CN114409483A - Compound biological fertilizer and preparation method and application thereof - Google Patents

Compound biological fertilizer and preparation method and application thereof Download PDF

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Publication number
CN114409483A
CN114409483A CN202210094015.3A CN202210094015A CN114409483A CN 114409483 A CN114409483 A CN 114409483A CN 202210094015 A CN202210094015 A CN 202210094015A CN 114409483 A CN114409483 A CN 114409483A
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China
Prior art keywords
trichoderma
compound
powder
trichoderma harzianum
asperellum
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Chinese (zh)
Inventor
刘振
武俊刚
李响
夏威
杨洪波
秦建
刘庆鹏
张敬鑫
李文
张荣沭
吴建慧
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Northeast Forestry University
Longjian Road and Bridge Co Ltd
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Northeast Forestry University
Longjian Road and Bridge Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants

Abstract

A compound biological fertilizer and a preparation method and application thereof relate to a compound biological fertilizer and a preparation method and application thereof. The invention aims to solve the problems that the environmental adaptability of strains in the existing biological compound fertilizer is poor, the application amount is large, the strains are required to be applied continuously every year, the production cost is high, and the problems that the flowering phase of impatiens is short and powdery mildew is easy to occur by using the existing biological compound fertilizer cannot be solved. The compound biofertilizer comprises trichoderma asperellum, trichoderma harzianum and talcum powder, and the preparation method comprises the steps of preparing bacterial powder and mixing to obtain the compound biofertilizer. The compound biological fertilizer is used as a special fertilizer for impatiens balsamina. The advantages are that: has multiple effects of promoting growth of biological fertilizer, soil conditioner, fungicide biological pesticide and the like; without the need for annual uninterrupted application. The fertilizer is used as a fertilizer special for impatiens balsamina, can promote growth of impatiens balsamina to enable the impatiens balsamina to bloom in advance, prolong flowering phase and improve powdery mildew resistance.

Description

Compound biological fertilizer and preparation method and application thereof
Technical Field
The invention relates to a compound biofertilizer and a preparation method and application thereof.
Background
The population of China is large, and the food yield can directly influence the life stability of people; therefore, as a large country for grain production in China, the utilization of soil is nearly rigorous, in order to improve the annual average yield, a planting mode of more than one crop in a year is adopted, and a large amount of chemical fertilizer is used, so that the fertility of the soil is reduced year by year, soil hardening is caused, the quality of agricultural products is reduced, the ecology is seriously damaged, and the environment is polluted; in order to save time and labor, a large amount of pesticides are used in the planting process, the ecological balance is destroyed while weeding and deinsectization are achieved, river water is seriously polluted, the river bed is dried, and fishes and shrimps are heavily died. And the quality of agricultural products is seriously reduced, and the phenomena of not fragrant grains, not tender vegetables and not sweet fruits occur. In order to achieve the current sustainable development, China advocates developing high-efficiency biological organic fertilizer, reduces the using amount of chemical fertilizer, increases the organic matters of soil and realizes the aims of high yield, high quality and high efficiency; the biological compound fertilizer becomes an indispensable raw material for improving saline-alkali soil and degrading grassland and is also an optimal fertilizer source for promoting the continuous and reasonable utilization of soil. However, the environmental adaptability of the strains in the existing biological compound fertilizer is poor, and in order to achieve the purpose of improving soil, the application amount is large, the strains are required to be applied continuously every year, so that the production cost is greatly increased, and the strains cannot be well popularized and applied.
Disclosure of Invention
The invention aims to solve the problems that the environmental adaptability of strains in the existing biological compound fertilizer is poor, the application amount is large, the strains are required to be applied continuously every year, the production cost is high, and the problems that the flowering phase of impatiens is short and powdery mildew is easy to occur cannot be solved by using the existing biological compound fertilizer; and provides a compound biological fertilizer and a preparation method and application thereof.
A compound biofertilizer comprising Trichoderma asperellum, Trichoderma harzianum and pulvis Talci; the effective viable count in the compound biological fertilizer is more than or equal to 0.5 multiplied by 109CFU/g, wherein the effective viable count of Trichoderma asperellum is 0.2 × 109CFU/g～0.5×109CFU/g, effective viable count of Trichoderma harzianum of 0.3 × 109CFU/g～0.8×109CFU/g, wherein the rate of mixed bacteria in the compound biological fertilizer is lower than 1%; the pH value of the compound biological fertilizer is 6.0-7.5.
A preparation method of a compound biofertilizer is specifically completed according to the following steps:
firstly, preparing bacterial powder:
(1) and culturing a seed solution: respectively inoculating trichoderma asperellum and trichoderma harzianum into a seed culture medium for culture for 6-8 hours to respectively obtain trichoderma asperellum seed liquid and trichoderma harzianum seed liquid;
(2) and inoculation and fermentation: respectively inoculating the trichoderma asperellum strain seed liquid and the trichoderma harzianum seed liquid into a fermentation culture medium, and respectively stirring and culturing for 8-12 h under the conditions of temperature of 30-33 ℃ and oxygen introduction to respectively obtain trichoderma asperellum liquid and trichoderma harzianum liquid;
(3) transferring the trichoderma asperellum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (30-40), culturing for 10-14 h under the condition that the temperature is 30-36 ℃, drying at low temperature and crushing to obtain trichoderma asperellum powder; transferring the trichoderma harzianum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (40-50), culturing for 10-14 h under the condition of the temperature of 30-36 ℃, and drying and crushing at low temperature to obtain trichoderma harzianum powder.
Secondly, mixing: uniformly mixing the trichoderma asperellum powder, the trichoderma harzianum powder and the talcum powder to obtain a compound biological fertilizer; the effective viable count in the compound biological fertilizer is more than or equal to 0.5 multiplied by 109CFU/g, wherein the effective viable count of Trichoderma asperellum is 0.2 × 109CFU/g～0.5×109CFU/g, effective viable count of Trichoderma harzianum of 0.3 × 109CFU/g～0.8×109CFU/g, wherein the rate of mixed bacteria in the compound biological fertilizer is lower than 1%; the pH value of the compound biological fertilizer is 6.0-7.5.
An application of a compound biological fertilizer, which is used as a fertilizer special for impatiens balsamina.
The invention has the advantages that:
the compound biofertilizer selects trichoderma asperellum and trichoderma harzianum as strains for improving soil nutrients, the trichoderma harzianum and trichoderma harzianum have strong vitality and high propagation speed, are suitable for various soil types, and are basically not influenced by the external environment once the trichoderma harzianum and trichoderma harzianum establish symbiotic relationship with plants in plant root systems; moreover, the combined use of trichoderma harzianum and trichoderma asperellum can effectively prevent and treat soil-borne diseases of crop roots caused by pythium, rhizoctonia solani, fusarium, sclerotinia sclerotiorum and the like; the action mechanism comprises space competition, antagonism, bacteriolysis, secretion of antibiotics and enzymes, crop growth stimulation and immune response induction.
Secondly, the compound biological fertilizer is applied as base fertilizer according to the application amount of 800 kg/mu in the first year, and is applied as base fertilizer according to the application amount of 500 kg/mu in the second year, wherein the compound biological fertilizer is applied for 1 time every three years from the third year, and the compound biological fertilizer is applied as base fertilizer according to the application amount of 300 kg/mu every time, so that the problem of high production cost caused by annual uninterrupted application of the existing biological compound fertilizer is solved.
Thirdly, the compound biofertilizer is used as a fertilizer special for impatiens balsamina, the compound biofertilizer and common flower soil are mixed according to the mass ratio of 1:1 to obtain soil special for impatiens balsamina, and the soil special for impatiens balsamina is used for planting impatiens balsamina, so that the growth of impatiens and peanuts can be promoted, the impatiens can bloom in advance (1-3 days ahead), the flowering phase of the impatiens is prolonged (5-12 days are prolonged), and the powdery mildew resistance of the impatiens is improved.
And fourthly, the compound biofertilizer has multiple effects of promoting growth of the biofertilizer, improving soil, killing fungi and biological pesticides and the like. Can stimulate seed germination, root elongation, plant growth and early flowering and fructification; and has the capability of decomposing organic matters and heavy metal pollution of soil, removing salinization of soil and the like.
And fifthly, the compound biological fertilizer is safe and reliable, and has no adverse effect on human, livestock and ecological environment.
Drawings
FIG. 1 is a water content-time bar graph, in which A represents a water content-time bar graph of a control group, B represents a water content-time bar graph of an experimental group 1, C represents a water content-time bar graph of an experimental group 2, and D represents a water content-time bar graph of an experimental group 3;
FIG. 2 is a pH-time bar graph in which A represents a pH-time bar graph of a control group, B represents a pH-time bar graph of an experimental group 1, C represents a pH-time bar graph of an experimental group 2, and D represents a pH-time bar graph of an experimental group 3;
FIG. 3 is a histogram of organic matter versus time in which A is a histogram of organic matter versus time in the control group, B is a histogram of organic matter versus time in the experimental group 1, C is a histogram of organic matter versus time in the experimental group 2, and D is a histogram of organic matter versus time in the experimental group 3;
FIG. 4 is a dry weight-time bar graph, in which A represents a dry weight-time bar graph of a control group of Artemisia annua seedlings, in which B represents a dry weight-time bar graph of an experiment group 1 Artemisia annua seedlings, in which C represents a dry weight-time bar graph of an experiment group 2 Artemisia annua seedlings, and in which D represents a dry weight-time bar graph of an experiment group 3 Artemisia annua seedlings.
Detailed Description
The first embodiment is as follows: the embodiment is a compound biofertilizer which comprises trichoderma asperellum, trichoderma harzianum and talcum powder; the effective viable count in the compound biological fertilizer is more than or equal to 0.5 multiplied by 109CFU/g, wherein the effective viable count of Trichoderma asperellum is 0.2 × 109CFU/g～0.5×109CFU/g, effective viable count of Trichoderma harzianum of 0.3 × 109CFU/g～0.8×109CFU/g, wherein the rate of mixed bacteria in the compound biological fertilizer is lower than 1%; the pH value of the compound biological fertilizer is 6.0-7.5.
The compound biofertilizer of the embodiment is applied as a base fertilizer at an application rate of 800 kg/mu in the first year, is applied as a base fertilizer at an application rate of 500 kg/mu in the second year, is applied 1 time every three years from the third year, and is applied as a base fertilizer at an application rate of 300 kg/mu each time.
The second embodiment is as follows: the present embodiment differs from the first embodiment in that: the compound biofertilizer is prepared by mixing trichoderma asperellum powder, trichoderma harzianum powder and talcum powder. The rest is the same as the first embodiment.
The third concrete implementation mode: the present embodiment differs from the first or second embodiment in that: the trichoderma asperellum is one or more of trichoderma asperellum ACCC30536, trichoderma asperellum ACCC32492, trichoderma asperellum ACCC31650 and trichoderma asperellum CGMCC 11653; and the preservation number of the trichoderma asperellum ACCC30536 is as follows: ACCC 30536; the preservation number of the trichoderma asperellum ACCC32492 is as follows: ACCC 32492; the preservation number of the trichoderma asperellum ACCC31650 is as follows: ACCC 31650; the preservation number of the trichoderma asperellum CGMCC11653 is as follows: CGMCC 11653. The others are the same as in the first or second embodiment.
The fourth concrete implementation mode: the present embodiment differs from the first or second embodiment in that: the Trichoderma harzianum is one or more of Trichoderma harzianum CGMCC11120, Trichoderma harzianum CGMCC11121 and Trichoderma harzianum CGMCC 11122; and the preservation number of the trichoderma harzianum CGMCC11120 is as follows: CGMCC 11120; the preservation number of the trichoderma harzianum CGMCC11121 is as follows: CGMCC 11121; the preservation number of the trichoderma harzianum CGMCC11122 is as follows: CGMCC 11122. The others are the same as in the first or second embodiment.
The fifth concrete implementation mode: the second to fourth embodiments are different from the first to fourth embodiments in that: the trichoderma asperellum powder and trichoderma harzianum powder are prepared according to the following steps:
(1) and culturing a seed solution: respectively inoculating trichoderma asperellum and trichoderma harzianum into a seed culture medium for culture for 6-8 hours to respectively obtain trichoderma asperellum seed liquid and trichoderma harzianum seed liquid;
(2) and inoculation and fermentation: respectively inoculating the trichoderma asperellum strain seed liquid and the trichoderma harzianum seed liquid into a fermentation culture medium, and respectively stirring and culturing for 8-12 h under the conditions of temperature of 30-33 ℃ and oxygen introduction to respectively obtain trichoderma asperellum liquid and trichoderma harzianum liquid;
(3) transferring the trichoderma asperellum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (30-40), culturing for 10-14 h under the condition that the temperature is 30-36 ℃, drying at low temperature and crushing to obtain trichoderma asperellum powder; transferring the trichoderma harzianum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (40-50), culturing for 10-14 h under the condition of the temperature of 30-36 ℃, and drying and crushing at low temperature to obtain trichoderma harzianum powder.
The other points are the same as those in the second to fourth embodiments.
In the step (3) of the embodiment, the edible fungi cultivation waste is cultivation mushroom dregs which are not polluted by mixed fungi, specifically, the cultivation mushroom dregs are agaric cultivation mushroom dregs, needle mushroom cultivation mushroom dregs, pleurotus eryngii cultivation mushroom dregs or pleurotus nebrodensis cultivation mushroom dregs.
The sixth specific implementation mode: the present embodiment is different from the fifth embodiment in that: the seed culture medium in the step (1) is a beef extract peptone culture medium. The rest is the same as the fifth embodiment.
The seventh embodiment: the present embodiment is different from the fifth or sixth embodiment in that: the fermentation medium in the step (1) comprises the following raw materials: each liter of fermentation medium contains 5-15 g of sucrose,5-12 g of lactose, 1-5 g of maltose, 1-5 g of soluble starch, 5-15 g of peptone, 2-9 g of yeast extract, 1-5 g of urea, 3-8 g of NaCl, and 0.4-1.0 g K2SO4、0.5～1.5g K2HPO4、2～3.5g MgSO4·7H2O、0.8～1.5g CaCl2、4～6.5g CaCO3And the balance water. The other is the same as the fifth or sixth embodiment.
The specific implementation mode is eight: the embodiment is a preparation method of a compound biofertilizer, which is specifically completed by the following steps:
firstly, preparing bacterial powder:
(1) and culturing a seed solution: respectively inoculating trichoderma asperellum and trichoderma harzianum into a seed culture medium for culture for 6-8 hours to respectively obtain trichoderma asperellum seed liquid and trichoderma harzianum seed liquid;
(2) and inoculation and fermentation: respectively inoculating the trichoderma asperellum strain seed liquid and the trichoderma harzianum seed liquid into a fermentation culture medium, and respectively stirring and culturing for 8-12 h under the conditions of temperature of 30-33 ℃ and oxygen introduction to respectively obtain trichoderma asperellum liquid and trichoderma harzianum liquid;
(3) transferring the trichoderma asperellum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (30-40), culturing for 10-14 h under the condition that the temperature is 30-36 ℃, drying at low temperature and crushing to obtain trichoderma asperellum powder; transferring the trichoderma harzianum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (40-50), culturing for 10-14 h under the condition of the temperature of 30-36 ℃, and drying and crushing at low temperature to obtain trichoderma harzianum powder.
Secondly, mixing: uniformly mixing the trichoderma asperellum powder, the trichoderma harzianum powder and the talcum powder to obtain a compound biological fertilizer; the effective viable count in the compound biological fertilizer is more than or equal to 0.5 multiplied by 109CFU/g, wherein the effective viable count of Trichoderma asperellum is 0.2 × 109CFU/g～0.5×109CFU/g, effective viable count of Trichoderma harzianum of 0.3 × 109CFU/g～0.8×109CFU/g, wherein the rate of mixed bacteria in the compound biological fertilizer is lower than 1%; the pH value of the compound biological fertilizer is 6.0-7.5.
In the first step (3) of the present embodiment, the waste edible fungus cultivation material is cultivation mushroom dregs which are not polluted by mixed mushrooms, specifically, agaric cultivation mushroom dregs, flammulina velutipes cultivation mushroom dregs, pleurotus eryngii cultivation mushroom dregs or pleurotus nebrodensis cultivation mushroom dregs.
The specific implementation method nine: the present embodiment is different from the eighth embodiment in that: the seed culture medium in the step one (1) is a beef extract peptone culture medium. The rest is the same as the embodiment eight.
The detailed implementation mode is ten: the eighth embodiment is different from the ninth embodiment in that: the fermentation medium in the step one (1) comprises the following raw materials: each liter of fermentation medium contains 5-15 g of sucrose, 5-12 g of lactose, 1-5 g of maltose, 1-5 g of soluble starch, 5-15 g of peptone, 2-9 g of yeast extract, 1-5 g of urea, 3-8 g of NaCl, 0.4-1.0 g K2SO4、0.5～1.5gK2HPO4、2～3.5g MgSO4·7H2O、0.8～1.5g CaCl2、4～6.5g CaCO3And the balance water. The others are the same as the embodiments eight or nine.
The concrete implementation mode eleven: the eighth to tenth embodiments are different from the first to eighth embodiments in that: and in the second step, the trichoderma asperellum bacteria powder, the trichoderma harzianum bacteria powder and the talcum powder are mixed for 1 to 1.5 hours in a vortex mode by adopting a vortex mixer. The rest is the same as the embodiment eight. The others are the same as the embodiments eight to ten.
The specific implementation mode twelve: the embodiment is an application of the compound biological fertilizer, and the compound biological fertilizer is used as a special fertilizer for impatiens balsamina.
In the embodiment, the compound biological fertilizer is mixed with common flower soil according to the mass ratio of 1:1 to obtain the special soil for impatiens balsamina, and the special soil for impatiens balsamina is used for planting impatiens balsamina according to a conventional method.
The invention is not limited to the above embodiments, and one or a combination of several embodiments may also achieve the object of the invention.
The following tests are adopted to verify the effect of the invention:
example 1: a preparation method of a compound biofertilizer is specifically completed according to the following steps:
firstly, preparing bacterial powder:
(1) and culturing a seed solution: respectively inoculating trichoderma asperellum and trichoderma harzianum into a seed culture medium for culture for 8h to respectively obtain trichoderma asperellum seed liquid and trichoderma harzianum seed liquid;
(2) and inoculation and fermentation: respectively inoculating the trichoderma asperellum strain seed liquid and the trichoderma harzianum seed liquid into a fermentation culture medium, and respectively stirring and culturing for 10 hours at the temperature of 33 ℃ under the condition of introducing oxygen to respectively obtain trichoderma asperellum liquid and trichoderma harzianum liquid;
(3) transferring the trichoderma asperellum liquid and the edible fungus cultivation waste into a solid culture tank according to the mass ratio of 1:35, culturing for 12 hours at the temperature of 35 ℃, drying and crushing at low temperature to obtain trichoderma asperellum powder; transferring the trichoderma harzianum liquid and the edible fungus cultivation waste into a solid culture tank according to the mass ratio of 1:45, culturing for 12h under the condition of the temperature of 35 ℃, and drying and crushing at low temperature to obtain trichoderma harzianum powder.
Secondly, mixing: uniformly mixing the trichoderma asperellum powder, the trichoderma harzianum powder and the talcum powder to obtain a compound biological fertilizer; the effective viable count of the compound biological fertilizer is about 1.1 multiplied by 109CFU/g, wherein the effective viable count of Trichoderma asperellum is 0.41 × 109CFU/g, effective viable count of Trichoderma harzianum of 0.69 × 109CFU/g, wherein the rate of mixed bacteria in the compound biological fertilizer is lower than 1%; the pH value of the compound biological fertilizer is 6.6.
In the first step (1) of this example, the seed culture medium is beef extract peptone culture medium.
The raw materials of the fermentation medium in step one (1) of this example are: each liter of fermentation medium contains 10g of sucrose, 8g of lactose, 2g of maltose, 3g of soluble starch, 10g of peptone, 5g of yeast extract, 3g of urea, 6g of NaCl, and 0.6g K2SO4、1.0g K2HPO4、3g MgSO4·7H2O、1.1g CaCl2、5.1g CaCO3And the balance water.
In the first step (3) of the present embodiment, the waste edible fungus cultivation material is Pleurotus nebrodensis cultivation residue which is not contaminated by miscellaneous fungi.
In the second step of this example, a vortex mixer was used to vortex mix the Trichoderma asperellum bacteria powder, Trichoderma harzianum bacteria powder, and pulvis Talci for 1 h.
The method comprises the following steps of taking sweet wormwood seedlings as application objects, and normally applying common fertilizers, namely, the fertilizing amount of a nitrogen fertilizer (urea) is 0.5 kg/plant, the fertilizing amount of a phosphate fertilizer (calcium phosphate) is 0.5 kg/plant, the fertilizing amount of a potassium fertilizer (potassium chloride) is 0.5 kg/plant, and the fertilizing amount of biological humic acid is 1 kg/plant; the control group is not applied with the compound biofertilizer, the fertilizing amount of the compound biofertilizer of the experimental group is 0.3 kg/plant, the experimental group is provided with three parallel experiments which are respectively an experimental group 1, an experimental group 2 and an experimental group 3, the experiment group is cultured for 60D, the change of the water content, the pH value and the organic matter in the soil when the soil is cultured for 0D, the change of the water content, the pH value and the organic matter in the soil when the soil is cultured for 15D, the change of the organic matter in the soil when the soil is cultured for 30D, the soil is cultured for 40D, the soil is cultured for 60D, and the dry weight of the sweet wormwood seedlings when the soil is cultured for 0D, the dry weight of the sweet wormwood seedlings when the soil is cultured for 40D, the dry weight of the sweet wormwood seedlings is recorded respectively, as shown in figures 1 to 4, wherein, the figure 1 is a water content-time bar graph, A shows a water content-time bar graph of the control group, a graph shows a water content-time bar graph of the experimental group 1, a graph shows a water content-time bar graph of the experimental group 2, and a graph shows a graph D shows a graph of the experiment group 3; FIG. 2 is a pH-time bar graph in which A represents a pH-time bar graph of a control group, B represents a pH-time bar graph of an experimental group 1, C represents a pH-time bar graph of an experimental group 2, and D represents a pH-time bar graph of an experimental group 3; FIG. 3 is a histogram of organic matter versus time in which A is a histogram of organic matter versus time in the control group, B is a histogram of organic matter versus time in the experimental group 1, C is a histogram of organic matter versus time in the experimental group 2, and D is a histogram of organic matter versus time in the experimental group 3; FIG. 4 is a dry weight-time bar graph in which A represents a dry weight-time bar graph of a control group of Artemisia annua seedlings, B represents a dry weight-time bar graph of an experiment group 1 Artemisia annua seedlings, C represents a dry weight-time bar graph of an experiment group 2 Artemisia annua seedlings, and D represents a dry weight-time bar graph of an experiment group 3 Artemisia annua seedlings; as can be seen from fig. 1, the water content of the soil in the experimental group is significantly increased compared with that in the initial (0d) within the range of 0-15 d of the cultivation time, and the water content of the soil in the experimental group is significantly higher than that of the soil in the control group within the range of 16-45 d of the cultivation time, which indicates that the compound biofertilizer prepared by the invention can adsorb free water molecules in the air into the soil, and further increase the water content of the soil; as can be seen from FIG. 4, the growing blocks of the seedlings of Artemisia annua in the experimental group consume a large amount of water during the growing process, so that the water content in the soil of the experimental group is slightly lower than that in the soil of the control group when the cultivation time is in the range of 46-60 days. As can be seen from FIG. 2, the pH value in the soil of the experimental group is significantly lower than that in the soil of the control group within the range of 0-45 d of the cultivation time, which shows that the pH value of the soil can be significantly reduced by the composite biofertilizer prepared by the invention, but the pH value of the soil can also be affected by the growth of plants, and the pH value in the soil of the experimental group varies to different extents within the range of 46-60 d of the cultivation time due to the growth of the seedlings of Artemisia annua of the experimental group. As can be seen from the graph 3, the organic matter content in the experimental group soil is obviously higher than that in the control group soil within the range of 0-60 d of the culture time, which shows that the organic matter content of the soil can be obviously improved by the composite biological fertilizer prepared by the invention; as can be seen from fig. 4, the experimental group showed a more massive growth of seedlings of artemisia annua; in conclusion, the compound biological fertilizer prepared by the invention can improve the soil fertility and promote the plant growth (sweet wormwood seedling).
Example 2: the application of the compound biological fertilizer is that the compound biological fertilizer is used as a fertilizer special for impatiens balsamina; and (3) mixing the compound biological fertilizer with common flower soil according to the mass ratio of 1:1 to obtain the special soil for the impatiens balsamina, and planting the impatiens balsamina by utilizing the special soil for the impatiens balsamina according to a conventional method.
Comparative example 1: control group without compound biofertilizer addition: the impatiens balsamina is planted by using common flower soil according to a conventional method.
The growth of the impatiens balsamina in example 2 and comparative example 1 was observed and recorded as shown in table 1; as can be seen from Table 1, after the compound biofertilizer is used as a fertilizer special for impatiens balsamina, the plant height, the ground diameter and the crown width of the impatiens balsamina are obviously improved, and the number of lateral branches and the number of leaves are obviously increased.
TABLE 1
Figure BDA0003490164850000071
Note: different lower case letters indicate a significance level of P < 0.05.
The blooming phenological conditions of impatiens balsamina in example 2 and comparative example 1 were observed and recorded as shown in table 2; as can be seen from Table 2, after the compound bio-fertilizer is used as the fertilizer special for impatiens balsamina, the initial flowering phase, full flowering phase, current flowering phase and current fruit phase of impatiens balsamina are all advanced, and the final flowering phase is delayed, so that the flowering phase of impatiens balsamina is prolonged by 10 days.
TABLE 2
Figure BDA0003490164850000072
The flowering amount of impatiens balsamina in example 2 and comparative example 1 was observed and recorded as shown in table 3; as can be seen from Table 3, the average flowering rate of each plant of impatiens balsamina in the full-bloom period is significantly increased by using the compound biofertilizer as the fertilizer special for impatiens balsamina.
TABLE 3
Figure BDA0003490164850000073
Figure BDA0003490164850000081
The onset of powdery mildew of impatiens balsamina in example 2 and comparative example 1 was observed and recorded as shown in table 4; as can be seen from table 4, the diseased plant rate of impatiens balsamina is significantly reduced when the compound biofertilizer is used as a fertilizer special for impatiens balsamina.
TABLE 4
Figure BDA0003490164850000082
Note: different lower case letters indicate a significance level of P < 0.05.

Claims (10)

1. The compound biofertilizer is characterized in that the compound biofertilizer comprises trichoderma asperellum, trichoderma harzianum and talcum powder; the effective viable count in the compound biological fertilizer is more than or equal to 0.5 multiplied by 109CFU/g, wherein the effective viable count of Trichoderma asperellum is 0.2 × 109CFU/g～0.5×109CFU/g, effective viable count of Trichoderma harzianum of 0.3 × 109CFU/g～0.8×109CFU/g, wherein the rate of mixed bacteria in the compound biological fertilizer is lower than 1%; the pH value of the compound biological fertilizer is 6.0-7.5.
2. The compound biofertilizer of claim 1, wherein the compound biofertilizer is prepared by mixing trichoderma asperellum powder, trichoderma harzianum powder and talc powder.
3. The compound biofertilizer of claim 1 or 2, wherein said trichoderma asperellum is one or more selected from trichoderma asperellum ACCC30536, trichoderma asperellum ACCC32492, trichoderma asperellum ACCC31650 and trichoderma asperellum CGMCC 11653; and the preservation number of the trichoderma asperellum ACCC30536 is as follows: ACCC 30536; the preservation number of the trichoderma asperellum ACCC32492 is as follows: ACCC 32492; the preservation number of the trichoderma asperellum ACCC31650 is as follows: ACCC 31650; the preservation number of the trichoderma asperellum CGMCC11653 is as follows: CGMCC 11653.
4. The compound biofertilizer of claim 1 or 2, wherein said trichoderma harzianum is one or more of trichoderma harzianum CGMCC11120, trichoderma harzianum CGMCC11121 and trichoderma harzianum CGMCC 11122; and the preservation number of the trichoderma harzianum CGMCC11120 is as follows: CGMCC 11120; the preservation number of the trichoderma harzianum CGMCC11121 is as follows: CGMCC 11121; the preservation number of the trichoderma harzianum CGMCC11122 is as follows: CGMCC 11122.
5. The compound biofertilizer of claim 2, wherein said trichoderma asperellum powder and trichoderma harzianum powder are prepared by the following steps:
(1) and culturing a seed solution: respectively inoculating trichoderma asperellum and trichoderma harzianum into a seed culture medium for culture for 6-8 hours to respectively obtain trichoderma asperellum seed liquid and trichoderma harzianum seed liquid;
(2) and inoculation and fermentation: respectively inoculating the trichoderma asperellum strain seed liquid and the trichoderma harzianum seed liquid into a fermentation culture medium, and respectively stirring and culturing for 8-12 h under the conditions of temperature of 30-33 ℃ and oxygen introduction to respectively obtain trichoderma asperellum liquid and trichoderma harzianum liquid;
(3) transferring the trichoderma asperellum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (30-40), culturing for 10-14 h under the condition that the temperature is 30-36 ℃, drying at low temperature and crushing to obtain trichoderma asperellum powder; transferring the trichoderma harzianum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (40-50), culturing for 10-14 h under the condition of the temperature of 30-36 ℃, and drying and crushing at low temperature to obtain trichoderma harzianum powder.
6. The compound biofertilizer of claim 5, wherein said seed medium in step (1) is beef extract peptone medium.
7. The compound biofertilizer of claim 5, wherein the raw material composition of said fermentation medium in step (1) is: each liter of fermentation medium contains 5-15 g of sucrose, 5-12 g of lactose, 1-5 g of maltose, 1-5 g of soluble starch, 5-15 g of peptone, 2-9 g of yeast extract, 1-5 g of urea, 3-8 g of NaCl, 0.4-1.0 g K2SO4、0.5～1.5g K2HPO4、2～3.5g MgSO4·7H2O、0.8～1.5g CaCl2、4～6.5g CaCO3And the balance water.
8. The method for preparing a compound biofertilizer according to claim 1, characterized in that it is carried out by the following steps:
firstly, preparing bacterial powder:
(1) and culturing a seed solution: respectively inoculating trichoderma asperellum and trichoderma harzianum into a seed culture medium for culture for 6-8 hours to respectively obtain trichoderma asperellum seed liquid and trichoderma harzianum seed liquid;
(2) and inoculation and fermentation: respectively inoculating the trichoderma asperellum strain seed liquid and the trichoderma harzianum seed liquid into a fermentation culture medium, and respectively stirring and culturing for 8-12 h under the conditions of temperature of 30-33 ℃ and oxygen introduction to respectively obtain trichoderma asperellum liquid and trichoderma harzianum liquid;
(3) transferring the trichoderma asperellum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (30-40), culturing for 10-14 h under the condition that the temperature is 30-36 ℃, drying at low temperature and crushing to obtain trichoderma asperellum powder; transferring the trichoderma harzianum liquid and the edible fungus cultivation waste material into a solid culture tank according to the mass ratio of 1 (40-50), culturing for 10-14 h under the condition of the temperature of 30-36 ℃, and drying and crushing at low temperature to obtain trichoderma harzianum powder.
Secondly, mixing: uniformly mixing the trichoderma asperellum powder, the trichoderma harzianum powder and the talcum powder to obtain a compound biological fertilizer; the effective viable count in the compound biological fertilizer is more than or equal to 0.5 multiplied by 109CFU/g, wherein the effective viable count of Trichoderma asperellum is 0.2 × 109CFU/g～0.5×109CFU/g, effective viable count of Trichoderma harzianum of 0.3 × 109CFU/g～0.8×109CFU/g, wherein the rate of mixed bacteria in the compound biological fertilizer is lower than 1%; the pH value of the compound biological fertilizer is 6.0-7.5.
9. The method for preparing a compound biofertilizer according to claim 8, characterized in that in step two, trichoderma asperellum powder, trichoderma harzianum powder and talcum powder are mixed by vortex for 1h to 1.5h by using a vortex mixer.
10. The application of the compound biofertilizer is characterized in that the compound biofertilizer is used as a fertilizer special for impatiens balsamina.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104312930A (en) * 2014-10-27 2015-01-28 中国农业科学院油料作物研究所 Method for preparing spraying type complex microbial inoculant for decomposing rape stalks and sclerotia
CN106083313A (en) * 2016-06-08 2016-11-09 杨子正 A kind of composite microbiological fertilizer and preparation method thereof
WO2017042833A1 (en) * 2015-09-11 2017-03-16 Zydex Industries Pvt. Ltd. Bio-fertilizer composition
CN109517744A (en) * 2019-01-10 2019-03-26 海南大学 The composite spore powder and its application of Trichoderma mixed cooperative solid fermentation preparation
CN111807893A (en) * 2020-06-23 2020-10-23 秦皇岛禾苗生物技术有限公司 Special continuous cropping resistant biological bacterial fertilizer for cucumbers

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104312930A (en) * 2014-10-27 2015-01-28 中国农业科学院油料作物研究所 Method for preparing spraying type complex microbial inoculant for decomposing rape stalks and sclerotia
WO2017042833A1 (en) * 2015-09-11 2017-03-16 Zydex Industries Pvt. Ltd. Bio-fertilizer composition
CN106083313A (en) * 2016-06-08 2016-11-09 杨子正 A kind of composite microbiological fertilizer and preparation method thereof
CN109517744A (en) * 2019-01-10 2019-03-26 海南大学 The composite spore powder and its application of Trichoderma mixed cooperative solid fermentation preparation
CN111807893A (en) * 2020-06-23 2020-10-23 秦皇岛禾苗生物技术有限公司 Special continuous cropping resistant biological bacterial fertilizer for cucumbers

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