CN108094428B - Application of citrus canker pathogen-generated micromolecules as thiazole bactericide synergist in disease control - Google Patents
Application of citrus canker pathogen-generated micromolecules as thiazole bactericide synergist in disease control Download PDFInfo
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- CN108094428B CN108094428B CN201711152617.5A CN201711152617A CN108094428B CN 108094428 B CN108094428 B CN 108094428B CN 201711152617 A CN201711152617 A CN 201711152617A CN 108094428 B CN108094428 B CN 108094428B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
Abstract
The invention discloses a citrus cankerXanthomonas axonopodispv.citri（Xac) The novel application of the generated small molecular compound (DSF) as a synergist of thiazole bactericides comprises the following specific steps: the bactericidal effect of the thiazole bactericide can be obviously improved by adding a small molecular compound with a certain concentration into the mainstream thiazole bactericide (such as zinc thiazole and the like) in the market at present. The DSF is derived from citrus cankerXacThe fermentation liquor is extracted (or can be artificially and chemically synthesized), and the extraction and separation process is simple. The synergist can obviously improve the sterilization effect of thiazoles, greatly reduce the use amount of chemical pesticides, weaken the drug resistance of pathogenic bacteria and finally promote the harmonious development of the planting industry and the environment.
Description
Technical Field
The invention relates to the field of plant protection by biological control technology, in particular to citrus cankerXanthomonas axonopodispv.citri（Xac) The generated micromolecule compound is used as a green and safe novel synergist to improve the bactericidal effect of thiazole bactericides such as zinc thiazole and reduce the application amount of chemical pesticides in disease control.
Background
In the comprehensive prevention and control of plant diseases, chemical pesticide control is widely favored due to the characteristics of quick response, remarkable effect and the like, and China is one of the most serious countries in the world abusing chemical agents. With the concern and importance of people on personal health, food safety and ecological environment, the reduction of chemical agents, especially the dosage of traditional high-toxicity and high-residue chemical pesticides, is imperative in agricultural production. Research and develop and popularize novel pesticides with low toxicity, low residue, good environmental compatibility and remarkable prevention and treatment effect; the research and development of novel pesticide synergist can increase pesticide effect and reduce pesticide consumption, and has urgent and bright prospect. The traditional synergist improves the pesticide effect of chemical pesticides by improving the wetting, spreading, dispersing, detention, permeability and the like of the pesticides, but has the defects of difficult synthesis, environmental pollution and the like. Starting from the perspective of pathogenic bacteria, the bactericide effect of the pesticide is improved by changing the life habits of pathogenic microorganisms and inhibiting and weakening the drug resistance of pathogenic bacteria, the usage amount of the pesticide is reduced, the cost is reduced, and the protection of the ecological environment is the development direction of a novel synergist in the future, so that the pesticide synergist not only can prolong the life cycle of pesticide varieties, but also is popular due to the green safety characteristic of the pesticide varieties.
The earlier stage research of the invention finds the pathogen of citrus cankerXacTo produce novel small molecules with different structures, for example Wang et al (Wang L, He Y, Gao Y,et al.. A bacterial cell-cell communicationsignal with cross-kingdom structural analogues[J]molecular Microbiology,2004,51(3):903 one 912.), Ellen et al (Beaulieu E D, Ionescu M, Chatterjee S, et al]Mbio, 2013, 4(1): 00539-12) disclose DSF,XfThe structure of the DSF is as follows:
however, no report on the aspect of improving the bactericidal effect of the medicament by researching the small molecular compound exists at present.
Disclosure of Invention
Our research found that oranges can be found whether indoors or in the fieldThe orange ulcer disease preventing and treating medicine zinc thiazole is added with low-concentration pathogenic bacteriaXacThe generated small molecular compound can remarkably improve the sterilization effect of the medicament, thereby reducing the using amount of the medicament and the reagent. The small molecule compound refers to DSF and/orXfDSF。
The use of the small molecular compound as a synergist and a pesticide in the prevention and treatment of plant diseases is not reported.
The invention aims to provide a new application of a small molecule compound.
