CN113123353A - Method for solidifying redbed slope surface soil by microbial preparation - Google Patents
Method for solidifying redbed slope surface soil by microbial preparation Download PDFInfo
- Publication number
- CN113123353A CN113123353A CN202110416256.0A CN202110416256A CN113123353A CN 113123353 A CN113123353 A CN 113123353A CN 202110416256 A CN202110416256 A CN 202110416256A CN 113123353 A CN113123353 A CN 113123353A
- Authority
- CN
- China
- Prior art keywords
- slope
- red layer
- curing
- soil
- solidified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D17/00—Excavations; Bordering of excavations; Making embankments
- E02D17/20—Securing of slopes or inclines
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
Abstract
The invention discloses a method for solidifying surface soil of a red layer slope by using a microbial preparation, and belongs to the technical field of geotechnical engineering. The method comprises the following steps: preparing a bacterial liquid and a cementing liquid; solidifying the small micro cracks of the surface soil of the red layer side slope; solidifying the surface of surface soil of the red layer side slope; comprehensively solidifying the topsoil of the red layer slope. The method adopts an unmanned aerial vehicle spraying mode, is simple and convenient to operate, the cementing liquid and the bacterial liquid can fully enter surface cracks of the red layer side slope, the cementing liquid and the bacterial liquid can fully contact with surface soil of the red layer side slope, a hard and low-permeability hardened layer can be formed on the surface of the surface soil of the red layer side slope, the influence of rainfall on the surface soil of the red layer side slope is reduced, the possibility of geological disasters such as collapse, landslide and the like of the surface of the red layer side slope is reduced, and the method has the characteristics of good curing effect, low cost, simplicity in operation and the like.
Description
Technical Field
The invention belongs to the technical field of geotechnical engineering, and relates to a method for solidifying surface soil of a red layer slope by using a microbial preparation. In particular to a biological curing method for curing a red layer side slope by utilizing bacteria.
Background
The red layer is a middle and new generation detritus sedimentary rock stratum which takes red as a main color tone and continental facies sedimentation as a main color tone, and is widely distributed in the southwest, northwest, China and south China; the lithology is mainly composed of argillaceous rocks and sandstone soft and hard rocks alternately, the hard rocks are relatively hard and high in strength, the soft rocks are relatively soft and have the characteristics of easy softening in water, easy influence of rainfall, weak weather resistance and the like, and the side slopes are slopes with certain slopes on two sides of the roadbed for ensuring stability of the roadbed. The precipitation seeps into the red layer side slope to expand the small micro cracks on the surface of the red layer side slope and form larger cracks, so that the stability of the red layer side slope is influenced. Moreover, the surface soil of the red layer side slope is easily affected by rainfall, so that geological disasters such as landslide and collapse occur, great negative effects are brought to engineering construction, and meanwhile, the safety of lives and properties of people is seriously threatened. Therefore, in order to solve the problem, a method for solidifying topsoil of the red layer slope by using a microbial preparation is provided, which repairs cracks on the topsoil of the red layer slope and forms a hardened layer on the topsoil of the red layer slope, so as to improve the water resistance of the topsoil of the red layer slope.
The used microbial preparation is mainly obtained by a microbial induced calcium carbonate precipitation technology, wherein the microbial induced calcium carbonate precipitation means that calcium carbonate is generated by certain microbes in the metabolism process of the microbes, and loose soil particles and the like are cemented together to improve the engineering property of soil. The microbial preparation is used for curing the cracks on the top layer of the red layer slope and the surface of the top layer soil of the red layer slope, so that the water resistance of the top layer soil of the red layer slope can be obviously improved, the permeability coefficient of the top layer soil of the red layer slope is reduced, the influence of rainfall on the top layer soil of the red layer slope is effectively reduced, the possibility of geological disasters such as landslide and collapse of the top layer soil of the red layer slope is reduced, the smooth progress of engineering construction is guaranteed, and the life and property safety of people is guaranteed.
Disclosure of Invention
The invention aims to provide a method for solidifying surface soil of a red layer side slope by using a microbial preparation, which is used for solving the problems in the construction field of the red layer side slope and has the characteristics of good solidifying effect, low cost, simplicity in operation and the like.
