CN205741061U - A kind of Tissue Culture Dish of replaceable culture fluid - Google Patents
A kind of Tissue Culture Dish of replaceable culture fluid Download PDFInfo
- Publication number
- CN205741061U CN205741061U CN201620349167.3U CN201620349167U CN205741061U CN 205741061 U CN205741061 U CN 205741061U CN 201620349167 U CN201620349167 U CN 201620349167U CN 205741061 U CN205741061 U CN 205741061U
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- Prior art keywords
- culture fluid
- culture
- fluid
- plug
- holds
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- 239000012531 culture fluid Substances 0.000 title claims abstract description 103
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000012530 fluid Substances 0.000 claims abstract description 12
- 229910052742 iron Inorganic materials 0.000 claims abstract description 10
- 238000009434 installation Methods 0.000 claims abstract description 6
- 239000002699 waste material Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 7
- 238000009423 ventilation Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 230000000740 bleeding effect Effects 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims 1
- 238000013461 design Methods 0.000 abstract description 6
- 238000010276 construction Methods 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 description 6
- 238000012549 training Methods 0.000 description 6
- 239000012930 cell culture fluid Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000005418 vegetable material Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
This utility model discloses the Tissue Culture Dish of a kind of replaceable culture fluid, leakage fluid dram is processed on the bottom surface of culture dish body, bleed plug is arranged in leakage fluid dram, the bottom fixed installation soft iron block of bleed plug, the bottom of described bleed plug is fixed on culture fluid by spring and holds on the bottom surface of intracavity, the right side that culture fluid holds bottom chamber arranges power source cavity, discharge opeing electromagnetic core is fixedly mounted on culture fluid and holds the central authorities of chamber exterior bottom, culture fluid adding tube is arranged on the central authorities of sealing-plug, culture fluid adder is arranged on the top of culture fluid adding tube, culture fluid sprinkler head is arranged on the bottom of culture fluid adding tube, culture fluid adds piston and is arranged in culture fluid adder, culture fluid adds bar and is arranged on culture fluid interpolation piston.This utility model uses culture fluid adder and the design of discharge opeing electromagnetic core, reaches to realize the discharge of culture fluid and the purpose of interpolation under conditions of being not switched on culture dish, and its design science is reasonable, and simple in construction is easy to use.
Description
Technical field:
This utility model relates to the Tissue Culture Dish of a kind of replaceable culture fluid, belongs to Tissue Culture Dish
Technical field.
Background technology:
Culture dish is a kind of labware cultivated for microorganism or cell, by a flat circle
The end of plate-like and a lid composition, typically make with glass or plastics.Culture dish material is substantially divided
It is two classes, mainly plastic and glass.The cultivation that may be used for vegetable material and microorganism of glass,
The adhere-wall culture of zooblast also available to.The mainly polythene material of plastics, once property makes
With with muptiple-use use two kinds, be suitable for laboratory inoculation, line, separation of bacterial etc. and operate, it is also possible to
Cultivation for vegetable material.
Existing culture dish, when carrying out cell and cultivating, adds and old culture fluid for new culture fluid
Discharging, it is necessary to open culture dish lid in aseptic operating platform and operate, this operating process bothers,
Time-consuming the longest and be easily caused cell contamination, affect the cultivation of cell training quality the most whole cell
Experiment process.
Utility model content:
The technical problems to be solved in the utility model is to provide the cell of a kind of replaceable culture fluid and cultivates
Ware, uses culture fluid adder and the design of discharge opeing electromagnetic core, reaches be not switched on culture dish
Under the conditions of realize the discharge of culture fluid and the purpose of interpolation, its design science is reasonable, simple in construction,
Easy to use.
