CN114426575B - 抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒 - Google Patents
抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒 Download PDFInfo
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Abstract
本发明公开了一种抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒,涉及抗体技术领域。本发明公开的抗新型冠状病毒的抗体包括重链互补决定区和轻链互补决定区。该抗体对新型冠状病毒的N蛋白的亲和力较好,使用该抗体检测新型冠状病毒具有较好的灵敏度和特异性。本发明为新型冠状病毒的检测提供了更为丰富的抗体选择。
Description
本申请是针对申请号为:202011179251.2,(发明名称为:抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒,申请日:2020-10-29)提出的分案申请。
技术领域
本发明涉及抗体技术领域,具体而言,涉及一种抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒。
背景技术
新型冠状病毒2019-nCoV的结构蛋白分为:刺突糖蛋白(S蛋白)、包膜糖蛋白(E蛋白)、膜糖蛋白(M蛋白)和核衣壳蛋白(N蛋白),这些蛋白包括多个抗原表位。N蛋白与病毒基因组RNA相互缠绕形成病毒核衣壳,在病毒RNA的合成过程中发挥着重要的作用。同时,N蛋白相对保守,在病毒的结构蛋白中所占比例最大,感染早期机体就能产生抗N蛋白的高水平抗体。最后,N蛋白是新型冠状病毒重要的标志蛋白,利用抗原与抗体特异性结合的原理,可通过N蛋白单克隆抗体检测抗原的存在,从而直接证明样本中含有新型冠状病毒,实现新型冠状病毒的检测。
检测的抗体主要分为IgM和IgG两类。目前对新型冠状病毒的这两类抗体的产生和持续时间尚缺乏系统性研究。通常情况下,IgM抗体产生早,一经感染,快速产生,维持时间短,消失快,血液中检测阳性可反应机体处于急性感染状态,可作为早期感染的指标。与核酸检测方法相比,抗体检测样本为血清或血浆,受样本采样的影响较小,有利于早期诊断和排除可疑病例,同时检测快速、方便、适合大规模筛查。
新型冠状病毒2019-nCoV肺炎疫情发生以来,在全球蔓延,严重威胁着人类的生命安全和身体健康。呼吸道飞沫和密切接触传播是新型冠状病毒肺炎主要的传播途径,在相对封闭的环境中长时间暴露于高浓度气溶胶情况中存在经气溶胶传播的可能。2019-nCoV具有很强的传染性,多数患者感染后出现临床症状,但也有部分患者为无症状感染者。人感染了冠状病毒后常见体征有呼吸道症状、发热、咳嗽、气促和呼吸困难等。在较严重病例中,感染可导致肺炎、严重急性呼吸综合征、肾衰竭,甚至死亡。虽然目前对于新型冠状病毒所致疾病没有特异治疗方法,但是轻症、或者无症状患者通过治疗可以大大的提高治愈率,缩短治疗时间。因此,对患者的检测或者鉴定变的尤为重要。
目前主要以核酸检测和病毒基因测序为病原学确诊证据,由于采样、操作以及试剂等多方面因素影响,核酸检测会出现假阴性结果。高度疑似2019新型冠状病毒(2019-nCoV)感染患者的病毒核酸阳性检出率仅为30%~50%。另外,核酸检测对仪器设备、检测场地及环境条件要求高,存在检测时间长、通量低等缺点,不便于目前疫情下的人群大规模检测。因此,迫切需要研发出一种快速便捷检测试剂盒用于临床检测,从而尽快隔离感染人群以阻断病毒传播。因此,抗体检测试剂盒变得更为重要。
目前2019-nCoV N蛋白单克隆抗体产品较少,性能存在差异。
鉴于此,特提出本发明。
发明内容
本发明的目的在于提供一种抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒,该抗体可以特异性结合新型冠状病毒的N蛋白,对其具有较高的亲和力,用该抗体检测新型冠状病毒具有较好的灵敏度和特异性。本发明为新型冠状病毒的检测提供了更为丰富的抗体选择。
本发明是这样实现的:
一方面,本发明提供一种抗新型冠状病毒或其N蛋白的抗体或其功能性片段,所述抗体或其功能性片段具有如下互补决定区:
CDR-VH1:G-X1-T-F-T-X2-Y-X3-M-N;其中:X1是Y;X2是N;X3是G;
CDR-VH2:W-X1-N-T-Y-X2-G-E-P-T-Y-A-X3-X4-F;其中:X1是I;X2是T;X3是D;X4是D;
CDR-VH3:A-R-X1-A-X2-X3-R-S-Y;其中:X1是S;X2是L;X3是L;
CDR-VL1:K-A-S-X1-D-X2-S-T-A-X3-A;其中:X1是Q;X2是V;X3是V;
CDR-VL2:W-X1-S-T-R-H-X2;其中:X1是A;X2是T;
CDR-VL3:Q-Q-H-X1-S-T-P-X2;其中:X1是Y;X2是L。
本发明提供的抗新型冠状病毒的抗体或其功能性片段,具有上述互补决定区结构,上述互补决定区结构可使抗体或其功能性片段能够特异性结合抗新型冠状病毒的N蛋白,对其具有较好的亲和力,用该抗体或其功能性片段检测新型冠状病毒,具有较好的特异性和灵敏度。本发明为新型冠状病毒的检测提供了更为丰富的抗体选择。
在可选的实施方式中,所述抗体或其功能性片段与抗新型冠状病毒的N蛋白以KD≤10-9mol/L的亲和力结合。
KD的检测参考本发明实施例中的方法进行。
在可选的实施方式中,所述抗体包括序列依次如SEQ ID NO:1-4所示的轻链骨架区FR1-L、FR2-L、FR3-L及FR4-L,和/或,序列依次如SEQ ID NO:5-8所示的重链骨架区FR1-H、FR2-H、FR3-H及FR4-H。
通常情况下,重链可变区(VH)和轻链的可变区(VL)可由以下编号的CDR与FR按如下组合排列连接获得:FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4。
