CN114276407A

CN114276407A - Device and method for extracting protein from bone or shell by using PH shift technology

Info

Publication number: CN114276407A
Application number: CN202111628091.XA
Authority: CN
Inventors: 唐振兴
Original assignee: Tourism College Of Zhejiang
Current assignee: Tourism College Of Zhejiang
Priority date: 2021-12-29
Filing date: 2021-12-29
Publication date: 2022-04-05
Anticipated expiration: 2041-12-29
Also published as: CN114276407B

Abstract

The invention relates to the technical field of protein treatment equipment, in particular to equipment and a method for extracting protein from bones or shells by adopting a PH offset technology. The substantial effects of the invention are as follows: can more effectively change the structure of the protein, increase the solubility of the protein and facilitate the extraction of the protein.

Description

Device and method for extracting protein from bone or shell by using PH shift technology

The invention relates to the technical field of protein processing equipment, in particular to equipment and a method for extracting protein from bones or shells by adopting a PH shift technology.

Background

The shell mainly comprises an inorganic phase and an organic phase, wherein the inorganic phase is about 95-99.9% of CaCO 3. The organic phase consists of about 0.1-5% of organic matters, proteins, glycoproteins, polysaccharides, chitin, lipids and the like, and mainly comprises an acid-soluble component, an acid-insoluble-denaturant-soluble component and an acid-insoluble-denaturant-insoluble component. Research shows that the organic matrix of shell has signal molecules capable of promoting cell osteogenic differentiation, and these signal factors can activate the activity of cell alkaline phosphatase, promote the expression of some specific protein and gene in the cell osteogenic differentiation process, induce cell mineralization, etc. so that the shell structure has excellent biocompatibility in vivo. Research finds that human bone marrow stromal cells grow on the pearl layer human bone material and secrete cell stroma, and the pearl layer-polylactic acid composite artificial bone material has no obvious influence on the proliferation of the bone marrow stromal cells and shows good biocompatibility. Therefore, based on the biocompatibility of the shell, the method can be used for the research of the shell-based biological material. Therefore, the method has important significance for the research of the protein of the shell and the bone. However, there is no technical solution for extracting protein from bone or shell with high efficiency. The PH shift technique is a recently emerging protein modification technique that is capable of altering a variety of properties of proteins, including solubility. Therefore, there is a need to develop a technique for extracting proteins based on the PH shift technique.

For example, Chinese patent CN111053145A, published as 2020, 4, 24, discloses a method for improving solubility of potato protein by ultrasonic and pH shift, belonging to the technical field of protein modification. Which comprises the following steps: (1) peeling potato, cutting into pieces, and soaking in 0.12% sodium sulfite solution; (2) standing the potato homogenate for 15min, centrifuging to obtain a supernatant, adjusting the pH to 8.0, stirring and leaching at room temperature for 2h, centrifuging to obtain a supernatant, adjusting the pH to 4.0, standing for 1h, re-dissolving the precipitate with distilled water, adjusting the pH to 7.0, and freeze-drying to obtain potato powder; (3) preparing the obtained potato protein into proper concentration for ultrasonic treatment; (4) and (4) adjusting the pH of the protein solution subjected to ultrasonic treatment in the step (3) to 12.0, maintaining for 1h, and adjusting the pH back to 7.0 to obtain a modified potato protein solution. The modification method provided by the technical scheme can greatly improve the solubility of the potato protein and has very important significance for expanding the application field of the potato protein. However, the solution is not suitable for increasing the solubility of proteins in bone and shell.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: the technical problem of the lack of a scheme for efficiently extracting proteins from bones or shells is present. An apparatus and method for extracting proteins from bone or shell using PH shift technology is presented that can be used to study what PH shift will be most effective in extracting proteins from bone or shell.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows: the utility model provides an adopt PH skew technique to draw equipment of protein in bone or shell, includes base, two telescopic links, violently pipe, two return bends, two appearance cups, two solenoid valves, acid regulator, alkali regulator, acid tank, alkali case, time-recorder and controller, and two appearance cups are connected at violently pipe both ends through two return bends respectively, and two solenoid valves are installed respectively on two return bends, two the telescopic link rotates with violently pipe middle part and is connected, and the telescopic link is installed on the base, is equipped with the portion of sinking in the middle of violently pipe, the portion bottom of subsiding is equipped with the fluid-discharge tube, install the automatically controlled valve on the fluid-discharge tube, and acid regulator and alkali regulator are installed respectively in violently pipe both sides, and acid tank and alkali case are connected with acid regulator and alkali regulator respectively, acid regulator and alkali regulator homoenergetic detect and adjust the PH value of violently intraductal liquid, time-recorder, solenoid valve, automatically controlled valve, telescopic link, alkali regulator, The acid regulator and the alkali regulator are both connected with the controller.

