the action of the intestinal microorganisms on the drug resistance of the cockroaches is reduced, the insecticidal effect of the pesticide is improved, and a novel method and thinking are provided for preventing and controlling the cockroaches and delaying the drug resistance. Compared with the cockroach killing composition without bactericide, the compound cockroach killing composition provided by the invention can effectively delay the occurrence and development of drug resistance of cockroaches, and improve the cockroach killing effect of the product.
Drawings
FIG. 1 is a graph showing the effect of various germicides on the total abundance of the intestinal flora of German cockroaches.
FIG. 2 shows the effect of different kinds of germicides on the colony growth of German cockroach intestinal bacteria.
FIG. 3 shows the effect of the different concentrations of the biotin on the intestinal bacteria of German cockroach.
Detailed Description
In order that the invention may be understood more fully, a more particular description of the invention will be rendered by reference to the preferred embodiments that are now set forth. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The raw materials used in the following examples and comparative examples are commercially available unless otherwise specified. "parts" refer to parts by weight.
Example 1
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.1 part of imidacloprid, 1 part of Zhongshengmycin, 25 parts of wheat flour, 12 parts of soybean protein powder, 10 parts of sesame oil, 3 parts of caramel, 6 parts of glycerin, 5.5 parts of lard, 3 parts of fried sesame powder, 3 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate.
The preparation method of the compound cockroach killing agent comprises the following steps:
(1) Weighing imidacloprid, sesame oil, glycerin and Zhongshengmycin, uniformly mixing, adding 10 parts of deionized water, and uniformly stirring at the speed of 650r/min to obtain a liquid medicine A.
(2) Weighing and mixing the rest raw materials, adding 20 parts of deionized water, stirring at 650r/min to dissolve the materials, slowly adding the liquid medicine A in a stirring state, heating to 70 ℃, and preserving heat for 8 hours to obtain the compound cockroach killing composition.
(3) The compound cockroach-killing composition is prepared into cockroach-killing products in the dosage forms of baits, granules, tablets, capsules, sprays and the like by adopting a conventional method in the field.
Example 2
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.2 part of imidacloprid, 0.8 part of Zhongshengmycin, 25 parts of wheat flour, 12 parts of soybean protein powder, 10 parts of sesame oil, 3 parts of caramel, 6 parts of glycerin, 5.5 parts of lard, 3 parts of fried sesame powder, 3 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 1.
Example 3
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.5 part of imidacloprid, 0.5 part of Zhongshengmycin, 25 parts of wheat flour, 12 parts of soybean protein powder, 10 parts of sesame oil, 3 parts of caramel, 6 parts of glycerin, 5.5 parts of lard, 3 parts of fried sesame powder, 3 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 1.
Example 4
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: the preparation method of the compound cockroach killing agent comprises 0.8 part of imidacloprid, 0.2 part of Zhongshengmycin, 25 parts of wheat flour, 12 parts of soybean protein powder, 10 parts of sesame oil, 3 parts of caramel, 6 parts of glycerin, 5.5 parts of lard, 3 parts of fried sesame powder, 3 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate, and the preparation method is basically the same as that of the embodiment 1.
Example 5
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 1 part of imidacloprid, 0.1 part of Zhongshengmycin, 25 parts of wheat flour, 12 parts of soybean protein powder, 10 parts of sesame oil, 3 parts of caramel, 6 parts of glycerin, 5.5 parts of lard, 3 parts of fried sesame powder, 3 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 1.
Example 6
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.002 part of fipronil, 0.02 part of Zhongshengmycin, 20 parts of corn meal, 15 parts of whey protein powder, 12 parts of peanut oil, 4 parts of maltose, 6 parts of glycerin, 6.5 parts of lard, 4 parts of fried sesame powder, 2 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate.
The preparation method of the compound cockroach killing agent comprises the following steps:
(1) Fipronil, peanut oil, glycerin and Zhongshengmycin are weighed, uniformly mixed, 10 parts of deionized water is added, and the mixture is uniformly stirred at the speed of 650r/min, so as to obtain liquid medicine A.
