CN113235567A - Method for solidifying soil relic cracks through microbial grouting - Google Patents
Method for solidifying soil relic cracks through microbial grouting Download PDFInfo
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- CN113235567A CN113235567A CN202110649478.7A CN202110649478A CN113235567A CN 113235567 A CN113235567 A CN 113235567A CN 202110649478 A CN202110649478 A CN 202110649478A CN 113235567 A CN113235567 A CN 113235567A
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- 238000000034 method Methods 0.000 title claims abstract description 33
- 230000000813 microbial effect Effects 0.000 title claims abstract description 13
- 239000002689 soil Substances 0.000 title claims description 22
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 238000007789 sealing Methods 0.000 claims abstract description 14
- 239000011248 coating agent Substances 0.000 claims abstract description 11
- 238000000576 coating method Methods 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 11
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 34
- 241000894006 Bacteria Species 0.000 claims description 26
- 239000011440 grout Substances 0.000 claims description 24
- 239000001110 calcium chloride Substances 0.000 claims description 16
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 16
- 244000005700 microbiome Species 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 11
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 9
- 239000004202 carbamide Substances 0.000 claims description 9
- 230000005526 G1 to G0 transition Effects 0.000 claims description 8
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 230000001089 mineralizing effect Effects 0.000 claims description 8
- 108010046334 Urease Proteins 0.000 claims description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 10
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 230000033558 biomineral tissue development Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- 101000965313 Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) Aconitate hydratase A Proteins 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 239000004570 mortar (masonry) Substances 0.000 abstract 1
- 238000007711 solidification Methods 0.000 description 5
- 230000008023 solidification Effects 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 239000012779 reinforcing material Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
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- Engineering & Computer Science (AREA)
- Structural Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Agronomy & Crop Science (AREA)
- Mining & Mineral Resources (AREA)
- Paleontology (AREA)
- Civil Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Consolidation Of Soil By Introduction Of Solidifying Substances Into Soil (AREA)
Abstract
A method for solidifying the cracks of the earthen site by microbial grouting adopts the modes of sealing and coating sealing mortar around the cracks, transporting liquid by a peristaltic pump and distributing grouting, reduces the influence of the side wall effect in the grouting process and the problem of slow seepage of solution in a crack earthen site sample, has the advantages of easily obtained materials, simple operation, stronger compatibility of calcium carbonate generated by mineralization reaction and the original components of the earthen site, obvious effect of solidifying the cracks of the earthen site by the microbes step by step and provides a new idea for solidifying the cracks of the earthen site by the MICP technology.
Description
Technical Field
The invention belongs to the technical field of environmental geotechnics, and particularly relates to a method for solidifying a soil ruin crack by microbial grouting.
Background
The earthen site is a cultural heritage with high historical, artistic and scientific values and is mainly formed by ramming with soil. China's earthen sites are widely distributed and are distributed all over the country. Wherein most of the precious earthen sites are preserved in the northwest of China. However, the soil body is still the main material of the earthen site, the soil body is not strong in structure, and is easy to disintegrate in rain, the structure is loose and damaged, and the earthen site is damaged, wherein the cracks are main diseases affecting the stability of the loess earthen site. The existence of cracks on the site body has certain influence on the structure stability of the site body, and soil bodies on two sides can be softened when the site body is eroded by rainfall, so that the soil bodies on two sides of the cracks are dislocated and collapsed. The development and extension of the cracks can cause further damage to loess ruins, and great threat is generated to cultural relics.