The purpose of the invention is realized by the following technical scheme:
provides the application of the small molecular compound as a synergist and thiazole bactericide in preventing and treating citrus canker.
The small molecule compound is derived from citrus canker pathogenic bacteriaXanthomonas axonopodispv.citriExtracting in fermentation culture solution or artificially chemically synthesizing.
The extraction method of the small molecular compound is to collect the citrus canker pathogen by centrifugationXacThe supernatant of the overnight culture broth was extracted with ethyl acetate, separated and purified by high performance liquid chromatography (HP L C), and the active substance was collected and confirmed to be structuralXacThe DSF produced, and derivatives thereofXfDSF。
Mixing the separated and extracted small molecular compounds with different concentrations with thiazole bactericide (zinc thiazole) to treat pathogenic bacteriaXacCounting the growth of colony, comparing the mixed small molecules with different concentrations with thiazole bactericide to treat indoor and potted orange pathogenic bacteriaXacThe sterilization effect of (1). Test results show that the small molecular compound with a certain concentration can obviously improve the effect of thiazole bactericide (zinc thiazole) on pathogenic bacteriaXacThe bactericidal effect of the composition is developed into a novel efficient bactericidal synergist which has bright prospect in preventing and treating plant diseases.
Based on the control of the later production and application cost, the invention provides that the small molecular compound and the thiazole bactericide are mixed to prepare a mixture with the concentration range of 0.01 mu M-1 mM for preventing and treating citrus canker, and more preferably 10-500 mu M.
Compared with the prior art, the invention has the following beneficial effects:
the research on the mixed use of the micromolecular compound as the synergist and the thiazole bactericide can obviously improve the pesticide effect of the bactericide, reduce the use amount of chemical agents, reduce the cost and have important significance for solving the increasingly prominent resistance problem of pathogenic bacteria to the bactericide.
1) The invention starts from pathogenic bacteria, can obviously improve the drug effect by using natural unsaturated fatty acid micromolecule compounds generated by bacteria and mixing thiazole bactericide (zinc thiazole) by starting from the pathogenic bacteria, provides a new thought for researching and developing a novel green and environment-friendly sterilization synergist by reducing the dosage of the bactericide, and has important significance for solving the increasingly prominent drug resistance problem of the pathogenic bacteria.
2) The technical scheme that the citrus canker pathogen micromolecule DSF is used as a synergist for preventing and controlling citrus bacterial plant diseases is determined.
Drawings
FIG. 1 shows a schematic view of aXfSterilization and synergism of DSF mixed zinc thiazole on citrus canker (bacterial liquid treatment diluent 10)-6Multiple), wherein, note: 1A: WT +5% CH3OH; 1B: zn (treated with zinc thiazole alone); 1C: 500 times and 60 mu M of zinc thiazoleXfMixing DSF; 1D: 500 times and 80 mu M of zinc thiazoleXfMixing DSF; 1E: 500 times and 100 mu M of zinc thiazoleXfAnd (5) DSF blending.
FIG. 2XfThe statistical result of the bactericidal and synergistic effects of DSF mixed zinc thiazole on citrus canker is shown as follows: zn represents the bacterial liquid treated with 500 times of zinc thiazole alone (as a positive control); zn-20 to Zn-100 expressed as 20 μ M to 100 μ MXfDSF is mixed with thiazole zinc treatment bacterial liquid; signals of different concentrationsXfThe synergistic ratio of DSF as synergist to mixed zinc thiazole = CFU/(zinc thiazole +) singly used for zinc thiazoleXfDSF synergist) CFU
FIG. 3 shows that the sterilization and synergism of zinc thiazole blended with DSF on citrus canker (bacterial liquid treating diluent 10)-6Multiple). Wherein, note: 3A: WT +5% CH3OH; 3B: zn (treated with zinc thiazole alone); 3C: 500 times and 300 mu M of zinc thiazoleMixing DSF; 3D: mixing 500 times of zinc thiazole with 400 mu M DSF; 3E: 500 times of zinc thiazole and 500 mu M DSF.
