CN114521377B - Method for calculating water and fertilizer requirement rule of masson pine seeding based on water and fertilizer regulation - Google Patents
Method for calculating water and fertilizer requirement rule of masson pine seeding based on water and fertilizer regulation Download PDFInfo
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Abstract
The invention discloses a method for calculating a water and fertilizer requirement rule of masson pine seeding based on water and fertilizer regulation, which is to develop a precise water and fertilizer coupling test of the masson pine seeding in different growth and development stages on the premise of implementing positioning, quantification and timely water and fertilizer supplementation on a masson pine root region in space and time according to the distribution characteristics of a masson pine seeding root system along with a growth process in a matrix, count irrigation quantity and fertilization quantity, moisture evaporation quantity, moisture loss quantity, water storage quantity in the matrix and nutrient residue condition in recovery liquid and the matrix in different growth and development stages of different water and fertilizer treatment, the method can realize the dynamic and accurate control of the water and fertilizer supply amount in time and space, ensure the consistency of water and nutrients in the root zone of the nursery stock, finely master the source and destination of the water and fertilizer, and accurately acquire the water and fertilizer absorption rule and absorption amount of the nursery stock.
Description
Technical Field
The invention belongs to the technical field of masson pine planting, and particularly relates to a method for calculating a water and fertilizer requirement rule of masson pine seeding seedlings based on water and fertilizer regulation.
Background
The Pinus massoniana (Pinus massoniana) is an extremely important pioneer tree species for both wood and lipid in China, is one of a few tree species cultivated by a large-diameter wood at present, has the advantages of drought resistance, barren resistance, strong adaptability, high yield and the like, is a preferred tree species for afforestation of barren mountains, adjustment of forestry structures and construction of industrial raw materials, and has great economic and ecological benefits. Although China has a large planting area of the masson pine, the afforestation quality is low, one reason is that the physiological quality and the activity performance of the masson pine seeding are low, the forestry production only pays attention to the morphological index of the seeding, and the quality and the activity level of the seeding are ignored. The higher the seeding rate, the better the seeding rate, and the higher the seeding rate may have some advantages in genetics, but the better the size of the seeding rate in forestation. Therefore, the cultivation of high-quality and strong seeding seedlings is the key for improving the survival rate of afforestation.
To improve the quality of the sowed seedlings, the water and fertilizer supply and control in the seedling raising process are of great importance. The essential problem that will solve at first is exactly to master the liquid manure demand rule of seeding seedling in different stages of development, just can accomplish to supply as required, carries out the precision and grows seedlings in order to carry out the accurate supply of liquid manure at the in-process of growing seedlings. The method comprises the steps of accurately mastering the water and fertilizer requirement rules and the required quantity of the water and fertilizer requirement rules in each growth period of a seeding seedling, accurately regulating and controlling the water and nutrient content in a seeding seedling root matrix, regularly counting the irrigation quantity, the fertilization quantity, the water evaporation quantity, the water and nutrient shunt loss quantity and the water storage quantity and nutrient residual condition in the matrix under the premise of ensuring that the nutrients and the water in the seeding seedling root matrix under different water and fertilizer conditions are kept within a target control range in real time, calculating the water consumption and the absorbed mineral content in different growth periods of the pinus massoniana in different growth development stages and the same growth development stage, and accurately setting up the water and fertilizer requirement rules in different growth periods of the pinus massoniana in different growth development stages and the same growth development stage.
At present, the water and fertilizer management in the seedling raising process of the pinus massoniana relies on manual operation, the human subjectivity is strong, only the water and fertilizer application is managed, whether the seedlings need to be watered or not is not managed, and particularly, the excessive watering not only causes nutrient loss and damages the growth environment of the sowed seedlings, but also causes water resource pollution; excessive and insufficient water in fertilization can create double stress of the growth environment of the nursery stock, unbalanced water and nutrient absorption and low utilization rate of water and fertilizer; in addition, unbalanced fertilization can cause excessive or insufficient certain elements, and the symptoms of excessive poisoning or nutrient deficiency of certain elements appear, so that vegetative growth is vigorous or growth is hindered and metabolic disturbance is caused, and the normal growth of seedlings is influenced.
Therefore, research is carried out on the water and fertilizer requirement rule of the pinus massoniana seedlings and the water and fertilizer requirement amount by scientific research workers by utilizing a traditional method from the aspect of single factor effect, and the water and fertilizer requirement rule of the pinus massoniana seedlings is researched by respectively setting different water and nutrient gradients from the aspect of single factor and adopting a traditional weighing method and a water culture method; the optimal nitrogen-phosphorus-potassium combination beneficial to the growth of the pinus massoniana in the seedling stage and the absorption and accumulation of nutrients is preliminarily explored from the aspects of the growth and development of seedlings and the accumulation of nutrients in vivo. However, the research is to avoid water or nutrient and research the nutrient or water consumption rule of the nursery stock from a single factor level, and particularly, the research on the nutrient absorption rule of the nursery stock by water culture is unreasonable and scientific under the condition that the nursery stock forms a waterlogging environment and the growth and development and nutrient absorption of the nursery stock are influenced. Whether the nursery stock is healthy or not and the water and nutrient absorption are the result of water-fertilizer interaction, the reasonable water-fertilizer combination is beneficial to the nursery stock to absorb the water and fertilizer, and the over-high or over-low water and fertilizer is not beneficial to the nursery stock to grow and absorb and utilize the water and fertilizer.
