CN114456934A - Intelligent automatic culture apparatus for tissue culture - Google Patents
Intelligent automatic culture apparatus for tissue culture Download PDFInfo
- Publication number
- CN114456934A CN114456934A CN202210197815.8A CN202210197815A CN114456934A CN 114456934 A CN114456934 A CN 114456934A CN 202210197815 A CN202210197815 A CN 202210197815A CN 114456934 A CN114456934 A CN 114456934A
- Authority
- CN
- China
- Prior art keywords
- sliding
- culture
- pipe fitting
- fixedly connected
- device shell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000015097 nutrients Nutrition 0.000 claims abstract description 53
- 239000007788 liquid Substances 0.000 claims description 34
- 238000003860 storage Methods 0.000 claims description 15
- 230000010355 oscillation Effects 0.000 claims description 12
- 238000005086 pumping Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 3
- 239000013589 supplement Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 44
- 239000012530 fluid Substances 0.000 description 13
- 238000000034 method Methods 0.000 description 8
- 239000011550 stock solution Substances 0.000 description 7
- 241000700605 Viruses Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000011124 ex vivo culture Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Computer Hardware Design (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an intelligent automatic culture device for tissue culture, which comprises a device shell, wherein the device shell is provided with a culture tank, the bottom wall of the device shell is provided with two first sliding grooves, the first sliding grooves are in sliding connection with first sliding blocks, the two first sliding blocks are jointly in sliding connection with a placing plate, and a vibrating mechanism is arranged in the device shell; vibrate mechanism including seting up the function chamber in the device shell diapire, function intracavity roof fixedly connected with micro motor, micro motor's output shaft interference fit has first gear, the fixed surface inlays and is equipped with the second conducting block under the first gear, the fixed embedding of function intracavity diapire is equipped with a plurality of third conducting blocks, fourth conducting block. Has the advantages that: the invention can intelligently and automatically adjust the vibration force, ensure the vibration effect, automatically supplement nutrient solution, and avoid the culture body from being polluted by substances such as external bacteria and the like without opening the device during supplement.
Description
Technical Field
The invention relates to the technical field of tissue culture, in particular to an intelligent automatic culture device for tissue culture.
Background
Tissue culture is one of the important means in biological research, and is also called ex vivo culture, which means to separate a desired tissue from a plant body. Organs or cells, protoplasts, etc., are cultured under artificially controlled conditions by aseptic manipulation to obtain regenerated whole plants or techniques for the production of other products of economic value which are commonly used in plant breeding, plant detoxification and rapid propagation, production of useful products of plants, preservation and exchange of plant germplasm resources. In the aspects of genetic, physiological, biochemical and pathological researches and the like, in the tissue culture process, a culture device is generally needed to be used for continuously shaking a culture dish, so that cells are deposited and accumulated to influence development and propagation.
Among the prior art, current culture apparatus's shaking force often is fixed, when to the tissue culture of not equidimension, the unable automatic adjustment of shaking force, shaking force too big can lead to the interior material of culture dish excessive, and shaking force undersize will lead to shaking unevenly, and it is inconvenient to use, simultaneously, in the process of cultivateing, often need the opening device to add the nutrient solution, in the opening device probably leads to external bacterium or virus to get into the culture dish, influences the experimental result.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, the oscillation force cannot be automatically adjusted, bacteria easily enter when equipment is opened to add nutrient solution, and the like, and provides an intelligent automatic culture device for tissue culture.
In order to achieve the purpose, the invention adopts the following technical scheme:
an intelligent automatic culture device for tissue culture comprises a device shell, wherein the device shell is provided with a culture groove, the bottom wall of the device shell is provided with two first sliding grooves, the first sliding grooves are in sliding connection with first sliding blocks, the two first sliding blocks are jointly in sliding connection with a placing plate, and a vibration mechanism is arranged in the device shell;
vibrate mechanism is including seting up the function chamber in the device shell diapire, function intracavity roof fixedly connected with micro motor, micro motor's output shaft interference fit has first gear, the fixed surface of first gear lower surface inlays and is equipped with the second conducting block, the fixed embedding of function intracavity diapire is equipped with a plurality of third conducting blocks, fourth conducting block, cultivate the equal fixedly connected with first electro-magnet of two inside walls that the groove is relative, third conducting block and fourth conducting block respectively with correspond first electro-magnet passes through the wire and is connected, one of them the mounting groove has been seted up to first sliding tray inside wall, the mounting groove inner wall is fixed with resistance coil, one of them the fixed surface of first slider lower surface is connected with first conducting block.
