CN114276407B - Device and method for extracting protein from bone or shell by pH shift technology - Google Patents
Device and method for extracting protein from bone or shell by pH shift technology Download PDFInfo
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- CN114276407B CN114276407B CN202111628091.XA CN202111628091A CN114276407B CN 114276407 B CN114276407 B CN 114276407B CN 202111628091 A CN202111628091 A CN 202111628091A CN 114276407 B CN114276407 B CN 114276407B
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Abstract
The invention relates to the technical field of protein processing equipment, in particular to equipment and a method for extracting protein from bones or shells by adopting a PH offset technology. The invention has the following substantial effects: can change the structure of the protein more effectively, increase the solubility of the protein and facilitate the extraction of the protein.
Description
The invention relates to the technical field of protein processing equipment, in particular to equipment and a method for extracting protein from bones or shells by adopting a PH offset technology.
Background
The shell is mainly composed of an inorganic phase and an organic phase, wherein the inorganic phase is about 95-99.9% CaCO3. The organic phase is composed of about 0.1 to 5% of organic matter, proteins, glycoproteins, polysaccharides, chitin, lipids, etc., and can be mainly divided into an acid-soluble component, an acid-insoluble-denaturant-soluble component, and an acid-insoluble-denaturant-insoluble component. It is found that signal molecules capable of promoting cell osteogenic differentiation exist in the shell organic matrix, and the signal factors can activate the activity of cell alkaline phosphatase, promote the expression of certain specific proteins and genes in the cell osteogenic differentiation process, induce cell body mineralization and the like, so that the shell part structure shows good biocompatibility in an in-vivo environment. The research shows that the human bone marrow stromal cells grow and secrete cell stroma on the pearl layer human bone material, the pearl layer-polylactic acid composite artificial bone material has no obvious influence on the proliferation of bone marrow stromal cells, and shows good biocompatibility. Therefore, based on the biocompatibility of the shell, the method can be used for researching shell-based biological materials. Thus, the method has important significance for researching the proteins of the shells and bones. But currently there is a lack of efficient techniques for extracting proteins from bones or shells. The PH shift technique is a recently developed protein modification technique that can alter various properties of proteins, including solubility. Thus, there is a need to study techniques for extracting proteins based on PH shift techniques.
For example, chinese patent CN111053145A, publication date 2020, month 4 and 24, a method for improving potato protein solubility by combining ultrasonic and pH shift, belongs to the technical field of protein modification. The method comprises the following steps: (1) Peeling and cutting potato into pieces, and immersing the pieces in 0.12% sodium sulfite solution; (2) Homogenizing potato, standing for 15min, centrifuging to obtain supernatant, adjusting pH to 8.0, stirring at room temperature, leaching for 2 hr, centrifuging to obtain supernatant, adjusting pH to 4.0, standing for 1 hr, adding distilled water into precipitate, redissolving, adjusting pH to 7.0, and lyophilizing to obtain potato powder; (3) Preparing the obtained potato protein into proper concentration for ultrasonic treatment; (4) And (3) regulating the pH of the protein solution subjected to ultrasonic treatment in the step (3) to 12.0, maintaining for 1h, and regulating the pH back to 7.0 to obtain the modified potato protein solution. The modification method provided by the technical scheme can greatly improve the solubility of the potato protein, and has very important significance for expanding the application field of the potato protein. However, the technical solution is not suitable for improving the solubility of the protein in the bone and the shell.
Disclosure of Invention
The invention aims to solve the technical problems that: there is currently a lack of technical problem with schemes for efficient extraction of proteins from bone or shell. An apparatus and method for extracting proteins from bone or shell using PH shift technology is provided that can be used to study what PH shift is most effective in extracting proteins from bone or shell.
