CN114586784B - Apis punctata repellent and application thereof - Google Patents
Apis punctata repellent and application thereof Download PDFInfo
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- CN114586784B CN114586784B CN202210179332.5A CN202210179332A CN114586784B CN 114586784 B CN114586784 B CN 114586784B CN 202210179332 A CN202210179332 A CN 202210179332A CN 114586784 B CN114586784 B CN 114586784B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M29/00—Scaring or repelling devices, e.g. bird-scaring apparatus
- A01M29/12—Scaring or repelling devices, e.g. bird-scaring apparatus using odoriferous substances, e.g. aromas, pheromones or chemical agents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a Apis dorsalis repellent and application thereof. The Apis cerana repellent comprises the following components in percentage by volume: 0.01 to 0.1 percent of decanal, 0.01 to 0.1 percent of heptanal, 0.1 to 1 percent of nonanal, 0.1 to 1 percent of linalool and 97.8 to 99.78 percent of solvent. The repellent for the Apis cerana provided by the invention takes decanal, heptanal, nonanal and linalool as repellent active ingredients, all the active ingredients complement each other, the synergistic effect is achieved, the repellent effect for the Apis cerana is good, the repellent rate of the Apis cerana is as high as 95.43% +/-0.48%, and the repellent rate of the Apis cerana is obviously higher than that of a single active ingredient. The active ingredients are common natural plant ingredients, are harmless to human bodies, biodegradable, environment-friendly and convenient and safe to use.
Description
Technical Field
The invention relates to the technical field of pest control, in particular to a Apis punctata repellent and application thereof.
Background
Apis lucorum (Riptortus pedestris) is an economic pest distributed over soybeans in japan, korea and china. Also known as Apis cerana and Apis spinosa, belongs to the family of Hemiptera, araliaceae, and is used for sucking more than 30 crops such as soybean, kidney bean, sesame, rice, apple, and sweet persimmon. The phenomena of pod shedding, pod incapacitation, kernel malformation and the like caused by the damage of the vigorous clusters of adults and nymphs, which further cause the damage of crop yield and quality, are considered as important factors for the phenomenon of 'green disease' of soybean in Huanghuai sea basin.
At present, the prevention and treatment method of the Apis cerana is mainly chemical prevention and treatment, but the Apis cerana has resistance to various pesticides due to long-term and large-scale use of chemical pesticides, and the chemical prevention and treatment effect is obviously reduced, so that the utilization of a biological prevention and treatment method is urgent. The research report on the effect of the point bee stinkbug repellent is not very common, so the invention provides a novel point bee stinkbug repellent, and has important guiding significance for green and efficient control of the point bee stinkbug.
Disclosure of Invention
The first object of the present invention is to provide a plant-derived pest repellent which is excellent in oviposition and antifeedant repellent effects against Apis cerana. The plant-derived pest repellent comprises decanal, heptanal, nonanal, linalool and a solvent.
Preferably, the plant-derived pest repellent comprises the following components in percentage by volume: 0.01 to 0.1 percent of decanal, 0.01 to 0.1 percent of heptanal, 0.1 to 1 percent of nonanal, 0.1 to 1 percent of linalool and 97.8 to 99.78 percent of solvent.
Preferably, the plant-derived pest repellent comprises the following components in percentage by volume: 0.05 to 0.1 percent of decanal, 0.06 to 0.1 percent of heptanal, 0.6 to 1 percent of nonanal, 0.6 to 1 percent of linalool and 97.8 to 98.69 percent of solvent.
Preferably, the plant-derived pest repellent comprises the following components in percentage by volume: 0.05% decanal, 0.06% heptanal, 0.6% nonanal, 0.6% linalool and 98.69% solvent
Preferably, the plant-derived pest repellent comprises the following components in percentage by volume: 0.05% of decanal, 0.1% of heptanal, 1% of nonanal, 1% of linalool and 97.85% of solvent.
Preferably, the plant-derived pest repellent comprises the following components in percentage by volume: 0.1% decanal, 0.1% heptanal, 1% nonanal, 1% linalool and 97.80% solvent.
Preferably, the solvent is n-hexane or water.
Preferably, the solvent is water.
Further, the insect is Apis punctatus.
A second object of the present invention is to provide the use of the plant-derived pest repellent described above for repelling Apis cerana.
The third object of the present invention is to provide a method for preparing the above plant-derived insect pest repellent, comprising the steps of: adding decanal, heptanal, nonanal and linalool into solvent, stirring and dissolving.