At present, due to the lack of scientific research and experiment devices, accurate masson pine water and fertilizer demand rules and water and fertilizer demand quantity thereof are difficult to obtain, and become a bottleneck of reasonable and scientific water and fertilizer management seedling culture of masson pine. Therefore, the key problem to be solved by the invention is how to obtain the more accurate water and fertilizer requirement rule and the water and fertilizer requirement amount of the masson pine in different growth and development stages.
Disclosure of Invention
The invention provides a method for calculating a water and fertilizer requirement rule of masson pine seeding based on accurate water and fertilizer regulation, which comprises the following steps:
a, calculating the water consumption and the nutrient absorption required by increasing 1g of dry matter per accumulation of single seedlings in different growth and development stages of pinus massoniana sown seedlings under different water and fertilizer treatment through a water and fertilizer coupling test;
b, optimizing and finding out the optimal water and fertilizer combination of the nursery stock in different growth stages by adopting a multi-objective comprehensive decision model;
and C, respectively regulating and controlling the optimally determined optimal water and fertilizer combinations of the pinus massoniana seeding seedlings in different development stages to different growth and development stages of the seedlings, and calculating the water and fertilizer requirements and the water and fertilizer requirements of the seedlings in different growth and development stages and different growth periods in the same growth and development stage under the optimal water and fertilizer conditions.
Further, the water and fertilizer coupling test is an orthogonal test which is respectively provided with four different irrigation levels of full irrigation, mild deficit irrigation, moderate deficit irrigation and severe deficit irrigation and four different fertilization levels of super high fertilizer, high fertilizer, medium fertilizer and low fertilizer; the fertilizer is nitrogen, phosphorus and potassium fertilizer; the masson pine seeding seedling is divided into different growth and development stages: seedling emergence stage, initial growth stage, fast growth stage, late growth stage and dormancy stage;
further, according to the annual growth rule of the root system of the pinus massoniana seeding seedlings, water and fertilizer management in the water and fertilizer coupling test process adopts a precise water and fertilizer regulation and control system to carry out space-time dynamic precise control on water and fertilizer supply quantity on water and nutrients in the root area of the seedlings, so that the water and nutrient content in the matrix of the root area of the seedlings in different growth and development stages is guaranteed to be kept within a target threshold range in real time;
further, the water storage capacity (WMS) in the seedling substrate treated by different water fertilizers is calculated and recorded on the same day of sowing T-0 ) And residual amount of nutrients (C) T-m-0 ) And T: is a certain period of time; m: nitrogen, phosphorus and potassium;
further, after sowing, the amount of water irrigated during the time period for each treatment (I) was periodically counted Ti ) Nutrient application rate (E) Ti-m ) Water loss (Z) Ti ) And nutrient loss (R) Ti-m ) Water storage capacity in the matrix (WMS) Ti ) And residual amount of nutrients (C) Ti-m ) And i: is a certain period of time;
furthermore, the water consumption (W) required by increasing 1g dry matter for each single seedling in different growth and development stages of each processed masson pine seedling T-n ) And the amount of nutrients (N) to be absorbed T-m-n ) The following calculation formula is adopted for calculation:
in the formulae (1) and (2), N T-m-n And W T-n Respectively increasing the amount of absorbed nutrients and the amount of consumed water for every 1g of dry matter accumulated in each seedling in each growth and development stage of different water and fertilizer treatments; NU T-m-ni And ET T-ni Respectively the nutrient consumption and the water consumption of all the seedlings in a certain time period in each growth and development stage, W T-n The units L, N T-m-n The unit g; a: the total number of the seedlings in each growth and development stage; n: at different growth and development stages; b is T-m-n-1 And B T-m-n : respectively the total dry weight of the whole plants of a certain number of nursery stocks in unit g at the end of the last growth and development stage and the end of the growth and development stage;
water consumption (ET) of all the seedlings in said certain period i ) The method adopts a water balance method for calculation, and the formula is as follows:
ET i =I i -D i -Z i +(MWS i-1 -MWS i ) (3)
in formula (3), ET i : the unit L is the water consumption of all the seedlings in a certain time period; i is i : is the irrigation volume in a certain time period, in units of L; d i : the unit L is the water loss in a certain time period; z i : the water evaporation amount of the seedling culture land in a certain time period; the unit L; MWS i : the water storage capacity in the seedling substrate at the end of a certain time period is unit L; MWS i-1 : the unit L is the water storage capacity in the seedling substrate at the beginning of a certain time period; because the greenhouse and the seedling raising device are adopted as planting environments, the influences of precipitation, groundwater and surface runoff are not considered;
the water evaporation amount (Z) of the seedling raising land in a certain period i ) Measured by an evaporator which adopts a bottom beltThe method comprises the following steps of respectively burying a cylinder with a drain hole between seedling row spacing, arranging a substrate which is the same as a seedling growing seedling in an evaporator, enabling the substrate plane in the evaporator to be flush with the seedling growing substrate plane, weighing by adopting an electronic balance with the precision of 0.01g every day, recording the weight (M), and calculating the evaporation capacity of a seedling growing ground according to a formula (4):
Z i =S·Δt×Evr i (4)
the Evr i The evaporation rate of a seedling raising land per unit area is the unit g cm -2 ·h -1 The calculation formula is as follows:
