CN110330251A - Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising - Google Patents
Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising Download PDFInfo
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- CN110330251A CN110330251A CN201910638411.6A CN201910638411A CN110330251A CN 110330251 A CN110330251 A CN 110330251A CN 201910638411 A CN201910638411 A CN 201910638411A CN 110330251 A CN110330251 A CN 110330251A
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- calcareous sand
- mineralising
- reinforces
- bacillus alcaligenes
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- 239000004576 sand Substances 0.000 title claims abstract description 70
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 37
- 241000588986 Alcaligenes Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 18
- 238000005728 strengthening Methods 0.000 title claims abstract description 18
- 230000003014 reinforcing effect Effects 0.000 claims abstract description 20
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims abstract description 14
- 230000008569 process Effects 0.000 claims abstract description 9
- 239000011259 mixed solution Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 10
- 159000000007 calcium salts Chemical class 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 6
- 230000002572 peristaltic effect Effects 0.000 claims description 6
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 2
- 239000001527 calcium lactate Substances 0.000 claims description 2
- 229960002401 calcium lactate Drugs 0.000 claims description 2
- 235000011086 calcium lactate Nutrition 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 15
- 238000007596 consolidation process Methods 0.000 abstract description 15
- 230000000694 effects Effects 0.000 abstract description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 11
- 244000005700 microbiome Species 0.000 abstract description 9
- 229910021529 ammonia Inorganic materials 0.000 abstract description 8
- 239000004568 cement Substances 0.000 abstract description 8
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 abstract description 6
- 238000001764 infiltration Methods 0.000 abstract description 6
- 230000008595 infiltration Effects 0.000 abstract description 6
- 241000894006 Bacteria Species 0.000 abstract description 5
- 239000011398 Portland cement Substances 0.000 abstract description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract description 5
- 239000004202 carbamide Substances 0.000 abstract description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 4
- 230000007062 hydrolysis Effects 0.000 abstract description 3
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 3
- 238000011160 research Methods 0.000 abstract description 3
- 102000005701 Calcium-Binding Proteins Human genes 0.000 abstract description 2
- 108010045403 Calcium-Binding Proteins Proteins 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 3
- 239000002803 fossil fuel Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 235000005979 Citrus limon Nutrition 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- 241000193395 Sporosarcina pasteurii Species 0.000 description 2
- 238000002441 X-ray diffraction Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000000292 calcium oxide Substances 0.000 description 2
- 235000012255 calcium oxide Nutrition 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000002787 reinforcement Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 244000248349 Citrus limon Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000010304 firing Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000003469 silicate cement Substances 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B20/00—Use of materials as fillers for mortars, concrete or artificial stone according to more than one of groups C04B14/00 - C04B18/00 and characterised by shape or grain distribution; Treatment of materials according to more than one of the groups C04B14/00 - C04B18/00 specially adapted to enhance their filling properties in mortars, concrete or artificial stone; Expanding or defibrillating materials
- C04B20/02—Treatment
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B40/00—Processes, in general, for influencing or modifying the properties of mortars, concrete or artificial stone compositions, e.g. their setting or hardening ability
- C04B40/0028—Aspects relating to the mixing step of the mortar preparation
- C04B40/0039—Premixtures of ingredients
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Ceramic Engineering (AREA)
- Materials Engineering (AREA)
- Structural Engineering (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of Bacillus alcaligenes to reinforce the purposes in calcareous sand technique as strengthening agent in mineralising.The present invention is found surprisingly that by research, Bacillus alcaligenes is added to mineralising as strengthening agent to reinforce in the grouting process of calcareous sand, on the one hand, compared with existing urase bacteria microorganism cement generates ammonia in hydrolysis urea process, citrate can be hydrolyzed to carbonate, carbonate therein and calcium binding generate calcium carbonate, and do not generate ammonia in mineralising consolidation process, effect on environment is smaller, more environmentally protective；On the other hand, compared with common portland cement, have bigger infiltration coefficient, can the more preferable draining and infiltration that must guarantee ground while reinforcing side slope and ground, to widen the application range of Bacillus alcaligenes in the market significantly.
Description
Technical field
The present invention relates to the new application of Bacillus alcaligenes, in particular to Bacillus alcaligenes reinforces conduct in calcareous sand technique in mineralising
The purposes of strengthening agent
Background technique
Portland cement is gel rubber material the most commonly used in architectural engineering, in the historical progress and development from now on of the mankind
In all occupy important position.But the production process of portland cement needs a large amount of heat to convert ripe stone for quick lime
Ash, under existing productivity level, the heat of this part is generated by the burning of fossil fuel.So, silicic acid
Need to consume a large amount of fossil fuel in the production process of salt cement, and during combustion of fossil fuels and firing quick lime
A large amount of carbon dioxide (CO will be discharged2), CO in atmosphere2The promotion of concentration will cause " greenhouse effects ".In addition, silicate cement
Various pernicious gases can be also generated in mud production process, and ecological environment is adversely affected.
