CN104402287B - A kind of method for refinforced cement sill alkali burnout resistance energy - Google Patents

A kind of method for refinforced cement sill alkali burnout resistance energy Download PDF

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CN104402287B
CN104402287B CN201410610689.XA CN201410610689A CN104402287B CN 104402287 B CN104402287 B CN 104402287B CN 201410610689 A CN201410610689 A CN 201410610689A CN 104402287 B CN104402287 B CN 104402287B
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cement
bacillus cereus
colloid bacillus
bacterium liquid
based material
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CN104402287A (en
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钱春香
曹天骥
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Southeast University
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Abstract

The invention discloses a kind of method for refinforced cement sill alkali burnout resistance energy, utilize carbonic anhydrase microorganism to produce carbonic anhydrase and catch CO 2, promote CO 2be converted into CO 3 2-, the inner Soluble Ca (OH) of accelerated material 2be converted into CaCO 3deposition.Compared with the method for the anti-accumulation of salt in the surface soil ability of other refinforced cement sills, there is pollution-free, the simple advantage of technique.Prepare colloid bacillus cereus after the steps include: that colloid bacillus cereus is seeded to culture medium culturing and concentrate bacterium liquid, add when shaping cement-based material in mixing water and make it be evenly distributed on material internal.Carry out alkali burnout resistance to test specimen can test, result shows, the surperficial accumulation of salt in the surface soil area of cement-based material significantly reduces, and anti-accumulation of salt in the surface soil ability is significantly improved.

