CN110330251A - Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising - Google Patents

Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising Download PDF

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Publication number
CN110330251A
CN110330251A CN201910638411.6A CN201910638411A CN110330251A CN 110330251 A CN110330251 A CN 110330251A CN 201910638411 A CN201910638411 A CN 201910638411A CN 110330251 A CN110330251 A CN 110330251A
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China
Prior art keywords
calcareous sand
mineralising
reinforces
bacillus alcaligenes
purposes
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CN201910638411.6A
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Inventor
马建军
黄林冲
梁禹
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Sun Yat Sen University
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Sun Yat Sen University
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Priority to CN201910638411.6A priority Critical patent/CN110330251A/en
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    • CCHEMISTRY; METALLURGY
    • C04CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04BLIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B20/00Use of materials as fillers for mortars, concrete or artificial stone according to more than one of groups C04B14/00 - C04B18/00 and characterised by shape or grain distribution; Treatment of materials according to more than one of the groups C04B14/00 - C04B18/00 specially adapted to enhance their filling properties in mortars, concrete or artificial stone; Expanding or defibrillating materials
    • C04B20/02Treatment
    • CCHEMISTRY; METALLURGY
    • C04CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04BLIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B40/00Processes, in general, for influencing or modifying the properties of mortars, concrete or artificial stone compositions, e.g. their setting or hardening ability
    • C04B40/0028Aspects relating to the mixing step of the mortar preparation
    • C04B40/0039Premixtures of ingredients

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Ceramic Engineering (AREA)
  • Materials Engineering (AREA)
  • Structural Engineering (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of Bacillus alcaligenes to reinforce the purposes in calcareous sand technique as strengthening agent in mineralising.The present invention is found surprisingly that by research, Bacillus alcaligenes is added to mineralising as strengthening agent to reinforce in the grouting process of calcareous sand, on the one hand, compared with existing urase bacteria microorganism cement generates ammonia in hydrolysis urea process, citrate can be hydrolyzed to carbonate, carbonate therein and calcium binding generate calcium carbonate, and do not generate ammonia in mineralising consolidation process, effect on environment is smaller, more environmentally protective;On the other hand, compared with common portland cement, have bigger infiltration coefficient, can the more preferable draining and infiltration that must guarantee ground while reinforcing side slope and ground, to widen the application range of Bacillus alcaligenes in the market significantly.