Preferably, the containing cup is provided with a vent valve and a liquid filling opening.

Preferably, the elbow is divided into two parts, and the two parts are detachably connected through a sealing interface.

Preferably, the acid regulator and the alkali regulator both comprise regulating sleeves, two pH value sensors and a regulating cylinder, the regulating sleeves are sleeved on the transverse pipes, the transverse pipes are hollowed corresponding to the positions of the regulating sleeves, the two pH value sensors are respectively installed at the two ends of the regulating sleeves, the regulating cylinders are installed on the regulating sleeves, the front ends of the regulating cylinders are communicated with the transverse pipes, each regulating cylinder comprises a cylinder body, a driving spring, an electronic switch, a sliding block, a one-way valve clack, an injection pipe and a connecting pipe, the injection pipe and the connecting pipe are both installed at the front end of the cylinder body, the injection pipe is communicated with the transverse pipes, the connecting pipes of the two regulating pipes are communicated with the acid box or the alkali box, the one-way valve clack is arranged in the injection pipe and the connecting pipe, the sliding block is installed in the cylinder body, the outer wall of the sliding block is abutted against the inner wall of the cylinder body, the driving spring is installed in the cylinder body, and the driving spring is located between the sliding block and the front end of the cylinder body, two ends of the driving spring are respectively and fixedly connected with the front end of the cylinder body and the sliding block, two ends of the driving spring are connected with the electronic switch and the direct-current power supply in series through wires, and the PH value sensor and the electronic switch are connected with the controller.

A method of extracting protein from bone or shell using PH shift technology, using an apparatus as described above for extracting protein from bone or shell using PH shift technology, comprising: breaking bones or shells into small pieces, disassembling an elbow pipe at one side, loading the small pieces of bones or shells into a sinking part of a transverse pipe, installing the elbow pipe, and checking and sealing; adding acid liquor with a preset pH value into the cup at one side, and adding alkali liquor with a preset pH value into the cup at the other side; a PH value time sequence is formulated and input into a controller, and the controller starts a timer after waiting for a short time; the controller controls the telescopic rod, the electromagnetic valve, the acid regulator and the alkali regulator to act, so that the pH value of the liquid in the transverse pipe changes according to the pH value time sequence; after the PH value time sequence is completed, the telescopic rod is controlled to act to enable the transverse pipe to be horizontal, and the two electromagnetic valves are opened in sequence to enable the solutions in the two containing cups to flow out of the liquid discharge pipe in sequence; collecting liquid flowing out of a liquid discharge pipe by using the same container, continuously adjusting the pH value to 7 after acid-base mixing, and drying the solution which contains dissolved protein to obtain the protein-containing water-soluble protein emulsion; the elbow on one side is removed, the small bone or shell is removed, and the protein extraction from the bone or shell is continued using conventional methods.

Preferably, the method for changing the PH of the liquid in the transverse tube in a time-series PH comprises: the controller controls the telescopic rod to move, so that the cup at one side is lifted, the electromagnetic valve is opened, and the acid solution or the alkali solution corresponding to the PH value time sequence flows into the transverse pipe and further flows into the transverse pipe; the acid regulator and the alkali regulator can measure and obtain the pH value of the liquid, the controller calculates the acid dosage or the alkali dosage required by obtaining the target pH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator or the corresponding alkali regulator; the solution finally flows into the transverse pipe from the higher containing cup, covers the sinking part of the transverse pipe and closes the electromagnetic valve; controlling the telescopic rod to enable the transverse pipe to be horizontal, and standing until the PH value time sequence reaches the time of changing the PH value; if the acid-base property is not changed, the controller calculates the acid dosage or the alkali dosage required by the adjustment of the target PH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator or the corresponding alkali regulator; if the acid-base property is changed, the controller controls the telescopic rod to act, so that the containing cup without the solution is lowered, the electromagnetic valve with a lower position is opened, and the electromagnetic valve is closed after the solution in the transverse pipe completely flows into the containing cup; opening the electromagnetic valve at the elbow with higher position, and enabling the solution in the containing cup with higher position to flow into the transverse pipe, so that the pH value in the transverse pipe is rapidly changed; the acid regulator and the alkali regulator can measure and obtain the pH value of the liquid, the controller calculates the acid dosage or the alkali dosage required for obtaining the target pH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator or the corresponding alkali regulator.