Description

A kind of method for refinforced cement sill alkali burnout resistance energy
Technical field
The present invention relates to the interdisciplinary science technology of microbiological art and building material field, be specifically related to a kind of method of alkali burnout resistance performance of refinforced cement sill.
Background technology
Cement-based material is the material of construction be most widely used at present, outside the building has large-tonnage product in bilge construction, as: cement-based finishing mortar, precast wall panel etc.But cement-based material is under the invasion and attack of the factors such as outside rainwater, very easily there is accumulation of salt in the surface soil phenomenon in surface.Accumulation of salt in the surface soil phenomenon mostly is the inner Ca (OH) of cement-based material 2caused by water migration to material surface deposition, show as material surface and occur " bloom ", have a strong impact on the aesthetic properties of material.The main method of current raising cement-based material alkali burnout resistance energy has Ca (OH) in volcanic ash raw materials such as adding metakaolin and material 2reaction, material are carried out water repellent treatment and are reduced the method such as Water Transport, and these methods all exist that cost is higher, the shortcoming of complex process.
Colloid bacillus cereus is also known as silicate bacteria, its key property is potassium, the silicon that can decomposite in the mineral such as feldspar, mica, also the phosphorus in phosphatic rock be can decomposite, and plant growth substance and multiple enzyme secreted, to strengthen the resistibility of crop to some diseases.At present, this bacterioid is the best bacterial classification being used as bio-feritlizer, and to promote the conversion of soil inavailable phosphorus potassium, increase the supply of soil phosphorus potassium, improve crop yield, the intensity of its colloid bacillus cereus phosphorus decomposing, potassium decomposing, solution silicon is superpower.Colloid bacillus cereus can produce carbonic anhydrase, has certain effect to the fixing tool of carbonic acid gas, and this characteristic has been applied in the crack microorganism selfreparing of cement-based material.
Summary of the invention
The technical problem solved: for the deficiencies in the prior art, the present invention proposes a kind of method for refinforced cement sill alkali burnout resistance energy, for solving
Technical scheme: for solving the problems of the technologies described above, the present invention by the following technical solutions:
A kind of method for refinforced cement sill alkali burnout resistance energy, carry out in the process of cement-based material making utilizing raw material, when shaping cement-based material, in raw material, add colloid bacillus cereus solution and mix, carrying out blanks and moulding and conserving afterwards and prepare final cement-based material.
The carbonic anhydrase that the present invention utilizes colloid bacillus cereus to produce, and carbonic anhydrase has absorption CO 2be converted to CO 3 2-ability, be embedded in cement-based material, the CO in air can be absorbed 2make the Ca (OH) of solubility in cement-based material 2form precipitation, to external migration, thus the anti-accumulation of salt in the surface soil ability of cement-based material cannot be improved.Compared with the method for the anti-accumulation of salt in the surface soil ability of raising material adopted at present, there is technique simple, the advantage of low-carbon environment-friendly.
Further, in the present invention, the method specifically comprises the steps:
Step (1), be seeded in culture medium culturing by colloid bacillus cereus, preparing cell concentration is 10 6~ 10 7the colloid bacillus cereus of individual/mL concentrates bacterium liquid;
Step (2), raw material through process become the gelling material being in gelatinized, and before the preparation of cement-based material proceeds to coagulation forming maintenance operation, colloid bacillus cereus being concentrated bacterium liquid, to add pre-dilution in mixing water even, after making cement-based material setting and harden, colloid bacillus cereus can be evenly distributed in material internal, wherein, colloid bacillus cereus concentrate that the quality of bacterium liquid is gelling material quality in the raw material preparing refinforced cement sill 3% ~ 10%.
Colloid bacillus, in the process of setting and hardening together with cement-based material, fully absorbs the CO in air 2with the Soluble Ca (OH) in cement-based material 2produce chemical reaction, generate insoluble CaCO 3, so Soluble Ca (OH) in the test specimen finally made 2greatly reduce, alkali burnout resistance can be improved.
As preferably, in the present invention, colloid bacillus cereus concentrate that the quality of bacterium liquid is gelling material quality in the raw material preparing refinforced cement sill 6%.Through experiment test, best results when 6%.
Beneficial effect:
1, compared with the mineral material that with traditional interpolation, there is pozzolanic activity, have employed interpolation in a creative way and there is absorption CO 2the method of ability microflora, accelerates the CO in invert atmosphere 2gas reforming is CO 3 2-with material internal Ca (OH) 2reaction, generates insoluble CaCO 3precipitation, decreases Ca (OH) 2with Water Transport to the probability on cement-based material surface, thus the alkali burnout resistance energy of strongthener.
2, traditional interpolation pozzolanic activity mineral material is for material internal Ca (OH) 2the reduction of content is disposable, and mineral have been consumed rear reaction and have namely stopped, and microflora is for CO in material 2with Ca (OH) 2reaction is equivalent to catalyzer, has all the time and reduce Ca (OH) in material before the non-death of microorganism 2the effect of content, has the incomparable advantage of mineral adding material for there being the cement-based material of inner low alkalinity requirement.
3, microbial preparation produces features such as having efficient, environmental protection, compared with non-renewable mineral wealth, meet the requirement of current Sustainable development, and microorganism can absorb greenhouse gases CO 2, meet the trend that " low-carbon (LC) " is economic.
Accompanying drawing explanation
Fig. 1 is under the same terms, CO 2gas passes into saturated Ca (OH) respectively 2solution and the saturated Ca (OH) adding bacterium liquid 2cO in solution 2uptake rate curve.
Fig. 2 is cement paste test group and with the addition of bacterium liquid test group test specimen thermogravimetric analysis figure after 7 days.
Fig. 3 is that cement-based material alkali burnout resistance can test schematic diagram.
For cement paste test group tests the material surface image after anti-accumulation of salt in the surface soil ability on the left of Fig. 4; The material surface image after anti-accumulation of salt in the surface soil ability is tested for adding bacterium liquid group on the right side of Fig. 4.
Fig. 5 is blank group, adds bacterium liquid group material surface accumulation of salt in the surface soil degree and accumulation of salt in the surface soil area.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further described.
Buy from Chinese Research for Industrial Microbial Germ preservation center and obtain the colloid bacillus cereus (Bacillusmucilaginous) being numbered 21698.
First experimental demonstration colloid bacillus cereus absorbs CO 2ability.
Prepare two parts of Ca (OH) 2solution, a copy of it is saturated Ca (OH) 2solution, a for adding the saturated Ca (OH) of colloid bacillus cereus bacterium liquid in addition 2solution.By CO 2gas passes in these two parts of solution respectively, draws CO 2as shown in Figure 1, CA represents colloid bacillus cereus to uptake rate curve.The visible saturated Ca (OH) adding colloid bacillus cereus bacterium liquid 2cO in solution 2uptake rate all the time higher than another part of solution.Prove that colloid bacillus cereus is to absorption CO 2truly have obvious effect.
Below in order to contrast the validity proving the inventive method, step is tested in accordance with the following methods:
Embodiment 1
