CN114482087A - Method for solidifying side slope by microorganism mineralization filling-magnesia carbonization guniting - Google Patents
Method for solidifying side slope by microorganism mineralization filling-magnesia carbonization guniting Download PDFInfo
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- CN114482087A CN114482087A CN202111607843.4A CN202111607843A CN114482087A CN 114482087 A CN114482087 A CN 114482087A CN 202111607843 A CN202111607843 A CN 202111607843A CN 114482087 A CN114482087 A CN 114482087A
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- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 title claims abstract description 83
- 239000000395 magnesium oxide Substances 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000003763 carbonization Methods 0.000 title claims abstract description 33
- 230000033558 biomineral tissue development Effects 0.000 title claims abstract description 32
- 244000005700 microbiome Species 0.000 title claims abstract description 17
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 claims abstract description 54
- 238000011049 filling Methods 0.000 claims abstract description 47
- 239000004575 stone Substances 0.000 claims abstract description 26
- 230000000813 microbial effect Effects 0.000 claims abstract description 23
- 239000002344 surface layer Substances 0.000 claims abstract description 17
- 239000002689 soil Substances 0.000 claims description 79
- 239000011435 rock Substances 0.000 claims description 39
- 239000000243 solution Substances 0.000 claims description 39
- 239000007788 liquid Substances 0.000 claims description 25
- 238000005507 spraying Methods 0.000 claims description 21
- 230000001580 bacterial effect Effects 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000007711 solidification Methods 0.000 claims description 17
- 230000008023 solidification Effects 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 16
- 239000002002 slurry Substances 0.000 claims description 15
- 230000035699 permeability Effects 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 238000011282 treatment Methods 0.000 claims description 9
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- 241000606860 Pasteurella Species 0.000 claims description 6
- 238000007605 air drying Methods 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000002985 plastic film Substances 0.000 claims description 6
- 229920006255 plastic film Polymers 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 230000010355 oscillation Effects 0.000 claims description 4
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 claims description 3
- 239000007983 Tris buffer Substances 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 229910002091 carbon monoxide Inorganic materials 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 238000005553 drilling Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 3
- 230000008020 evaporation Effects 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 238000003892 spreading Methods 0.000 claims description 3
- 230000007480 spreading Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 238000010276 construction Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000008929 regeneration Effects 0.000 abstract description 2
- 238000011069 regeneration method Methods 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 11
- 239000000203 mixture Substances 0.000 description 10
- 239000004568 cement Substances 0.000 description 8
- 229910000019 calcium carbonate Inorganic materials 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 239000013078 crystal Substances 0.000 description 5
- 239000010410 layer Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000011777 magnesium Substances 0.000 description 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 101000965313 Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) Aconitate hydratase A Proteins 0.000 description 3
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229910021532 Calcite Inorganic materials 0.000 description 2
- 241000193469 Clostridium pasteurianum Species 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 241000193395 Sporosarcina pasteurii Species 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical class OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- -1 carbonic acid compound Chemical class 0.000 description 1
- 238000010000 carbonizing Methods 0.000 description 1
- 239000010438 granite Substances 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D17/00—Excavations; Bordering of excavations; Making embankments
- E02D17/20—Securing of slopes or inclines
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B28/00—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements
- C04B28/02—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements containing hydraulic cements other than calcium sulfates
- C04B28/10—Lime cements or magnesium oxide cements
- C04B28/105—Magnesium oxide or magnesium carbonate cements
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B40/00—Processes, in general, for influencing or modifying the properties of mortars, concrete or artificial stone compositions, e.g. their setting or hardening ability
- C04B40/02—Selection of the hardening environment
- C04B40/0231—Carbon dioxide hardening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D15/00—Handling building or like materials for hydraulic engineering or foundations
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
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- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
- C04B2111/00474—Uses not provided for elsewhere in C04B2111/00
- C04B2111/00732—Uses not provided for elsewhere in C04B2111/00 for soil stabilisation
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- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
- C04B2111/00474—Uses not provided for elsewhere in C04B2111/00
- C04B2111/00767—Uses not provided for elsewhere in C04B2111/00 for waste stabilisation purposes
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- Consolidation Of Soil By Introduction Of Solidifying Substances Into Soil (AREA)
Abstract
The invention discloses a method for solidifying a side slope by microorganism mineralization filling-magnesium oxide carbonization guniting, and belongs to the technical field of geotechnical engineering. The invention mainly solves the problem of curing of internal gaps and small micro-cracks on the surface layer of the high fill side slope of the earth and the stone. The invention adopts a microbial mineralization filling method to cure the gaps inside the high fill side slope of the earth and the stone and adopts a magnesium oxide carbonization guniting method to cure the surface layer of the side slope. Compared with the prior art, the curing agent has good curing effect and relatively good environmental benefit and social benefit. In addition, the method is more suitable for the requirements of environmental protection and modern engineering construction, and is beneficial to resource regeneration, green and clean, and environment-friendly.