Microorganism cement belongs to biogel material, and cardinal principle is the hydrolysis using microorganism, generates CO3 2-
Or NH4 +Plasma, with loose material Ca as contained in sand2+Etc. sediment is formed, loose flocculation is got up,
Play the role of cured.The gel rubber material traditional compared to portland cement etc., its production of biogel material and mechanism are equal
It is more environmentally protective.In addition, the mechanism of biogel material can also be carried out carefully by controlling the growing environment of microorganism
The regulation of cause, to realize the automation building course of " intelligence " and " controllable ".Microorganism cement is in side slope and foundation stabilization side
Mask has wide practical use, and belongs to the research hotspot of civil engineering and bioengineering field in recent years.
But mainly using urase bacterium (Bacillus pasteurii) in common microorganism cement, the urase bacterium is micro-
Biology generates urase, under the action of urase, urea can fast hydrolyzing generate ammonia and carbonate, carbonate can and solution in
Calcium ion effect, generates calcium carbonate, forms gel, and loose sand grains is solidified.But there are also significant component of ammonia due to
As reaction carries out solution ph raising, it can not dissolve and be directly discharged in air, have a negative impact to environment.Therefore,
Ammonia emission of the existing microorganism cement material in mineralising consolidation is solved, really realizes the microorganism cement tool of green non-pollution
There is highly important practical significance.
Summary of the invention
It is reinforced in calcareous sand technique to solve the above problems, the purpose of the present invention is to provide a kind of Bacillus alcaligenes in mineralising
Purposes as strengthening agent.
Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising.
Wherein, the bacterial concentration OD of the Bacillus alcaligenes600=1.3-1.6, number GIM1.754, Latin literary fame are
Alcaligenessp, other numbers are ACCC 01730, are bought in Guangdong Province's Organism Depositary.
Wherein, it is grouting process that the mineralising, which reinforces calcareous sand technique,.
Preferably, the grouting process, includes the following steps:
1) citrate is added in Bacillus alcaligenes solution, forms mixing liquid；
2) above-mentioned mixed solution peristaltic pump is injected in calcareous sand column, stands 1-3 hours；
3) again with peristaltic pump to the calcium salt soln of above-mentioned calcareous sand column injection 100mL, 5-7 hours are stood, by calcareous sand column
In surplus solution discharge；
4) step 2) -3 is repeated), until it can not reinject mixing liquid into above-mentioned calcareous sand column；
5) the calcareous sand column good to above-mentioned reinforcing carries out drying maintenance demoulding.
Wherein, in step 1), the molarity of the citrate is 0.1-1mol/l.
Wherein, in step 1), the volume ratio of Bacillus alcaligenes and citrate is 0.5-1.5:1 in the mixed solution.
Wherein, in step 2), the dosage of the mixed solution is 50-150mL.
Wherein, in step 2), the size of the calcareous sand column is Ф 5cm × 10cm.
Wherein, in step 3), the calcium salt soln is selected from CaCl2One or more of solution, calcium nitrate, calcium lactate.
Wherein, in step 3), the molarity of the calcium salt soln is 0.1-1mol/l, volume 50-150mL.
Compared with prior art, the present invention having the following beneficial effects:
The present invention is found surprisingly that by research, is added to the filling that mineralising reinforces calcareous sand for Bacillus alcaligenes as strengthening agent
In sizing process, on the one hand, can be by lemon compared with existing urase bacteria microorganism cement generates ammonia in hydrolysis urea process
Lemon hydrochlorate is hydrolyzed to carbonate, and carbonate therein and calcium binding generate calcium carbonate, and do not produce in mineralising consolidation process
Ammonifying gas, effect on environment is smaller, more environmentally protective；On the other hand, compared with common portland cement, have bigger
Infiltration coefficient, can the more preferable draining and infiltration that must guarantee ground while reinforcing side slope and ground, to widen significantly
The application range of Bacillus alcaligenes in the market.
Detailed description of the invention
Fig. 1 is the calcareous sand column schematic diagram of Φ 5cm × 10cm used by embodiment 1-3 and comparative example 1-4 reinforcement experiment；
Fig. 2 is the result figure for obtain after XRD analysis to the loose calcareous sand column of embodiment 1；
Fig. 3-7 is the scanning electron microscope (SEM) photograph of the calcareous sand column after the consolidation by grouting under the different amplification factor of embodiment 1.
