CN114456934B - Intelligent automatic culture device for tissue culture - Google Patents
Intelligent automatic culture device for tissue culture Download PDFInfo
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- CN114456934B CN114456934B CN202210197815.8A CN202210197815A CN114456934B CN 114456934 B CN114456934 B CN 114456934B CN 202210197815 A CN202210197815 A CN 202210197815A CN 114456934 B CN114456934 B CN 114456934B
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- 235000015097 nutrients Nutrition 0.000 claims abstract description 48
- 230000007246 mechanism Effects 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims description 50
- 238000003860 storage Methods 0.000 claims description 21
- 238000005086 pumping Methods 0.000 claims description 16
- 238000007789 sealing Methods 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 239000013589 supplement Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 8
- 230000010355 oscillation Effects 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 230000000149 penetrating effect Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses an intelligent automatic culture device for tissue culture, which comprises a device shell, wherein the device shell is provided with a culture tank, the bottom wall of the device shell is provided with two first sliding grooves, the first sliding grooves are in sliding connection with first sliding blocks, the two first sliding blocks are in sliding connection with a placing plate together, and an oscillating mechanism is arranged in the device shell; the vibration mechanism comprises a functional cavity arranged in the bottom wall of the device shell, the inner top wall of the functional cavity is fixedly connected with a miniature motor, an output shaft of the miniature motor is in interference fit with a first gear, a second conductive block is fixedly embedded in the lower surface of the first gear, and a plurality of third conductive blocks and fourth conductive blocks are fixedly embedded in the inner bottom wall of the functional cavity. The advantages are that: the invention can intelligently and automatically adjust the oscillating force, ensure the oscillating effect, automatically supplement nutrient solution, and avoid the pollution of substances such as external bacteria and the like to the culture body without opening the device during the supplement.
Description
Technical Field
The invention relates to the technical field of tissue culture, in particular to an intelligent automatic culture device for tissue culture.
Background
Tissue culture, also known as in vitro culture, is one of the important means in biological research, meaning the separation of desirable tissue from plant bodies. Organs or cells, protoplasts, etc., are cultured under controlled conditions by aseptic manipulation to obtain regenerated whole plants or to produce other products of economic value, which are commonly used in plant breeding, plant detoxification and rapid propagation, plant useful product production, plant germplasm resource preservation and exchange. In aspects of genetics, physiology, biochemistry, pathology research and the like, a culture device is generally required to be used for continuously vibrating a culture dish in the tissue culture process, so that cell bottoms are piled up to influence development and propagation.
In the prior art, the oscillating force of the existing culture device is always fixed, when tissue culture is carried out on different quantities, the oscillating force cannot be automatically adjusted, the excessive oscillating force can cause material in the culture dish to overflow, the too small oscillating force can cause uneven oscillation, and the use is inconvenient.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, vibration force cannot be automatically adjusted, bacteria are easy to enter due to the fact that nutrient solution is added into equipment is opened, and the like.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the intelligent automatic culture device for tissue culture comprises a device shell, wherein a culture groove is formed in the device shell, two first sliding grooves are formed in the bottom wall of the device shell, first sliding blocks are connected in a sliding mode in the first sliding grooves, a placing plate is connected in a sliding mode together with the two first sliding blocks, and a vibration mechanism is arranged in the device shell;
the vibration mechanism comprises a functional cavity arranged in the bottom wall of the device shell, the top wall of the functional cavity is fixedly connected with a miniature motor, an output shaft of the miniature motor is provided with a first gear in an interference fit mode, a second conductive block is fixedly embedded in the lower surface of the first gear, a plurality of third conductive blocks and fourth conductive blocks are fixedly embedded in the bottom wall of the functional cavity, two opposite inner side walls of the culture tank are fixedly connected with first electromagnets, the third conductive blocks and the fourth conductive blocks are respectively connected with the corresponding first electromagnets through wires, one of the first electromagnets is provided with a mounting groove, the inner wall of the mounting groove is fixedly provided with a resistor coil, and one of the first conductive blocks is fixedly connected with the lower surface of the first slider.