The substantial effects of the invention are as follows: the acid solution can be quickly replaced by the alkali solution, so that the protein is placed under the pH value deviation with high pH value change rate, the structure of the protein can be more effectively changed, the solubility of the protein is increased, and the extraction is convenient; when the pH value of the solution is changed, the acid solution or the alkali solution is extracted, and the acid and alkali neutralization is not needed, so that the change efficiency of the pH value is accelerated, the study of the pH value deviation on the change of the protein property is facilitated, and the acid and alkali solution can be saved; through the improved acid regulator and the improved alkali regulator, the pH value of the solution in the transverse pipe can be conveniently and accurately regulated.

Drawings

FIG. 1 is a schematic diagram of an apparatus for extracting protein according to an embodiment.

FIG. 2 is a schematic diagram of an apparatus for extracting protein according to an embodiment.

FIG. 3 is a schematic diagram of a method for extracting protein from bone or shell according to an embodiment

FIG. 4 is a diagram illustrating a method for adjusting pH in a cross-tube according to an embodiment.

FIG. 5 is a schematic diagram of an embodiment diacid regulator.

Wherein: 101. the device comprises a ventilation valve, 102, a filling opening, 103, a containing cup, 201, a sealing interface, 202, an electromagnetic valve, 203, an acid regulator, 204, an acid box, 205, an alkali box, 206, an alkali regulator, 207, a liquid discharge pipe, 301, a material, 401, a telescopic rod, 402, a base, 501, an adjusting cylinder, 502, a sliding block, 503, a pH value sensor, 504, an adjusting sleeve, 505, a driving spring, 506 and a connecting pipe.

Detailed Description

The following provides a more detailed description of the present invention, with reference to the accompanying drawings.

The first embodiment is as follows:

an apparatus for extracting protein from bone or shell by using PH shift technology, please refer to attached figure 1 and attached figure 2, comprising a base 402, two telescopic pipes 401, a horizontal pipe, two bent pipes, two containing cups 103, two electromagnetic valves 202, an acid regulator 203, an alkali regulator 206, an acid tank 204, an alkali tank 205, a timer and a controller, wherein the two containing cups 103 are respectively connected with two ends of the horizontal pipe through the two bent pipes, the two electromagnetic valves 202 are respectively arranged on the two bent pipes, the two telescopic pipes 401 are rotatably connected with the middle part of the horizontal pipe, the telescopic pipes 401 are arranged on the base 402, a sinking part is arranged in the middle of the horizontal pipe, a drain pipe 207 is arranged at the bottom of the sinking part, an electric control valve is arranged on the drain pipe 207, the acid regulator 203 and the alkali regulator 206 are respectively arranged at two sides of the horizontal pipe, the acid tank 204 and the alkali tank 205 are respectively connected with the acid regulator 203 and the alkali regulator 206, the acid regulator 203 and the alkali regulator 206 can detect and regulate the PH value of the liquid in the horizontal pipe, the timer, the electromagnetic valve 202, the electric control valve, the telescopic rod 401, the acid regulator 203 and the alkali regulator 206 are all connected with the controller. The containing cup 103 is provided with a vent valve 101 and a filling opening 102. The elbow is divided into two parts which are detachably connected through a sealing interface 201. The filling opening 102 can be opened and closed, and is opened during filling, and the filling opening 102 is closed after filling is completed.

