CN114326847A - Intelligent pH value regulating and controlling device and method for protein modification research - Google Patents
Intelligent pH value regulating and controlling device and method for protein modification research Download PDFInfo
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Abstract
The invention relates to the technical field of protein modification, in particular to intelligent PpH value regulating and controlling equipment for protein modification research, which comprises a base, two telescopic rods, a rotary disk, a transverse pipe, two bent pipes, two containing cups, an acid box, an alkali box, two regulators, a timer and a controller, wherein the rotary disk is arranged on the base, the two telescopic rods are symmetrically arranged on the rotary disk, the two telescopic rods are rotatably connected with the middle part of the transverse pipe, a settling part is arranged in the middle of the transverse pipe, a discharge opening is formed in the settling part, the two containing cups are respectively connected with the two ends of the transverse pipe through the two bent pipes, bent pipe electromagnetic valves are arranged at the bent pipes, the regulators are respectively arranged at the positions, close to the containing cups, of the transverse pipe, the acid box and the alkali box are both connected with the regulators, the regulators detect and regulate PpH values of liquid in the transverse pipe, and the bent pipe electromagnetic valves, the regulators, the timers, the telescopic rods and the rotary disk are all connected with the controller. The substantial effects of the invention are as follows: by locally varying the PpH value of the liquid in the cross tube, a better PpH value shift effect is provided.
Description
Technical Field
The invention relates to the technical field of protein modification, in particular to intelligent PH value regulation and control equipment and method for protein modification research.
Background
The pH shift technique is a novel technique for modifying a protein, which can change the properties of the protein. By modifying and researching proteins, the development and utilization of the value of the proteins can be expanded. For example, Chinese patent CN111053145A, published as 2020, 4, 24, discloses a method for improving solubility of potato protein by ultrasonic and pH shift, belonging to the technical field of protein modification. Which comprises the following steps: (1) peeling potato, cutting into pieces, and soaking in 0.12% sodium sulfite solution; (2) standing the potato homogenate for 15min, centrifuging to obtain a supernatant, adjusting the pH to 8.0, stirring and leaching at room temperature for 2h, centrifuging to obtain a supernatant, adjusting the pH to 4.0, standing for 1h, re-dissolving the precipitate with distilled water, adjusting the pH to 7.0, and freeze-drying to obtain potato powder; (3) preparing the obtained potato protein into proper concentration for ultrasonic treatment; (4) and (4) adjusting the pH of the protein solution subjected to ultrasonic treatment in the step (3) to 12.0, maintaining for 1h, and adjusting the pH back to 7.0 to obtain a modified potato protein solution. The modification method provided by the method can greatly improve the solubility of the potato protein, and has very important significance for expanding the application field of the potato protein. Although the technical proposal provides that the potato protein is alternately placed in the acidic environment and the alkaline environment, the dissolution character of the potato protein can be changed. However, the PH shift techniques for altering the flax protein profile are not identical. Therefore, research on equipment for conveniently researching the modification of flax protein is needed. However, the current research on the pH value modification of the protein lacks special equipment, and the research on the pH value modification is inefficient. Therefore, development of a modification research device capable of increasing the pH value of the protein is required.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the technical problem of the lack of protein pH value modification research equipment at present. Provides an intelligent pH value regulating and controlling device and method for protein modification research.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: the utility model provides an intelligent regulation and control pH value equipment for modified research of protein, includes base, two telescopic links, rotary disk, violently manages, two return bends, two appearance cups, acid tank, alkali case, two regulators, time-recorder and controller, the rotary disk is installed on the base, two the telescopic link symmetry is installed on the rotary disk, two the telescopic link rotates with violently managing the middle part and is connected, violently manages the centre and is equipped with the portion of subsiding, the discharge opening has been seted up to the portion of subsiding, two appearance cups are connected at violently managing both ends through two return bends respectively, and elbow department is equipped with the return bend solenoid valve, and two regulators are installed respectively and are close to violently managing two appearance cup departments, and acid tank and alkali case all are connected with the regulator, the regulator detects and adjusts the pH value of violently managing interior liquid, and return bend solenoid valve, regulator, time-recorder, telescopic link and rotary disk all are connected with the controller.
Preferably, the containing cup is provided with a vent valve and a liquid filling opening.
