CN212244214U

CN212244214U - A cryopreserved box for storing stem cell

Info

Publication number: CN212244214U
Application number: CN202020305161.2U
Authority: CN
Inventors: 陈碧清; 陈庆林
Original assignee: Beijing Hongxin Stem Cell Biotechnology Co ltd
Current assignee: Beijing Hongxin Stem Cell Biotechnology Co ltd
Priority date: 2020-03-12
Filing date: 2020-03-12
Publication date: 2020-12-29
Anticipated expiration: 2030-03-12

Abstract

The utility model discloses a cryopreservation box for storing stem cells, which comprises an upper box body and a lower box body, wherein the upper surface of the lower box body is inwards sunken to form a lower containing groove for placing a stem cell collection bag, the lower surface of the upper box body is inwards sunken to form an upper containing groove, a circle of liquid inlet holes are arranged on the side wall of the opening part of the upper containing groove in an annular distribution manner, after the upper box body and the lower box body are closed, the upper containing groove and the lower containing groove form an containing cavity, liquid nitrogen enters the containing cavity from the liquid inlet holes after the cryopreservation box is placed in a liquid nitrogen tank, a negative pressure suction device is arranged on the upper box body and is used for accelerating the liquid nitrogen to enter the containing cavity, the utility model can enable the liquid nitrogen to more rapidly fill the blood bag around on the premise of ensuring the sealing effect of the box body, the use effect is good, and a small part of the liquid nitrogen can, so as to keep the internal low-temperature state of the box body when waiting to be opened, and reduce the waiting time and cause potential influence on the recovery and survival rate of the cryopreserved cells.

Description

A cryopreserved box for storing stem cell

Technical Field

The utility model relates to a stem cell preserves technical field, specifically is a cryopreserved box for storing stem cell.

Background

The hematopoietic stem cell sample in the entity library is a mixture of hematopoietic stem cells, white blood cells and platelets from which most of blood plasma and red blood cells are removed, the prepared hematopoietic stem cells are transferred into a cryopreservation bag from an acquisition bag, and the cryopreservation bag is placed in an aluminum box and finally placed in liquid nitrogen for long-term cryopreservation.

The cryopreservation box used at present still has the following defects that firstly, a tight closed structure around the cryopreservation box is not favorable for liquid nitrogen to fill the blood bag quickly, secondly, the existing cryopreservation box is taken out, air is rapidly desublimated and adhered to a closed part under the influence of residual liquid nitrogen on the surface of the box at the moment, so that the brake pad-rivet structure is difficult to push, the cryopreservation box is opened to wait for a period of time before, cell resuscitation is required to be completed within one minute, the waiting time causes potential influence on the resuscitation rate of the cryopreserved cells, and therefore if the cryopreservation box can be kept in a low-temperature state when waiting, the resuscitation rate of the cryopreserved cells can be improved.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cryopreserved box for storing stem cell makes liquid nitrogen more rapid filling blood bag around, taking out cryopreserved box back simultaneously, can keep the box body inside low temperature state when waiting to open, reduces latency and causes potential influence to cryopreserved cell resuscitation life rate.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a cryopreserved box for storing stem cell, includes box body and lower box body, it is articulated that the hinge is passed through to the one end of going up box body and lower box body, and the other end passes through magnetic connector and connects after closing the lid, the upper surface of box body is to inside sunken storage tank down that forms down for place stem cell and gather the bag, the lower surface of going up the box body is to the sunken storage tank that forms of inside, is the annular distribution on the opening part lateral wall of last storage tank and runs through and seted up round feed liquor hole, and last box body closes the lid with lower box body after, go up the storage tank and enclose into the holding chamber with lower storage tank, places the cryopreserved box back liquid nitrogen from feed liquor hole department entering holding chamber, is provided with negative pressure suction device on last box body for liquid.

Preferably, the negative pressure suction device comprises a first pressing plate, a first connecting column, a first spring and a first piston, the first piston is connected to the inner wall of the upper accommodating groove in a sliding mode, the first connecting column is coaxially fixed to the upper surface of the first piston, the upper end of the first connecting column penetrates out of the top wall of the upper accommodating groove, the first connecting column is coaxially fixed to the lower surface of the first pressing plate, and the first spring is arranged between the upper surface of the upper box body and the lower surface of the first pressing plate and sleeved outside the first connecting column.

Preferably, a first hollow cavity is formed between the outer wall of the upper box body and the wall of the upper containing groove, a first one-way air valve is arranged on the outer side wall of the upper box body, the first one-way air valve exhausts air from the first hollow cavity in the flowing direction, and the first hollow cavity is vacuumized by connecting an air extractor outside the first one-way air valve to form a vacuum layer.