(2) Weighing and mixing the rest raw materials, adding 20 parts of deionized water, stirring at 650r/min to dissolve the materials, slowly adding the liquid medicine A in a stirring state, heating to 70 ℃, and preserving heat for 8 hours to obtain the compound cockroach killing composition.
(3) The compound cockroach-killing composition is prepared into cockroach-killing products in the dosage forms of baits, granules, tablets, capsules, sprays and the like by adopting a conventional method in the field.
Example 7
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.004 part of fipronil, 0.02 part of Zhongshengmycin, 20 parts of corn meal, 15 parts of whey protein powder, 12 parts of peanut oil, 4 parts of maltose, 6 parts of glycerin, 6.5 parts of lard, 4 parts of fried sesame powder, 2 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 6.
Example 8
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.01 part of fipronil, 0.01 part of Zhongshengmycin, 20 parts of corn meal, 15 parts of whey protein powder, 12 parts of peanut oil, 4 parts of maltose, 6 parts of glycerin, 6.5 parts of lard, 4 parts of fried sesame powder, 2 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 6.
Example 9
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.02 part of fipronil, 0.004 part of Zhongshengmycin, 20 parts of corn meal, 15 parts of whey protein powder, 12 parts of peanut oil, 4 parts of maltose, 6 parts of glycerin, 6.5 parts of lard, 4 parts of fried sesame powder, 2 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 6.
Example 10
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.02 part of fipronil, 0.002 part of Zhongshengmycin, 20 parts of corn meal, 15 parts of whey protein powder, 12 parts of peanut oil, 4 parts of maltose, 6 parts of glycerin, 6.5 parts of lard, 4 parts of fried sesame powder, 2 parts of fried peanut powder and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 6.
Example 11
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.02 part of indoxacarb, 0.2 part of Zhongshengmycin, 10 parts of corn meal, 15 parts of sweet potato meal, 8 parts of peanut oil, 7 parts of sesame oil, 6 parts of whey protein powder, 3 parts of fried sesame powder, 3 parts of fried peanut powder, 5 parts of honey, 6 parts of glycerol, 6.5 parts of lard and 1 part of methyl p-hydroxybenzoate.
The preparation method of the compound cockroach killing agent comprises the following steps:
(1) Weighing indoxacarb, glycerol, sesame oil, peanut oil, zhongshengmycin and honey, uniformly mixing, adding 10 parts of deionized water, and uniformly stirring at the speed of 750r/min to obtain liquid medicine A.
(2) Weighing and mixing the rest raw materials, adding 20 parts of deionized water, stirring at 650r/min to dissolve the materials, slowly adding the liquid medicine A in a stirring state, heating to 70 ℃, and preserving heat for 8 hours to obtain the compound cockroach killing composition.
(3) The compound cockroach-killing composition is prepared into cockroach-killing products in the dosage forms of baits, granules, tablets, capsules, sprays and the like by adopting a conventional method in the field.
Example 12
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.04 part of indoxacarb, 0.2 part of Zhongshengmycin, 10 parts of corn flour, 15 parts of sweet potato powder, 8 parts of peanut oil, 7 parts of sesame oil, 6 parts of whey protein powder, 3 parts of fried sesame powder, 3 parts of fried peanut powder, 5 parts of honey, 6 parts of glycerol, 6.5 parts of lard and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 11.
Example 13
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.1 part of indoxacarb, 0.1 part of Zhongshengmycin, 10 parts of corn meal, 15 parts of sweet potato meal, 8 parts of peanut oil, 7 parts of sesame oil, 6 parts of whey protein powder, 3 parts of fried sesame powder, 3 parts of fried peanut powder, 5 parts of honey, 6 parts of glycerol, 6.5 parts of lard and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 11.
