CN114503986A - Long tube plant aphid repellent and application thereof - Google Patents
Long tube plant aphid repellent and application thereof Download PDFInfo
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- CN114503986A CN114503986A CN202210156407.8A CN202210156407A CN114503986A CN 114503986 A CN114503986 A CN 114503986A CN 202210156407 A CN202210156407 A CN 202210156407A CN 114503986 A CN114503986 A CN 114503986A
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- repellent
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- aphid
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- 241001124076 Aphididae Species 0.000 title claims abstract description 36
- 239000005871 repellent Substances 0.000 title claims abstract description 27
- 230000002940 repellent Effects 0.000 title claims abstract description 27
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical group CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 7
- 241000273311 Aphis spiraecola Species 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 239000004480 active ingredient Substances 0.000 claims description 3
- 241001453659 Onychium Species 0.000 claims 2
- 241001505010 Onychostoma Species 0.000 claims 2
- 235000007516 Chrysanthemum Nutrition 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 12
- 241000721621 Myzus persicae Species 0.000 abstract description 7
- 238000011161 development Methods 0.000 abstract description 6
- 150000001875 compounds Chemical class 0.000 abstract description 5
- 241000526125 Diaphorina citri Species 0.000 abstract description 4
- 230000002265 prevention Effects 0.000 abstract description 4
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 244000189548 Chrysanthemum x morifolium Species 0.000 abstract 1
- 241000721623 Myzus Species 0.000 description 19
- 241000723353 Chrysanthemum Species 0.000 description 17
- KXSDPILWMGFJMM-GUBZILKMSA-N (1s,4s,5r)-4-methyl-1-propan-2-ylbicyclo[3.1.0]hexan-4-ol Chemical compound C([C@@]1(O)C)C[C@]2(C(C)C)[C@H]1C2 KXSDPILWMGFJMM-GUBZILKMSA-N 0.000 description 10
- 230000004634 feeding behavior Effects 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- IHPKGUQCSIINRJ-CSKARUKUSA-N (E)-beta-ocimene Chemical compound CC(C)=CC\C=C(/C)C=C IHPKGUQCSIINRJ-CSKARUKUSA-N 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- DTGKSKDOIYIVQL-QXFUBDJGSA-N (-)-borneol Chemical compound C1C[C@]2(C)[C@H](O)C[C@H]1C2(C)C DTGKSKDOIYIVQL-QXFUBDJGSA-N 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- IHPKGUQCSIINRJ-UHFFFAOYSA-N β-ocimene Natural products CC(C)=CCC=C(C)C=C IHPKGUQCSIINRJ-UHFFFAOYSA-N 0.000 description 3
- 229930006703 (-)-borneol Natural products 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000501828 Acidocella Species 0.000 description 1
- 241000252335 Acipenser Species 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 235000007866 Chamaemelum nobile Nutrition 0.000 description 1
- 241000972195 Chrysanthemum chlorotic mottle viroid Species 0.000 description 1
- 241001648782 Chrysanthemum stem necrosis virus Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 241001508399 Elaeagnus Species 0.000 description 1
- 241001468872 Fumagospora capnodioides Species 0.000 description 1
- 241000735332 Gerbera Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000042664 Matricaria chamomilla Species 0.000 description 1
- 235000007232 Matricaria chamomilla Nutrition 0.000 description 1
- 241001314546 Microtis <orchid> Species 0.000 description 1
- 241000277334 Oncorhynchus Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 244000064622 Physalis edulis Species 0.000 description 1
- KXSDPILWMGFJMM-UHFFFAOYSA-N Sabinene hydrate Chemical compound CC1(O)CCC2(C(C)C)C1C2 KXSDPILWMGFJMM-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 238000009227 behaviour therapy Methods 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- DTGKSKDOIYIVQL-UHFFFAOYSA-N dl-isoborneol Natural products C1CC2(C)C(O)CC1C2(C)C DTGKSKDOIYIVQL-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- JQOAQUXIUNVRQW-UHFFFAOYSA-N hexane Chemical compound CCCCCC.CCCCCC JQOAQUXIUNVRQW-UHFFFAOYSA-N 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000034267 olfactory behavior Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a diaphorina citri repellent and application thereof. The active component of the repellent is cis-4-thujaplicin which is a nontoxic compound and can stop the elongate tubular aphid from eating, pull out the piercing-sucking mouthpart and escape from the eating object. The repellent provided by the invention has an obvious repellent effect on the elongate myzus persicae, can realize a good prevention and control effect, and promotes the sustainable development of the chrysanthemum industry.
Description
Technical Field
The invention belongs to the technical field of pest control, and particularly relates to a diaphorina citri repellent and application thereof.
Background
Long pipe aphid (Macrosiponiella sanborni) is an oligotrophic insect mainly harmful to the plants of the Compositae family, the common hosts being most wild species and cultivars of the Chrysanthemum genus. The myzus microti bur absorbs plant juice, leaves are faded, yellow, wilted and even dried, the growth and development of the chrysanthemum is abnormal, the flowering phase is more harmful, and the quality of the ornamental chrysanthemum and the medicinal chrysanthemum is seriously influenced. The honeydew secreted by the myzus persicae can cause sooty mould and also seriously affect the ornamental value. In addition, the myzus elongatus as a transmission medium of pathogens such as chrysanthemum stem necrosis virus, chrysanthemum chlorotic mottle virus, chrysanthemum dwarfing virus and the like has a very serious threat to the production of cultivated chrysanthemum, and the development of the chrysanthemum industry is severely restricted.
At present, the measures for controlling the myzus citrinopileatus are mainly chemical control by using insecticides, and the killing mode has short-term effect taking speed and lower cost. However, in the long run, a series of problems of pesticide residue exceeding standard, environmental pollution, aphid resistance improvement and the like caused by chemical prevention and control seriously affect the quality safety and ecological environment safety of chrysanthemum products, and greatly restrict the sustainable development of chrysanthemum industry. Therefore, the research and development of the novel aphid repellent have important significance for the control of the elongate tubular aphid of chrysanthemum, and the sustainable development of the chrysanthemum industry is promoted.
Disclosure of Invention
The invention aims to provide a diaphorina citri repellent and application thereof.
In order to achieve the purpose, in a first aspect, the invention provides a diaphorina citri repellent, and the active ingredient is cis-4-thujaplicin.
Preferably, the solvent used to dissolve the active ingredient is n-hexane.
Preferably, the mass percent of the cis-4-thujaplicin is 0.1% -10%.
In a second aspect, the invention provides the use of the repellent in the control (repellency) of aphids.
Furthermore, the control object is the myzus citrinopileatus, in particular to the myzus longipes and adult myzus citrinopileatus.
In a third aspect, the present invention provides any one of the following uses of cis-4-arborol:
1) for the control (repellency) of aphids;
2) is used for preparing aphid repellent.
Preferably, the aphids are the myzus citrinopileatus, in particular the myzus longipes and adult myzus citrinopileatus.
In the present invention, cis-4-thujaitol is a functional compound that exerts a repellent activity, and n-hexane is used as a solvent. Preferably, Cis-4-thujanol (Cis-4-thujanol) has a purity of 99% or more, and n-Hexane (Hexane) has a purity of 95% or more.
By the technical scheme, the invention at least has the following advantages and beneficial effects:
the long pipe aphid repellent for the long pipe aphids of chrysanthemum can effectively make up the blank of the related fields and promote the green prevention and control of the long pipe aphids of chrysanthemum.
And secondly, the components of the repellent provided by the invention are all commercialized products, so that the repellent is convenient to use and easy to popularize.
And thirdly, the compound cis-4-thujaplicin for generating a repellent effect on the physaloma citricola is a non-toxic compound, and can stop feeding the physaloma citricola, pull out the piercing-sucking mouthpart and escape from a feeding object.
And (IV) the repellent provided by the invention has an obvious repellent effect on the elongate myzus persicae, can realize a good prevention and control effect, and promotes the sustainable development of the chrysanthemum industry.
Drawings
FIG. 1 is a graph showing the interference of direct release of 0.1% cis-4-thujaplicin on the feeding behavior of the plant Myzus sieboldii in a preferred embodiment of the present invention.
FIG. 2 is a diagram illustrating the interference of directly releasing 1% alpha-lupene on the feeding behavior of the myzus Julianensis in the preferred embodiment of the present invention.
In the figure, each panel is an image of aphid feeding recorded at 4-second intervals, and "S" is seconds.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.
Example 1 Acidocella aurantiaca behavior selection test on 4 volatile compounds
1. Material method
1.1 feeding and collecting Physalis punctatus
The myzus citrinopsis gerbera is collected from a test greenhouse of national center for flower engineering and technology research of Beijing university of forestry. Before the test, 4-year-old Oncorhynchus deltoides of Aphis citricola is collected and inoculated on an Aphis citricola variety 'Jinglin Kaogong', and the Aphis citricola is sleeved with a gauze bag for feeding. In the Y-tube behavioral selection test, wingless adult aphids were randomly collected from the tulle pouch for the behavioral test.
1.24 preparation of volatile chemical standard
Of the 4 volatile standard products, beta-ocimene (CAS number: 13877-91-3; Sigma product number: W353901) and cis-4-thujaplicin (CAS number: 546-79-2; Sigma product number: 96573) were purchased from Sigma reagent, China; l-borneol (CAS No: 464-45-9; original leaf product No: B30491) and alpha-lupinene (CAS No: 6753-98-6; B29274) were purchased from Shanghai-derived leaf Biotech, Inc. Solvent n-hexane was purchased from Sigma reagent china. Each standard was configured as three gradients of 10%, 1% and 0.1% by mass fraction for use.
1.3 olfactory behavior selection based on Y-type olfactory tester
A custom Y-type glass olfactometer with the diameter of 2cm, the length of a main arm of 10cm and the length of a support arm of 8cm is used for observing behavior selection of the myzus chazus. The main arm is connected with an air sampling instrument vacuum pump, and clean air which is filtered by bamboo charcoal and humidified by distilled water is brought into the two glass sample bottles and then respectively enters the support arms of the Y-shaped olfactometer. The flow rate of air in the olfactometer was controlled at 0.1L/min by a flow meter. A cut 5mm by 40mm sterile filter paper strip is placed in the sample injection bottle, and 10 mul of standard solution is loaded each time. Each group tested 30 aphids and 3 replicates were set. 10 minutes after the start of aeration, the number of aphids 2cm above the arm was counted as the effective selection number. The non-selected ones are removed from the selection.
Experimental data statistical analysis of the significance of differences was performed with SPSS 20.0.
2. Results of the experiment
As can be seen from Table 1, three concentrations of cis-4-thujaplicin showed very significant repelling effect on Acipenser virgatus. The 0.1 percent of alpha-lupinene and the 0.1 percent of beta-ocimene show obvious attraction effect on the elongate chamomile aphids. But when the concentration of alpha-lupinene was increased to 1% and 10%, it appeared to be a significant repellent against myzus Julianus. The increase in β -ocimene concentration has no significant effect. The L-borneol with three concentrations has no obvious influence on the behavior selection of the myzus persicae.
In conclusion, 0.1-10% of cis-4-thujaplicin and 1-10% of alpha-lupinene can generate obvious repellent effect on the elongate pipe aphid. The effect of cis-4-thujaplicin is better, and the repellent effect under the lowest concentration can reach an extremely obvious level.
TABLE 1 Elaeagnus sieboldii selection preference for 4 volatile chemical standards
Note: the numbers of aphids in the tables are the mean ± standard deviation of the three groups of biological replicates, "-" indicates that the sample and control groups differed significantly at a level p <0.05 and "-" indicates that the differences were very significant at a level p < 0.05.
Example 2 interference of cis-4-arborol and alpha-lupinene on the feeding behavior of the plant Myzus sieboldii
According to the results of example 1, 0.1% cis-4-thujanol and 1% alpha-lupinene both significantly repelled adult Aphis citricola. This example further demonstrates the interfering effect of these two compounds on the actual feeding status of myzus julianae.
1. Material method
When the chrysanthemum 'Jinglingqiong' cutting seedling grows to 8-10 leaves, 5 small-tube aphids of chrysanthemum are inoculated to form aphids, and the aphids are bagged to be freely propagated. After 10 days, a certain amount of aphids are proliferated on the inoculated chrysanthemum and are in a normal feeding state. At this time, 10. mu.l of solvent n-hexane (as a control), 0.1% cis-4-thujanol and 1% alpha-lupinene solution were separately pipetted using 10. mu.l pipette gun and released around the aphid feeding site of the experimental plant by 2-3 cm. The behavioral response of aphids was recorded using a video camera.
2. Results of the experiment
This part results in a video format, where one frame of the image presentation is taken every 6 seconds for ease of presentation (fig. 1, 2). As can be seen from 6-26 seconds shown in figure 1 and 6-26 seconds shown in figure 2, after the n-hexane standard substance is directly released, the myzus punctatus is not interfered and still keeps normal feeding behavior. As can be seen from 30-58 seconds in FIG. 1, after 10. mu.l of 0.1% cis-4-thujaplicin was released, the normal feeding behavior of most of the Myzus chazus persicae was disturbed. The method is characterized in that firstly, the piercing-sucking mouthparts are pulled out, then, the piercing-sucking mouthparts climb around, and both adult aphids and aphid have obvious reaction on cis-4-thujanol. However, as seen from 30-58 seconds in FIG. 2, after 10. mu.l of 1% alpha-lupinene is released, the myzus citrinopileatus has no obvious reaction and still keeps a normal feeding state.
In conclusion, the experimental results show that 0.1% cis-4-thujaplicin can obviously interfere with the normal feeding behavior of the myzus persicae and can simultaneously act on the myzus persicae and adult myzus persicae.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (9)
1. The elongate tubular aphid repellent is characterized in that the active component is cis-4-thujaplicin.
2. The repellent according to claim 1, wherein the solvent for dissolving the active ingredient is n-hexane.
3. The repellent according to claim 1 or 2, characterized in that the mass percentage of cis-4-thujaplicin is 0.1% -10%.
4. Use of a repellent according to any one of claims 1 to 3 for controlling aphids.
5. The use as claimed in claim 4, wherein the control object is elongate channel aphid.
6. The use according to claim 5, wherein the control object is the Onychium species, Onychium species.
7. Any one of the following uses of cis-4-thujaplicin:
1) used for preventing and treating aphids;
2) is used for preparing aphid repellent.
8. The use according to claim 7, characterized in that said aphid is the plant Aphis citricola.
9. The use according to claim 8, characterized in that said aphids are the Onychostoma species, the Onychostoma species.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210156407.8A CN114503986A (en) | 2022-02-21 | 2022-02-21 | Long tube plant aphid repellent and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210156407.8A CN114503986A (en) | 2022-02-21 | 2022-02-21 | Long tube plant aphid repellent and application thereof |
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| CN114503986A true CN114503986A (en) | 2022-05-17 |
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| CN202210156407.8A Pending CN114503986A (en) | 2022-02-21 | 2022-02-21 | Long tube plant aphid repellent and application thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1152402A (en) * | 1996-09-24 | 1997-06-25 | 王政国 | Pesticide for cypress and poplar plants |
| US20140242199A1 (en) * | 2011-10-04 | 2014-08-28 | 0903608 B.C. Ltd. | Pest control formulations and methods of making and using same |
| CN108289442A (en) * | 2015-11-26 | 2018-07-17 | 理研香料控股株式会社 | Insects repellant and use its pest repelling method |
| CN113229276A (en) * | 2021-04-30 | 2021-08-10 | 杭州茶菊科技有限公司 | Repellent for myzus elongatus and using method thereof |
-
2022
- 2022-02-21 CN CN202210156407.8A patent/CN114503986A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1152402A (en) * | 1996-09-24 | 1997-06-25 | 王政国 | Pesticide for cypress and poplar plants |
| US20140242199A1 (en) * | 2011-10-04 | 2014-08-28 | 0903608 B.C. Ltd. | Pest control formulations and methods of making and using same |
| CN108289442A (en) * | 2015-11-26 | 2018-07-17 | 理研香料控股株式会社 | Insects repellant and use its pest repelling method |
| CN113229276A (en) * | 2021-04-30 | 2021-08-10 | 杭州茶菊科技有限公司 | Repellent for myzus elongatus and using method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 吴桂苹 等: "云南怒江草果的微波无溶剂萃取及其挥发性风味物质的GC-TOF-MS分析", 《中国调味品》, vol. 45, no. 1, 31 January 2020 (2020-01-31), pages 172 - 179 * |
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