Preferably, be provided with the stock solution chamber down between the outer wall of the lateral wall of box body to the lower storage tank, be annular distribution and run through on the lateral wall of stock solution chamber and the equal height of lower storage tank and seted up round drainage hole, will freeze and deposit the box and place back liquid nitrogen in the liquid nitrogen cylinder and follow drainage hole department and get into the stock solution chamber, be provided with liquid nitrogen drainage device on the box body down for liquid nitrogen gets into the stock solution chamber with higher speed, and most liquid nitrogen of discharge stock solution intracavity when taking out the freezing box of depositing.

Preferably, liquid nitrogen drainage device includes second clamp plate, second spliced pole, second spring and second piston, second piston sliding connection is on the inner wall in liquid storage cavity, the diapire of storage tank is worn out down at the lower surface of second piston and its lower extreme to the coaxial fixing of second spliced pole, the coaxial upper surface of fixing at the second clamp plate of second spliced pole, the second spring sets up between the upper surface of the lower surface of box body and second clamp plate down to the cover is established in the outside of second spliced pole.

Preferably, the liquid storage cavity is internally provided with a partition plate, the partition plate is arranged outside the lower storage tank wall and coaxially arranged with the lower storage tank, the upper end of the partition plate is fixed on the top wall of the liquid storage cavity, the lower end of the partition plate is fixed on the outer tank wall of the lower storage tank, a second hollow cavity is formed by the partition plate, the top wall of the liquid storage cavity and the lower storage tank outer tank wall in a surrounding mode, a second one-way air valve communicated with the second hollow cavity and the outside is arranged on the lower storage tank wall, the circulation direction of the second one-way air valve is that the second hollow cavity exhausts outwards, and the second hollow cavity is vacuumized by an air pumping device connected to the outside of the second one-way.

Preferably, the number of the liquid inlet holes is 20.

Preferably, the number of the drainage holes is 6.

Compared with the prior art, the beneficial effects of the utility model are as follows:

the negative pressure suction device is arranged at the upper box body, so that after the cryopreservation box is placed in the liquid nitrogen tank, liquid nitrogen can more quickly enter the accommodating cavity from the liquid inlet hole and fill the periphery of the blood bag;

the utility model discloses a set up stock solution chamber and liquid nitrogen drainage device, when placing the freezing box in the liquid nitrogen container, liquid nitrogen drainage device can extract liquid nitrogen to the stock solution chamber, when taking out the freezing box, through pressing from both sides tight first clamp plate and second clamp plate, can discharge most liquid nitrogen in the box body, keep a little liquid nitrogen as the cold source simultaneously to keep the box body in the time of waiting to open the low temperature state of box body inside, can avoid causing the waste of liquid nitrogen again simultaneously;

thirdly, the utility model discloses a set up the vacuum layer in the inside of last box body and lower box body, can reduce the inside thermal conductivity for external world of box body after taking out the cryopreserved box, keep the inside low temperature state of box body in the time of waiting for opening of box body.

Drawings

FIG. 1 is a schematic view of the cover closing structure of the present invention;

FIG. 2 is a schematic view of the open structure of the present invention;

FIG. 3 is a cross-sectional view of the upper case;

FIG. 4 is a cross-sectional view of the lower case;

in the figure: 1-upper box body, 2-lower box body, 3-hinge, 4-liquid inlet hole, 5-drainage hole, 6-first pressing plate, 7-first connecting column, 8-first spring, 9-first piston, 10-upper containing groove, 11-lower containing groove, 12-first hollow cavity, 13-first one-way air valve, 14-second pressing plate, 15-second connecting column, 16-second spring, 17-second piston, 18-partition plate, 19-liquid storage cavity, 20-magnetic connecting piece, 21-second one-way air valve and 22-second hollow cavity.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 4, the present invention provides a technical solution: the utility model provides a cryopreserved box for storing stem cell, includes box body 1 and lower box body 2, it is articulated through hinge 3 to go up the one end of box body 1 and lower box body 2, and the other end passes through magnetic connecting piece 20 to connect after closing the lid to conveniently open the box body, all parts that should cryopreserved box simultaneously all use the same material preparation of liquid nitrogen container.

Storage tank 11 under the upper surface of box body 2 is sunken to inside and forms down for place stem cell and gather the bag, storage tank 10 is formed to sunken inside at the lower surface of last box body 1, it runs through and has seted up 20 feed liquor holes 4 altogether to be the annular distribution on the opening part lateral wall of last storage tank 10, after last box body 1 closes the lid with lower box body 2, go up storage tank 10 and lower storage tank 11 and enclose into the holding chamber, will freeze the box and place back liquid nitrogen in the liquid nitrogen container and get into the holding chamber from feed liquor hole 4, be provided with negative pressure suction device on last box body 1, liquid nitrogen entering holding chamber can accelerate.

Specifically, the negative pressure suction device comprises a first pressing plate 6, a first connecting column 7, a first spring 8 and a first piston 9, wherein the first piston 9 is slidably connected to the inner wall of an upper accommodating groove 10, the first connecting column 7 is coaxially fixed on the upper surface of the first piston 9, the upper end of the first connecting column penetrates out of the top wall of the upper accommodating groove 10, the first connecting column 7 is coaxially fixed on the lower surface of the first pressing plate 6, the first spring 8 is arranged between the upper surface of the upper box body 1 and the lower surface of the first pressing plate 6 and is sleeved outside the first connecting column 7, the first piston 9 can be pushed to move downwards by pressing the first pressing plate 6, after the first pressing plate 6 is loosened, the first spring 8 pushes the first pressing plate 6 outwards so as to drive the first piston 9 to move together, and when the first piston 9 moves upwards, negative pressure is formed in an accommodating cavity at the lower part of the first piston 9, thereby accelerating the flow speed of the liquid nitrogen passing through the liquid inlet hole 4 and ensuring that the liquid nitrogen quickly fills the periphery of the blood bag.

A liquid storage cavity 19 is arranged between the outer side wall of the lower box body 2 and the outer wall of the lower containing groove 11 and is used for storing a little liquid nitrogen as a cold source.

Particularly, be annular distribution and run through and seted up round 6 drainage holes 5 altogether on stock solution chamber 19 and the equal high lateral wall of lower storage tank 11, will freeze the box and place back liquid nitrogen in the liquid nitrogen container from drainage hole 5 entering stock solution chamber 19, be provided with liquid nitrogen drainage device on box body 2 down for liquid nitrogen gets into stock solution chamber 19 with higher speed, and most liquid nitrogen in discharge stock solution chamber 19 when taking out the box that freezes, remain few part liquid nitrogen, avoid causing the liquid nitrogen extravagant.

The liquid nitrogen drainage device comprises a second pressing plate 14, a second connecting column 15, a second spring 16 and a second piston 17, wherein the second piston 17 is connected to the inner wall of a liquid storage cavity 19 in a sliding mode, the second connecting column 15 is coaxially fixed to the lower surface of the second piston 17, the lower end of the second connecting column 15 penetrates out of the bottom wall of the lower containing groove 11 downwards, the second connecting column 15 is coaxially fixed to the upper surface of the second pressing plate 14, the second spring 16 is arranged between the lower surface of the lower box body 2 and the upper surface of the second pressing plate 14 and sleeved outside the second connecting column 15, when the cryopreservation box is placed in a liquid nitrogen tank, the liquid nitrogen drainage device has the same function as a negative pressure suction device, liquid nitrogen can enter the liquid storage cavity 19 in an accelerated mode, when the cryopreservation box is taken out, most of the liquid nitrogen in the liquid storage cavity 19 can be upwards discharged from the drainage hole 5 by pressing the second pressing plate 14, only a little liquid nitrogen is reserved in the annular space outside the partition plate 18, after the second pressing plate 14 is loosened, the reserved little liquid nitrogen flows to the lower part of the lower containing groove 11 and continues to be used as a cold source to keep the low-temperature state inside the box body in the waiting time for opening of the box body, and only 6 drainage holes 5 are arranged to reduce the phenomenon that the reserved little liquid nitrogen flows out too much to cause too little cold source.

After the cryopreservation box is taken out, the box body is opened for a period of time, in order to ensure the recovery rate of the cryopreserved stem cells, the thermal conductivity inside and outside the box body can be reduced, specifically, a first hollow cavity 12 is arranged between the outer wall of the upper box body 1 and the wall of the upper containing groove 10, a first one-way air valve 13 is arranged on the outer side wall of the upper box body 1, the flow direction of the first one-way air valve 13 is exhausting from the first hollow cavity 12 outwards, the first hollow cavity 12 is vacuumized by connecting an air extractor outside the first one-way air valve 13 to form a vacuum layer, a partition plate 18 is arranged in the liquid storage cavity 19, the partition plate 18 is arranged outside the wall of the lower containing groove 11 and is coaxial with the lower containing groove 11, the upper end of the partition plate 18 is fixed on the top wall of the liquid storage cavity 19, the lower end of the partition plate is fixed on the, And the outer wall of the lower containing groove 11 is surrounded to form a second hollow cavity 22, a second one-way air valve 21 communicated with the second hollow cavity 22 and the outside is arranged on the wall of the lower containing groove 11, the circulation direction of the second one-way air valve 21 is to exhaust from the second hollow cavity 22, the second hollow cavity 22 is vacuumized by connecting an air extractor outside the second one-way air valve 21, a vacuum layer is formed, the thermal conductivity from the inside of the box body to the outside can be effectively reduced by arranging the vacuum layer in the upper box body 1 and the lower box body 2 respectively, the low-temperature state in the box body is prolonged, and the recovery rate of the cryopreserved dry cells is improved.