Description
Technical Field
The invention belongs to the technical field of geotechnical engineering, and relates to a method for solidifying a side slope by microbial mineralization filling-magnesia carbonization guniting.
Background
The invention is mainly used for curing the high fill side slope of the earth and the stone. Different from a natural side slope or an excavation side slope, the earth-rock mixture high-fill side slope is large in height, large in earth-rock engineering quantity, various in composition and extremely uneven in structure, and in the earth-rock mixture high-fill side slope, the porosity of soil is far larger than that of stones, so that the compressibility of the soil is far larger than that of the stones, and therefore under the action of the seepage force of overlying load and water, the slope is prone to uneven settlement, rectangular lattices on the surface of the slope side slope are separated from the slope body, the effect of slope prevention and control cannot be achieved, and major property loss or serious social influence is caused. Therefore, in order to avoid uneven settlement of the side slope, the inside of the soil body is solidified and gaps are filled by a microorganism mineralization filling method, the porosity of the inside of the high-fill side slope of the soil-stone mixture is reduced, and the microorganism mineralization filling generates adhesive substances to strengthen the adhesive force between the stones and the soil particles, so that the strength of the high-fill side slope and the impermeability strength of the soil body are enhanced; the active magnesium oxide is sprayed on the surface of the slope for carbonization, so that the strength is increased, and the permeability coefficient is reduced. I.e. by both methods to increase the stability of the slope.
The cement grouting method, which is a commonly used grouting method in the existing engineering, has higher strength and durability, but has poor consistency, can not be completely filled into particle gaps of a high fill side slope of an earth-rock mixture, and can not achieve the effect of reducing the compressibility and permeability of the high fill side slope of the earth-rock mixture, so that a microorganism mineralization filling method curing technology and active magnesium oxide surface guniting curing are provided for solving the problems.
A microorganism mineralization filling method solidification technology; by using MICP technology, calcium carbonate precipitation is induced by microorganisms (Bacillus pasteurianus), and the Bacillus pasteurianus catalyzes and decomposes urea in the environment by secreting high-activity urease, so that CO is increased3 2-In a short time, a large amount of calcium carbonate precipitates are generated. Since calcium ions are exchanged with internal substances through bacteria, so that the calcium ions are accumulated in the bacteria, carbonate is combined with the calcium ions on the surfaces of the bacteria, calcium carbonate precipitates are formed on the surfaces of the bacteria, and the microbial mineralization products are mainly calcium carbonate calcite crystals, wherein the bacteria provide crystal nuclei for the mineralized matter crystals. Because the side slope is filled with earth, stone and miscellaneous soil, the pores are more, the strength is lower, and crystal nuclei are generated in the pores of the soil body through MICP biological grouting, so that the purpose of filling the gaps of the earth and the stone is achieved, the porosity of the earth slope is reduced, and the impermeability strength of the side slope soil is enhanced. And the crystals mainly comprising calcium carbonate and calcite generated by MICP biological grouting can be cemented with surrounding soil particles, so that the effect of reinforcing the slope is achieved, and the strength is improved.
