CN114504516A

CN114504516A - Freckle-removing whitening cream

Info

Publication number: CN114504516A
Application number: CN202210193848.5A
Authority: CN
Inventors: 曲中周
Original assignee: Guozhuang Guangzhou Technology Co ltd
Current assignee: Guozhuang Guangzhou Technology Co ltd
Priority date: 2022-03-01
Filing date: 2022-03-01
Publication date: 2022-05-17

Abstract

The invention discloses a freckle-removing whitening cream, which comprises the following components: 50-65 parts of water, 3-6 parts of nicotinamide, 0.1-1 part of tranexamic acid, 0.5-3 parts of lactuca sativa polyphenol, 1-3 parts of 4-butylresorcinol, 0.1-1 part of azelaic acid, 0.1-0.5 part of bisabolol, 8-15 parts of an emollient, 5-8 parts of an emulsifier, 6-10 parts of a humectant, 0.5-2 parts of a thickener, 0.5-1 part of an antioxidant, 0.1-0.5 part of an astringent, 0.1-5 parts of a skin conditioner, 0.01-0.05 part of an essence, 0.1-0.7 part of a preservative, 0.002-0.005 part of a colorant, 0.2-0.45 part of a sun-screening agent, 0.1-0.6 part of a pH regulator and 0.01-0.05 part of a chelating agent. According to the invention, the lactuca indica polyphenol, tranexamic acid and nicotinamide are used as active ingredients for whitening and removing freckles, so that a cream product with good whitening and freckles removing effects is obtained.

Description

Freckle-removing whitening cream

Technical Field

The invention relates to the field of cosmetics, in particular to spot-removing whitening cream.

Background

Melanin, also known as Mailanin pigment, is commonly found in animals and plants, and is secreted by melanocytes in the basal layer of the skin, where an amino acid (tyrosine) in melanocytes produces a chemical change when stimulated to produce secreted melanin. For example, when the ultraviolet rays in the sunlight irradiate the skin, the chemical action of the ultraviolet rays can change the tyrosine in the melanin to form tyrosinase which is secreted into the skin to absorb the short-wave ultraviolet rays which can cause inflammation, and then the short-wave ultraviolet rays fall off along with keratodermia by virtue of the metabolism or enter a blood circulation system to be discharged out of the body; however, if the exposure time is too long, the exposure amount is too large, and the melanin cannot be removed from the skin at a proper time, the melanin precipitates in the skin, the uniform precipitation causes the skin to appear black, and the uneven color lump precipitates to form black spots or darken freckles.

With the improvement of the living standard of people at present, more and more women pay attention to the appearance of the women, and for the impurity spots on the face, whitening cream with the spot removing function is used for whitening and removing spots.

Disclosure of Invention

The invention aims to provide the spot-removing whitening cream which has small damage to skin and good whitening and spot-removing effects.

The purpose of the invention is realized by adopting the following technical scheme:

the spot-removing whitening cream comprises the following components in parts by weight:

50-65 parts of water, 3-6 parts of nicotinamide, 0.1-1 part of tranexamic acid, 0.5-3 parts of lactuca sativa polyphenol, 1-3 parts of 4-butylresorcinol, 0.1-1 part of azelaic acid, 0.1-0.5 part of bisabolol, 8-15 parts of an emollient, 5-8 parts of an emulsifier, 6-10 parts of a humectant, 0.5-2 parts of a thickener, 0.5-1 part of an antioxidant, 0.1-0.5 part of an astringent, 0.1-5 parts of a skin conditioner, 0.01-0.05 part of an essence, 0.1-0.7 part of a preservative, 0.002-0.005 part of a colorant, 0.2-0.45 part of a sun-screening agent, 0.1-0.6 part of a pH regulator and 0.01-0.05 part of a chelating agent.

Preferably, the emollient is at least one of isononyl isononanoate, ethylhexyl palmitate, ethylhexyl stearate, polydimethylsiloxane, cyclopentadimethylsiloxane, cyclohexasiloxane, beeswax, polydimethylsiloxane crosspolymer.

Preferably, the emulsifier is at least one of glyceryl stearate, potassium cetyl phosphate, cetyl alcohol, PEG-100 stearate, ceteareth-20, PEG-75 stearate.

Preferably, the humectant is at least one of propylene glycol, betaine, trehalose, butylene glycol, 1, 3-propylene glycol, 1, 2-pentanediol, 1, 2-hexanediol, and ethylhexylglycerin.

Preferably, the thickening agent is at least one of acrylic acid/C10-30 alkanol acrylate cross-linked polymer, acrylate/C10-30 alkanol acrylate cross-linked polymer, polyacrylamide, C13-14 isoparaffin, laureth-7 and xanthan gum.