In order to solve the technical problems, the invention adopts the following technical scheme: the utility model provides an adopt PH skew technique to draw equipment of protein in follow bone or shell, includes base, two telescopic links, violently pipe, two return bend, two hold cup, two solenoid valves, acid regulator, alkali regulator, acid case, alkali case, time-recorder and controller, two hold the cup and connect at violently pipe both ends through two return bend respectively, two solenoid valves are installed respectively on two return bend, two the telescopic link rotates with violently pipe middle part to be connected, and the telescopic link is installed on the base, is equipped with the subsider in the middle of the violently pipe, the subsider bottom is equipped with the fluid-discharge tube, install the electrically controlled valve on the fluid-discharge tube, acid regulator and alkali regulator are installed respectively in violently pipe both sides, acid case and alkali case are connected with acid regulator and alkali regulator respectively, acid regulator and alkali regulator homoenergetic detect and adjust the PH value of violently intraductal liquid, time-recorder, solenoid valve, electrically controlled valve, telescopic link, acid regulator and alkali regulator all are connected with the controller.
Preferably, the containing cup is provided with a ventilation valve and a liquid filling port.
Preferably, the bent pipe is divided into two parts, and the two parts are detachably connected through a sealing interface.
Preferably, the acid regulator and the alkali regulator comprise regulating sleeves, two PH value sensors and regulating cylinders, the regulating sleeves are sleeved on the transverse pipes, the transverse pipes are hollow corresponding to the positions of the regulating sleeves, the two PH value sensors are respectively installed at two ends of the regulating sleeves, the regulating cylinders are installed on the regulating sleeves, the front ends of the regulating cylinders are communicated with the transverse pipes, the regulating cylinders comprise a cylinder body, a driving spring, an electronic switch, a sliding block, a one-way valve clack, an injection pipe and a connecting pipe, the injection pipe and the connecting pipe are all installed at the front ends of the cylinder body, the injection pipe is communicated with the transverse pipes, the connecting pipe of the two regulating pipes is communicated with an acid tank or an alkali tank, unidirectional valve clack is arranged in the injection pipe and the connecting pipe, the sliding block is installed in the cylinder body, the outer wall of the sliding block is in butt joint with the inner wall of the cylinder body, the driving spring is installed in the cylinder body, the driving spring is located between the sliding block and the front ends of the cylinder body, the two ends of the driving spring are respectively fixedly connected with the front ends of the cylinder body, the driving spring is connected with the electronic switch and the direct-current power supply through wires, and the PH value sensors are connected with the electronic switch and the controller in series.
A method of extracting protein from bone or shell using PH shift technology, using an apparatus for extracting protein from bone or shell using PH shift technology as described above, comprising: breaking bones or shells into small blocks, disassembling a bent pipe at one side, loading the small blocks of bones or shells into a settlement part of a transverse pipe, installing the bent pipe, and checking and sealing; adding an acid solution with a preset pH value into one side containing cup, and adding an alkali solution with a preset pH value into the other side containing cup; setting a PH value time sequence, inputting the PH value time sequence into a controller, and starting a timer after the controller waits for a short time; the controller controls the telescopic rod, the electromagnetic valve, the acid regulator and the alkali regulator to act, so that the PH value of the liquid in the transverse tube changes according to the PH value time sequence; after the PH value time sequence is completed, the telescopic rod is controlled to act so as to enable the horizontal pipe to be horizontal, and the two electromagnetic valves are sequentially opened so as to enable the solution in the two containing cups to sequentially flow out of the liquid discharge pipe; collecting liquid flowing out from a liquid discharge pipe by using the same container, continuously adjusting the pH value to 7 after mixing acid and alkali, and drying to obtain the solution containing dissolved proteins; the one-sided elbow is removed, the small piece of bone or shell is removed, and protein extraction from the bone or shell is continued using conventional methods.
