CN114514925A - Full-automatic low temperature storage facilities of stem cell - Google Patents
Full-automatic low temperature storage facilities of stem cell Download PDFInfo
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- CN114514925A CN114514925A CN202210304279.7A CN202210304279A CN114514925A CN 114514925 A CN114514925 A CN 114514925A CN 202210304279 A CN202210304279 A CN 202210304279A CN 114514925 A CN114514925 A CN 114514925A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
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Abstract
The invention discloses a full-automatic low-temperature stem cell storage device, and relates to the technical field of low-temperature storage. This full-automatic low temperature storage facilities of stem cell, including the heat sink, the heat sink is including the cooling shell, the inside center department of cooling shell is provided with placer, placer is including placing the shell, the last port of placing the shell flushes with the last port of cooling shell, the lower extreme of placing the shell is in the top of the lower extreme of cooling shell, the lower extreme middle part of placing the shell is run through and has been seted up the bottom hole, the lateral wall all runs through and has been seted up the side opening about placing the middle part of shell, the inside lower extreme of cooling shell is provided with the intercommunication device, the intercommunication device includes the sill post, the lower inner wall middle part of the lower extreme fixed connection cooling shell of sill post, the upper end activity of sill post has been cup jointed the upper prop, the upper end of upper prop is passed the bottom hole and with the bottom hole adaptation, this kind of full-automatic low temperature storage facilities of stem cell, the staff can not contact the temperature of nitrogen gas yet, make the test tube that is equipped with the stem cell can be taken out by safety.
Description
Technical Field
The invention relates to the technical field of low-temperature storage, in particular to a full-automatic low-temperature storage device for stem cells.
Background
Generally, between stem cells and their terminally differentiated progeny, there is an intermediate population of progenitor cells, called "committed progenitors," which have limited expansion capacity and limited differentiation potential. These cell populations function to increase the number of differentiated cells produced after each division of the stem cells. Stem cells have the ability to self-renew, but stem cell division is in fact relatively asymmetric. Functionally, stem cells are cells with multipotentiality and self-renewal capacity, are the most primitive cells at the top of the cell line origin, and are capable of differentiating in vivo to give rise to cells of a particular tissue type. The stem cells lose their ability to self-renew once they differentiate into progenitor cells, undergo symmetric mitosis, and the number of progenitor cells is only supplemented by the proliferative differentiation of stem cells, but the progenitor cells still maintain a high proliferative capacity, and in the case of hematopoietic stem and progenitor cells, the massive expansion of cells during the course of individual lines of hematopoiesis depends mainly on the proliferation of hematopoietic progenitor cells. In adult organs, stem cells can repair tissue by constantly dividing, or are in a quiescent state as in mammalian brain tissue. Stem cells are capable of expanding their numbers by dividing symmetrically during their development, or self-renewing and producing more progenitor cells of different differentiation types by dividing asymmetrically.
After the stem cells are separated and cultured from different human tissues, the stem cells are detected and identified, and then the stem cells are frozen at low temperature, so that the stem cells can be revived for reinfusion to a patient when clinically needed, and the purpose of treating diseases is achieved. Therefore, it is necessary to preserve stem cells using a low-temperature storage device. Most stem cells need the low temperature to be preserved on the market at present, generally will have the test tube of stem cells to put into the case that is full of nitrogen gas and unify the saving, and at every turn when taking out the test tube from the incasement, nitrogen gas can spill over from the case mouth in a large number, make other test tubes that have stem cells in the incasement receive the influence, the nitrogen gas that overflows from the case mouth simultaneously still can the frostbite staff, very dangerous, consequently, need a full-automatic low temperature storage facilities of stem cells, be used for solving above-mentioned problem.
Disclosure of Invention
The present invention aims to provide a stem cell full-automatic low-temperature storage device to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a full-automatic low temperature storage equipment of stem cell, includes the heat sink, the heat sink includes the cooling shell, the inside center department of cooling shell is provided with placer, placer includes the placing shell, the last port of placing shell flushes with the last port of cooling shell, the lower extreme of placing shell is in the top of the lower extreme of cooling shell, the lower extreme middle part of placing shell is run through and is seted up the bottom hole, the lateral wall is run through and is seted up the side opening about the middle part of placing shell, the inside lower extreme of cooling shell is provided with the intercommunication device, the intercommunication device includes the foundation, the lower extreme fixed connection of foundation lower wall middle part of cooling shell, the activity of upper end of foundation has cup jointed the upper prop, the upper end of upper prop passes the bottom hole and with the bottom hole adaptation, the upper end of upper prop stretches into the inside of placing shell, the upper end fixedly connected with end bowl of upper prop, the bottom bowl is in a bowl shape, a first spring is fixedly connected to the lower side wall of the bottom bowl, the lower end of the first spring is fixedly connected with the lower inner wall of the placing shell, and the first spring is wound on the outer side of the upper end of the upper column.
Preferably, a nitrogen valve is fixedly mounted on the upper portion of the front side of the cooling shell and communicated with the inside of the cooling shell.
Preferably, the outside of going up the post presents annular form of buckling, the lower extreme of going up the post is in the below of placing the shell, the left and right sides of going up the post all meshes gear one, the middle part through connection of gear one has gear shaft one, gear shaft one is connected through the bearing rotation with gear one, the inside wall of the preceding back both ends fixed connection cooling shell of gear shaft one, gear one is meshing that the party of keeping away from the post has gear two, the middle part through connection of gear second has gear shaft two, gear shaft two passes through the bearing rotation with gear two and is connected, the inside wall of the preceding back both ends fixed connection cooling shell of gear shaft two.
Preferably, the equal fixedly connected with go-between in surface around gear two, the go-between cup joints in the outside of gear shaft two, two around being connected with same gear two the last lateral wall of go-between is connected with the connecting plate jointly, the connecting plate presents the arc plate-type, the connecting plate is in that one end fixedly connected with shutoff board of keeping away from the go-between, the shutoff board correspondence insert in the side opening and with the side opening adaptation, the shutoff board presents the arc plate-type, the lower extreme of shutoff board and the lower inner wall contact of side opening, the upper end of shutoff board is passed the side opening and is stretched into the inside of placing the shell, the upper end fixedly connected with gasbag one of shutoff board, the lower extreme fixedly connected with gasbag two of shutoff board, the upper portion edge fixedly connected with shutoff area of shutoff board, the shutoff area has elasticity, the shutoff area wholly presents the U style of calligraphy, the shutoff area is passed side opening fixedly connected on the inner wall of cooling shell in that the one end of keeping away from the shutoff board, the end of the plugging belt connected with the cooling shell is positioned above the end connected with the plugging plate, and the nitrogen valve is positioned above the plugging belt.
Preferably, the upper port of cooling shell and nitrogen gas valve is provided with the roof device jointly, the roof device includes the roof, the roof presents the annular, the lateral wall of shell is placed to the inner circle fixed connection of roof, the inside wall of the outer lane fixed connection cooling shell of roof, gas pocket one has all been seted up to the upper surface left and right sides of roof, the inside embedding fixedly connected with cross opening pad of gas pocket one.
Preferably, the top of cooling shell and nitrogen gas valve is provided with the device of taking, the device of taking is including the shell of taking, the shell of taking presents the episphere shell form, the shell of taking is the same with the diameter of cooling shell, the inside center department of the shell of taking is provided with the inner shell, the inner shell presents the semicylinder shell form, the inner shell is the same with the diameter of placing the shell, the lower port of shell and inner shell of taking flushes, the double-layered groove has all been seted up about the inside wall of inner shell, two the double-layered groove symmetry of pressing from both sides, the inside embedding in double-layered groove is connected with the clamping, clamping and double-layered groove adaptation, two that the clamping deviates from mutually is one side middle part fixedly connected with post one, spring two has been cup jointed in that one end outside that is close to the clamping, the one end fixed connection clamping and the other end fixed connection of spring two press from both sides the groove.
Preferably, the end, far away from the clamping, of the first column penetrates through the side wall of the inner shell and is fixedly connected with a movable cylinder, the movable cylinder is located between the taking shell and the inner shell, the movable cylinder is in a transverse cylindrical shell shape, a second column penetrates through and is sleeved on the end face, far away from the first column, of the movable cylinder, the end, close to the movable cylinder, of the second column is inserted into the inner portion of the movable cylinder and is fixedly connected with a first plate, the end, far away from the second column, of the first plate is fixedly connected with a third spring, and the end, far away from the first plate, of the third spring is fixedly connected with the inner wall of the movable cylinder.
Preferably, a bottom groove is formed in one end, close to the second column, of the lower side wall of the movable cylinder, a sliding block is fixedly connected to the lower side wall of the first plate, the sliding block is inserted into the bottom groove, a sliding rod is fixedly connected to the lower end of the sliding block, and the second column penetrates through the side wall of the taking shell and is fixedly connected with a handle at one end, far away from the movable cylinder.
Preferably, the lower port of the shell and the inner shell of taking is provided with a bottom plate device, the bottom plate device comprises a bottom plate, the bottom plate is annular, the outer side wall of the lower port of the inner shell is fixedly connected with the inner ring of the bottom plate, the inner side wall of the lower port of the shell of taking is fixedly connected with the outer ring of the bottom plate, the left side and the right side of the surface of the bottom plate are respectively provided with a second air hole, the second air hole is right opposite to the first air hole, a side groove is formed in the upper portion of the inner wall of one side, away from the second air hole, of the side groove, an air plate is inserted and sleeved into the inner side of the side groove, the end, away from the side groove, of the air plate stretches out of the side groove, the air plate is adaptive to the side groove, the upper surface, stretching out of the side groove, of the air plate corresponds to the fixedly connected slide bar, the lower port of the second air hole is fixedly connected with an insertion tube, and the insertion tube is right adaptive to the first air hole.
Compared with the prior art, the invention has the beneficial effects that:
(1) this full-automatic cryogenic storage equipment of stem cell, when the staff presss from both sides the test tube of being equipped with the stem cell, need press the handle with the hand from both sides, make the card press from both sides the upper end of getting the test tube of being equipped with the stem cell in the inside of inner shell simultaneously, when the handle is pressed, the board drives the slide bar for a while and moves in the kerve, thereby make the gas board stretch out sealed gas pocket two from the side channel, after the test tube that is equipped with the stem cell gets into to place in placing the shell and accomplish, loosen the handle, make gas pocket two and gas pocket one communicate, make nitrogen gas deposit partly in the inside of taking the shell, like this in the time of the test tube of follow-up taking being equipped with the stem cell, take the test tube through pressing the handle, thereby continue sealed gas pocket two, make nitrogen gas slow cooling to the test tube in the inside of taking the device, the staff can not contact with the temperature of nitrogen gas simultaneously, make the test tube that is equipped with stem cell can be taken out by safety, and the temperature of the test tube that is equipped with stem cell can comparatively slow rising, avoid the test tube temperature of being equipped with stem cell to change sharply and lead to the test tube to explode and split.
(2) The test tube filled with the stem cells is clamped in the inner shell by pressing the handle, the test tube filled with the stem cells is vertically inserted into the placing shell, after the taking device, the bottom plate device, the cooling device, the placing device and the top plate device are contacted, the handle is loosened, the test tube filled with the stem cells enters the placing shell, the bottom of the test tube filled with the stem cells can be contacted with the bottom bowl, the test tube filled with the stem cells presses down the bottom bowl, the descending upper column drives the gear to rotate anticlockwise and drives the gear to rotate clockwise, the connecting ring is driven to rotate clockwise, the plugging plate is driven to rotate clockwise by taking the connecting ring as an axis under the connection of the connecting plate, so that the plugging plate is moved out of the side hole, the side hole is opened by the plugging plate and the plugging belt, when the test tube filled with the stem cells needs to be taken out, the movement direction of the communicating device is opposite, at the moment, the side hole is sealed again by the plugging plate and the plugging belt, and the side hole is opened or closed by the device according to whether the test tube filled with stem cells is put in, so that the self-adaptive performance of the device is improved.
(3) This full-automatic low temperature storage facilities of stem cell, through the existence of gasbag one, when the test tube that is equipped with stem cell enters into and places the shell inside, with the test tube bottom contact that is equipped with stem cell be gasbag one to make the test tube that is equipped with stem cell can not directly contact with the shutoff board, and the self characteristic of gasbag one makes gasbag one have the ability of buffering, thereby help the protection test tube that is equipped with stem cell, improve the security of the test tube that is equipped with stem cell.
(4) This full-automatic low temperature storage facilities of stem cell, through the design of controlling two gasbag one, when the test tube that is equipped with stem cell was put into, two gasbag one make the test tube bottom atress that is equipped with stem cell to center department to make just entering into when placing the shell inside for the test tube that is equipped with stem cell advance line location in the test tube that is equipped with stem cell, make the test tube that is equipped with stem cell can just place the end bowl, improved the location ability of device.
(5) This kind of full-automatic low temperature storage facilities of stem cell, through the putting into of the test tube that is equipped with the stem cell, thereby open the side opening, fill nitrogen gas into the inside of cooling shell from the nitrogen gas valve, make the test tube that is equipped with the stem cell by low temperature storage, after bottom plate device and roof device butt joint are accomplished, the inside nitrogen gas of cooling shell also can enter into the inside of taking the device, thereby make nitrogen gas only exist with the test tube that is equipped with the stem cell around, like this deposit the test tube that is equipped with the stem cell in the case, make nitrogen gas need not be full of the box completely inside through being equipped with this kind of device, the quantity of nitrogen gas that reduces, reduce the consumption to nitrogen gas.
(6) This full-automatic low temperature storage facilities of stem cell, when taking to the test tube that is equipped with the stem cell, when the bottom of the test tube that is equipped with the stem cell breaks away from end bowl, because the existence of spring one, make end bowl can resume the normal position, gear one simultaneously, gear two, the go-between, shutoff board and shutoff area all can resume the normal position, shutoff board and shutoff area can block up the side opening immediately this moment, the handle that presses down when taking simultaneously also can make board one drive slide bar and gas board and close gas pocket two, thereby make when the test tube that is equipped with the stem cell is taken out, nitrogen gas has been completely cut off inside the device, the leakage of nitrogen gas is avoided to the at utmost, practice thrift nitrogen gas.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the exterior of the cooling device of the present invention;
FIG. 3 is a schematic view of the interior of the cooling device of the present invention;
FIG. 4 is a side hole schematic of the present invention;
FIG. 5 is a schematic plan view of the interior of the cooling device of the present invention;
FIG. 6 is an enlarged view of the invention at A;
FIG. 7 is a schematic view of a pickup apparatus according to the present invention;
FIG. 8 is a schematic view of the interior of the cartridge of the present invention;
FIG. 9 is a schematic view of a base plate according to the present invention.
In the figure: 1. a cooling device; 101. cooling the shell; 102. a nitrogen gas valve; 2. a placement device; 201. placing the shell; 202. a bottom hole; 203. a side hole; 3. a communication device; 301. a bottom pillar; 302. putting the column on; 303. a bottom bowl; 304. a first spring; 305. a first gear; 306. a first gear shaft; 307. a second gear; 308. a second gear shaft; 309. a connecting ring; 310. a connecting plate; 311. a plugging plate; 312. a first air bag; 313. a second air bag; 314. a sealing band; 4. a top plate arrangement; 401. a top plate; 402. a first air hole; 403. a cross-shaped open pad; 5. a taking device; 501. taking the shell; 502. an inner shell; 503. a clamping groove; 504. clamping; 505. a first column; 506. a second spring; 507. a movable barrel; 508. a second column; 509. a first plate; 510. a third spring; 511. A bottom groove; 512. a slider; 513. a slide bar; 514. a handle; 6. a backplane means; 601. a base plate; 602. A second air hole; 603. a side groove; 604. an air plate; 605. and (4) inserting a tube.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-9, the present invention provides a technical solution: a stem cell full-automatic low-temperature storage device comprises a cooling device 1, the cooling device 1 comprises a cooling shell 101, a placing device 2 is arranged at the center of the interior of the cooling shell 101, the placing device 2 comprises a placing shell 201, the upper port of the placing shell 201 is flush with the upper port of the cooling shell 101, the lower end of the placing shell 201 is positioned above the lower end of the cooling shell 101, a bottom hole 202 is formed in the middle of the lower end of the placing shell 201 in a penetrating manner, side holes 203 are formed in the left side wall and the right side wall of the middle of the placing shell 201 in a penetrating manner, a communication device 3 is arranged at the lower end of the interior of the cooling shell 101, the communication device 3 comprises a bottom column 301, the lower end of the bottom column 301 is fixedly connected with the middle of the lower inner wall of the cooling shell 101, an upper column 302 is movably sleeved at the upper end of the bottom column 301, the upper end of the upper column 302 penetrates through the bottom hole 202 and is matched with the bottom hole 202, the upper end of the upper column 302 extends into the interior of the placing shell 201, a bottom bowl 303 is fixedly connected with the upper end of the upper column 302, the bottom bowl 303 is in a bowl shape, a first spring 304 is fixedly connected to the lower side wall of the bottom bowl 303, the lower end of the first spring 304 is fixedly connected with the lower inner wall of the placing shell 201, and the first spring 304 is wound and connected to the outer side of the upper end of the upper column 302.
The upper part of the front side of the cooling shell 101 is fixedly provided with a nitrogen valve 102, and the nitrogen valve 102 is communicated with the inside of the cooling shell 101.
The outer side of the upper column 302 is in an annular bent shape (as shown in fig. 6), the lower end of the upper column 302 is located below the placing shell 201, the left side and the right side of the upper column 302 are both meshed with a first gear 305, the middle part of the first gear 305 is connected with a first gear shaft 306 in a penetrating manner, the first gear shaft 306 is connected with the first gear 305 through a bearing in a rotating manner, the front end and the rear end of the first gear shaft 306 are fixedly connected with the inner side wall of the cooling shell 101, the first gear 305 is meshed with a second gear 307 on the side far away from the upper column 302, the middle part of the second gear 307 is connected with a second gear shaft 308 in a penetrating manner, the second gear shaft 308 is connected with the second gear 307 through a bearing in a rotating manner, and the front end and the rear end of the second gear shaft 308 are fixedly connected with the inner side wall of the cooling shell 101.
The front surface and the rear surface of the second gear 307 are fixedly connected with connecting rings 309, the connecting rings 309 are sleeved on the outer sides of the second gear shafts 308, the upper side walls of the front connecting ring 309 and the rear connecting ring 309 connected with the same second gear 307 are jointly connected with connecting plates 310, the connecting plates 310 are arc-shaped, one ends of the connecting plates 310 far away from the connecting rings 309 are fixedly connected with plugging plates 311, the plugging plates 311 are correspondingly inserted into the side holes 203 and are matched with the side holes 203, the plugging plates 311 are arc-shaped, the lower ends of the plugging plates 311 are in contact with the lower inner walls of the side holes 203, the upper ends of the plugging plates 311 penetrate through the side holes 203 and extend into the placing shell 201, the upper ends of the plugging plates 311 are fixedly connected with air bag I312, the lower ends of the plugging plates 311 are fixedly connected with air bag II 313, the air bag II 313 play a role in buffering when the lower ends of the plugging plates 311 are in contact with the lower inner walls of the side holes 203, and the upper edges of the plugging plates 311 are fixedly connected with plugging belts 314, the blocking belt 314 has elasticity, the blocking belt 314 is U-shaped as a whole, the end of the blocking belt 314 far away from the blocking plate 311 penetrates through the side hole 203 and is fixedly connected to the inner wall of the cooling shell 101, the end of the blocking belt 314 connected with the cooling shell 101 is located above the end connected with the blocking plate 311, when the blocking plate 311 is located inside the side hole 203, the blocking belt 314 and the blocking plate 311 jointly seal the side hole 203, and the nitrogen gas valve 102 is located above the blocking belt 314.
The upper ports of the cooling shell 101 and the nitrogen valve 102 are jointly provided with a top plate device 4, the top plate device 4 comprises a top plate 401, the top plate 401 is annular, the outer side wall of the shell 201 is placed by the inner ring fixed connection of the top plate 401, the inner side wall of the outer ring fixed connection cooling shell 101 of the top plate 401, the left side and the right side of the upper surface of the top plate 401 are respectively provided with a first air hole 402, and the inside of the first air hole 402 is embedded into a cross-shaped opening pad 403.
The top of cooling shell 101 and nitrogen gas valve 102 is provided with device 5 of taking, device 5 of taking is including the shell 501 of taking, the shell 501 of taking presents upper hemisphere shell-like, the shell 501 of taking is the same with the diameter of cooling shell 101, the inside center department of the shell 501 of taking is provided with inner shell 502, inner shell 502 presents half cylinder shell-like, inner shell 502 is the same with the diameter of placing shell 201, the lower port of the shell 501 of taking and inner shell 502 flushes, double-layered groove 503 has all been seted up about the inside wall of inner shell 502, two double-layered groove 503 symmetries, the inside embedding of double-layered groove 503 is connected with clip 504, clip 504 and double-layered groove 503 adaptation, that side middle part fixedly connected with post one 505 that two clip 504 deviate from mutually, spring two 506 have been cup jointed in that one end outside of being close to clip 504 to post one 505, the one end fixedly connected clip 504 and the other end fixedly connected clip groove 503 of spring two 506.
The end, far away from the clamp 504, of the first column 505 penetrates through the side wall of the inner shell 502 and is fixedly connected with a movable cylinder 507, the movable cylinder 507 is located between the taking shell 501 and the inner shell 502, the movable cylinder 507 is in a transverse cylindrical shell shape, the end face, far away from the first column 505, of the movable cylinder 507 penetrates through and is sleeved with a second column 508, the end, close to the movable cylinder 507, of the second column 508 is inserted into the movable cylinder 507 and is fixedly connected with a first plate 509, the side, far away from the second column 508, of the first plate 509 is fixedly connected with a third spring 510, and the end, far away from the first plate 509, of the third spring 510 is fixedly connected with the inner wall of the movable cylinder 507.
A bottom groove 511 is formed in one end, close to the second column 508, of the lower side wall of the movable cylinder 507, a sliding block 512 is fixedly connected to the lower side wall of the first plate 509, the sliding block 512 is inserted into the bottom groove 511, the sliding block 512 can only slide in the bottom groove 511, a sliding rod 513 is fixedly connected to the lower end of the sliding block 512, and the second column 508 penetrates through the side wall of the taking shell 501 at the end, far away from the movable cylinder 507, and is fixedly connected with a handle 514.