Description

Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising
Technical field
The present invention relates to the new application of Bacillus alcaligenes, in particular to Bacillus alcaligenes reinforces conduct in calcareous sand technique in mineralising The purposes of strengthening agent
Background technique
Portland cement is gel rubber material the most commonly used in architectural engineering, in the historical progress and development from now on of the mankind In all occupy important position.But the production process of portland cement needs a large amount of heat to convert ripe stone for quick lime Ash, under existing productivity level, the heat of this part is generated by the burning of fossil fuel.So, silicic acid Need to consume a large amount of fossil fuel in the production process of salt cement, and during combustion of fossil fuels and firing quick lime A large amount of carbon dioxide (CO will be discharged2), CO in atmosphere2The promotion of concentration will cause " greenhouse effects ".In addition, silicate cement Various pernicious gases can be also generated in mud production process, and ecological environment is adversely affected.
Microorganism cement belongs to biogel material, and cardinal principle is the hydrolysis using microorganism, generates CO3 2- Or NH4 +Plasma, with loose material Ca as contained in sand2+Etc. sediment is formed, loose flocculation is got up, Play the role of cured.The gel rubber material traditional compared to portland cement etc., its production of biogel material and mechanism are equal It is more environmentally protective.In addition, the mechanism of biogel material can also be carried out carefully by controlling the growing environment of microorganism The regulation of cause, to realize the automation building course of " intelligence " and " controllable ".Microorganism cement is in side slope and foundation stabilization side Mask has wide practical use, and belongs to the research hotspot of civil engineering and bioengineering field in recent years.
But mainly using urase bacterium (Bacillus pasteurii) in common microorganism cement, the urase bacterium is micro- Biology generates urase, under the action of urase, urea can fast hydrolyzing generate ammonia and carbonate, carbonate can and solution in Calcium ion effect, generates calcium carbonate, forms gel, and loose sand grains is solidified.But there are also significant component of ammonia due to As reaction carries out solution ph raising, it can not dissolve and be directly discharged in air, have a negative impact to environment.Therefore, Ammonia emission of the existing microorganism cement material in mineralising consolidation is solved, really realizes the microorganism cement tool of green non-pollution There is highly important practical significance.
Summary of the invention
It is reinforced in calcareous sand technique to solve the above problems, the purpose of the present invention is to provide a kind of Bacillus alcaligenes in mineralising Purposes as strengthening agent.
Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising.
Wherein, the bacterial concentration OD of the Bacillus alcaligenes600=1.3-1.6, number GIM1.754, Latin literary fame are Alcaligenessp, other numbers are ACCC 01730, are bought in Guangdong Province's Organism Depositary.
Wherein, it is grouting process that the mineralising, which reinforces calcareous sand technique,.
Preferably, the grouting process, includes the following steps:
1) citrate is added in Bacillus alcaligenes solution, forms mixing liquid;
2) above-mentioned mixed solution peristaltic pump is injected in calcareous sand column, stands 1-3 hours;
3) again with peristaltic pump to the calcium salt soln of above-mentioned calcareous sand column injection 100mL, 5-7 hours are stood, by calcareous sand column In surplus solution discharge;
4) step 2) -3 is repeated), until it can not reinject mixing liquid into above-mentioned calcareous sand column;
5) the calcareous sand column good to above-mentioned reinforcing carries out drying maintenance demoulding.
Wherein, in step 1), the molarity of the citrate is 0.1-1mol/l.
Wherein, in step 1), the volume ratio of Bacillus alcaligenes and citrate is 0.5-1.5:1 in the mixed solution.
Wherein, in step 2), the dosage of the mixed solution is 50-150mL.
Wherein, in step 2), the size of the calcareous sand column is Ф 5cm × 10cm.
Wherein, in step 3), the calcium salt soln is selected from CaCl2One or more of solution, calcium nitrate, calcium lactate.
Wherein, in step 3), the molarity of the calcium salt soln is 0.1-1mol/l, volume 50-150mL.
Compared with prior art, the present invention having the following beneficial effects:
The present invention is found surprisingly that by research, is added to the filling that mineralising reinforces calcareous sand for Bacillus alcaligenes as strengthening agent In sizing process, on the one hand, can be by lemon compared with existing urase bacteria microorganism cement generates ammonia in hydrolysis urea process Lemon hydrochlorate is hydrolyzed to carbonate, and carbonate therein and calcium binding generate calcium carbonate, and do not produce in mineralising consolidation process Ammonifying gas, effect on environment is smaller, more environmentally protective;On the other hand, compared with common portland cement, have bigger Infiltration coefficient, can the more preferable draining and infiltration that must guarantee ground while reinforcing side slope and ground, to widen significantly The application range of Bacillus alcaligenes in the market.
Detailed description of the invention
Fig. 1 is the calcareous sand column schematic diagram of Φ 5cm × 10cm used by embodiment 1-3 and comparative example 1-4 reinforcement experiment;
Fig. 2 is the result figure for obtain after XRD analysis to the loose calcareous sand column of embodiment 1;