需要说明的是,在其他的实施例中,本发明提供的抗体或其功能性片段的各骨架区氨基酸序列可以与上述对应骨架区(SEQ ID NO:1、2、3、4、5、6、7或8)可以具有至少80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或99%的同源性。
在可选的实施方式中,所述抗体还包含恒定区。
在可选的实施方式中,所述恒定区选自IgG1、IgG2、IgG3、IgG4、IgA、IgM、IgE和IgD中的任意一者的恒定区。
在可选的实施方式中,所述恒定区的种属来源为牛、马、乳牛、猪、绵羊、山羊、大鼠、小鼠、狗、猫、兔、骆驼、驴、鹿、貂、鸡、鸭、鹅、火鸡、斗鸡或人。
在可选的实施方式中,所述恒定区来源于小鼠。
在可选的实施方式中,所述恒定区的轻链恒定区序列如SEQ ID NO:9所示,所述恒定区的重链恒定区序列如SEQ ID NO:10所示。
在可选的实施方式中,所述功能性片段选自所述抗体的VHH、F(ab’)2、Fab’、Fab、Fv和scFv中的任意一种。
上述抗体的功能性片段通常具有与其来源抗体相同的结合特异性。本领域技术人员根据本发明记载的内容容易理解到,上述抗体的功能性片段可以通过比如酶消化的方法(包括胃蛋白酶或木瓜蛋白酶)和/或通过化学还原分裂二硫键的方法获得。在本发明公开了完整抗体的结构基础上,本领域技术人员容易获得上述的功能性片段。
上述抗体的功能性片段还可以通过也是本领域技术人员所知的重组遗传学技术或通过例如自动肽合成仪,比如Applied BioSystems等销售的自动肽合成仪合成获得。
另一方面,本发明提供一种检测新型冠状病毒或其N蛋白的试剂或试剂盒,其包括如上任一项所述的抗体或其功能性片段。
在可选的实施方式中,上述试剂或试剂盒中所述抗体或其功能性片段标记有可被检测的标记物。
可被检测的标记物是指具有能够被肉眼直接观察或被仪器检测或探测到的特性例如发光、显色、放射性等特性的一类物质,通过该特性可以实现对相应目标物的定性或定量检测。
在可选的实施方式中,所述可被检测的标记物包括但不限于荧光染料、催化底物显色的酶、放射性同位素、化学发光试剂和纳米颗粒类标记物。
在实际的使用过程中,本领域技术人员可以根据检测条件或实际需要选择合适的标记物,无论使用何种标记物,其均属于本发明的保护范围。
在可选的实施方式中,所述荧光染料包括但不限于荧光素类染料及其衍生物(例如包括但不限于异硫氰酸荧光素(FITC)羟基光素(FAM)、四氯光素(TET)等或其类似物)、罗丹明类染料及其衍生物(例如包括但不限于红色罗丹明(RBITC)、四甲基罗丹明(TAMRA)、罗丹明B(TRITC)等或其类似物)、Cy系列染料及其衍生物(例如包括但不限于Cy2、Cy3、Cy3B、Cy3.5、Cy5、Cy5.5、Cy3等或其类似物)、Alexa系列染料及其衍生物(例如包括但不限于AlexaFluor350、405、430、488、532、546、555、568、594、610、33、647、680、700、750等或其类似物)和蛋白类染料及其衍生物(例如包括但不限于藻红蛋白(PE)、藻蓝蛋白(PC)、别藻蓝蛋白(APC)、多甲藻黄素-叶绿素蛋白(preCP)等)。
在可选的实施方式中,所述催化底物显色的酶包括但不限于辣根过氧化物酶、碱性磷酸酶、β-半乳糖苷酶、葡萄糖氧化酶、碳酸酐酶、乙酰胆碱酯酶以及6-磷酸葡萄糖脱氧酶。
在可选的实施方式中,所述放射性同位素包括但不限于212Bi、131I、111In、90Y、186Re、211At、125I、188Re、153Sm、213Bi、32P、94mTc、99mTc、203Pb、67Ga、68Ga、43Sc、47Sc、110mIn、97Ru、62Cu、64Cu、67Cu、68Cu、86Y、88Y、121Sn、161Tb、166Ho、105Rh、177Lu、172Lu和18F。
在可选的实施方式中,所述化学发光试剂包括但不限于鲁米诺及其衍生物、光泽精、甲壳动物荧光素及其衍生物、联吡啶钌及其衍生物、吖啶酯及其衍生物、二氧环乙烷及其衍生物、洛粉碱及其衍生物和过氧草酸盐及其衍生物。
在可选的实施方式中,所述纳米颗粒类标记物包括但不限于纳米颗粒、胶体、有机纳米颗粒、磁性纳米颗粒、量子点纳米颗粒和稀土络合物纳米颗粒。
在可选的实施方式中,所述胶体包括但不限于胶体金属、分散型染料、染料标记的微球和乳胶。
在可选的实施方式中,所述胶体金属包括但不限于胶体金、胶体银和胶体硒。
另一方面,本发明提供一种编码上述抗体或其功能性片段的核酸分子。
另一方面,本发明提供含有上述核酸分子的载体。
另一方面,本发明提供含有上述载体的重组细胞。
另一方面,本发明提供一种制备抗体或其功能性片段的方法,其包括:培养如上所述的重组细胞,从培养产物中分离纯化得到所述抗体或其功能性片段。
在本发明公开了抗体或其功能性片段的氨基酸序列的基础上,本领域技术人员容易想到采用基因工程技术或其他技术(化学合成、杂交瘤细胞)制备得到该抗体或其功能性片段,例如从能够重组表达如上任一项所述的抗体或其功能性片段的重组细胞的培养产物中分离纯化得到该抗体或其功能性片段,这对本领域技术人员来说是容易实现的,基于此,无论采用何种技术制备本发明的抗体或其功能性片段,其均属于本发明的保护范围。
附图说明
为了更清楚地说明本发明实施例的技术方案,下面将对实施例中所需要使用的附图作简单地介绍,应当理解,以下附图仅示出了本发明的某些实施例,因此不应被看作是对范围的限定,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他相关的附图。
图1为实施例1的抗新型冠状病毒的抗体的还原性SDS-PAGE的结果。
具体实施方式
为使本发明实施例的目的、技术方案和优点更加清楚,下面将对本发明实施例中的技术方案进行清楚、完整地描述。实施例中未注明具体条件者,按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者,均为可以通过市售购买获得的常规产品。
除非另有定义,否则本文使用的所有技术和科学术语具有与本公开内容所属领域的普通技术人员通常理解的含义相同的含义。尽管与本文描述的那些方法和材料类似或等同的任何方法和材料都可用于本文的制剂或单位剂量的实践或测试,但现在描述一些方法和材料。除非另有说明,否则本文采用或考虑的技术是标准方法。材料、方法和实例仅是说明性而非限制性的。