Further, be provided with the pump liquid mechanism that is used for nutrient solution supply in the device shell, pump liquid mechanism includes two pump liquid groove of seting up in the device shell, pump liquid groove and function chamber intercommunication, sealed sliding connection has the pump board in the pump liquid groove, the function intracavity bottom is connected with two dwang through the bearing rotation, dwang interference fit has the second gear, second gear and first gear engagement, the second gear upper surface is connected with the connecting rod through the round pin axle rotation, the one end that the second gear was kept away from to the connecting rod is connected with corresponding pump board lateral wall through the round pin axle rotation, two liquid storage cavities have been seted up to the device shell, the device shell runs through fixedly connected with two first pipe fittings, second pipe fitting, third pipe fitting, first pipe fitting, second pipe fitting one end run through and extend to corresponding in the pump liquid groove, the other end runs through and extends to corresponding in the liquid storage cavity, third pipe fitting one end runs through and extends to corresponding pump liquid groove, the other end runs through and extends to in the culture tank.
Further, be provided with the control mechanism who is used for controlling pump liquid mechanism in the device shell, control mechanism is including seting up the cavity in the device shell, the lateral wall fixedly connected with second electro-magnet of cavity one side, the lateral wall fixedly connected with third electro-magnet of opposite side, the second electro-magnet passes through the wire with first electro-magnet and is connected, the third sliding tray has all been seted up to roof and interior diapire in the cavity, sliding connection has the fifth conducting block in the third sliding tray, fixedly connected with a plurality of second springs between fifth conducting block and the third sliding tray inner wall, the cavity inside wall is fixedly inlayed and is equipped with the sixth conducting block, be provided with first solenoid valve in the second pipe fitting, be provided with the second solenoid valve in the third pipe fitting, fifth conducting block, sixth conducting block, first solenoid valve, second solenoid valve pass through the wire and connect.
Further, the control mechanism further comprises a force-sensitive resistor fixedly embedded in the upper surface of the placing plate, the force-sensitive resistor is electrically connected with the third electromagnet through a wire, two electric push rods are fixedly connected to the inner bottom wall of the culture tank, and a stop plate is fixedly connected to the output end of each electric push rod.
Further, two second sliding grooves are formed in the lower surface of the placement plate, second sliding blocks are connected in the second sliding grooves in a sliding mode, the second sliding blocks are fixedly connected with the corresponding first sliding blocks, and the cross sections of the second sliding grooves and the second sliding blocks are T-shaped.
Further, a plurality of anti-slip pads are fixedly embedded on the upper surface of the placement plate.
Further, check valves are arranged in the first pipe fitting, the second pipe fitting and the third pipe fitting.
Further, a rubber pad is fixedly connected to the side wall of the stop plate, which is close to one side of the corresponding first sliding block.
The invention has the following advantages:
1. during culture, the culture dish is placed on the placement plate, the placement plate slides downwards to different degrees according to the total mass of the culture dish, so that the contact position of the first conductive block and the resistance coil is changed, the resistance of the first electromagnet is changed, the magnetic force of the first electromagnet is changed, the two first electromagnets are alternately electrified by matching with the rotation of the micro motor, the placement plate drives the culture dish to vibrate, the vibration force can be automatically adjusted according to the weight of the culture dish, and excessive material in the culture dish caused by overlarge vibration force is effectively avoided, and vibration unevenness caused by overlarge vibration force is avoided;
2. in the culture process, after the electric push rod is electrified, the position of the first slide block is locked, meanwhile, the weight of the culture dish is sensed through the force-sensitive resistor, meanwhile, the magnetic force of the second electromagnet is determined by the position of the first slide block, the magnetic force of the first slide block is not changed due to the position locking, the magnetic force of the third electromagnet is determined by the force-sensitive resistor, the magnetic force is changed along with the weight change of the culture dish, the magnetic force of the third electromagnet is consistent with the magnetic force of the second electromagnet initially, when the nutrient solution is insufficient, the magnetic force of the third electromagnet is reduced, so that the fifth conductive block is contacted with the sixth conductive block, two electromagnetic valves are electrified, the reciprocating motion of the pump plate can automatically supplement the nutrient solution, and the nutrient solution can be automatically supplemented timely, so that the normal culture is ensured;
3. the pump plate is used for automatically supplementing the nutrient solution, and the nutrient solution does not need to be added by the opening device, so that the problem that when the opening device is used for supplementing the nutrient solution, external bacteria and viruses enter the device to influence the experimental result of tissue culture is effectively avoided;
4. when the nutrient solution is not needed to be supplemented, the first electromagnetic valve is opened, the second electromagnetic valve is closed, at the moment, the reciprocating motion of the pump plate can pump the nutrient solution in the liquid storage cavity into the liquid pumping groove through the first pipe fitting, and the nutrient solution is pumped back into the liquid storage cavity through the second pipe fitting, so that the nutrient solution in the liquid storage cavity keeps flowing continuously, and the mixing uniformity of the nutrient solution is ensured at all times.
