CN109610658A - A method of utilizing microorganism induction tosca repairing concrete crack - Google Patents
A method of utilizing microorganism induction tosca repairing concrete crack Download PDFInfo
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- CN109610658A CN109610658A CN201811557680.1A CN201811557680A CN109610658A CN 109610658 A CN109610658 A CN 109610658A CN 201811557680 A CN201811557680 A CN 201811557680A CN 109610658 A CN109610658 A CN 109610658A
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- E—FIXED CONSTRUCTIONS
- E04—BUILDING
- E04B—GENERAL BUILDING CONSTRUCTIONS; WALLS, e.g. PARTITIONS; ROOFS; FLOORS; CEILINGS; INSULATION OR OTHER PROTECTION OF BUILDINGS
- E04B1/00—Constructions in general; Structures which are not restricted either to walls, e.g. partitions, or floors or ceilings or roofs
- E04B1/62—Insulation or other protection; Elements or use of specified material therefor
- E04B1/66—Sealings
- E04B1/68—Sealings of joints, e.g. expansion joints
- E04B1/6801—Fillings therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of methods using microorganism induction tosca repairing concrete crack, the following steps are included: preparing the synthetic media of liquid, the sodium hydroxide solution for configuring 0.1mol/L corrects medium pH=9.0 ~ 10.0, Bacillus pasteurii is inoculated into culture medium, it is put into constant temperature oscillation case, 30 DEG C of temperature setting, 200r/min is arranged in revolving speed, it is cultivated, whole process keeps germ-free condition；Cultured bacterium solution and urea are mixed, a period of time is stood, then by mixed liquor and calcium salt soln simultaneously trickle irrigation into distress in concrete.Compared with the restorative procedure of conventional concrete crack, the present invention, which handles distress in concrete, has timeliness fast, the high advantage of intensity.
Description
Technical field
The invention belongs to building field, in particular to a kind of methods using microorganism remediation construction material crack.
Background technique
In recent years, continuous with the continuous development of building waterproofing technique and China's buildings waterproof standard criterion, regulation etc.
Perfect, the leakage ratio of China's building construction constantly declines, and national, large area leakage problems are tentatively contained,
But the leakage problems of hypogee still allow of no optimist.The report of the publication of Chinese architecture waterproof association in 2014 shows that the whole nation has
28 urban architecture underground leakage rates are up to 57.51%.The reason of distress in concrete formation, is varied, including design, material
And match ratio, construction and scene conserve and use.The formation and development in crack gently then influences the normal use of building,
It is heavy then cause serious life and property loss.The form and the origin cause of formation of judgement and analysis distress in concrete, form corresponding Zhuo Youcheng
The crack reclamation activities of effect is control and the effective way for reducing distress in concrete.In recent years, for distress in concrete reparation
New technology, new material also continuously emerge, find more environmentally protective, the better distress in concrete restorative procedure of durability at
For the research emphasis of technical staff.The Crack cause complexity of distress in concrete is various, can substantially be divided into two classes: one is by
Crack caused by load action, referred to as load Crack.Load Crack mainly has two kinds of direct crack and secondary stress crack.Directly
The crack that the direct stress that crack refers to that external load generates generates is connect, secondary stress crack refers to that the secondary stress generated by load is drawn
The crack risen.Crack caused by load reason accounts for about 20% or so.Another kind be because shrinking, creeping, the coagulations such as differential settlement
Crack caused by soil deforms, referred to as deformation crack.Since concrete has the characteristics that expand with heat and contract with cold, when concrete or outside
When boundary's environment temperature changes, concrete can be expanded or be shunk, will be when the deformation of concrete suffers restraints
Stress is generated in concrete, if stress is more than the tensile strength of concrete, will generate thermal cracking, such crack is in reality
It is relatively conventional in the engineering of border.In addition, vertically not shaking up sedimentation or transversely deforming due to ground, cause to generate in structure additional
Stress, the tensile strength more than concrete will generate crack in concrete.Above several cracks belong to deformation crack,
Such crack accounts for about 80%.
During repairing concrete crack, how to enhance urease activity, the early stage yield of precipitation of calcium carbonate is improved, in phase
It closes in research also without specific method, the method reported is not directed to the survey of the characterization of micro organism quantity, urease activity
Fixed and raising problem.In addition, in the research using microbial technique repairing concrete crack also rare report.