Fig. 3-7 is the scanning electron microscope (SEM) photograph of the calcareous sand column after the consolidation by grouting under the different amplification factor of embodiment 1.
Specific embodiment
Below in conjunction with attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that institute The embodiment of description is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the implementation in the present invention Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to The scope of protection of the invention.
In the embodiment of the present invention, raw material used in the present invention is as follows:
The bacterial concentration OD of Bacillus alcaligenes600=1.3-1.6, number are GIM 1.754, and Latin literary fame is Alcaligenessp, other numbers are ACCC 01730, are bought in Guangdong Province's Organism Depositary;
Citrate, calcium salt soln can be commercially available by commercially available.
The test method of each performance indicator:
Uniaxial compressive strength: after the completion of consolidation by grouting, Example 1-3 and comparative example 1-4 dry the sand column conserved, Uniaxial compressive test is carried out, its uniaxial compressive strength is tested;
Infiltration coefficient: the calcareous sand column after Example 1-3 and comparative example 1-4 reinforcing carries out permeability test, tests its infiltration Saturating coefficient;
Whether the test method of ammonia: GB/T 14679-1993 air quality-ammonia measurement-sodium hypochlorite-bigcatkin willow is generated Sour spectrophotometry.
Reinforce the mineralogical composition in the sand column of front and back: to the calcareous sand column before reinforcing and the calcareous sand after the reinforcing of embodiment 1 Column carries out XRD detection, and the mineralogical composition in the sand column of front and back is reinforced in detection;
It reinforces front and back calcareous sand column and surrounds and watches structure: to calcareous sand column before reinforcing and calcareous after the reinforcing of embodiment 1 The mode that scanning electron microscope is respectively adopted in sand column is scanned calcareous sand column, and calcareous sand column surrounds and watches structure before and after observation is reinforced.
Mineralising containing Bacillus alcaligenes reinforces calcareous sand grouting process:
Embodiment 1
1) citrate that molarity is 0.8mol/l is added to strain concentration OD600=1.5 Bacillus alcaligenes In solution, mixing liquid is formed, wherein the volume ratio of Bacillus alcaligenes and citrate is 1:1 in the mixed solution;
2) the above-mentioned mixed solution of 100mL is injected in the calcareous sand column of Ф 5cm × 10cm with peristaltic pump, stands 2 hours;
3) CaCl for being again 0.8mol/l with the molarity of from peristaltic pump to above-mentioned calcareous sand column injection 100mL2It is molten Liquid stands 6 hours, and the surplus solution in calcareous sand column is discharged;
4) step 2) -3 is repeated), until it can not reinject mixing liquid into above-mentioned calcareous sand column;
5) the calcareous sand column good to above-mentioned reinforcing carries out drying maintenance demoulding.Calcareous sand column after resulting reinforcing Consolidation effect
The calcareous sand column schematic diagram of Φ 5cm × 10cm used by the reinforcement experiment of calcareous sand column after resulting reinforcing is such as Shown in Fig. 1;The result obtained after XRD analysis is carried out to the calcareous sand column of the calcareous sand column after resulting reinforcing as shown in Fig. 2, figure In be shown in consolidation by grouting after, have in calcareous sand calcium carbonate (85-0849) generation, and the ingredient of calcareous sand be Aragonite (99-0013);Calcareous sand column of the calcareous sand column after the consolidation by grouting under different amplification factors after resulting reinforcing is swept Retouch electron microscope as shown in fig. 3 to 7;The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Embodiment 2
The volume ratio of Bacillus alcaligenes and citrate is 0.5:1 in the step 1) mixed solution, other are the same as embodiment 1. The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Embodiment 3
The volume ratio of Bacillus alcaligenes and citrate is 1.5:1 in the step 1) mixed solution, other are the same as embodiment 1. The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Comparative example 1
The volume ratio or molar ratio of Bacillus alcaligenes and citrate are 0.1:1 in the step 1) mixed solution, other are same Embodiment 1.The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Comparative example 2
The volume ratio or molar ratio of Bacillus alcaligenes and citrate are 2:1 in the step 1) mixed solution, other are the same as real Apply example 1.The consolidation effect of calcareous sand column after resulting reinforcing is as shown in table 1.
Comparative example 3
Bacillus alcaligenes is replaced using urase bacterium, while adding the urea of 0.1mol/l, other are the same as embodiment 1.It is resulting to add Gu the consolidation effect of calcareous sand column after is as shown in table 1.
Comparative example 4
Bacillus alcaligenes is replaced using Bacillus pasteurii, while adding the urea of 0.1mol/l, other are the same as embodiment 1.Institute The consolidation effect of calcareous sand column after the reinforcing obtained is as shown in table 1.
Table 1
The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all at this Under the inventive concept of invention, using equivalent structure transformation made by description of the invention and accompanying drawing content, or directly/use indirectly It is included in other related technical areas in scope of patent protection of the invention.