Drawings
FIG. 1 is a schematic diagram of a structure of an intelligent automatic culture device for tissue culture;
FIG. 2 is an enlarged view of a portion of FIG. 1;
fig. 3 is an enlarged view at a in fig. 1;
FIG. 4 is an enlarged view at B in FIG. 1;
FIG. 5 is an enlarged view at C in FIG. 1;
FIG. 6 is a partial cross-sectional view taken at D-D in FIG. 1;
FIG. 7 is a partial cross-sectional view taken at E-E of FIG. 1;
FIG. 8 is a schematic diagram of the circuit connection of an intelligent automated culture device for tissue culture.
In the figure: 1 device housing, 2 culture tank, 3 first sliding groove, 4 first sliding block, 5 placing plate, 6 culture dish, 7 first spring, 8 mounting groove, 9 resistance coil, 10 first conductive block, 11 functional cavity, 12 micro motor, 13 first gear, 14 second conductive block, 15 third conductive block, 16 fourth conductive block, 17 first electromagnet, 18 second sliding groove, 19 second sliding block, 20 non-slip pad, 21 pump tank, 22 pump plate, 23 rotating rod, 24 second gear, 25 connecting rod, 26 liquid storage cavity, 27 first pipe, 28 second pipe, 29 first solenoid valve, 30 third pipe, 31 cavity, 32 third sliding groove, 33 fifth conductive block, 34 second spring, 35 sixth conductive block, 36 force sensitive resistor, 37 second electromagnet, 38 third electromagnet, 39 electric push rod, 40 stop plate, 41 second solenoid valve.
Detailed Description
Referring to fig. 1-8, an intelligent automatic culture device for tissue culture comprises a device housing 1, wherein the device housing 1 is connected with a device cover through a hinge, a culture tank 2 is arranged on the device housing 1, two first sliding grooves 3 are arranged on the bottom wall of the device housing 1, a first sliding block 4 is connected in a sliding manner in the first sliding grooves 3, two first sliding blocks 4 are connected with a placing plate 5 in a sliding manner together, the placing plate 5 is made of metal, and an oscillation mechanism is arranged in the device housing 1;
the oscillating mechanism comprises a functional cavity 11 arranged in the bottom wall of the device shell 1, the inner top wall of the functional cavity 11 is fixedly connected with a micro motor 12, an output shaft of the micro motor 12 is in interference fit with a first gear 13, a second conductive block 14 is fixedly embedded on the lower surface of the first gear 13, an insulating coating is coated between the first gear 13 and the second conductive block 14, so that the first gear 13 is prevented from being communicated with the second conductive block 14, a plurality of third conductive blocks 15 and fourth conductive blocks 16 are fixedly embedded on the inner bottom wall of the functional cavity 11, as shown in fig. 7, the third conductive blocks 15 and the fourth conductive blocks 16 are alternately arranged, so that in the process of driving the first gear 13 to rotate by the micro motor 12, the second conductive blocks 14 are continuously alternately contacted with the third conductive blocks 15 and the fourth conductive blocks 16 in the process of rotating by the first gear 13, the two opposite inner side walls of the culture tank 2 are fixedly connected with first electromagnets 17, as shown in fig. 1, the left inner side wall and the right inner side wall are fixedly connected with first electromagnets 17, a third conductive block 15 and a fourth conductive block 16 are respectively connected with corresponding first electromagnets 17 through wires, the third conductive block 15 is connected with the first electromagnets 17 on the left side, the fourth conductive block 16 is connected with the first electromagnets 17 on the right side, so that the second conductive block 14 is alternately electrified in the alternating contact process with the third conductive block 15 and the fourth conductive block 16, the placing plate 5 is continuously oscillated through magnetic force generated alternately to drive the culture dish 6 to oscillate, the inner side wall of one first sliding groove 3 is provided with a mounting groove 8, the inner wall of the mounting groove 8 is fixedly provided with a resistor coil 9, the lower surface of one first sliding block 4 is fixedly connected with the first conductive block 10, the circuit connection relationship is shown in fig. 8, the resistance coil 9 is connected with one pole of the power supply, the second conducting block 14 is connected with