A method of extracting protein from bone or shell using PH shift technology, using an apparatus as described above for extracting protein from bone or shell using PH shift technology, with reference to fig. 3, comprising: breaking bones or shells into small pieces, disassembling bent pipes on one side, loading the small pieces of bones or shells into a settling part of a transverse pipe, namely loading the material 301 into the settling part of the transverse pipe, installing the bent pipes and checking and sealing; adding acid liquor with a preset pH value into the cup 103 at one side, and adding alkali liquor with a preset pH value into the cup 103 at the other side; a PH value time sequence is formulated and input into a controller, and the controller starts a timer after waiting for a short time; the controller controls the telescopic rod 401, the electromagnetic valve 202, the acid regulator 203 and the alkali regulator 206 to act, so that the pH value of the liquid in the transverse pipe changes according to the pH value time sequence; after the PH value time sequence is completed, the telescopic rod 401 is controlled to move to enable the transverse pipe to be horizontal, the two electromagnetic valves 202 are opened in sequence, and the solutions in the two containing cups 103 flow out of the liquid discharge pipe 207 in sequence; collecting liquid flowing out of the liquid discharge pipe 207 by using the same container, continuously adjusting the pH value to 7 after acid-base mixing, and drying the solution which contains dissolved protein to obtain the protein-containing water-soluble protein emulsion; the bent tube at one side is disassembled, and the small bone or shell is taken out, namely the material 301 in the transverse tube is taken out, and the protein is continuously extracted from the bone or shell by using the conventional method.

Referring to fig. 4, the method for changing the PH of the liquid in the transverse tube according to the time-series PH includes: the controller controls the telescopic rod 401 to move, so that the cup 103 on one side is lifted, the electromagnetic valve 202 is opened, and the acid solution or the alkali solution corresponding to the PH value time sequence flows into the transverse pipe and further flows into the transverse pipe; the acid regulator 203 and the alkali regulator can measure and obtain the pH value of the liquid, the controller calculates the acid dosage or the alkali dosage required by obtaining the target pH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator 203 or the corresponding alkali regulator 206; the solution finally flows into the horizontal pipe from the higher cup 103, covers the horizontal pipe settling part and closes the electromagnetic valve 202; controlling the telescopic rod 401 to enable the transverse pipe to be horizontal, and standing until the PH value time sequence reaches the time of changing the PH value; if the acid-base property is not changed, the controller calculates the acid dosage or the alkali dosage required for adjusting the target pH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator 203 or the corresponding alkali regulator 206; if the acid-base property is changed, the controller controls the telescopic rod 401 to act, so that the containing cup 103 without the solution is lowered, the electromagnetic valve 202 with the lower position is opened, and the electromagnetic valve 202 is closed after the solution in the transverse pipe completely flows into the containing cup 103; the electromagnetic valve 202 at the elbow with higher position is opened, and the solution in the containing cup 103 with higher position flows into the horizontal pipe, so that the pH value in the horizontal pipe is rapidly changed; the acid regulator 203 and the alkali regulator can measure and obtain the pH value of the liquid, the controller calculates the acid dosage or the alkali dosage required for obtaining the target pH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator 203 or the corresponding alkali regulator 206.

For example, the inside of the cross-tube is currently in an alkaline environment with a PH equal to 10. In the PH time series, if the PH needs to be adjusted to 9, the PH can be accurately adjusted to 9 by adding part of the acid through the acid adjustor 203 and obtaining the feedback of the PH change through the PH sensor 503 in the acid adjustor 203 or the alkali adjustor 206. The PH then needs to be adjusted to 4, i.e. the solution in the transverse tube is changed to acidic. The telescopic rod 401 is controlled to move, and the alkaline solution in the horizontal pipe is firstly flowed into the cup 103 on one side. In this embodiment, the left cup 103 can easily contain acid, and the right cup 103 can contain alkaline solution. At this time, the position of the right cup 103 is adjusted downward, the corresponding solenoid valve 202 is opened, and the alkaline solution flows into the right cup 103. After all the inflow, the solenoid valve 202 is closed. Then the solenoid valve 202 on the other side is opened, and the acid in the cup 103 on the left side is easy to flow into the transverse pipe. When the PH in the left cup 103 is 5, the acid is added by the acid adjuster 203 after flowing into the horizontal pipe, and the PH is further lowered. In this example, the acid tank 204 is filled with an HCL solution, and the base tank 205 is filled with an NaOH solution.

The beneficial technical effects of this embodiment are: the acid solution can be quickly replaced by the alkali solution, so that the protein is placed under the pH value deviation with high pH value change rate, the structure of the protein can be more effectively changed, the solubility of the protein is increased, and the extraction is convenient; when the pH value of the solution is changed, the acid solution or the alkali solution is extracted, and the acid and alkali neutralization is not needed, so that the change efficiency of the pH value is accelerated, the study of the pH value deviation on the change of the protein property is facilitated, and the acid and alkali solution can be saved; the pH value of the solution in the transverse pipe can be conveniently and accurately adjusted by the improved acid adjuster 203 and the improved alkali adjuster 206.