Preferably, the antioxidant is at least one of tocopherol acetate, sodium metabisulfite and butylated hydroxytoluene.

Preferably, the skin conditioner is at least one of dogwood extract, ginkgo biloba extract, peony root extract, saffron extract and resveratrol.

Preferably, the preservative is at least one of methylparaben, propylparaben, o-cymene-5-ol, CI19140, CI 16255.

Preferably, the astringent is allantoin; the sun-screening agent is ethylhexyl methoxycinnamate; the pH regulator is triethanolamine; the chelating agent is disodium EDTA.

Preferably, the lactuca sativa polyphenol is extracted from the rhizomes of lactuca sativa.

Preferably, the method for extracting the lactuca sativa polyphenol comprises the following steps:

step 1, picking fresh wild lettuce, removing roots and stems of the wild lettuce from leaves of the wild lettuce, cleaning the surfaces of the roots, draining, cutting into blocks to obtain wild lettuce blocks, and performing fresh-keeping treatment for later use;

step 2, placing the lettuce blocks in a vacuum drying oven, and drying to remove water to obtain dried lettuce;

step 3, smashing the lactuca sativa into powder, and treating the powder in a superfine pulverizer to obtain lactuca sativa powder;

step 4, placing the lactuca indica powder in an ethanol solution for extraction treatment to obtain a first extracting solution and first filter residue;

step 5, treating the first filter residue in alkali liquor, then dropwise adding acid liquor to adjust the pH value to be less than 2, then centrifuging and collecting supernate, and extracting and redissolving to obtain a second extracting solution;

and 6, combining the first extracting solution and the second extracting solution, and evaporating under reduced pressure until the mixture is completely dried to obtain the lactuca sativa polyphenol.

Preferably, the blocks in the step 1 are cut into pieces with volume size of 8-12 mm³。

Preferably, in the step 1, the temperature of the preservation treatment is 0-4 ℃, and the preservation treatment is carried out by wrapping with a preservative film.

Preferably, in the step 2, in the drying and dewatering process, the vacuum degree is set to be-0.08 to-0.1 MPa, the temperature is set to be 65 to 85 ℃, and the drying and dewatering time is 3 to 5 hours.

Preferably, in the step 3, the grain diameter of the lactuca sativa powder is 1-5 μm.

Preferably, in the step 4, the mass fraction of the ethanol solution is 60-80%; the mass ratio of the lettuce powder to the ethanol solution is 1: 20-50.

Preferably, in the step 4, the lactuca sativa powder and the ethanol solution are mixed, stirred at the speed of 200-400 rpm for 0.5-1 h at room temperature, subjected to centrifugal separation, and subjected to repeated extraction of the obtained filter residue for three times, and then the supernatant and the precipitate are respectively collected to obtain the first extracting solution and the first filter residue.

Preferably, in the step 5, the alkali solution is a sodium hydroxide solution or a potassium hydroxide solution with a concentration of 1-3 mol/L, and the acid solution is a hydrochloric acid solution with a concentration of 1-3 mol/L; the mass ratio of the first filter residue to the alkali liquor is 1: 30-50.

Preferably, in the step 5, after the first filter residue is mixed with the alkali liquor, the temperature is raised to 35-50 ℃ under the protection of inert gas, and after heat preservation treatment is performed for 3-6 hours, acid liquor is added dropwise to adjust the pH value to be less than 2.

Preferably, in the step 5, ethyl acetate is used as an extracting agent for extraction, and ethanol is used as a solvent for redissolution; and the mass of the ethanol solvent added in the redissolution is 10-20 times of that of the first filter residue.

Preferably, in the step 6, the temperature of the reduced pressure evaporation is set to be 55-65 ℃.

Preferably, the preparation process of the freckle-removing whitening cream comprises the following steps:

s1, weighing water, an emollient, an emulsifier, a humectant and a thickener according to parts by weight, mixing the materials in a first container, heating to 70-80 ℃, and uniformly mixing and dispersing to obtain a first mixed dispersion liquid;

s2, weighing an antioxidant, an astringent, an essence, a preservative, a coloring agent, a sunscreen agent, a chelating agent and a pH regulator according to parts by weight, mixing into a second container, heating to 70-80 ℃, and uniformly mixing and dispersing to obtain a second mixed dispersion liquid;

s3, mixing the first mixed dispersion liquid and the second mixed dispersion liquid into an emulsifying device, heating to 70-80 ℃, adding the arginine ferulate and tranexamic acid weighed according to parts by weight, and uniformly mixing and dispersing to obtain a third mixed dispersion liquid;

and S4, after the third mixed dispersion liquid is cooled to 45-50 ℃, sequentially adding the lactuca sativa polyphenol, tranexamic acid, 4-butyl resorcinol, azelaic acid, bisabolol and nicotinamide which are weighed according to the weight parts, uniformly mixing and dispersing, and cooling to room temperature to obtain the freckle-removing whitening cream.