The invention aims to realize the purpose, and provides a method for solidifying topsoil of a red layer slope by using a microbial preparation, which comprises the following steps:
step 1, preparation of bacterial liquid and cementing liquid
Step 1.1, adopting American type culture Collection to recommend a formula ATCC 1376NH4Preparing a culture solution by YE, sterilizing the culture solution at 121-123 ℃ for 20-30 min, placing the culture solution on an ultra-clean workbench for ultraviolet sterilization and ventilation, and cooling the culture solution to room temperature for later use;
step 1.2, after the temperature of the culture solution is reduced to room temperature, taking the culture solution into a container, adding the pasteurella bacillus into the culture solution by using a sterile pipette to obtain a bacterial solution, wherein the volume ratio of the pasteurella bacillus to the culture solution is 1: 100, putting the bacterial solution into an incubator for culturing, setting the temperature of the incubator to be 28-32 ℃, setting the oscillation frequency to be 200-320 rpm, and setting the culture time to be 48-72 h;
step 1.3, measuring the light absorption value (OD) of the bacterial liquid obtained in the step 1.2 at the wavelength of 600nm600Value at OD600Taking out the blank when the blank is 1.2-2.0;
step 1.4, placing a calcium chloride and urea solution with a molar ratio of 50: 50 in a beaker, adding deionized water, stirring, and dissolving to form a cementing solution, wherein the mass concentrations of the calcium chloride and the urea in the cementing solution are respectively 1.0%:
step 2, setting the curing type of surface soil of red layer side slope
Recording the solidification of the small micro cracks of the surface soil of the red slope as solidification 1, recording the solidification of the surface soil of the red slope as solidification 2, and recording the comprehensive solidification of the small micro cracks and the surface of the surface soil of the red slope as solidification 3, wherein the solidification of the surface soil of the red slope comprises any one or the combination of two or more of solidification 1, solidification 2 and solidification 3;
the concrete step of curing 1 is step 3, and the concrete step of curing 2 is step 4; the concrete step of curing 3 is step 5;
step 3, curing 1
Step 3.1, preparing spraying liquid 1 for red layer side slope surface soil per square meter according to the volume ratio of 4400-6000 ml of cementing liquid to 4400-6000 ml of bacterial liquid;
step 3.2, uniformly spraying the spraying liquid 1 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 48-96 hours;
step 3.3, taking a proper amount of soil sample from the red layer slope site solidified in the step 3.2, recording the soil sample as a micro-crack solidified soil sample, sealing and storing the micro-crack solidified soil sample in a laboratory, and detecting the permeability coefficient delta of the micro-crack solidified soil sample1：
If delta1Of the order of > 10-7Returning to the step 3.2;
if delta1Of order of 10 or less-7When the current curing meets the requirements, the curing 1 is finished;
step 4, curing 2
Step 4.1, preparing spraying liquid 2 for the surface soil of the red layer side slope per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 6000 ml-8000 ml to 6000 ml-8000 ml;
step 4.2, uniformly spraying the spraying liquid 2 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 60-96 hours;
step 4.3, taking a proper amount of soil sample from the red layer slope site solidified in the step 4.2, recording the soil sample as a surface solidified soil sample, sealing and storing the soil sample in a laboratory, and detecting the permeability coefficient delta of the surface solidified soil sample2：
If delta2Of the order of > 10-7Returning to the step 4.2;
if delta2Of order of 10 or less-7When the current curing meets the requirements, the curing 2 is finished;
step 5, curing 3
Step 5.1, preparing spraying liquid 3 for the surface soil of the red layer side slope per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 8000 ml-10000 ml to 8000 ml-10000 ml;
step 5.2, uniformly spraying the spraying liquid 3 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 72-96 hours;
step 5.3, taking a proper amount of soil sample from the red layer slope site solidified in the step 5.2, recording the soil sample as a comprehensive solidified soil sample, sealing and storing the soil sample in a laboratory, and detecting the permeability coefficient delta of the comprehensive solidified soil sample3：
If delta3Of the order of > 10-7Returning to the step 5.2;
if delta3Of order of 10 or less-7And the curing of the time meets the requirements, and the curing 3 is finished.
Preferably, the American type culture Collection recommended formula ATCC 1376NH4The composition of the culture broth of the YE configuration was: 20g yeast extract, 10g (NH)4)2SO40.13mol/L Tris Buffer, deionized water to 1L, and 1mol/L HCl to adjust the pH of the culture solution to 9.0.