The bottom plate device 6 is arranged at the lower port of the taking shell 501 and the lower port of the inner shell 502, the bottom plate device 6 comprises a bottom plate 601, the bottom plate 601 is annular, the inner ring of the bottom plate 601 is fixedly connected with the outer side wall of the lower port of the inner shell 502, the outer ring of the bottom plate 601 is fixedly connected with the inner side wall of the lower port of the taking shell 501, the left side and the right side of the surface of the bottom plate 601 are respectively provided with a second air hole 602, the second air hole 602 is opposite to a first air hole 402, the upper part of the inner wall of one side, away from the second air hole 602, of the side is provided with a side groove 603, an air plate 604 is inserted and sleeved in the side groove 603, one end, away from the side groove 603, of the air plate 604 extends out of the side groove 603, the shape of the air plate 604 is matched with the side groove 603, the upper surface, extending out of the side groove 603, corresponds to a sliding rod 513, the lower port of the second air hole 602 is fixedly connected with an insertion tube 605, and the insertion tube 605 is opposite to and matched with the first air hole 402.
The working principle is as follows:
the first step is as follows: the test tube filled with stem cells is clamped in the inner shell 502 by pressing the handle 514, the test tube filled with stem cells is vertically inserted into the placing shell 201, after the taking device 5, the bottom plate device 6, the cooling device 1, the placing device 2 and the top plate device 4 are contacted, the handle 514 is loosened, the test tube filled with stem cells enters the placing shell 201, the bottom of the test tube filled with stem cells is contacted with the bottom bowl 303, the test tube filled with stem cells presses the bottom bowl 303 down, the descending upper column 302 drives the first gear 305 to rotate anticlockwise, the second gear 307 rotates clockwise, the connecting ring 309 is driven to rotate clockwise, under the connection of the connecting plate 310, the blocking plate 311 rotates clockwise by taking the connecting ring 309 as an axis, the blocking plate 311 is moved out of the side hole 203, at the moment, the blocking plate 311 and the blocking belt 314 open the side hole 203, when the test tube filled with stem cells needs to be taken out, the direction of movement of the communicating device 3 is opposite, at this time, the blocking plate 311 and the blocking belt 314 reseal the side hole 203, and the device opens or closes the side hole 203 according to whether the test tube filled with stem cells is put in, thereby improving the self-adaptive performance of the device.
The second step is that: through the existence of gasbag 312, when the test tube that is equipped with stem cell entered into and places inside the shell 201, with the test tube bottom contact that is equipped with stem cell be gasbag 312 to make the test tube that is equipped with stem cell can not directly contact with shutoff board 311, and gasbag 312's self characteristic makes gasbag 312 have the ability of buffering, thereby help the protection test tube that is equipped with stem cell, improve the security of the test tube that is equipped with stem cell.
The third step: through the design of controlling two gasbag 312, when the test tube that is equipped with stem cell was put into, two gasbag 312 make the test tube bottom atress that is equipped with stem cell to center department to make just entering into when placing shell 201 inside at the test tube that is equipped with stem cell and fix a position for the test tube that is equipped with stem cell, make the test tube that is equipped with stem cell just can place bottom bowl 303, improved the location ability of device.
The fourth step: through the putting into of the test tube that is equipped with stem cell, thereby open side opening 203, fill the inside of cooling shell 101 with nitrogen gas from nitrogen gas valve 102, make the test tube that is equipped with stem cell by low temperature storage, after bottom plate device 6 and roof device 4 butt joint are accomplished, the inside nitrogen gas of cooling shell 101 also can enter into the inside of taking device 5, thereby make nitrogen gas only exist with being equipped with around the test tube of stem cell, like this when the test tube that is equipped with stem cell is deposited in the case, make nitrogen gas need not be full of the box completely inside through being equipped with this kind of device, the quantity of nitrogen gas that reduces, reduce the consumption to nitrogen gas.
The fifth step: when taking the test tube filled with the stem cells, when the bottom of the test tube filled with the stem cells is separated from the bottom bowl 303, due to the existence of the first spring 304, the bottom bowl 303 can restore to the original position, meanwhile, the first gear 305, the second gear 307, the connecting ring 309, the plugging plate 311 and the plugging belt 314 can restore to the original position, at the moment, the lateral hole 203 can be immediately plugged by the plugging plate 311 and the plugging belt 314, and meanwhile, the first plate 509 can drive the sliding rod 513 and the air plate 604 to close the second air hole 602 by the handle 514 pressed during taking, so that when the test tube filled with the stem cells is taken out, nitrogen is isolated inside the device, the nitrogen is prevented from leaking outwards to the maximum extent, and the nitrogen is saved.
And a sixth step: when a worker clamps a test tube filled with stem cells, the handle 514 needs to be pressed by hands from two sides, the clamp 504 clamps the upper end of the test tube filled with the stem cells in the inner shell 502, when the handle 514 is pressed, the first plate 509 can drive the sliding rod 513 to move in the bottom groove 511, so that the air plate 604 extends out of the second sealing air hole 602 from the side groove 603, when the test tube filled with the stem cells enters the placing shell 201 to be placed, the handle 514 is loosened, the second air hole 602 is communicated with the first air hole 402, part of nitrogen gas exists in the taking shell 501, and therefore when the test tube filled with the stem cells is taken subsequently, the test tube is taken by pressing the handle 514, so that the second air hole 602 is sealed continuously, the nitrogen gas slowly cools the test tube in the taking device 5, and the worker does not contact with the temperature of the nitrogen gas, make the test tube that is equipped with stem cell can be taken out by safety, and the temperature of the test tube that is equipped with stem cell can comparatively slow rising, avoid the test tube temperature of being equipped with stem cell to change sharply and lead to the test tube to explode and split.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. The utility model provides a full-automatic low temperature storage facilities of stem cell, includes heat sink (1), heat sink (1) is including cooling shell (101), the inside center department of cooling shell (101) is provided with placer (2), placer (2) are including placing shell (201), the last port of placing shell (201) flushes with the last port of cooling shell (101), the lower extreme of placing shell (201) is in the top of the lower extreme of cooling shell (101), its characterized in that: the lower extreme middle part of placing shell (201) is run through and has been seted up bottom hole (202), lateral wall all runs through and has been seted up side opening (203) about the middle part of placing shell (201), the inside lower extreme of cooling shell (101) is provided with intercommunication device (3), intercommunication device (3) include foundation column (301), the lower inner wall middle part of the lower extreme fixed connection cooling shell (101) of foundation column (301), upper column (302) has been cup jointed in the upper end activity of foundation column (301), the upper end of upper column (302) pass bottom hole (202) and with bottom hole (202) adaptation, the inside of placing shell (201) is stretched into to the upper end of upper column (302), upper end fixedly connected with bottom bowl (303) of upper column (302), bottom bowl (303) presents the bowl dress, the lower lateral wall fixedly connected with spring (304) of bottom bowl (303), the lower inner wall fixedly connected with of spring (304) places the lower extreme fixed connection of shell (201), the first spring (304) is wound on the outer side of the upper end of the upper column (302).
2. The fully automatic low temperature storage device for stem cells of claim 1, wherein: the upper portion of the front side of the cooling shell (101) is fixedly provided with a nitrogen valve (102), and the nitrogen valve (102) is communicated with the inside of the cooling shell (101).
3. The full-automatic low-temperature stem cell storage device according to claim 2, wherein: the outer side of the upper column (302) is in an annular bent shape, the lower end of the upper column (302) is located below the placing shell (201), the left side and the right side of the upper column (302) are respectively meshed with a first gear (305), the middle of the first gear (305) is connected with a first gear shaft (306) in a penetrating mode, the first gear shaft (306) is connected with the first gear (305) in a rotating mode through a bearing, the front end and the rear end of the first gear shaft (306) are fixedly connected with the inner side wall of the cooling shell (101), the first gear (305) is meshed with a second gear (307) on the side far away from the upper column (302), the middle of the second gear (307) is connected with a second gear shaft (308) in a penetrating mode, the second gear shaft (308) is connected with the second gear shaft (307) in a rotating mode through a bearing, and the front end and the rear end of the second gear shaft (308) are fixedly connected with the inner side wall of the cooling shell (101).
4. The full-automatic low-temperature stem cell storage device according to claim 3, wherein: the equal fixedly connected with go-between (309) in surface around gear two (307), go-between (309) cup joint in the outside of gear shaft two (308), two around being connected with same gear two (307) the last lateral wall of go-between (309) is connected with connecting plate (310) jointly, connecting plate (310) presents the arc plate shape, connecting plate (310) is in that one end fixedly connected with closure plate (311) of keeping away from go-between (309), closure plate (311) correspond insert in side opening (203) and with side opening (203) adaptation, closure plate (311) present the arc plate shape, the lower extreme of closure plate (311) and the lower inner wall contact of side opening (203), the upper end of closure plate (311) is passed side opening (203) and is stretched into the inside of putting shell (201), the upper end fixedly connected with gasbag one (312) of closure plate (311), the lower extreme fixedly connected with gasbag two (313) of closure plate (311), the edge fixedly connected with shutoff area (314) of the upper portion of shutoff board (311), shutoff area (314) have elasticity, shutoff area (314) wholly appear the U style of calligraphy, side opening (203) fixed connection is passed at that one end of keeping away from shutoff board (311) in shutoff area (314) on the inner wall of cooling shell (101), that the one end that shutoff area (314) and cooling shell (101) are connected is in the top of the one end of being connected with shutoff board (311), nitrogen gas valve (102) are in the top of shutoff area (314).