Example 14
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.2 part of indoxacarb, 0.04 part of Zhongshengmycin, 10 parts of corn flour, 15 parts of sweet potato powder, 8 parts of peanut oil, 7 parts of sesame oil, 6 parts of whey protein powder, 3 parts of fried sesame powder, 3 parts of fried peanut powder, 5 parts of honey, 6 parts of glycerol, 6.5 parts of lard and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 11.
Example 15
The compound cockroach killing medicament is prepared from the following raw materials in parts by weight: 0.2 part of indoxacarb, 0.02 part of Zhongshengmycin, 10 parts of corn meal, 15 parts of sweet potato meal, 8 parts of peanut oil, 7 parts of sesame oil, 6 parts of whey protein powder, 3 parts of fried sesame powder, 3 parts of fried peanut powder, 5 parts of honey, 6 parts of glycerol, 6.5 parts of lard and 1 part of methyl p-hydroxybenzoate. The preparation method of the compound cockroach killing agent is basically the same as that of the embodiment 11.
Experimental example 1
Zhongshengmycin, zinc thiazole and Cu (OH) 2 The sterilization effect test of (2).
The following formulations were formulated and tested for bacteriostatic effects.
Table 1 antimicrobial formulation
Soaking 0.5. 0.5 g artificial feed in the liquid medicine for 30 seconds, and then airing at room temperature for 6 hours. Taking 10 adults of the starved German cockroach, feeding the adults with the treated feed for 48 hours, and dissecting the German cockroach under the aseptic condition. 3 males and 3 females are respectively taken for dissection in each treatment, 200 mu l of sterile water is added into the obtained intestinal tract for homogenization, and then 800 mu l of sterile water is added for mixing. Gradient dilution of the resulting homogenate to 10 -2 And 10 -3 100 μl of each was uniformly spread on LB without anti-solid, incubated at 30deg.C for 24-48 hours, and finally the colony count on the plate was counted. In addition, 3 males and 3 females are respectively taken for dissection in each treatment, and DNA extraction is carried out on the obtained intestinal tracts. After extraction, uniformly diluting the DNA to 5 ng/mu l, and according to total bacterial primers, selecting German cockroach actin genes as internal references, and quantitatively detecting intestinal microorganism change by fluorescence.
The results are shown in fig. 1 and 2. The results show that the three components of copper hydroxide, zinc thiazole and Zhongshengmycin all significantly change the abundance of the cockroach intestinal flora, wherein the abundance of the cockroach intestinal flora treated by the copper hydroxide reaches 1.5, which is significantly higher than that of the control treatment. The abundance of cockroach intestinal flora is extremely reduced by about 0.1 under the treatment of zinc thiazole and Zhongshengmycin, and the abundance is extremely lower than that of the control treatment. The results prove that the three bactericides have strong interference capability on the abundance of intestinal flora of cockroaches, in particular to Zhongshengmycin and zinc thiazole.
Experimental example 2
Test of the inhibition of the intestinal microorganisms of German cockroach by the Zhongshengmycin.
100 German cockroaches (50 in each of the male and female) which were starved for 2 days were placed in a square box, the cockroach house was simulated, and the bait without the addition of the zhongshengmycin was used as a CK control treatment. Bait with three concentrations of 500 ppm, 1000ppm and 2000ppm of the added amount of the Zhongshengmycin is used as a test formula to be fed to the cockroaches for 7 days, and the total flora of the intestinal microorganisms of the cockroaches fed to the cockroaches for 1 day, 4 days and 7 days is measured and analyzed respectively, and the test is repeated for 3 times.
