CN115478863A - Granite stratum reinforcing method - Google Patents
Granite stratum reinforcing method Download PDFInfo
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- CN115478863A CN115478863A CN202211019291.XA CN202211019291A CN115478863A CN 115478863 A CN115478863 A CN 115478863A CN 202211019291 A CN202211019291 A CN 202211019291A CN 115478863 A CN115478863 A CN 115478863A
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- 239000010438 granite Substances 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 32
- 230000003014 reinforcing effect Effects 0.000 title claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 230000002787 reinforcement Effects 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 52
- 239000007788 liquid Substances 0.000 claims description 45
- 235000015097 nutrients Nutrition 0.000 claims description 42
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 20
- 239000001110 calcium chloride Substances 0.000 claims description 19
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 19
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 12
- 239000004202 carbamide Substances 0.000 claims description 12
- 239000011440 grout Substances 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 11
- 238000009630 liquid culture Methods 0.000 claims description 11
- 239000002245 particle Substances 0.000 claims description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- 241000193395 Sporosarcina pasteurii Species 0.000 claims description 10
- 238000002347 injection Methods 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 238000007596 consolidation process Methods 0.000 claims description 9
- 241000606860 Pasteurella Species 0.000 claims description 8
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims description 7
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 7
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 7
- 230000003321 amplification Effects 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 239000002609 medium Substances 0.000 claims description 7
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 claims description 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 7
- 241001668579 Pasteuria Species 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 2
- 241000606856 Pasteurella multocida Species 0.000 claims 1
- 229940051027 pasteurella multocida Drugs 0.000 claims 1
- 238000005728 strengthening Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 12
- 230000008569 process Effects 0.000 abstract description 11
- 230000035699 permeability Effects 0.000 abstract description 7
- 239000002689 soil Substances 0.000 abstract description 6
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 22
- 229910000019 calcium carbonate Inorganic materials 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 9
- 239000011259 mixed solution Substances 0.000 description 9
- 230000010412 perfusion Effects 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 150000003839 salts Chemical class 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 230000008595 infiltration Effects 0.000 description 4
- 238000001764 infiltration Methods 0.000 description 4
- 241000193469 Clostridium pasteurianum Species 0.000 description 3
- 108010046334 Urease Proteins 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
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- 239000002028 Biomass Substances 0.000 description 2
- 229910021532 Calcite Inorganic materials 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- 238000002441 X-ray diffraction Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
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- 239000002002 slurry Substances 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 241000179039 Paenibacillus Species 0.000 description 1
- 229910052656 albite Inorganic materials 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
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- 239000010453 quartz Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
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- 238000007873 sieving Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
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- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21D—SHAFTS; TUNNELS; GALLERIES; LARGE UNDERGROUND CHAMBERS
- E21D9/00—Tunnels or galleries, with or without linings; Methods or apparatus for making thereof; Layout of tunnels or galleries
- E21D9/001—Improving soil or rock, e.g. by freezing; Injections
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/91—Use of waste materials as fillers for mortars or concrete
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Abstract
The invention provides a granite stratum reinforcing method, which belongs to the technical field of civil engineering. The invention can finish soil body reinforcement and crack filling within 3-4 days, does not cause secondary pollution to the environment in the process, has lower cost of used reagents and better effect, obviously enhances the integral strength after the granite stratum is reinforced, reduces water seepage channels in the granite system, reduces the permeability and has better water stopping property.
Description
Technical Field
The invention relates to the technical field of civil engineering, in particular to a granite stratum reinforcing method.
Background
With the vigorous development of economic construction in China, the number of constructed tunnels is continuously increased, different geological conditions bring challenges to tunnel construction, and the scattered fully-weathered granite is a common stratum in tunnel engineering and is concerned about due to the variability of engineering properties, lower strength and stability and the regionality of engineering geological factors.