(1) the concentrated bacterium liquid of colloid bacillus cereus (Bacillusmucilaginous) is obtained: colloid bacillus cereus is inoculated in the culture medium solution after sterilizing, often liter of substratum contains sucrose 8 ~ 12g, Na 2hPO 412H 2o2 ~ 3g, MgSO 40.4 ~ 0.6g, CaCO 30.5 ~ 1.5g, KCl0.1 ~ 0.2g, (NH 4) 2sO 40.4 ~ 0.6g, yeast extract 0.2 ~ 0.4g, and control pH is 7 ~ 8, shaking culture 24h at 30 ~ 37 DEG C, obtain the bacterium liquid containing colloid bacillus cereus, at 4 DEG C after 6000 ~ 8000rpm high speed centrifugation, 10 ~ 15min, add deionized water after the substratum nutritive substance on removing upper strata, in final obtained concentrated bacterium liquid, contained cell concentration is 10 6~ 10 7individual/mL.
(2) shaping cement-based material: the raw material of shaping cement-based material becomes through process the gelling material being in gelatinized, get the mixing water of the quality such as the concentrated bacterium liquid replacement of 6% gelling material quality, concentrated bacterium liquid is diluted in remaining mixing water and carries out cement-based material coagulation forming, make colloid bacillus cereus can be evenly distributed in cement-based material inside; Meanwhile, the shaping same proportioning cement-based material not adding concentrated bacterium liquid in the lump;
Under standard mortar normal curing condition, maintenance is after 7 days, the inner Ca (OH) of the cement-based material after utilizing thermogravimetric analysis shaping 2content, obtain thermogravimetric analysis figure as shown in Figure 2, test shows: through heating, and the cement-based material mass loss ground adding colloid bacillus cereus bacterium liquid is not more, reflects its originally inner Ca (OH) 2content is higher, obtains, add the Ca (OH) of the cement-based material inside after concentrated bacterium liquid after concrete quantification 2compare the Ca (OH) of the cement-based material inside not adding concentrated bacterium liquid 2content reduces 7.45%.
(3) alkali burnout resistance can be tested: after the test specimen solidification of cement-based material, as shown in Figure 3, with PVC board close test specimen surrounding make material surface can add water formed certain thickness water layer, constant speed air-flow is used to brush water layer surface until evaporate completely after leaving standstill 24h, repeat 3 times, gathering surface of test piece image obtains as shown in Figure 4, naked eyes can find out that the material surface obvious accumulation of salt in the surface soil phenomenon that with the addition of colloid bacillus cereus bacterium liquid is slighter significantly very much, then computer image analysis is carried out to image, obtain quantification figure as shown in Figure 5, as seen from Figure 5, the material surface that with the addition of colloid bacillus cereus bacterium liquid is the relevant range that the area in slight accumulation of salt in the surface soil region or severe accumulation of salt in the surface soil region is all less than the surface of the material not adding colloid bacillus cereus bacterium liquid.Therefore can conclusion: after adding colloid bacillus cereus bacterium liquid, material surface accumulation of salt in the surface soil degree and area significantly reduce, and alkali burnout resistance can significantly promote.
The data for reference of following similar experiment is several times provided simultaneously:
Embodiment 2:
(1) sucrose 12g, Na is taken 2hPO 412H 2o3g, MgSO 40.6g, CaCO 31.5g, KCl0.2g, (NH 4) 2sO 40.6g, yeast extract 0.4g dissolve in 1000mL deionized water, be configured to required culture medium solution, pH is regulated to be 7, under 125 DEG C of high temperature, sterilizing is taken out to be cooled after 25 minutes, colloid bacillus cereus is seeded in cooling culture medium solution, shaking culture at 30 DEG C, oscillation frequency is 170r/min, incubation time 24h;
(2) by cultured concentrated bacterium liquid high speed centrifugation 10min, centrifuge speed is 8000rpm, and temperature is 4 DEG C, removes upper strata substratum nutritive substance, adds deionized water 100mL and make concentrated bacterium liquid;
(3) shaping cement-based material water-cement ratio W/C=0.3076, gel material content 1300g, sand coarse aggregate ratio is 1, red iron oxide dyestuff quality is gelling material 5%, adopt the thermal insulation mortar die trial of 300mm*300mm*30mm shaping, one group of test specimen does not add concentrated bacterium liquid and directly uses mixing water mix, and the mixing water of last group of equal in quality got by another group test specimen, but needing to concentrate after bacterium liquid replaces the 130g mixing water of its fair average quality with the common 130g of gel material content 10% carries out mix;
(4) be placed on until test specimen solidification and carry out alkali burnout resistance after maintenance 7d under standard mortar normal curing condition and can test.
After 2 accumulation of salt in the surface soil process, the area not adding the test specimen serious accumulation of salt in the surface soil region of bacterium liquid accounts for 84.17% of the whole surface of test specimen, and the area adding the serious accumulation of salt in the surface soil region of test specimen of bacterium liquid is only 43.96%, and anti-accumulation of salt in the surface soil ability has remarkable lifting.
Embodiment 3:
(1) sucrose 9.6g, Na is taken 2hPO 412H 2o2.4g, MgSO 40.48g, CaCO 31.2g, KCl0.16g, (NH 4) 2sO 40.48g, yeast extract 0.32g dissolve in 800mL deionized water, be configured to required culture medium solution, pH is regulated to be 7, under 125 DEG C of high temperature, sterilizing is taken out to be cooled after 25 minutes, colloid bacillus cereus is seeded in cooling culture medium solution, shaking culture at 30 DEG C, oscillation frequency is 170r/min, incubation time 24h;
(2) by cultured bacterium liquid high speed centrifugation 10min, centrifuge speed is 8000rpm, and temperature is 4 DEG C, removes upper strata substratum nutritive substance, adds deionized water 80mL and make concentrated bacterium liquid;
(3) shaping cement-based material water-cement ratio W/C=0.2576, gel material content 1200g, black iron oxide dyestuff quality is gelling material 7%, adopt the thermal insulation mortar die trial of 300mm*300mm*30mm shaping, the mixing water concentrating the quality such as bacterium liquid replacement with the common 36g of gel material content 3% carries out mix;
(4) be placed on until test specimen solidification and carry out alkali burnout resistance after maintenance 7d under standard mortar normal curing condition and can test.
After 3 accumulation of salt in the surface soil process, the area in the serious accumulation of salt in the surface soil region of test specimen is 26.30%, has good alkali burnout resistance energy.
Embodiment 4:
(1) sucrose 8g, Na is taken 2hPO 412H 2o2g, MgSO 40.4g, CaCO 30.5g, KCl0.1g, (NH 4) 2sO 40.4g, yeast extract 0.2g dissolve in 500mL deionized water, be configured to required culture medium solution, pH is regulated to be 7, under 125 DEG C of high temperature, sterilizing is taken out to be cooled after 25 minutes, colloid bacillus cereus is seeded in cooling culture medium solution, shaking culture at 30 DEG C, oscillation frequency is 170r/min, incubation time 24h;
(2) by cultured bacterium liquid high speed centrifugation 10min, centrifuge speed is 8000rpm, and temperature is 4 DEG C, removes upper strata substratum nutritive substance, adds deionized water 50mL and make concentrated bacterium liquid;
(3) shaping cement-based material water-cement ratio W/C=0.3076, adopt the thermal insulation mortar die trial of 300mm*300mm*30mm shaping, test specimen is divided into bilayer structure, ash concrete and white cement is used to be major gelled material respectively, sand coarse aggregate ratio is 1, first group of test specimen does not add bacterium liquid, and it is shaping that second group of test specimen substitutes mixing water with the bacterium liquid of 8% gelling material quality;
(4) be placed on until test specimen solidification and carry out alkali burnout resistance after maintenance 7d under standard mortar normal curing condition and can test.
Test shows: the area not adding the serious accumulation of salt in the surface soil region of test specimen of bacterium liquid is 54.05%, and the serious accumulation of salt in the surface soil region area of test specimen adding bacterium liquid is only 24.33%, and anti-accumulation of salt in the surface soil ability has a distinct increment.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (2)