FIG. 4 is a statistical result of the bactericidal and synergistic effects of DSF mixed zinc thiazole on citrus canker. Wherein, note: zn represents the bacterial liquid treated with 500 times of zinc thiazole alone (as a positive control); Zn-50-Zn-500 represents that 50 mu M-500 mu MDSF is selected to mix with zinc thiazole to treat bacterial liquid; the synergistic ratio of different concentration signals DSF as a synergist to be mixed with zinc thiazole = CFU for zinc thiazole alone/(zinc thiazole + DSF synergist) CFU.
FIG. 5XfAnd (3) counting the potted plant inoculation result of the sterilization synergistic effect of DSF mixed with zinc thiazole on the citrus canker. Wherein, note: zn represents that 500 times of solution of zinc thiazole is independently used for treating the young leaves of the potted plant inoculated with the pathogenic bacteria; zn-60 to Zn-80 are selected in the range of 60 to 80 μ MXfDSF is mixed with 500 times of thiazole zinc solution to treat tender leaves of plants inoculated with pathogenic bacteria; small molecules of different concentrationsXfThe synergistic ratio of DSF as synergist to mixed zinc thiazole = thiazole zinc single use/(zinc thiazole +)XfDSF synergist).
FIG. 6 shows statistical results of potted plant inoculation of bactericidal synergistic effect of DSF mixed zinc thiazole on citrus canker. Note: zn represents that 500 times of solution of zinc thiazole is independently used for treating the young leaves of the potted plant inoculated with the pathogenic bacteria; Zn-300-Zn-500 shows that 500 times of DSF mixed with thiazole zinc solution with concentration of 300 mu M-500 mu M is selected to treat tender leaves of plants inoculated with pathogenic bacteria; the synergistic ratio of the different concentrations of small molecule DSF as the synergist to be mixed with the thiazole zinc = thiazole zinc singly/(thiazole zinc + DSF synergist).
FIG. 7 DSF orXfAnd (3) the result of statistics of the sterilization and synergism of DSF mixed zinc thiazole on pathogenic bacteria of rice basal rot is obtained. Wherein: note: FIG. 7A shows a control with 0.5% methanol added to the medium; 7B represents that the bacterial liquid is treated by 500 times of solution of zinc thiazole alone; 7C 20. mu.MXfDSF is mixed with thiazole zinc treatment bacterial liquid; FIG. 7D shows the addition of a 0.5% methanol control to the medium; 7E represents that the bacterial liquid is treated by 500 times of solution of zinc thiazole alone; the 7F shows that 300 μ M DSF mixed zinc thiazole treatment bacterial liquid is selected.
Detailed Description
The invention is further illustrated by the following detailed description of specific embodiments, which are not intended to be limiting but are merely exemplary.
The concentration of the mother liquor of 30% zinc thiazole adopted in the examples 1 to 3 is 10mg/m L, the working concentration is 500 times of the diluent, the test concentration of the small molecular compound is 100 mM..
Example 4 the concentration of mother liquor of 30% zinc thiazole adopted is 10mg/m L, the working concentration is 500 times of diluent, the test concentration of micromolecules is 100 mM. the example of the invention shows that micromolecule compounds and thiazole chemical reagents in a certain concentration range are used as biological source synergists to treat rice basal saprophytic bacteriaEC1The composition has certain synergistic bactericidal effect, and the combination of partial thiazole bactericides and small molecular compounds as the synergist can achieve the synergistic effect.
Example 1 DSF andXfpreparation of DSF
Will be cultured overnightXacInoculating seed solution into YEB culture medium, culturing at 28 deg.C and 200rpm for 24 hr, centrifuging, and collecting Citrus canker germXacAnd (4) supernatant of the culture solution. The obtained supernatant is used for extracting small molecules.
