CN114514925B - Full-automatic low temperature storage equipment of stem cell - Google Patents
Full-automatic low temperature storage equipment of stem cell Download PDFInfo
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- CN114514925B CN114514925B CN202210304279.7A CN202210304279A CN114514925B CN 114514925 B CN114514925 B CN 114514925B CN 202210304279 A CN202210304279 A CN 202210304279A CN 114514925 B CN114514925 B CN 114514925B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
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Abstract
The invention discloses full-automatic low-temperature storage equipment for stem cells, and relates to the technical field of low-temperature storage. This full-automatic low temperature storage equipment of stem cell, including the heat sink, the heat sink includes the cooling shell, the inside center department of cooling shell is provided with placer, placer is including placing the shell, the last port of placing the shell flushes with the last port of cooling shell, the lower extreme of placing the shell is in the top of the lower extreme of cooling shell, the bottom opening has been seted up in the middle part of the lower extreme of placing the shell, the side opening has all been seted up in the middle part left and right sides wall of placing the shell in the side wall of running through, the inside lower extreme of cooling shell is provided with the intercommunication device, the intercommunication device includes the sill pillar, the lower extreme fixed connection of sill pillar is the lower inner wall middle part of cooling shell, the upper end activity of sill pillar has cup jointed the upper end, the upper end of upper pillar passes the bottom opening and with the bottom opening adaptation, this full-automatic low temperature storage equipment of stem cell, the staff can not contact the temperature of nitrogen gas for the test tube that is equipped with the stem cell can be taken out safely.
Description
Technical Field
The invention relates to the technical field of low-temperature storage, in particular to full-automatic low-temperature storage equipment for stem cells.
Background
In general, there is a population of intermediate progenitor cells, known as "committed progenitor cells", between stem cells and their terminally differentiated daughter cells, which have limited expansion capacity and limited differentiation potential. The function of these cell populations is to increase the number of differentiated cells that are produced after each division of stem cells. Stem cells have the ability to self-renew, but stem cell division is relatively asymmetric in nature. Functionally, stem cells are cells with multipotent differentiation potential and self-renewal capacity, the most primitive cells at the top of the cell line origin, and are capable of differentiating in vivo to produce a specific tissue type. The stem cells lose self-renewal capacity once they differentiate into progenitor cells, symmetrical mitosis occurs, the number of progenitor cells is only supplemented by proliferation and differentiation of stem cells, but progenitor cells still maintain a high proliferation capacity, and in the case of hematopoietic stem progenitor cells, the massive expansion of cells in hematopoietic processes is mainly dependent on the proliferation of hematopoietic progenitor cells. In adult organs, stem cells can repair tissue by constantly dividing, or be in a quiescent state as in mammalian brain tissue. Stem cells can self-renew and produce more progenitor cells of different differentiation types during their development by dividing symmetrically to expand their number, or by dividing asymmetrically.
The stem cells are separated and cultured from different human tissues, then detected and identified, and then frozen in low temperature, so that the stem cells can be recovered for reinfusion of patients in clinical needs, and the aim of treating diseases is fulfilled. Therefore, it is necessary to preserve stem cells using a low-temperature storage device.
At present, most stem cells in the market need to be preserved at low temperature, and test tubes with stem cells are generally put into a box filled with nitrogen for uniform preservation, and when the test tubes are taken out from the box every time, nitrogen can overflow from the box mouth in a large amount, so that other test tubes with stem cells in the box are influenced, and meanwhile, the nitrogen overflowed from the box mouth can frostbite staff, so that the stem cells are quite unsafe, and therefore, a full-automatic low-temperature storage device for stem cells is needed to solve the problems.
Disclosure of Invention
The invention aims to provide a full-automatic low-temperature storage device for stem cells, so as to solve the problems in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a full-automatic low temperature storage equipment of stem cell, includes the heat sink, the heat sink includes the cooling shell, the inside center department of cooling shell is provided with placer, placer is including placing the shell, the last port of placing the shell flushes with the last port of cooling shell, the lower extreme of placing the shell is in the top of the lower extreme of cooling shell, the lower extreme middle part of placing the shell has run through and has seted up the bottom opening, the lateral wall has all run through in the middle part of placing the shell has been seted up the side opening, the inside lower extreme of cooling shell is provided with intercommunication device, intercommunication device includes the sill pillar, the lower extreme fixed connection cooling shell's of sill pillar lower inner wall middle part, the upper end activity of sill pillar has cup jointed the spliced pole, the upper end of spliced pole passes the bottom opening and with the sill opening adaptation, the upper end of sill pillar stretches into the inside of placing the shell, the upper end fixedly connected with sill bowl of spliced pole, the sill bowl presents the form, the lower lateral wall fixedly connected with spring one of sill bowl, the lower extreme fixedly connected with of spring one, the lower extreme fixedly connected with of sill is placed on the inner wall of the sill pillar, the upper side of the sill is connected with the outside.
Preferably, a nitrogen valve is fixedly arranged at the upper part of the front side of the cooling shell, and the nitrogen valve is communicated with the inside of the cooling shell.
Preferably, the outside of going up the post presents annular buckling, the lower extreme of going up the post is in the below of placing the shell, the left and right sides of going up the post all meshes there is gear one, the middle part through connection of gear one has gear shaft one, gear shaft one passes through the bearing rotation with gear one and is connected, the inside wall of both ends fixed connection cooling shell around gear shaft one, gear one has gear two in the meshing of the one side of keeping away from the post, the middle part through connection of gear two has gear shaft two, gear shaft two passes through the bearing with gear two and rotates to be connected, the inside wall of both ends fixed connection cooling shell around the gear shaft two.
Preferably, the front and back surfaces of the second gear are fixedly connected with connecting rings, the connecting rings are sleeved on the outer side of the second gear, the front side wall and the back side wall of the connecting rings are connected with connecting plates jointly, the connecting plates are arc-shaped, the connecting plates are fixedly connected with plugging plates at the ends far away from the connecting rings, the plugging plates are correspondingly inserted into the side holes and are matched with the side holes, the plugging plates are arc-shaped, the lower ends of the plugging plates are contacted with the lower inner walls of the side holes, the upper ends of the plugging plates penetrate through the side holes and extend into the inner part of the placing shell, the upper ends of the plugging plates are fixedly connected with an air bag I, the lower ends of the plugging plates are fixedly connected with an air bag II, the edges of the upper parts of the plugging plates are fixedly connected with plugging strips, the plugging strips are elastic, the whole plugging strips are U-shaped, the ends of the plugging strips far away from the plugging plates penetrate through the inner walls of the cooling shell, the side holes are correspondingly connected with the side holes, the plugging strips are positioned above the ends connected with the side holes, and the air valve is positioned above the plugging strips.
Preferably, the upper port of cooling shell and nitrogen valve is provided with roof device jointly, roof device includes the roof, the roof presents the annular, the outer lateral wall of shell is placed in the inner circle fixed connection of roof, the inner wall of outer lane fixed connection cooling shell of roof, gas pocket one has all been seted up to the upper surface left and right sides of roof, the inside embedding fixedly connected with cross opening pad of gas pocket one.
Preferably, the top of cooling shell and nitrogen valve is provided with the device of taking, the device of taking is including taking the shell, the shell of taking presents the upper hemispherical shell form, the shell of taking is the same with the diameter of cooling shell, the inside center department of the shell of taking is provided with the inner shell, the inner shell presents the semi-cylindrical shell form, the inner shell is the same with the diameter of placing the shell, the lower port of shell and inner shell of taking flushes, the clamping groove has all been seted up about the inside wall of inner shell, two clamping groove symmetry, clamping groove's inside embedding is connected with the clamping, clamping and clamping groove adaptation, two the side middle part fixedly connected with post one that the clamping deviates from, the spring two has been cup jointed in the one end outside that is close to the clamping to the post one end, the one end fixed connection clamping and the other end fixed connection clamping groove of spring two.
Preferably, the first end of the post far away from the clamping penetrates through the side wall of the inner shell and is fixedly connected with a movable barrel, the movable barrel is located between the taking shell and the inner shell, the movable barrel is in a horizontal cylindrical shell shape, the second end face of the movable barrel far away from the first post penetrates through and is sleeved with the first post, the first post is inserted into the movable barrel at the end close to the movable barrel and is fixedly connected with the first plate, the first plate is fixedly connected with the third spring at the end far away from the first post, and the third spring is fixedly connected with the inner wall of the movable barrel at the end far away from the first plate.
Preferably, the bottom groove is formed in the end, close to the second column, of the lower side wall of the movable barrel, a sliding block is fixedly connected to the lower side wall of the first plate, the sliding block is inserted into the bottom groove, a sliding rod is fixedly connected to the lower end of the sliding block, and the second column penetrates through the side wall of the taking shell at the end, far away from the movable barrel, and is fixedly connected with a handle.
Preferably, the bottom plate device comprises a bottom plate, the bottom plate is annular, the inner ring of the bottom plate is fixedly connected with the outer side wall of the bottom port of the inner shell, the outer ring of the bottom plate is fixedly connected with the inner side wall of the bottom port of the taking shell, air holes II are formed in the left side and the right side of the surface of the bottom plate, the air holes II are opposite to the air holes I, side grooves are formed in the upper portion of the inner wall of the side, which is opposite to the air holes II, of the air holes, an air plate is inserted and sleeved in the side grooves, the air plate extends out of the side grooves at one end far away from the side grooves, the air plate is matched with the shape of the side grooves, the upper surface of the air plate, which extends out of the side grooves, is correspondingly fixedly connected with an insertion pipe, and the insertion pipe is opposite to the air holes I and is matched with the lower port of the air holes II.
Compared with the prior art, the invention has the beneficial effects that:
this full-automatic low temperature storage equipment of stem cell, when the staff presss from both sides the test tube that is equipped with the stem cell, need press the handle with the hand from both sides, simultaneously make the clamp press from both sides the upper end of getting the test tube that is equipped with the stem cell at the inside of inner shell, when the handle is pressed, the board drives the slide bar and moves in the undercut for a while, thereby make the air slab stretch out sealed gas pocket second from the undercut, place after accomplishing in putting in the shell when the test tube that is equipped with the stem cell gets into, loosen the handle, make air pocket second and gas pocket first intercommunication, make nitrogen gas have a part in the inside of taking the shell, so when follow-up taking the test tube that is equipped with the stem cell, take the test tube through pressing the handle, thereby continue sealed gas pocket second, make nitrogen gas in the inside of taking the device slowly cool down to the test tube, simultaneously the staff can not contact the temperature of nitrogen gas, make the test tube that is equipped with the stem cell can be taken out safely, and the temperature of test tube that is equipped with the stem cell can be comparatively slow rise, avoid the test tube temperature change of putting the stem cell and burst.
According to the full-automatic low-temperature storage equipment for stem cells, the test tube filled with the stem cells is clamped in the inner shell through the pressing handle, the test tube filled with the stem cells is vertically inserted into the placing shell, after the taking device, the bottom plate device, the cooling device, the placing device and the top plate device are contacted, the handle is loosened, the test tube filled with the stem cells enters the inside of the placing shell, the bottom of the test tube filled with the stem cells can be contacted with the bottom bowl, the test tube filled with the stem cells presses down the bottom bowl, the descending upper column drives the first counter-clockwise rotation gear to drive the second counter-clockwise rotation gear to drive the connecting ring to rotate clockwise, the connecting ring is connected with the connecting plate, the sealing plate and the sealing belt are enabled to rotate clockwise with the connecting ring as an axis, so that the sealing plate moves out of the side hole, at the moment, when the test tube filled with the stem cells needs to be taken out, the side hole is opened or closed according to whether the test tube filled with the stem cells is placed in, and the self-adaptive performance of the device is improved.
According to the full-automatic low-temperature storage equipment for the stem cells, the first air bag is used for contacting the bottom of the test tube filled with the stem cells when the test tube filled with the stem cells enters the placing shell, so that the test tube filled with the stem cells cannot directly contact with the plugging plate, the first air bag has buffering capacity due to the self-characteristics of the first air bag, the test tube filled with the stem cells is protected, and the safety of the test tube filled with the stem cells is improved.
According to the full-automatic low-temperature storage equipment for the stem cells, through the design of the left air bag and the right air bag, when the test tube filled with the stem cells is put in, the bottom of the test tube filled with the stem cells is stressed to the center by the first air bag, so that the test tube filled with the stem cells is positioned just before entering the placing shell, the test tube filled with the stem cells can be placed right against the bottom bowl, and the positioning capability of the device is improved.
This full-automatic low temperature storage equipment of stem cell is put into through the test tube that is equipped with the stem cell to open the side opening, fill nitrogen gas from the nitrogen valve in the inside of cooling shell, make the test tube that is equipped with the stem cell by low temperature storage, accomplish the back at bottom plate device and roof device butt joint, the inside of taking the device can also be entered into to the inside nitrogen gas of cooling shell, thereby make nitrogen gas only exist around the test tube that is equipped with the stem cell, when depositing the test tube that is equipped with the stem cell in the case like this, make nitrogen gas not need to be full of the inside of box through being equipped with this kind of device, the quantity of nitrogen gas that reduces, reduce the consumption to nitrogen gas.