Further, a pump liquid mechanism for supplying nutrient solution is arranged in the device shell and comprises two pump liquid grooves formed in the device shell, the pump liquid grooves are communicated with the function cavity, a pump plate is connected in the pump liquid grooves in a sealing and sliding mode, the inner bottom wall of the function cavity is connected with two rotating rods through bearings in a rotating mode, the rotating rods are in interference fit with second gears, the second gears are meshed with the first gears, the upper surfaces of the second gears are connected with connecting rods through pin shafts in a rotating mode, one ends, far away from the second gears, of the connecting rods are connected with the corresponding side walls of the pump plate through pin shafts in a rotating mode, the device shell is provided with two liquid storage cavities, the device shell is fixedly connected with two first pipe fittings, a second pipe fitting and a third pipe fitting in a penetrating mode, one ends of the first pipe fittings and the second pipe fittings extend into the corresponding pump liquid grooves, the other end runs through and extends to corresponding stock solution chamber, third pipe fitting one end runs through and extends to corresponding pump liquid inslot, and the other end runs through and extends to in the culture tank.
Further, a control mechanism for controlling the liquid pumping mechanism is arranged in the device shell, the control mechanism comprises a cavity arranged in the device shell, the side wall of one side of the cavity is fixedly connected with a second electromagnet, the side wall of the other side is fixedly connected with a third electromagnet, the second electromagnet is connected with the first electromagnet through a lead, third sliding grooves are respectively arranged on the inner top wall and the inner bottom wall of the cavity, a fifth conductive block is connected in the third sliding groove in a sliding manner, a plurality of second springs are fixedly connected between the fifth conductive block and the inner wall of the third sliding groove, a sixth conductive block is fixedly embedded in the inner side wall of the cavity, a first electromagnetic valve is arranged in the second pipe fitting, and a second electromagnetic valve is arranged in the third pipe fitting, and the fifth conductive block, the sixth conductive block, the first electromagnetic valve and the second electromagnetic valve are connected through leads.
Further, control mechanism still includes the fixed quick resistance of establishing on placing the board upper surface of inlaying, quick resistance of force passes through the wire electricity with the third electro-magnet and is connected, two electric putter of diapire fixedly connected with in the culture tank, electric putter's output fixedly connected with locking plate.
Furthermore, two second sliding grooves are formed in the lower surface of the placing plate, second sliding blocks are connected in the second sliding grooves in a sliding mode, the second sliding blocks are fixedly connected with the corresponding first sliding blocks, and the cross sections of the second sliding grooves and the second sliding blocks are T-shaped.
Furthermore, a plurality of anti-slip mats are fixedly embedded on the upper surface of the placing plate.
Furthermore, check valves are arranged in the first pipe fitting, the second pipe fitting and the third pipe fitting.
Furthermore, a rubber pad is fixedly connected to the side wall of the stop plate close to one side of the corresponding first sliding block.
The invention has the following advantages:
1. during culture, the culture dish is placed on the placing plate, the placing plate slides downwards in different degrees according to the total mass of the culture dish, so that the contact position of the first conductive block and the resistance coil is changed, the resistance connected to the first electromagnet is changed, the magnetic force of the first electromagnet is changed, the two first electromagnets are alternately electrified by matching with the rotation of the micro motor, the placing plate drives the culture dish to vibrate, the vibration force can be automatically adjusted according to the weight of the culture dish, and the phenomenon that substances in the culture dish overflow due to the fact that the vibration force is too large and the vibration is not uniform due to the fact that the vibration force is too small is effectively avoided;
2. in the culture process, after the culture plate is electrified, the electric push rod locks the position of the first slide block, meanwhile, the weight of the culture dish is sensed through the force sensitive resistor, meanwhile, the position of the first slide block determines the magnetic force of the second electromagnet, the magnetic force of the second electromagnet is not changed due to position locking, the force sensitive resistor determines the magnetic force of the third electromagnet, the magnetic force changes along with the change of the weight of the culture dish, initially, the magnetic force of the third electromagnet is consistent with that of the second electromagnet, when nutrient solution is insufficient, the magnetic force of the third electromagnet is reduced, so that the fifth conductive block is contacted with the sixth conductive block, the two electromagnetic valves are electrified, at the moment, the reciprocating motion of the pump plate can automatically supply the nutrient solution, the nutrient solution can be automatically supplied in time, and the normal culture is ensured;
3. the nutrient solution is automatically supplemented through the pump plate, the opening device is not required to be opened for adding the nutrient solution, and the problem that when the opening device is used for supplementing the nutrient solution, external bacteria and viruses enter the device to influence the experimental result of tissue culture is effectively avoided;
4. when the nutrient solution is not required to be supplemented, the first electromagnetic valve is opened, the second electromagnetic valve is closed, the reciprocating motion of the pump plate can pump the nutrient solution in the liquid storage cavity into the pump liquid tank through the first pipe fitting, and then the nutrient solution in the liquid storage cavity is pumped back to the liquid storage cavity through the second pipe fitting, so that the nutrient solution in the liquid storage cavity keeps flowing continuously, and the uniform mixing of the nutrient solution is ensured constantly.