5. The full-automatic low-temperature stem cell storage device according to claim 1, wherein: the last port of cooling shell (101) and nitrogen gas valve (102) is provided with roof device (4) jointly, roof device (4) are including roof (401), roof (401) present the annular, the lateral wall of shell (201) is placed to the inner circle fixed connection of roof (401), the inside wall of the outer lane fixed connection cooling shell (101) of roof (401), gas pocket (402) have all been seted up to the upper surface left and right sides of roof (401), the inside embedding fixedly connected with cross opening pad (403) of gas pocket (402).
6. The full-automatic low-temperature stem cell storage device according to claim 1, wherein: the device for taking the nitrogen gas is characterized in that a taking device (5) is arranged above the cooling shell (101) and the nitrogen gas valve (102), the taking device (5) comprises a taking shell (501), the taking shell (501) is in an upper hemispherical shell shape, the diameter of the taking shell (501) is the same as that of the cooling shell (101), an inner shell (502) is arranged in the center of the inside of the taking shell (501), the inner shell (502) is in an upper semi-cylindrical shell shape, the diameter of the inner shell (502) is the same as that of the placing shell (201), the lower ports of the taking shell (501) and the inner shell (502) are flush, clamping grooves (503) are formed in the left side and the right side of the inner side wall of the inner shell (502), the clamping grooves (503) are symmetrical, clamping grooves (504) are embedded in the inside of the clamping grooves (503), the clamping grooves (504) are matched with the clamping grooves (503), and a first column (505) is fixedly connected to the middle of the side, which the clamping grooves (504) depart from each other, a second spring (506) is sleeved on the outer side of one end, close to the clamp (504), of the first column (505), one end of the second spring (506) is fixedly connected with the clamp (504), and the other end of the second spring is fixedly connected with the clamp groove (503).
7. The full-automatic low-temperature stem cell storage device according to claim 6, wherein: the first column (505) penetrates through the side wall of the inner shell (502) at the end far away from the clamp (504) and is fixedly connected with a movable cylinder (507), the movable cylinder (507) is located between the taking shell (501) and the inner shell (502), the movable cylinder (507) is in a transverse cylindrical shell shape, a second column (508) penetrates through the end face far away from the first column (505) of the movable cylinder (507), the second column (508) is inserted into the interior of the movable cylinder (507) at the end close to the movable cylinder (507) and is fixedly connected with a first plate (509), a third spring (510) is fixedly connected to the end far away from the second column (508) of the first plate (509), and the inner wall of the movable cylinder (507) is fixedly connected to the end far away from the first plate (509) of the third spring (510).
8. The full-automatic low-temperature stem cell storage device according to claim 7, wherein: bottom slot (511) have been seted up to the one end that the lower lateral wall of movable cylinder (507) is close post two (508), the lower lateral wall fixedly connected with slider (512) of board (509), slider (512) insert in bottom slot (511), the lower extreme fixedly connected with slide bar (513) of slider (512), the lateral wall and the fixedly connected with handle (514) of shell (501) of taking are run through to the one end that post two (508) kept away from movable cylinder (507).
9. The full-automatic low-temperature stem cell storage device according to claim 8, wherein: the taking shell (501) and the inner shell (502) are provided with bottom plate devices (6) at lower ports, each bottom plate device (6) comprises a bottom plate (601), each bottom plate (601) is annular, an inner ring of each bottom plate (601) is fixedly connected with the outer side wall of the lower port of the inner shell (502), an outer ring of each bottom plate (601) is fixedly connected with the inner side wall of the lower port of the taking shell (501), air holes II (602) are formed in the left side and the right side of the surface of each bottom plate (601), each air hole II (602) is right opposite to each air hole I (402), a side groove (603) is formed in the upper portion of the inner wall of one side, away from each air hole II (602), of the side grooves (603) is inserted and sleeved with an air plate (604), one end, away from each air plate (604) extends out of each side groove (603), each air plate (604) is matched with the shape of each side groove (603), the upper surface, extending out of each air plate (604) of each side groove (603), corresponds to each sliding rod (513), the lower port of the air hole II (602) is fixedly connected with an insertion tube (605), and the insertion tube (605) is opposite to and matched with the air hole I (402).
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| CN114514925B (en) | 2023-08-22 |
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