in the formula (4), (5), M 1 And M 2 Is t 1 And t 2 Total weight of time evaporator, in g; s: is the area of the inner tube of the evaporator in cm 2 (ii) a Δ t: the time interval of two adjacent weighing times is unit h; s: is the area of a seedling raising land in cm 2 ;
Nutrient consumption (NU) of all seedlings in the certain period mi ) The calculation formula is as follows:
NU mi =E mi +C mi-1 -R mi -C mi (6)
said E mi And R mi The calculation formula of (2) is as follows:
E mi =c m1 ·V,R mi =c m2 ·D (7)
in equations (6) and (7), NU mi : the nutrient consumption of all the seedlings in a certain period of time is unit g, c mi1 And c mi2 The nutrient concentration in unit g/L of the concentrated nutrient solution and the nutrient concentration in the lost water are respectively; v and D are respectively the dosage of the concentrated nutrient solution and the loss water in a certain time period, and the unit is L; c mi-1 : is the residual amount of nutrients in the matrix at the beginning of a certain time period, in g; c mi : is the residual amount of nutrients in the matrix at the end of a certain time period, in g; e mi And R mi Respectively the nutrient application amount in a certain time periodAnd loss in g; v and D are respectively the dosage of the concentrated nutrient solution and the water loss in a certain time period, and the unit L is; c. C m1 And c m2 The nutrient concentration in the concentrated nutrient solution and the nutrient concentration in the lost water are respectively expressed in unit g/L;
the water storage capacity of the substrate is calculated by the formula:
MWS i =a·b·[(θ j ·l+θ nj ·(h-l)]·ρ 1 ·10 -3 (8)
the matrix nutrient residual quantity calculation formula is as follows: comprises the following steps:
C mi =a·b·ρ 2 [(N m-j ·l+N m-nj ·(h-l)]·10 -6 (9)
in the formulae (8) and (9), θ j : monitoring the water content of the matrix volume of the layer for a plan; theta.theta. nj : average volume water content of the substrate of the unplanned monitoring layer; n is a radical of m-j : for planned monitoring of the nutrient content of the layer substrate, the unit mg.kg -1 ;N m-nj : the average nutrient content of the substrate of the unplanned wet layer is in mg.kg -1 (ii) a a: the length of the planting area is unit cm; b: the width of the planting area is unit cm; l: is the sensor probe length in cm; rho 1 And ρ 2 Respectively the density of water in the matrix and the volume weight of the matrix in g cm -3 (ii) a h: the thickness of the matrix layer is in cm;
further, if there is an increase in irrigation and fertilization within a statistical time period, the MWS in the formulas (3) and (6) i-1 And C mi-1 To monitor the water storage capacity (MWS) of the substrate when the water and nutrient contents of the substrate reach upper limits upper ) And residual amount of nutrients (C) m-upper ) (ii) a The irrigation quantity is obtained by adopting an irrigation electromagnetic flowmeter, the water loss quantity is obtained by adopting an electronic scale with the measuring range of 3kg and the precision of 0.01g for weighing, and the dosage of the concentrated nutrient solution is obtained by adopting a nutrient electromagnetic flowmeter;
b, constructing a multi-objective comprehensive decision model to optimize and find out the optimal water and fertilizer combination of the pinus massoniana seeding seedlings in different growth stages, and adopting the following steps;
a. based on the principle of partial least square method, establishing a multivariate quadratic regression model by taking the water consumption, the nitrogen, phosphorus and potassium absorption and the height-diameter ratio which are required to be consumed and absorbed by increasing 1g of dry matter every time in each growth and development stage as response variables and the applied water, nitrogen, phosphorus and potassium as independent variables:
in formula (9): y is n-m : as response variable, m: increasing the water consumption, the absorbed nitrogen, phosphorus and potassium amount or the height-diameter ratio required for forming 1g of dry matter for each accumulation in each growth and development stage; θ: volume water content of matrix, N: is nitrogen content, P: phosphorus content, K: is the potassium content, b m0 、b m1 、b m2 、b m3 .....b m14 : is a constant term; n: for each growth and development stage;
b. determining the respective response variable y n-m The formula is:
in the formula, w m : as a weight, λ m : is the variance;
c. calculating the optimal predicted value of each response variable, then minimizing the weighted residual square sum of each response variable and the optimal predicted value, and establishing a comprehensive multi-objective decision model:
w m : is a weight value, y n-m : is a quadratic equation of each response variable, OA n-m : the optimal predicted value of each response variable is obtained;
d. optimizing the comprehensive multi-target decision model to obtain an optimal water and fertilizer combination for realizing coordinated and efficient absorption of water and nutrients in different growth and development stages of the nursery stock;
further, in the step C, the optimal water and fertilizer management adopts an accurate water and fertilizer regulation and control system to respectively regulate and control the optimal water and fertilizer combinations of the pinus massoniana seeding seedlings in different growth and development stages to ensure that the water and nutrient contents in the matrix of the root zone of the seedlings in different growth and development stages are kept in an optimal water and fertilizer range in real time, and the amount of water and fertilizer, the evaporation amount of water, the loss amount of water and nutrient, and the water storage amount and the residual amount of nutrient in the matrix irrigated in the time period are regularly counted;
further, calculating the water consumption and the absorbed nutrient amount of the nursery stock growing and developing under the regulation and control of the optimal water and fertilizer by adopting formulas (3) - (9) in the formula A;
further, a dynamic curve chart of water consumption and fertilizer demand of the pinus massoniana seedlings in different growth and development stages is drawn by taking the sowing date as an abscissa and taking the water consumption and the nutrient absorption as an ordinate.