The Tissue Culture Dish of a kind of replaceable culture fluid, its structure includes: culture dish body, culture fluid
Hold chamber, sealing-plug, culture fluid adding tube, culture fluid adder, culture fluid interpolation piston, training
Nutrient solution add bar, culture fluid sprinkler head, degerming ventilation ball, leakage fluid dram, bleed plug, soft iron block,
Discharge opeing electromagnetic core, power supply, circuit, solenoid, switch, power source cavity, spring, pump bowl,
Bleeding piston rod, waste liquid outlet, bottom plug, described culture fluid holds chamber and is fixed on culture dish body
Bottom, on the bottom surface of described culture dish body process leakage fluid dram, bleed plug is arranged in leakage fluid dram,
The bottom fixed installation soft iron block of bleed plug, the bottom of described bleed plug is fixed on training by spring
Nutrient solution holds on the bottom surface of intracavity, and culture fluid holds the right side bottom chamber and arranges power source cavity, described
Discharge opeing electromagnetic core is fixedly mounted on culture fluid and holds the central authorities of chamber exterior bottom, discharge opeing electromagnetic core
Installation site in the underface of bleed plug, described culture fluid adding tube is arranged in sealing-plug
Centre, culture fluid adder is arranged on the top of culture fluid adding tube, and culture fluid sprinkler head is arranged on training
The bottom of nutrient solution adding tube, described culture fluid adds piston and is arranged in culture fluid adder, training
Nutrient solution is added bar and is arranged on culture fluid interpolation piston.
Further, described pump bowl is arranged on culture fluid and holds the top on the left of chamber, piston of bleeding
Bar is arranged in pump bowl, and described solenoid is wrapped on discharge opeing electromagnetic core, solenoid
Being connected with power supply by circuit, power supply is arranged in power source cavity, and described switch series joins on the line.
Further, degerming ventilation ball is installed on the right side of described culture dish body, and described culture fluid is contained
Putting the left side processing-waste outlet bottom chamber, bottom plug is arranged in waste liquid outlet.
The beneficial effects of the utility model: this utility model uses culture fluid adder and discharge opeing electromagnetism
The design of iron core, reaches to realize the discharge of culture fluid and interpolation under conditions of being not switched on culture dish
Purpose, its design science is reasonable, and simple in construction is easy to use.
Accompanying drawing illustrates:
For ease of explanation, this utility model is embodied as and accompanying drawing is described in detail by following.
Fig. 1 is structural representation of the present utility model.
In figure: 1-culture dish body；2-culture fluid holds chamber；3-sealing-plug；4-culture fluid adding tube；
5-culture fluid adder；6-culture fluid adds piston；7-culture fluid adds bar；8-culture fluid sprays
Head；9-degerming ventilation ball；10-leakage fluid dram；11-bleed plug；12-soft iron block；13-discharge opeing electromagnetism
Iron core；14-power supply；15-circuit；16-solenoid；17-switchs；18-power source cavity；19-bullet
Spring；20-pump bowl；21-bleeds piston rod；22-waste liquid outlet；23-bottom plug.
Detailed description of the invention:
For making the purpose of this utility model, technical scheme and advantage of greater clarity, below by
Specific embodiment shown in accompanying drawing describes this utility model.However, it should be understood that these describe
The most exemplary, and it is not intended to limit scope of the present utility model.Additionally, in the following description,
Eliminate the description to known features and technology, with avoid unnecessarily obscuring of the present utility model generally
Read.
As it is shown in figure 1, the Tissue Culture Dish of a kind of replaceable culture fluid, its structure includes: cultivate
Ware body 1, culture fluid hold chamber 2, sealing-plug 3, culture fluid adding tube 4, culture fluid adder 5,
Culture fluid add piston 6, culture fluid add bar 7, culture fluid sprinkler head 8, degerming ventilation ball 9,
Leakage fluid dram 10, bleed plug 11, soft iron block 12, discharge opeing electromagnetic core 13, power supply 14, circuit 15,
Solenoid 16, switch 17, power source cavity 18, spring 19, pump bowl 20, piston rod 21 of bleeding,
Waste liquid outlet 22, bottom plug 23, described culture fluid holds chamber 2 and is fixed on the end of culture dish body 1
Portion, the bottom surface of described culture dish body 1 processes leakage fluid dram 10, and bleed plug 11 is arranged on leakage fluid dram
In 10, the bottom fixed installation soft iron block 12 of bleed plug 11, the bottom of described bleed plug 11 leads to
Crossing spring 19 to be fixed on the bottom surface that culture fluid holds in chamber 2, culture fluid holds the right side bottom chamber 2
Side arranges power source cavity 18, and described discharge opeing electromagnetic core 13 is fixedly mounted on culture fluid and holds outside chamber 2
The central authorities of side bottom, the installation site of discharge opeing electromagnetic core 13 in the underface of bleed plug 11, institute
The culture fluid adding tube 4 stated is arranged on the central authorities of sealing-plug 3, and culture fluid adder 5 is arranged on training
The top of nutrient solution adding tube 4, culture fluid sprinkler head 8 is arranged on the bottom of culture fluid adding tube 4,
Described culture fluid adds piston 6 and is arranged in culture fluid adder 5, and culture fluid adds bar 7 and pacifies
It is contained in culture fluid to add on piston 6.