Preferably, the bacillus pasteurii is american type culture collection number ATCC 11859.
Compared with the prior art, the beneficial effects of the invention comprise:
1. short curing period, good curing effect, simple process, high speed and efficiency, easy culture and rapid propagation of microorganisms and simple spraying process.
2. The applicability is strong, the environmental tolerance is good, and the paint can be used for a plurality of extreme environmental conditions, such as acid environment, alkaline environment and the like;
3. the resources are rich, and the natural microbial resources are fully utilized;
4. green and clean, and environment-friendly.
Drawings
FIG. 1 is a flow chart of the present invention.
FIG. 2 is a graph showing the change of permeability coefficient with respect to the standing time in example 2 of the present invention.
Detailed Description
The process of the present invention is described in further detail below with reference to examples.
Example 1, fig. 1 is a flow chart of the present invention, from which it can be seen that a method for solidifying topsoil of a red layer slope by using a microbial preparation of the present invention comprises the following steps:
step 1, preparation of bacterial liquid and cementing liquid
Step 1.1, adopting American type culture Collection to recommend a formula ATCC 1376NH4Preparing a culture solution by YE, sterilizing the culture solution at 121-123 ℃ for 20-30 min, placing the culture solution on an ultra-clean workbench for ultraviolet sterilization and ventilation, and standing by when the temperature of the culture solution is reduced to room temperature.
In this example, the culture broth was sterilized at 121 ℃ for 30 min.
In this example, the American type culture Collection recommended formula ATCC 1376NH4The composition of the culture broth of the YE configuration was: 20g yeast extract, 10g (NH)4)2SO40.13mol/L Tris Buffer, deionized water to 1L, and 1mol/L HCl to adjust the pH of the culture solution to 9.0.
Step 1.2, after the temperature of the culture solution is reduced to room temperature, taking the culture solution into a container, adding the pasteurella bacillus into the culture solution by using a sterile pipette to obtain a bacterial solution, wherein the volume ratio of the pasteurella bacillus to the culture solution is 1: 100, putting the bacterial solution into an incubator for culturing, the temperature of the incubator is set to be 28-32 ℃, the oscillation frequency is set to be 200-320 rpm, and the culture time is 48-72 h.
In this example, the Bacillus pasteurianus is American type culture Collection number ATCC 11859. In this example, the temperature of the incubator was set to 31 ℃, the oscillation frequency was set to 230rpm, and the incubation time was set to 48 hours.
Step 1.3, measuring the light absorption value (OD) of the bacterial liquid obtained in the step 1.2 at the wavelength of 600nm600Value at OD600And taking out the blank when the blank is 1.2-2.0 for later use.
In this example, OD of bacterial suspension600When the ratio is 1.8, the extract is taken out for use.
Step 1.4, placing a calcium chloride and urea solution with a molar ratio of 50: 50 in a beaker, adding deionized water, stirring, and dissolving to form a cementing solution, wherein the mass concentrations of the calcium chloride and the urea in the cementing solution are respectively 1.0%.
Step 2, setting the curing type of surface soil of red layer side slope
And recording the solidification of the surface soil micro cracks of the red slope as solidification 1, recording the solidification of the surface soil of the red slope as solidification 2, and recording the comprehensive solidification of the surface soil micro cracks and the surface of the red slope as solidification 3, wherein the solidification of the surface soil of the red slope comprises any one or the combination of two or more of solidification 1, solidification 2 and solidification 3.
The concrete step of curing 1 is step 3, and the concrete step of curing 2 is step 4; the specific step of curing 3 is step 5.
Step 3, curing 1
And 3.1, preparing spraying liquid 1 for red layer slope surface soil per square meter according to the volume ratio of 4400-6000 ml of cementing liquid to 4400-6000 ml of bacterial liquid.
In the embodiment, the spraying liquid 1 is prepared from red layer slope surface soil per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 4500ml to 4500 ml.
And 3.2, uniformly spraying the spraying liquid 1 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 48-96 hours.
In this embodiment, utilize unmanned aerial vehicle will spray liquid 1 evenly to spray to red layer slope topsoil on, the 48h of stewing.