Claims (10)

1. Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising.
2. Bacillus alcaligenes according to claim 1 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special Sign is, the OD of the Bacillus alcaligenes600=1.3-1.6, number are GIM 1.754, Latin literary fame Alcaligenessp, It is ACCC 01730 that he, which numbers, is bought in Guangdong Province's Organism Depositary.
3. Bacillus alcaligenes according to claim 1 or 2 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, It is characterized in that, it is grouting process that the mineralising, which reinforces calcareous sand technique,.
4. Bacillus alcaligenes according to claim 3 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special Sign is that the grouting process includes the following steps:
1) citrate is added in Bacillus alcaligenes solution, forms mixing liquid;
2) above-mentioned mixed solution peristaltic pump is injected in calcareous sand column, stands 1-3 hours;
3) calcium salt soln is injected to above-mentioned calcareous sand column with peristaltic pump again, 5-7 hours is stood, by the surplus solution in calcareous sand column Discharge;
4) step 2-3 is repeated), until it can not reinject mixing liquid into above-mentioned calcareous sand column;
5) the calcareous sand column good to above-mentioned reinforcing carries out drying maintenance demoulding.
5. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special Sign is, in step 1), the molarity of the citrate is 0.1-1mol/l.
6. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special Sign is, in step 1), the volume ratio of Bacillus alcaligenes and citrate is 0.5-1.5:1 in the mixed solution.
7. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special Sign is, in step 2, the dosage of the mixed solution is 50-150mL.
8. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special Sign is, in step 2, the size of the calcareous sand column is Ф 5 cm × 10 cm.
9. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special Sign is, in step 3), the calcium salt soln is selected from CaCl2One or more of solution, calcium nitrate, calcium lactate.
10. Bacillus alcaligenes according to claim 4 reinforces the purposes in calcareous sand technique as strengthening agent in mineralising, special Sign is, in step 3), the molarity of the calcium salt soln is 0.1-1mol/l, volume 50-150mL.
CN201910638411.6A 2019-07-16 2019-07-16 Bacillus alcaligenes reinforces the purposes in calcareous sand technique as strengthening agent in mineralising Pending CN110330251A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110713959A (en) * 2019-11-28 2020-01-21 上海海事大学 Microbial agent for heavy metal pollution remediation and application thereof
CN111056782A (en) * 2019-12-31 2020-04-24 中交路桥华南工程有限公司 Microbial self-repairing method for tunnel lining concrete cracks
CN113123353A (en) * 2021-04-16 2021-07-16 合肥工业大学 Method for solidifying redbed slope surface soil by microbial preparation
CN113666407A (en) * 2021-08-20 2021-11-19 中山大学 Comprehensive utilization process for extracting calcium ions in south sea calcareous sand and treating algae sewage and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101644047A (en) * 2009-07-23 2010-02-10 东南大学 Method of adhering loose sands employing microbial mineralization
CN106856700A (en) * 2016-12-29 2017-06-20 河海大学 Desert planting structure and in desert planting plants method
WO2018013830A1 (en) * 2016-07-14 2018-01-18 Dong-Kyun Seo Antimicrobial geopolymer compositions

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101644047A (en) * 2009-07-23 2010-02-10 东南大学 Method of adhering loose sands employing microbial mineralization
WO2018013830A1 (en) * 2016-07-14 2018-01-18 Dong-Kyun Seo Antimicrobial geopolymer compositions
CN106856700A (en) * 2016-12-29 2017-06-20 河海大学 Desert planting structure and in desert planting plants method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110713959A (en) * 2019-11-28 2020-01-21 上海海事大学 Microbial agent for heavy metal pollution remediation and application thereof
CN111056782A (en) * 2019-12-31 2020-04-24 中交路桥华南工程有限公司 Microbial self-repairing method for tunnel lining concrete cracks
CN111056782B (en) * 2019-12-31 2021-07-30 中交路桥华南工程有限公司 Microbial self-repairing method for tunnel lining concrete cracks
CN113123353A (en) * 2021-04-16 2021-07-16 合肥工业大学 Method for solidifying redbed slope surface soil by microbial preparation
CN113666407A (en) * 2021-08-20 2021-11-19 中山大学 Comprehensive utilization process for extracting calcium ions in south sea calcareous sand and treating algae sewage and application thereof

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Application publication date: 20191015