Specifically, described pump bowl 20 is arranged on culture fluid and holds the top on the left of chamber 2, bleeds
Piston rod 21 is arranged in pump bowl 20, and described solenoid 16 is wrapped in discharge opeing electromagnetic core
On 13, solenoid 16 is connected with power supply 14 by circuit 15, and power supply 14 is arranged on power source cavity
In 18, described switch 17 is connected on circuit 15, and the right side of described culture dish body 1 is installed
Degerming ventilation ball 9, described culture fluid holds the left side processing-waste outlet 22 bottom chamber 2,
Bottom plug 23 is arranged in waste liquid outlet 22.
Using method of the present utility model is: is installed on culture dish body 1 by sealing-plug 3, newly trains
Nutrient solution is stored in culture fluid adder 5, when carrying out cell culture fluid and changing, first with taking out
Culture fluid is held the air in chamber 2 and extracts out by inflator 20, ON switch 17, discharge opeing electromagnetic core
13 energising after produce electromagnetic force, the soft iron block 12 in bleed plug 11 by electromagnetic attracting force to moving down
Dynamic, bleed plug 11 is opened, and the old cell culture fluid in culture dish body 1 is discharged to culture fluid and holds chamber
In 2；Promoting culture fluid to add bar 7, culture fluid adds new by culture fluid adder 5 of piston 6
Culture fluid adds in culture dish body 1 by culture fluid adding tube 4, it is achieved be not switched on culture dish
Under conditions of body 1, it is achieved culture fluid adds, thus avoid extraneous pathogen contamination cell can
Energy.Finally disconnecting switch 17, the soft iron block in bleed plug 11 moves, bleed plug 11 is closed, and beats
Driving bottom plug 23, old cell culture fluid can be discharged.
Of the present utility model ultimate principle, principal character and this utility model have more than been shown and described
Advantage.Skilled person will appreciate that of the industry, this utility model is not limited by above-described embodiment
System, simply illustrates principle of the present utility model described in above-described embodiment and description, is not taking off
On the premise of this utility model spirit and scope, this utility model also has various changes and modifications,
In the range of these changes and improvements both fall within claimed this utility model.This utility model requirement
Protection domain is defined by appending claims and equivalent thereof.
Claims (3)
1. the Tissue Culture Dish of a replaceable culture fluid, it is characterised in that: structure includes: culture dish body,
Culture fluid holds chamber, sealing-plug, culture fluid adding tube, culture fluid adder, culture fluid interpolation work
Plug, culture fluid add bar, culture fluid sprinkler head, degerming ventilation ball, leakage fluid dram, bleed plug, soft
Iron block, discharge opeing electromagnetic core, power supply, circuit, solenoid, switch, power source cavity, spring,
Pump bowl, piston rod of bleeding, waste liquid outlet, bottom plug, described culture fluid holds chamber and is fixed on
The bottom of culture dish body, the bottom surface of described culture dish body processes leakage fluid dram, and bleed plug is arranged on
In leakage fluid dram, the bottom fixed installation soft iron block of bleed plug, bullet is passed through in the bottom of described bleed plug
Spring is fixed on culture fluid and holds on the bottom surface of intracavity, and the right side that culture fluid holds bottom chamber arranges power supply
Chamber, described discharge opeing electromagnetic core is fixedly mounted on culture fluid and holds the central authorities of chamber exterior bottom, institute
The culture fluid adding tube stated is arranged on the central authorities of sealing-plug, and culture fluid adder is arranged on culture fluid and adds
Adding the top of pipe, culture fluid sprinkler head is arranged on the bottom of culture fluid adding tube, described culture fluid
Adding piston to be arranged in culture fluid adder, culture fluid adds bar and is arranged on culture fluid interpolation piston
On.
The Tissue Culture Dish of a kind of replaceable culture fluid the most according to claim 1, it is characterised in that:
Described pump bowl is arranged on culture fluid and holds the top on the left of chamber, and piston rod of bleeding is arranged on bleeds
In cylinder, described solenoid is wrapped on discharge opeing electromagnetic core, and solenoid is by circuit and electricity
Source connects, and power supply is arranged in power source cavity, and described switch series joins on the line.