The invention adopts the conventional method for separating and extracting compounds to separate and extract active substances in fermentation liquor (see two prior art documents mentioned in the background of the specification). Centrifugally collecting citrus canker germsXacAdding ethyl acetate with the same volume as the supernatant into the culture supernatant, standing at 4 deg.C for 2 hr, treating at 20 deg.C for 1 hr with shaking table, extracting with separating funnel, collecting the upper layer, suspending the collected upper layer with rotary evaporator, evaporating the organic solvent, eluting with 2-3m L methanol, evaporating the methanol solution containing active ingredients, concentrating, and further purifying, analyzing, and collecting the activity by high performance liquid chromatography (HP L C)A substance. Performing structural identification on the active ingredient, and confirming that the active ingredient is DSF and derivatives thereof respectivelyXfDSF。
EXAMPLE 2 blending of Small molecule Compounds with 30% Zinc Thiazolate against LeptosphaeriaXacThe sterilization and synergism of
The extract is dissolved by using methanol as a solvent, the extract is mixed with a zinc thiazole reagent of 10mg/m L to treat the citrus canker germ solution with a certain OD value, and the total bacterial colony Count (CFU) in unit volume is used for measuring the sterilization and synergism of the extract with different concentrations on the zinc thiazole.
Adopting a statistical CFU method: preparing a small molecular compound into a test reagent with a certain concentration by using methanol as a solvent, accurately adding a certain amount of small molecular compound solution, mixing the small molecular compound solution with 500 times of the zinc thiazole reagent, and culturing the mixture overnight until the OD is reached600=1.0XacIn the bacterial liquid, micromolecular compounds with different concentrations, 500-fold zinc thiazole reagent and the bacterial liquid are simultaneously treated, the bacterial liquid treated by 500-fold zinc thiazole is independently used as a positive control, the bacterial liquid is cultured at 28 ℃ and 200rpm for 24 hours, the treated bacterial liquid is diluted in a gradient manner, 200 mu L bacterial liquid treatment liquid is used for coating a culture plate (the culture medium is L B), each concentration is poured into a culture plate with 3 times of repetition, after a plurality of days in an incubator at 28 ℃, the colony count of the plate is combined with figure 1, and the CFU of 1A untreated control CK is 4 × 1091B CFU of 2.5 × 10 treated with Zinc thiazole alone9500 times and 60 mu M of 1C zinc thiazoleXfThe CFU of the DSF compounding treatment is 4 × 108 1D Zinc thiazole 500 times and 80 mu MXfThe CFU of the DSF compounding treatment is 1.6 × 108 1E Zinc thiazole 500 times and 100 mu MXfThe CFU of the DSF compounding treatment is 1.2 × 108. In conjunction with fig. 2, it follows: zn-20 is expressed as 20. mu.MXfThe ratio of the sterilization and the synergy of the DSF to the zinc thiazole is 1.3 times, and the Zn-40 is expressed as 40 mu MXfThe sterilization synergistic ratio of DSF to zinc thiazole is 6 times, Zn-60 is expressed as 60 mu MXfThe ratio of the sterilization and the synergy of the DSF to the zinc thiazole is 6.5 times, and the Zn-80 is expressed as 80 mu MXfThe ratio of the sterilization and the synergy of the DSF to the zinc thiazole is 16 times, and the Zn-100 is expressed as 100 mu MXfThe ratio of the sterilization synergistic effect of the DSF to the zinc thiazole is 21 times, and the colony count of a flat plate is calculated to obtain that the CFU of the control CK without the treatment of 3A is 4 × 1093B sheetCFU of 2.5 × 10 treated with zinc thiazole alone9The CFU of the 3C zinc thiazole mixed with 300 mu M DSF is 7.5 × 108The CFU of the 3D zinc thiazole mixed with 400 mu M DSF is 108The CFU of the 1E zinc thiazole prepared by mixing 500 times with 500 mu M DSF is 5 × 107。
In conjunction with fig. 4, it follows: zn-50 is expressed as 1.2 times of the sterilization synergistic ratio of 50 MuM DSF to zinc thiazole, Zn-100 is expressed as 1.1 times of the sterilization synergistic ratio of 100 MuM DSF to zinc thiazole, Zn-200 is expressed as 1.1 times of the sterilization synergistic ratio of 200 MuM DSF to zinc thiazole, Zn-300 is expressed as 2 times of the sterilization synergistic ratio of 300 MuM DSF to zinc thiazole, Zn-400 is expressed as 16 times of the sterilization synergistic ratio of 400 MuM DSF to zinc thiazole, and Zn-500 is expressed as 30 times of the sterilization synergistic ratio of 500 MuM DSF to zinc thiazole.