In order to prevent further damage to the loess ruins, certain reinforcement measures need to be taken. Traditional chemical grouting such as resin materials belongs to organic reinforcing materials and has the characteristics of good soil body reinforcing strength and the like, but the characteristic of pore blocking enables the air permeability of a reinforced sample to be poor, and prevents water in a soil body from migrating and evaporating to the outside, so that secondary weathering damage of the soil body is easily caused.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a method for solidifying the cracks of the soil relics by microorganism grouting, which is a green and environment-friendly mineralization process for generating calcium carbonate by microorganism induction, can reduce the influence of the side wall effect in the grouting process and the problem that the seepage of solution in a crack soil relic sample is slow, and has the advantages of easy acquisition of test materials, easy operation and obvious effect of solidifying the cracks of the soil relics by microorganisms step by step; provides a new idea for curing the fracture of the earthen site by the MICP technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for solidifying a soil ruins crack by microorganism grouting specifically comprises the following steps:
1) adding 0.3g-0.45g of urea analytically pure into 10ml-15ml of bacterial liquid cultured for 20-30h in stationary phase for catalyzing for 15min-20min for later use;
2) sealing and coating the cracks of the earthen site sample within the crack opening degree of 1.2mm, reserving a grouting port at the top of the earthen site sample and above the cracks, and reserving a grout outlet at the bottom of the earthen site sample and below the cracks;
3) stably injecting the bacteria liquid catalyzed in the step 1) through a grouting port at the flow rate of 3-5 ml/min, enabling the bacteria liquid to enter cracks of the earthen site sample until the injected bacteria liquid flows out of a grout outlet, and stopping injection;
4) at a flow rate of 3ml/min-5ml/min, CaCl with a concentration of 0.5-1.5mol/L is stably injected through a grouting port2Solution of CaCl2The solution enters into the cracks of the earthen site sample until CaCl2The solution flows out from the grout outlet, stops injecting, and stands for 30min-1h to ensure that the bacteria liquid and CaCl in the crack2Fully mineralizing the solution;
5) repeating the steps 3) -4) for 8-10 times.
And 2) coating sealing daub on two sides of the crack for sealing.
3) the flow rate in the steps 4) is adjusted and controlled by a peristaltic pump; and the erosion of the earthen site sample by overhigh flow velocity is prevented.
The urease activity of the bacterial liquid obtained in the step 3) is 1.0-1.5.
Compared with the prior art, the invention has the following beneficial effects:
at present, most of the prior art is chemical grouting method for curing soil relic cracks, and no method for curing soil relic cracks by microorganism grouting is provided; the earthen site is mostly formed by tamping modified loess, lime components in the modified loess react with carbon dioxide in the atmosphere to generate calcium carbonate, and the calcium carbonate generated by the microorganism mineralization reaction does not conflict with calcium carbonate in the original earthen site in composition, so that the compatibility is good, and the earthen site can be better prevented from causing protective damage; according to the invention, the microbial grouting is adopted to solidify the soil relic crack, firstly, the improvement of the reaction liquid is carried out, urea is added into the bacterial liquid in advance for catalysis, so that the reaction rate is improved, the defect of the reaction rate of the two-phase grouting is made up, then, the grouting solidification is carried out by the two-phase grouting method, so that the microbial mineralization reaction of the two-phase grouting method is more thorough; meanwhile, the problems of influence of side wall effect and slow seepage of solution in the fractured earthen site sample in the grouting process are solved, the effect of gradually solidifying the fractured earthen site by microorganisms is obvious, and a new idea is provided for solidifying the fractured earthen site by the MICP technology.
Drawings
FIG. 1 is a graph showing the effect of the test of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1
A method for solidifying a soil ruins crack by microorganism grouting specifically comprises the following steps:
1) adding 0.3g of urea analytically pure into 10ml of bacterial liquid cultured for 20 hours in a stationary phase for catalyzing for 15min for later use;
2) sealing and coating the cracks of the earthen site sample with the crack opening degree of 0.5mm, reserving a grouting port at the top of the earthen site sample and above the cracks, and reserving a grout outlet at the bottom of the earthen site sample and below the cracks;
3) stably injecting the bacteria liquid catalyzed in the step 1) into the cracks of the earthen site sample through a grouting port at the flow rate of 3ml/min until the injected bacteria liquid flows out of a grout outlet, and stopping injection;
4) CaCl with the concentration of 0.5mol/L is stably injected through a grouting port at the flow rate of 3ml/min2Solution of CaCl2The solution enters into the cracks of the earthen site sample until CaCl2The solution flows out from the grout outlet, stops injecting, and stands for 30min to ensure that the bacteria liquid and CaCl in the crack2Fully mineralizing the solution;
5) repeating the steps 3) -4) for 8 times.
The samples were subjected to a direct shear test and measured to have a yield strength at the cured interface layer of 79.2kPa at a normal stress of 50kPa, which is a 244% increase over the uncured yield strength.