Step 3.3, taking a proper amount of soil sample from the red layer slope site solidified in the step 3.2, recording the soil sample as a micro-crack solidified soil sample, sealing and storing the micro-crack solidified soil sample in a laboratory, and detecting the permeability coefficient delta of the micro-crack solidified soil sample1：
If delta1Of the order of > 10-7Returning to the step 3.2;
if delta1Of order of 10 or less-7The curing meets the requirementsAnd curing 1 is finished.
Step 4, curing 2
And 4.1, preparing a spraying liquid 2 for the surface soil of the red layer side slope per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 6000 ml-8000 ml to 6000 ml-8000 ml.
In the embodiment, spraying liquid 2 is prepared by the volume ratio of the cementing liquid to the bacterial liquid of 6000ml to 6000ml for the surface soil of the red layer slope per square meter.
And 4.2, uniformly spraying the spraying liquid 2 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 60-96 hours.
In this embodiment, in waiting the red layer side slope place of solidification, utilize unmanned aerial vehicle will spray liquid 2 evenly and spray to red layer side slope topsoil on, the 60 hours of stewing.
Step 4.3, taking a proper amount of soil sample from the red layer slope site solidified in the step 4.2, recording the soil sample as a surface solidified soil sample, sealing and storing the soil sample in a laboratory, and detecting the permeability coefficient delta of the surface solidified soil sample2：
If delta2Of the order of > 10-7Returning to the step 4.2;
if delta2Of order of 10 or less-7And the curing 2 is finished when the curing meets the requirements.
Step 5, curing 3
And 5.1, preparing spraying liquid 3 for the surface soil of the red layer side slope per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 8000 ml-10000 ml to 8000 ml-10000 ml.
In this embodiment, the spraying liquid 3 is prepared from the surface soil of the red layer slope per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 8000ml to 8000 ml.
And 5.2, uniformly spraying the spraying liquid 3 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 72-96 hours.
In this embodiment, in waiting the red layer side slope place of solidification, utilize unmanned aerial vehicle will spray liquid 3 and evenly spray to red layer side slope topsoil on, the 72 ~ 96h that stews.
Step 5.3, taking the cured red layer slope field in the step 5.2Measuring and marking the soil sample as a comprehensive solidified soil sample, sealing and storing the soil sample in a laboratory, and detecting the permeability coefficient delta of the comprehensive solidified soil sample3：
If delta3Of the order of > 10-7Returning to the step 5.2;
if delta3Of order of 10 or less-7And the curing of the time meets the requirements, and the curing 3 is finished.
Example 2: take solidifying red layer slope soil at a certain place of Anhui province as an example.
Through the detection of sampling the surface soil of the red layer side slope of the field, the permeability coefficient delta of the surface soil of the red layer side slope0Is 1.5X 10-3I.e. orders of magnitude much greater than > 10-7The topsoil of the red layer slope needs to be comprehensively cured, namely, the topsoil surface needs to be repaired through the curing 3 in the embodiment 1. The method comprises the following specific steps:
step 1, preparation of bacterial liquid and cementing liquid
Same as in example 1.
Step 2, solidifying topsoil of red layer side slope
Spraying liquid 2 is prepared for the surface soil of the red layer side slope per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 8000ml to 8000 ml.
In the red layer side slope field to be solidified, spraying liquid 2 is uniformly sprayed onto the surface soil of the red layer side slope by using an unmanned aerial vehicle, and standing for 96 hours.
And after the standing time is started, taking the surface solidified soil sample in the solidified field for multiple times, sealing and storing the surface solidified soil sample in a laboratory, and detecting the permeability coefficient delta of the surface solidified soil sample. Fig. 2 shows the delta as a function of the resting time. As can be seen from the curve, the initial value of the permeability coefficient is 1.50X 10-3cm/s and a permeability coefficient delta of 8.71 multiplied by 10 after 24h of curing-4cm/s and a permeability coefficient delta of 6.52X 10 at 60h after curing-7cm/s, and finally the permeability coefficient delta reaches 4.17 multiplied by 10 at 72h-7cm/s。
The great reduction of the permeability coefficient delta shows that the method has a very obvious effect on solidifying the surface soil of the red layer slope field, the permeability coefficient is continuously reduced along with the increase of the standing time, a hardened layer is formed on the surface of the red layer slope field, and the influence of rainwater on the surface soil of the red layer slope is reduced, so that the geological disasters such as collapse, landslide and the like of the surface of the red layer slope are reduced, the safety of production and construction is guaranteed, and the life and property safety of people is guaranteed.