Specific embodiment
Below in conjunction with attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that institute
The embodiment of description is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the implementation in the present invention
Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to
The scope of protection of the invention.
In the embodiment of the present invention, raw material used in the present invention is as follows:
The bacterial concentration OD of Bacillus alcaligenes600=1.3-1.6, number are GIM 1.754, and Latin literary fame is
Alcaligenessp, other numbers are ACCC 01730, are bought in Guangdong Province's Organism Depositary；
Citrate, calcium salt soln can be commercially available by commercially available.
The test method of each performance indicator:
Uniaxial compressive strength: after the completion of consolidation by grouting, Example 1-3 and comparative example 1-4 dry the sand column conserved,
Uniaxial compressive test is carried out, its uniaxial compressive strength is tested；
Infiltration coefficient: the calcareous sand column after Example 1-3 and comparative example 1-4 reinforcing carries out permeability test, tests its infiltration
Saturating coefficient；
Whether the test method of ammonia: GB/T 14679-1993 air quality-ammonia measurement-sodium hypochlorite-bigcatkin willow is generated
Sour spectrophotometry.
Reinforce the mineralogical composition in the sand column of front and back: to the calcareous sand column before reinforcing and the calcareous sand after the reinforcing of embodiment 1
Column carries out XRD detection, and the mineralogical composition in the sand column of front and back is reinforced in detection；
It reinforces front and back calcareous sand column and surrounds and watches structure: to calcareous sand column before reinforcing and calcareous after the reinforcing of embodiment 1
The mode that scanning electron microscope is respectively adopted in sand column is scanned calcareous sand column, and calcareous sand column surrounds and watches structure before and after observation is reinforced.
Mineralising containing Bacillus alcaligenes reinforces calcareous sand grouting process:
Embodiment 1
1) citrate that molarity is 0.8mol/l is added to strain concentration OD600=1.5 Bacillus alcaligenes
In solution, mixing liquid is formed, wherein the volume ratio of Bacillus alcaligenes and citrate is 1:1 in the mixed solution；
2) the above-mentioned mixed solution of 100mL is injected in the calcareous sand column of Ф 5cm × 10cm with peristaltic pump, stands 2 hours；
3) CaCl for being again 0.8mol/l with the molarity of from peristaltic pump to above-mentioned calcareous sand column injection 100mL2It is molten
Liquid stands 6 hours, and the surplus solution in calcareous sand column is discharged；
4) step 2) -3 is repeated), until it can not reinject mixing liquid into above-mentioned calcareous sand column；
5) the calcareous sand column good to above-mentioned reinforcing carries out drying maintenance demoulding.Calcareous sand column after resulting reinforcing
Consolidation effect
The calcareous sand column schematic diagram of Φ 5cm × 10cm used by the reinforcement experiment of calcareous sand column after resulting reinforcing is such as
Shown in Fig. 1；The result obtained after XRD analysis is carried out to the calcareous sand column of the calcareous sand column after resulting reinforcing as shown in Fig. 2, figure
In be shown in consolidation by grouting after, have in calcareous sand calcium carbonate (85-0849) generation, and the ingredient of calcareous sand be Aragonite
(99-0013)；Calcareous sand column of the calcareous sand column after the consolidation by grouting under different amplification factors after resulting reinforcing is swept
Retouch electron microscope as shown in fig. 3 to 7；The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Embodiment 2
The volume ratio of Bacillus alcaligenes and citrate is 0.5:1 in the step 1) mixed solution, other are the same as embodiment 1.
The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Embodiment 3
The volume ratio of Bacillus alcaligenes and citrate is 1.5:1 in the step 1) mixed solution, other are the same as embodiment 1.
The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Comparative example 1
The volume ratio or molar ratio of Bacillus alcaligenes and citrate are 0.1:1 in the step 1) mixed solution, other are same
Embodiment 1.The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Comparative example 2
The volume ratio or molar ratio of Bacillus alcaligenes and citrate are 2:1 in the step 1) mixed solution, other are the same as real
Apply example 1.The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Comparative example 3
Bacillus alcaligenes is replaced using urase bacterium, while adding the urea of 0.1mol/l, other are the same as embodiment 1.It is resulting to add
Gu the consolidation effect of calcareous sand column after is as shown in table 1.
Comparative example 4
Bacillus alcaligenes is replaced using Bacillus pasteurii, while adding the urea of 0.1mol/l, other are the same as embodiment 1.Institute
The consolidation effect of calcareous sand column after the reinforcing obtained is as shown in table 1.
Table 1
The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all at this
Under the inventive concept of invention, using equivalent structure transformation made by description of the invention and accompanying drawing content, or directly/use indirectly
It is included in other related technical areas in scope of patent protection of the invention.
Claims (10)
1. Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising.
2. Bacillus alcaligenes according to claim 1 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special
Sign is, the OD of the Bacillus alcaligenes600=1.3-1.6, number are GIM 1.754, Latin literary fame Alcaligenessp,
It is ACCC 01730 that he, which numbers, is bought in Guangdong Province's Organism Depositary.
3. Bacillus alcaligenes according to claim 1 or 2 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising,
It is characterized in that, it is grouting process that the mineralising, which reinforces calcareous sand technique,.
4. Bacillus alcaligenes according to claim 3 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special
Sign is that the grouting process includes the following steps:
1) citrate is added in Bacillus alcaligenes solution, forms mixing liquid；
2) above-mentioned mixed solution peristaltic pump is injected in calcareous sand column, stands 1-3 hours；
3) calcium salt soln is injected to above-mentioned calcareous sand column with peristaltic pump again, 5-7 hours is stood, by the surplus solution in calcareous sand column
Discharge；
4) step 2-3 is repeated), until it can not reinject mixing liquid into above-mentioned calcareous sand column；
5) the calcareous sand column good to above-mentioned reinforcing carries out drying maintenance demoulding.
5. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special
Sign is, in step 1), the molarity of the citrate is 0.1-1mol/l.
6. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special
Sign is, in step 1), the volume ratio of Bacillus alcaligenes and citrate is 0.5-1.5:1 in the mixed solution.
7. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special
Sign is, in step 2, the dosage of the mixed solution is 50-150mL.
8. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special
Sign is, in step 2, the size of the calcareous sand column is Ф 5 cm × 10 cm.
9. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special
Sign is, in step 3), the calcium salt soln is selected from CaCl2One or more of solution, calcium nitrate, calcium lactate.
10. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special
Sign is, in step 3), the molarity of the calcium salt soln is 0.1-1mol/l, volume 50-150mL.
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| CN201910638411.6A CN110330251A (en) | 2019-07-16 | 2019-07-16 | Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising |
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| CN201910638411.6A CN110330251A (en) | 2019-07-16 | 2019-07-16 | Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110713959A (en) * | 2019-11-28 | 2020-01-21 | 上海海事大学 | Microbial agent for heavy metal pollution remediation and application thereof |
| CN111056782A (en) * | 2019-12-31 | 2020-04-24 | 中交路桥华南工程有限公司 | Microbial self-repairing method for tunnel lining concrete cracks |
| CN113123353A (en) * | 2021-04-16 | 2021-07-16 | 合肥工业大学 | Method for solidifying redbed slope surface soil by microbial preparation |
| CN113666407A (en) * | 2021-08-20 | 2021-11-19 | 中山大学 | Comprehensive utilization process for extracting calcium ions in south sea calcareous sand and treating algae sewage and application thereof |
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| CN101644047A (en) * | 2009-07-23 | 2010-02-10 | 东南大学 | Method of adhering loose sands employing microbial mineralization |
| CN106856700A (en) * | 2016-12-29 | 2017-06-20 | 河海大学 | Desert planting structure and in desert planting plants method |
| WO2018013830A1 (en) * | 2016-07-14 | 2018-01-18 | Dong-Kyun Seo | Antimicrobial geopolymer compositions |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101644047A (en) * | 2009-07-23 | 2010-02-10 | 东南大学 | Method of adhering loose sands employing microbial mineralization |
| WO2018013830A1 (en) * | 2016-07-14 | 2018-01-18 | Dong-Kyun Seo | Antimicrobial geopolymer compositions |
| CN106856700A (en) * | 2016-12-29 | 2017-06-20 | 河海大学 | Desert planting structure and in desert planting plants method |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110713959A (en) * | 2019-11-28 | 2020-01-21 | 上海海事大学 | Microbial agent for heavy metal pollution remediation and application thereof |
| CN111056782A (en) * | 2019-12-31 | 2020-04-24 | 中交路桥华南工程有限公司 | Microbial self-repairing method for tunnel lining concrete cracks |
| CN111056782B (en) * | 2019-12-31 | 2021-07-30 | 中交路桥华南工程有限公司 | Microbial self-repairing method for tunnel lining concrete cracks |
| CN113123353A (en) * | 2021-04-16 | 2021-07-16 | 合肥工业大学 | Method for solidifying redbed slope surface soil by microbial preparation |
| CN113666407A (en) * | 2021-08-20 | 2021-11-19 | 中山大学 | Comprehensive utilization process for extracting calcium ions in south sea calcareous sand and treating algae sewage and application thereof |
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Application publication date: 20191015 |