Granite stratums are widely distributed in coastal areas of southeast of Fujian, hot and rainy regional climatic environments enable the weathering effect of rock masses to be strong, the strength and the stability of the rock masses are greatly reduced, and water seepage and mud outburst disasters frequently occur when a tunnel is excavated in a water-rich strong-weathering stratum. Aiming at the characteristic that the granite stratum is softened and disintegrated when meeting water, advance curtain pre-grouting is often adopted to reinforce the granite stratum in the tunnel construction process. However, the final setting time of the cement slurry for grouting is too long, and piping is easy to occur under the driving action of a certain hydraulic pressure difference, so that the slurry is lost and runs, thereby not only wasting the cement material, but also failing to achieve good grouting water stop and strength effects.
Disclosure of Invention
The invention aims to provide a granite stratum reinforcing method to solve the problem of poor grouting reinforcing effect of a granite stratum in the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a granite stratum reinforcing method, which comprises the following steps:
s1, pouring completely weathered granite into a structural hollow drum, sealing a cavity, and reserving a grout inlet and a grout outlet;
s2, injecting a bacillus pasteurii bacterial liquid, and standing;
s3, injecting a stationary liquid, wherein the stationary liquid is a calcium chloride aqueous solution;
s4, injecting a nutrient solution, wherein the concentration ratio of urea to calcium chloride in the nutrient solution is 1;
and S5, repeating the steps S2 to S4 for 1 to 3 times.
Preferably, the mass volume ratio of the completely weathered granite on the seabed, the pasteurella bacteria liquid, the fixing liquid and the nutrient solution is 350-450g.
Preferably, the particle size of the seabed completely weathered granite is less than 10 mm.
Preferably, the concentration of the calcium chloride aqueous solution is 0.02 to 0.08mol/L.
Preferably, the total concentration of the nutrient solution is 0.5-1.5 mol/L.
Preferably, the injection speed of the pasteurella bacteria liquid, the calcium chloride aqueous solution and the nutrient solution is 2-5 ml/min.
Preferably, the bacillus pasteurianus bacterial liquid is obtained through the following steps:
inoculating the pasteur bacillus seed solution into a liquid culture medium, and carrying out expanded culture for 20-30 h to obtain the pasteur bacillus liquid.
Preferably, the liquid culture medium takes water as a solvent and comprises the following components in concentration: 10-30 g/L of yeast extract, 5-15 g/L of ammonium sulfate and 5-15 mu mol/L of nickel chloride.
Preferably, the pH of the liquid medium is 8.0 to 10.0.
Preferably, the volume ratio of the bacillus pasteurianus seed solution to the liquid culture medium is 1;
the temperature of the amplification culture is 25-35 ℃, and the rotation speed of the amplification culture is 100-200 rpm.
The invention has the technical effects and advantages that:
the Paenibacillus pasteurianus cultured by the invention has higher urease activity, can quickly catalyze urea to hydrolyze to generate carbonate ions, simultaneously utilizes the electrostatic action among bacterial cells to attract calcium ions in a solution environment to agglomerate on the surfaces of the cells, accelerates the formation of calcium carbonate, gels soil particles filled in hollow drums to form integral strength, can finish soil body reinforcement and crack filling within 3-4 days, cannot cause secondary pollution to the environment in the process, has lower cost of used reagents and better effect, obviously enhances the integral strength after granite stratum reinforcement, reduces water seepage channels in a granite system, reduces permeability and has better water stopping performance.
Drawings
FIG. 1 is a particle size grading curve diagram of completely weathered seabed granite;
FIG. 2 is a diagram showing the changes in the biomass of a bacterial liquid and the activity of urease during the culture of the bacterial liquid;
FIG. 3 is a graph showing the relationship between the number of times of grouting and the amount of calcium carbonate generated in a sample under different concentrations of a nutrient solution;
FIG. 4 is a graph showing the relationship between the number of times of grouting and the dry density of a sample under different concentrations of a nutrient solution;
FIG. 5 is a relation between different grouting times and unconfined compressive strength of a sample under different nutrient solution concentrations;
FIG. 6 is a comparative analysis of XRD results before and after sample consolidation;
FIG. 7 is an SEM result analysis of the sample after consolidation.