Claims (3)
1. A method for solidifying topsoil of a red layer slope by using a microbial preparation is characterized by comprising the following steps:
step 1, preparation of bacterial liquid and cementing liquid
Step 1.1, adopting American type culture Collection to recommend a formula ATCC 1376NH4Preparing a culture solution by YE, sterilizing the culture solution at 121-123 ℃ for 20-30 min, placing the culture solution on an ultra-clean workbench for ultraviolet sterilization and ventilation, and cooling the culture solution to room temperature for later use;
step 1.2, after the temperature of the culture solution is reduced to room temperature, taking the culture solution into a container, adding the pasteurella bacillus into the culture solution by using a sterile pipette to obtain a bacterial solution, wherein the volume ratio of the pasteurella bacillus to the culture solution is 1: 100, putting the bacterial solution into an incubator for culturing, setting the temperature of the incubator to be 28-32 ℃, setting the oscillation frequency to be 200-320 rpm, and setting the culture time to be 48-72 h;
step 1.3, measuring the light absorption value (OD) of the bacterial liquid obtained in the step 1.2 at the wavelength of 600nm600Value at OD600Taking out the blank when the blank is 1.2-2.0;
step 1.4, placing a calcium chloride and urea solution with a molar ratio of 50: 50 in a beaker, adding deionized water, stirring, and dissolving to form a cementing solution, wherein the mass concentrations of the calcium chloride and the urea in the cementing solution are respectively 1.0%;
step 2, on-site detection and setting of red layer slope surface soil solidification type
Recording the solidification of the small micro cracks of the surface soil of the red slope as solidification 1, recording the solidification of the surface soil of the red slope as solidification 2, and recording the comprehensive solidification of the small micro cracks and the surface of the surface soil of the red slope as solidification 3, wherein the solidification of the surface soil of the red slope comprises any one or the combination of two or more of solidification 1, solidification 2 and solidification 3;
the concrete step of curing 1 is step 3, and the concrete step of curing 2 is step 4; the concrete step of curing 3 is step 5;
step 3, curing 1
Step 3.1, preparing spraying liquid 1 for red layer side slope surface soil per square meter according to the volume ratio of 4400-6000 ml of cementing liquid to 4400-6000 ml of bacterial liquid;
step 3.2, uniformly spraying the spraying liquid 1 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 48-96 hours;
step 3.3, taking a proper amount of soil sample from the red layer slope site solidified in the step 3.2, recording the soil sample as a micro-crack solidified soil sample, sealing and storing the micro-crack solidified soil sample in a laboratory, and detecting the permeability coefficient delta of the micro-crack solidified soil sample1：
If delta1Of the order of > 10-7Returning to the step 3.2;
if delta1Of order of 10 or less-7When the current curing meets the requirements, the curing 1 is finished;
step 4, curing 2
Step 4.1, preparing spraying liquid 2 for the surface soil of the red layer side slope per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 6000 ml-8000 ml to 6000 ml-8000 ml;
step 4.2, uniformly spraying the spraying liquid 2 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 60-96 hours;
step 4.3, taking a proper amount of soil sample from the red layer slope site solidified in the step 4.2, recording the soil sample as a surface solidified soil sample, sealing and storing the soil sample in a laboratory, and detecting the permeability coefficient delta of the surface solidified soil sample2：
If delta2Of the order of > 10-7Returning to the step 4.2;
if delta2Of order of 10 or less-7When the current curing meets the requirements, the curing 2 is finished;
step 5, curing 3
Step 5.1, preparing spraying liquid 3 for the surface soil of the red layer side slope per square meter according to the volume ratio of the cementing liquid to the bacterial liquid of 8000 ml-10000 ml to 8000 ml-10000 ml;
step 5.2, uniformly spraying the spraying liquid 3 on the surface soil of the red layer slope by using an unmanned aerial vehicle in the red layer slope field to be solidified, and standing for 72-96 hours;
step 5.3, taking a proper amount of soil sample from the red layer slope site solidified in the step 5.2, recording the soil sample as a comprehensive solidified soil sample, sealing and storing the soil sample in a laboratory, and detecting the permeability coefficient delta of the comprehensive solidified soil sample3：
If delta3Of the order of > 10-7Returning to the step 5.2;
if delta3Of order of 10 or less-7And the curing of the time meets the requirements, and the curing 3 is finished.