Example 3 greenhouse potting test for preventing and controlling citrus canker pathogen by mixing small molecular compound and zinc thiazole as synergist
Citrus canker (A)Xanthomonas axonopodispv.Citri) Preparing suspension of bacteria, streaking on L B medium for 2 days, inoculating single colony into L B liquid medium, and culturing OD overnight in 28 deg.C thermostat600=0.5, ready for inoculation.
The method comprises selecting four-season orange young leaves with similar size, color and growth as experimental inoculation object, sterilizing the young leaves with 75% alcohol, puncturing the leaves from the back with syringe needle, puncturing 8 perforations at two sides of the leaf far axis, and inoculating 5 μ L OD above the perforations600And (4) inoculating the bacterial liquid suspension of which the concentration is 0.5, and then culturing the bacterial liquid suspension under the conditions of room temperature and natural illumination, and performing conventional water and fertilizer management.
The tender leaves of the Chinese holly after being inoculated with the pathogenic bacteria for 3 days are respectively sprayed with a test reagent prepared by mixing 500 times of thiazole zinc with micromolecule DSF with different concentrations and prepared by tap water, and a treatment reagent taking 500 times of thiazole zinc as a positive control, L B culture medium is used as a negative control, each tender leaf is sprayed with 1m L of the reagent, and each treatment is repeated for 3 times.
Mixing zinc thiazole with small molecular compound solutions with different concentrations to treat citrus fruit leaves inoculated with pathogenic bacteria for three days, sampling, cutting the area with disease spots of each treated leaf into a sterilized mortar, adding a small amount of quartz sand and 2M L L B culture medium, grinding the mixture into a homogenate, placing the homogenate in a 5M L centrifuge tube, adding 3M L L B culture medium, shaking the mixture evenly, filtering the mixture by using sterilized gauze, suspending the obtained filtrate for 10000g/2 min and 1M L culture medium, repeating the suspending twice, suspending by using 200 mu L L B culture medium, diluting the different concentration gradients of 200 mu L coated plates, observing the growth density of pathogenic bacteria colonies, counting, and combining the results of a graph 5 that Zn-60 is represented by using 60 mu M of small molecular compound solutionXfThe ratio of the sterilization synergy of DSF to zinc thiazole is 2 times, Zn-80 is expressed as 80 mu MXfThe ratio of the sterilization and the synergy of the DSF to the zinc thiazole is 8 times, and the Zn-100 is expressed as 100 mu MXfThe sterilization synergistic ratio of DSF to zinc thiazole is 25 times. Combining the results of FIG. 6: zn-300 shows that the ratio of the sterilization synergy of 300 MuM DSF to zinc thiazole is 2 times, Zn-400 shows that the ratio of the sterilization synergy of 400 MuM DSF to zinc thiazole is 5 times, and Zn-500 shows that the ratio of the sterilization synergy of 500 MuM DSF to zinc thiazole is 35 times.
Example 4 DSF orXfDSF and thiazole zinc are mixed to be used as synergist for treating pathogenic bacteria of rice basal rotEC1The sterilization and synergism of
The extract is dissolved by using methanol as a solvent, the extract is mixed with 10mg/m L thiazole zinc to treat the bacterial liquid of the pathogenic bacteria of the rice basal rot with a certain OD value, and the bactericidal synergistic effect of the extract with different concentrations on the thiazole zinc is measured by using the total bacterial Community (CFU) in unit volume.