the first conducting block 10, the second electromagnet 37, the first electromagnet 17, the third conducting block 15 and the fourth conducting block 16 are connected and then connected with the other pole of the power supply, so that the contact position of the first conducting block 10 and the resistance coil 9 can be changed when the first conducting block 10 slides downwards, when the weight of the culture dish 6 is heavier, the sliding position of the first conducting block 10 is larger, the resistance value of the first electromagnet 17 is reduced, the magnetic force of the first electromagnet 17 is increased, the magnetic attraction of the first electromagnet 17 to the placing plate 5 is increased, and when the weight of the culture dish 6 is lighter, the magnetic attraction of the first electromagnet 17 to the placing plate 5 is reduced, and the oscillating force of the placing plate 5 can be automatically adjusted according to the weight of the culture dish 6, so that the substances in the culture dish 6 overflow due to overlarge oscillating force is effectively avoided, the oscillating force is uneven due to overlarge substances in the culture dish 6, and the device is more intelligent and automatic.
It should be noted that when the oscillation frequency needs to be changed, the rotation speed of the micro motor 12 can be changed, so as to change the frequency of the second conductive block 14 in alternate contact with the third conductive block 15 and the fourth conductive block 16, that is, the alternate frequency of the alternate energization of the first electromagnet 17 can be changed, that is, the reciprocating oscillation frequency of the placing plate 5 can be changed, so as to change the oscillation frequency.
The device housing 1 is internally provided with a liquid pumping mechanism for supplying nutrient solution, the liquid pumping mechanism comprises two liquid pumping tanks 21 which are arranged in the device housing 1, the liquid pumping tanks 21 are communicated with the functional cavities 11, pump plates 22 are connected in the liquid pumping tanks 21 in a sealing sliding manner, the inner bottom wall of the functional cavities 11 is rotationally connected with two rotating rods 23 through bearings, the rotating rods 23 are in interference fit with second gears 24, the second gears 24 are meshed with the first gears 13, the upper surfaces of the second gears 24 are rotationally connected with connecting rods 25 through pin shafts, one ends of the connecting rods 25, far away from the second gears 24, are rotationally connected with the corresponding side walls of the pump plates 22 through pin shafts, the device housing 1 is provided with two liquid storage cavities 26, the device housing 1 is fixedly connected with two first pipe fittings 27, second pipe fittings 28 and third pipe fittings 30 in a penetrating manner, one ends of the first pipe fittings 27 and the second pipe fittings 28 extend into the corresponding liquid pumping tanks 21 in a penetrating manner, the other end of the third pipe fitting 30 extends to the corresponding liquid storage cavity 26 in a penetrating way, one end of the third pipe fitting 30 extends to the corresponding liquid pumping groove 21 in a penetrating way, the other end extends to the culture groove 2 in a penetrating way, as shown in fig. 1, the length of the first pipe fitting 27 is longer than that of the second pipe fitting 28, so that the first pipe fitting 27 stretches into the bottom of the nutrient solution in the liquid storage cavity 26, the second pipe fitting 28 does not stretch into the nutrient solution, the first gear 13 rotates and simultaneously drives the second gear 24 meshed with the first gear to rotate, the second gear 24 drives the pump plate 22 to reciprocate through the connecting rod 25, the reciprocating motion of the pump plate 22 can pump the nutrient solution in the liquid storage cavity 26 into the liquid pumping groove 21 through the first pipe fitting 27 and then pump the nutrient solution back into the liquid storage cavity 26 through the second pipe fitting 28 under the control of the control mechanism, so that the nutrient solution in the liquid storage cavity 26 keeps flowing continuously, thereby ensuring uniform mixing of the nutrient solution at all times, and the nutrient solution can be replenished into the culture dish 6 through the third pipe fitting 30 when replenishing the nutrient solution.
The device shell 1 is internally provided with a control mechanism for controlling a liquid pumping mechanism, the control mechanism comprises a cavity 31 arranged in the device shell 1, the side wall of one side of the cavity 31 is fixedly connected with a second electromagnet 37, the side wall of the other side is fixedly connected with a third electromagnet 38, the second electromagnet 37 is connected with a first electromagnet 17 through a wire, the inner top wall and the inner bottom wall of the cavity 31 are both provided with a third sliding groove 32, a fifth conductive block 33 is connected in the third sliding groove 32 in a sliding way, a plurality of second springs 34 are fixedly connected between the fifth conductive block 33 and the inner wall of the third sliding groove 32, a sixth conductive block 35 is fixedly embedded in the inner wall of the cavity 31, a first electromagnetic valve 29 is arranged in a second pipe fitting 28, the first electromagnetic valve 29 is a normally open electromagnetic valve, the third pipe fitting 30 is internally provided with a second electromagnetic valve 41, the second electromagnetic valve 41 is a normally closed electromagnetic valve, the switch is opened in a power-on way, the fifth conductive block 33, the sixth conductive block 35, the first electromagnetic valve 29 and the second electromagnetic valve 41 are connected through wires, after the fifth conductive block 33 is contacted with the sixth conductive block 35, the first electromagnetic valve 29 and the second electromagnetic valve 41 can be electrified, in the culture process, the force-sensitive resistor 36 senses the gravity of the culture dish 6 in real time, when the nutrient solution in the culture dish 6 is reduced to a certain degree, the resistance value of the force-sensitive resistor 36 is increased at the moment, so that the magnetic force of the third electromagnet 38 is reduced, the magnetic attraction force to the fifth conductive block 33 is reduced, the contact position of the first conductive block 10 and the resistor coil 9 is unchanged due to the locking of the stop plate 40, so that the magnetic force of the second electromagnet 37 is unchanged, and the balance is broken at the moment, so that the fifth conductive block 33 slides towards the second electromagnet 37 until the fifth conductive block 33 is contacted with the sixth conductive block 35, thereby make first solenoid valve 29 circular telegram close, second solenoid valve 41 circular telegram is opened, and the reciprocating motion of pump plate 22 can pump the nutrient solution in the pump cistern 21 into third pipe fitting 30 this moment, and rethread third pipe fitting 30 pumps into in the culture dish 6, carries out the replenishment of nutrient solution, and automatic replenishment nutrient solution that can be timely guarantees the normal clear of cultivateing, and when the effectual opening device of having avoided carries out the replenishment of nutrient solution simultaneously, external bacterium and virus get into in the device.
It should be noted that when the nutrient solution is sufficient, the weight of the culture dish 6 makes the contact position of the first slider 4 and the resistor coil 9 make the magnetic force of the second electromagnet 37 consistent with the magnetic force of the third electromagnet 38 due to the resistance value of the force-sensitive resistor 36, so that the magnetic attraction forces on two sides of the fifth conductive block 33 are balanced, the fifth conductive block 33 is located at the middle position of the cavity 31, and the fifth conductive block 33 is not in contact with the sixth conductive block 35.
The control mechanism further comprises a force-sensitive resistor 36 fixedly embedded on the placing plate 5, the resistance value of the force-sensitive resistor 36 can be reduced along with the increase of the pressure born by the surface of the force-sensitive resistor 36, so that the force-sensitive resistor 36 can be changed according to the weight of the culture dish 6, the smaller the weight is, the larger the resistance value of the force-sensitive resistor 36 is, the force-sensitive resistor 36 is electrically connected with a third electromagnet 38 through a wire, two electric push rods 39 are fixedly connected to the inner bottom wall of the culture tank 2, the output end of each electric push rod 39 is fixedly connected with a stop plate 40, the electric push rods 39 can be electrified when the device is electrified, the stop plates 40 are in contact with the first sliding blocks 4, the positions of the first sliding blocks 4 are locked, and the follow-up perception of the nutrient solution in the culture dish 6 is facilitated.
Two second sliding grooves 18 are formed in the lower surface of the placement plate 5, a second sliding block 19 is connected to the second sliding groove 18 in a sliding mode, the second sliding block 19 is fixedly connected with the corresponding first sliding block 4, the sections of the second sliding groove 18 and the second sliding block 19 are T-shaped, the T-shaped second sliding block 19 and the second sliding groove 18 ensure that the placement plate 5 can slide relative to the first sliding block 4, and meanwhile stable connection can be guaranteed, and the placement plate 5 is prevented from falling off.
The upper surface of the placing plate 5 is fixedly embedded with a plurality of anti-slip pads 20, and the anti-slip pads 20 increase the friction force between the culture dish 6 and the placing plate 5, so that the placing plate 5 is guaranteed to vibrate together by driving the culture dish 6 through the friction force when vibrating left and right.
The first pipe fitting 27, the second pipe fitting 28 and the third pipe fitting 30 are respectively provided with a one-way valve, the one-way valve in the first pipe fitting 27 only allows nutrient solution to enter the pump liquid groove 21 from the liquid storage cavity 26, when the pump plate 22 slides towards the second gear 24, nutrient solution can be pumped into the pump liquid groove 21 through the first pipe fitting 27, the one-way valve in the second pipe fitting 28 only allows nutrient solution to enter the pump liquid groove 21 from the liquid storage cavity 26, so that when nutrient solution is not added, nutrient solution can be returned into the liquid storage cavity 26 from the second pipe fitting 28, and the one-way valve in the third pipe fitting 30 only allows nutrient solution to enter the third pipe fitting 30 from the pump liquid groove 21, so that nutrient solution replenishment can be carried out through the third pipe fitting 30.
The side wall of the stop plate 40, which is close to one side of the corresponding first sliding block 4, is fixedly connected with a rubber pad, and the rubber pad increases friction force between the stop plate 40 and the first sliding block 4, so that after the stop plate 40 abuts against the first sliding block 4, the position of the first sliding block 4 can be effectively locked.
In the invention, after the tissue culture experiment body is configured in the culture dish 6 and a sufficient amount of nutrient solution is added, the device is opened, the culture dish 6 is placed on the placement plate 5, at the moment, the placement plate 5 drives the first sliding block 4 to slide downwards by the gravity of the culture dish 6, so that the contact position of the first conductive block 10 and the resistance coil 9 is changed, the resistance of the first electromagnet 17 and the second electromagnet 37 is changed, after the placement plate 5 is stationary, the device is closed, the device is electrified, and the electric push rod 39 is electrified, so that the stop plate 40 is pushed by the electric push rod 39 to abut against the first sliding block 4, and then the position of the first sliding block 4 is locked;
simultaneously, the micro motor 12 is electrified and started, the micro motor 12 drives the first gear 13 to rotate, so that the first gear 13 drives the second conductive block 14 to rotate along with the first gear 13, the second conductive block 14 is alternately contacted with the third conductive block 15 and the fourth conductive block 16 under the rotation of the second conductive block 14, and the first electromagnets 17 on the left side and the right side are alternately electrified, so that the placing plate 5 is subjected to magnetic attraction in different alternating directions, and the placing plate 5 drives the culture dish 6 to continuously oscillate for culture;
when the oscillation frequency needs to be changed, the rotation speed of the micro motor 12 can be changed, so that the frequency of the second conductive block 14, the third conductive block 15 and the fourth conductive block 16 which are alternately contacted can be changed, that is, the alternating frequency of the alternating power-on of the first electromagnet 17 can be changed, that is, the reciprocating oscillation frequency of the placing plate 5 can be changed, and the oscillation frequency can be changed.
In the culture process, when the nutrient solution is sufficient, the resistance value of the force-sensitive resistor 36 enables the magnetic force generated by the third electromagnet 38 to be consistent with the magnetic force generated by the second electromagnet 37 at the contact position of the first conductive block 10 and the resistor coil 9, so that the magnetic attraction force borne by the fifth conductive block 33 is balanced and is positioned at the center of the cavity 31, the first electromagnetic valve 29 is powered off and opened, the second electromagnetic valve 41 is powered off and closed, and meanwhile, the first gear 13 drives the second gear 24 meshed with the first electromagnetic valve to rotate, and the second gear 24 drives the pump plate 22 to reciprocate through the connecting rod 25. Through the reciprocating motion of the pump plate 22, the nutrient solution in the liquid storage cavity 26 is pumped into the pump liquid groove 21 through the first pipe fitting 27, and then pumped back into the liquid storage cavity 26 through the second pipe fitting 28, so that the nutrient solution in the liquid storage cavity 26 keeps flowing continuously, and the uniform mixing of the nutrient solution is ensured at all times.
In the culture process, along with continuous consumption of nutrient solution in the culture dish 6, the weight of the culture dish 6 is continuously lightened, so that the resistance value of the force-sensitive resistor 36 is continuously increased, the resistance value of the force-sensitive resistor 36 is increased, namely, the magnetic force of a third electromagnet 38 connected with the force-sensitive resistor 36 is continuously reduced, the contact position of the first conductive block 10 and the resistor coil 9 is unchanged due to the locking of the stop plate 40, namely, the magnetic force of the second electromagnet 37 is unchanged, at the moment, the magnetic force born by the fifth conductive block 33 is not balanced any more, the magnetic force of the second electromagnet 37 is larger, the fifth conductive block 33 is enabled to slide in the second electromagnet 37 direction until the second electromagnet 37 is contacted with the sixth conductive block 35, the first electromagnetic valve 29 is electrified and closed, the second electromagnetic valve 41 is electrified and opened, at the moment, the nutrient solution in the liquid storage cavity 26 is pumped into the liquid storage groove 21 through the first pipe fitting 27, the nutrient solution is then pumped into the culture dish 6 through the third pipe fitting 30, the nutrient solution is replenished, along with continuous replenishment of the nutrient solution, the weight of the culture dish 6 is continuously increased, at the moment, the magnetic force born by the fifth conductive block 33 is not balanced, the magnetic force born by the fifth electromagnet 37 is not balanced again, the magnetic force born by the fifth electromagnet 33 is not consistent with the second electromagnetic valve 37, the magnetic force is recovered until the magnetic force of the second electromagnet is recovered to the second electromagnetic valve 35, the second electromagnetic valve is continuously recovered, the magnetic force is recovered, the magnetic force in the magnetic force is recovered to the original position is recovered, and the original magnetic force is recovered, and the magnetic force in the magnetic force is recovered, and the original magnetic force is recovered, and the magnetic force is recovered.
Claims (7)
1. The utility model provides an intelligent automatic culture device for tissue culture, includes device shell (1), its characterized in that, culture tank (2) have been seted up to device shell (1), two first sliding tray (3) have been seted up to device shell (1) diapire, sliding connection has first slider (4) in first sliding tray (3), and two first slider (4) joint sliding connection have place board (5), two second sliding tray (18) have been seted up to place board (5) lower surface, sliding connection has second slider (19) in second sliding tray (18), second slider (19) with corresponding first slider (4) fixed connection, the cross-section of second sliding tray (18) and second slider (19) is the T font, be provided with vibration mechanism in device shell (1);
the utility model provides a vibration mechanism is including seting up functional cavity (11) in device shell (1) diapire, roof fixedly connected with micro motor (12) in functional cavity (11), the output shaft interference fit of micro motor (12) has first gear (13), fixed embedding is equipped with second conducting block (14) under first gear (13), fixed embedding is equipped with a plurality of third conducting block (15), fourth conducting block (16) in functional cavity (11) inner wall, two inside walls that culture tank (2) are relative are all fixedly connected with first electro-magnet (17), third conducting block (15) and fourth conducting block (16) respectively with corresponding first electro-magnet (17) are connected through the wire, one of them mounting groove (8) have been seted up to first sliding groove (3) inside wall, mounting groove (8) inner wall is fixed with resistance coil (9), one of them first conducting block (10) are connected with to first sliding block (4) lower surface.
2. The intelligent automatic culture device for tissue culture according to claim 1, wherein a liquid pumping mechanism for supplying nutrient solution is arranged in the device housing (1), the liquid pumping mechanism comprises two liquid pumping grooves (21) which are formed in the device housing (1), the liquid pumping grooves (21) are communicated with the functional cavities (11), two cavities (26) are formed in the device housing (21) in a sealing sliding manner, two rotating rods (23) are rotatably connected to the inner bottom wall of the functional cavities (11) through bearings, a second gear (24) is in interference fit with the rotating rods (23), the second gear (24) is meshed with the first gear (13), a connecting rod (25) is rotatably connected to the upper surface of the second gear (24) through a pin shaft, one end of the connecting rod (25) far away from the second gear (24) is rotatably connected with the side wall of the corresponding pump plate (22) through the pin shaft, two cavities (26) are formed in the device housing (1), the liquid storage device housing (1) is fixedly connected with two first pipe fittings (27), the second pipe fittings (28) penetrate through the corresponding second pipe fittings (28) and the other ends of the first pipe fittings (28) penetrate through the corresponding pipe fittings (28), one end of the third pipe fitting (30) penetrates and extends into the corresponding pumping liquid groove (21), and the other end of the third pipe fitting penetrates and extends into the culture groove (2).
3. The intelligent automatic culture device for tissue culture according to claim 2, wherein a control mechanism for controlling a liquid pumping mechanism is arranged in the device housing (1), the control mechanism comprises a cavity (31) formed in the device housing (1), a second electromagnet (37) is fixedly connected to the side wall on one side of the cavity (31), a third electromagnet (38) is fixedly connected to the side wall on the other side of the cavity, the second electromagnet (37) is connected with the first electromagnet (17) through a wire, a third sliding groove (32) is formed in the inner top wall and the inner bottom wall of the cavity (31), a fifth conducting block (33) is connected in the third sliding groove (32) in a sliding manner, a plurality of second springs (34) are fixedly connected between the fifth conducting block (33) and the inner wall of the third sliding groove (32), a sixth conducting block (35) is fixedly embedded in the inner side wall of the cavity (31), a first electromagnetic valve (29) is arranged in the second pipe (28), a second electromagnetic valve (41) is arranged in the third pipe (30), and the fifth conducting block (33) and the second electromagnetic valve (29) are connected through the wire.
4. An intelligent automatic culture device for tissue culture according to claim 3, wherein the control mechanism further comprises a force-sensitive resistor (36) fixedly embedded on the upper surface of the placing plate (5), the force-sensitive resistor (36) is electrically connected with a third electromagnet (38) through a wire, two electric push rods (39) are fixedly connected to the inner bottom wall of the culture tank (2), and a stop plate (40) is fixedly connected to the output end of the electric push rods (39).
5. The intelligent automatic culture device for tissue culture according to claim 1, wherein a plurality of anti-slip pads (20) are fixedly embedded on the upper surface of the placement plate (5).
6. An intelligent automatic culture device for tissue culture according to claim 3, wherein the first pipe (27), the second pipe (28) and the third pipe (30) are respectively provided with a one-way valve.
7. The intelligent automatic tissue culture device according to claim 4, wherein a rubber pad is fixedly connected to a side wall of the stop plate (40) close to the corresponding first slider (4).
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| WO2013063128A1 (en) * | 2011-10-24 | 2013-05-02 | Bend Research, Inc. | Systems and methods for producing bioproducts |
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| CN205741061U (en) * | 2016-04-25 | 2016-11-30 | 高香红 | A kind of Tissue Culture Dish of replaceable culture fluid |
| WO2018229740A1 (en) * | 2017-06-15 | 2018-12-20 | 株式会社京都製作所 | Culture device, and culture method |
| CN111849751A (en) * | 2020-07-02 | 2020-10-30 | 宁波卫生职业技术学院 | Microorganism culture device and operation method |
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