Example 2
A method for solidifying a soil ruins crack by microorganism grouting specifically comprises the following steps:
1) adding 0.4g of urea analytically pure into 13ml of bacterial liquid cultured in a stationary phase for 25 hours for catalyzing for 18min for later use;
2) sealing and coating the cracks of the earthen site sample with the crack opening degree of 1.0mm, reserving a grouting port at the top of the earthen site sample and above the cracks, and reserving a grout outlet at the bottom of the earthen site sample and below the cracks;
3) stably injecting the bacteria liquid catalyzed in the step 1) into the cracks of the earthen site sample through a grouting port at the flow rate of 4ml/min until the injected bacteria liquid flows out of a grout outlet, and stopping injection;
4) CaCl with the concentration of 1mol/L is stably injected through a grouting port at the flow rate of 4ml/min2Solution of CaCl2The solution enters into the cracks of the earthen site sample until CaCl2The solution flows out from the grout outlet, stops injecting, and stands for 50min to ensure that the bacteria liquid and CaCl in the crack2Fully mineralizing the solution;
5) repeating the steps 3) -4) 9 times.
The samples were subjected to a direct shear test and measured to have a yield strength at the cured interface layer of 61.3kPa at a normal stress of 50kPa, which is a 166% improvement over the uncured yield strength.
Example 3
A method for solidifying a soil ruins crack by microorganism grouting specifically comprises the following steps:
1) adding 0.45g of urea analytically pure into 15ml of bacterial liquid cultured for 30 hours in a stationary phase for catalyzing for 20min for later use;
2) sealing and coating the cracks of the earthen site sample with the crack opening degree of 1.2mm, reserving a grouting port at the top of the earthen site sample and above the cracks, and reserving a grout outlet at the bottom of the earthen site sample and below the cracks;
3) stably injecting the bacteria liquid catalyzed in the step 1) into the cracks of the earthen site sample through a grouting port at the flow rate of 5ml/min until the injected bacteria liquid flows out of a grout outlet, and stopping injection;
4) CaCl with the concentration of 1.5mol/L is stably injected through a grouting opening at the flow rate of 5ml/min2Solution of CaCl2The solution enters into the cracks of the earthen site sample until CaCl2The solution flows out from the grout outlet, stops injecting, and stands for 1h to ensure that the bacteria liquid and CaCl in the crack2Fully mineralizing the solution;
5) repeating the steps 3) -4) for 10 times.
The samples were subjected to a direct shear test and found to have a yield strength at the cured interface layer of 40.9kPa, a 109% increase over the uncured yield strength, at a normal stress of 50 kPa.
Referring to fig. 1, the earthen site test blocks after microorganism grouting and solidification in examples 1-3 are respectively shown from left to right; tests show that the method for solidifying the cracks of the earthen site through microbial grouting has an obvious solidification crack effect, and meanwhile, the solidification strength is influenced by the crack opening degree, the smaller the crack is, the better the solidification effect is, so that the method is particularly important for early expansion and protection of the earthen site.
Claims (7)
1. A method for solidifying soil relic cracks by microorganism grouting is characterized by comprising the following steps: the method specifically comprises the following steps:
1) adding 0.3g-0.45g of urea analytically pure into 10ml-15ml of bacterial liquid cultured for 20-30h in stationary phase for catalyzing for 15min-20min for later use;
2) sealing and coating the cracks of the earthen site sample within the crack opening degree of 1.2mm, reserving a grouting port at the top of the earthen site sample and above the cracks, and reserving a grout outlet at the bottom of the earthen site sample and below the cracks;
3) stably injecting the bacteria liquid catalyzed in the step 1) through a grouting port at the flow rate of 3-5 ml/min, enabling the bacteria liquid to enter cracks of the earthen site sample until the injected bacteria liquid flows out of a grout outlet, and stopping injection;
4) at a flow rate of 3ml/min-5ml/min, CaCl with a concentration of 0.5-1.5mol/L is stably injected through a grouting port2Solution of CaCl2The solution enters into the cracks of the earthen site sample until CaCl2The solution flows out from the grout outlet, stops injecting, and stands for 30min-1h to ensure that the bacteria liquid and CaCl in the crack2Fully mineralizing the solution;
5) repeating the steps 3) -4) for 8-10 times.
2. The method for solidifying the earthen site fractures by microbial grouting according to claim 1, wherein the method comprises the following steps: and 2) coating sealing daub on two sides of the crack for sealing.
3. The method for solidifying the earthen site fractures by microbial grouting according to claim 1, wherein the method comprises the following steps: the flow rates in steps 3) to 4) are adjusted and controlled by a peristaltic pump.