Example two:

the present embodiment is based on the first embodiment and specifically improves the acid regulator 203 and the alkali regulator 206. Referring to fig. 5, the acid regulator 203 and the alkali regulator 206 each include a regulating sleeve 504, two PH sensors 503, and a regulating cylinder 501, the regulating sleeve 504 is sleeved on a horizontal pipe, the horizontal pipe is hollow corresponding to the regulating sleeve 504, the two PH sensors 503 are respectively installed at two ends of the regulating sleeve 504, the regulating cylinder 501 is installed on the regulating sleeve 504, the front end of the regulating cylinder 501 is communicated with the horizontal pipe, the regulating cylinder 501 includes a cylinder body, a driving spring 506, an electronic switch, a sliding block 502, a one-way valve flap, an injection pipe, and a connecting pipe 506, the injection pipe and the connecting pipe 506 are both installed at the front end of the cylinder body, the injection pipe is communicated with the horizontal pipe, the connecting pipe 506 of the two regulating pipes is communicated with the acid tank 204 or the alkali tank 205, the one-way valve flap is respectively installed in the injection pipe and the connecting pipe 506, the sliding block 502 is installed in the cylinder body, the outer wall of the sliding block 502 is abutted against the inner wall of the cylinder body, the driving spring 506 is installed in the cylinder body, the driving spring 506 is located between the sliding block 502 and the front end of the cylinder body, two ends of a driving spring 506 are respectively and fixedly connected with the front end of the cylinder body and the sliding block 502, two ends of the driving spring 506 are connected with an electronic switch and a direct current power supply in series through conducting wires, and the pH value sensor 503 and the electronic switch are connected with a controller.

By controlling the on duty of the electronic switch, the amount of contraction of the drive spring 506, i.e., the position of the slider 502, can be controlled. When the slide block 502 retreats, the acid agent or the alkali agent in the acid box 204 or the alkali box 205 flows into the cylinder, and when the slide block 502 advances, the acid agent or the alkali agent in the cylinder is added into the horizontal pipe, so that the pH value of the liquid in the horizontal pipe is changed. The pH value of the liquid in the transverse pipe is detected by the pH value sensor 503, the volume of the liquid can be calculated by combining the cross section area of the transverse pipe, the detection period and the liquid flow rate, and the amount of the acid agent or the alkali agent to be added can be calculated according to the volume and the value of the liquid. In the adjusting process, feedback is continuously obtained through the pH value sensor 503, and the pH value adjustment with preset accuracy can be achieved.

The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.

Claims

1. An apparatus for extracting protein from bone or shell using pH shift technology,

including base, two telescopic links, violently pipe, two return bends, two appearance cups, two solenoid valves, sour regulator, alkali regulator, acid tank, alkali tank, time-recorder and controller, two appearance cups are connected at violently pipe both ends through two return bends respectively, and two solenoid valves are installed respectively on two return bends, two the telescopic link rotates with violently managing the middle part and is connected, and the telescopic link is installed on the base, violently is equipped with the portion of subsiding in the middle of the pipe, the portion bottom of subsiding is equipped with the fluid-discharge tube, install automatically controlled valve on the fluid-discharge tube, sour regulator and alkali regulator are installed respectively in violently managing both sides, and acid tank and alkali tank are connected with sour regulator and alkali regulator respectively, sour regulator and alkali regulator homoenergetic detect and adjust the PH value of violently intraductal liquid, and time-recorder, solenoid valve, automatically controlled valve, telescopic link, sour regulator and alkali regulator all are connected with the controller.

2. The apparatus for extracting protein from bone or shell using pH shift technology according to claim 1,

the containing cup is provided with a vent valve and a liquid feeding port.

3. The apparatus for extracting protein from bone or shell using pH shift technology according to claim 1 or 2,

the elbow is divided into two parts, and the two parts are detachably connected through a sealing interface.