Summary of the invention
In order to solve problem above, it is repaired the object of the present invention is to provide a kind of using using microorganism induction tosca
The method of multiple distress in concrete.
The present invention realizes object above, the technical solution of the present invention is as follows:
A method of utilizing microorganism induction tosca repairing concrete crack, it is characterised in that: including following
Step:
(1) fluid nutrient medium is prepared, sodium hydroxide solution correction medium pH=9.0~10.0 of 0.1mol/L are configured,
Bacillus pasteurii is inoculated into the culture medium after sterilizing, is trained in 30~35 DEG C, the constant temperature oscillation case of revolving speed 200r/min
Support 1d~2d；
(2) cultured bacterium solution and urea are mixed, stands 1~1.5h and obtains mixed liquor；
(3) distress in concrete is rinsed with clean water, fills normal sands or quartz sand；
(4) by mixed liquor and calcium salt soln, trickle irrigation is into distress in concrete simultaneously, until distress in concrete reparation is completed.
Further, the microbiological culture media includes: Yeast extract 20g/L, NH4Cl 10g/L、MnSO4·
H2O 10mg/L、NiCl2·6H2O 1.2mg/L and pure water.
Further, in step (1), when the pH=9 of fluid nutrient medium, the PH=9 of fluid nutrient medium, enzymatic activity highest.
Further, in step (1), spectrophotometer OD is used after shaken cultivation 1d~2d600Method characterize biology
Amount, for OD600 value to 0.160~0.180, micro organism quantity excessively causes conglomeration to will affect absorbance measurement after bacterium solution dilution,
Concentration is lower, leads to absorbance change no significant difference.Therefore, it chooses suitable concentration and is conducive to microorganism characterization, choose and close
Suitable microorganism growing step.
Further, in step (2), cultured bacteria solution active is 2.8mS/cm/min by comparing different algebra enzymes
Active variation continues culture discovery enzymatic activity and is maintained at a metastable numerical value after enzymatic activity reaches certain peak value,
Numerical value at this time maintains 2.8 ms/cm/min, and urase hydrolyzes urea and generates carbanion and ammonium ion, increases solution
Conductivity.The amount of hydrolysis of urea and the variable quantity of electrical conductivity of solution are directly proportional, therefore can use the variation scale of electrical conductivity of solution
Levy the urease activity of microorganism.
Further, in step (3), according to fracture width depth, the normal sands for selecting corresponding stage to match or quartz sand as
Filled media.
Further, in step (4), the proportion of bacterium solution and calcium salt is 1:1.5；Urea content is 15g/100ml, and in advance
It is added in bacterium solution within 1 hour, oscillation, which shakes up, dissolves it sufficiently.
Further, in step (4), calcium salt soln is one of calcium chloride, calcium nitrate or calcium acetate or a variety of.
Further, in step (4), calcium concentration 1.0mol/L.When calcium concentration is higher than 1.0mol/L, calcium carbonate
Deposition is without rising appreciably.
The invention has the advantages that restorative procedure of the invention has wide excellent of repair coverage relative to traditional restorative procedure
Point can all repair big small crack, while intensity is high after reparation, and newly-generated calcium carbonate rain protocorm combination is more tight
It is close, it is also more preferable for the crack repairing effect of deep layer.
Specific embodiment
The contents of the present invention are further described in detail by the following examples.
Embodiment 1
Fluid nutrient medium is prepared, microbiological culture media includes: Yeast extract 20g/L, NH4Cl 10g/L、
MnSO4·H2O 10mg/L、NiCl2·6H2O 1.2mg/L and pure water configure the sodium hydroxide solution correction training of 0.1mol/L
Base pH=9.0 is supported, Bacillus pasteurii is inoculated into the culture medium after sterilizing, at 30 DEG C, the constant temperature of revolving speed 200r/min shakes
It swings and cultivates 1d in case；By cultured OD600Value is that 0.160 bacterium solution and urea mix, and standing 1h and obtaining bacteria solution active is 2.8m
The mixed liquor of S/cm/min；Distress in concrete is rinsed with clean water, according to fracture width depth, corresponding stage is selected to match
Normal sands or quartz sand are as filled media；By mixed liquor and calcium salt soln simultaneously trickle irrigation into distress in concrete, wherein bacterium solution
Proportion with calcium salt is 1:1.5；Urea content is 15g/100ml, and urea is shifted to an earlier date 1 hour and is added in bacterium solution, calcium salt soln
It is one of calcium chloride, calcium nitrate or calcium acetate or a variety of, calcium concentration 1.0mol/L is added to bacterium in 1 hour in advance
In liquid, oscillation, which shakes up, dissolves it sufficiently.Until distress in concrete reparation is completed.