The invention has the beneficial effects that:

1. according to the invention, the lactuca indica polyphenol, tranexamic acid and nicotinamide are used as active ingredients for whitening and removing freckles, and 4-butyl resorcinol, azelaic acid and bisabolol are used as auxiliary ingredients, so that a cream product with good whitening and freckles removing effects is obtained.

2. The tetrabutyl resorcinol in the invention not only has important effect of inhibiting tyrosinase activity, but also realizes the net penetration and whitening activity by the coordination and simultaneous action of three mechanisms in the melanogenesis process: prior to melanin synthesis, tyrosinase synthesis and glycosylation is hindered, preventing tyrosinase from being absorbed by melanosomes. In the process of Melanin cooperation, the inhibitor inhibits the activity of enzyme, acts as a competitive inhibitor of tyrosinase and TRP1 enzyme, and reduces the formation of intermediate (DHICA Melanin) in the process of promoting the generation of chromogenic Melanin. After melanin synthesis, increased tyrosinase degradation, increased inhibition of melanosome transfer to the stratum corneum, and a slight exfoliation effect.

3. The invention adds tranexamic acid and nicotinamide simultaneously, has the effects of resisting oxidation and improving skin microcirculation to provide skin vitality and luster, and uses the lactuca indica polyphenol to form a safe and efficient repair anti-allergy component, so that the skin surface layer is repaired mildly, the cell metabolism is promoted, and the 'light' spot lightening is realized from the skin surface layer; thereby keeping the bright and moist healthy skin color of the skin. Therefore, the whitening and freckle removing effects are achieved by accelerating the fading of freckles.

4. Compared with the conventional products added with kojic acid and arbutin, the cream product prepared by the invention has lower irritation and better whitening effect. And in the conventional case, kojic acid and niacinamide cannot be used simultaneously, because the acidic condition of kojic acid causes niacinamide to generate nicotinic acid, which not only reduces the whitening effect but also has stronger stimulation to the skin. The lettuce polyphenol used in the invention does not worry about the situation, and the combined action of the lettuce polyphenol, tranexamic acid and nicotinamide has more obvious whitening effect through tests.

5. In addition, the lactuca indica polyphenol in the cream not only has whitening effect, but also has protection and repair effect on sunburn and scytitis of skin, reduces irritation of skin care products to skin, and enhances resistance of skin to the outside.

6. According to the invention, a series of extraction operations are carried out by using the roots and stems of the plant lactuca sativa, the finally extracted lactuca sativa polyphenol has a certain whitening auxiliary effect, and the whitening effect is better improved after the lactuca sativa polyphenol is mixed with tranexamic acid and nicotinamide for use. The method is characterized in that rhizomes of the lactuca sativa are cut into blocks, dried and ground, and then alcohol extraction is carried out, so that cells of the lactuca sativa subjected to vacuum drying and grinding are damaged more greatly, and the release of phenols in the lactuca sativa is promoted to a certain extent by the breakage of the cells, so that the subsequent extraction process can be more efficient; and then performing a bidirectional extraction method of alcohol extraction and alkali extraction, wherein the alcohol extraction is to dissolve free phenol and flavonoid products, the alkali extraction is to dissolve bound phenol, and the reasonable utilization of the bidirectional extraction method can promote the dissolution and extraction of most phenols in the lactuca sativa so as to obtain more extracts.

Drawings

The invention is further illustrated by means of the attached drawings, but the embodiments in the drawings do not constitute any limitation to the invention, and for a person skilled in the art, other drawings can be obtained on the basis of the following drawings without inventive effort.

FIG. 1 is a histogram of the L value measurements;

fig. 2 is a histogram of the results of the melanin content assay.

Detailed Description

For the purpose of more clearly illustrating the present invention and more clearly understanding the technical features, objects and advantages of the present invention, the technical solutions of the present invention will now be described in detail below, but are not to be construed as limiting the implementable scope of the present invention.

The dogwood extract, the ginkgo leaf extract, the peony root extract, the crocus sativus extract and the resveratrol are all purchased from Zhejiang Huishong pharmaceutical Co.

Tyrosinase is a very important part in the process of melanin formation, so that various additives are added into many current cosmetics to control the activity of tyrosinase, so as to inhibit the generation of melanin, and further achieve the effects of removing freckles and whitening. However, the commonly used whitening extracts in existing cosmetics are kojic acid and arbutin.

Arbutin is extracted from folium Vaccinii Vitis-idaeae, contains hydroquinone and glucose as main ingredients, and has effects of inhibiting tyrosinase activity, but has strong irritation to skin, and can be used without exposure to sunlight, and if it is not used properly, it will not inhibit melanin, and can cause melanin precipitation.