4. The method for solidifying the earthen site fractures by microbial grouting according to claim 1, wherein the method comprises the following steps: the urease activity of the bacterial liquid obtained in the step 3) is 1.0-1.5.
5. The method for solidifying the earthen site fractures by microbial grouting according to claim 1, wherein the method comprises the following steps:
1) adding 0.3g of urea analytically pure into 10ml of bacterial liquid cultured for 20 hours in a stationary phase for catalyzing for 15min for later use;
2) sealing and coating the cracks of the earthen site sample with the crack opening degree of 0.5mm, reserving a grouting port at the top of the earthen site sample and above the cracks, and reserving a grout outlet at the bottom of the earthen site sample and below the cracks;
3) stably injecting the bacteria liquid catalyzed in the step 1) into the cracks of the earthen site sample through a grouting port at the flow rate of 3ml/min until the injected bacteria liquid flows out of a grout outlet, and stopping injection;
4) CaCl with the concentration of 0.5mol/L is stably injected through a grouting port at the flow rate of 3ml/min2Solution of CaCl2The solution enters into the cracks of the earthen site sample until CaCl2The solution flows out from the grout outlet, stops injecting, and stands for 30min to ensure that the bacteria liquid and CaCl in the crack2Fully mineralizing the solution;
5) repeating the steps 3) -4) for 8 times.
6. The method for solidifying the earthen site fractures by microbial grouting according to claim 1, wherein the method comprises the following steps:
1) adding 0.4g of urea analytically pure into 13ml of bacterial liquid cultured in a stationary phase for 25 hours for catalyzing for 18min for later use;
2) sealing and coating the cracks of the earthen site sample with the crack opening degree of 1.0mm, reserving a grouting port at the top of the earthen site sample and above the cracks, and reserving a grout outlet at the bottom of the earthen site sample and below the cracks;
3) stably injecting the bacteria liquid catalyzed in the step 1) into the cracks of the earthen site sample through a grouting port at the flow rate of 4ml/min until the injected bacteria liquid flows out of a grout outlet, and stopping injection;
4) CaCl with the concentration of 1mol/L is stably injected through a grouting port at the flow rate of 4ml/min2Solution of CaCl2The solution enters into the cracks of the earthen site sample until CaCl2The solution flows out from the grout outlet, stops injecting, and stands for 50min to ensure that the bacteria liquid and CaCl in the crack2Fully mineralizing the solution;
5) repeating the steps 3) -4) 9 times.
7. The method for solidifying the earthen site fractures by microbial grouting according to claim 1, wherein the method comprises the following steps:
1) adding 0.45g of urea analytically pure into 15ml of bacterial liquid cultured for 30 hours in a stationary phase for catalyzing for 20min for later use;
2) sealing and coating the cracks of the earthen site sample with the crack opening degree of 1.2mm, reserving a grouting port at the top of the earthen site sample and above the cracks, and reserving a grout outlet at the bottom of the earthen site sample and below the cracks;
3) stably injecting the bacteria liquid catalyzed in the step 1) into the cracks of the earthen site sample through a grouting port at the flow rate of 5ml/min until the injected bacteria liquid flows out of a grout outlet, and stopping injection;
4) CaCl with the concentration of 1.5mol/L is stably injected through a grouting opening at the flow rate of 5ml/min2Solution of CaCl2The solution enters into the cracks of the earthen site sample until CaCl2The solution flows out from the grout outlet, stops injecting, and stands for 1h to ensure that the bacteria liquid and CaCl in the crack2Fully mineralizing the solution;
5) repeating the steps 3) -4) for 10 times.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114197436A (en) * | 2022-01-26 | 2022-03-18 | 南京大学 | Continuous microbe layered mineralization repairing method for rock mass steep non-through micro-crack |
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2021
- 2021-06-10 CN CN202110649478.7A patent/CN113235567A/en active Pending
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| US20140377014A1 (en) * | 2012-01-16 | 2014-12-25 | Solentanche Freyssinet | Method for consolidating soil |
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| CN104818719A (en) * | 2015-03-16 | 2015-08-05 | 山东建筑大学 | Microorganism grouting method capable of improving early-stage calcium carbonate deposit volume |
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| CN114197436A (en) * | 2022-01-26 | 2022-03-18 | 南京大学 | Continuous microbe layered mineralization repairing method for rock mass steep non-through micro-crack |
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