The invention has the beneficial effects that:
(1) according to the distribution characteristics of the roots of the pinus massoniana seeding seedlings in the matrix along with the growth process, a water and fertilizer precise regulation and control system is adopted to carry out space-time dynamic positioning, quantitative and timely water and fertilizer supplement on the water and the nutrients in the root area of the seedlings, so that the water and nutrient contents in the matrix of the root area of the seedlings in different growth and development stages are kept within a target threshold range in real time, and the good control of the water and the nutrients in the growth environment of the seedlings is realized;
(2) through orthogonal water and fertilizer tests, a multi-target comprehensive decision model is constructed to solve the optimal water and fertilizer combination of the nursery stock for efficiently absorbing the water and fertilizer in different growth and development stages, the optimal water and fertilizer combination of the nursery stock in different growth and development stages is regulated and controlled in different growth and development stages of the nursery stock respectively, the source and the destination of the water and fertilizer are carefully mastered by regularly counting the irrigation amount and the fertilization amount, the water evaporation amount, the water and nutrient loss amount, and the water storage amount and the nutrient residue condition in a matrix in different growth and development stages of the nursery stock, and the absorption rule and the absorption amount of the water and fertilizer in different growth and development stages and different growth and development stages in the same growth and development stage of the nursery stock are accurately mastered.
Drawings
FIG. 1 is a flow chart of the water and fertilizer requirement rules and the water and fertilizer requirement quantity of masson pine seeding seedlings in different growth and development stages;
FIG. 2 is a schematic diagram showing the absorption rule of nitrogen, phosphorus and potassium in the early growth stage of masson pine seedlings;
Detailed Description
The present invention is further illustrated by the following examples, which are provided for the purpose of illustration only and are not intended to limit the scope of the present invention.
As shown in fig. 1, in this embodiment, to obtain the law of water and fertilizer demand and its demand in different growth and development stages of pinus massoniana seeding, a water and fertilizer coupling regulation and control system is used to precisely control the supply of water and fertilizer, so as to ensure that the water and nutrients in the root zone matrix of the different growth and development stages of the seeding are kept in the control range in real time, the irrigation and fertilization amounts, the water evaporation amount, the water and nutrient loss amount, the water and water storage amount and nutrient residue in the matrix in each growth and development stage are counted, an optimal water and fertilizer combination is obtained by constructing a multi-objective comprehensive decision model to solve the optimal water and fertilizer combination when the amount of water, nitrogen, phosphorus and potassium consumed by increasing 1g of dry matter for each accumulation in different growth and development stages of the seeding is optimized, and the irrigation and fertilization amounts, the water and fertilizer consumption in the period, and the water and fertilizer consumption amount in each interval are counted, Water evaporation amount, water and nutrient loss amount, water storage amount in the matrix and nutrient residue condition, and calculating water consumption and absorbed nitrogen, phosphorus and potassium amount required by seedling growth in different growth stages and different growth periods in the same growth stage;
the accurate water and fertilizer supply device is provided with a water and nutrient sensor through each seedling raising device and is used for monitoring water and nutrient information in each seedling raising matrix and sending the information to a data acquisition module corresponding to an integrated control center; the integrated control center comprises an integrated control cabinet and a water and fertilizer control platform and is used for analyzing and judging whether watering and fertilizing are performed or not and generating a corresponding water and fertilizer supply scheme to keep the water and the nutrients in the seedling root area matrix within a control range in real time based on the water and nutrient information in the matrix of the seedling root area in each different water and fertilizer supply seedling unit and comparing the preset water and nutrient upper and lower limit values of the different seedling unit, so that the consistency of the seedling growth environment is ensured.
Carrying out an accurate water and fertilizer management test of the masson pine seeding: selecting pinus massoniana seedlings with uniform growth vigor, wherein the shells of the pinus massoniana seedlings are not dropped and the main roots of the pinus massoniana seedlings are cut off, transplanting the pinus massoniana seedlings into a seedling culture groove with the length (a) being 3m, the width (b) being 70cm and the height being 30cm, filling a mixed matrix of quartz sand, perlite, vermiculite and yellow core soil into the seedling culture groove, wherein the yellow core soil accounts for 5 percent of the mixed weight of the quartz sand, the perlite and the vermiculite according to the volume ratio of 3:2:4, the thickness (h) of the matrix in the seedling culture groove after being compacted is 25cm, and the volume weight of the matrix is 1.08g/cm 3 Water content by volume in field (. theta.) max ) It was 44.16%. The plant spacing and the row spacing of the seedlings in each seedling raising unit are both 8cm, and the total number of the seedlings is 342. An evaporator with the inner diameter (r) of 8cm, the height of 25cm and the bottom of 3 drain holes for measuring the water evaporation amount is respectively embedded between the two ends of the seedling culture groove and the line space of the middle position, a substrate which is the same as the planted seedlings is arranged in the evaporator, the plane of the substrate in the evaporator is flush with the plane of the substrate in the seedling culture groove, and an electronic balance with the precision of 0.01g is adopted for weighing every day and the weight (M) is recorded;
the water and fertilizer management sets 4 factors of moisture (W), nitrogen (N), phosphorus (P) and potassium (K), the moisture treatment sets 4 levels of full irrigation, light deficit irrigation, medium deficit irrigation and heavy deficit irrigation, and the fertilization sets 16 different water and fertilizer coupling combinations of four fertilization levels of high fertilizer, medium fertilizer and low fertilizer. Ca (NO) for nitrogen, phosphorus and potassium 3 ) 2 、NH 4 Cl、NH 4 H 2 PO 4 、KNO 3 And KCl, are provided and are all analytically pure.