Drawings
FIG. 1 is a schematic structural diagram of an intelligent automatic culture apparatus for tissue culture according to the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1;
FIG. 3 is an enlarged view taken at A in FIG. 1;
FIG. 4 is an enlarged view of FIG. 1 at B;
FIG. 5 is an enlarged view at C of FIG. 1;
FIG. 6 is a partial cross-sectional view taken at D-D of FIG. 1;
FIG. 7 is a partial cross-sectional view taken at E-E of FIG. 1;
FIG. 8 is a schematic circuit diagram of an intelligent automatic culture apparatus for tissue culture according to the present invention.
In the figure: the device comprises a device shell, a culture tank 2, a first sliding groove 3, a first sliding block 4, a placing plate 5, a culture dish 6, a first spring 7, a mounting groove 8, a resistance coil 9, a first conductive block 10, a functional cavity 11, a micro motor 12, a first gear 13, a second conductive block 14, a third conductive block 15, a fourth conductive block 16, a first electromagnet 17, a second sliding groove 18, a second sliding block 19, a non-slip mat 20, a pump liquid tank 21, a pump plate 22, a rotating rod 23, a second gear 24, a connecting rod 25, a liquid storage cavity 26, a first pipe 27, a second pipe 28, a first electromagnetic valve 29, a third pipe 30, a cavity 31, a third sliding groove 32, a fifth conductive block 33, a second spring 34, a sixth conductive block 35, a force sensitive resistor 36, a second electromagnet 37, a third electromagnetic valve 38, an electric push rod 39, an electromagnetic stop plate 40 and a second electromagnetic valve 41.
Detailed Description
Referring to fig. 1-8, an intelligent automatic culture device for tissue culture comprises a device shell 1, wherein the device shell 1 is connected with a device cover through a hinge, the device shell 1 is provided with a culture tank 2, the bottom wall of the device shell 1 is provided with two first sliding grooves 3, the first sliding grooves 3 are connected with first sliding blocks 4 in a sliding manner, the two first sliding blocks 4 are jointly connected with a placing plate 5 in a sliding manner, the placing plate 5 is made of metal, and a vibration mechanism is arranged in the device shell 1;
the oscillating mechanism comprises a function cavity 11 arranged in the bottom wall of the device shell 1, a micro motor 12 is fixedly connected to the top wall in the function cavity 11, a first gear 13 is in interference fit with an output shaft of the micro motor 12, a second conductive block 14 is fixedly embedded on the lower surface of the first gear 13, an insulating coating is coated between the first gear 13 and the second conductive block 14 so as to prevent the first gear 13 and the second conductive block 14 from being communicated, a plurality of third conductive blocks 15 and fourth conductive blocks 16 are fixedly embedded on the bottom wall in the function cavity 11, as shown in figure 7, the third conductive blocks 15 and the fourth conductive blocks 16 are alternately arranged, so that the micro motor 12 drives the first gear 13 to rotate, the second conductive blocks 14 are constantly and alternately contacted with the third conductive blocks 15 and the fourth conductive blocks 16 in the rotating process of the first gear 13, two opposite inner side walls of the culture tank 2 are both fixedly connected with first electromagnets 17, as shown in fig. 1, the left and right inner side walls are fixedly connected with a first electromagnet 17, the third conductive block 15 and the fourth conductive block 16 are respectively connected with the corresponding first electromagnets 17 through wires, the third conductive block 15 is connected with the left first electromagnet 17, the fourth conductive block 16 is connected with the right first electromagnet 17, so that the second conductive block 14 alternately contacts with the third conductive block 15 and the fourth conductive block 16, the two first electromagnets 17 are alternately energized, the placing plate 5 is continuously oscillated by the magnetic force generated alternately, the culture dish 6 is driven to oscillate, wherein an inner side wall of one first sliding groove 3 is provided with a mounting groove 8, the inner wall of the mounting groove 8 is fixedly provided with a resistance coil 9, the lower surface of one first sliding block 4 is fixedly connected with a first conductive block 10, the circuit connection relationship is as shown in fig. 8, the resistance coil 9 is connected with one pole of a power supply, the second conductive block 14 is connected with the first conductive block 10, the second electromagnet 37, the first electromagnet 17, the third conductive block 15 and the fourth conductive block 16 are connected with the other pole of the power supply, so that the first conductive block 10 can slide downwards while the contact position between the first conductive block 10 and the resistance coil 9 can be changed, when the culture dish 6 is heavier, the sliding position of the first conductive block 10 is larger, so that the resistance value connected to the first electromagnet 17 is reduced, so that the magnetic force of the first electromagnet 17 is increased, namely the magnetic attraction force of the first electromagnet 17 to the placing plate 5 is increased, similarly, when the culture dish 6 is lighter, the magnetic attraction force of the first electromagnet 17 to the placing plate 5 is reduced, so that the oscillation force of the placing plate 5 can be automatically adjusted according to the weight of the culture dish 6, thereby effectively avoiding the phenomenon that the substance in the culture dish 6 overflows due to overlarge oscillation force, the too small oscillation force leads to uneven oscillation, so that the device is more intelligent and automatic.