Spraying and curing the surface of the active magnesium oxide; and carrying out green material guniting on the surface of the earth and rock high fill slope, for example, the green material such as MgO is used for spraying. Because the active magnesium oxide (MgO) is carbonized and solidifiedThe chemical technology is a new method which is low-carbon, environment-friendly and is used for treating weak soil and is proposed in recent years. The active magnesium oxide (MgO) reacts with water to form Mg (OH)2Their cementing ability is superior to Ca (OH)2But much weaker than cement hydration products such as CSH, but Mg (OH)2With CO2Carbonic acid compounds which react (carbonize) to form magnesium, in contrast to CaCO3The carbonic acid compound of magnesium has strong cementing capability, the accumulated pore volume of the surface filling is reduced by using magnesium oxide (MgO) guniting, and the porosity is continuously reduced along with the increase of the carbonization time, so that the impermeability is greatly enhanced. Compared with the traditional cement guniting, the magnesium oxide (MgO) is more suitable for the requirements of environmental protection and modern engineering construction. The cement mainly shows that the resource consumption is serious, and the CO is high2Discharge and environmental pollution.
Disclosure of Invention
The microbial mineralization filling method is adopted to cure the gaps in the high-fill soil-rock slope, and the magnesia carbonized guniting method is adopted to cure the surface layer of the slope, wherein the former reduces the porosity among particles and enhances the cohesive force among particles from the interior of the high-fill soil-rock slope, so that the compressibility of the high-fill soil-rock slope is reduced, and the latter solves the problem of permeability of the slope from the surface layer of the high-fill soil-rock slope, and the combination of the two effectively solves the problem of uneven settlement of the high-fill soil-rock slope.
The object of the invention is thus achieved. The invention provides a method for solidifying a side slope by microbial mineralization filling-magnesia carbonization guniting, which is characterized in that a microbial mineralization filling method is adopted to solidify gaps inside a high earth and rock filling side slope, and a magnesia carbonization guniting method is adopted to solidify the surface layer of the side slope, and the method comprises the following specific steps:
step 1, preparation of microorganism mineralized filling material and magnesium oxide carbonized spray material
Step 1.1, the microbial mineralized filling material comprises bacterial liquid and cementing liquid, and the bacterial liquid and the cementing liquid are prepared as follows:
the culture solution is prepared by adopting a formula ATCC 1376 NH4-YE recommended by American strain preservation center, and specifically comprises the following components: sterilizing the culture solution at 121-123 ℃ for 20-30 min, placing the culture solution on a super clean workbench for ultraviolet sterilization and ventilation, and cooling the culture solution to room temperature for later use;
placing the culture solution into a container, and adding the pasteurella bacillus into the culture solution by using a sterile pipette to obtain a bacterial solution, wherein the volume ratio of the pasteurella bacillus to the culture solution is 1: 100; then putting the bacterial liquid into an incubator for culture, wherein the temperature of the incubator is set to be 28-32 ℃, the oscillation frequency is set to be 200-320 rpm, and the culture time is 48-72 h;
placing a calcium chloride and urea solution with a molar ratio of 50: 50 in a beaker, adding deionized water, stirring, and dissolving to form a cementing solution, wherein the mass concentration of the calcium chloride and the urea in the cementing solution is 1.0%;
step 1.2, the magnesium oxide carbonization spraying slurry material is magnesium oxide carbonization spraying slurry and is prepared as follows:
taking on-site soil near a soil-rock high-fill side slope site, cleaning surface impurities, spreading and air-drying the on-site soil near the site, crushing the soil after air-drying, and sieving the crushed soil by a sieve of 5-10 mm;
the treated field soil is called field dry soil;
taking active magnesium oxide, drying the active magnesium oxide and sieving the active magnesium oxide by a sieve of 5mm-10 mm;
the treated magnesium oxide is called as magnesium oxide dry powder;
uniformly mixing dry magnesium oxide powder in dry soil on site, and adding water to prepare a magnesium oxide carbonized spraying slurry, wherein the mass parts of the dry magnesium oxide powder, the water and the dry soil on site are (20-25) to (25-30) to 100 in sequence;