Embodiment 2
The present embodiment is not all Bacillus pasteurii to be inoculated into the culture medium after sterilizing, 30 with embodiment 1
DEG C, it is cultivated 1.5 days in the constant temperature oscillation case of revolving speed 200r/min.
Embodiment 3
The present embodiment is not all Bacillus pasteurii to be inoculated into the culture medium after sterilizing, 30 with embodiment 1
DEG C, it is cultivated 2 days in the constant temperature oscillation case of revolving speed 200r/min.
Embodiment 4
The present embodiment is not all that OD600 value is 0.17 after bacterium solution dilution with embodiment 1.
Embodiment 5
The present embodiment is not all that OD600 value is 0.18 after bacterium solution dilution with embodiment 1.
Comparative example 1
This comparative example and embodiment 1 are not all medium pH=5.0.
Comparative example 2
This comparative example and embodiment 1 are not all medium pH=7.0.
Comparative example 3
This comparative example and embodiment 1 are not all medium pH=11.0.
Comparative example 4
This comparative example and embodiment 1 are not all medium pH=13.0.
Comparative example 5
This comparative example is not all that urea is shifted to an earlier date 20min and is added in bacterium solution with embodiment 1.
Comparative example 6
This comparative example is not all that urea is shifted to an earlier date 40min and is added in bacterium solution with embodiment 1.
Comparative example 7
The reparation in crack is carried out using conventional method.
Table 1~3 is the experimental result test of embodiment and comparative example.
Influence of the pH value to urease activity
Table 1
The influence for the bacterium solution time rate being generated to calcium carbonate is added in urea
| The time is added in advance | Comparative example 5 | Comparative example 6 | Embodiment 1 |
| CaCO3Yield (g) | 8.20 | 9.83 | 12.5 |
Table 2
With the contrast test of traditional restorative procedure
Table 3
By comparison, it can be seen that, restorative procedure provided by the invention has better repairing effect, the process ring of reparation
Energy conservation is protected, while also there is higher intensity and impervious carbonization resistance after repairing, there is better effect.
Claims (9)
1. a kind of method using microorganism induction tosca repairing concrete crack, it is characterised in that: including following step
It is rapid:
Fluid nutrient medium is prepared, sodium hydroxide solution correction medium pH=9.0 ~ 10.0 of 0.1mol/L are configured, by Pasteur's bud
Spore bacillus is inoculated into the culture medium after sterilizing, and 1d ~ 2d is cultivated in 30 ~ 35 DEG C, the constant temperature oscillation case of revolving speed 200r/min；
Cultured bacterium solution and urea are mixed, 1 ~ 1.5h is stood and obtains mixed liquor；
Distress in concrete is rinsed with clean water, fills normal sands or quartz sand；
By mixed liquor and calcium salt soln, trickle irrigation is into distress in concrete simultaneously, until distress in concrete reparation is completed.
2. the method for utilizing microorganism induction tosca repairing concrete crack as described in claim 1, feature exist
In: in step (1), the microbiological culture media includes: Yeast extract 20g/L, NH4Cl 10g/L、MnSO4·H2O
10mg/L、NiCl2·6H2O 1.2mg/L and pure water.
3. the method for utilizing microorganism induction tosca repairing concrete crack as described in claim 1, feature exist
In: in step (1), pH=9 of fluid nutrient medium.
4. the method for utilizing microorganism induction tosca repairing concrete crack as described in claim 1, feature exist
In: in step (1), spectrophotometer OD is used after shaken cultivation 1d ~ 2d600Method characterize biomass, bacterium solution dilution after
OD600It is worth to 0.160 ~ 0.180.
5. the method for utilizing microorganism induction tosca repairing concrete crack as described in claim 1, feature exist
In: in step (2), cultured bacteria solution active is 2.8 m S/cm/min.
6. the method for utilizing microorganism induction tosca repairing concrete crack as described in claim 1, feature exist
In: in step (3), according to fracture width depth, the normal sands for selecting corresponding stage to match or quartz sand are as filled media.