The results are shown in FIG. 3. The result shows that the addition of the mesogenic fungi in the formula effectively inhibits the total flora of the intestinal tracts of the cockroaches, and particularly, the inhibition rate is more remarkable along with the delay of feeding time. The results of intestinal flora count for cockroaches fed for 7 days showed that the tested formulation was significantly lower than the control treatment, and the higher the concentration used, the lower the total intestinal flora count (fig. 3 a). As a result of the above treatment of the form of the intestinal culturable bacteria (FIG. 3 c), the number of intestinal culturable colonies of cockroaches in the control group without adding the metazoan increased with the increase of the growth time of cockroaches. The addition of the mesogenic agent can inhibit intestinal microorganisms for a long time, so that colony numbers cannot be seen in vitro. In particular at concentrations of 1000ppm and 2000ppm, no culturable flora was found in the cockroach gut after 7 days of feeding. The inhibiting effect of the zhongshengmycin on the sustaining effect of intestinal microorganisms can be seen.
Experimental example 3
And compounding the biotin and the imidacloprid at different concentrations for toxicological tests of the German cockroach.
1. Test subject: german cockroach, indoor strain.
2. Test reagent: weighing imidacloprid raw material, dissolving with proper amount of acetone, and preparing mother solution with required concentration by 0.1% triton X-100; weighing the crude Zhongshengmycin, and preparing mother liquor with required concentration by using a proper amount of distilled water; mixing the mother solutions according to a certain proportion to prepare a mixed mother solution, wherein the mixed mother solution is prepared into a series of baits.
3. The test method comprises the following steps: reference to the indoor efficacy test of a hygienic insecticide for pesticide registration and evaluation part 7: the bait is prepared by starving German cockroaches for 2 days in a square box, putting 30 German cockroaches in the square box, adding 3g of the bait, testing the room temperature to 25 ℃ and the relative humidity to 70%, repeating the steps, taking the bait without medicine as a blank control, investigating the death and living insect numbers 24 hours, 48 hours and 72 hours after feeding, and calculating LC by using SPSS software 50 LC (liquid Crystal) and LC 95 . The results are shown in the following table.
Virulence of the two in Table 2 at different concentrations
4. Test results: the compound result of the Zhongshengmycin and the imidacloprid shows that the Zhongshengmycin has remarkable synergism on the insecticidal activity of the imidacloprid, and the synergism is more obvious along with the increase of the concentration of the Zhongshengmycin.
Experimental example 4
The test of the compound cockroach killing agent of different embodiments is a test of killing effect on German cockroaches.
1. Testing insects: 50 German cockroaches are respectively used for male and female, and 100 cockroaches are used for total.
2. Test treatment: the control group n-1 is a formulation in which the antibiotic in the bactericide is not added on the basis of the example n (n=1 to 15, n is an integer), and the control group n-2 is a formulation in which the insecticide is not added on the basis of the example n (n=1 to 15). Other ingredients and preparation methods of control groups n-1 and n-2 were identical to example n.
3. The test method comprises the following steps: 100 German cockroaches which are starved for 2 days are put into a square box, a cockroach house is simulated, 5 g of the compound cockroach killing agent of the embodiment 1 is taken, and the effect of the control group 1 is compared with that of the control group 2. Respectively placing at two ends of diagonal line, and placing enough food at two points of diagonal line for cockroach to eat. The number of dead insects was counted and observed daily after administration until all the insects tested died, 3 replicates and an average was taken.
4. The results are shown in the following tables, respectively.
TABLE 3 test results for example 1 and control 1-1, control 1-2
As can be seen from the table above, the cockroach killing effect of the control group 1-1 is slow, and the maximum cockroach killing effect is only 50% within 4 days. Control 2 had a weaker killing effect on cockroaches, with 20% of cockroaches dying on the fourth day. In contrast, example 1 showed the best effect, and the death rate of cockroaches was 60% on the first day and 100% on the fourth day.
TABLE 4 test results for example 2 and control group 2-1, control group 2-2
From the above table, the cockroach mortality rate reached 50% at day 1 of administration of the control group 2-1, but the effect was not significantly improved over time, and the cockroach mortality rate was only improved to 56.67% after four days of administration. Control group 2-2 had a weaker killing effect on cockroaches. The first day of the administration of example 2 had 56.66% of the killing effect, which is equivalent to the effect of the control group 2-1 on the fourth day, and the death rate of cockroaches was continuously increased over time, and the death rate after four days of administration reached 96.66%, which was very different from that of the control group.