step 2, arranging grouting holes in the land of the earth and rock high fill side slope to be solidified, specifically, arranging N rows of grouting holes on the slope surface of the earth and rock high fill side slope in parallel with the slope top, wherein the first row is positioned at the slope top, and the distance between every two rows is 5-7 m; each row comprises M grouting holes, the distance between two adjacent grouting holes is 2-3M, the depth of each grouting hole is 6-10M, and a grouting pipe is embedded into each grouting hole;
step 3, carrying out internal gap solidification by microorganism mineralization filling
The concept of defining one fill is as follows: firstly, 500L-1000L of bacterial liquid is simultaneously injected into the MxN grouting pipes obtained in the step 2, standing is carried out for 4h, then cementing liquid with the same volume as the bacterial liquid is simultaneously injected into the MxN grouting pipes, and standing is carried out for 4 h;
repeatedly filling for 2-5 times every 24-48 h;
recording the soil and stone high filling side slope which is subjected to the microbial mineralization filling as a filled soil and stone high filling side slope;
step 4, detection of internal gap solidification
And (3) drilling and sampling on the filled soil-rock high fill slope seven days after the microbial mineralization filling is finished, marking as a gap solidified soil sample, sealing and storing the gap solidified soil sample in a laboratory, and detecting the permeability coefficient delta of the gap solidified soil sample1：
If delta1Of the order of > 10-7cm/s, returning to the step 3 to carry out microbial mineralization filling again;
if delta1Of order of 10 or less-7cm/s, curing the internal gap to meet the requirement, and entering the step 5;
step 5, magnesium oxide carbonization guniting is carried out for surface layer solidification
In the filled earth and stone high fill side slope field, spraying magnesia carbonized spraying slurry onto the surface soil of the earth and stone high fill side slope by a slurry spraying machine, covering the filled earth and stone high fill side slope field with a black plastic film to prevent water evaporation, inserting a vent pipe into the black plastic film, and carrying out CO (carbon monoxide) treatment through the vent pipe2Gas carbonization is carried out for 24-48 h;
recording the soil-rock high-fill side slope subjected to magnesium oxide carbonization guniting in the step 5 as the soil-rock high-fill side slope subjected to the two treatments;
step 6, detecting surface layer solidification
Taking a proper amount of soil samples from the soil-rock high fill side slope field after the two treatments, recording the soil samples as micro-crack solidified soil samples, sealing and storing the micro-crack solidified soil samples in a laboratory, and detecting the permeability coefficient delta of the micro-crack solidified soil samples2：
If delta2Of the order of > 10-7cm/s, returning to the step 5 to carry out magnesia carbonization guniting again;
if delta2Of order of 10 or less-7cm/s, the surface layer is cured to meet the requirement, and the curing is finished.
Preferably, the culture solution prepared by the American type culture Collection recommended formula ATCC 1376 NH4-YE has the following composition: 20g yeast extract, 10g (NH)4)２SO40.13mol/L Tris Buffer, deionized water to 1L, and 1mol/L HCl to adjust the pH of the culture solution to 9.0.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention adopts the microorganism mineralization filling method to carry out the internal solidification of the soil-rock high-fill side slope, forms the sediment through the bacillus pasteurii, has good solidification effect, and has relatively good environmental benefit and social benefit compared with the cement grouting solidification.
2. The invention adopts a microorganism mineralization filling method, has short curing period, simple process, easy culture of microorganisms and quick propagation.
3. Compared with cement grouting solidification, the microbial mineralization filling method adopted by the invention has the advantages of small consistency, easiness in filling of internal soil particle gaps and reduction of compressibility.
4. Compared with the traditional cement guniting, the invention adopts the magnesia carbonized guniting material, and is more suitable for the requirements of environmental protection and modern engineering construction. The cement mainly shows that the resource consumption is serious, and the CO is high2Discharge and environmental pollution.
5. The invention adopts the magnesium oxide carbonization spraying material, and can consume CO in the carbonization process2Thereby removing carbon and protecting environment.
6. The invention is beneficial to resource regeneration, green and clean and environment-friendly.
Drawings
FIG. 1 is a schematic diagram of a curing position of a high fill side slope of earth and rock according to an embodiment of the invention.
Detailed Description
The present invention will be described in detail below with reference to examples and the accompanying drawings.