The following embodiments take the initial stage and the fast-growing stage of seedling growth as examples, and introduce a method for dynamically monitoring the moisture content and the nitrogen, phosphorus and potassium content in a seedling root zone matrix in time and space along with the growth process of seedlings in the seedling growing process by a moisture and nutrient sensor, performing precise difference water replenishing and fertilizer replenishing, and ensuring that the moisture content and the nutrient content in the seedling root zone are kept within the target threshold value range in real time
The seedling growth initial stage is also called the rooting stage: the method means that 90% of seed cases of pinus massoniana seedlings drop off after emergence until the growth speed of the seedlings is greatly increased, and the growth characteristics of the seedlings at the period are that the root system grows fast and the overground part grows slowly. The monitoring range of the sensor is the water and nutrient information in the cylinder with the probe as the center and the length of the probe as the diameter. As shown in the schematic diagram of the position change of the water sensor 11 and the nutrient sensor 12 in fig. 1, the early root system of the seedling in the early growth stage is distributed on a matrix layer of about 2-5cm, the water sensor and the nitrogen, phosphorus and potassium sensor probe monitor the water content and the nitrogen, phosphorus and potassium content of the matrix layer which are about 1-8cm downwards based on the horizontal plane of the matrix, along with the progress of the growth process, the later root system distribution depth of the seedling in the early growth stage is about 10-15cm, the water sensor and the nitrogen, phosphorus and potassium sensor are moved downwards by about 7cm, and the water content and the nitrogen, phosphorus and potassium content of the matrix layer of 8-15cm are monitored. When the seedling enters the middle period of the fast growth period, the root system is mainly distributed at a depth of 15-25cm, the sensor is moved by about 7cm at the moment, the moisture and the nitrogen, phosphorus and potassium contents of the matrix layer of 15-22cm are monitored, and the sensor can be kept at the depth of 15-22cm from the middle period of the fast growth period to the position of the sensor in the dormancy period;
TABLE 1L 16 (4 4 ) Water and fertilizer management meter
In table 1, the upper and lower limit values of water management and nitrogen, phosphorus and potassium fertilizer management represent target threshold ranges of water and nitrogen, phosphorus and potassium, and when the monitored value is lower than the lower limit value, a control program is triggered to automatically calculate the amount of water and fertilizer required to be output to supplement the target threshold upper limit value and output the corresponding amount of water and fertilizer to perform difference water supplement or fertilizer supplement; theta.theta. max The maximum volume water content of the matrix is 44.16 percent;
the growth speeds of the nursery stocks treated by different water and fertilizer are different, the embedding depth of the sensor can be adjusted according to the distribution characteristics of the root system, and the positions of the irrigator and the sensor are basically consistent;
selecting 30 seedlings with consistent growth vigor according to the judgment standard of the seedling growth process, measuring the height of each seedling every 10 days, considering that the seedling enters the fast growth period from the beginning of the growth period when the seedling growth amounts measured in two adjacent times are remarkably increased, considering that the seedling enters the late growth period from the end of the fast growth period when the seedling growth amounts measured in two adjacent times are remarkably reduced, and considering that the seedling enters the dormancy period from the end of the late growth period when the seedling growth amounts measured in two adjacent times are not substantially increased.