1. the method for refinforced cement sill alkali burnout resistance energy, it is characterized in that: carry out in the process of cement-based material making utilizing raw material, when shaping cement-based material, in raw material, add colloid bacillus cereus solution and mix, carrying out blanks and moulding and conserving afterwards and prepare final cement-based material; The method specifically comprises the steps:
Step (1), colloid bacillus cereus is seeded to culture medium culturing, preparing cell concentration is 10 6~ 10 7the colloid bacillus cereus of individual/mL concentrates bacterium liquid;
Before the preparation of step (2), cement-based material proceeds to coagulation forming maintenance operation, colloid bacillus cereus being concentrated bacterium liquid, to add pre-dilution in mixing water even, after making cement-based material setting and harden, colloid bacillus cereus can be evenly distributed in material internal, wherein, colloid bacillus cereus concentrate that the quality of bacterium liquid is gelling material quality in the raw material preparing refinforced cement sill 3% ~ 10%.
2. the method for refinforced cement sill alkali burnout resistance energy according to claim 1, it is characterized in that: in step (2), the quality that colloid bacillus cereus concentrates bacterium liquid is 6% of gelling material quality in the raw material preparing refinforced cement sill.
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CN111362722A (en) * 2019-10-31 2020-07-03 东南大学 Method for regulating and controlling gradient mineralization of cement-based material
CN111978026B (en) * 2020-09-04 2021-08-27 中国矿业大学(北京) Mineralized and sealed CO2Mining microbial cementing filling material and preparation method thereof
CN114853398A (en) * 2022-06-08 2022-08-05 东南大学 Saltpetering-resistant stain-resistant self-cleaning decorative cement-based product and preparation method thereof

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