The fourth object of the present invention is to provide the method for repelling a point bee stink bug, which comprises the following steps: the plant-derived pest repellent is sprayed on a place where the prevention of the Apis cerana is required.
The invention has the beneficial effects that:
apis spinosa is used as one of the causes of soybean 'Zhengqing', chemical control is mainly used at the present stage, but long-term chemical control can cause Apis spinosa to generate certain drug resistance, so that the development of an effective and environment-friendly control technical room is key to controlling the pest hazard. The plant-source insect repellent provided by the invention takes decanal, heptanal, nonanal and linalool as repellent active ingredients, all the active ingredients complement each other, and the plant-source insect repellent has a synergistic effect, has good oviposition and antifeedant repellent effects on the Apis, and has a repellent rate of up to 95.43% +/-0.48% on the Apis, and is remarkably higher than that of a single active ingredient. The active ingredients are common natural plant ingredients, are harmless to human bodies, biodegradable, environment-friendly and convenient and safe to use.
Drawings
Figure 1 shows the repellent effect of decanal, heptanal, nonanal, linalool on male and female stinkbug at different concentrations.
FIG. 2 shows the electrophysiological response of various concentrations of decanal, heptanal, nonanal, linalool to Apis androsaceus, apis estrus.
Detailed Description
The following examples are further illustrative of the invention and are not intended to be limiting thereof.
Example 1: y-tube behavioral response
The experiment was performed at room temperature of 24.+ -. 1 ℃ and air relative humidity of 40.+ -. 5% under natural light environment. The Y-type olfactometer (Y-type tube) was washed with acetone and then n-hexane before the experiment. The method comprises the steps of preparing decanal, heptanal, nonanal and linalool into samples to be tested with concentration gradients of 0.1 mug/mu L, 1 mug/mu L and 10 mug/mu L by using normal hexane, taking the normal hexane as a reference, respectively dripping 10 mu L of samples to be tested and the normal hexane on two different filter papers, respectively putting the two filter papers into odor source device bottles on two side walls of a Y-shaped tube, opening an atmosphere sampler, enabling the airflow velocity in the Y-shaped tube to be 100mL/min, putting female and male adult stinkbug with the point bee stinkbug into a release tube, observing for 5min, and considering that the odor source is selected when one arm of the point stinkbug is selected for the first time and the duration is longer than 30 s. Each test insect was used only 1 time, each treated adult had the same sex and at least 30 heads, and each 5 heads were cleaned and arm changed (the two arms of the odor source and control were changed) to eliminate errors due to the asymmetry or orientation of the two arms of the Y-tube.
The results of the behavioral response of the Y-tube are shown in FIG. 1. As can be seen from FIG. 1A, 0.1. Mu.g/. Mu.L, 1. Mu.g/. Mu.L and 10. Mu.g/. Mu.L of decanal had a significant repellent effect on males (P < 0.05), while females were not significant repellent at the three concentrations. As can be seen from FIG. 1B, 0.1. Mu.g/. Mu.L of heptaldehyde has a remarkable repellent effect (P < 0.05) on males, the repellent effect on males is not obvious for the other two concentrations, and the repellent effect on three concentrations is not remarkable for females. As can be seen from FIG. 1C, 1. Mu.g/. Mu.L of nonanal has a remarkable repellent effect (P < 0.05) on males, the repellent effect on males is not obvious for the other two concentrations, and the repellent effect on three concentrations is not remarkable for females. As can be seen from FIG. 1D, linalool at 1. Mu.g/. Mu.L and 10. Mu.g/. Mu.L had a significant repellent effect on males (P < 0.05), linalool at 10. Mu.g/. Mu.L had a significant repellent effect on females (P < 0.05), and the repellent effect on females was not evident at the other two concentrations.
Example 2: EAG relative response value
Electrophysiological responses of male and female adults of Apis Cera chinensis to various concentrations of decanal, heptanal, nonanal and linalool were determined using EAG (Syntech, germany). The specific operation steps are as follows: decanal, heptanal, nonanal and linalool were prepared with n-hexane into samples to be tested having concentration gradients of 1. Mu.g/. Mu.L, 10. Mu.g/. Mu.L and 100. Mu.g/. Mu.L 3, and 10. Mu.L of each sample to be tested or n-hexane was dropped onto the filter paper with n-hexane as a control, and then the filter paper was put into a sample tube. The antenna of the adult Apis dorsalis was cut from the base and the antenna ends were cut a small portion (about 1 mm) and the antenna ends were each glued to the fork electrodes using conductive glue. The continuous air flow at the antenna was adjusted to 5mL/s. When in testing, the air continuous airflow is switched into the stimulating airflow of the sample to be tested, namely, the air continuous airflow is suspended, the stimulating airflow of the switched sample to be tested is 1mL/s, and the duration of the stimulating airflow of the sample to be tested is 0.5s. The resulting electrophysiological signals were recorded with Syntech software. The same dose of decanal, heptanal, nonanal and linalool was stimulated 1 time on 1 antenna, 10 antennas each, and repeated three times.
EAG results are shown in FIG. 2, and the results show that the male adult of Apostichopus japonicus has the highest electrophysiological response value to 100 mug of decanal, heptanal and linalool, and has the highest electrophysiological response value to 10 mug of nonanal; the female adult plant of Apolygus lucorum has the highest electrophysiological response value to 100 μg of decanal, heptanal, nonanal and linalool.
Example 3: field experiment
Test site: the planting area of the soybean field in the experimental field of the Fuyang national academy of sciences of Fuyang of Anhui province is 40 mu, and the pest damage rate of Apis cerana is more than 60%.
The test method comprises the following steps: with random block design, 1m per cell 2 A total of 87 cells, wherein 3 cells are blank control cells, 3 cells are sprayed with water as control cells, and different concentrations are sprayedAnd 81 repellent cells. The number of Apostium melittosum on 20 soybean plants in 87 cells was investigated before spraying the repellent and recorded. Decanal, heptanal, nonanal, linalool and water were formulated according to the volume percentages of table 1 to obtain 100mL of repellent solution, which was sprayed on different cells, 3 replicates per group. The number of insect population was investigated three days after spraying and recorded. Repellent ratio = number of insect population after repellent is sprayed/number of insect population before repellent is sprayed x 100%.
TABLE 1
From the results in table 1, the best combinations of repellent point stinkbug effect were 0.05% decanal, 0.06% heptanal, 0.6% nonanal, 0.6% linalool, 100% water make up, and 95.43% ± 0.48% repellent rate. The second combination is that the volume percentage is 0.05 percent of decanal, 0.1 percent of heptanal, 1 percent of nonanal, 1 percent of linalool and 100 percent of water, and the repellent rate is 89.92 percent plus or minus 0.93 percent; the volume percentage of the combination is 0.1 percent of decanal, 0.1 percent of heptanal, 1 percent of nonanal, 1 percent of linalool and 100 percent of water, and the repellent rate is 89.24 percent plus or minus 0.59 percent.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that the above-mentioned preferred embodiment should not be construed as limiting the invention, and the scope of the invention should be defined by the appended claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and such modifications and adaptations are intended to be comprehended within the scope of the invention.
Claims (8)
1. The plant-source insect pest repellent is characterized by comprising the following components in percentage by volume: 0.01 to 0.1 percent of decanal, 0.01 to 0.1 percent of heptanal, 0.1 to 1 percent of nonanal, 0.1 to 1 percent of linalool and 97.8 to 99.78 percent of solvent; the pests are Apis ceratus.
2. The plant-derived pest repellent according to claim 1, characterized by comprising the following components in percentage by volume: 0.05 to 0.1 percent of decanal, 0.06 to 0.1 percent of heptanal, 0.6 to 1 percent of nonanal, 0.6 to 1 percent of linalool and 97.8 to 98.69 percent of solvent.
3. The plant-derived pest repellent according to claim 2, characterized by comprising the following components in percentage by volume: 0.05% decanal, 0.06% heptanal, 0.6% nonanal, 0.6% linalool and 98.69% solvent.
4. A plant-derived pest repellent as claimed in any one of claims 1 to 3, wherein the solvent is n-hexane or water.
5. The plant-derived pest repellent according to claim 4, wherein the solvent is water.
6. Use of a plant-derived pest repellent according to any one of claims 1 to 3 for repelling Apis cerana.
7. A method of preparing the plant-derived pest repellent of any one of claims 1 to 3, comprising the steps of: adding decanal, heptanal, nonanal and linalool into solvent, stirring and dissolving.
8. The method for repelling the Apis cerana, which is characterized by comprising the following steps of: a plant-derived pest repellent according to any one of claims 1 to 3 for spraying onto a locus where prevention of a lygus lucorum is desired.
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| CN115136958B (en) * | 2022-07-27 | 2023-02-28 | 华中农业大学 | Plant-source attractant for wheat moths and method for preventing and controlling wheat moths |
| CN115968877A (en) * | 2022-12-09 | 2023-04-18 | 河南农业大学 | Slug repellent and preparation method thereof |
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