Adopting a statistical CFU method: preparing a small molecular compound into a test reagent with a certain concentration by using methanol as a solvent, accurately adding a certain amount of small molecular compound solution, mixing the small molecular compound solution with 500 times of the zinc thiazole reagent, and culturing the mixture overnight until the OD is reached600=1.0EC1In the bacterial liquid, the working concentration range of micromolecular DSF in the bacterial liquid is between 0.01 mu M and 50 mu M, the compounds DSF with different concentrations, 500-fold zinc thiazole and the bacterial liquid are simultaneously treated, the bacterial liquid treated by 500-fold zinc thiazole is independently used as a positive control, and the bacterial liquid is placed in a 28-fold containeroC. Culturing at 200rpm for 24 hoursThereafter, the treated cell suspension was subjected to gradient dilution and plated with 200. mu. L cell suspension treatment (L B medium), 3 replicates of each concentration of the culture plate, and after several days in an incubator at 28 ℃ C., the results of the experiment were observed and counted, and as shown in FIG. 7, the CFU of the control CK without treatment of 7A was 7.5 × 1077B treatment with Zinc thiazole aloneEC1CFU of 6 × 1075, 500 times and 20 mu M of 7C zinc thiazoleXfThe CFU of the DSF compounding treatment is 2.5 × 1077D untreated control CK had a CFU of 7.5 × 1077E treatment with Zinc thiazole aloneEC1CFU of 6 × 107The CFU of the 7F zinc thiazole mixed with 100 mu M DSF is 5 × 105。
Claims (8)
1. Pathogenic bacteria of citrus cankerXanthomonas axonopodispv.citri（Xac) The generated small molecular compound is used as a biological synergist of zinc thiazole to have a synergistic control effect on citrus canker or rice basal rot, and the small molecular compound refers to DSF and/orXfDSF, and the concentration of the small molecule compound when mixed with zinc thiazole: the concentration of the DSF is 400. mu.M-1 mM, and the concentration of the DSF isXfThe DSF is 60 μ M to 1 mM.
2. Use according to claim 1, characterized in that: the preparation method of the micromolecule DSF comprises the following steps:
(1) inoculating the seed liquid of the overnight cultured bacteria into a YEB culture medium, and performing shaking culture;
(2) collecting the culture supernatant by centrifugation;
(3) adding ethyl acetate with the same volume as the supernatant, standing, and treating in a normal temperature shaking table;
(4) extracting with a separating funnel, and collecting upper layer liquid;
(5) suspending and evaporating the collected upper layer of separated liquid by using a rotary evaporator, evaporating the organic solvent to dryness, and eluting by using methanol;
(6) the methanol solution containing the active ingredient is evaporated to dryness and concentrated, and then the active substance is further purified, analyzed and collected.
3. The use according to claim 2, wherein the small molecule compound is prepared by culturing at 200rpm at 28 ℃ for 24 hours in step (1).
4. The use according to claim 2, wherein the standing of step (3) of the preparation method of the small molecule compound is standing at 4 ℃.
5. The use according to claim 2, wherein the shaking table treatment time in step (3) of the preparation method of the small molecule compound is 2 h.
6. The use according to any one of claims 1 to 5, wherein the zinc thiazole bactericide is 30% zinc thiazole, the mother liquor concentration is 10mg/m L, and the working concentration is 500 times of the dilution.
7. The bactericide mixed preparation is characterized in that zinc thiazole is used as an active ingredient, and pathogenic bacteria of citrus canker are further addedXanthomonas axonopodispv.citri（Xac) The produced small molecule compound is used as synergist, and the small molecule DSF compound refers to DSF and/orXfDSF, at a concentration of: the concentration of the DSF is 400. mu.M-1 mM, and the concentration of the DSF isXfThe DSF is 60 μ M to 1 mM.
8. The compounded formulation of claim 7, wherein the zinc thiazole is 30% zinc thiazole, the mother liquor concentration is 10mg/m L, and the working concentration is 500 times of the dilution.
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Non-Patent Citations (3)
| Title |
|---|
| Diffusible signal factor (DSF) quorum sensing signal and structurally related molecules enhance the antimicrobial efficacy of antibiotics against some bacterial pathogens;Yinyue Deng 等;《BMC Microbiology》;20141231;第1-9页 * |
| 新的群体感应信号分子-DSF;田静等;《中国化学会第七届有机化学学术会议分组邀请报告摘要》;20111112;第71页 * |
| 柑橘溃疡病防控的新型杀菌增效剂研究;邓嘉茹等;《中国植物病理学会2017年学生年会论文集》;20170725;第412页 * |
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