Kojic acid is mainly used for whitening the skin, and many consumers use cosmetic products containing kojic acid to lighten freckles and other dark spots of the skin. Kojic acid can inhibit activity of various tyrosinases, has no essential element for forming melanin, and has effects of whitening and lightening speckles. However, kojic acid belongs to acids, so that the kojic acid has certain sensitization, and can cause side effects when being used on intolerant skin, particularly on sensitive muscles and can not be used by pregnant women.

The invention is further described below with reference to the following examples.

Example 1

56 parts of water, 4 parts of nicotinamide, 0.5 part of tranexamic acid, 2.1 parts of lactuca sativa polyphenol, 2.4 parts of 4-butylresorcinol, 0.6 part of azelaic acid, 0.3 part of bisabolol, 12 parts of an emollient, 6 parts of an emulsifier, 8 parts of a humectant, 1.2 parts of a thickener, 0.8 part of an antioxidant, 0.3 part of an astringent, 2.4 parts of a skin conditioner, 0.03 part of an essence, 0.4 part of a preservative, 0.003 part of a colorant, 0.32 part of a sunscreen agent, 0.4 part of a pH regulator and 0.03 part of a chelating agent.

The emollient is isononyl isononanoate, the emulsifier is glyceryl stearate, the humectant is propylene glycol, the thickener is acrylic acid/C10-30 alkanol acrylate cross-linked polymer, the antioxidant is tocopherol acetate, the skin conditioner is dogwood extract, the preservative is methylparaben, and the astringent is allantoin; the sun-screening agent is ethylhexyl methoxycinnamate; the pH regulator is triethanolamine; the chelating agent is disodium EDTA.

The Lactuca sativa polyphenol is extracted from the rhizome of Lactuca sativa.

The method for extracting the lettuce polyphenol comprises the following steps:

step 1, picking fresh Lactuca indica, removing roots and stems of the Lactuca indica, cleaning the surfaces of the roots, draining, and cutting into pieces with a volume of 10mm³Obtaining a lettuce block, and wrapping the lettuce block with a preservative film at the temperature of 0-4 ℃ for later use;

step 2, placing the lettuce blocks in a vacuum drying oven, setting the vacuum degree to be-0.1 MPa, setting the temperature to be 75 ℃, and drying and dewatering for 4 hours to obtain dried lettuce;

step 3, smashing the lactuca sativa into powder, and treating the powder in a superfine pulverizer to obtain lactuca sativa powder with the particle size of 1-5 microns;

step 4, placing the lactuca indica linn powder into an ethanol solution with the mass fraction of 70% for extraction treatment, wherein the mass ratio of the lactuca indica linn powder to the ethanol solution is 1:35, stirring at the speed of 300rpm at room temperature for 0.5h, performing centrifugal separation, repeatedly extracting the obtained filter residue for three times, and collecting supernatant and precipitate respectively to obtain a first extracting solution and first filter residue;

step 5, placing the first filter residue in a 2mol/L sodium hydroxide solution for treatment, wherein the mass ratio of the first filter residue to the sodium hydroxide solution is 1:40, heating to 45 ℃ under the protection of inert gas, carrying out heat preservation treatment for 5 hours, then dropwise adding a hydrochloric acid solution with the concentration of 2mol/L to adjust the pH value to be less than 2, then carrying out centrifugation to collect supernatant, carrying out extraction treatment by ethyl acetate, then adding ethanol 15 times of the mass of the first filter residue, and dissolving again to obtain a second extracting solution;

and 6, combining the first extracting solution and the second extracting solution, and evaporating at 60 ℃ under reduced pressure until the mixture is completely dried to obtain the lactuca indica polyphenol.

The preparation process of the freckle-removing whitening cream comprises the following steps:

s1, weighing water, an emollient, an emulsifier, a humectant and a thickener according to parts by weight, mixing the materials in a first container, heating to 75 ℃, and uniformly mixing and dispersing to obtain a first mixed dispersion liquid;

s2, weighing an antioxidant, an astringent, an essence, a preservative, a coloring agent, a sun-screening agent, a chelating agent and a pH regulator according to parts by weight, mixing into a second container, heating to 75 ℃, and uniformly mixing and dispersing to obtain a second mixed dispersion liquid;

s3, mixing the first mixed dispersion liquid and the second mixed dispersion liquid into an emulsifying device, heating to 75 ℃, adding the arginine ferulate and tranexamic acid weighed according to parts by weight, and uniformly mixing and dispersing to obtain a third mixed dispersion liquid;

and S4, after the third mixed dispersion liquid is cooled to 45 ℃, sequentially adding the lactuca indica polyphenol, tranexamic acid, 4-butyl resorcinol, azelaic acid, bisabolol and nicotinamide which are weighed according to parts by weight, uniformly mixing and dispersing, and cooling to room temperature to obtain the freckle-removing whitening cream.