FIG. 1 is a schematic diagram of a curing position of a high fill side slope of earth and rock according to an embodiment of the invention. The method for solidifying the side slope by microbial mineralization filling-magnesia carbonization guniting is characterized in that gaps in the high-fill side slope of the earth and rock are solidified by a microbial mineralization filling method, and the surface layer of the side slope is solidified by a magnesia carbonization guniting method, and comprises the following specific steps:
step 1, preparation of microorganism mineralized filling material and magnesium oxide carbonized spray material
Step 1.1, the microbial mineralized filling material comprises bacterial liquid and cementing liquid, and the bacterial liquid and the cementing liquid are prepared as follows:
the culture solution is prepared by adopting a formula ATCC 1376 NH4-YE recommended by American strain preservation center, and specifically comprises the following components: sterilizing the culture solution at 121-123 ℃ for 20-30 min, placing the culture solution on a super clean workbench for ultraviolet sterilization and ventilation, and cooling the culture solution to room temperature for later use;
placing the culture solution into a container, and adding the pasteurella bacillus into the culture solution by using a sterile pipette to obtain a bacterial solution, wherein the volume ratio of the pasteurella bacillus to the culture solution is 1: 100; then putting the bacterial liquid into an incubator for culture, wherein the temperature of the incubator is set to be 28-32 ℃, the oscillation frequency is set to be 200-320 rpm, and the culture time is 48-72 h;
placing the calcium chloride and urea solution with the molar ratio of 50: 50 into a beaker, adding deionized water, stirring, and dissolving to form a cementing solution, wherein the mass concentration of the calcium chloride and the urea in the cementing solution is 1.0%.
In this example, the culture solution prepared according to the American type culture Collection recommended formula ATCC 1376 NH4-YE has the following composition: 20g yeast extract, 10g (NH)4)2SO40.13mol/L Tris Buffer, deionized water to 1L, and 1mol/L HCl to adjust the pH of the culture solution to 9.0.
In this example, the culture broth was sterilized at 121 ℃ for 20 min. The bacterial liquid is put into an incubator for culture, the temperature of the incubator is set to be 30 ℃, the oscillation frequency is set to be 250rpm, and the culture time is 60 hours.
Step 1.2, the magnesium oxide carbonization spraying slurry material is magnesium oxide carbonization spraying slurry and is prepared as follows:
taking on-site soil near a soil-rock high-fill side slope site, cleaning surface impurities, spreading and air-drying the on-site soil near the site, crushing the soil after air-drying, and sieving the crushed soil by a sieve of 5-10 mm;
the treated field soil is called field dry soil;
taking active magnesium oxide, drying the active magnesium oxide and sieving the active magnesium oxide by a sieve of 5mm-10 mm;
the treated magnesium oxide is called as magnesium oxide dry powder;
the dry magnesium oxide powder is evenly mixed in the dry soil on site, and then water is added to prepare the carbonized magnesium oxide spraying slurry, wherein the mass parts of the dry magnesium oxide powder, the water and the dry soil on site are 20-25 percent, 25-30 percent and 100 percent in sequence.
In the embodiment, the mass parts of the magnesium oxide dry powder, the water and the field dry soil are 20 percent to 25 percent to 100 percent in sequence.
Step 2, arranging grouting holes in the land of the earth and rock high fill side slope to be solidified, specifically, arranging N rows of grouting holes on the slope surface of the earth and rock high fill side slope in parallel with the slope top, wherein the first row is positioned at the slope top, and the distance between every two rows is 5-7 m; each row comprises M grouting holes, the distance between two adjacent grouting holes is 2M-3M, the depth of each grouting hole is 6-10M, and a grouting pipe is embedded in each grouting hole.
In the present embodiment, the distance between the grouting holes in each row is 5M, the distance between two adjacent grouting holes in each row is 2M, the depth of the grouting hole is 8M, and N is 5 and M is 6, that is, there are 30 grouting holes in total.
Step 3, carrying out internal gap solidification by microorganism mineralization filling
The concept of defining one-time padding is as follows: 500L-1000L of bacterial liquid is injected into the MXN grouting pipes obtained in the step 2 at the same time, standing is carried out for 4h, then cementing liquid with the same volume as the bacterial liquid is injected into the MXN grouting pipes at the same time, and standing is carried out for 4 h;
the filling is repeated for 2-5 times every 24-48 h.
In this example, the amount of the bacterial suspension injected in one filling was 800L, and the filling was repeated 3 times within 36 hours.