It should be noted that when the oscillation frequency needs to be changed, the rotation speed of the micro motor 12 can be changed, so as to change the frequency of the second conductive block 14 contacting with the third conductive block 15 and the fourth conductive block 16 alternately, that is, the alternating frequency of the first electromagnet 17 conducting alternately can be changed, that is, the reciprocating oscillation frequency of the placing plate 5 can be changed, thereby changing the oscillation frequency.
A pump liquid mechanism for supplying nutrient solution is arranged in the device shell 1, the pump liquid mechanism comprises two pump liquid grooves 21 arranged in the device shell 1, the pump liquid grooves 21 are communicated with the function cavity 11, a pump plate 22 is connected in the pump liquid grooves 21 in a sealing and sliding manner, two rotating rods 23 are rotatably connected to the inner bottom wall of the function cavity 11 through bearings, the rotating rods 23 are in interference fit with second gears 24, the second gears 24 are meshed with the first gears 13, the upper surfaces of the second gears 24 are rotatably connected with connecting rods 25 through pin shafts, one ends of the connecting rods 25 far away from the second gears 24 are rotatably connected with the corresponding side walls of the pump plate 22 through pin shafts, the device shell 1 is provided with two liquid storage cavities 26, the device shell 1 is fixedly connected with two first pipe fittings 27, second pipe fittings 28 and third pipe fittings 30 in a penetrating manner, one ends of the first pipe fittings 27 and the second pipe fittings 28 extend into the corresponding pump liquid grooves 21, and the other ends extend into the corresponding liquid storage cavities 26, one end of the third pipe 30 extends into the corresponding pump fluid tank 21 and the other end extends into the culture tank 2, as shown in fig. 1, the length of the first pipe 27 is greater than that of the second pipe 28, so that the first pipe 27 extends into the bottom of the nutrient fluid in the fluid storage cavity 26, and the second pipe 28 does not extend into the nutrient fluid, so that the first gear 13 rotates and simultaneously drives the second gear 24 engaged with the first gear to rotate, so that the second gear 24 drives the pump plate 22 to reciprocate through the connecting rod 25, and through the control of the control mechanism, when the nutrient fluid is not supplemented, the reciprocating motion of the pump plate 22 can pump the nutrient fluid in the fluid storage cavity 26 into the pump fluid tank 21 through the first pipe 27 and then pump the nutrient fluid back into the fluid storage cavity 26 through the second pipe 28, so that the nutrient fluid in the fluid storage cavity 26 keeps flowing constantly, thereby constantly ensuring the uniform mixing of the nutrient fluid, when the nutrient solution is supplemented, the nutrient solution can be supplemented into the culture dish 6 through the third pipe member 30.
The device shell 1 is internally provided with a control mechanism for controlling a liquid pumping mechanism, the control mechanism comprises a cavity 31 arranged in the device shell 1, the side wall of one side of the cavity 31 is fixedly connected with a second electromagnet 37, the side wall of the other side of the cavity is fixedly connected with a third electromagnet 38, the second electromagnet 37 is connected with the first electromagnet 17 through a lead, the top wall and the inner bottom wall of the cavity 31 are both provided with a third sliding groove 32, the third sliding groove 32 is internally and slidably connected with a fifth conducting block 33, a plurality of second springs 34 are fixedly connected between the fifth conducting block 33 and the inner wall of the third sliding groove 32, the inner side wall of the cavity 31 is fixedly embedded with a sixth conducting block 35, a first electromagnetic valve 29 is arranged in the second pipe fitting 28, the first electromagnetic valve 29 is a normally open electromagnetic valve and is closed when being electrified, a second electromagnetic valve 41 is arranged in the third pipe fitting 30, the second electromagnetic valve 41 is a normally closed electromagnetic valve and is opened when being electrified, the fifth conductive block 33, the sixth conductive block 35, the first electromagnetic valve 29 and the second electromagnetic valve 41 are connected through conducting wires, after the fifth conductive block 33 is contacted with the sixth conductive block 35, the first electromagnetic valve 29 and the second electromagnetic valve 41 can be electrified, in the culture process, the force-sensitive resistor 36 senses the gravity of the culture dish 6 in real time, when the nutrient solution in the culture dish 6 is reduced to a certain degree, the resistance value of the force-sensitive resistor 36 is increased, the magnetic force of the third electromagnet 38 is reduced, the magnetic attraction force to the fifth conductive block 33 is reduced, due to the locking of the stop plate 40 on the first sliding block 4, the contact position of the first conductive block 10 and the resistance coil 9 is unchanged, the magnetic force of the second electromagnet 37 is unchanged, the balance is broken at this time, the fifth conductive block 33 slides towards the second electromagnet 37 until the fifth conductive block 33 is contacted with the sixth conductive block 35, thereby make first solenoid valve 29 circular telegram close, second solenoid valve 41 circular telegram is opened, and the reciprocating motion of pump plate 22 can be with the nutrient solution pump income third pipe fitting 30 in the pump cistern 21 this moment, and rethread third pipe fitting 30 pump goes into in the culture dish 6, carries out the replenishment of nutrient solution, and automatic replenishment nutrient solution that can be timely guarantees the normal clear of cultivateing, and when simultaneously effectual opening device carried out the replenishment of nutrient solution, in external bacterium and the virus access device.