Example 2

50 parts of water, 3 parts of nicotinamide, 0.1 part of tranexamic acid, 0.5 part of lactuca sativa polyphenol, 1 part of 4-butyl resorcinol, 0.1 part of azelaic acid, 0.1 part of bisabolol, 8 parts of emollient, 5 parts of emulsifier, 6 parts of humectant, 0.5 part of thickener, 0.5 part of antioxidant, 0.1 part of astringent, 0.1 part of skin conditioner, 0.01 part of essence, 0.1 part of preservative, 0.002 part of colorant, 0.2 part of sunscreen agent, 0.1 part of pH regulator and 0.01 part of chelating agent.

The emollient is ethylhexyl palmitate, the emulsifier is potassium cetyl phosphate, the humectant is betaine, the thickener is acrylate/C10-30 alkanol acrylate cross-linked polymer, the antioxidant is sodium metabisulfite, the skin conditioner is ginkgo biloba extract, the preservative is propyl hydroxybenzoate, and the astringent is allantoin; the sun-screening agent is ethylhexyl methoxycinnamate; the pH regulator is triethanolamine; the chelating agent is disodium EDTA.

The Lactuca sativa polyphenol is extracted from the rhizome of Lactuca sativa.

The method for extracting the lettuce polyphenol comprises the following steps:

step 1, picking fresh Lactuca indica, removing leaves and roots of the Lactuca indicaCleaning the surface of the root, draining, and cutting into pieces with volume of 8mm³Obtaining a lettuce block, and wrapping the lettuce block with a preservative film at the temperature of 0-4 ℃ for later use;

step 2, placing the lettuce blocks in a vacuum drying oven, setting the vacuum degree to be-0.08 MPa, setting the temperature to be 65 ℃, and drying and dewatering for 3 hours to obtain dried lettuce;

step 4, placing the lactuca indica linn powder into an ethanol solution with the mass fraction of 60% for extraction treatment, wherein the mass ratio of the lactuca indica linn powder to the ethanol solution is 1:20, stirring at the speed of 200rpm at room temperature for 0.5h, performing centrifugal separation, repeatedly extracting the obtained filter residue for three times, and collecting supernatant and precipitate respectively to obtain a first extracting solution and first filter residue;

step 5, placing the first filter residue in a 1mol/L potassium hydroxide solution for treatment, wherein the mass ratio of the first filter residue to the potassium hydroxide solution is 1:30, heating to 35 ℃ under the protection of inert gas, carrying out heat preservation treatment for 3 hours, then dropwise adding a hydrochloric acid solution with the concentration of 1mol/L to adjust the pH value to be less than 2, then carrying out centrifugation to collect supernatant, carrying out extraction treatment by ethyl acetate, then adding ethanol with the mass being 10 times that of the first filter residue, and dissolving again to obtain a second extracting solution;

and 6, combining the first extracting solution and the second extracting solution, and evaporating at 55 ℃ under reduced pressure until the mixture is completely dried to obtain the lactuca indica polyphenol.

s2, weighing an antioxidant, an astringent, an essence, a preservative, a coloring agent, a sun-screening agent, a chelating agent and a pH regulator according to parts by weight, mixing into a second container, heating to 70 ℃, and uniformly mixing and dispersing to obtain a second mixed dispersion liquid;

s3, mixing the first mixed dispersion liquid and the second mixed dispersion liquid into an emulsifying device, heating to 70 ℃, adding the arginine ferulate and tranexamic acid weighed according to parts by weight, and uniformly mixing and dispersing to obtain a third mixed dispersion liquid;

Example 3

65 parts of water, 6 parts of nicotinamide, 1 part of tranexamic acid, 3 parts of lactuca sativa polyphenol, 3 parts of 4-butylresorcinol, 1 part of azelaic acid, 0.5 part of bisabolol, 15 parts of emollient, 8 parts of emulsifier, 10 parts of humectant, 2 parts of thickener, 1 part of antioxidant, 0.5 part of astringent, 5 parts of skin conditioner, 0.05 part of essence, 0.7 part of preservative, 0.005 part of colorant, 0.45 part of sun-screening agent, 0.6 part of pH regulator and 0.05 part of chelating agent.

The skin conditioner is characterized in that the emollient is ethylhexyl stearate, the emulsifier is cetyl alcohol, the humectant is trehalose, the thickener is polyacrylamide, the antioxidant is butylated hydroxytoluene, the skin conditioner is a peony root extract, the preservative is o-cymene-5-alcohol, and the astringent is allantoin; the sun-screening agent is ethylhexyl methoxycinnamate; the pH regulator is triethanolamine; the chelating agent is disodium EDTA.