7. the method for utilizing microorganism induction tosca repairing concrete crack as described in claim 1, feature exist
In: in step (4), the proportion of bacterium solution and calcium salt is 1:1.5；Urea content is 15g/100ml, and 1 hour in advance is added to bacterium
In liquid, oscillation, which shakes up, dissolves it sufficiently.
8. the method for utilizing microorganism induction tosca repairing concrete crack as described in claim 1, feature exist
In: in step (4), calcium salt soln is one of calcium chloride, calcium nitrate or calcium acetate or a variety of.
9. the method for utilizing microorganism induction tosca repairing concrete crack as described in claim 1, feature exist
In: in step (4), calcium concentration is 1.0 mol/L.
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Cited By (9)
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| CN111877269A (en) * | 2020-06-24 | 2020-11-03 | 重庆大学 | Method for repairing poor rock mass in hydro-fluctuation belt of reservoir area by microorganisms |
| CN111922074A (en) * | 2019-06-03 | 2020-11-13 | 中国地质大学（北京） | Application of graphene oxide in microbial induction of calcium carbonate precipitation |
| CN112125592A (en) * | 2020-09-29 | 2020-12-25 | 广西壮族自治区水利科学研究院 | Microbial repairing agent for repairing rock cracks and preparation method thereof |
| CN112573941A (en) * | 2020-12-31 | 2021-03-30 | 武汉工程大学 | Method for repairing early cracks of cement stabilized macadam pavement base |
| CN113235567A (en) * | 2021-06-10 | 2021-08-10 | 西安建筑科技大学 | Method for solidifying soil relic cracks through microbial grouting |
| CN113307656A (en) * | 2021-05-29 | 2021-08-27 | 兰州理工大学 | Method for repairing concrete cracks by using microorganisms |
| CN113323441A (en) * | 2021-06-10 | 2021-08-31 | 重庆三峡学院 | Method for improving self-repairing width of concrete crack |
| CN113699072A (en) * | 2021-08-31 | 2021-11-26 | 北海市祥泰建设工程质量检测有限公司 | Automatic high-density culture method for bacillus pasteurii |
| CN115043669A (en) * | 2022-06-08 | 2022-09-13 | 湖南大学 | MICP (micro-induced plasticity) targeted repair method based on magnetic response |
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Cited By (9)
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|---|---|---|---|---|
| CN111922074A (en) * | 2019-06-03 | 2020-11-13 | 中国地质大学（北京） | Application of graphene oxide in microbial induction of calcium carbonate precipitation |
| CN111877269A (en) * | 2020-06-24 | 2020-11-03 | 重庆大学 | Method for repairing poor rock mass in hydro-fluctuation belt of reservoir area by microorganisms |
| CN112125592A (en) * | 2020-09-29 | 2020-12-25 | 广西壮族自治区水利科学研究院 | Microbial repairing agent for repairing rock cracks and preparation method thereof |
| CN112573941A (en) * | 2020-12-31 | 2021-03-30 | 武汉工程大学 | Method for repairing early cracks of cement stabilized macadam pavement base |
| CN113307656A (en) * | 2021-05-29 | 2021-08-27 | 兰州理工大学 | Method for repairing concrete cracks by using microorganisms |
| CN113235567A (en) * | 2021-06-10 | 2021-08-10 | 西安建筑科技大学 | Method for solidifying soil relic cracks through microbial grouting |
| CN113323441A (en) * | 2021-06-10 | 2021-08-31 | 重庆三峡学院 | Method for improving self-repairing width of concrete crack |
| CN113699072A (en) * | 2021-08-31 | 2021-11-26 | 北海市祥泰建设工程质量检测有限公司 | Automatic high-density culture method for bacillus pasteurii |
| CN115043669A (en) * | 2022-06-08 | 2022-09-13 | 湖南大学 | MICP (micro-induced plasticity) targeted repair method based on magnetic response |
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Inventor after: Meng Haining Inventor after: Zhang Shuai Inventor after: Ao Lin Inventor after: Lu Xiaojun Inventor after: Zhu Xiang Inventor after: Zuo Liping Inventor after: Xu Yanming Inventor before: Zhang Shuai Inventor before: Ao Lin Inventor before: Lu Xiaojun Inventor before: Zhu Xiang Inventor before: Zuo Liping Inventor before: Xu Yanming |
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Application publication date: 20190412 |