Preferably, the method for changing the pH value of the liquid in the transverse tube according to the pH value time sequence comprises the following steps: the controller controls the telescopic rod to act, so that the cup at one side is lifted, the electromagnetic valve is opened, and the acid solution or the alkali solution corresponding to the PH value time sequence flows into the transverse pipe and then flows into the transverse pipe; the pH value of the liquid can be measured and obtained by the acid regulator and the alkali regulator, the controller calculates the acid dosage or the alkali dosage required by regulating the target pH value, and the acid dosage or the alkali dosage is injected into the transverse tube by the corresponding acid regulator or the alkali regulator; the solution finally flows into the transverse tube from the cup with higher position, covers the sedimentation part of the transverse tube and closes the electromagnetic valve; controlling the telescopic rod to enable the horizontal pipe to be horizontal, and standing until the PH value time sequence reaches the moment of changing the PH value; if the acid-base property is not changed, the controller calculates the acid dosage or the alkali dosage required by regulating the target PH value, and the acid dosage or the alkali dosage is injected into the transverse tube through the corresponding acid regulator or the alkali regulator; if the acid-base property is changed, the controller controls the telescopic rod to act, so that the cup without solution is lowered, the electromagnetic valve with a lower position is opened, and the electromagnetic valve is closed after all the solution in the transverse tube flows into the cup; opening the electromagnetic valve at the bent pipe with higher position, and enabling the solution in the cup with higher position to flow into the transverse pipe, so that the acid-base property in the transverse pipe is rapidly changed; the pH value of the liquid can be measured and obtained by the acid regulator and the alkali regulator, the controller calculates the acid dosage or the alkali dosage required for obtaining the target pH value, and the acid dosage or the alkali dosage is injected into the transverse tube by the corresponding acid regulator or the alkali regulator.
The invention has the following substantial effects: the acid solution can be quickly replaced by the alkali solution, so that the protein is placed under the pH value deviation with high pH value change rate, the structure of the protein can be more effectively changed, the solubility of the protein is increased, and the extraction is convenient; when the acid and alkali of the solution are replaced, the acid solution or the alkali solution is firstly extracted, and the neutralization of the acid and the alkali is not needed, so that the efficiency of pH value change is improved, the research of pH value deviation on protein property change is facilitated, and the acid-alkali solution can be saved; the pH value of the solution in the transverse tube can be conveniently and accurately adjusted through the improved acid regulator and the alkali regulator.
Drawings
FIG. 1 is a schematic diagram showing the structure of an apparatus for extracting protein according to an embodiment.
FIG. 2 is a schematic diagram showing the operation of the protein extraction apparatus according to the first embodiment.
FIG. 3 is a schematic view of a method for extracting proteins from bones or shells
FIG. 4 is a schematic diagram of a method for adjusting pH in a cross-pipe according to an embodiment.
FIG. 5 is a schematic diagram of an exemplary diacid regulator.
Wherein: 101. the device comprises a ventilation valve, 102, a liquid filling port, 103, a containing cup, 201, a sealing interface, 202, an electromagnetic valve, 203, an acid regulator, 204, an acid tank, 205, an alkali tank, 206, an alkali regulator, 207, a liquid discharge pipe, 301, a material, 401, a telescopic rod, 402, a base, 501, an adjusting cylinder, 502, a sliding block, 503, a PH value sensor, 504, an adjusting sleeve, 505, a driving spring, 506 and a connecting pipe.
Detailed Description
The following description of the embodiments of the present invention will be made with reference to the accompanying drawings.