The Lactuca sativa polyphenol is extracted from the rhizome of Lactuca sativa.

The method for extracting the lettuce polyphenol comprises the following steps:

step 1, picking fresh Lactuca indica, removing roots and stems of the Lactuca indica, cleaning the surfaces of the roots, draining, and cutting into pieces with a volume of 12mm³Obtaining a lettuce block, and wrapping the lettuce block with a preservative film at the temperature of 0-4 ℃ for later use;

step 2, placing the lettuce blocks in a vacuum drying oven, setting the vacuum degree to be-0.1 MPa, setting the temperature to be 85 ℃, and drying and dewatering for 5 hours to obtain dried lettuce;

step 4, placing the lactuca indica linn powder into an ethanol solution with the mass fraction of 80% for extraction treatment, wherein the mass ratio of the lactuca indica linn powder to the ethanol solution is 1:50, stirring at the speed of 400rpm at room temperature for 1h, performing centrifugal separation, repeatedly extracting the obtained filter residue for three times, and then respectively collecting supernatant and precipitate to obtain a first extracting solution and first filter residue;

step 5, placing the first filter residue in a 3mol/L sodium hydroxide solution for treatment, wherein the mass ratio of the first filter residue to the sodium hydroxide solution is 1:50, heating to 50 ℃ under the protection of inert gas, carrying out heat preservation treatment for 6 hours, then dropwise adding a hydrochloric acid solution with the concentration of 3mol/L to adjust the pH value to be less than 2, then carrying out centrifugation to collect supernatant, carrying out extraction treatment by ethyl acetate, then adding ethanol with the mass being 20 times that of the first filter residue, and dissolving again to obtain a second extracting solution;

and 6, combining the first extracting solution and the second extracting solution, and evaporating at 65 ℃ under reduced pressure until the mixture is completely dried to obtain the lactuca indica polyphenol.

s1, weighing water, an emollient, an emulsifier, a humectant and a thickener according to parts by weight, mixing the materials in a first container, heating to 80 ℃, and uniformly mixing and dispersing to obtain a first mixed dispersion liquid;

s2, weighing an antioxidant, an astringent, an essence, a preservative, a coloring agent, a sun-screening agent, a chelating agent and a pH regulator according to parts by weight, mixing into a second container, heating to 80 ℃, and uniformly mixing and dispersing to obtain a second mixed dispersion liquid;

s3, mixing the first mixed dispersion liquid and the second mixed dispersion liquid into an emulsifying device, heating to 80 ℃, adding the arginine ferulate and tranexamic acid weighed according to parts by weight, and uniformly mixing and dispersing to obtain a third mixed dispersion liquid;

and S4, after the third mixed dispersion liquid is cooled to 50 ℃, sequentially adding the lactuca indica polyphenol, tranexamic acid, 4-butyl resorcinol, azelaic acid, bisabolol and nicotinamide which are weighed according to parts by weight, uniformly mixing and dispersing, and cooling to room temperature to obtain the freckle-removing whitening cream.

Example 4

Compared with the embodiment 1, the freckle-removing whitening cream is characterized in that:

the emollient is polydimethylsiloxane, the emulsifier is PEG-100 stearate, the humectant is butanediol, the thickener is C13-14 isoparaffin, the skin conditioner is crocus sativus extract, and the preservative is CI 19140.

Example 5

the emollient is cyclopentadimethylsiloxane, the emulsifier is ceteareth-20, the humectant is 1, 3-propylene glycol, the thickener is laureth-7, the skin conditioner is resveratrol, and the preservative is CI 16255.

Example 6

the emollient is cyclohexasiloxane, the emulsifier is PEG-75 stearate, the humectant is 1, 2-pentanediol, and the thickener is xanthan gum.

Example 7

the emollient is beeswax, and the humectant is 1, 2-hexanediol.

Example 8

the emollient is polydimethylsiloxane cross-linked polymer, and the humectant is ethylhexyl glycerol.

Example 9

the emollient is selected from isononyl isononanoate, ethylhexyl palmitate, ethylhexyl stearate, polydimethylsiloxane, cyclopentadimethylsiloxane, cyclohexasiloxane, beeswax, and polydimethylsiloxane cross-linked polymer.

The emulsifier is any two of glyceryl stearate, potassium cetyl phosphate, cetyl alcohol, PEG-100 stearate, ceteareth-20, and PEG-75 stearate.

The humectant is any two of propylene glycol, betaine, trehalose, butanediol, 1, 3-propylene glycol, 1, 2-pentanediol, 1, 2-hexanediol, and ethylhexylglycerin.

The thickener is any two of acrylic acid/C10-30 alkanol acrylate cross-linked polymer, acrylate/C10-30 alkanol acrylate cross-linked polymer, polyacrylamide, C13-14 isoparaffin, laureth-7 and xanthan gum.