2. The method for solidifying topsoil of redbed slope with microbial preparation as claimed in claim 1, wherein said American type culture Collection recommended formula ATCC 1376NH4The composition of the culture broth of the YE configuration was: 20g yeast extract, 10g (NH)4)2SO40.13mol/L Tris Buffer, deionized water to 1L, and 1mol/L HCl to adjust the pH of the culture solution to 9.0.
3. The method for solidifying topsoil of redbed slope with a microbial preparation according to claim 1, wherein the bacillus pasteurii is american type culture collection number ATCC 11859.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110416256.0A CN113123353A (en) | 2021-04-16 | 2021-04-16 | Method for solidifying redbed slope surface soil by microbial preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110416256.0A CN113123353A (en) | 2021-04-16 | 2021-04-16 | Method for solidifying redbed slope surface soil by microbial preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113123353A true CN113123353A (en) | 2021-07-16 |
Family
ID=76777575
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110416256.0A Pending CN113123353A (en) | 2021-04-16 | 2021-04-16 | Method for solidifying redbed slope surface soil by microbial preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113123353A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114482087A (en) * | 2021-12-23 | 2022-05-13 | 合肥工业大学 | Method for solidifying side slope by microorganism mineralization filling-magnesia carbonization guniting |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105623671A (en) * | 2015-12-29 | 2016-06-01 | 吕贵松 | Water-resisting soil stabilizer |
| CN105622016A (en) * | 2015-12-29 | 2016-06-01 | 吕贵松 | Composite soil solidifying agent |
| US20170183568A1 (en) * | 2015-12-23 | 2017-06-29 | King Fahd University Of Petroleum And Minerals | Method for reducing swell potential of expansive clayey soil with nano-level constitutive modeling |
| JP2018150477A (en) * | 2017-03-14 | 2018-09-27 | 日本基礎技術株式会社 | Grout material and grout injection method |
| CN109433443A (en) * | 2018-11-30 | 2019-03-08 | 河海大学 | A kind of sandy soil solidification equipment and control method based on unmanned plane |
| CN109778834A (en) * | 2019-01-09 | 2019-05-21 | 南京大学 | A method of utilizing microbial control soil body dry shrinkage and cracking |
| CN110330251A (en) * | 2019-07-16 | 2019-10-15 | 中山大学 | Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising |
| CN110451769A (en) * | 2019-08-21 | 2019-11-15 | 北京本农科技发展有限公司 | A kind of method of curing agent and preparation method thereof, slurry curing |
| CN111254946A (en) * | 2020-01-16 | 2020-06-09 | 西北农林科技大学 | Method for reinforcing and protecting loess slope surface based on microbial mineralization |
| CN111501733A (en) * | 2020-04-21 | 2020-08-07 | 南京大学 | Method for solidifying soil body by utilizing in-situ microorganisms cultured in excitation mode |
| CN111535342A (en) * | 2020-05-15 | 2020-08-14 | 北华航天工业学院 | Method and device for reinforcing sandy cutting high slope by combining microorganisms and anchor cables |
| CN111672900A (en) * | 2020-06-01 | 2020-09-18 | 合肥工业大学 | Method for restoring heavy metal pollution of soil by microorganism-induced calcium carbonate precipitation |
-
2021
- 2021-04-16 CN CN202110416256.0A patent/CN113123353A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170183568A1 (en) * | 2015-12-23 | 2017-06-29 | King Fahd University Of Petroleum And Minerals | Method for reducing swell potential of expansive clayey soil with nano-level constitutive modeling |
| CN105623671A (en) * | 2015-12-29 | 2016-06-01 | 吕贵松 | Water-resisting soil stabilizer |
| CN105622016A (en) * | 2015-12-29 | 2016-06-01 | 吕贵松 | Composite soil solidifying agent |
| JP2018150477A (en) * | 2017-03-14 | 2018-09-27 | 日本基礎技術株式会社 | Grout material and grout injection method |
| CN109433443A (en) * | 2018-11-30 | 2019-03-08 | 河海大学 | A kind of sandy soil solidification equipment and control method based on unmanned plane |
| CN109778834A (en) * | 2019-01-09 | 2019-05-21 | 南京大学 | A method of utilizing microbial control soil body dry shrinkage and cracking |
| CN110330251A (en) * | 2019-07-16 | 2019-10-15 | 中山大学 | Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising |
| CN110451769A (en) * | 2019-08-21 | 2019-11-15 | 北京本农科技发展有限公司 | A kind of method of curing agent and preparation method thereof, slurry curing |
| CN111254946A (en) * | 2020-01-16 | 2020-06-09 | 西北农林科技大学 | Method for reinforcing and protecting loess slope surface based on microbial mineralization |
| CN111501733A (en) * | 2020-04-21 | 2020-08-07 | 南京大学 | Method for solidifying soil body by utilizing in-situ microorganisms cultured in excitation mode |
| CN111535342A (en) * | 2020-05-15 | 2020-08-14 | 北华航天工业学院 | Method and device for reinforcing sandy cutting high slope by combining microorganisms and anchor cables |
| CN111672900A (en) * | 2020-06-01 | 2020-09-18 | 合肥工业大学 | Method for restoring heavy metal pollution of soil by microorganism-induced calcium carbonate precipitation |
Non-Patent Citations (2)
| Title |
|---|
| 焦隆新等: "微生物诱导碳酸钙沉积技术加固红层黏土夹层的试验", 《工业建筑》 * |
| 现行公路施工及验收技术规范大全编委会: "《现行公路施工及验收技术规范大全 （上卷）》", 30 September 2001 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114482087A (en) * | 2021-12-23 | 2022-05-13 | 合肥工业大学 | Method for solidifying side slope by microorganism mineralization filling-magnesia carbonization guniting |
| CN114482087B (en) * | 2021-12-23 | 2024-05-07 | 合肥工业大学 | Method for curing side slope by microorganism mineralization filling-magnesia carbonization slurry spraying |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lai et al. | Experimental study to improve the mechanical properties of iron tailings sand by using MICP at low pH | |
| CN108975787B (en) | Microbial cementing filling material for coal mine and preparation method thereof | |
| CN101644047A (en) | Method of adhering loose sands employing microbial mineralization | |
| CN108220196B (en) | Method for treating petroleum-polluted clay foundation by using microbial composite flora | |
| CN103266070A (en) | Bacillus lentus and method for generating by inducing bacillus lentus for reinforcing foundation | |
| CN103820123A (en) | Microbial sand-fixing dust depressor and preparation method thereof | |
| CN101302484A (en) | Method for recovering cement-based material crack by means of microorganism, culture fluid and repair nutrient fluid | |
| CN110565625B (en) | Method for solidifying island calcareous sand by using primary microorganisms | |
| CN105040675A (en) | Sandy soil microbe solidification method and apparatus | |
| CN109610658A (en) | A method of utilizing microorganism induction tosca repairing concrete crack | |
| Liu et al. | Geotechnical engineering properties of soils solidified by microbially induced CaCO3 precipitation (MICP) | |
| CN105524620A (en) | A novel soil stabilizer | |
| CN109162684A (en) | A kind of method that polymer microballoon/endogenous microbes/biology surfactant ternary composite driving improves low-permeability oil deposit oil recovery factor | |
| CN113123353A (en) | Method for solidifying redbed slope surface soil by microbial preparation | |
| CN110330251A (en) | Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising | |
| CN107309270B (en) | Application of BB (BB bacteria) in reduction of pH value of red mud | |
| CN109576193B (en) | Microbial remediation liquid and application method thereof in protection of sandy foundation pit slope | |
| CN105295844A (en) | Clean soil-stabilizing and dust-suppression agent and preparation method thereof | |
| CN104402287B (en) | A kind of method for refinforced cement sill alkali burnout resistance energy | |
| CN110395884A (en) | A kind of bionical self-repair concrete and preparation method thereof | |
| CN105926964B (en) | A kind of method using thermophilic cold carbonate mineralized bacterium rehabilitating concrete surface | |
| CN103789242A (en) | Arthobacter niigatensis and method for improving liquefaction characteristics of silty soil by utilizing arthobacter niigatensis | |
| CN112779182A (en) | Portable microorganism-induced calcium carbonate precipitation kit and application thereof in repairing early cracks of pavement base | |
| CN115749931A (en) | Tailing sand biological cemented filling method and system based on bacterial urease | |
| Barberán et al. | A new area of application and research in bio-processes: Biotechnologies in civil construction |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210716 |
|
| RJ01 | Rejection of invention patent application after publication |