除非另外指明,否则实践本发明将采用细胞生物学、分子生物学(包含重组技术)、微生物学、生物化学和免疫学的常规技术,所述常规技术在本领域技术人员的能力范围内。文献中充分解释了这种技术,如《分子克隆:实验室手册(Molecular Cloning:ALaboratory Manual)》,第二版(Sambrook等人,1989);《寡核苷酸合成(OligonucleotideSynthesis)》(M.J.Gait编,1984);《动物细胞培养(Animal Cell Culture)》(R.I.Freshney编,1987);《酶学方法(Methods in Enzymology)》(学术出版社有限公司(Academic Press,Inc.);《实验免疫学手册(Handbook of Experimental Immunology)》(D.M.Weir和C.C.Blackwell编);《哺乳动物细胞用基因转移载体(Gene Transfer Vectors forMammalian Cells)》(J.M.Miller和M.P.Calos编,1987);《当代分子生物学方法(CurrentProtocols in Molecular Biology)》(F.M.Ausubel等人编,1987);《PCR:聚合酶链反应(PCR:The Polymerase Chain Reaction)》(Mullis等人编,1994);以及《当代免疫学方法(Current Protocols in Immunology)》(J.E.Coligan等人编,1991),所述文献中的每个文献均通过引用明确并入本文中。
以下结合实施例对本发明的特征和性能作进一步的详细描述。
实施例1
本实施例中限制性内切酶、Prime Star DNA聚合酶购自Takara公司。MagExtractor-RNA提取试剂盒购自TOYOBO公司。BD SMARTTMRACE cDNA Amplification Kit试剂盒购自Takara公司。pMD-18T载体购自Takara公司。质粒提取试剂盒购自天根公司。引物合成和基因测序由Invitrogen公司完成。
1重组质粒的构建
(1)抗体基因制备
从分泌抗新型冠状病毒N蛋白的抗体的杂交瘤细胞株中提取mRNA,通过RT-PCR方法获得DNA产物,该产物用rTaq DNA聚合酶进行加A反应后插入到pMD-18T载体中,转化到DH5α感受态细胞中,长出菌落后分别取Heavy Chain及Light Chain基因克隆,各4个克隆送基因测序公司进行测序。
(2)抗体可变区基因的序列分析
将上述测序得到的基因序列放在IMGT抗体数据库中进行分析,并利用VNTI11.5软件进行分析确定重链和轻链引物对扩增出的基因都是正确的,其中Light Chain扩增出的基因片段中,VL基因序列为324bp,属于VkII基因家族,其前方有57bp的前导肽序列;HeavyChain引物对扩增出的基因片段中,VH基因序列为357bp,属于VH1基因家族,其前方有57bp的前导肽序列。
(3)重组抗体表达质粒的构建
pcDNATM3.4vector为构建的重组抗体真核表达载体,该表达载体已经引入HindIII、BamHI、EcoRI等多克隆酶切位点,并命名为pcDNA3.4A表达载体,后续简称3.4A表达载体;根据上述pMD-18T中抗体可变区基因测序结果,设计该抗体的VL和VH基因特异性引物,两端分别带有HindIII、EcoRI酶切位点和保护碱基,通过PCR扩增方法扩出0.73kb的Light Chain基因片段和1.42kb的Heavy Chain基因片段。
Heavy Chain和Light Chain基因片段分别采用HindIII/EcoRI双酶切,3.4A载体采用HindIII/EcoRI双酶切,将片段和载体纯化回收后Heavy Chain基因和Light Chain基因分别连接3.4A表达载体中,分别得到Heavy Chain和Light Chain的重组表达质粒。
2稳定细胞株筛选
(1)重组抗体表达质粒瞬时转染CHO细胞,确定表达质粒活性
质粒用超纯水稀释至400ng/ml,调节CHO细胞1.43×107cells/ml于离心管中,100μL质粒与700μL细胞混合,转入电转杯,电转,第3、5、7天取样计数,第7天收样检测。
包被液稀释2019-nCoV N蛋白抗原到1μg/ml,每孔100μL,4℃过夜;次日,洗涤液清洗2次,拍干;加入封闭液(20%BSA+80%PBS),每孔120μL,37℃,1h,拍干;加入稀释后的细胞上清,100μL/孔,37℃,30min(部分上清1h);洗涤液清洗5次,拍干;加入羊抗鼠IgG-HRP,每孔100μL,37℃,30min;洗涤液清洗5次,拍干;加入显色液A液(50μL/孔,含柠檬酸+醋酸钠+乙酰苯胺+过氧化脲),加入显色液B液(50μL/孔,含柠檬酸+EDTA·2Na+TMB+浓HCL),10min;加入终止液(50μL/孔,含EDTA·2Na+浓H2SO4);酶标仪上450nm(参考630nm)处读OD值。结果显示细胞上清稀释1000倍后反应OD仍大于1.0,未加细胞上清孔反应OD小于0.1,表明质粒瞬转后产生的抗体对2019-nCoV N蛋白抗原有活性。
(2)重组抗体表达质粒线性化
准备下述试剂:Buffer 50μL、DNA 100μg/管、PuvⅠ酶10μL、无菌水补至500μL,37℃水浴酶切过夜;先用等体积酚/氯仿/异戊醇(下层)25:24:1,再用氯仿(水相)依次进行抽提;0.1倍体积(水相)3M醋酸钠和2倍体积乙醇冰上沉淀,70%乙醇漂洗沉淀,去除有机溶剂,待乙醇挥发完全用适量的灭菌水进行复融,最后进行浓度的测定。
(3)重组抗体表达质粒稳定转染,加压筛选稳定细胞株
质粒用超纯水稀释至400ng/ml,调节CHO细胞1.43×107cells/ml于离心管中,100μL质粒与700μL细胞混合,转入电转杯,电转,次日计数;25umol/L MSX 96孔加压培养约25天。
显微镜下观察标记长有细胞的克隆孔,并记录汇合度;取培养上清,送样检测;挑选抗体浓度、相对浓度高的细胞株转24孔,3天左右转6孔;3天后保种批培,调整细胞密度0.5×106cells/ml,2.2ml进行批培养,细胞密度0.3×106cells/ml,2ml进行保种;7天6孔批培上清送样检测,挑选抗体浓度及细胞直径较小的细胞株转TPP保种传代。
3重组抗体生产
(1)细胞扩培
细胞复苏之后先在125ml规格的摇瓶中培养,接种体积为30ml,培养基为100%Dynamis培养基,放置于转速120r/min,温度为37℃,二氧化碳为8%的摇床中。培养72h,以50万cells/ml接种密度接种扩培,扩培体积根据生产需求进行计算,培养基为100%Dynamis培养基。之后每72h扩培一次。当细胞量满足生产需求时,严格控制接种密度为50万cells/ml左右进行生产。
(2)摇瓶生产及纯化
摇瓶参数:转速120r/min,温度为37℃,二氧化碳为8%。流加补料:在摇瓶中培养至72h时开始每天补料,HyCloneTM Cell BoostTM Feed 7a每天流加初始培养体积的3%,Feed 7b每天流加量为初始培养体积的千分之一,一直补到第12天(第12天补料)。葡萄糖在第六天补加3g/L。第13天收样。用proteinA亲和层析柱进行亲和纯化。取4μg纯化的抗体进行还原性SDS-PAGE,4μg外来对照抗体作为对照,电泳图如下图1所示,在还原性SDS-PAGE后显示两条带,1条Mr为50KD(重链,SEQ ID NO:14),另一条Mr为28KD(轻链,SEQ ID NO:13)。
实施例2
抗体的性能检测
(1)实施例1抗体及其突变体的活性检测
分析实施例1的抗体(WT)序列,其重链可变区如SEQ ID NO:12所示,其中,其中,重链可变区上的各互补决定区的氨基酸序列如下:
CDR-VH1:G-F(X1)-T-F-T-D(X2)-Y-A(X3)-M-N;
CDR-VH2:W-L(X1)-N-T-Y-S(X2)-G-E-P-T-Y-A-E(X3)-D(X4)-F;
CDR-VH3:A-R-T(X1)-A-I(X2)-L(X3)-R-S-Y;
其轻链可变区如SEQ ID NO:11所示,其中,轻链可变区上的各互补决定区的氨基酸序列如下:
CDR1-VL:K-A-S-E(X1)-D-L(X2)-S-T-A-L(X3)-A;
CDR-VL2:W-G(X1)-S-T-R-H-S(X2);
CDR-VL3:Q-Q-H-W(X1)-S-T-P-L(X2)。
在实施例1的抗新型冠状病毒抗体(WT)基础上,在互补决定区中对于抗体活性有关的位点进行突变,其中,X1、X2、X3、X4均为突变位点。见下表1。
表1与抗体活性有关的突变位点
对表1中的抗体结合活性检测:
包被液(主要成分NaHCO3)稀释2019-nCoV N蛋白抗原到1μg/ml进行微孔板包被,每孔100μl,4℃过夜;次日,洗涤液清洗2次,拍干;加入封闭液(20%BSA+80%PBS),每孔120μl,37℃,1h,拍干;加入稀释后的表1中的单克隆抗体,100μl/孔,37℃,30min-60min;洗涤液清洗5次,拍干;加入羊抗鼠IgG-HRP,每孔100μl,37℃,30min;洗涤液(PBS)清洗5次,拍干;加入显色液A液(50μl/孔,含2.1g/L柠檬酸、12.25g/L柠檬酸、0.07g/L乙酰苯胺和0.5g/L过氧化脲),加入显色液B液(50μl/孔,含1.05g/L柠檬酸、0.186g/LEDTA·2Na、0.45g/LTMB和0.2ml/L浓HCl),10min;加入终止液(50μl/孔,含0.75g/EDTA·2Na和10.2ml/L浓H2SO4);酶标仪上450nm(参考630nm)处读OD值。结果见下表2。
表2 WT抗体及其突变体的活性数据
抗体浓度(ng/ml) | 5000 | 1000 | 500 | 250 | 125 | 0.00 |
WT | 0.712 | 0.731 | 0.351 | 0.152 | 0.52 | 0.090 |
突变1 | 0.952 | 0.825 | 0.625 | 0.364 | 0.204 | 0.025 |
(2)抗体及其突变体的亲和力检测
在突变1的基础上,对其他位点进行突变,各突变的序列见下表3。
表3与抗体亲和力有关的突变位点
亲和力分析
利用AMC传感器,将纯化出来的抗体用PBST稀释到10μg/mL,2019-nCoV N蛋白抗原用PBST进行梯度稀释:1.41μg/mL、0.70μg/mL、0.35μg/mL、0.18μg/mL、0.09μg/mL、0.04μg/mL。
运行流程:缓冲液1(PBST)中平衡60s,抗体溶液中固化抗体300s,缓冲液2(PBST)中孵育180s,抗原溶液中结合420s,缓冲液2中解离1200s,用10mM pH 1.69GLY溶液及缓冲液3进行传感器再生,输出数据。KD表示平衡解离常数即亲和力;kon表示结合速率;kdis表示解离速率。结果见下表4。
表4亲和力检测数据
KD(M) | kon(1/Ms) | kdis(1/s) | |
突变1-18 | 1.93E-10 | 3.41E+06 | 6.59E-04 |
以上所述仅为本发明的优选实施例而已,并不用于限制本发明,对于本领域的技术人员来说,本发明可以有各种更改和变化。凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。
本分案申请涉及的SEQ ID NO.15-18序列如下:
序列表
<110> 东莞市朋志生物科技有限公司
<120> 抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒
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50 55 60
Ile Arg Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Val Gln Ala
65 70 75 80
Glu Asp Leu Ala Leu Tyr Tyr Cys Gln Gln His Trp Ser Thr Pro Leu
85 90 95
Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg
100 105
<210> 12
<211> 119
<212> PRT
<213> 人工序列
<400> 12
Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu
1 5 10 15
Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Asp Tyr
20 25 30
Ala Met Asn Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met
35 40 45
Gly Trp Leu Asn Thr Tyr Ser Gly Glu Pro Thr Tyr Ala Glu Asp Phe
50 55 60
Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr
65 70 75 80
Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys
85 90 95
Ala Arg Thr Ala Ile Leu Arg Ser Tyr Phe Asp Tyr Trp Gly Gln Gly
100 105 110
Thr Thr Leu Thr Val Ser Ser
115
<210> 13
<211> 214
<212> PRT
<213> 人工序列
<400> 13
Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly
1 5 10 15
Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Glu Asp Leu Ser Thr Ala
20 25 30
Leu Ala Trp Cys Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile
35 40 45
Tyr Trp Gly Ser Thr Arg His Ser Gly Val Pro Asp Arg Phe Thr Gly
50 55 60
Ile Arg Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Val Gln Ala
65 70 75 80
Glu Asp Leu Ala Leu Tyr Tyr Cys Gln Gln His Trp Ser Thr Pro Leu
85 90 95
Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala
100 105 110
Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly
115 120 125
Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile
130 135 140
Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu
145 150 155 160
Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser
165 170 175
Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr
180 185 190
Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser
195 200 205
Phe Asn Arg Asn Glu Cys
210
<210> 14
<211> 443
<212> PRT
<213> 人工序列
<400> 14
Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu
1 5 10 15
Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Asp Tyr
20 25 30
Ala Met Asn Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met
35 40 45
Gly Trp Leu Asn Thr Tyr Ser Gly Glu Pro Thr Tyr Ala Glu Asp Phe
50 55 60
Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr
65 70 75 80
Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys
85 90 95
Ala Arg Thr Ala Ile Leu Arg Ser Tyr Phe Asp Tyr Trp Gly Gln Gly
100 105 110
Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr
115 120 125
Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu
130 135 140
Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp
145 150 155 160
Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser
180 185 190
Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser
195 200 205
Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys
210 215 220
Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro
225 230 235 240
Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr
245 250 255
Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser
260 265 270
Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg
275 280 285
Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile
290 295 300
Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn
305 310 315 320
Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys
325 330 335
Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu
340 345 350
Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe
355 360 365
Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala
370 375 380
Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr
385 390 395 400
Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly
405 410 415
Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His
420 425 430
Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys
435 440
<210> 15
<211> 119
<212> PRT
<213> 人工序列
<400> 15
Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu
1 5 10 15
Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr
20 25 30
Gly Met Asn Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met
35 40 45
Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe
50 55 60
Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr
65 70 75 80
Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys
85 90 95
Ala Arg Ser Ala Leu Leu Arg Ser Tyr Phe Asp Tyr Trp Gly Gln Gly
100 105 110
Thr Thr Leu Thr Val Ser Ser
115
<210> 16
<211> 108
<212> PRT
<213> 人工序列
<400> 16
Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly
1 5 10 15
Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala
20 25 30
Val Ala Trp Cys Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile
35 40 45
Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly
50 55 60
Ile Arg Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Val Gln Ala
65 70 75 80
Glu Asp Leu Ala Leu Tyr Tyr Cys Gln Gln His Tyr Ser Thr Pro Leu
85 90 95
Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg
100 105
<210> 17
<211> 443
<212> PRT
<213> 人工序列
<400> 17
Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu
1 5 10 15
Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr
20 25 30
Gly Met Asn Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met
35 40 45
Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe
50 55 60
Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr
65 70 75 80
Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys
85 90 95
Ala Arg Ser Ala Leu Leu Arg Ser Tyr Phe Asp Tyr Trp Gly Gln Gly
100 105 110
Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr
115 120 125
Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu
130 135 140
Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp
145 150 155 160
Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser
180 185 190
Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser
195 200 205
Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys
210 215 220
Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro
225 230 235 240
Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr
245 250 255
Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser
260 265 270
Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg
275 280 285
Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile
290 295 300
Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn
305 310 315 320
Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys
325 330 335
Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu
340 345 350
Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe
355 360 365
Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala
370 375 380
Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr
385 390 395 400
Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly
405 410 415
Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His
420 425 430
Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys
435 440
<210> 18
<211> 214
<212> PRT
<213> 人工序列
<400> 18
Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly
1 5 10 15
Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala
20 25 30
Val Ala Trp Cys Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile
35 40 45
Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly
50 55 60
Ile Arg Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Val Gln Ala
65 70 75 80
Glu Asp Leu Ala Leu Tyr Tyr Cys Gln Gln His Tyr Ser Thr Pro Leu
85 90 95
Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala
100 105 110
Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly
115 120 125
Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile
130 135 140
Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu
145 150 155 160
Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser
165 170 175
Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr
180 185 190
Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser
195 200 205
Phe Asn Arg Asn Glu Cys
210
Claims (10)
1.一种抗新型冠状病毒或其N蛋白的抗体或其功能性片段,其特征在于,所述抗体或其功能性片段包括如下互补决定区:
CDR-VH1:G-Y-T-F-T-N-Y-G-M-N;
CDR-VH2:W-I-N-T-Y-T-G-E-P-T-Y-A-D-D-F;
CDR-VH3:A-R-S-A-L-L-R-S-Y;
CDR-VL1:K-A-S-Q-D-V-S-T-A-V-A;
CDR-VL2:W-A-S-T-R-H-T;
CDR-VL3:Q-Q-H-Y-S-T-P-L;
和,序列依次与SEQ ID NO:1-4具有至少80%同源性的轻链骨架区FR1-L、FR2-L、FR3-L、FR4-L,和/或,序列依次与SEQ ID NO:5-8具有至少80%同源性的重链骨架区FR1-H、FR2-H、FR3-H、FR4-H。
2.根据权利要求1所述的抗体或其功能性片段,其特征在于,所述抗体还包含恒定区。
3.根据权利要求2所述的抗体或其功能性片段,其特征在于,所述恒定区选自IgG1、IgG2、IgG3、IgG4、IgA、IgM、IgE和IgD中的任意一者的恒定区。
4.根据权利要求2所述的抗体或其功能性片段,其特征在于,所述恒定区的种属来源为牛、马、猪、绵羊、山羊、大鼠、小鼠、狗、猫、兔、驴、鹿、貂、鸡、鸭、鹅或人。
5.根据权利要求2所述的抗体或其功能性片段,其特征在于,所述恒定区的轻链恒定区序列如SEQ ID NO:9所示,所述恒定区的重链恒定区序列如SEQ ID NO:10所示。
6.根据权利要求1至2任一所述的抗体或其功能性片段,其特征在于,所述功能性片段选自所述抗体的F(ab’)2、Fab’、Fab、Fv和scFv中的任意一种。
7.一种检测新型冠状病毒或其N蛋白的试剂或试剂盒,其特征在于,其包括如权利要求1-6任一项所述的抗体或其功能性片段。
8.一种载体,其特征在于,其含有编码权利要求1-6任一项所述的抗体或其功能性片段的核酸片段。
9.一种重组细胞,其特征在于,其含有权利要求8所述的载体。
10.一种制备权利要求1-6任一项所述的抗体或其功能性片段的方法,其特征在于,其包括:培养权利要求9所述的重组细胞,从培养产物中分离纯化得到所述抗体或其功能性片段。
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CN112239500B (zh) * | 2020-10-29 | 2022-01-07 | 东莞市朋志生物科技有限公司 | 抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒 |
CN116769042A (zh) | 2021-06-17 | 2023-09-19 | 东莞市朋志生物科技有限公司 | 嵌合的免疫球蛋白 |
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CN115112879A (zh) * | 2022-07-01 | 2022-09-27 | 重庆艾生斯生物工程有限公司 | 一种结合物及其在免疫检测中的应用 |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111518202A (zh) * | 2020-05-27 | 2020-08-11 | 江苏省疾病预防控制中心(江苏省公共卫生研究院) | 新型冠状病毒抗体和新型冠状病毒抗体的elisa检测试剂盒 |
CN111778218A (zh) * | 2020-06-04 | 2020-10-16 | 山东宽和正生物医药有限公司 | 噬菌体展示抗体库及基于其淘筛获得的针对新冠病毒SARS-CoV-2的单克隆抗体 |
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CN111273003A (zh) * | 2020-03-05 | 2020-06-12 | 广州万孚生物技术股份有限公司 | 2019-nCoV新型冠状病毒快速检测免疫层析试纸条 |
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CN111366735B (zh) * | 2020-03-20 | 2021-07-13 | 广州市康润生物科技有限公司 | 新型冠状病毒更早期筛查方法 |
CN111257555A (zh) * | 2020-04-03 | 2020-06-09 | 安徽省疾病预防控制中心(省健康教育所) | 新冠病毒核酸重组酶介导等温扩增侧向层析胶体金快速检测方法及试纸条 |
CN111551743B (zh) * | 2020-05-08 | 2021-02-05 | 北京贝尔生物工程股份有限公司 | 一种快速准确检测新型冠状病毒IgM抗体的试剂盒及其制备方法 |
CN111620946B (zh) * | 2020-05-09 | 2020-12-22 | 江苏省疾病预防控制中心(江苏省公共卫生研究院) | 分离的新型冠状病毒单克隆抗体或其抗原结合部分 |
CN111518203B (zh) * | 2020-05-27 | 2021-10-19 | 江苏省疾病预防控制中心(江苏省公共卫生研究院) | 用于新型冠状病毒检测的试剂盒 |
CN111560070B (zh) * | 2020-05-27 | 2021-10-01 | 江苏省疾病预防控制中心(江苏省公共卫生研究院) | 针对新型冠状病毒np蛋白的抗体及其检测应用 |
CN111748033B (zh) * | 2020-05-27 | 2021-02-02 | 江苏省疾病预防控制中心(江苏省公共卫生研究院) | 一种与新型冠状病毒np蛋白结合的分离抗体、包含其的检测试剂盒 |
CN111690060A (zh) * | 2020-07-06 | 2020-09-22 | 深圳市亚辉龙生物科技股份有限公司 | 能够特异性识别RBD蛋白的IgA抗体以及试剂盒 |
CN112239500B (zh) * | 2020-10-29 | 2022-01-07 | 东莞市朋志生物科技有限公司 | 抗新型冠状病毒的抗体和检测新型冠状病毒的试剂盒 |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111518202A (zh) * | 2020-05-27 | 2020-08-11 | 江苏省疾病预防控制中心(江苏省公共卫生研究院) | 新型冠状病毒抗体和新型冠状病毒抗体的elisa检测试剂盒 |
CN111778218A (zh) * | 2020-06-04 | 2020-10-16 | 山东宽和正生物医药有限公司 | 噬菌体展示抗体库及基于其淘筛获得的针对新冠病毒SARS-CoV-2的单克隆抗体 |
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