The antioxidant is any two of tocopherol acetate, sodium metabisulfite and butylated hydroxytoluene.

The skin conditioner is any two of Corni fructus extract, folium Ginkgo extract, radix Paeoniae extract, stigma croci Sativi extract, and resveratrol.

The antiseptic is any two of methyl hydroxybenzoate, propyl hydroxybenzoate, o-cymene-5-alcohol, CI19140, and CI 16255.

Example 10

The emulsifier is any three of glyceryl stearate, potassium cetyl phosphate, cetyl alcohol, PEG-100 stearate, ceteareth-20, and PEG-75 stearate.

The humectant is any three of propylene glycol, betaine, trehalose, butanediol, 1, 3-propylene glycol, 1, 2-pentanediol, 1, 2-hexanediol and ethylhexylglycerin.

The thickening agent is any three of acrylic acid/C10-30 alkanol acrylate cross-linked polymer, acrylate/C10-30 alkanol acrylate cross-linked polymer, polyacrylamide, C13-14 isoparaffin, laureth-7 and xanthan gum.

The antioxidant is any three of tocopherol acetate, sodium metabisulfite and butylated hydroxytoluene.

The skin conditioner is any three of Corni fructus extract, folium Ginkgo extract, radix Paeoniae extract, stigma croci Sativi extract, and resveratrol.

The antiseptic is any three of methyl hydroxybenzoate, propyl hydroxybenzoate, o-cymene-5-alcohol, CI19140, and CI 16255.

To illustrate the invention more clearly, the following experiments were carried out in relation to the whitening cream prepared in example 1:

first, test purpose

With 3D melanoderm model

For a testing tool, the whitening cream sample in example 1 is acted on a 3D melanin skin model in a surface administration mode, and the whitening efficacy of the sample to be tested is evaluated through indexes of three dimensions of apparent chromaticity, apparent brightness (L value) and melanin content of the model after administration.

Second, test materials

2.1 test System

The 3D melanoderm model used for this test

2.2 Primary reagents

M-TA culture solution, PBS, sodium hydroxide, DMSO, absolute ethyl alcohol and diethyl ether.

2.3 Main Equipment

CO₂Incubator (Thermo, 150I), super clean bench (Sujing Antai, SW-CJ-1F), UVB lamp tube (Philips), upright microscope (Olympus, BX53), colorimeter (Denmark, DSMII), digital display constant temperature water bath (Changzhou, China, HH-4A), and microplate reader (BioTek, Epoch).

Third, testing method

3.1 test protocol

Table 1 sample whitening efficacy evaluation protocol

3.2 model reception

Preparing 6-well plates according to the whitening efficacy evaluation scheme in table 1, adding 3.7mLM-TA culture solution to each well, transferring the model cultured on the air-liquid surface for the 3 rd Day (TA3), i.e., the Day of model reception (Day0), to the 6-well plates which are marked; 3 models were required for each experimental group and all model-loaded 6-well plates were transferred to a CO2 incubator (37 ℃, 5% CO 2).

3.3 administration and UVB stimulation

From the Day of model acceptance (Day0), the negative control, positive control and sample groups were subjected to UVB irradiation treatment (50 mJ/cm) daily²) The blank control group was not subjected to UVB irradiation, and only the culture solution was changed every day. The positive control group (KA, 500. mu.g/mL) and the sample group were administered twice in a dose of 10. mu.L by surface administration in Day3 and Day5, respectively. After the model was cultured continuously for 7 days (Day7), the sample was collected and assayed.

3.4 apparent chroma

After the model culture was completed, the appearance photograph was taken with a camera. The specific photographing standard operation is as follows:

(1) a camera mode: manual operation; setting photographing parameters: focal length 5.8mm, aperture F/8, aperture F22, shutter speed 1/80s, ISO 1600.

(2) The melanin skin model is placed in the center of the colorimetric card for photographing.

3.5L value test

And detecting the L value of the model after the apparent chromaticity detection is finished. The specific detection operation is as follows: placing the model on a flat and firm frame

On the hard white plane, the horny layer is placed upwards, the detection hole of the color difference meter is vertically aligned with the surface of the model for detection, and each model is

The readings were repeated three times and the average was taken as the L value reading for the individual model. The model after detection was placed in a clean EP tube for melanin content determination.