The using method of the freezing storage box comprises the following steps: placing a blood bag filled with stem cells in a lower accommodating groove 11 of a lower box body 2, then covering an upper box body 1, simultaneously clamping a first pressing plate 6 and a second pressing plate 14 through a long clamp, placing the box body in a corresponding accommodating groove in a liquid nitrogen storage tank after clamping, then loosening the long clamp, and covering a cover of the liquid nitrogen storage tank;

when the cold source box is taken out, the cover of the liquid nitrogen liquid storage tank is opened, the first pressing plate 6 and the second pressing plate 14 are clamped simultaneously through the long clamp, most of liquid nitrogen in the box body is discharged through clamping, only a small amount of liquid nitrogen is reserved inside the box body to be used as a cold source, and after the cold source box is taken out, the second pressing plate 14 is placed downwards to wait for opening.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a cryopreserved box for storing stem cell, includes box body and lower box body, it is articulated through the hinge to go up the one end of box body and lower box body, and the other end passes through the magnetism connecting piece to be connected its characterized in that after closing the lid: the upper surface of box body is sunken to form storage tank down to inside for place stem cell and gather the bag, the lower surface of going up the box body is sunken to inside and forms storage tank, is the annular distribution on the opening part lateral wall of last storage tank and runs through and set up round feed liquor hole, and at last box body and lower box body close the lid after, go up the storage tank and enclose into the holding chamber with lower storage tank, is provided with negative pressure suction device on last box body for liquid nitrogen gets into the holding chamber with higher speed.

2. A cryopreservation cartridge for storing stem cells as claimed in claim 1 wherein: the negative pressure suction device comprises a first pressing plate, a first connecting column, a first spring and a first piston, wherein the first piston is connected to the inner wall of the upper accommodating groove in a sliding mode, the first connecting column is coaxially fixed to the upper surface of the first piston, the upper end of the first connecting column upwards penetrates out of the top wall of the upper accommodating groove, the first connecting column is coaxially fixed to the lower surface of the first pressing plate, and the first spring is arranged between the upper surface of the upper box body and the lower surface of the first pressing plate and sleeved outside the first connecting column.

3. A cryopreservation cartridge for storing stem cells as claimed in claim 1 wherein: the vacuum box is characterized in that a first hollow cavity is formed between the outer wall of the upper box body and the wall of the groove, a first one-way air valve is arranged on the outer side wall of the upper box body, the first one-way air valve exhausts air from the first hollow cavity in the flowing direction, and the first hollow cavity is vacuumized by connecting an air exhaust device outside the first one-way air valve to form a vacuum layer.

4. A cryopreservation cartridge for storing stem cells as claimed in claim 1 wherein: the lateral wall of box body is provided with the stock solution chamber down between to the outer wall of storage tank, be annular distribution on the lateral wall of the equal height of storage tank down and run through and seted up round drainage hole in the stock solution chamber, be provided with liquid nitrogen drainage device on the box body down for liquid nitrogen gets into the stock solution chamber with higher speed, and most liquid nitrogen of discharge stock solution intracavity when taking out the cryopreserved box.

5. A cryopreservation cartridge for storing stem cells as claimed in claim 4, wherein: the liquid nitrogen drainage device comprises a second pressing plate, a second connecting column, a second spring and a second piston, wherein the second piston is connected to the inner wall of the liquid storage cavity in a sliding mode, the second connecting column is coaxially fixed to the lower surface of the second piston and the bottom wall of the lower accommodating groove is downwards penetrated through by the lower end of the second piston, the second connecting column is coaxially fixed to the upper surface of the second pressing plate, the second spring is arranged between the lower surface of the lower box body and the upper surface of the second pressing plate, and the second spring is sleeved outside the second connecting column.

6. A cryopreservation cartridge for storing stem cells as claimed in claim 4, wherein: the utility model discloses a vacuum device, including storage tank, baffle, first one-way pneumatic valve, second one-way pneumatic valve, the liquid storage intracavity is provided with the baffle, the baffle sets up storage tank cell wall outside and sets up with lower storage tank coaxial line down, and the upper end of baffle is fixed on the roof in liquid storage chamber, and the lower extreme is fixed on the outer cell wall of storage tank down, through baffle, the roof in liquid storage chamber and down the outer cell wall of storage tank enclose become cavity in the second, be provided with the second one-way pneumatic valve of intercommunication cavity in the second and outside down on the storage tank cell wall, the circulation direction of second one-way pneumatic valve is for exhausting outside from cavity in the second, through cavity.

7. A cryopreservation cartridge for storing stem cells as claimed in claim 1 wherein: the number of the liquid inlet holes is 20.

8. A cryopreservation cartridge for storing stem cells as claimed in claim 4, wherein: the number of the drainage holes is 6.