TABLE 5 test results for example 3 and control 3-1, control 3-2
Compared with the imidacloprid of the embodiment 1 and the embodiment 2, the imidacloprid of the embodiment 3 has higher content, and the imidacloprid is obviously improved on the cockroach killing effect by improving the imidacloprid content, but the compounding effect of the imidacloprid and the Zhongshengmycin is still optimal. Control 3-1 achieved 73.33% mortality of cockroaches on the first day of administration and 93.33% on the fourth day. However, the cockroach mortality rate reached 83.33% on the first day and 100% all died on the fourth day in example 3. The effect of example 3 was still significantly higher than the control.
TABLE 6 test results for example 4 and control 4-1, control 4-2
The control group 4-1 reached a maximum of 96.67% after 3 days of administration, but the overall effect was not as good as in example 4, and the cockroaches all died after three days of administration in example 4. Although the content of the antibiotic in the embodiment is reduced compared with the embodiments 1-3, the synergistic effect of the antibiotic compound with the imidacloprid with high content is still very remarkable.
TABLE 7 test results for example 5 and control 5-1, control 5-2
This example further demonstrates that increasing the mortality of cockroaches by simply increasing the imidacloprid content is limited. Even though the imidacloprid content reaches 1%, if the mesogen is not compounded, the cockroach still has 3.33 percent of survival rate after the control group is administrated for four days 5-1. In example 5, however, 1% imidacloprid was compounded with only 0.1% of the mesogen, and cockroaches all died three days after administration.
TABLE 8 test results for example 6 and control 6-1, control 6-2
The cockroach killing effect of the control group 6-1 is slow, and the maximum cockroach killing effect is only 76.67% within 4 days. The control group 6-2 had a weak killing effect on cockroaches. The effect of the compound example 6 of the Zhongshengmycin and the fipronil is best, the death rate of cockroaches is 63.33% in the first day of administration, and the death rate reaches 93.33% in the fourth day.
TABLE 9 test results for example 7 and control 7-1, control 7-2
The cockroach killing effect of the control group 7-1 is slow, and the maximum cockroach killing effect is only 83.33% within 4 days. The control group 7-2 had a weak killing effect on cockroaches. Whereas example 7, in which the Zhongshengmycin and fipronil were compounded, had the best effect, the death rate of cockroaches was 76.67% in the first day and reached 100% in the fourth day.
TABLE 10 test results for example 8 and control 8-1, control 8-2
Control 8-1, although reaching 73.33% on the first day, was still less effective than example 8. Whereas example 8, in which the Zhongshengmycin and fipronil were compounded, had the best effect, the death rate of cockroaches was 86.67% in the first day, and reached 100% in the fourth day.
TABLE 11 test results for example 9 and control 9-1, control 9-2
The cockroach killing effect can be effectively improved by increasing the dosage of fipronil, the cockroach killing rate reaches 90% in one day of treatment of the control group 9-1, and reaches 100% in the third day. Whereas example 9 was administered with a first day cockroach mortality rate of up to 96.67% and a third day of 100%.
TABLE 12 test results for example 10 and control 10-1, control 10-2
The group of experiments further proves that the effect of improving the death rate of the cockroaches by simply increasing the fipronil content is limited. Even though fipronil content reached 0.005%, the cockroach still had a survival rate of 6.77% after two days of administration of the control group 10-1 if the mesogen was not compounded. However, fipronil at 0.005% was only compounded with the mesogen at 0.0005% (example 10), and cockroaches all died after two days of administration. Proves the importance of the antibiotic in the pesticide compounding.