Recording the soil and stone high filling side slope which is subjected to the microbial mineralization filling as a filled soil and stone high filling side slope;
step 4, detection of internal gap solidification
And (3) drilling and sampling on the filled soil-rock high fill slope seven days after the microbial mineralization filling is finished, marking as a gap solidified soil sample, sealing and storing the gap solidified soil sample in a laboratory, and detecting the permeability coefficient delta of the gap solidified soil sample1：
If delta1Of the order of > 10-7cm/s, returning to the step 3 to carry out microbial mineralization filling again;
if delta1Of order of 10 or less-7cm/s, the curing of the internal gap meets the requirement, and the step 5 is carried out.
In the present embodiment, δ1Of order of 10 or less-7cm/s, the curing of the internal gap meets the requirement, and the step 5 is directly carried out.
Step 5, magnesium oxide carbonization guniting is carried out for surface layer solidification
In the filled earth and stone high fill side slope field, spraying magnesia carbonized spraying slurry onto the surface soil of the earth and stone high fill side slope by a slurry spraying machine, covering the filled earth and stone high fill side slope field with a black plastic film to prevent water evaporation, inserting a vent pipe into the black plastic film, and carrying out CO (carbon monoxide) treatment through the vent pipe2Carbonizing for 24-48 h by gas;
and 5, recording the soil-rock high-fill side slope subjected to magnesium oxide carbonization guniting in the step 5 as the soil-rock high-fill side slope after the two times of treatment.
In this example, CO2The gas carbonization time was 36 h.
Step 6, detecting surface layer solidification
Taking a proper amount of soil samples from the soil-rock high fill side slope field after the two treatments, recording the soil samples as micro-crack solidified soil samples, sealing and storing the micro-crack solidified soil samples in a laboratory, and detecting the permeability coefficient delta of the micro-crack solidified soil samples2：
If delta2Of the order of > 10-7cm/s, returning to the step 5 to carry out magnesia carbonization guniting again;
if delta2Of order of 10 or less-7cm/s, the surface layer is cured to meet the requirement, and the curing is finished.
In the present embodiment, δ2Of order of 10 or less-7cm/s, the surface layer is cured to meet the requirement, and the curing is finished.
The site of the embodiment is a fill slope of the soil and rock height of a second-stage project of a seventh resource thermal power plant in Guangzhou city and a peripheral site, the fill slope is mainly located in a hillock depression and a gully section on the south side of a main plant, the west side of a production building and the west side of a slag comprehensive utilization plant, and the fill slope is about 23m at most. The site covering layer comprises, from top to bottom, a fourth artificial filling soil layer (Q/4ml /) miscellaneous filling soil, plain filling soil, a slope accumulated soil layer (Q/4dl /) silty clay, a alluvial soil layer (Q/4al /) silty clay, silt and a residual accumulated soil layer (Q/4el /) sandy cohesive soil; the underburden is Yanshan period (γ) granite. In the construction process of the project, the site landform is combined to excavate and fill around the building structure to form the soil-stone mixture high-fill side slope.
The soil-rock mixture high fill slope on site is solidified by adopting the method, and the permeability coefficient delta of the gap solidified soil sample is detected1And permeability coefficient delta of small micro-crack solidified soil sample2Are all less than or equal to 10-7cm/s, the curing goal is completed.
Claims (2)
1. A method for solidifying a side slope by microbial mineralization filling-magnesia carbonization guniting is characterized in that a microbial mineralization filling method is adopted to solidify gaps inside a high earth and stone filling side slope, and a magnesia carbonization guniting method is adopted to solidify the surface layer of the side slope, and comprises the following specific steps:
step 1, preparation of microorganism mineralized filling material and magnesium oxide carbonized spray material
Step 1.1, the microbial mineralized filling material comprises bacterial liquid and cementing liquid, and the bacterial liquid and the cementing liquid are prepared as follows:
the culture solution is prepared by adopting a formula ATCC 1376 NH4-YE recommended by American strain preservation center, and specifically comprises the following components: sterilizing the culture solution at 121-123 ℃ for 20-30 min, placing the culture solution on a super clean workbench for ultraviolet sterilization and ventilation, and cooling the culture solution to room temperature for later use;
placing the culture solution into a container, and adding the pasteurella bacillus into the culture solution by using a sterile pipette to obtain a bacterial solution, wherein the volume ratio of the pasteurella bacillus to the culture solution is 1: 100; then putting the bacterial liquid into an incubator for culture, wherein the temperature of the incubator is set to be 28-32 ℃, the oscillation frequency is set to be 200-320 rpm, and the culture time is 48-72 h;
placing a calcium chloride and urea solution with a molar ratio of 50: 50 in a beaker, adding deionized water, stirring, and dissolving to form a cementing solution, wherein the mass concentration of the calcium chloride and the urea in the cementing solution is 1.0%;
step 1.2, the magnesium oxide carbonization spraying slurry material is magnesium oxide carbonization spraying slurry and is prepared as follows:
taking on-site soil near a soil-rock high-fill side slope site, cleaning surface impurities, spreading and air-drying the on-site soil near the site, crushing the soil after air-drying, and sieving the crushed soil by a sieve of 5-10 mm;
the treated field soil is called field dry soil;
taking active magnesium oxide, drying the active magnesium oxide and sieving the active magnesium oxide by a sieve of 5mm-10 mm;
the treated magnesium oxide is called as magnesium oxide dry powder;
uniformly mixing dry magnesium oxide powder in dry soil on site, and adding water to prepare a magnesium oxide carbonized spraying slurry, wherein the mass parts of the dry magnesium oxide powder, the water and the dry soil on site are (20-25) to (25-30) to 100 in sequence;
step 2, arranging grouting holes in the land of the earth and rock high fill side slope to be solidified, specifically, arranging N rows of grouting holes on the slope surface of the earth and rock high fill side slope in parallel with the slope top, wherein the first row is positioned at the slope top, and the distance between every two rows is 5-7 m; each row comprises M grouting holes, the distance between two adjacent grouting holes is 2M-3M, the depth of each grouting hole is 6-10M, and a grouting pipe is embedded in each grouting hole;
step 3, carrying out internal gap solidification by microorganism mineralization filling
The concept of defining one fill is as follows: 500L-1000L of bacterial liquid is injected into the MXN grouting pipes obtained in the step 2 at the same time, standing is carried out for 4h, then cementing liquid with the same volume as the bacterial liquid is injected into the MXN grouting pipes at the same time, and standing is carried out for 4 h;
repeatedly filling for 2-5 times every 24-48 h;
recording the soil and stone high fill side slope which is subjected to the microbial mineralization filling as a filled soil and stone high fill side slope;
step 4, detection of internal gap solidification
And (3) drilling and sampling on the filled soil-rock high fill slope seven days after the microbial mineralization filling is finished, marking as a gap solidified soil sample, sealing and storing the gap solidified soil sample in a laboratory, and detecting the permeability coefficient delta of the gap solidified soil sample1：
If delta1Of the order of > 10-7cm/s, returning to the step 3 to carry out microbial mineralization filling again;
if delta1Of order of 10 or less-7cm/s, curing the internal gap to meet the requirement, and entering the step 5;
step 5, magnesium oxide carbonization guniting is carried out for surface layer solidification
In the filled earth and stone high fill side slope field, spraying magnesia carbonized spraying slurry onto the surface soil of the earth and stone high fill side slope by a slurry spraying machine, covering the filled earth and stone high fill side slope field with a black plastic film to prevent water evaporation, inserting a vent pipe into the black plastic film, and carrying out CO (carbon monoxide) treatment through the vent pipe2Gas carbonization is carried out for 24-48 h;
recording the soil-rock high-fill side slope subjected to magnesium oxide carbonization guniting in the step 5 as the soil-rock high-fill side slope subjected to the two treatments;
step 6, detecting surface layer solidification
Taking a proper amount of soil samples from the soil-rock high fill side slope field after the two treatments, recording the soil samples as micro-crack solidified soil samples, sealing and storing the micro-crack solidified soil samples in a laboratoryDetecting the permeability coefficient delta of the solidified soil sample with small microcracks2：
If delta2Of the order of > 10-7cm/s, returning to the step 5 to carry out magnesia carbonization guniting again;
if delta2Of order of 10 or less-7cm/s, the surface layer is cured to meet the requirement, and the curing is finished.
2. The method of claim 1, wherein the culture solution of the american type culture collection recommendation formula ATCC 1376 NH4-YE is composed of: 20g yeast extract, 10g (NH)4)2SO40.13mol/L Tris Buffer, deionized water to 1L, and 1mol/L HCl to adjust the pH of the culture solution to 9.0.
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