3.6 Melanin content test

And (5) performing melanin content detection on the model after the L value detection is finished. The detection operation is as follows:

(1) placing the model in 1.5ml LEP tubes, labeling, adding 1ml LPBS buffer solution into each tube, shaking for 3min with a vortex oscillator, centrifuging at 2000r/min for 10min with a low-temperature high-speed centrifuge, and discarding the supernatant;

(2) sequentially adding 200 mu L of distilled water, 500 mu L of absolute ethyl alcohol and 500 mu L of diethyl ether into the EP tube in the step (1), fully and uniformly mixing, standing at room temperature for 20min, centrifuging at 3000r/min for 5min, and removing supernatant;

(3) adding 1mL of 1mol/L NaOH aqueous solution containing 10% DMSO, and heating in a water bath at 80 ℃ for 40 min;

(4) after the end of the incubation period, 200. mu.L of the supernatant was pipetted into corresponding wells of a well-labeled 96-well plate, OD was read at 405nm and two replicates of each model were tested in the 96-well plate.

Fourthly, statistical analysis of results

The results are expressed as Mean ± SD using GraphPadPrism mapping. The comparison between groups was performed by statistical analysis of t-test. All statistical analyses were two-tailed. p <0.05 was considered to have significant differences, and p <0.01 was considered to have very significant differences.

Fifth, test results

5.2L value test results

The model after the end of the apparent chromaticity measurement was subjected to L value measurement, and the results are shown in table 2 and fig. 1 (histogram of L value measurement results).

And (3) grouping the tests:

TABLE 2 summary of model L values

Test grouping	Mean value of	SD	P-value
				BC	68.33	1.33	/
NC	64.88	1.05	0.024#
				PC	69.27	0.55	0.003**
Whitening and freckle-removing cream	72.43	0.5	0.000**

Remarking: when the statistical analysis is carried out by using a t-test method, compared with a BC group, the significance of an NC group is represented by # and P-value <0.05 is represented by #, and P-value <0.01 is represented by # #; the significance of the sample group and PC group was shown as x, P-value <0.05 and P-value <0.01, respectively, compared to the NC group.

Compared with the BC group, the NC group has a significantly decreased L value, and compared with the NC group, the PC group has a significantly increased L value, which indicates that the test conditions are valid.

Compared with an NC group, the sample whitening and freckle removing cream has a remarkable improvement effect on the L value of a melanin model.

5.3 Melanin content test results

The results of the melanin content test on the model after the end of L value test are shown in table 3 and fig. 2 (histogram of the melanin content test results).

TABLE 3 summary of melanin content measurements

Test set	Mean value (μ g/mL)	SD	P-value
				BC	30.660	1.368	/
NC	34.569	0.850	0.014#
				PC	30.458	1.364	0.011*
Whitening removerSpot cream	20.523	0.660	0.000**

Compared with the BC group, the NC group has obviously increased melanin content, and compared with the NC group, the PC group has obviously decreased melanin content, which shows that the test condition is effective.

Compared with an NC group, the sample whitening and freckle removing cream has a remarkable inhibiting effect on the melanin content of a melanin model.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The freckle-removing whitening cream is characterized by comprising the following components in parts by weight:

2. The spot-removing whitening cream according to claim 1, wherein the emollient is at least one of isononyl isononanoate, ethylhexyl palmitate, ethylhexyl stearate, dimethicone, cyclopentadimethicone, cyclohexasiloxane, beeswax, and dimethicone crosspolymer.

3. The whitening cream with freckle removing effect of claim 1, wherein the emulsifier is at least one of glyceryl stearate, potassium cetyl phosphate, cetyl alcohol, PEG-100 stearate, ceteareth-20 and PEG-75 stearate.

4. The spot-removing whitening cream according to claim 1, wherein the humectant is at least one of propylene glycol, betaine, trehalose, butylene glycol, 1, 3-propanediol, 1, 2-pentanediol, 1, 2-hexanediol, and ethylhexyl glycerin.

5. The spot-removing whitening cream according to claim 1, wherein the thickener is at least one of acrylic acid/C10-30 alkanol acrylate cross-linked polymer, acrylate/C10-30 alkanol acrylate cross-linked polymer, polyacrylamide, C13-14 isoparaffin, laureth-7, and xanthan gum.

6. The whitening cream with freckle removing type according to claim 1, wherein the antioxidant is at least one of tocopherol acetate, sodium metabisulfite and butylated hydroxytoluene.

7. The spot-removing whitening cream according to claim 1, wherein the skin conditioner is at least one of dogwood extract, ginkgo biloba extract, peony root extract, saffron extract and resveratrol.

8. The whitening cream according to claim 1, wherein the antiseptic is at least one of methyl hydroxybenzoate, propyl hydroxybenzoate, o-cymene-5-ol, CI19140 and CI 16255.

9. The spot-removing whitening cream according to claim 1, wherein the astringent is allantoin; the sun-screening agent is ethylhexyl methoxycinnamate; the pH regulator is triethanolamine; the chelating agent is disodium EDTA.

10. The spot-removing whitening cream according to claim 1, wherein the lactuca sativa polyphenol is extracted by the following method: