CN108378020B - Human stem cell refrigeration transportation method - Google Patents
Human stem cell refrigeration transportation method Download PDFInfo
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- CN108378020B CN108378020B CN201810209208.2A CN201810209208A CN108378020B CN 108378020 B CN108378020 B CN 108378020B CN 201810209208 A CN201810209208 A CN 201810209208A CN 108378020 B CN108378020 B CN 108378020B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0273—Transport containers
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Abstract
The invention relates to the technical field of cell storage, in particular to a human stem cell refrigerating and transporting method, which comprises a box body, a handle, a sealing mechanism, a control mechanism, a cover body, an opening mechanism, a freezing mechanism and an ejection mechanism, wherein the box body is in a box body structure, and the handle is arranged on the box body, so that the carrying is convenient; the box body is provided with a sealing mechanism which can be opened and closed at will through a control mechanism, and the use is convenient. The cover body and the freezing and storing mechanism are clamped in the groove in the box body, the cover body can be in adsorption connection with the box body through the second electromagnet after being inserted, the whole cover body and the test tube are popped out through the popping mechanism after the second electromagnet is closed by pressing the second button during taking, and the cover body and the test tube are more convenient and faster to put in, take and use; threaded connection between test tube and the lid, be equipped with seal gasket between the just, increase the leakproofness, prevent that the cell sap from leaking. Meanwhile, the diaphragm is sleeved outside the test tube, so that liquid nitrogen placed in the first accommodating cavity between the diaphragm and the box body can be effectively prevented from leaking, and safety is guaranteed.
Description
Technical Field
The invention relates to the technical field of cell storage, in particular to a human stem cell refrigeration transportation method.
Background
Stem cells are a type of pluripotent cells that have the ability to self-replicate. Under certain conditions, it can differentiate into a variety of functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. The stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent stem cells. Stem cells are insufficiently differentiated and immature cells, have potential functions of regenerating various tissues, organs and human bodies, and are called universal cells in the medical field. Stem cells are generally stored using liquid nitrogen at-196 ℃.
In the process of transporting stem cells, the stem cells are inconvenient to carry and take and are inconvenient to use, and the existing refrigerated transport case can not well protect the stem cells. In view of the above, the present invention provides a human stem cell refrigeration transportation method, which has the following characteristics:
the human stem cell refrigeration transportation method adopts a box body structure to refrigerate human stem cells, and is convenient to carry and transport.
2 the human stem cell refrigerating and transporting method of the invention, the box body is provided with the sealing mechanism, the sealing mechanism is opened and closed at will through the control mechanism, and the first sealing plate and the second sealing plate do not occupy the space of the whole box body in the horizontal or vertical direction after being opened, thus the use is convenient.
3 according to the human stem cell refrigerating and transporting method, the cover body and the refrigerating and storing mechanism are clamped in the groove in the box body, the cover body can be in adsorption connection with the box body through the second electromagnet after being inserted, the whole cover body and the test tube are ejected through the ejection mechanism after the second electromagnet is closed by pressing the second button during taking, and the human stem cell refrigerating and transporting method is more convenient and faster to put in, take and use.
4 the invention relates to a human stem cell refrigeration transportation method, wherein a test tube is in threaded connection with a cover body, and a sealing gasket is arranged between the test tube and the cover body, so that the sealing property is improved, and the leakage of cell sap is prevented. Meanwhile, the diaphragm is sleeved outside the test tube, so that liquid nitrogen placed in the first accommodating cavity between the diaphragm and the box body can be effectively prevented from leaking, and safety is guaranteed.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a human stem cell refrigerating and transporting method, which adopts a box body structure, and a handle is arranged on the box body, so that the carrying is convenient; be equipped with sealing mechanism on the box, sealing mechanism opens at will through control mechanism and closes, and first closing plate and second closing plate do not occupy the space of whole box level or vertical direction after opening, convenient to use. The cover body and the freezing and storing mechanism are clamped in the groove in the box body, the cover body can be in adsorption connection with the box body through the second electromagnet after being inserted, the whole cover body and the test tube are popped out through the popping mechanism after the second electromagnet is closed by pressing the second button during taking, and the cover body and the test tube are more convenient and faster to put in, take and use; threaded connection between test tube and the lid, the pad is equipped with seal gasket between and, increases the leakproofness, prevents that the cell sap from leaking. Meanwhile, the diaphragm is sleeved outside the test tube, so that liquid nitrogen placed in the first accommodating cavity between the diaphragm and the box body can be effectively prevented from leaking, and safety is guaranteed.
The technical scheme adopted by the invention for solving the technical problems is as follows: a human stem cell refrigeration transportation method adopts a refrigeration box for refrigeration, the refrigeration box comprises a box body, a handle, a sealing mechanism, a control mechanism, a cover body, an opening mechanism, a freezing mechanism and an ejection mechanism, and the handle is arranged on one side of the box body; the sealing mechanism is arranged on the box body and comprises a first sealing plate, a second sealing plate, a telescopic column, a convex block, a baffle plate, a first spring, a first electromagnet and a lead, the first sealing plate and the second sealing plate are connected in a groove on the top surface of the box body in a sliding clamping manner, the convex block is arranged on two sides of the box body, the convex block is connected in a sliding groove on the box body in a sliding clamping manner, the first sealing plate and the second sealing plate are fixedly connected through the telescopic column in a clamping manner, the part of the telescopic column penetrating into the first sealing plate is sleeved with the baffle plate and the first spring, one end of the first spring is connected with the baffle plate, the other end of the first spring is fixedly connected in the first sealing plate, and the tail end of the telescopic column is connected with the first electromagnet in a magnetic adsorption manner, the first electromagnetic iron is electrically connected with the lead; the control mechanism is arranged on the box body and positioned on the same side of the handle; the cover body is arranged on the box body and positioned at the bottom of the first sealing plate and the second sealing plate; the cover body is provided with the opening mechanism, the opening mechanism comprises a second electromagnet, a second spring, a second button, a first copper sheet, a second copper sheet, a third copper sheet, a fourth copper sheet and a fifth copper sheet, the second button is connected to the top end of the cover body in a sliding and telescopic manner, the second electromagnet is arranged on two sides of the cover body in the box body, the second electromagnet is in ferromagnetic adsorption connection with the second button, the cover body is in telescopic connection with the second button, the bottom end of the second button is connected with the second spring, the second spring is fixedly connected to the bottom of the inner cavity of the cover body, two sides of the second button are provided with the first copper sheets, the cover body is provided with the second copper sheet and the third copper sheet which are in contact connection with the first copper sheet, the side wall of the inner cavity of the box body is provided with the fourth copper sheet and the fifth copper sheet which are in contact connection with the second copper sheet and the third copper sheet, the fourth copper sheet and the fifth copper sheet are electrically connected with the second electromagnet; the ejection mechanism is arranged at the bottom of the cover body in the box body and comprises an elastic plate and a third spring, the top surface of the elastic plate is in contact connection with the edge of the bottom surface of the cover body, the bottom end of the elastic plate is connected in the box body in a telescopic mode, the tail end of the elastic plate is connected with the third spring, and the third spring is connected in the box body; the freezing and storing mechanism connected with the cover body is arranged in the box body;
the refrigeration method comprises the following steps:
the first step is as follows: putting the obtained human stem cells into a freezing and storing mechanism for storage;
the second step is that: refrigerating the human stem cells stored in the first step in a box body, and placing liquid nitrogen in the box body;
the third step: sealing the box body in the second step through a sealing mechanism to ensure the stability of the temperature in the box body;
the fourth step: and when the human stem cells refrigerated in the third step are taken out, ejecting the freezing and storing mechanism through an ejecting mechanism.
Specifically, the spout on the box is "back" shape structure, first closing plate with the size of second closing plate equals, just the height of spout does the twice of the thickness of first closing plate, the lug is the square structure, just the width of lug with on the box the width of spout equals, realizes can alternately cyclic shift between first closing plate and the second closing plate, and removes the in-process, does not take place the skew of position, reaches effective spacing effect.
Specifically, the lug is made for having the material of electric conductivity, first electro-magnet passes through wire electric connection the lug, first closing plate one side sets up flexible post reaches first electro-magnet is equipped with a plurality ofly, and a plurality ofly pass through between the first electro-magnet the wire is parallelly connected, realizes utilizing control mechanism can effective control flexible of post is flexible, realizes that the block between first closing plate and the second closing plate is fixed, and is a plurality of flexible simultaneously between the flexible post, the time is unanimous.
Specifically, control mechanism includes first button, rotation post, shell fragment and graphite coating, first button locate with the handle is with one side on the box, first button cup joint in on the rotation post, the both sides contact of first button connect in the shell fragment, the bottom surface one end contact of first button connect in the graphite coating, just the graphite coating is located on the box the inside wall of spout has realized through pressing first button, control among the sealing mechanism flexible post is realized opening or is connected first closing plate with the second closing plate.
Specifically, the first button is of a parallelogram structure with two arc-shaped sides, the first button is in contact connection with one side of the elastic sheet and is arc-shaped, the rotating column is arranged at the central point of the first button, the two ends of the first button are pressed, and the first button swings back and forth fixedly.
Specifically, the height of the first copper sheet is equal to the distance between the second copper sheet and the third copper sheet and the distance between the fourth copper sheet and the fifth copper sheet, and the top surface of the second button and the top surface of the cover body are located on the same horizontal plane.
Specifically, the elastic plate is of a T-shaped structure, the maximum compression distance of the third spring is equal to the distance between the bottom surface of the elastic plate and the box body, the top surface of the cover body is located on the same horizontal plane with the top surface of the box body, and the cover body is effectively ejected out.
Specifically, it includes test tube, diaphragm and seal gasket to freeze to hide the mechanism, test tube threaded connection in the bottom of lid, the lid with fill up between the test tube seal gasket, the bottom of test tube has cup jointed the diaphragm, just diaphragm fixed connection in the box, just the box with form first accommodation cavity A between the diaphragm, the intraductal second that is equipped with holds cavity A, realizes the lid with sealing connection between the test tube prevents in the test tube the second holds cavity A well cell liquid and leaks, and is simultaneously profitable the test tube cup joints outward the diaphragm effectively prevents that the liquid nitrogen from leaking, causes the scald.
The invention has the beneficial effects that:
the human stem cell refrigeration transportation method adopts a box body structure, and a handle is arranged on the box body, so that the human stem cell refrigeration transportation method is convenient to carry.
2 the human stem cell refrigerating and transporting method of the invention, the box body is provided with the sealing mechanism, the sealing mechanism is opened and closed at will through the control mechanism, and the first sealing plate and the second sealing plate do not occupy the space of the whole box body in the horizontal or vertical direction after being opened, thus the use is convenient.
3 according to the human stem cell refrigerating and transporting method, the cover body and the refrigerating and storing mechanism are clamped in the groove in the box body, the cover body can be in adsorption connection with the box body through the second electromagnet after being inserted, the whole cover body and the test tube are ejected through the ejection mechanism after the second electromagnet is closed by pressing the second button during taking, and the human stem cell refrigerating and transporting method is more convenient and faster to put in, take and use.
4 the invention relates to a human stem cell refrigeration transportation method, wherein a test tube is in threaded connection with a cover body, and a sealing gasket is arranged between the test tube and the cover body, so that the sealing property is improved, and the leakage of cell sap is prevented. Meanwhile, the diaphragm is sleeved outside the test tube, so that liquid nitrogen placed in the first accommodating cavity between the diaphragm and the box body can be effectively prevented from leaking, and safety is guaranteed.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic structural diagram of a preferred embodiment of a stem cell storage and carrying device according to the present invention;
FIG. 2 is a schematic view of a connecting mechanism of the case and the cover shown in FIG. 1;
FIG. 3 is a schematic cross-sectional view of the lid and the freezing mechanism shown in FIG. 1;
FIG. 4 is a schematic cross-sectional view of the first seal plate and bump shown in FIG. 1;
FIG. 5 is a schematic cross-sectional view of the sealing mechanism shown in FIG. 1;
fig. 6 is an enlarged view of the portion a shown in fig. 3.
In the figure: 1. the box body comprises a box body 1A, a sliding groove 1B, a first accommodating cavity 2, a handle 3, a sealing mechanism 31, a first sealing plate 32, a second sealing plate 33, a telescopic column 34, a bump 35, a baffle 36, a first spring 37, a first electromagnet 38, a lead wire 4, a control mechanism 41, a first button 42, a rotating column 43, an elastic sheet 44, a graphite coating 5, a cover body 6, an opening mechanism 61, a second electromagnet 62, a second spring 63, a second button 64, a first copper sheet 65, a second copper sheet 66, a third copper sheet 67, a fourth copper sheet 68, a fifth copper sheet 7, a freezing and storing mechanism 71, a test tube 71A, a second accommodating cavity 72, a diaphragm 73, a sealing gasket 8, a popping mechanism 81, a springboard 82 and a third spring.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1, the human stem cell refrigeration transportation method of the present invention adopts a refrigeration box for refrigeration, the refrigeration box comprises a box body 1, a handle 2, a sealing mechanism 3, a control mechanism 4, a cover body 5, an opening mechanism 6, a freezing mechanism 7 and an ejecting mechanism 8, wherein the handle 2 is arranged on one side of the box body 1; the sealing mechanism 3 is arranged on the box body 1, the sealing mechanism 3 includes a first sealing plate 31, a second sealing plate 32, a telescopic column 33, a projection 34, a baffle 35, a first spring 36, a first electromagnet 37 and a lead 38, the first sealing plate 31 and the second sealing plate 32 are connected in a groove on the top surface of the box body 1 in a sliding and clamping manner, the projection 34 is arranged on two sides of the box body 1 to connect the first sealing plate 31 and the second sealing plate 32, the projection 34 is connected in a sliding and clamping manner in a sliding groove 1A on the box body 1, the first sealing plate 31 and the second sealing plate 32 are fixedly connected in a clamping manner through the telescopic column 33, the baffle 35 and the first spring 36 are sleeved on a part of the telescopic column 33 penetrating into the first sealing plate 31, and one end of the first spring 36 is connected to the baffle 35, the other end of the first spring 36 is fixedly connected in the first sealing plate 31, the tail end of the telescopic column 33 is magnetically connected with the first electromagnet 37, and the first electromagnet 37 is electrically connected to the lead 38; the control mechanism 4 is arranged on the box body 1 and positioned on the same side of the handle 2; the cover body 5 is arranged at the bottom of the first sealing plate 31 and the second sealing plate 32 on the box body 1; the opening mechanism 6 is arranged on the cover body 5, the opening mechanism 6 comprises a second electromagnet 61, a second spring 62, a second button 63, a first copper sheet 64, a second copper sheet 65, a third copper sheet 66, a fourth copper sheet 67 and a fifth copper sheet 68, the second button 63 is slidably and telescopically connected to the top end of the cover body 5, the second electromagnet 61 is arranged on two sides of the cover body 5 in the box body 1, the second electromagnet 61 is magnetically adsorbed and connected to the second button 63, the cover body 5 is telescopically connected to the second button 63, the bottom end of the second button 63 is connected to the second spring 62, the second spring 62 is fixedly connected to the bottom of the inner cavity of the cover body 5, the first copper sheet 64 is arranged on two sides of the second button 63, the cover body 5 is provided with the second copper sheet 65 and the third copper sheet 66 which are in contact connection with the first copper sheet 64, the side wall of the inner cavity of the box body 1 is provided with the fourth copper sheet 67 and the fifth copper sheet 68 which are in contact connection with the second copper sheet 65 and the third copper sheet 66, and the fourth copper sheet 67 and the fifth copper sheet 68 are electrically connected with the second electromagnet 61; the ejection mechanism 8 is arranged at the bottom of the cover body 5 in the box body 1, the ejection mechanism 8 comprises an elastic plate 81 and a third spring 82, the top surface of the elastic plate 81 is in contact connection with the edge of the bottom surface of the cover body 5, the bottom end of the elastic plate 81 is in telescopic connection with the inside of the box body 1, the tail end of the elastic plate 81 is connected with the third spring 82, and the third spring 82 is connected with the inside of the box body 1; the freezing and storing mechanism 7 connected with the cover body 5 is arranged in the box body 1;
the refrigeration method comprises the following steps:
the first step is as follows: the obtained human stem cells are put into a freezing and storing mechanism 7 for storage;
the second step is that: placing the human stem cells stored in the first step into a box body 1 for refrigeration, and placing liquid nitrogen into the box body 1;
the third step: sealing the box body 1 in the second step through a sealing mechanism 3 to ensure the stability of the temperature in the box body 1;
the fourth step: and when the human stem cells refrigerated in the third step are taken out, the freezing and storing mechanism 7 is ejected out through the ejection mechanism 8.
Specifically, as shown in fig. 2 and 4, the chute 1A on the box body 1 is of a "return" structure, the first sealing plate 31 and the second sealing plate 32 are equal in size, the height of the chute 1A is twice the thickness of the first sealing plate 31, the protrusion 34 is of a square structure, and the width of the protrusion 34 is equal to the width of the chute 1A on the box body 1, so that the first sealing plate 31 and the second sealing plate 32 can move in a cross-cycling manner, and in the moving process, no position deviation occurs, and an effective limiting effect is achieved.
Specifically, as shown in fig. 5, the bump 34 is made of a conductive material, the first electromagnet 37 is electrically connected to the bump 34 through the wire 38, the telescopic column 33 and the first electromagnets 37 arranged on one side of the first sealing plate 31 are provided in plurality, and the first electromagnets 37 are connected in parallel through the wire 38, so that the telescopic column 33 can be effectively controlled to be telescopic by using the control mechanism 4, the first sealing plate 31 and the second sealing plate 32 can be clamped and fixed, and the telescopic columns 33 can be simultaneously telescopic and have the same time.
Specifically, as shown in fig. 5, the control mechanism 4 includes a first button 41, a rotating post 42, a resilient plate 43, and a graphite coating 44, the first button 41 is disposed on the box body 1 on the same side as the handle 2, the first button 41 is sleeved on the rotating post 42, two sides of the first button 41 are connected to the resilient plate 43 in a contact manner, one end of the bottom surface of the first button 41 is connected to the graphite coating 44 in a contact manner, and the graphite coating 44 is disposed on the inner side wall of the chute 1A on the box body 1, so that the first button is pressed to control the telescopic post 33 in the sealing mechanism 3 to be telescopic, and the first sealing plate 31 and the second sealing plate 32 are opened or connected.
Specifically, as shown in fig. 5, the first button 41 is of a parallelogram structure with two arc-shaped sides, the first button 41 is in contact connection with one side of the elastic sheet 43 and is in an arc shape, the rotating column 42 is arranged at the central point of the first button 41, so that the two ends of the first button 41 are pressed, and the first button 41 swings back and forth and is fixed.
Specifically, as shown in fig. 3, the height of the first copper sheet 64 is equal to the distance between the second copper sheet 65 and the third copper sheet 66 and the distance between the fourth copper sheet 67 and the fifth copper sheet 68, and the top surface of the second button 63 and the top surface of the cover 5 are on the same horizontal plane, so as to achieve more precise control, after the second button 63 is pressed, the connection circuit is disconnected, and the cover 5 is ejected by the ejection mechanism 8.
Specifically, as shown in fig. 6, the elastic plate 81 is of a "T" shape, and the maximum compression distance of the third spring 82 is equal to the distance between the bottom surface of the elastic plate 81 and the box body 1, and the top surface of the cover 5 and the top surface of the box body 1 are located on the same horizontal plane, so that the cover 5 is effectively ejected.
Specifically, as shown in fig. 3, the frozen storage mechanism 7 includes a test tube 71 and a diaphragm 72, the test tube 71 is screwed in the bottom of the cover body 5, the diaphragm 72 is sleeved at the bottom of the test tube 71, the diaphragm 72 is fixedly connected to the inside of the box body 1, a first accommodating cavity 71A is formed between the box body 1 and the diaphragm 72, a second accommodating cavity 1B is arranged in the test tube 71, so that the cover body 5 and the test tube 71 are hermetically connected, the leakage of cellular liquid in the second accommodating cavity 71A in the test tube 71 is prevented, and meanwhile, the diaphragm 72 sleeved outside the test tube 71 is utilized, so that the leakage of liquid nitrogen and the scalding are effectively prevented.
The cell sap is stored in the test tube 71 by the freezing mechanism 7, and then sealed by the cover 5 to prevent the cell sap from leaking, and simultaneously the test tube 71 and the cover 5 are inserted into the case 1, and the cell sap in the test tube 71 is frozen by the liquid nitrogen provided in the first accommodation cavity 1B formed between the diaphragm 72 and the case 1 by the fixed connection of the second electromagnet 61. Utilize sealing mechanism 3 to realize effective sealed, utilize control mechanism 4 control sealing mechanism 3 to open or close, utilize the handle 2 that box 1 one side set up to carry. The method specifically comprises the following steps:
(1) the cell sap is put into the test tube 71, then the test tube 71 is connected with the cover body 5 in a threaded manner, then the cover body 5 and the test tube 71 are inserted into the box body 1, the elastic plate 81 and the third spring 82 are compressed, meanwhile, the first copper sheet 64, the second copper sheet 65, the third copper sheet 66, the fourth copper sheet 67 and the fifth copper sheet 68 are communicated, and the second electromagnet 61 is opened, so that the cover body 5 and the box body 1 are fixedly connected in an absorption manner. At this time, the diaphragm 72 in the box body 1 is sleeved outside the test tube 71, and liquid nitrogen is placed in the first accommodating cavity 1B between the diaphragm 72 and the box body 1, so that the cell sap in the test tube 71 is effectively frozen and stored.
(2) The first sealing plate 31 and the second sealing plate 32 are moved, the first sealing plate 31 and the second sealing plate 32 are connected with the chute 1A in a 'return' shape in a sliding and clamping mode through the convex blocks 34 arranged on the side walls of the first sealing plate 31 and the second sealing plate 32, the first sealing plate 31 and the second sealing plate 32 are located on the same horizontal plane of the top surface of the cover body 5, then the first button 41 is pressed and fixed through the clamping of the elastic sheets 43, the graphite coating 44 is connected through the contact of the two ends of the first button 41, a circuit from the lead 38, the convex blocks 34 and the graphite coating 44 to the first button 41 is communicated, the first electromagnet 37 is opened and closed, the telescopic column 33 is connected through the adsorption of the first electromagnet 37 or the telescopic column 33 is popped out through the first spring 36, the telescopic column 33 is stretched, and the first sealing plate 31 and the.
(3) Then utilize the handle 2 that one side set up on the box 1 to lift up the box 1, conveniently carry, press first button 41 and can open first closing plate 31 and second closing plate 32 when taking simultaneously, then press second button 63, make first copper sheet 64 break away from second copper sheet 65, close second electro-magnet 61, remove the absorption between box 1 and the lid 5 and be connected, can utilize elastic plate 81 and third spring 82 to pop out test tube 71 and lid 5, conveniently take.
The portable multifunctional portable suitcase adopts a suitcase body structure, and the suitcase body 1 is provided with the handle 2, so that the portable multifunctional suitcase is convenient to carry. Be equipped with sealing mechanism 3 on the box 1, sealing mechanism 3 opens and closes at will through control mechanism 4, and opens the back first closing plate 31 and second closing plate 32 and do not occupy the space of whole box 1 level or vertical direction, convenient to use. Block lid 5 and the mechanism 7 of hiding that freezes in the recess in the box 1, lid 5 can adsorb through second electro-magnet 61 and box 1 after inserting and be connected, utilizes pop-up mechanism 8 to pop out whole lid 5 and test tube 71 after closing second electro-magnet 61 through pressing down second button 63 when taking, puts into, takes and uses all convenient and fast more. The screw connection between the test tube 71 and the cover body 5 increases the sealing performance and prevents leakage of cell sap. Meanwhile, the membrane 72 is sleeved outside the test tube 71, so that liquid nitrogen placed in the first accommodating cavity 1B between the membrane 72 and the box body 1 can be effectively prevented from leaking, and safety is guaranteed.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the embodiments and descriptions given above are only illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A human stem cell refrigeration transportation method is characterized in that: the method adopts a refrigerating box for refrigerating, the refrigerating box comprises a box body (1), a handle (2), a sealing mechanism (3), a control mechanism (4), a cover body (5), an opening mechanism (6), a refrigerating mechanism (7) and an ejecting mechanism (8), and the handle (2) is arranged on one side of the box body (1); be equipped with on box (1) sealing mechanism (3), sealing mechanism (3) include first sealing plate (31), second sealing plate (32), flexible post (33), lug (34), baffle (35), first spring (36), first electro-magnet (37) and wire (38), first sealing plate (31) with second sealing plate (32) sliding clamping connect in the recess on box (1) top surface, first sealing plate (31) with second sealing plate (32) connect in the both sides of box (1) are equipped with lug (34), just lug (34) sliding clamping connect in spout (1A) on box (1), pass through between first sealing plate (31) and the second sealing plate (32) flexible post (33) block fixed connection, flexible post (33) penetrate the part in first sealing plate (31) cup joints baffle (35) with first sealing plate (31) A spring (36), wherein one end of the first spring (36) is connected to the baffle plate (35), the other end of the first spring (36) is fixedly connected to the inside of the first sealing plate (31), the tail end of the telescopic column (33) is magnetically connected with the first electromagnet (37), and the first electromagnet (37) is electrically connected to the lead (38);
the control mechanism (4) is arranged on the box body (1) and positioned on the same side of the handle (2); the cover body (5) is arranged at the bottom of the first sealing plate (31) and the second sealing plate (32) on the box body (1); the opening mechanism (6) is arranged on the cover body (5), the opening mechanism (6) comprises a second electromagnet (61), a second spring (62), a second button (63), a first copper sheet (64), a second copper sheet (65), a third copper sheet (66), a fourth copper sheet (67) and a fifth copper sheet (68), the second button (63) is connected to the top end of the cover body (5) in a sliding and telescopic manner, the second electromagnet (61) is arranged on two sides of the cover body (5) in the box body (1), the second electromagnet (61) is connected to the second button (63) in a magnetic adsorption manner, the second button (63) is connected to the cover body (5) in a telescopic manner, the bottom end of the second button (63) is connected to the second spring (62), and the second spring (62) is fixedly connected to the bottom of the inner cavity of the cover body (5), the first copper sheets (64) are arranged on two sides of the second button (63), the second copper sheets (65) and the third copper sheets (66) which are in contact connection with the first copper sheets (64) are arranged on the cover body (5), the fourth copper sheets (67) and the fifth copper sheets (68) which are in contact connection with the second copper sheets (65) and the third copper sheets (66) are arranged on the side wall of the inner cavity of the box body (1), and the fourth copper sheets (67) and the fifth copper sheets (68) are electrically connected with the second electromagnet (61);
the ejection mechanism (8) is arranged at the bottom of the cover body (5) in the box body (1), the ejection mechanism (8) comprises an elastic plate (81) and a third spring (82), the top surface of the elastic plate (81) is in contact connection with the edge of the bottom surface of the cover body (5), the bottom end of the elastic plate (81) is in telescopic connection with the box body (1), the tail end of the elastic plate (81) is connected with the third spring (82), and the third spring (82) is connected with the box body (1); the box body (1) is internally provided with the freezing and storing mechanism (7) connected with the cover body (5);
the refrigeration method comprises the following steps:
the first step is as follows: the obtained human stem cells are put into a freezing and storing mechanism (7) for storage;
the second step is that: refrigerating the human stem cells stored in the first step in a box body (1), wherein liquid nitrogen is placed in the box body (1);
the third step: sealing the box body (1) in the second step through a sealing mechanism (3) to ensure the stability of the temperature in the box body (1);
the fourth step: and when the human stem cells refrigerated in the third step are taken out, the freezing and storing mechanism (7) is ejected out through an ejection mechanism (8).
2. The method for transporting stem cells of human body in a refrigerated manner according to claim 1, wherein: the novel sealing box is characterized in that the sliding groove (1A) in the box body (1) is of a 'return' structure, the first sealing plate (31) and the second sealing plate (32) are equal in size, the height of the sliding groove (1A) is twice the thickness of the first sealing plate (31), the bump (34) is of a cube structure, and the width of the bump (34) is equal to the width of the sliding groove (1A) in the box body (1).
3. The method for transporting stem cells of human body in a refrigerated manner according to claim 1, wherein: the bump (34) is made of a conductive material, the first electromagnet (37) is electrically connected with the bump (34) through the lead (38), the telescopic column (33) and the first electromagnet (37) are arranged on one side of the first sealing plate (31), and the first electromagnets (37) are connected in parallel through the lead (38).
4. The method for transporting stem cells of human body in a refrigerated manner according to claim 1, wherein: control mechanism (4) include first button (41), rotate post (42), shell fragment (43) and graphite coating (44), first button (41) locate with handle (2) are with one side on box (1), first button (41) cup joint in rotate on post (42), the both sides contact of first button (41) connect in shell fragment (43), the bottom surface one end contact of first button (41) connect in graphite coating (44), just graphite coating (44) are located on box (1) the inside wall of spout (1A).
5. The method for transporting stem cells of human body in a refrigerated manner according to claim 4, wherein: the first button (41) is of a parallelogram structure with two arc-shaped sides, the first button (41) is in contact connection with one side of the elastic sheet (43) and is arc-shaped, and the rotating column (42) is arranged at the central point of the first button (41).
6. The method for transporting stem cells of human body in a refrigerated manner according to claim 1, wherein: the height of the first copper sheet (64) is equal to the distance between the second copper sheet (65) and the third copper sheet (66) and the distance between the fourth copper sheet (67) and the fifth copper sheet (68), and the top surface of the second button (63) and the top surface of the cover body (5) are on the same horizontal plane.
7. The method for transporting stem cells of human body in a refrigerated manner according to claim 1, wherein: the elastic plate (81) is of a T-shaped structure, the maximum compression distance of the third spring (82) is equal to the distance between the bottom surface of the elastic plate (81) and the box body (1), and the top surface of the cover body (5) and the top surface of the box body (1) are located on the same horizontal plane.
8. The method for transporting stem cells of human body in a refrigerated manner according to claim 1, wherein: it includes test tube (71), diaphragm (72) to freeze and hide mechanism (7), test tube (71) threaded connection in the bottom of lid (5), the bottom of test tube (71) has been cup jointed diaphragm (72), just diaphragm (72) fixed connection in box (1), just box (1) with form first accommodation cavity (1B) between diaphragm (72), be equipped with the second in test tube (71) and hold cavity (71A).
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| CN109568619A (en) * | 2019-01-15 | 2019-04-05 | 马遥 | A kind of anti-infection isolating device of Pediatric Clinic medical use |
| CN114559677B (en) * | 2022-03-01 | 2023-09-12 | 无锡国盛生物工程股份有限公司 | ELISA plate for preventing cross contamination of ELISA plate holes and production process thereof |
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