4. The apparatus for extracting protein from bone or shell using pH shift technology according to claim 1 or 2,

the acid regulator and the alkali regulator respectively comprise regulating sleeves, two PH value sensors and a regulating barrel, the regulating sleeves are sleeved on the transverse pipes, the transverse pipes are hollowed corresponding to the positions of the regulating sleeves, the two PH value sensors are respectively arranged at the two ends of the regulating sleeves, the regulating barrel is arranged on the regulating sleeves, the front ends of the regulating barrels are communicated with the transverse pipes, each regulating barrel comprises a barrel body, a driving spring, an electronic switch, a sliding block, a one-way valve clack, an injection pipe and a connecting pipe, the injection pipe and the connecting pipe are both arranged at the front end of the barrel body, the injection pipe is communicated with the transverse pipes, the connecting pipes of the two regulating pipes are communicated with an acid box or an alkali box, the one-way valve clack is arranged in the injection pipe and the connecting pipe, the sliding block is arranged in the barrel body, the outer wall of the sliding block is abutted against the inner wall of the barrel body, the driving spring is arranged in the barrel body, and the driving spring is arranged between the sliding block and the front end of the barrel body, two ends of the driving spring are respectively and fixedly connected with the front end of the cylinder body and the sliding block, two ends of the driving spring are connected with the electronic switch and the direct-current power supply in series through wires, and the PH value sensor and the electronic switch are connected with the controller.

5. A method for extracting proteins from bones or shells by using the pH shift technique, using the apparatus for extracting proteins from bones or shells by using the pH shift technique according to any one of claims 1 to 4,

the method comprises the following steps:

breaking bones or shells into small pieces, disassembling an elbow pipe at one side, loading the small pieces of bones or shells into a sinking part of a transverse pipe, installing the elbow pipe, and checking and sealing;

adding acid liquor with a preset pH value into the cup at one side, and adding alkali liquor with a preset pH value into the cup at the other side;

a PH value time sequence is formulated and input into a controller, and the controller starts a timer after waiting for a short time;

the controller controls the telescopic rod, the electromagnetic valve, the acid regulator and the alkali regulator to act, so that the pH value of the liquid in the transverse pipe changes according to the pH value time sequence;

after the PH value time sequence is completed, the telescopic rod is controlled to act to enable the transverse pipe to be horizontal, and the two electromagnetic valves are opened in sequence to enable the solutions in the two containing cups to flow out of the liquid discharge pipe in sequence;

collecting liquid flowing out of a liquid discharge pipe by using the same container, continuously adjusting the pH value to 7 after acid-base mixing, and drying the solution which contains dissolved protein to obtain the protein-containing water-soluble protein emulsion;

the elbow on one side is removed, the small bone or shell is removed, and the protein extraction from the bone or shell is continued using conventional methods.

6. The method of claim 5, wherein the pH shift technique is used to extract proteins from bone or shell,

the method for changing the pH value of the liquid in the transverse pipe according to the time sequence of the pH value comprises the following steps:

the controller controls the telescopic rod to move, so that the cup at one side is lifted, the electromagnetic valve is opened, and the acid solution or the alkali solution corresponding to the PH value time sequence flows into the transverse pipe and further flows into the transverse pipe;

the acid regulator and the alkali regulator can measure and obtain the pH value of the liquid, the controller calculates the acid dosage or the alkali dosage required by obtaining the target pH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator or the corresponding alkali regulator;

the solution finally flows into the transverse pipe from the higher containing cup, covers the sinking part of the transverse pipe and closes the electromagnetic valve;

controlling the telescopic rod to enable the transverse pipe to be horizontal, and standing until the PH value time sequence reaches the time of changing the PH value;

if the acid-base property is not changed, the controller calculates the acid dosage or the alkali dosage required by the adjustment of the target PH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator or the corresponding alkali regulator;

if the acid-base property is changed, the controller controls the telescopic rod to act, so that the containing cup without the solution is lowered, the electromagnetic valve with a lower position is opened, and the electromagnetic valve is closed after the solution in the transverse pipe completely flows into the containing cup;

opening the electromagnetic valve at the elbow with higher position, and enabling the solution in the containing cup with higher position to flow into the transverse pipe, so that the pH value in the transverse pipe is rapidly changed;

the acid regulator and the alkali regulator can measure and obtain the pH value of the liquid, the controller calculates the acid dosage or the alkali dosage required for obtaining the target pH value, and the acid dosage or the alkali dosage is injected into the transverse pipe through the corresponding acid regulator or the corresponding alkali regulator.