Embodiment one:
referring to fig. 1 and 2, an apparatus for extracting protein from bones or shells by PH offset technology includes a base 402, two telescopic rods 401, a horizontal tube, two bent tubes, two cups 103, two solenoid valves 202, an acid regulator 203, an alkali regulator 206, an acid tank 204, an alkali tank 205, a timer and a controller, wherein the two cups 103 are respectively connected to two ends of the horizontal tube through the two bent tubes, the two solenoid valves 202 are respectively mounted on the two bent tubes, the two telescopic rods 401 are rotatably connected with the middle of the horizontal tube, the telescopic rods 401 are mounted on the base 402, a sedimentation part is arranged in the middle of the horizontal tube, a drain 207 is arranged at the bottom of the sedimentation part, an electric control valve is mounted on the drain 207, the acid regulator 203 and the alkali regulator 206 are respectively mounted on two sides of the horizontal tube, the acid tank 204 and the alkali tank 205 are respectively connected with the acid regulator 203 and the alkali regulator 206, and the acid regulator 203 and the alkali regulator 206 can both detect and regulate PH of liquid in the horizontal tube, and the timer, the solenoid valves 202, the electric control valve, the telescopic rods 401, the acid regulator 203 and the alkali regulator 206 are connected with the controller. The containing cup 103 is provided with a ventilation valve 101 and a liquid filling port 102. The bent pipe is divided into two parts, and the two parts are detachably connected through a sealing interface 201. The liquid adding port 102 can be opened and closed, and is opened during liquid adding, and the liquid adding port 102 is closed after liquid adding is completed.
A method for extracting protein from bone or shell using PH shift technology, using an apparatus for extracting protein from bone or shell using PH shift technology as described above, referring to fig. 3, comprising: breaking bones or shells into small blocks, disassembling the bent pipe at one side, loading the small blocks of bones or shells into the sedimentation part of the transverse pipe, namely loading the material 301 into the sedimentation part of the transverse pipe, installing the bent pipe, and checking and sealing; adding an acid solution with a preset pH value into one side of the containing cup 103, and adding an alkali solution with a preset pH value into the other side of the containing cup 103; setting a PH value time sequence, inputting the PH value time sequence into a controller, and starting a timer after the controller waits for a short time; the controller controls the telescopic rod 401, the electromagnetic valve 202, the acid regulator 203 and the alkali regulator 206 to act, so that the PH value of the liquid in the transverse tube changes according to the PH value time sequence; after the PH value time sequence is completed, the telescopic rod 401 is controlled to act so as to enable the horizontal pipe to be horizontal, the two electromagnetic valves 202 are sequentially opened, and the solution in the two cups 103 sequentially flows out of the liquid discharge pipe 207; collecting liquid flowing out from the liquid discharge pipe 207 by using the same container, continuously adjusting the pH value to 7 after mixing acid and alkali, and drying to obtain the solution containing dissolved proteins; the one-sided elbow is removed and the small pieces of bone or shell are removed, i.e., the material 301 in the transverse tube is removed, and protein extraction from the bone or shell continues using conventional methods.
Referring to fig. 4, a method for changing the PH of a liquid in a transverse tube according to a PH time sequence includes: the controller controls the telescopic rod 401 to act, so that the cup 103 at one side is lifted, the electromagnetic valve 202 is opened, and an acid solution or an alkali solution corresponding to the PH value time sequence flows into the transverse pipe and then flows into the transverse pipe; the pH value of the liquid can be measured and obtained by the acid regulator 203 and the alkali regulating machine, the controller calculates the acid dosage or the alkali dosage required for obtaining the regulating target pH value, and the acid dosage or the alkali dosage is injected into the transverse tube by the corresponding acid regulator 203 or the alkali regulator 206; the solution finally flows into the transverse tube from the cup 103 with higher position, covers the sedimentation part of the transverse tube and closes the electromagnetic valve 202; controlling the telescopic rod 401 to enable the transverse tube to be horizontal, and standing until the PH value time sequence reaches the moment of changing the PH value; if the acid-base property is not changed, the controller calculates the acid dosage or the alkali dosage required for obtaining the adjustment target PH value, and the acid dosage or the alkali dosage is injected into the transverse pipe by the corresponding acid adjuster 203 or the alkali adjuster 206; if the acid-base property is changed, the controller controls the telescopic rod 401 to act, so that the cup 103 without the solution is lowered, the electromagnetic valve 202 with a lower position is opened, and after all the solution in the transverse tube flows into the cup 103, the electromagnetic valve 202 is closed; the electromagnetic valve 202 at the bent pipe with higher position is opened, and the solution in the cup 103 with higher position flows into the transverse pipe, so that the acid-base property in the transverse pipe is rapidly changed; both the acid regulator 203 and the base regulator machine can measure the PH of the resulting liquid, and the controller calculates the acid or base dosage required to achieve the target PH adjustment, which is injected into the cross tube by the corresponding acid regulator 203 or base regulator 206.