The Tissue Culture Dish of a kind of replaceable culture fluid the most according to claim 1, it is characterised in that:
Degerming ventilation ball is installed on the right side of described culture dish body, and described culture fluid holds the left side bottom chamber
Side processing-waste outlet, bottom plug is arranged in waste liquid outlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620349167.3U CN205741061U (en) | 2016-04-25 | 2016-04-25 | A kind of Tissue Culture Dish of replaceable culture fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620349167.3U CN205741061U (en) | 2016-04-25 | 2016-04-25 | A kind of Tissue Culture Dish of replaceable culture fluid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205741061U true CN205741061U (en) | 2016-11-30 |
Family
ID=57371577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620349167.3U Expired - Fee Related CN205741061U (en) | 2016-04-25 | 2016-04-25 | A kind of Tissue Culture Dish of replaceable culture fluid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205741061U (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520550A (en) * | 2016-12-30 | 2017-03-22 | 北京工业大学 | Micro-channel PCR amplification system based on electromagnetic propulsion |
| CN108179109A (en) * | 2018-03-12 | 2018-06-19 | 青岛农业大学 | A kind of microorganism intelligence liquid-based culture dish |
| CN111040948A (en) * | 2020-01-13 | 2020-04-21 | 中国人民解放军第四军医大学 | Liquid changing device for multi-cell co-culture simulated weightlessness experiment |
| CN112522090A (en) * | 2020-12-04 | 2021-03-19 | 潘国红 | Automatic culture solution injection device for biological culture dish |
| WO2021114232A1 (en) * | 2019-12-12 | 2021-06-17 | 生合生物科技（扬州）有限公司 | Automatically controlled strain culture dish |
| CN114456934A (en) * | 2022-03-02 | 2022-05-10 | 邹玉良 | Intelligent automatic culture apparatus for tissue culture |
| CN117343837A (en) * | 2023-10-07 | 2024-01-05 | 中国人民解放军总医院第六医学中心 | Stem cell continuous liquid-changing culture device and application method thereof |
-
2016
- 2016-04-25 CN CN201620349167.3U patent/CN205741061U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520550A (en) * | 2016-12-30 | 2017-03-22 | 北京工业大学 | Micro-channel PCR amplification system based on electromagnetic propulsion |
| CN106520550B (en) * | 2016-12-30 | 2019-04-26 | 北京工业大学 | A kind of fluid channel PCR amplification system based on electromagnetic propulsion |
| CN108179109A (en) * | 2018-03-12 | 2018-06-19 | 青岛农业大学 | A kind of microorganism intelligence liquid-based culture dish |
| WO2021114232A1 (en) * | 2019-12-12 | 2021-06-17 | 生合生物科技（扬州）有限公司 | Automatically controlled strain culture dish |
| CN111040948A (en) * | 2020-01-13 | 2020-04-21 | 中国人民解放军第四军医大学 | Liquid changing device for multi-cell co-culture simulated weightlessness experiment |
| CN112522090A (en) * | 2020-12-04 | 2021-03-19 | 潘国红 | Automatic culture solution injection device for biological culture dish |
| CN112522090B (en) * | 2020-12-04 | 2021-07-02 | 潘国红 | Automatic culture solution injection device for biological culture dish |
| CN114456934A (en) * | 2022-03-02 | 2022-05-10 | 邹玉良 | Intelligent automatic culture apparatus for tissue culture |
| CN114456934B (en) * | 2022-03-02 | 2024-01-26 | 上海碧博生物医药工程有限公司 | Intelligent automatic culture device for tissue culture |
| CN117343837A (en) * | 2023-10-07 | 2024-01-05 | 中国人民解放军总医院第六医学中心 | Stem cell continuous liquid-changing culture device and application method thereof |
| CN117343837B (en) * | 2023-10-07 | 2024-04-05 | 中国人民解放军总医院第六医学中心 | Stem cell continuous liquid-changing culture device and application method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20161117 Address after: 261053 Shandong Province, Weifang city Weicheng District Baotong Street No. 7166 Weifang Medical University Patentee after: Weifang Medical University Address before: 261053 Shandong Province, Weifang city Weicheng District Baotong Street No. 7166 Patentee before: Gao Xianghong |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161130 Termination date: 20170425 |