The following examples describe the amount of water and nitrogen applied, the amount of water and nitrogen lost, the amount of water stored in a substrate and the amount of nitrogen left during the growth of the nursery stock in the period of time which is from the beginning to the end of the growth period of the pinus massoniana nursery stock treated by T1; weighing method is adopted every day to calculate water evaporation amount within 24h, and water consumption and nitrogen absorption amount required for each accumulation increase to form 1g dry matter in the early growth stage of nursery stock are calculated
Before the test is started, 0-25cm layers of matrix are taken out and uniformly mixed at two ends and the middle position of a seedling culture groove by adopting a sampler with the minimum scale of 1cm respectively by adopting a five-point method, the mixture is placed into a container and compacted, and a water content sensor and a nutrient sensor probe are completely inserted into the container until the water content (theta) is stable 0 ) A reading of 30.15% and a nitrogen content reading of 259mg/kg, water storage MWS in the matrix T1-0 =a×b×ρ 1 ×θ j ×h×10 -3 =300×70×1×10 -3 X 0.3015X 25 ═ 158.288L, and the nitrogen content in the matrix is C T1-N-0 =a×b×ρ 2 ×θ j ×h×10 -6 =300×70×1.08×259×25×10 -6 146.853g, formula a: the length of the planting area is unit cm; b: the width of the planting area is in unit cm; h: the thickness of the matrix layer is in cm; rho 1 And ρ 1 Respectively representing the density of water and the volume weight of a matrix; resetting all the electromagnetic flow meters and removing liquid in the water receiving tank and the recovery barrel;
when the seedling starts to grow in the initial stage, the root system of the seedling is mainly distributed on a substrate layer of about 2-5cm, so that the positions of probes of the water sensor and the nitrogen, phosphorus and potassium sensor are downward 1-8cm of the substrate layer by taking the horizontal plane of the substrate as a reference, the measured water volume and the water content are 30.37 percent and are lower than the lower limit value of the set target threshold value by 41.07 percent; the nitrogen content is 245.15mg/kg and is in the range of the target threshold value, and the water and fertilizer control program is triggered to automatically calculate the output required by complementing the nitrogen content to the upper limit value of the target threshold valueCarrying out differential water replenishing on the water quantity; when the nitrogen content in the monitoring layer matrix is reduced to have an error of 0.1% with a target lower limit value 239mg/kg of nitrogen content, taking out and discarding the matrix of the layer of 0-8cm by using a sampler, taking out the matrix of the layer of 8-25cm, fully mixing, filling into a container, reading the detected nitrogen content to be 241.12mg/kg, and determining that the nitrogen residue in the matrix is C when the nitrogen content of the layer of 1-8cm is the target lower limit value of the threshold value T1-N-lower =a·b·ρ 2 [(N m-j ·l+N m-nj ·(h-l)]·10 -6 =300×70×1.08×[(239×7+241.12×(25-7)]×10 -6 =136.378g;
After the test, the evaporator is weighed with an electronic balance with an accuracy of 0.01g each day and the weight (M) is recorded, if the weight of the evaporator is M for two times within 24h, the weight is M 1 790.45g and M 2 778.92g, the water evaporation rate of the seedling raising field in 24h isIn the formula, M 1 And M 2 Is the total weight (g) at time t1 and t 2; a: area (cm) of evaporator inner tube 2 ) (ii) a Δ t: the time interval (h) of two adjacent weighing times; evr: evaporation rate in g cm -2 ·h -1 ;
Sensor monitoring area (theta) at the end of seedling growth period j ) The water content (theta) of the matrix layer is 41.95 percent, the nitrogen content is 250mg/kg, and the water filling electromagnetic flowmeter is I T1-original 69.118L, a measuring cylinder and an electronic scale with the measuring range of 3kg and the precision of 0.01g are adopted to obtain the water shunt loss D T1-original 32.43kg, nutrient electromagnetic flowmeter reading V T1-original 5.95L, nutrient liquid nitrogen concentration c Ni Is 25.2 g.L -1 Total water evaporation amount Z in the initial stage of growth T1-original =S·∑Δt·Evr i When the plant is 11.475L, the dry matter of each seedling is increased by 0.061g on average, and the nitrogen fertilizer application amount E is applied in the early growth stage of the seedling T1-N-primary =c N1 ×V=25.2g/L×5.95L=149.94g;
Taking out the matrix by layering with a sampler, discarding 7-14cm layer of matrix, mixing 0-7cm layer of matrix and 15-22cm layer of matrix, placing into a container, compacting, and adding waterSensor and nutrient sensor probe are inserted into the unplanned monitoring layer (theta) after the value is stable nj ) The volume water content reading is 43.05 percent, the nitrogen content reading is 258mg/kg, and then the water storage quantity MWS in the matrix T1-original =a·b·ρ 1 ×10 -3 [θ j ×l+θ nj ·(h-l)]=300×70×1×10 -3 ×[0.4195×7+0.4305×(25-7)]224.396L, residual nitrogen content C T1-N-primary =a×b×ρ 1 ×[(N j ×l+N nj ×(h-l))]×10 -6 =300×70×1.08×[(250×7+258×(25-7))]×10 -6 145.016 g. In the formula, theta j : monitoring the water content of the matrix volume of the layer for a plan; theta nj : average volume water content of the substrate of the unplanned monitoring layer; l: the length of a probe of the moisture sensor is in cm; rho 1 And ρ 1 Respectively representing the density of water and the volume weight of the matrix in g cm -3 ;
After moving to a substrate layer of 7-14cm under a sensor at the early stage of seedling growth, measuring the water storage MWS in the substrate when the water supplementing and the nitrogen supplementing fertilizers in the monitoring area reach the upper limit value in a time-selective manner upper Are 230.29L and C N-upper 152.704 g;
the nitrogen loss is measured by sodium salicylate-hydrazine sulfate through a full-automatic chemical analyzer to obtain the nitrogen concentration c in the lost 32.430L water N2 Is 0.127 g.L -1 The nitrogen loss is R T1-N-primary =c N2 ×D=0.127g/L×32.43L=4.12g;
The amount of water consumed for every 1g of dry matter accumulated in the growth stage of the pinus massoniana seedling is increased
The following embodiments introduce the construction of a multi-objective comprehensive decision model to solve the optimal water-fertilizer combination when the water consumption, the nitrogen, phosphorus and potassium consumption and the aspect ratio which are required to increase 1g of dry matter per accumulation are optimal at the initial growth stage of the pinus massoniana seedling, and the concrete steps are as follows:
a, constructing a multivariate quadratic regression model of water consumption, nitrogen, phosphorus and potassium consumption and height-diameter ratio, irrigation and nitrogen, phosphorus and potassium which are required to be consumed by increasing 1g dry matter every time the masson pine seeding seedlings are accumulated in the initial growth stage based on a least-squares multiplication principle;
TABLE 2 multivariate quadratic equation of water consumption, NPK amount and height-diameter ratio for increasing 1g dry matter formation in early growth stage of nursery stock
b, the weight of the water consumption, the nitrogen phosphorus potassium quantity and the height-diameter ratio which are required for increasing and forming 1g of dry matter in each accumulation of the pinus massoniana seedlings in the whole growth initial stage are respectively as follows: the water demand is 0.2178, the height-diameter ratio is 0.2482, the N consumption is 0.1392, the P consumption is 0.2073, and the K consumption is 0.1876;
c. calculating the optimal predicted value of each response variable by ASA software, then minimizing the weighted residual sum of squares of each response variable and the optimal predicted value,in the formula w m : is a weight value of y n-m : is a quadratic equation of each response variable, OA n-m : the optimal predicted value of each response variable is obtained;
within the scope of the test, it is required to increase the amount of water Y consumed per cumulative increase in the formation of 1g of dry matter 1 And aspect ratio Y 5 Taking the minimum value, the absorption of nitrogen Y required per cumulative increase to form 1g of dry matter 2 Phosphorus Y 3 Potassium Y 4 Measuring the maximum value with the constraint condition of W35, 100]、N[42,256]、P[6,37]、K[47,280]Any value in the above steps, so as to obtain a comprehensive multi-target decision model of the pinus massoniana seedling in the initial growth stage
min Early stage of growth =0.2178×(Y 1 +1445.848) 2 +0.2482×(Y 2 -301.9248) 2 +0.1392×(Y 3 -295.0473) 2 +0.2073×(Y 4 -761.1667) 2 +0.1876×(Y 5 -22.54575) 2 g·cm -2 ;
d. Optimizing the model by using an optimization software LINGO to obtain the optimal water and fertilizer combination which is beneficial to the growth of the nursery stock in the initial growth stage of the pinus massoniana sown seedling and can promote the nursery stock to absorb and utilize water and nutrients, wherein the optimal water and fertilizer combination has the volume water content of 73.214 percent of the field volume water content and the nitrogen content of 80.34mg kg -1 Phosphorus content of 37 mg/kg -1 The potassium content is 262.109 mg/kg -1 。
The following introduces and develops the optimum water and fertilizer regulation and control treatment test of the pinus massoniana seeding seedling at the initial growth stage
The seedling raising and data collection are the same as the development of the accurate water and fertilizer coupling management test of the pinus massoniana sown seedlings, and the water consumption and the absorbed nutrient amount of the seedlings growing under the optimal water and fertilizer regulation are calculated by adopting the formulas (3) - (9) in the formula A. And regulating and controlling the optimal water and fertilizer combination which is solved according to the multi-objective comprehensive decision model and can promote the pinus massoniana seeding seedling to efficiently absorb water and nutrients in the early growth stage. Setting the water content of 73.214 +/-2% and the nitrogen content of 80.34 +/-2% mg/kg in the water and fertilizer parameter area of the water and fertilizer control platform -1 Phosphorus content of 37 +/-2% mg/kg -1 The potassium content is 262.109 +/-2% mg/kg -1 When the seedling grows to the fast growing stage, inputting corresponding parameters;
as can be seen from figure 2, the nutrient demand in the early stage of the seedling growth stage is less, but with the progress of growth, the demand for nitrogen fertilizer is gradually increased, the demand for phosphate fertilizer is reduced after the peak value is reached in the middle stage of the growth stage, and the demand for potassium fertilizer is obviously increased until the seedling grows to the later stage. Therefore, in the seedling raising process, the phosphate fertilizer can be properly applied in the middle stage of the seedling growth initial stage, and the potassium fertilizer is applied in the later stage of the seedling growth, so that the growth and development of the seedling are facilitated, and the waste of water and fertilizer and the pollution to the environment caused by high water and high fertilizer content are avoided.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can substitute or change the technical solution of the present invention and the inventive concept within the technical scope of the present invention.