TABLE 13 test results for example 11 and control 11-1, control 11-2
The cockroach killing effect of the control group 11-1 is slow, and the maximum of the cockroach killing effect is only 63.33% within four days. Control 11-2 had a smaller killing effect on cockroaches, with 20% of cockroaches dying on the fourth day. The best effect of the compound example 11 of the Zhongshengmycin and the indoxacarb is achieved, the death rate of cockroaches is 60% in the first day of administration, and the death rate reaches 100% in the fourth day.
TABLE 14 test results for example 12 and control 12-1, control 12-2
The control group 12-1 has slow cockroach killing effect, the first-day insecticidal rate of administration is only 40%, and the highest insecticidal rate in four days is only 76.77%. The compound example 12 of the Zhongshengmycin and the indoxacarb has the best effect, and the death rate of cockroaches is 66.67% in the first day and reaches 96.66% in the fourth day.
TABLE 15 test results for example 13 and control 13-1, control 13-2
The cockroach killing effect of the control group 13-1 is slow, and the maximum of the cockroach killing effect is only 96.67% within four days. The best effect of the compound example 13 of the Zhongshengmycin and the indoxacarb is achieved, the death rate of cockroaches is 86.67% in the first day of administration, and reaches 100% in the fourth day.
TABLE 16 test results for example 14 and control 14-1, control 14-2
The cockroach killing effect of the control group 14-1 is slow, and the maximum of the cockroach killing effect is only 96.67% within four days. The best effect of the compound example 14 of the Zhongshengmycin and the indoxacarb is achieved, the death rate of cockroaches is 83.33% in the first day of administration, and reaches 100% in the third day.
TABLE 17 test results for example 15 and control group 15-1, control group 15-2
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The group of experiments further proves that the effect of improving the death rate of cockroaches by simply increasing the content of indoxacarb is limited. The Zhongshengmycin plays an important role in killing cockroaches, when the dosage of Zhongshengmycin is reduced, the cockroach killing effect is weakened, the insecticidal rate reaches 83.33% after the control group 15-1 is administrated for one day, and the insecticidal rate reaches 96.67% after the control group is administrated for the fourth day. The best effect of the compound example 15 of the Zhongshengmycin and the indoxacarb is achieved, the death rate of cockroaches is 90% in the first day of administration, and the death rate reaches 100% in the fourth day. Therefore, after the indoxacarb and the Zhongshengmycin are compounded, the use effect is better than that of the independent indoxacarb under the same dosage.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (8)
1. The compound cockroach killing composition is characterized by comprising the following raw materials in parts by weight:
the insecticide is selected from: one or more of imidacloprid, dinotefuran, indoxacarb and fipronil;
the bactericide is Zhongshengmycin and/or zinc thiazole;
the weight ratio of the pesticide to the bactericide is (1-10): (1-10).
2. The compound cockroach killing composition according to claim 1, further comprising 15-50 parts of water.
3. The compound cockroach killing composition according to claim 1, wherein the protein powder is soybean protein powder and/or whey protein powder, the grease is sesame oil and/or sesame oil, and the starch substrate is selected from the group consisting of: one or more of wheat flour, corn flour and sweet potato powder, wherein the sugar is selected from the group consisting of: one or more of caramel, mel, and maltose.
4. The compound cockroach killing composition according to claim 1, wherein the attractant is fried sesame powder and/or fried peanut powder, and the excipient is glycerin and/or lard.
5. The compounded cockroach-killing composition of claim 1, wherein the preservative is methylparaben.
6. The compound cockroach-killing composition according to claim 1, wherein the weight ratio of the pesticide to the bactericide is (1-5): (1-5).
7. A method for preparing the compound cockroach killing composition according to any one of claims 1 to 6, which is characterized by comprising the following steps: weighing the raw materials, and uniformly mixing to obtain the compound cockroach killing composition.
8. Use of the compounded cockroach-killing composition according to any one of claims 1 to 6 in preparing cockroach-killing bait, cockroach-killing granule, cockroach-killing tablet, cockroach-killing capsule or cockroach-killing spray.
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