It is worth mentioning that, when the nutrient solution is sufficient, the position of the culture dish 6, where the first sliding block 4 contacts the resistance coil 9, enables the magnetic force of the second electromagnet 37 to be consistent with the resistance of the force sensitive resistor 36, so that the magnetic force of the third electromagnet 38 is balanced, the fifth conducting block 33 is located in the middle of the cavity 31, and the fifth conducting block 33 does not contact with the sixth conducting block 35.
The control mechanism further comprises a force sensitive resistor 36 fixedly embedded on the upper surface of the placing plate 5, the resistance of the force sensitive resistor 36 can be reduced along with the increase of the pressure on the surface of the force sensitive resistor 36, so that the force sensitive resistor 36 can be changed according to the weight of the culture dish 6, the smaller the weight is, the larger the resistance of the force sensitive resistor 36 is, the force sensitive resistor 36 is electrically connected with a third electromagnet 38 through a lead, two electric push rods 39 are fixedly connected to the bottom wall of the culture groove 2, a stop plate 40 is fixedly connected to the output end of each electric push rod 39, the electric push rods 39 can be powered on while the device is powered on, so that the stop plate 40 is in contact with the first sliding block 4, the position of the first sliding block 4 is locked, and the quantity of nutrient solution in the culture dish 6 can be sensed later.
Two second sliding grooves 18 are formed in the lower surface of the placing plate 5, a second sliding block 19 is connected in the second sliding grooves 18 in a sliding mode, the second sliding block 19 is fixedly connected with the corresponding first sliding block 4, the cross sections of the second sliding grooves 18 and the second sliding block 19 are in a T shape, the second sliding block 19 and the second sliding grooves 18 in the T shape guarantee that the placing plate 5 can slide relative to the first sliding block 4, the two can also guarantee stable connection, and the placing plate 5 is prevented from falling off.
Place 5 fixed surface of board and inlay and be equipped with a plurality of slipmats 20, slipmat 20 increases culture dish 6 and places the frictional force between the board 5 to when guaranteeing to place board 5 and vibrate about, can drive culture dish 6 and vibrate together through frictional force.
First pipe fitting 27, second pipe fitting 28, all be provided with the check valve in the third pipe fitting 30, the check valve in the first pipe fitting 27 only allows the nutrient solution to get into pump cistern 21 by stock solution chamber 26, when guaranteeing that pump plate 22 slides to second gear 24 direction, can be through first pipe fitting 27 with nutrient solution suction pump cistern 21 in, the check valve in the second pipe fitting 28 only allows the nutrient solution to get into pump cistern 21 from stock solution chamber 26, thereby when guaranteeing not to add the nutrient solution, the nutrient solution can return in stock solution chamber 26 from second pipe fitting 28, the check valve in the third pipe fitting 30 only allows the nutrient solution to get into in the third pipe fitting 30 by pump cistern 21, thereby guarantee to carry out the replenishment of nutrient solution through third pipe fitting 30.
The side wall of the stop plate 40 close to the corresponding side of the first sliding block 4 is fixedly connected with a rubber pad, and the rubber pad increases the friction force between the stop plate 40 and the first sliding block 4, so that the position of the first sliding block 4 can be effectively locked after the stop plate 40 abuts against the first sliding block 4.