Claims (5)
1. A method for calculating a water and fertilizer requirement rule of masson pine seeding based on water and fertilizer regulation is characterized by comprising the following steps:
a, calculating the consumed water amount and the consumed nutrient amount required by increasing 1g of dry matter per each single seedling in different growth and development stages of the pinus massoniana sown seedlings under different water and fertilizer treatments through a water and fertilizer coupling test;
b, constructing a multi-target comprehensive decision model to optimally find out the optimal water and fertilizer combination for the nursery stock to efficiently absorb water and fertilizer at different growth and development stages;
c, respectively regulating and controlling the optimal water and fertilizer combinations of the pinus massoniana seeding seedlings in different growth and development stages to be in different growth and development stages of the seedlings through the optimization of the multi-target comprehensive decision model, and calculating the water and fertilizer requirements and the water and fertilizer requirements of the seedlings in different growth and development stages and different growth stages in the same growth and development stage under the optimal water and fertilizer conditions;
wherein the content of the first and second substances,
b, performing space-time dynamic accurate control on water and fertilizer supply amount on water and nutrients in a root zone of the nursery stock by adopting an accurate water and fertilizer regulation and control system in the water and fertilizer coupling test process in the step A, and ensuring that the water and nutrient content in a matrix of the root zone of the nursery stock at different growth and development stages is kept within a target threshold range in real time;
in the step A, calculating and recording the water storage amount in the seedling culture substrate treated by different water fertilizers on the same sowing day) And residual amount of nutrients: () (ii) a After sowing, the amount of water irrigated during the time period for each treatment was periodically counted () Nutrient applicationAmount (A)) Water loss amount () And nutrient loss amount () Amount of water evaporated () Water storage capacity in the matrix: () And residual amount of nutrients: (),: is a treatment number;: nitrogen, phosphorus and potassium;: is a certain period of time;
in the step A, the water consumption required by increasing 1g of dry matter per each single seedling in different growth and development stages of the pinus massoniana sown seedlings under different water and fertilizer treatments is calculated () And the amount of nutrients to be absorbed: () The calculation formulas are respectively as follows:
in the formulas (1) and (2),andrespectively increasing the amount of nutrients required to be absorbed and the amount of water required to be consumed by 1g of dry matters for each seedling in each growth and development stage;andrespectively the nutrient consumption and the water consumption of all the seedlings in a certain time period in each growth and development stage,unit,Unit of;And: respectively the total dry weight of the whole plants of a certain number of nursery stocks in unit g at the end of the last growth and development stage and the end of the growth and development stage;: at different growth and development stages;: the total number of the seedlings in each growth and development stage;
for a certain period of time (1) and (2) of said formulae) Water consumption of all seedlings: () And nutrient consumption: () The calculation formulas are respectively as follows:
in the formulas (3) and (4),and: the water consumption and nutrient consumption of all the seedlings in a certain time period,unit;Has a unit of;: is the irrigation quantity in a certain time period;: is the amount of water lost in a certain period of time, unit;: the water evaporation amount of the seedling culture land in a certain time period; unit of;: the water storage capacity in the seedling substrate at the end of a certain time period is unit;: the water storage capacity in the seedling substrate at the beginning of a certain time period is unit;Andrespectively the nutrient application amount and the loss amount in a certain time period;: the residual amount of nutrients in the matrix at the beginning of a certain time period is expressed in units;: the residual amount of nutrients in the matrix at the end of a certain period of time, unit。
2. The method for calculating the water and fertilizer requirement rule of the masson pine seeding based on the water and fertilizer regulation and control as claimed in claim 1, wherein the water storage capacity and the nutrient residual quantity of the matrix in the formulas (3) and (4) are calculated by the following formulas:
in the equations (5) and (6),: monitoring the water content of the matrix volume of the layer for a plan;: the average volume water content of the substrate of the unplanned monitoring layer is obtained;: monitoring the nutrient content of the layer substrate for planning;: is the average nutrient content of the substrate of the unplanned wetting layer in unit;: is the length of the planting area in unit;: is the width of the planting area in unit;: is the sensor probe length, unit;Andrespectively the density of water in the matrix and the volume weight of the matrix in units;: is the thickness of the substrate layer, unit。
3. The method for calculating the water and fertilizer requirement law of masson pine sowed seedlings based on water and fertilizer regulation and control as claimed in claim 1, wherein the water evaporation capacity of the seedling raising field in a certain time period in the formula (3) (water and fertilizer requirement law)) The calculation formula is as follows:
the above-mentionedIs the evaporation rate of the seedling raising land per unit areaThe calculation formula is as follows:
4. The method for calculating the water and fertilizer requirement law of the masson pine seeding based on water and fertilizer regulation and control as claimed in claim 1, wherein in the formulas (3) and (4), if irrigation and fertilization are increased within a statistical time period,andfor monitoring the water content and nutrient content of the layer matrix, the water storage capacity in the matrix is up to the upper limit value) And residual amount of nutrients: () (ii) a In the above formula (4)Andare respectively calculated as,(ii) a In the formulaAndthe dosage of the concentrated nutrient solution and the water loss in a certain time period are respectively unit;Andthe nutrient concentration in the concentrated nutrient solution and the loss water is respectively unit。
5. The method for calculating the water and fertilizer requirement rule of the masson pine seeding based on water and fertilizer regulation and control as claimed in claim 1, wherein the method for constructing the solution of the multi-objective comprehensive decision model comprises the following steps:
a. based on the principle of partial least square method, establishing a multivariate quadratic regression model by taking the water consumption, the absorbed nitrogen, phosphorus and potassium amount and the height-diameter ratio which are required to be consumed for increasing 1g of dry matters in each growth and development stage as response variables and the applied water, the applied nitrogen, the phosphorus and the potassium as independent variables:
in the formula:: in response to the variable(s) being varied,: increasing the water consumption, the absorbed nitrogen, phosphorus and potassium amount or the height-diameter ratio required for forming 1g of dry matter for each accumulation in each growth and development stage;: is the water content of the matrix by volume,: in order to be the nitrogen content,: in order to be the content of phosphorus,: in terms of the content of potassium, the amount of potassium,: is a constant term;
in the formula (I), the compound is shown in the specification,: in order to be the weight of the weight,: is the variance;
c. calculating the optimal predicted value of each response variable, then minimizing the weighted residual square sum of each response variable and the optimal predicted value, and establishing a comprehensive multi-objective decision model:
: is a weight value of the weight value,: is a quadratic equation for each of the response variables,: the optimal predicted value of each response variable is obtained;
d. and optimizing the comprehensive multi-objective decision model to obtain the optimal water and fertilizer combination for realizing coordinated and efficient absorption of water and nutrients in different growth and development stages of the nursery stock.
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