Preferably, the regulator comprises an adjusting sleeve, two pH value sensors and two adjusting cylinders, the adjusting sleeve is sleeved on the horizontal pipe, the horizontal pipe is hollowed corresponding to the position of the adjusting sleeve, the two pH value sensors are respectively installed at two ends of the adjusting sleeve, the two adjusting cylinders are installed on the adjusting sleeve, the front end of each adjusting cylinder is communicated with the transverse rod, each adjusting cylinder comprises a cylinder body, a liquid adding port, a driving spring, an electronic switch, a sliding block, a one-way valve clack, an injection pipe and a connecting pipe, the injection pipe and the connecting pipe are both installed at the front end of the cylinder body, the liquid adding port is communicated with the horizontal pipe, the connecting pipes of the two adjusting pipes are respectively communicated with an acid box and an alkali box, the one-way valve clack is respectively arranged in the injection pipe and the connecting pipe, the sliding block is installed in the cylinder body, the outer wall of the sliding block is abutted against the inner wall of the cylinder body, and the driving spring is installed in the cylinder body, the driving spring is positioned between the sliding block and the front end of the barrel, two ends of the driving spring are fixedly connected with the front end of the barrel and the sliding block respectively, two ends of the driving spring are connected with the electronic switch and the direct-current power supply in series through wires, and the pH value sensor and the electronic switch are connected with the controller.
Preferably, the liquid adding port extends into the transverse pipe, a notch is formed in the front end of the liquid adding port, a plurality of hollow-out ports are formed in the front portion of the liquid adding port, and the extending directions of the notch and the hollow-out ports are perpendicular to the flowing direction of liquid in the transverse pipe.
Preferably, the cup wall of the cup is provided with two sections which are vertical to each other, and when the transverse pipe is in a horizontal state, the bottom of the front section of the cup wall is horizontal.
A method for intelligently controlling pH for protein modification studies, using an intelligent pH control device for protein modification studies as described above, comprising: injecting the liquid containing protein into any one of the cups; inputting the target pH value into a controller, controlling the telescopic rod to act by the controller, enabling the containing cup containing the liquid to rise, opening the electromagnetic valve of the bent pipe, and enabling the liquid to flow into the other containing cup through the bent pipe; the pH value sensor of the regulator with a higher position periodically detects the pH value of the liquid, the controller calculates to obtain the amount of acid agent or alkali agent required by regulating to the target pH value, and the acid agent or alkali agent is injected into the transverse pipe through the regulator with the higher position; and a pH value sensor of the regulator with a lower position periodically detects the pH value of the liquid, the controller calculates to obtain the acid regulating agent amount or alkali regulating agent amount required by regulating to the target pH value, and the acid regulating agent amount or alkali regulating agent amount is injected into the transverse pipe through the regulator with the lower position.
Preferably, the centrifugal rotation speed and the centrifugal duration are input into a controller, the controller controls the telescopic rod to enable the transverse pipe to be horizontal, and the two bent pipe electromagnetic valves are opened; controlling the rotating disc to rotate according to the target rotating speed and the target duration; after the rotating disc finishes rotating, opening the discharge port, and taking the container to contain the liquid discharged from the discharge port; if the supernatant is needed, the containing cup and the transverse pipe are cleaned, then the liquid discharged from the discharge opening is poured into the containing cup, if the sediment is taken out, the discharge opening is closed, and the liquid discharged from the discharge opening is discarded.
The substantial effects of the invention are as follows: the pH value of the liquid can be changed more uniformly and more rapidly by locally changing the pH value of the liquid in the transverse pipe, and a better pH value deviation effect can be provided; the pH value can be controlled more accurately by adjusting through two regulators and respectively performing rough adjustment and fine adjustment; can directly carry out centrifugal operation in a transverse pipe and a containing cup, and improve the efficiency of the research on the modification of the pH value of the protein.
Drawings
FIG. 1 is a schematic diagram of an apparatus for intelligently controlling pH according to an embodiment.
FIG. 2 is a schematic diagram of an apparatus for intelligently controlling pH according to an embodiment.
FIG. 3 is a schematic diagram of an embodiment of a regulator.
FIG. 4 is a schematic view of a plating port according to an embodiment of the present invention.
Wherein: 11. the device comprises a liquid filling opening, 12, a vent valve, 13, a containing cup, 21, a regulator, 22, an acid tank, 23, an alkali tank, 31, a transverse pipe, 32, a settling part, 33, a discharge opening, 41, a base, 42, a rotating disk, 43, a telescopic rod, 501, an adjusting cylinder, 502, a sliding block, 503, a pH value sensor, 504, an adjusting sleeve, 505, a driving spring, 506, a connecting pipe, 507, a liquid adding opening, 601, a hollow opening, 602 and a notch.
Detailed Description
The following provides a more detailed description of the present invention, with reference to the accompanying drawings.
The first embodiment is as follows:
an intelligent pH value regulation and control device for protein modification research, please refer to figure 1, which comprises a base 41, two telescopic rods 43, a rotating disk 42, a transverse pipe 31, two bent pipes, two containing cups 13, an acid tank 22, an alkali tank 23, two regulators 21, a timer and a controller, wherein the rotating disk 42 is installed on the base 41, the two telescopic rods 43 are symmetrically installed on the rotating disk 42, the two telescopic rods 43 are rotatably connected with the middle part of the transverse pipe 31, a settling part 32 is arranged in the middle of the transverse pipe 31, the settling part 32 is provided with a discharge opening 33, the two containing cups 13 are respectively connected with the two ends of the transverse pipe 31 through the two bent pipes, bent pipe electromagnetic valves are arranged at the bent pipes, the two regulators 21 are respectively installed at the positions, close to the two containing cups 13, of the transverse pipe 31, the acid tank 22 and the alkali tank 23 are both connected with the regulators 21, the regulators 21 detect and regulate the pH value of liquid in the transverse pipe 31, the bent pipe electromagnetic valves, the regulators 21, The timer, the telescopic rod 43 and the rotating disk 42 are all connected with the controller. The cup 13 is provided with a breather valve 12 and a filling opening 11.
Referring to fig. 2, for the operation of the apparatus for intelligently regulating and controlling pH, the horizontal tube 31 is tilted by the telescopic rod 43, so that the liquid in the left cup 13 flows to the right cup 13 through the horizontal tube 31. When the liquid is contained in the cup 13 on the right side, the horizontal tube 31 may be inclined to the other side by the expansion link 43. When the liquid flows through the horizontal pipe 31, the regulator 21 detects the pH of the liquid, and calculates the amount of acid or alkali required to adjust the pH to a target value. The acid agent used in this example was HCl solution and the alkaline agent was NaOH solution.
Referring to fig. 3, the adjuster 21 includes an adjusting sleeve 504, two pH sensors 503 and two adjusting cylinders 501, the adjusting sleeve 504 is sleeved on the horizontal pipe 31, the horizontal pipe 31 is hollow corresponding to the adjusting sleeve 504, the two pH sensors 503 are respectively installed at two ends of the adjusting sleeve 504, the two adjusting cylinders 501 are installed on the adjusting sleeve 504, the front end of the adjusting cylinder 501 is communicated with the cross bar, the adjusting cylinder 501 includes a cylinder body, a liquid adding port 507, a driving spring 505, an electronic switch, a slider 502, a one-way valve flap, an injection pipe and a connecting pipe 506, the injection pipe and the connecting pipe 506 are both installed at the front end of the cylinder body, the liquid adding port 507 is communicated with the horizontal pipe 31, the connecting pipes 506 of the two adjusting pipes are respectively communicated with an acid tank 22 and an alkali tank 23, the injection pipe and the connecting pipe 506 are both provided with the one-way valve flap, the slider 502 is installed in the cylinder body, the outer wall of the slider 502 is abutted against the inner wall of the cylinder body, the driving spring 505 is installed in the cylinder body, the driving spring 505 is positioned between the slide block 502 and the front end of the cylinder body, two ends of the driving spring 505 are respectively and fixedly connected with the front end of the cylinder body and the slide block 502, two ends of the driving spring 505 are connected with the electronic switch and the direct-current power supply in series through conducting wires, and the pH value sensor 503 and the electronic switch are connected with the controller.
By controlling the on duty of the electronic switch, the amount of contraction of the drive spring 505, i.e., the position of the slider 502, can be controlled. When the slide block 502 retreats, the acid agent or the alkali agent in the acid tank 22 or the alkali tank 23 flows into the cylinder, and when the slide block 502 advances, the acid agent or the alkali agent in the cylinder is added into the horizontal tube 31, so that the pH value of the liquid in the horizontal tube 31 is changed. The pH value of the liquid in the transverse pipe 31 is detected by the pH value sensor 503, the volume of the liquid can be calculated by combining the cross section area, the detection period and the liquid flow rate of the transverse pipe 31, and the amount of the acid agent or the alkali agent to be added can be calculated according to the volume and the pH value of the liquid. The higher-positioned regulator 21 performs coarse adjustment and the lower-positioned regulator 21 performs fine adjustment.
Referring to fig. 4, the liquid adding port 507 extends into the horizontal tube 31, a notch 602 is formed at the front end of the liquid adding port 507, a plurality of hollow-out ports 601 are formed at the front portion of the liquid adding port 507, and the extending directions of the notch 602 and the hollow-out ports 601 are perpendicular to the flowing direction of the liquid in the horizontal tube 31. The cup wall of the cup container 13 has two sections perpendicular to each other, and when the transverse tube 31 is in a horizontal state, the bottom of the front section of the cup wall is horizontal.
A method for intelligently controlling pH for protein modification studies, using an intelligent pH control device for protein modification studies as described above, comprising: injecting the liquid containing the protein into any one of the cups 13; inputting the target pH value into a controller, controlling the telescopic rod 43 to act by the controller, enabling the containing cup 13 containing the liquid to rise, opening the electromagnetic valve of the elbow pipe, and enabling the liquid to flow into the other containing cup 13 through the elbow pipe; the pH value sensor 503 of the regulator 21 with a higher position periodically detects the pH value of the liquid, the controller calculates to obtain the amount of acid agent or alkali agent required by regulating to the target pH value, and the acid agent or alkali agent is injected into the transverse pipe 31 through the regulator 21 with a higher position; the pH sensor 503 of the lower regulator 21 periodically detects the pH of the liquid, and the controller calculates the amount of the acid or alkali to be adjusted to the target pH, and injects the acid or alkali into the horizontal pipe 31 through the lower regulator 21.
Inputting the centrifugal rotation speed and the centrifugal duration into a controller, controlling the telescopic rod 43 by the controller to enable the transverse pipe 31 to be horizontal, and opening the two bent pipe electromagnetic valves; controlling the rotating disc 42 to rotate according to the target rotating speed and the target duration; after the rotation of the rotary disk 42 is finished, the discharge opening 33 is opened, and the container is taken to contain the liquid discharged from the discharge opening 33; if the supernatant is required, the vessel 13 and the horizontal pipe 31 are cleaned, and then the liquid discharged from the discharge port 33 is poured into the vessel 13, and if the precipitate is to be taken out, the discharge port 33 is closed, and the liquid discharged from the discharge port 33 is discarded. For centrifugation, the acid tank 22 and the alkali tank 23 should be disposed on the rotation axis of the rotating disk 42.
The beneficial technical effects of this embodiment are: by locally changing the pH value of the liquid in the transverse pipe 31, the pH value of the liquid can be changed more uniformly and more rapidly, and a better pH value shift effect can be provided; the pH value can be controlled more accurately by adjusting through two regulators 21 and performing rough adjustment and fine adjustment respectively; the centrifugal operation can be directly carried out in the transverse pipe 31 and the containing cup 13, and the efficiency of the research on the modification of the pH value of the protein is improved.
The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.
Claims (7)
1. An intelligent pH value regulating and controlling device for protein modification research, which is characterized in that,
including base, two telescopic links, rotary disk, violently manage, two return bends, two appearance cups, acid tank, alkali case, two regulators, time-recorder and controller, the rotary disk is installed on the base, two the telescopic link symmetry is installed on the rotary disk, two the telescopic link with violently manage the middle part and rotate and be connected, violently manage the centre and be equipped with the portion of subsiding, the discharge opening has been seted up to the portion of subsiding, two appearance cups connect respectively through two return bends and violently manage both ends, and return bend department is equipped with the return bend solenoid valve, and two regulators are installed respectively and are violently managed near two appearance cup departments, and acid tank and alkali case all are connected with the regulator, the regulator detects and adjusts the pH value of violently managing interior liquid, and return bend solenoid valve, regulator, time-recorder, telescopic link and rotary disk all are connected with the controller.
2. The intelligent pH value control device for protein modification research according to claim 1,
the containing cup is provided with a vent valve and a liquid feeding port.
3. The intelligent pH value control device for protein modification research according to claim 1 or 2,
the regulator comprises an adjusting sleeve, two pH value sensors and two adjusting barrels, the adjusting sleeve is sleeved on the transverse pipe, the transverse pipe is hollowed corresponding to the position of the adjusting sleeve, the two pH value sensors are respectively installed at two ends of the adjusting sleeve, the two adjusting barrels are installed on the adjusting sleeve, the front end of the adjusting barrel is communicated with the transverse rod, the adjusting barrel comprises a barrel body, a liquid adding port, a driving spring, an electronic switch, a sliding block, a one-way valve clack, an injection pipe and a connecting pipe, the injection pipe and the connecting pipe are both installed at the front end of the barrel body, the liquid adding port is communicated with the transverse pipe, the connecting pipes of the two adjusting pipes are respectively communicated with an acid box and an alkali box, the one-way valve clack is respectively arranged in the injection pipe and the connecting pipe, the sliding block is installed in the barrel body, the outer wall of the sliding block is abutted against the inner wall of the barrel body, the driving spring is installed in the barrel body, the driving spring is positioned between the sliding block and the front end of the barrel body, two ends of the driving spring are respectively and fixedly connected with the front end of the cylinder body and the sliding block, two ends of the driving spring are connected with the electronic switch and the direct-current power supply in series through leads, and the pH value sensor and the electronic switch are connected with the controller.
4. The intelligent pH value control device for protein modification research according to claim 3,
the liquid adding port extends into the transverse pipe, a notch is formed in the front end of the liquid adding port, a plurality of hollow ports are formed in the front portion of the liquid adding port, and the extending directions of the notch and the hollow ports are perpendicular to the flowing direction of liquid in the transverse pipe.
5. The intelligent pH value control device for protein modification research according to claim 1 or 2,
the cup wall of the cup is provided with two sections which are vertical to each other, and when the transverse pipe is in a horizontal state, the bottom of the front section of the cup wall is horizontal.
6. A method for intelligently controlling pH value for protein modification research, which uses the intelligent pH value control device for protein modification research according to any one of claims 1 to 5,
the method comprises the following steps:
injecting the liquid containing protein into any one of the cups;
inputting the target pH value into a controller, controlling the telescopic rod to act by the controller, enabling the containing cup containing the liquid to rise, opening the electromagnetic valve of the bent pipe, and enabling the liquid to flow into the other containing cup through the bent pipe;
the pH value sensor of the regulator with a higher position periodically detects the pH value of the liquid, the controller calculates to obtain the amount of acid agent or alkali agent required by regulating to the target pH value, and the acid agent or alkali agent is injected into the transverse pipe through the regulator with the higher position;
and a pH value sensor of the regulator with a lower position periodically detects the pH value of the liquid, the controller calculates to obtain the acid regulating amount or the alkali regulating amount required by regulating to the target pH value, and the acid regulating amount or the alkali regulating amount is injected into the transverse pipe through the regulator with the lower position.
7. The method of claim 6, wherein the pH value is adjusted according to the protein modification,
inputting the centrifugal rotation speed and the centrifugal duration into a controller, controlling the telescopic rod by the controller to enable the transverse pipe to be horizontal, and opening the two bent pipe electromagnetic valves;
controlling the rotating disc to rotate according to the target rotating speed and the target duration;
after the rotating disc finishes rotating, opening the discharge port, and taking the container to contain the liquid discharged from the discharge port;
if the supernatant is needed, the containing cup and the transverse pipe are cleaned, then the liquid discharged from the discharge opening is poured into the containing cup, if the sediment is taken out, the discharge opening is closed, and the liquid discharged from the discharge opening is discarded.
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Cited By (2)
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| CN114262357A (en) * | 2021-12-29 | 2022-04-01 | 浙江旅游职业学院 | Flax protein modification equipment and method based on PpH migration technology |
| CN114651893A (en) * | 2022-04-21 | 2022-06-24 | 大连非得生物产业有限公司 | Sea cucumber peptide deep-processed food and preparation method thereof |
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| CN114262357B (en) * | 2021-12-29 | 2023-08-11 | 浙江旅游职业学院 | Flax protein modification equipment and method based on PH offset technology |
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