According to the invention, after the tissue culture experimental body is completely configured in the culture dish 6 and sufficient nutrient solution is added, the device is opened, the culture dish 6 is placed on the placing plate 5, at the moment, the placing plate 5 is driven by the gravity of the culture dish 6 to drive the first sliding block 4 to slide downwards, so that the contact position of the first conductive block 10 and the resistance coil 9 is changed, the resistance size of the first electromagnet 17 and the second electromagnet 37 is changed, after the placing plate 5 is static, the device is closed, the device is electrified, and the electric push rod 39 is electrified while the electric push rod is electrified, so that the position of the first sliding block 4 is locked after the stop plate 40 is pushed by the electric push rod 39 and abuts against the first sliding block 4;
meanwhile, the micro motor 12 is powered on and started, the micro motor 12 drives the first gear 13 to rotate, so that the first gear 13 drives the second conductive block 14 to rotate along with the first gear 13, and under the rotation of the second conductive block 14, the second conductive block 14 is alternately contacted with the third conductive block 15 and the fourth conductive block 16, so that the first electromagnets 17 on the left side and the right side are alternately powered on, so that the placing plate 5 is subjected to alternate magnetic attraction in different directions, and the placing plate 5 drives the culture dish 6 to continuously vibrate for culture;
when the oscillation frequency needs to be changed, the rotation speed of the micro motor 12 can be changed, so that the frequency of the second conductive block 14 in alternate contact with the third conductive block 15 and the fourth conductive block 16 is changed, that is, the alternate frequency of the first electromagnet 17 in alternate energization can be changed, that is, the reciprocating oscillation frequency of the placing plate 5 can be changed, and the oscillation frequency can be changed.
In the process of cultivateing, when the nutrient solution is sufficient, force sensing resistor 36's resistance makes the magnetic force that third electro-magnet 38 produced unanimous with first conducting block 10 and resistance coil 9 contact position messenger second electro-magnet 37 produced this moment to make the magnetic attraction that fifth conducting block 33 received balanced, make it be located the central point of cavity 31, first solenoid valve 29 outage is opened this moment, second solenoid valve 41 outage is closed, and simultaneously, first gear 13 drives the second gear 24 rotation rather than the meshing, makes second gear 24 drive pump plate 22 reciprocating motion through connecting rod 25. Through the reciprocating motion of pump plate 22, in the nutrient solution suction pump cistern 21 in with stock solution chamber 26 through first pipe fitting 27, in rethread second pipe fitting 28 pump back stock solution chamber 26 to make the nutrient solution in the stock solution chamber 26 keep continuous flow, thereby guarantee the misce bene of nutrient solution constantly.
In the culture process, with the continuous consumption of the nutrient solution in the culture dish 6, the weight of the culture dish 6 is continuously reduced, so that the resistance value of the force-sensitive resistor 36 is continuously increased, namely the resistance value of the force-sensitive resistor 36 is increased, namely the magnetic force of the third electromagnet 38 connected with the force-sensitive resistor 36 is continuously reduced, due to the locking of the stop plate 40, the contact position of the first conductive block 10 and the resistance coil 9 is unchanged, namely the magnetic force of the second electromagnet 37 is unchanged, at the moment, the magnetic force borne by the fifth conductive block 33 is not balanced, the magnetic force borne by the second electromagnet 37 is larger, the fifth conductive block 33 slides in the direction of the second electromagnet 37 until the fifth conductive block 35 is contacted, so that the first electromagnetic valve 29 is powered on and closed, the second electromagnetic valve 41 is powered on and opened, at the moment, the reciprocating motion of the pump plate 22 pumps the nutrient solution in the liquid storage cavity 26 into the pump liquid groove 21 through the first pipe fitting 27, and then pumps the nutrient solution into the culture dish 6 through the third pipe fitting 30, the nutrient solution is supplemented, the weight of the culture dish 6 is continuously increased along with the continuous supplement of the nutrient solution until the resistance value of the force sensitive resistor 36 is increased again to restore to the original resistance value, the magnetic force of the second electromagnet 37 is consistent with that of the third electromagnet 38 again, under the elastic force of the second spring 34, the fifth conductive block 33 restores to the middle position and is separated from the sixth conductive block 35, so that the first electromagnetic valve 29 and the second electromagnetic valve 41 are powered off, the nutrient solution is not supplemented any more by the reciprocating motion of the pump plate 22, the nutrient solution is continuously mixed until the nutrient solution is insufficient again, and the reciprocating motion is carried out so as to reach the specified culture time.