This full-automatic low temperature storage equipment of stem cell, when taking the test tube that is equipped with the stem cell, when taking off the bottom bowl to the test tube that is equipped with the stem cell, because the existence of spring one for the bottom bowl can resume the normal position, gear one, gear two, go-between, shutoff board and shutoff area all can resume the normal position simultaneously, shutoff board and shutoff area can block the side opening immediately this moment, the handle that presses when taking simultaneously also can make board one drive slide bar and air slab close gas pocket two, thereby make when the test tube that is equipped with the stem cell is taken out, nitrogen gas has been isolated inside the device, the at utmost avoids the leak of nitrogen gas, practice thrift nitrogen gas.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is an external schematic view of a cooling device according to the present invention;
FIG. 3 is a schematic view of the interior of the cooling device of the present invention;
FIG. 4 is a schematic view of a side hole of the present invention;
FIG. 5 is a schematic view of the interior of the cooling device of the present invention;
FIG. 6 is an enlarged schematic view of the invention at A;
FIG. 7 is a schematic view of a pick-up device according to the present invention;
FIG. 8 is a schematic view of the interior of the movable cartridge of the present invention;
fig. 9 is a schematic view of a base plate of the present invention.
In the figure: 1. a cooling device; 101. cooling the shell; 102. a nitrogen valve; 2. a placement device; 201. placing a shell; 202. a bottom hole; 203. a side hole; 3. a communication device; 301. a bottom post; 302. loading a column; 303. a bottom bowl; 304. a first spring; 305. a first gear; 306. a first gear shaft; 307. a second gear; 308. a second gear shaft; 309. a connecting ring; 310. a connecting plate; 311. a plugging plate; 312. an air bag I; 313. an air bag II; 314. a plugging band; 4. a top plate device; 401. a top plate; 402. an air hole I; 403. a cross-shaped opening pad; 5. a pick-up device; 501. taking the shell; 502. an inner case; 503. a clamping groove; 504. a clip; 505. column one; 506. a second spring; 507. a movable cylinder; 508. column two; 509. a first plate; 510. a third spring; 511. a bottom groove; 512. a slide block; 513. a slide bar; 514. a handle; 6. a base plate device; 601. a bottom plate; 602. an air hole II; 603. a side groove; 604. an air plate; 605. and (3) inserting a tube.
Description of the embodiments
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-9, the present invention provides a technical solution: the utility model provides a full-automatic low temperature storage facilities of stem cell, including heat sink 1, heat sink 1 includes cooling shell 101, the inside center department of cooling shell 101 is provided with placer 2, placer 2 is including placing shell 201, the upper port of placing shell 201 flushes with the upper port of cooling shell 101, the lower extreme of placing shell 201 is in the top of the lower extreme of cooling shell 101, bottom hole 202 has been seted up in the middle part of placing shell 201, side wall all runs through about the middle part of placing shell 201 has been seted up side hole 203, the inside lower extreme of cooling shell 101 is provided with intercommunication device 3, intercommunication device 3 includes collet 301, the lower extreme fixed connection of collet 301 is the lower inner wall middle part of cooling shell 101, the upper end activity of collet 301 has cup jointed up post 302, the upper end of collet 302 passes bottom hole 202 and with collet 202 adaptation, the upper end of collet 302 stretches into the inside of placing shell 201, the upper end fixedly connected with collet 303, collet 303 presents the bowl form, collet 304 is fixedly connected with spring 304 in the lower lateral wall of collet 303, the lower extreme fixedly connected with collet 304 of collet 201, the outer side of collet 302.
The nitrogen valve 102 is fixedly arranged at the upper part of the front side of the cooling shell 101, and the nitrogen valve 102 is communicated with the inside of the cooling shell 101.
The outside of the upper column 302 presents an annular bending shape (as shown in fig. 6), the lower end of the upper column 302 is positioned below the placing shell 201, the left side and the right side of the upper column 302 are both meshed with a first gear 305, the middle part of the first gear 305 is connected with a first gear shaft 306 in a penetrating way, the first gear shaft 306 is connected with the first gear 305 in a rotating way through a bearing, the front end and the rear end of the first gear shaft 306 are fixedly connected with the inner side wall of the cooling shell 101, the first gear 305 is meshed with a second gear 307 on the side far away from the upper column 302, the middle part of the second gear 307 is connected with a second gear shaft 308 in a penetrating way, the second gear shaft 308 is connected with the second gear 307 in a rotating way through a bearing, and the front end and the rear end of the second gear shaft 308 are fixedly connected with the inner side wall of the cooling shell 101.
The front and rear surfaces of the gear II 307 are fixedly connected with connecting rings 309, the connecting rings 309 are sleeved on the outer side of the gear II 308, the upper side walls of the front and rear connecting rings 309 connected with the same gear II 307 are jointly connected with connecting plates 310, the connecting plates 310 are arc-shaped, the connecting plates 310 are fixedly connected with sealing strips 314 at the ends far away from the connecting rings 309, the sealing strips 311 are correspondingly inserted into the side holes 203 and are matched with the side holes 203, the sealing strips 311 are arc-shaped, the lower ends of the sealing strips 311 are contacted with the lower inner wall of the side holes 203, the upper ends of the sealing strips 311 penetrate through the side holes 203 and extend into the inner part of the placing shell 201, the upper ends of the sealing strips 311 are fixedly connected with an air bag I312, the lower ends of the sealing strips 311 are fixedly connected with an air bag II 313, the upper edges of the sealing strips 311 are fixedly connected with a sealing strip 314 when the lower ends of the sealing strips 311 are contacted with the lower inner wall of the side holes 203, the sealing strips 314 are integrally U-shaped, the sealing strips 314 are fixedly connected with the inner wall of the cooling shell 101 through the side holes 203, the sealing strips 311 are connected with the side holes 101, and the sealing strips 203 are connected with the sealing strips 203 at one ends of the sealing strips 102.
The upper ports of the cooling shell 101 and the nitrogen valve 102 are jointly provided with a top plate device 4, the top plate device 4 comprises a top plate 401, the top plate 401 is annular, the inner ring of the top plate 401 is fixedly connected with the outer side wall of the placing shell 201, the outer ring of the top plate 401 is fixedly connected with the inner side wall of the cooling shell 101, air holes I402 are formed in the left side and the right side of the upper surface of the top plate 401, and cross opening pads 403 are fixedly connected in the inner portion of the air holes I402 in an embedded mode.
The cooling shell 101 and the nitrogen valve 102 are provided with the take device 5 in the top, take device 5 is including taking the shell 501, take the shell 501 and present the upper hemisphere shell form, take the shell 501 and the diameter of cooling shell 101 the same, the inside center department of taking the shell 501 is provided with inner shell 502, inner shell 502 presents the semi-cylindrical shell form, the diameter of inner shell 502 and placing shell 201 is the same, take the lower port of shell 501 and inner shell 502 to flush, clamping grooves 503 have all been seted up about the inside wall of inner shell 502, two clamping grooves 503 symmetry, the inside embedding of clamping grooves 503 is connected with clamping clips 504, clamping clips 504 and clamping grooves 503 adaptation, the side middle part fixedly connected with post one 505 that two clamping clips 504 deviate from, the one end fixedly connected with clamping clip 504 and the other end fixedly connected with clamping groove 503 of spring two 506 have been cup jointed in the one end outside that is close to clamping clips 504 to post one 505.
The first column 505 penetrates through the side wall of the inner shell 502 at the end far away from the clamping 504 and is fixedly connected with a movable barrel 507, the movable barrel 507 is positioned between the taking shell 501 and the inner shell 502, the movable barrel 507 is in a horizontal cylindrical shell shape, the movable barrel 507 penetrates through and is sleeved with the second column 508 at the end far away from the first column 505, the second column 508 is inserted into the movable barrel 507 at the end near the movable barrel 507 and is fixedly connected with the first plate 509, the first plate 509 is fixedly connected with the third spring 510 at the end far away from the second column 508, and the third spring 510 is fixedly connected with the inner wall of the movable barrel 507 at the end far away from the first plate 509.
The end of the lower side wall of the movable barrel 507, which is close to the second column 508, is provided with a bottom groove 511, the lower side wall of the first plate 509 is fixedly connected with a sliding block 512, the sliding block 512 is inserted into the bottom groove 511, the sliding block 512 can only slide in the bottom groove 511, the lower end of the sliding block 512 is fixedly connected with a sliding rod 513, and the second column 508 penetrates through the side wall of the taking shell 501 at the end far away from the movable barrel 507 and is fixedly connected with a handle 514.
The lower ports of the taking shell 501 and the inner shell 502 are provided with a bottom plate device 6, the bottom plate device 6 comprises a bottom plate 601, the bottom plate 601 presents a ring shape, the inner ring of the bottom plate 601 is fixedly connected with the outer side wall of the lower port of the inner shell 502, the outer ring of the bottom plate 601 is fixedly connected with the inner side wall of the lower port of the taking shell 501, the left side and the right side of the surface of the bottom plate 601 are provided with air holes II 602, the air holes II 602 are opposite to the air holes I402, the upper portion of the inner wall of the side, away from the second air holes 602, of the side, is provided with a side groove 603, an air plate 604 is inserted and sleeved in the side groove 603, the air plate 604 extends out of the side groove 603 at the end, away from the side groove 603, of the air plate 604, the air plate 604 is in shape adaptation with the side groove 603, the upper surface, extending out of the side groove 603, of the air plate 604 is correspondingly and fixedly connected with a sliding rod 513, the lower port of the second air holes 602 is fixedly connected with a cannula 605, and the cannula 605 is opposite to and is in adaptation with the first air holes 402.
Working principle:
the first step: by pressing the handle 514 to clamp the stem cell-containing test tube inside the inner case 502, the stem cell-containing test tube is vertically inserted into the placing case 201, after the taking device 5, the bottom plate device 6, the cooling device 1, the placing device 2 and the top plate device 4 are contacted, the handle 514 is loosened, the stem cell-containing test tube enters the inside of the placing case 201, the bottom of the stem cell-containing test tube contacts the bottom bowl 303, the stem cell-containing test tube presses down the bottom bowl 303, the descending upper column 302 drives the gear one 305 to rotate anticlockwise, the gear two 307 is driven to rotate clockwise, the connecting ring 309 is driven to rotate clockwise, the sealing plate 311 is driven to rotate clockwise by taking the connecting ring 309 as an axis under the connection of the connecting plate 310, so that the sealing plate 311 is moved out of the side hole 203, at this time, the sealing plate 311 and the sealing belt 314 open the side hole 203, when the stem cell-containing test tube needs to be taken out, the moving directions of the communicating device 3 are opposite, at this time, the sealing plate 311 and the sealing belt 314 reseal the side hole 203, the device opens or closes the side hole 203 according to whether the stem cell-containing test tube is put in, and the self-adapting performance of the device is improved.
And a second step of: by the existence of the first air bag 312, when the test tube filled with the stem cells enters the inside of the placing shell 201, the first air bag 312 is contacted with the bottom of the test tube filled with the stem cells, so that the test tube filled with the stem cells cannot directly contact with the plugging plate 311, and the first air bag 312 has buffering capacity due to the self-characteristics of the first air bag 312, thereby helping to protect the test tube filled with the stem cells and improving the safety of the test tube filled with the stem cells.
And a third step of: through the design of the left and right air bags one 312, when the test tube filled with stem cells is put in, the bottom of the test tube filled with stem cells is stressed to the center by the two air bags one 312, so that the test tube filled with stem cells is positioned when the test tube filled with stem cells just enters the placing shell 201, the test tube filled with stem cells can be placed just opposite to the bottom bowl 303, and the positioning capability of the device is improved.
Fourth step: through the put-in of the test tube that is equipped with stem cells to open side opening 203, fill nitrogen gas from nitrogen valve 102 into the inside of cooling shell 101, make the test tube that is equipped with stem cells by the low temperature storage, after bottom plate device 6 and roof device 4 dock the completion, the inside nitrogen gas of cooling shell 101 also can enter into the inside of taking device 5, thereby make nitrogen gas only exist with the test tube that is equipped with stem cells around, when depositing the test tube that is equipped with stem cells in the case like this, make nitrogen gas not need to be full of inside the box through being equipped with this kind of device, the quantity of nitrogen gas that reduces the consumption to nitrogen gas.
Fifth step: when the bottom of the test tube filled with the stem cells is separated from the bottom bowl 303 during taking the test tube filled with the stem cells, the bottom bowl 303 can be restored to the original position due to the existence of the first spring 304, meanwhile, the first gear 305, the second gear 307, the connecting ring 309, the plugging plate 311 and the plugging belt 314 can be restored to the original position, at the moment, the plugging plate 311 and the plugging belt 314 can immediately plug the side holes 203, and meanwhile, the first plate 509 can be driven by the handle 514 pressed during taking to drive the sliding rod 513 and the air plate 604 to close the second air holes 602, so that nitrogen is isolated inside the device when the test tube filled with the stem cells is taken out, the leakage of the nitrogen is avoided to the greatest extent, and the nitrogen is saved.
Sixth step: when a staff clamps the test tube filled with stem cells, the handle 514 is required to be pressed from two sides by hand, meanwhile, the clamp 504 clamps the upper end of the test tube filled with stem cells in the inner shell 502, when the handle 514 is pressed, the first plate 509 drives the sliding rod 513 to move in the bottom groove 511, so that the air plate 604 stretches out of the sealing air hole II 602 from the side groove 603, after the test tube filled with stem cells enters the placing shell 201 to be placed, the handle 514 is loosened, so that the air hole II 602 is communicated with the air hole I402, a part of nitrogen is stored in the taking shell 501, so that the test tube is taken by pressing the handle 514 when the test tube filled with stem cells is taken later, the air hole II 602 is sealed continuously, so that the nitrogen is slowly cooled in the taking device 5, the staff can not be contacted with the temperature of the nitrogen, the test tube filled with stem cells can be taken out safely, the temperature of the test tube filled with stem cells can be slowly increased, and the temperature of the test tube filled with stem cells is prevented from being burst, and the test tube filled with stem cells is prevented from being changed rapidly.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (1)
1. The utility model provides a full-automatic low temperature storage equipment of stem cell, includes heat sink (1), heat sink (1) is including cooling shell (101), the inside center department of cooling shell (101) is provided with placer (2), placer (2) are including placing shell (201), the last port of placing shell (201) flushes with the last port of cooling shell (101), the lower extreme of placing shell (201) is in the top of the lower extreme of cooling shell (101), its characterized in that: the middle part of the lower end of the placing shell (201) is penetrated and provided with a bottom hole (202), the left and right side walls of the middle part of the placing shell (201) are penetrated and provided with side holes (203), the lower end of the inside of the cooling shell (101) is provided with a communication device (3), the communication device (3) comprises a bottom column (301), the lower end of the bottom column (301) is fixedly connected with the middle part of the lower inner wall of the cooling shell (101), the upper end of the bottom column (301) is movably sleeved with an upper column (302), the upper end of the upper column (302) penetrates through the bottom hole (202) and is matched with the bottom hole (202), the upper end of the upper column (302) stretches into the inside of the placing shell (201), the upper end of the upper column (302) is fixedly connected with a bottom bowl (303), the bottom bowl (303) is in a bowl shape, the lower side wall of the bottom bowl (303) is fixedly connected with a first spring (304), the lower end of the first spring (304) is fixedly connected with the lower inner wall of the placing shell (201), and the first spring (302) is fixedly connected with the outer side of the upper column (302).
A nitrogen valve (102) is fixedly arranged at the upper part of the front side of the cooling shell (101), and the nitrogen valve (102) is communicated with the inside of the cooling shell (101);
the cooling device is characterized in that top plate devices (4) are arranged at the upper ports of the cooling shell (101) and the nitrogen valve (102) together, the top plate devices (4) comprise top plates (401), the top plates (401) are annular, the inner rings of the top plates (401) are fixedly connected with the outer side walls of the placing shell (201), the outer rings of the top plates (401) are fixedly connected with the inner side walls of the cooling shell (101), air holes I (402) are formed in the left side and the right side of the upper surface of the top plates (401), and cross opening gaskets (403) are embedded in the air holes I (402);
the cooling device comprises a cooling shell (101) and a nitrogen valve (102), wherein a taking device (5) is arranged above the cooling shell (101) and the nitrogen valve (102), the taking device (5) comprises a taking shell (501), the taking shell (501) is in an upper hemispherical shell shape, the diameter of the taking shell (501) is the same as that of the cooling shell (101), an inner shell (502) is arranged at the center of the inside of the taking shell (501), the inner shell (502) is in an upper semi-cylindrical shell shape, the diameter of the inner shell (502) is the same as that of a placing shell (201), the taking shell (501) is flush with the lower port of the inner shell (502), clamping grooves (503) are formed in the left side and the right side of the inner side wall of the inner shell (502), the two clamping grooves (503) are symmetrical, clamping clips (504) are embedded and are connected with one another, a first column (505) is fixedly connected to the middle of one side of the two clamping clips (504), and one end (506) of the other end (506) of each clamping clip (504) is fixedly connected with one end of the other, which is in a sleeved connection with the two springs (506);
the first column (505) penetrates through the side wall of the inner shell (502) at the end far away from the clamping (504) and is fixedly connected with a movable barrel (507), the movable barrel (507) is positioned between the taking shell (501) and the inner shell (502), the movable barrel (507) is in a horizontal cylindrical shell shape, the movable barrel (507) penetrates through and is sleeved with a second column (508) at the end surface far away from the first column (505), the second column (508) is inserted into the movable barrel (507) at the end close to the movable barrel (507) and is fixedly connected with a first plate (509), the first plate (509) is fixedly connected with a third spring (510) at the end far away from the second plate (509), and the third spring (510) is fixedly connected with the inner wall of the movable barrel (507) at the end far away from the first plate (509);
the lower side wall of the movable barrel (507) is close to the end of the second column (508) and provided with a bottom groove (511), the lower side wall of the first plate (509) is fixedly connected with a sliding block (512), the sliding block (512) is inserted into the bottom groove (511), the lower end of the sliding block (512) is fixedly connected with a sliding rod (513), and the second column (508) penetrates through the side wall of the taking shell (501) at the end far away from the movable barrel (507) and is fixedly connected with a handle (514);
the lower port of the taking shell (501) and the lower port of the inner shell (502) are provided with a bottom plate device (6), the bottom plate device (6) comprises a bottom plate (601), the bottom plate (601) is annular, the inner ring of the bottom plate (601) is fixedly connected with the outer side wall of the lower port of the inner shell (502), the outer ring of the bottom plate (601) is fixedly connected with the inner side wall of the lower port of the taking shell (501), the left and right sides of the surface of the bottom plate (601) are respectively provided with an air hole II (602), the air holes II (602) are opposite to the air holes I (402), the upper parts of the inner walls of the sides of the two air holes II (602) which face away from each other are provided with side grooves (603), an air plate (604) is inserted and sleeved in the inner side of the side groove (603), the air plate (604) extends out of the side grooves (603) at the end far away from the side grooves (603), the air plate (604) is matched with the shape, the upper surface of the outer side of the side groove (603) is correspondingly fixedly connected with an air hole II (513), the upper surface of the air plate (605) extends out of the side groove (603), the upper surface of the side groove (603) is opposite to the air hole II is provided with an air hole II, and the lower port (605) is fixedly connected with the air hole II (402), and is opposite to the air hole II (602).
The outer side of the upper column (302) is in an annular bent shape, the lower end of the upper column (302) is arranged below the placing shell (201), gears I (305) are meshed with the left side and the right side of the upper column (302), a gear shaft I (306) is connected with the middle of the gears I (305) in a penetrating mode, the gear shaft I (306) is connected with the gears I (305) in a rotating mode through bearings, the front end and the rear end of the gear shaft I (306) are fixedly connected with the inner side walls of the cooling shell (101), the gear I (305) is meshed with a gear II (307) on the side far away from the upper column (302), a gear shaft II (308) is connected with the middle of the gear II (307) in a penetrating mode, and the gear II (308) is connected with the inner side walls of the cooling shell (101) in a rotating mode through bearings;
the front surface and the rear surface of the gear II (307) are fixedly connected with connecting rings (309), the connecting rings (309) are sleeved on the outer side of the gear II (308), the upper side walls of the front connecting ring (309) and the rear connecting ring (309) which are connected with the same gear II (307) are jointly connected with connecting plates (310), the connecting plates (310) are in arc shapes, the connecting plates (310) are fixedly connected with plugging plates (311) at the end far away from the connecting rings (309), the plugging plates (311) are correspondingly inserted into the side holes (203) and are matched with the side holes (203), the plugging plates (311) are in arc shapes, the lower ends of the plugging plates (311) are in contact with the lower inner walls of the side holes (203), the upper ends of the plugging plates (311) penetrate through the side holes (203) and extend into the inner part of the placing shell (201), the upper ends of the plugging plates (311) are fixedly connected with air bags (312), the lower ends of the connecting plates (310) are fixedly connected with air bags II (313), the upper edges of the plugging plates (311) are fixedly connected with the upper edges of the plugging plates (314) which are correspondingly inserted into the side holes (203) and are in the shape of the whole plugging plates (314) and are far away from the inner walls (101), the end of the plugging belt (314) connected with the cooling shell (101) is positioned above the end connected with the plugging plate (311), and the nitrogen valve (102) is positioned above the plugging belt (314).
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| CN202210304279.7A CN114514925B (en) | 2022-03-25 | 2022-03-25 | Full-automatic low temperature storage equipment of stem cell |
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| CN202210304279.7A CN114514925B (en) | 2022-03-25 | 2022-03-25 | Full-automatic low temperature storage equipment of stem cell |
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| CN114514925A (en) | 2022-05-20 |
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