For example, the transverse tube is currently in an alkaline environment, and the PH value is equal to 10. In the PH time series, if the PH is required to be adjusted to 9, a part of acid is added through the acid regulator 203, and feedback of PH change is obtained through the PH sensor 503 in the acid regulator 203 or the base regulator 206, so that the PH can be accurately adjusted to 9. Then the pH value needs to be adjusted to 4, namely the solution in the transverse tube is changed to be acidic. The telescopic rod 401 is controlled to operate, and the alkali solution in the horizontal tube is first flowed into the cup 103 on one side. In this embodiment, the left cup 103 is easy to hold acid, and the right cup 103 holds alkali solution. At this time, the position of the right cup 103 is adjusted downward, the corresponding solenoid valve 202 is opened, and the alkaline solution flows into the right cup 103. After all flows in, the solenoid valve 202 is closed. The solenoid valve 202 on the other side is then opened and the acid in the left cup 103 will easily flow into the cross tube. If the PH in the left cup 103 is 5, the PH is further lowered by adding an acid agent through the acid regulator 203 after flowing into the horizontal pipe. In this embodiment, the acid tank 204 is filled with HCL solution, and the alkali tank 205 is filled with NaOH solution.
The beneficial technical effects of this embodiment are: the acid solution can be quickly replaced by the alkali solution, so that the protein is placed under the pH value deviation with high pH value change rate, the structure of the protein can be more effectively changed, the solubility of the protein is increased, and the extraction is convenient; when the acid and alkali of the solution are replaced, the acid solution or the alkali solution is firstly extracted, and the neutralization of the acid and the alkali is not needed, so that the efficiency of pH value change is improved, the research of pH value deviation on protein property change is facilitated, and the acid-alkali solution can be saved; the pH value of the solution in the transverse tube can be conveniently and accurately adjusted by the improved acid adjuster 203 and the alkali adjuster 206.
Embodiment two:
an apparatus for extracting proteins from bones or shells using a PH shift technique, the present embodiment provides specific improvements to the acid regulator 203 and the base regulator 206 on the basis of the first embodiment. Referring to fig. 5, the acid regulator 203 and the alkali regulator 206 each include a regulating sleeve 504, two PH value sensors 503 and a regulating tube 501, the regulating sleeve 504 is sleeved on the cross tube, the cross tube is hollow corresponding to the position of the regulating sleeve 504, the two PH value sensors 503 are respectively installed at two ends of the regulating sleeve 504, the regulating tube 501 is installed on the regulating sleeve 504, the front end of the regulating tube 501 is communicated with the cross tube, the regulating tube 501 includes a cylinder, a driving spring 506, an electronic switch, a slider 502, a unidirectional valve flap, an injection tube and a connecting tube 506, the injection tube and the connecting tube 506 are all installed at the front end of the cylinder, the injection tube is communicated with the cross tube, the connecting tube 506 of the two regulating tubes is communicated with the acid tank 204 or the alkali tank 205, unidirectional valve flaps are all arranged in the injection tube and the connecting tube 506, the slider 502 is installed in the cylinder, the outer wall of the slider 502 is abutted against the inner wall of the cylinder, the driving spring 506 is installed in the cylinder, the driving spring 506 is located between the slider 502 and the front end of the cylinder, the driving spring 506 is fixedly connected with the slider 502, the two ends of the driving spring 506 are respectively connected with the electronic switch and the direct current power supply, the PH value sensor and the electronic switch are connected with the controller in series.
By controlling the on duty cycle of the electronic switch, the amount of contraction of the drive spring 506 can be controlled, i.e., the position of the slider 502 can be controlled. When the slide block 502 is retreated, the acid agent or the alkali agent in the acid tank 204 or the alkali tank 205 flows into the cylinder, and when the slide block 502 is advanced, the acid agent or the alkali agent in the cylinder is added into the transverse pipe, so that the pH value of the liquid in the transverse pipe is changed. The PH value of the liquid in the transverse tube is detected by the PH value sensor 503, and the volume of the liquid can be calculated by combining the cross-sectional area of the transverse tube, the detection period and the liquid flow rate, and the amount of the acid agent or the alkali agent to be added can be calculated according to the volume and the value of the liquid. The PH sensor 503 continues to obtain feedback during the adjustment process, so as to achieve PH adjustment with preset accuracy.
The above-described embodiment is only a preferred embodiment of the present invention, and is not limited in any way, and other variations and modifications may be made without departing from the technical aspects set forth in the claims.
Claims (5)
1. An apparatus for extracting proteins from bone or shell using pH shift technology, characterized in that,
the device comprises a base, two telescopic rods, a transverse pipe, two bent pipes, two containing cups, two electromagnetic valves, an acid regulator, an alkali regulator, an acid tank, an alkali tank, a timer and a controller, wherein the two containing cups are respectively connected to two ends of the transverse pipe through the two bent pipes, the two electromagnetic valves are respectively arranged on the two bent pipes, the two telescopic rods are rotationally connected with the middle part of the transverse pipe, the telescopic rods are arranged on the base, a sedimentation part is arranged in the middle of the transverse pipe, a liquid discharge pipe is arranged at the bottom of the sedimentation part, an electric control valve is arranged on the liquid discharge pipe, the acid regulator and the alkali regulator are respectively arranged on two sides of the transverse pipe, the acid tank and the alkali tank are respectively connected with the acid regulator and the alkali regulator, and the timer, the electromagnetic valves, the electric control valve, the telescopic rods, the acid regulator and the alkali regulator can be all connected with the controller;
the acid regulator and the alkali regulator comprise regulating sleeves, two PH value sensors and a regulating cylinder, wherein the regulating sleeves are sleeved on the transverse pipes, the transverse pipes are hollow corresponding to the positions of the regulating sleeves, the two PH value sensors are respectively installed at two ends of the regulating sleeves, the regulating cylinder is installed on the regulating sleeves, the front ends of the regulating cylinders are communicated with the transverse pipes, the regulating cylinder comprises a cylinder body, a driving spring, an electronic switch, a sliding block, a unidirectional valve clack, an injection pipe and a connecting pipe, the injection pipe and the connecting pipe are all installed at the front ends of the cylinder body, the injection pipe is communicated with the transverse pipes, the connecting pipe of the two regulating pipes is communicated with an acid tank or an alkali tank, unidirectional valve clacks are arranged in the injection pipe and the connecting pipe, the sliding block is installed in the cylinder body, the outer wall of the sliding block is abutted to the inner wall of the cylinder body, the driving spring is installed in the cylinder body, the driving spring is located between the sliding block and the front ends of the cylinder body, the two ends of the driving spring are respectively fixedly connected with the front ends of the cylinder body and the sliding block, the two ends of the driving spring are connected with the electronic switch and the direct-current power supply in series through wires, and the PH value sensor and the electronic switch are connected with the controller;
the contraction amount of the driving spring can be controlled by controlling the on duty ratio of the electronic switch, namely the position of the sliding block can be controlled; when the sliding block retreats, the acid agent or the alkali agent in the acid tank or the alkali tank flows into the cylinder body, and when the sliding block advances, the acid agent or the alkali agent in the cylinder body is added into the transverse pipe, so that the pH value of liquid in the transverse pipe is changed; the PH value of the liquid in the transverse tube is detected by the PH value sensor, and the volume of the liquid can be calculated by combining the cross section area of the transverse tube, the detection period and the liquid flow rate, and the amount of the acid agent or the alkali agent to be added can be calculated according to the volume and the value of the liquid; and in the adjustment process, feedback is obtained through the PH value sensor, so that PH value adjustment with preset accuracy can be achieved.
2. The apparatus of claim 1, wherein the cup is provided with a vent valve and a port.
3. An apparatus for extracting proteins from bone or shell using PH shifting techniques according to claim 1 or 2, wherein the elbow is divided into two parts, the two parts being detachably connected by a sealing interface.
4. A method for extracting proteins from bones or shells using the pH shift technique, which is characterized in that the device for extracting proteins from bones or shells using the pH shift technique according to any one of claims 1 to 3,
comprising the following steps: breaking bones or shells into small blocks, disassembling a bent pipe at one side, loading the small blocks of bones or shells into a settlement part of a transverse pipe, installing the bent pipe, and checking and sealing;
adding an acid solution with a preset pH value into one side containing cup, and adding an alkali solution with a preset pH value into the other side containing cup;
setting a PH value time sequence, inputting the PH value time sequence into a controller, and starting a timer after the controller waits for a short time;
the controller controls the telescopic rod, the electromagnetic valve, the acid regulator and the alkali regulator to act, so that the PH value of the liquid in the transverse tube changes according to the PH value time sequence;
after the PH value time sequence is completed, the telescopic rod is controlled to act so as to enable the horizontal pipe to be horizontal, and the two electromagnetic valves are sequentially opened so as to enable the solution in the two containing cups to sequentially flow out of the liquid discharge pipe;
collecting liquid flowing out from a liquid discharge pipe by using the same container, continuously adjusting the pH value to 7 after mixing acid and alkali, and drying to obtain the solution containing dissolved proteins;
the one-sided elbow is removed, the small piece of bone or shell is removed, and protein extraction from the bone or shell is continued using conventional methods.
5. The method of extracting protein from bone or shell using PH shift technology as claimed in claim 4, wherein the method of varying PH of the fluid in the lateral tube in PH time series comprises:
the controller controls the telescopic rod to act, so that the cup at one side is lifted, the electromagnetic valve is opened, and the acid solution or the alkali solution corresponding to the PH value time sequence flows into the transverse pipe and then flows into the transverse pipe;
the pH value of the liquid can be measured and obtained by the acid regulator and the alkali regulator, the controller calculates the acid dosage or the alkali dosage required by regulating the target pH value, and the acid dosage or the alkali dosage is injected into the transverse tube by the corresponding acid regulator or the alkali regulator;
the solution finally flows into the transverse tube from the cup with higher position, covers the sedimentation part of the transverse tube and closes the electromagnetic valve;
controlling the telescopic rod to enable the horizontal pipe to be horizontal, and standing until the PH value time sequence reaches the moment of changing the PH value;
if the acid-base property is not changed, the controller calculates the acid dosage or the alkali dosage required by regulating the target PH value, and the acid dosage or the alkali dosage is injected into the transverse tube through the corresponding acid regulator or the alkali regulator;
if the acid-base property is changed, the controller controls the telescopic rod to act, so that the cup without solution is lowered, the electromagnetic valve with a lower position is opened, and the electromagnetic valve is closed after all the solution in the transverse tube flows into the cup;
opening the electromagnetic valve at the bent pipe with higher position, and enabling the solution in the cup with higher position to flow into the transverse pipe, so that the acid-base property in the transverse pipe is rapidly changed;
the pH value of the liquid can be measured and obtained by the acid regulator and the alkali regulator, the controller calculates the acid dosage or the alkali dosage required for obtaining the target pH value, and the acid dosage or the alkali dosage is injected into the transverse tube by the corresponding acid regulator or the alkali regulator.
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