Claims (8)
1. The intelligent automatic culture device for tissue culture comprises a device shell (1) and is characterized in that a culture groove (2) is formed in the device shell (1), two first sliding grooves (3) are formed in the bottom wall of the device shell (1), a first sliding block (4) is connected in the first sliding grooves (3) in a sliding mode, a placing plate (5) is connected in the two first sliding blocks (4) in a sliding mode, and a vibrating mechanism is arranged in the device shell (1);
the oscillation mechanism comprises a function cavity (11) arranged in the bottom wall of the device shell (1), a micro motor (12) is fixedly connected to the inner top wall of the function cavity (11), a first gear (13) is in interference fit with an output shaft of the micro motor (12), a second conductive block (14) is fixedly embedded in the lower surface of the first gear (13), a plurality of third conductive blocks (15) and fourth conductive blocks (16) are fixedly embedded in the inner bottom wall of the function cavity (11), two opposite inner side walls of the culture tank (2) are fixedly connected with first electromagnets (17), the third conductive blocks (15) and the fourth conductive blocks (16) are respectively connected with the corresponding first electromagnets (17) through wires, one of the inner side walls of the first sliding groove (3) is provided with a mounting groove (8), and a resistance coil (9) is fixed to the inner wall of the mounting groove (8), the lower surface of one of the first sliding blocks (4) is fixedly connected with a first conductive block (10).
2. The intelligent automatic culture device for tissue culture according to claim 1, wherein a pump liquid mechanism for nutrient solution supply is arranged in the device housing (1), the pump liquid mechanism comprises two pump liquid tanks (21) arranged in the device housing (1), the pump liquid tanks (21) are communicated with the function cavity (11), a pump plate (22) is connected in the pump liquid tanks (21) in a sealing and sliding manner, two rotating rods (23) are rotatably connected to the bottom wall of the function cavity (11) through bearings, the rotating rods (23) are in interference fit with a second gear (24), the second gear (24) is meshed with the first gear (13), the upper surface of the second gear (24) is rotatably connected with a connecting rod (25) through a pin shaft, one end of the connecting rod (25) far away from the second gear (24) is rotatably connected with the corresponding side wall of the pump plate (22) through a pin shaft, two liquid storage cavities (26) have been seted up in device shell (1), device shell (1) runs through two first pipe fittings (27), second pipe fitting (28), third pipe fitting (30) of fixedly connected with, first pipe fitting (27), second pipe fitting (28) one end run through extend to correspond in pump cistern (21), the other end runs through extend to correspond liquid storage cavities (26), third pipe fitting (30) one end runs through extend to in corresponding pump cistern (21), the other end runs through extend to in culture tank (2).
3. The intelligent automatic culture device for tissue culture according to claim 2, wherein a control mechanism for controlling the liquid pumping mechanism is arranged in the device housing (1), the control mechanism comprises a cavity (31) arranged in the device housing (1), a second electromagnet (37) is fixedly connected to the side wall of one side of the cavity (31), a third electromagnet (38) is fixedly connected to the side wall of the other side of the cavity, the second electromagnet (37) is connected with the first electromagnet (17) through a wire, third sliding grooves (32) are respectively formed in the inner top wall and the inner bottom wall of the cavity (31), a fifth conductive block (33) is slidably connected in the third sliding groove (32), a plurality of second springs (34) are fixedly connected between the fifth conductive block (33) and the inner wall of the third sliding groove (32), and a sixth conductive block (35) is fixedly embedded in the inner side wall of the cavity (31), a first electromagnetic valve (29) is arranged in the second pipe fitting (28), a second electromagnetic valve (41) is arranged in the third pipe fitting (30), and the fifth conductive block (33), the sixth conductive block (35), the first electromagnetic valve (29) and the second electromagnetic valve (41) are connected through conducting wires.
4. The intelligent automatic culture device for tissue culture is characterized in that the control mechanism further comprises a force-sensitive resistor (36) fixedly embedded on the upper surface of the placing plate (5), the force-sensitive resistor (36) is electrically connected with the third electromagnet (38) through a lead, two electric push rods (39) are fixedly connected to the inner bottom wall of the culture tank (2), and a stop plate (40) is fixedly connected to the output ends of the electric push rods (39).
5. The intelligent automatic culture device for tissue culture according to claim 1, wherein two second sliding grooves (18) are formed in the lower surface of the placing plate (5), second sliding blocks (19) are connected in the second sliding grooves (18) in a sliding manner, the second sliding blocks (19) are fixedly connected with the corresponding first sliding blocks (4), and the sections of the second sliding grooves (18) and the second sliding blocks (19) are both T-shaped.
6. The intelligent automatic culture device for tissue culture is characterized in that a plurality of anti-skid pads (20) are fixedly embedded on the upper surface of the placing plate (5).
7. The intelligent automatic culture device for tissue culture is characterized in that the first pipe fitting (27), the second pipe fitting (28) and the third pipe fitting (30) are internally provided with one-way valves.