Detailed Description
The invention provides a granite stratum reinforcing method, which comprises the following steps:
s1, pouring completely weathered granite into a structural hollow drum to seal a cavity, and reserving a grout inlet and a grout outlet;
s2, injecting a bacillus pasteurii bacterial liquid, and standing;
s3, injecting a fixing solution, wherein the fixing solution is a calcium chloride aqueous solution;
s4, injecting a nutrient solution, wherein the molar concentration ratio of urea to calcium chloride in the nutrient solution is 1;
and S5, repeating the steps S2 to S4 for 1 to 3 times.
In the invention, the totally weathered subsea granite is preferably granular, and the granular size of the totally weathered subsea granite is preferably 10mm or less, and more preferably 5mm or less; the mass-volume ratio of the totally weathered granite on the seabed, the pasteurella bacteria liquid, the fixing liquid and the nutrient solution is preferably 350-450g, 400-30ml, and more preferably 380-420g, and the mass-volume ratio of the fixing liquid to the nutrient solution is 24-26ml; the concentration of the calcium chloride aqueous solution is preferably 0.02-0.08 mol/L, and more preferably 0.04-0.05 mol/L; the molar concentration ratio of urea to calcium chloride in the nutrient solution is 1.8-1.2, preferably 1.9-1.1, the concentration of the nutrient solution is preferably 0.5-1.5 mol/L, more preferably 0.8-1.2 mol/L, and the concentration of the nutrient solution refers to the molar concentration of total solutes contained in the nutrient solution; in the present invention, the injection rate of the pasteurella bacteria liquid, the calcium chloride aqueous solution and the nutrient solution is preferably 2 to 5ml/min, and more preferably 3 to 4ml/min.
In the present invention, the pasteuria bacillus liquid is preferably obtained by the following steps: inoculating the pasteuria bacillus seed solution into a liquid culture medium, and performing amplification culture for 20-30 h to obtain a pasteuria bacillus bacterial solution, wherein the volume ratio of the pasteuria bacillus seed solution to the liquid culture medium is preferably 1-60, and more preferably 1-45-55, and the liquid culture medium preferably comprises the following components: 10-30 g/L of yeast extract, 5-15 g/L of ammonium sulfate and 5-15 mu mol/L of nickel chloride, wherein the concentration of the yeast extract in the liquid culture medium is preferably 10-30 g/L, more preferably 15-25 g/L, the concentration of the ammonium sulfate in the liquid culture medium is preferably 5-15 g/L, more preferably 8-12 g/L, the concentration of the nickel chloride in the liquid culture medium is preferably 5-15 mu mol/L, more preferably 8-12 mu mol/L, and the pH of the liquid culture medium is preferably 8.0-10.0, more preferably 8.5-9.5; in the present invention, the temperature of the amplification culture is preferably 25 to 35 ℃, more preferably 28 to 32 ℃, and the rotation speed of the amplification culture is preferably 100 to 200rpm, more preferably 140 to 170rpm.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
The strain sources used by the invention are as follows: bacillus Pasteurii (Sporosarcina Pasteurii) purchased from American type culture collection, accession number ATCC11859.
Example 1
Culturing single colony of Bacillus pasteurianus (Sporosarcina Pasteurii) in fermentation medium containing 20.0g, 10g ammonium sulfate, 10 μmol nickel chloride, and 1L distilled water at 25 deg.C for 24 hr to obtain bacterial liquid, and monitoring bacterial liquid biomass (by OD) every two hr during culture 600 Values are shown) and urease activity (urea hydrolysis rate is shown), the results are shown in figure 1. Carry out the water treatment that closes to the structure hollowing, pour into 380g seabed completely weathered granite, seal whole cavity, reserve the business turn over thick liquid mouth, test the performance of seabed completely weathered granite before pouring into, the result shows: the nonuniform coefficient Cu of the sample was 27.5, the curvature coefficient Cc was 1.54, and the sample was classified into well-graded soil, and the grain size grading curve of the fully well-graded soil is shown in FIG. 2. Injecting 100ml of bacterial liquid through a reserved injection port at the speed of 3ml/min, and ensuring that the microbial liquid fully wraps the seabed completely weathered granite particles; standing after the injection is completed, and then making them pass through the reserved injection hole to make them identical25ml of calcium chloride solution with the concentration of 0.05mol/L is injected at a speed; then 500ml of urea and calcium chloride mixed solution (nutrient solution) with equal molar concentration is injected, the total concentration of the mixed solution is 0.75mol/L, the processes of bacterium solution perfusion, calcium chloride solution perfusion and urea and calcium chloride mixed solution perfusion are repeated, and the circulation is performed for 4 times totally.
Example 2
A single colony of Bacillus Pasteurii (Sporosarcina Pasteurii) was cultured at 30 ℃ for 18 hours in a fermentation medium containing 15.0g of ammonium sulfate, 11. Mu. Mol of nickel chloride and 1L of distilled water at pH 9.0 to obtain a bacterial liquid. Carrying out water closing treatment on the structural hollowing, pouring 350g of seabed completely weathered granite, sealing the whole cavity, and reserving a grout inlet and a grout outlet; injecting 120ml of bacterial liquid through a reserved grouting port at the speed of 3ml/min, and ensuring that the microbial liquid fully wraps the seabed completely weathered granite particles; after the injection is finished, standing, and injecting 20ml and 0.04mol/L calcium chloride solution at the same speed through a reserved injection port; then 520ml of urea and calcium chloride mixed solution (nutrient solution) with equal molar concentration is injected, the total concentration of the mixed solution is 0.5mol/L, the processes of bacterium solution perfusion, calcium chloride solution perfusion and urea and calcium chloride mixed solution perfusion are repeated, and the circulation is performed for 3 times totally.
Example 3
A single colony of Bacillus Pasteurii (Sporosarcina Pasteurii) was cultured at 31 ℃ for 18 hours in a fermentation medium containing 18.0g of ammonium sulfate, 9g of 14. Mu. Mol of nickel chloride and 1L of distilled water at pH 9.0 to obtain a bacterial liquid. Carrying out water closing treatment on the structural hollowing, pouring 400g of seabed completely weathered granite, sealing the whole cavity, and reserving a grout inlet and a grout outlet; injecting 100ml of bacterial liquid through a reserved grouting port at the speed of 3ml/min, and ensuring that the microbial liquid fully wraps the seabed completely weathered granite particles; standing after the injection is finished, and then injecting 30ml of 0.04mol/L calcium chloride solution through a reserved injection port at the same speed; 550ml of urea and calcium chloride mixed solution (nutrient solution) with equal molar concentration is injected, the total concentration of the mixed solution is 1.5mol/L, the processes of bacterium solution perfusion, calcium chloride solution perfusion, urea and calcium chloride mixed solution perfusion are repeated, and the circulation is performed for 2 times totally.
Experimental example 1 analysis of physical and mechanical Properties
Permeability analysis was performed on the consolidated 3d granite formation of example 1 by the following analysis method:
a permeability analysis (model: TST-55 type soil permeameter) test adopts a variable water head test method, a cutting ring provided with a sample is arranged in the permeability instrument, and is screwed by a nut until water and air do not leak. Connecting the water inlet of the infiltration container with a water head changing pipe, filling the water inlet pipe with pure water in a water supply bottle, infiltrating the infiltration container, opening an exhaust valve, exhausting air at the bottom of the infiltration container until no bubbles exist in overflowing water, closing the exhaust valve, flatly placing the infiltration container, and closing a water pipe clamp. Injecting pure water into the water changing head pipe to enable the water to rise to a preset height, cutting off a water source after the water level is stable, opening the water inlet pipe clamp to enable the water to pass through the sample, starting to measure and record the height and the starting time of the initial water head in the water changing head pipe when the water overflows from the water outlet, measuring and recording the change of the water head and the time according to a preset time interval, and measuring and recording the water temperature of the water outlet:
wherein a is the cross-sectional area of the variable water head pipe and the unit is cm 2 (ii) a A is the cross-sectional area of the sample in cm 2 (ii) a 2.3 transform factors of ln and log; l is the seepage diameter, namely the height of the sample, and the unit is cm; t is t 1 、t 2 Respectively the starting time and the ending time of the reading head, and the unit is s; h 1 、H 2 Respectively, initial and final water.
The results show that: the reinforcing process of the invention gradually reduces the porosity among the original loose granite particles, the porosity is sharply reduced, compared with the permeability coefficient (2.02 multiplied by 10 < -2 > cm/s) of the original seabed fully-differentiated granite, the reinforced seabed fully-differentiated granite stratum sample (4.07 multiplied by 10 < -4 > cm/s) is obviously reduced by two orders of magnitude, which shows that the invention can play a significant water stopping effect.
And (3) testing the uniaxial compressive strength of the reinforced seabed fully-differentiated granite stratum sample (equipment model ETM 205D), and polishing two ends of the sample to be flat so that the size of the sample is a standard uniaxial sample with the diameter of 50mm and the height of 100 mm. The test process adopts a displacement control method, the loading rate is 1mm/min, the preload is set to be 100N, the process from the loading to the sample destruction is recorded, and a stress-strain curve is obtained. The relationship between the concentration of different nutrient solutions and the grouting times, the content of calcium carbonate, the dry density of the sample and the unconfined compressive strength of the sample is recorded at the same time, and the results are shown in FIGS. 3-5.
From the results, it can be seen that: under the condition of nutrient salt with different concentrations, after 1-4 batches of grouting, the trend of the calcium carbonate content change curve of a test object is similar to that of the dry density change curve, the calcium carbonate content and the dry density of a sample are increased along with the increase of the grouting times, the corresponding unconfined compressive strength is also increased, but the trend of the unconfined compressive strength change curve is different from that of the calcium carbonate content change curve, and the unconfined compressive strength is improved by 5-8 times compared with that of grouting for 1 time and 2 times for 3 times and 4 times under the conditions of 0.5mol/L and 0.75mol/L of nutrient salt concentration. The concentration of the nutrient salt is 0.75mol/L, and the strength value reaches the maximum value of 2.43Mpa after 4 times of grouting. And then, the strength value is gradually reduced after 3 times and 4 times of grouting along with the increase of the concentration of the nutrient salt, compared with 1 time and 2 times of grouting, the strength is increased in a smaller and smaller range, and the analysis result shows that when the concentration of the nutrient solution is higher than the optimal concentration, the existence of the pasteurella is inhibited due to the overhigh concentration of calcium ions, so that the generation amount of calcium carbonate is reduced, and the strength is reduced. When the concentration of the nutrient solution is higher than the optimal concentration, the transmission of the bacterial solution is not facilitated in the grouting process because the seabed fully-differentiated granite contains more clay particles. Secondly, along with the increase of the grouting times and the concentration of nutrient salts, calcium carbonate rapidly generated at the grout inlet end of the sample also blocks the transmission of bacteria liquid and nutrient solution, so that the blockage of the grout inlet end and the uneven transmission of the bacteria liquid and the nutrient solution occur, and the integral strength of the reinforced sample is influenced. When the concentration of the nutrient solution is lower than or equal to the optimal concentration, the speed of generating calcium carbonate is slow, and the transportation channel of the bacterial liquid and the nutrient solution is not enough blocked in the grouting period, so that the bacterial liquid and the nutrient solution are uniformly distributed in the sample system, and the overall strength of the sample is improved.
Example 2 microscopic Property testing
The submarine granite samples before and after consolidation were subjected to mineral diffraction (XRD) analysis (the experimental instrument was a field emission scanning electron microscope (Nova NanoSEM 450), and the samples were divided into small pieces (10 mm) 3 Within) on the conducting resin, using an aurilave to blow loose powder off, then carrying out gold spraying treatment, putting the sample into an instrument for vacuumizing, observing under the voltage of 15-20 kV, and then shooting photos with different magnification according to requirements. As shown in FIG. 6, FIG. 6 shows the fully weathered granite on the seabed as it is (a) and the reinforced granite sample on the seabed as the invention.
As can be seen from fig. 6, the main mineral components of the undisturbed completely weathered granite are quartz, albite and calcite, while the mineral components after reinforcement are unchanged, and the analysis by combining the added nutrient salt components can obtain: calcium carbonate is mainly generated in the grouting and reinforcing process, and the cementing effect is achieved.
And (2) carrying out microscopic morphology testing (SEM) on the reinforced sample, adopting an X-ray diffraction analysis (X-ray diffraction) method, using an X-ray diffractometer to carry out the test, mashing the reinforced sample by using a mortar, sieving the mashed sample by using a 0.01mm fine sieve, uniformly paving the sample on a Cu target, putting the sample into the instrument, setting the scanning rate to be 6 degrees/min and the scanning angle to be 5-90 degrees to obtain an XRD original spectrogram, and analyzing by using software Jade 6.5. The analysis results are shown in FIG. 7.
As can be seen from fig. 7, except for a part of pores, the granite particles on the seabed are all wrapped and filled with cubic calcite and have a gelling effect.
According to the embodiment, the reinforcing method provided by the invention enables the original loose granite particles to have gradually reduced pores, sharply reduced porosity and obviously reduced permeability coefficient, and the method can achieve a remarkable water stopping effect. The calcium carbonate generating rate in the concentration range of the nutrient solution provided by the invention is slow, and the transportation channel of the bacterial liquid and the nutrient solution can not be blocked in the grouting period, so that the bacterial liquid and the nutrient solution are uniformly distributed in a sample system, and the integral strength of the sample is improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A granite stratum reinforcing method is characterized by comprising the following steps:
s1, pouring completely weathered granite into a structural hollow drum, sealing a cavity, and reserving a grout inlet and a grout outlet;
s2, injecting a bacillus pasteurii bacterial liquid, and standing;
s3, injecting a fixing solution, wherein the fixing solution is a calcium chloride aqueous solution;
s4, injecting a nutrient solution, wherein the molar concentration ratio of urea to calcium chloride in the nutrient solution is 1;
and S5, repeating the steps from S2 to S4 for 1 to 3 times.
2. The granite ground layer reinforcement method of claim 1, wherein the mass volume ratio of the seabed completely weathered granite, the Pasteurella bacteria liquid, the fixing liquid and the nutrient liquid is 350-450g.
3. The granite formation reinforcing method of claim 1 or 2, wherein the particle size of the seabed fully weathered granite is below 10 mm.
4. The granite formation consolidation method of claim 3, wherein the concentration of the calcium chloride aqueous solution is 0.02-0.08 mol/L.
5. The granite formation consolidation method of claim 4, wherein the total concentration of the nutrient solution is 0.5-1.5 mol/L.
6. The granite ground consolidation method of claim 1 or 2, wherein the injection speed of the pasteuria bacillus liquid, the calcium chloride aqueous solution and the nutrient solution is 2-5 ml/min.
7. The granite ground layer strengthening method of claim 6, wherein the Pasteurella multocida liquid is obtained through the following steps:
inoculating the pasteurella bacillus seed liquid into a liquid culture medium, and carrying out expanded culture for 20-30 h to obtain the pasteurella bacillus liquid.
8. The granite formation consolidation method of claim 7, wherein the liquid medium is water as a solvent and comprises the following components in the following concentrations: 10-30 g/L of yeast extract, 5-15 g/L of ammonium sulfate and 5-15 mu mol/L of nickel chloride.
9. The granite formation consolidation method of claim 8, wherein the pH of the liquid medium is 8.0-10.0.
10. The granite ground layer consolidation method of claim 9, wherein the volume ratio of the pasteuria bacillus seed solution to the liquid medium is 1;
the temperature of the amplification culture is 25-35 ℃, and the rotation speed of the amplification culture is 100-200 rpm.
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| CN118562681A (en) * | 2024-07-11 | 2024-08-30 | 中国南方电网有限责任公司超高压输电公司电力科研院 | Bacillus cereus GZ-11-1 and method for solidifying granite residual soil |
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