8. The intelligent automatic culture device for tissue culture is characterized in that a rubber pad is fixedly connected to the side wall of the stop plate (40) close to the corresponding side of the first sliding block (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210197815.8A CN114456934B (en) | 2022-03-02 | 2022-03-02 | Intelligent automatic culture device for tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210197815.8A CN114456934B (en) | 2022-03-02 | 2022-03-02 | Intelligent automatic culture device for tissue culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114456934A true CN114456934A (en) | 2022-05-10 |
| CN114456934B CN114456934B (en) | 2024-01-26 |
Family
ID=81414699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210197815.8A Active CN114456934B (en) | 2022-03-02 | 2022-03-02 | Intelligent automatic culture device for tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114456934B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114472007A (en) * | 2021-12-27 | 2022-05-13 | 张淑芬 | Electronic components's processing equipment for semiconductor chip |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140295532A1 (en) * | 2011-10-24 | 2014-10-02 | Bend Research, Inc. | Systems and methods for producing bioproducts |
| CN205741061U (en) * | 2016-04-25 | 2016-11-30 | 高香红 | A kind of Tissue Culture Dish of replaceable culture fluid |
| WO2018229740A1 (en) * | 2017-06-15 | 2018-12-20 | 株式会社京都製作所 | Culture device, and culture method |
| CN111849751A (en) * | 2020-07-02 | 2020-10-30 | 宁波卫生职业技术学院 | Microorganism culture device and operation method |
| CN112237872A (en) * | 2020-09-27 | 2021-01-19 | 广州丽都生物科技有限公司 | Biological cell culture is with shaking mixing device |
| CN112375679A (en) * | 2020-11-17 | 2021-02-19 | 苏州大学附属第二医院 | Cell periodic pressure electric applying device and applying method |
-
2022
- 2022-03-02 CN CN202210197815.8A patent/CN114456934B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140295532A1 (en) * | 2011-10-24 | 2014-10-02 | Bend Research, Inc. | Systems and methods for producing bioproducts |
| CN205741061U (en) * | 2016-04-25 | 2016-11-30 | 高香红 | A kind of Tissue Culture Dish of replaceable culture fluid |
| WO2018229740A1 (en) * | 2017-06-15 | 2018-12-20 | 株式会社京都製作所 | Culture device, and culture method |
| CN111849751A (en) * | 2020-07-02 | 2020-10-30 | 宁波卫生职业技术学院 | Microorganism culture device and operation method |
| CN112237872A (en) * | 2020-09-27 | 2021-01-19 | 广州丽都生物科技有限公司 | Biological cell culture is with shaking mixing device |
| CN112375679A (en) * | 2020-11-17 | 2021-02-19 | 苏州大学附属第二医院 | Cell periodic pressure electric applying device and applying method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114472007A (en) * | 2021-12-27 | 2022-05-13 | 张淑芬 | Electronic components's processing equipment for semiconductor chip |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114456934B (en) | 2024-01-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114456934A (en) | Intelligent automatic culture apparatus for tissue culture | |
| US4786240A (en) | Pumping apparatus with an electromagnet affixed to the septum | |
| US10845111B2 (en) | Layering of low thermal conductive material on metal tray | |
| US9759472B2 (en) | Clear ice maker with warm air flow | |
| JP3186772B2 (en) | Refrigerator water distributor | |
| US7378271B2 (en) | Device for pressurized perfusion especially for culturing and/or treating cells | |
| US20140165611A1 (en) | Clear ice maker | |
| US9410723B2 (en) | Ice maker with rocking cold plate | |
| WO2008061179A2 (en) | Devices and methods for making ice | |
| EP2743610A2 (en) | Clear ice maker and method for forming clear ice | |
| JP2652645B2 (en) | Ferrofluid pump for pumping biological fluid | |
| US5108552A (en) | Electroplating process | |
| EP2743613A2 (en) | Clear ice maker with varied thermal conductivity | |
| CN114326847B (en) | Intelligent pH value regulating and controlling device and method for protein modification research | |
| US3216650A (en) | Liquid aerator or pump | |
| CN209058484U (en) | A kind of intelligent modularized honeycomb couch | |
| CN113083135A (en) | Mixing equipment for easily precipitating chemical agent preparation | |
| CN2463759Y (en) | Full-automatic ice producer | |
| CN210842623U (en) | Cooking machine | |
| CN116411331B (en) | Coating device for magnet manufacturing | |
| CN219153498U (en) | Latex glove dipping device | |
| JP2001304452A (en) | Magnetic operating valve | |
| CN219101616U (en) | Pump, humidifier and fish tank | |
| CN215022097U (en) | Partitioned fumigating bed | |
| CN216602380U (en) | Tea basket lifting mechanism and teapot thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20240102 Address after: 201400, Buildings 6, 22, and 28, No. 356 Zhengbo Road, Lingang New Area, China (Shanghai) Pilot Free Trade Zone, Fengxian District, Shanghai Applicant after: Shanghai Bibo Biomedical Engineering Co.,Ltd. Address before: 226300 No. 107, Wanhe Runyuan shop, Jinxin street, Tongzhou District, Nantong City, Jiangsu Province Applicant before: Zou Yuliang |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |