CN114408357A - Tumor stem cell identification kit - Google Patents
Tumor stem cell identification kit Download PDFInfo
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- CN114408357A CN114408357A CN202210042166.4A CN202210042166A CN114408357A CN 114408357 A CN114408357 A CN 114408357A CN 202210042166 A CN202210042166 A CN 202210042166A CN 114408357 A CN114408357 A CN 114408357A
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- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 37
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 177
- 238000001514 detection method Methods 0.000 claims abstract description 56
- 238000012360 testing method Methods 0.000 claims description 19
- 238000004113 cell culture Methods 0.000 claims description 18
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 210000002966 serum Anatomy 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 3
- 239000001054 red pigment Substances 0.000 claims description 3
- 238000010186 staining Methods 0.000 claims description 3
- 238000010257 thawing Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 24
- 230000008569 process Effects 0.000 abstract description 22
- 230000000694 effects Effects 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 4
- 230000009471 action Effects 0.000 description 9
- 238000007789 sealing Methods 0.000 description 7
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 6
- 239000000428 dust Substances 0.000 description 6
- 238000001125 extrusion Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000003825 pressing Methods 0.000 description 5
- 206010009944 Colon cancer Diseases 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 3
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- 208000003200 Adenoma Diseases 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 2
- 201000002758 colorectal adenoma Diseases 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000013399 early diagnosis Methods 0.000 description 2
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- 230000009401 metastasis Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 206010001233 Adenoma benign Diseases 0.000 description 1
- 206010064912 Malignant transformation Diseases 0.000 description 1
- 206010066901 Treatment failure Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
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- 230000008995 epigenetic change Effects 0.000 description 1
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- 238000001179 sorption measurement Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/04—Partitions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
- B65D25/101—Springs, elastic lips, or other resilient elements to locate the articles by pressure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/02—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
- B65D81/05—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
- B65D81/051—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents using pillow-like elements filled with cushioning material, e.g. elastic foam, fabric
- B65D81/052—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents using pillow-like elements filled with cushioning material, e.g. elastic foam, fabric filled with fluid, e.g. inflatable elements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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Abstract
The invention relates to the technical field of detection kits, in particular to a tumor stem cell identification kit, which comprises a protection unit, a detection unit and a fixing unit, wherein the protection unit comprises a box body and a box cover, the box body and the box cover are made of paper, and the box cover is arranged at the end part of the upper bottom surface of the box body and is rotationally connected with the box body; according to the reagent bottle fixing device, the first fixing block is arranged in the second fixing groove in the box body, the second fixing block is arranged at the position corresponding to the outer surface of the reagent bottle, and the second fixing block embedded in the second fixing groove on the reagent bottle is combined with the first fixing block, so that the reagent bottle is kept stable in the transportation process of the reagent box, and the problems of damage, liquid leakage and the like of the reagent bottle under the vibration effect of transportation are avoided.
Description
Technical Field
The invention relates to the technical field of detection kits, in particular to a tumor stem cell identification kit.
Background
In china, colorectal cancer is the fourth most common cancer and the fifth most common cause of death, and 33.13 million new patients and 15.93 million dead patients are estimated each year, and the incidence rate thereof is continuously increased with the age of the patients. With the progress of research, it is increasingly recognized that most colorectal cancers (CRC) originate from colorectal adenomas (CRA), which are abnormal growths and processes on the surface of the colorectal and are a common disease in the digestive system. Adenomas develop slowly, sometimes undergoing malignant transformation through a series of genetic and epigenetic changes, and may take at least 10 years to progress from a detectable adenoma to cancer. However, the disease is hidden, and the clinical manifestations lack specificity and are not easy to be found. Thus, through appropriate screening and monitoring, these polyps can be detected and resected early, thereby reducing the incidence of cancer. But the early cancer screening rate is still not ideal. Early diagnosis of colorectal cancer is still one of the great challenges facing home and abroad at present. Because the examination at the cellular level has limitations, such as early detection and early warning signals of Fecal Occult Blood Test (FOBT) and invasive colonoscope combined pathological biopsy are low, the missed diagnosis rate is high, the complications are many, and the result is easily influenced by multiple factors; researchers are therefore forced to seek effective non-invasive methods from the molecular level. The appearance of the tumor stem cell theory provides a new target for the diagnosis and treatment of tumors, and the selection of surface markers of the tumor stem cells becomes the premise for early diagnosis and tumor overcoming.
Tumor Stem cells (CSCs) are present in tumor tissues in very small numbers with the potential for unlimited proliferation, are capable of differentiating into tumor cells, and are critical to the development, progression and metastasis of tumors, as well as being the root cause of tumor recurrence, metastasis and treatment failure. The tumor stem cell identification kit is used for rapidly detecting a detection object, and whether the morbidity of the colorectal cancer is caused or not is judged according to the distribution condition of the tumor stem cells in a body, so that early detection and early treatment are realized, and the mortality of colorectal cancer patients is effectively reduced.
The prior art also discloses a technical scheme of a detection kit in a patent, for example, a chinese patent with application number CN2019222944808 discloses a kit, which comprises a box body and a box cover connected with the box body, wherein the box body is sequentially provided with a standard product storage area, a reagent storage area and a configuration product storage area; the standard product storage area is provided with a plurality of first storage positions for storing quantitative standard products; the standard product storage area is provided with a cooling device; the reagent storage area and the configuration product storage area are respectively provided with a second storage position and a third storage position which are different in size; and the inner bottom surface of the box cover is adhered with a bar code for providing article information of the standard article storage area, the reagent storage area and the configuration article storage area. By the structural design, the kit is reasonable in design, convenient to operate and easy to store; however, the invention still has the defects that the box body and the box cover of the kit are usually made of paper, so that the kit can vibrate violently due to the jolt of a transport vehicle in the transportation process of the kit, and the reagent bottle is damaged, leaks and the like due to the fact that the box body and the box cover can deform under the vibration and extrusion action, so that the kit cannot be used; so that the technical solution is limited.
In view of the above, the first fixing block is arranged in the second fixing groove in the box body, the second fixing block is arranged at the position corresponding to the outer surface of the reagent bottle, and the second fixing block and the first fixing block which are embedded in the reagent bottle in the second fixing groove are combined with each other, so that the reagent bottle is kept stable in the transportation process of the reagent box, and the problems of damage, liquid leakage and the like of the reagent bottle under the vibration effect of transportation are avoided.
Disclosure of Invention
In order to make up for the defects of the prior art, the problems that the reagent box of the existing detection reagent box vibrates violently due to the jolt of a transport vehicle in the transport process, reagent bottles in the reagent box are damaged and leak liquid and the like are solved, and the reagent box cannot be used; the invention provides a tumor stem cell identification kit.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a tumor stem cell identification kit, and a detection method of the tumor stem cell identification kit comprises the following steps:
s1: opening the box cover, taking out the detection test tube from the first fixing groove of the box body, extracting 5-8 mL of a serum sample of a detection object, placing the detection test tube at room temperature for two hours or at 4 ℃ overnight, then carrying out 100 Xg centrifugal treatment on the serum sample for 20-30min, and taking supernatant fluid for detection; when the detection cannot be carried out immediately, the supernatant is stored at-20 ℃ or-80 ℃ for later use, so that repeated freeze thawing is avoided;
s2: pouring the centrifuged sample in S1 into a TSC cell culture dish, selecting Hoechst33342 fluorescent dye from a reagent bottle in a No. two fixed groove, and dropwise adding 0.4-0.6 mL of Hoechst33342 fluorescent dye into the TSC cell culture dish by using a rubber-headed dropper; slightly shaking the TSC cell culture dish, and then placing the cell culture dish at 22-35 ℃ for incubation for 20-30 minutes;
s3: after the incubation is finished, the detection personnel take out the cell culture dish and observe the staining condition of the tumor stem cells in the cell culture dish by using a fluorescence microscope to obtain a detection result;
the kit comprises a protection unit, a detection unit and a fixing unit, wherein the protection unit comprises a box body and a box cover, the box body and the box cover are made of paper, and the box cover is arranged at the end part of the upper bottom surface of the box body and is rotationally connected with the box body; a first fixing piece is arranged on the lower bottom surface of the end part of the box cover, a first clamping groove is formed in the edge part of the upper bottom surface of the box body corresponding to the first fixing piece, and a first handle is arranged on the upper surface of the box cover; the side wall of the box cover is symmetrically provided with dustproof sheets which are in contact with the outer surface of the side wall of the box body;
the detection unit comprises a detection test tube, a rubber head dropper and a reagent bottle; the rubber-head dropper is fixed on the inner surface of the box cover through a fixing ring, a first fixing groove is uniformly formed in the position, close to the left side, of the upper bottom surface of the top of the box body, a second fixing groove is uniformly formed in the position, close to the right side, of the upper bottom surface of the top of the box body, the detection test tubes are all embedded into the first fixing groove, the reagent bottles are embedded into the second fixing groove, and detection reagents are filled in the reagent bottles; the top of the second fixing groove is provided with a first groove, the area of the section of the first groove is larger than that of the section of the second fixing groove, and when a reagent bottle is fixed in the second fixing groove, a bottle cap at the top of the reagent bottle is positioned in the first groove;
the fixing unit comprises a first fixing block and a first annular groove, the surface of the inner wall of the second fixing groove is provided with the first annular groove, the first fixing block is uniformly arranged in the first annular groove, the first fixing block is an elastic block, the end part of the first fixing block extends out of the first annular groove and is in a downward bent hook shape, and the first fixing block is connected with the side wall of the second fixing groove through a spring; the reagent bottle side wall outer surface and the position that the fixed block corresponds are evenly equipped with No. two fixed blocks, No. two fixed block tip is the hook-shaped of bending upwards, and the clearance between the adjacent No. two fixed blocks is greater than the width of a fixed block.
When in use, the reagent bottle is fixed in the second fixing groove after the detection test tube and the rubber head dropper are respectively fixed; when the reagent bottle is fixed, aligning a second fixing block on the reagent bottle with a first fixing block in a second fixing groove, and then embedding the reagent bottle; when the reagent bottle moves downwards, the second fixing block on the reagent bottle is contacted with the first fixing block from the upper part, and because the end part of the first fixing block is bent downwards and the second fixing block is bent upwards, the first fixing block is extruded by the second fixing block which moves downwards, so that the first fixing block is pressed to deform and slides in the first annular groove in the direction far away from the reagent bottle, and the second fixing block smoothly passes through the first fixing block; at the moment, the end part of the first fixing block is deformed and restored and is tightly combined with the end part of the second fixing block, so that the reagent bottle is fixed; then the box cover is closed, so that the end part of the first fixing piece on the box cover is embedded into the first clamping groove, the box cover is fixed, and the interior of the kit is in a closed state; when the box cover is closed, the dustproof sheet on the box cover rotates and is in close contact with the outer surface of the side wall of the box body, so that the sealing property of the interior of the kit is enhanced, and external dust is prevented from permeating into the box body through a gap between the box cover and the box body and polluting detection equipment placed in the box body; in the transportation process of the kit, the kit may vibrate violently due to the jolt of a transportation vehicle, and the box body and the box cover are made of paper and may deform under the action of vibration and extrusion; when the reagent bottle has a movement tendency of separating from the second fixing groove under the vibration action, the first fixing block has a blocking effect on the second fixing block, so that the reagent bottle is kept stable in the second fixing groove, and the reagent bottle is prevented from falling off and being damaged in collision; when the box cover is deformed, the bottle cover is positioned in the second groove, so that the deformed box cover is obstructed by the top of the box body before contacting the bottle cover, the box cover is prevented from being deformed continuously, and the safety of the reagent bottle is further ensured; when the kit begins to use, only need rotate reagent bottle and make a fixed block be located the clearance between No. two fixed blocks, the user can take out the reagent bottle smoothly this moment, carries out tumor stem cell's testing.
Preferably, a rubber strip is arranged at the end part of the first fixing piece, and the diameter of the rubber strip is larger than the width of the first clamping groove; the side wall surface of the box body is uniformly provided with a third fixing block, and the top of the third fixing block and the corresponding part of the end part of the dustproof sheet are provided with a second clamping groove.
When the box cover is used, when the box cover is closed, the diameter of the rubber strip at the end part of the first fixing piece is larger than the width of the first clamping groove, so that when the rubber strip is embedded into the first clamping groove along with the end part of the first fixing piece, the rubber strip is pressed to deform and is tightly contacted with the inner surface of the first clamping groove, the combination of the box cover and the box body is tighter, the opening of the box cover due to vibration during transportation is avoided, and the sealing property inside the box body during transportation is further ensured; the surface of the side wall of the box body is uniformly provided with a third fixing block, so that the friction between the kit and an external sealing box in the transportation process is increased, and the sliding of the kit during the bumping of a vehicle is reduced; and when the box cover is closed, the downward-moving dustproof sheet is embedded into the second clamping groove, so that the dustproof sheet is fixed, the dustproof sheet always keeps close contact with the outer surface of the side wall of the box body when the box cover is closed, and external dust impurities are further prevented from passing through a gap between the box cover and the box body and polluting the inside of the box body.
Preferably, the bottom of the second fixing groove is provided with a first air bag, and the surface of the first air bag is in contact with the bottom of the reagent bottle; the annular air bag is arranged on the side wall of the first annular groove, a spring on the first fixing block is connected with the surface of the annular air bag, and the first air bag is connected with the annular air bag through a first pipe.
When the reagent bottle fixing device is used, when the reagent bottle is embedded into the second fixing groove, the top of the reagent bottle is pressed, the first air bag is extruded at the bottom of the reagent bottle, the first air bag is pressed to deform, and when the second fixing block on the reagent bottle passes through the first fixing block, the pressing is stopped; at the moment, the elasticity of the first air bag is recovered, so that the reagent bottle is pressed upwards, the second fixing block and the first fixing block are combined more tightly, and the reagent bottle is fixed more firmly; and when an gasbag pressurized, inside air of gasbag has partly through inside a pipe entering annular gasbag for the inside gaseous increase of annular gasbag and inflation, consequently annular gasbag is through linking to each other the fixed block of spring extrusion No. one, makes a fixed block extrusion reagent bottle when keeping closely combining with No. two fixed blocks, makes reagent bottle evenly receive the clamp force at directional center, thereby further guarantees that reagent bottle remains stable in the transportation.
Preferably, the bottom surface of the reagent bottle is in an arc shape which is bent upwards, anti-slip sheets are uniformly arranged on the bottom surface of the reagent bottle, and the end parts of the anti-slip sheets are in contact with the surface of the first air bag.
When the reagent bottle fixing device is used, the bottom of the reagent bottle is in contact with the first air bag and extrudes the first air bag, and the end part of the anti-skid sheet at the bottom of the reagent bottle is embedded into the surface of the first air bag, so that the friction force between the bottom of the reagent bottle and the first air bag is increased, and the reagent bottle is prevented from rotating to enable the second fixing block to move to a gap between the first fixing blocks in the vibration process, so that the reagent bottle is separated from the second fixing groove; therefore, the reagent bottle is kept stable under the vibration action through the combination of the anti-slip sheet and the first air bag.
Preferably, a second air bag is arranged at the end part of the second fixing block, and the surface of the second air bag is in contact with the end part of the first fixing block; and the part of the surface of the second air bag, which is close to the end part of the first fixing block, is uniformly provided with a first hole.
When the reagent bottle fixing device is used, when the first fixing block is combined with the second fixing block, the end part of the first fixing block extrudes the second air bag on the end part of the second fixing block, and the end part of the first fixing block is embedded into the surface of the second air bag, so that the friction force between the first fixing block and the second air bag is increased, and the reagent bottle is prevented from rotating and being separated from the first fixing block; in the vibration process, the second air bag has a buffering effect on the first fixing block, so that the first fixing block and the second fixing block are prevented from being damaged due to mutual collision under the vibration effect; the second air bag is deformed under pressure, so that the gas in the second air bag flows out through the first hole under pressure; consequently when a fixed block has the trend that breaks away from No. two fixed blocks, No. two gasbag deformations and resumes and inhale gas through a hole for a fixed block receives the adsorption, has guaranteed the compactness that combines between a fixed block and No. two fixed blocks, thereby has guaranteed that the reagent bottle remains stable in No. two fixed slots.
Preferably, the parts of the lower bottom surface of the first groove corresponding to the first fixing block are provided with indication arrows, the parts of the upper bottom surface of the bottle cap corresponding to the second fixing block are also provided with indication arrows, and the indication arrows are drawn by red pigments.
When the reagent bottle is used, after the reagent bottle is embedded into the second fixing groove, the specific positions of the second fixing block and the first fixing block are difficult to observe, so that the second fixing block and the first fixing block are possibly not tightly combined, and the reagent bottle is separated from the second fixing groove due to the vibration effect in the transportation process; therefore, by arranging the indicating arrow, in the process of pressing the reagent bottle to enable the reagent bottle to be embedded into the second fixing groove, the indicating arrow on the bottle cap is kept aligned with the indicating arrow on the lower bottom surface of the first groove, and therefore the first fixing block and the second fixing block are fully combined; and after the transportation is finished, when the interior of the reagent kit is checked, whether the indicating arrow on the bottle cap is still aligned with the indicating arrow on the lower bottom surface of the first groove or not can be determined, and whether the reagent bottle is fixed stably or not can be determined.
The invention has the following beneficial effects:
1. according to the tumor stem cell identification kit, the first fixing block is arranged in the second fixing groove in the kit body, the second fixing block is arranged at the position corresponding to the outer surface of the reagent bottle, and the second fixing block and the first fixing block on the reagent bottle embedded in the second fixing groove are combined with each other, so that the reagent bottle is kept stable in the transportation process of the kit, and the problems of damage, liquid leakage and the like of the reagent bottle under the vibration effect of transportation are avoided.
2. According to the tumor stem cell identification kit, the first air bag is arranged at the bottom of the second fixing groove, the annular air bag is arranged on the side wall of the first annular groove, and the first air bag enables the reagent bottle to be pressed upwards, so that the second fixing block and the first fixing block are combined more tightly, and the reagent bottle is fixed more firmly; and when an gasbag pressurized, the inside air of gasbag has partly to get into inside the annular gasbag through a pipe for annular gasbag extrudees the reagent bottle, makes the reagent bottle evenly receive the clamp force of directional center, thereby further guarantees that the reagent bottle keeps stable in the transportation.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a flow chart of a method of the present invention;
FIG. 2 is a perspective view of the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2 at A;
FIG. 4 is an enlarged view of a portion of FIG. 3 at B;
FIG. 5 is an enlarged view of a portion of FIG. 3 at C;
FIG. 6 is a perspective view of a reagent bottle according to the present invention;
in the figure: the reagent bottle comprises a box body 1, a first clamping groove 11, a first fixing groove 12, a second fixing groove 13, a first groove 131, a first fixing block 132, a first annular groove 133, a first air bag 134, an annular air bag 135, a first pipe 136, a third fixing block 14, a second clamping groove 141, a box cover 2, a first fixing piece 21, a rubber strip 211, a first handle 22, a dustproof piece 23, a detection test tube 3, a rubber head dropper 4, a fixing ring 41, a reagent bottle 5, a bottle cover 51, a second fixing block 52, a second air bag 521, a first hole 522 and an anti-skid piece 53.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to fig. 6, the detection method of the tumor stem cell identification kit of the present invention comprises the following steps:
s1: opening the box cover 2, taking out the detection test tube 3 from the first fixing groove 12 of the box body 1, extracting 5-8 mL of a serum sample of a detection object, placing the detection test tube 3 at room temperature for two hours or at 4 ℃ overnight, then carrying out 100 Xg centrifugal treatment on the serum sample for 20-30min, and taking supernatant fluid for detection; when the detection cannot be carried out immediately, the supernatant is stored at-20 ℃ or-80 ℃ for later use, so that repeated freeze thawing is avoided;
s2: pouring the centrifuged sample in S1 into a TSC cell culture dish, selecting Hoechst33342 fluorescent dye from a reagent bottle 5 in a second fixing groove 13, and dropwise adding 0.4-0.6 mL of Hoechst33342 fluorescent dye into the TSC cell culture dish by using a rubber-headed dropper 4; slightly shaking the TSC cell culture dish, and then placing the cell culture dish at 22-35 ℃ for incubation for 20-30 minutes;
s3: after the incubation is finished, the detection personnel take out the cell culture dish and observe the staining condition of the tumor stem cells in the cell culture dish by using a fluorescence microscope to obtain a detection result;
the kit comprises a protection unit, a detection unit and a fixing unit, wherein the protection unit comprises a box body 1 and a box cover 2, the box body 1 and the box cover 2 are made of paper, and the box cover 2 is arranged at the end part of the upper bottom surface of the box body 1 and is rotatably connected with the box body 1; a first fixing piece 21 is arranged on the lower bottom surface of the end part of the box cover 2, a first clamping groove 11 is arranged at the edge part of the upper bottom surface of the box body 1 corresponding to the first fixing piece 21, and a first handle 22 is arranged on the upper surface of the box cover 2; the side wall of the box cover 2 is symmetrically provided with dustproof sheets 23, and the dustproof sheets 23 are in contact with the outer surface of the side wall of the box body 1;
the detection unit comprises a detection test tube 3, a rubber head dropper 4 and a reagent bottle 5; the rubber-tipped dropper 4 is fixed on the inner surface of the box cover 2 through a fixing ring 41, a first fixing groove 12 is uniformly formed in the position, close to the left side, of the upper bottom surface of the top of the box body 1, a second fixing groove 13 is uniformly formed in the position, close to the right side, of the top of the box body 1, the detection test tubes 3 are all embedded into the first fixing groove 12, the reagent bottles 5 are embedded into the second fixing groove 13, and detection reagents are filled in the reagent bottles 5; the top of the second fixing groove 13 is provided with a first groove 131, the area of the section of the first groove 131 is larger than that of the section of the second fixing groove 13, and when the reagent bottle 5 is fixed in the second fixing groove 13, the bottle cap 51 at the top of the reagent bottle 5 is positioned in the first groove 131;
the fixing unit comprises a first fixing block 132 and a first annular groove 133, the first annular groove 133 is formed in the inner wall surface of the second fixing groove 13, the first fixing block 132 is uniformly arranged in the first annular groove 133, the first fixing block 132 is an elastic block, the end part of the first fixing block 132 extends out of the first annular groove 133 and is in a hook shape bent downwards, and the first fixing block 132 is connected with the side wall of the second fixing groove 13 through a spring; the outer surface of the side wall of the reagent bottle 5 corresponding to the first fixing block 132 is uniformly provided with a second fixing block 52, the end part of the second fixing block 52 is in the shape of an upward bent hook, and the gap between the adjacent second fixing blocks 52 is larger than the width of the first fixing block 132.
When in use, the reagent bottle 5 is fixed in the second fixing groove 13 after the detection test tube 3 and the rubber dropper 4 are respectively fixed; when the reagent bottle 5 is fixed, the second fixing block 52 on the reagent bottle 5 is aligned with the first fixing block 132 in the second fixing groove 13, and then the reagent bottle 5 is embedded; when the reagent bottle 5 moves downwards, the second fixing block 52 on the reagent bottle 5 is contacted with the first fixing block 132 from the upper part, because the end part of the first fixing block 132 is bent downwards, and the second fixing block 52 is bent upwards, the first fixing block 132 is extruded by the second fixing block 52 moving downwards, so that the first fixing block 132 is pressed and deformed and slides in the first annular groove 133 in the direction away from the reagent bottle 5, and the second fixing block 52 smoothly passes through the first fixing block 132; at the moment, the end part of the first fixing block 132 is deformed and restored and is tightly combined with the end part of the second fixing block 52, so that the reagent bottle 5 is fixed; then, the box cover 2 is closed, so that the end part of the first fixing piece 21 on the box cover 2 is embedded into the first clamping groove 11, the box cover 2 is fixed, and the inside of the reagent box is in a closed state; when the box cover 2 is closed, the dustproof sheet 23 on the box cover 2 rotates and is in close contact with the outer surface of the side wall of the box body 1, so that the sealing performance of the interior of the kit is enhanced, and external dust is prevented from permeating into the box body 1 through a gap between the box cover 2 and the box body 1 and polluting detection equipment placed in the box body 1; in the transportation process of the reagent kit, the reagent kit may vibrate violently due to the jolt of a transportation vehicle, and the box body 1 and the box cover 2 are made of paper and may deform under the action of vibration and extrusion; when the reagent bottle 5 moves away from the second fixing groove 13 under the vibration action, the first fixing block 132 has a blocking effect on the second fixing block 52, so that the reagent bottle 5 is kept stable in the second fixing groove 13, and the reagent bottle 5 is prevented from falling off and being damaged in collision; when the box cover 2 is deformed, the bottle cover 51 is positioned in the second groove, so that the deformed box cover 2 is obstructed by the top of the box body 1 before contacting the bottle cover 51, the box cover 2 is prevented from being deformed continuously, and the safety of the reagent bottle 5 is further ensured; when the kit starts to be used, the user can smoothly take out the reagent bottle 5 to perform a detection test of the tumor stem cells only by rotating the reagent bottle 5 to enable the first fixing block 132 to be positioned in the gap between the second fixing blocks 52.
As an embodiment of the invention, a rubber strip 211 is arranged at the end of the first fixing piece 21, and the diameter of the rubber strip 211 is larger than the width of the first clamping groove 11; the lateral wall surface of the box body 1 is uniformly provided with a third fixing block 14, and the top of the third fixing block 14 corresponding to the end part of the dustproof sheet 23 is provided with a second clamping groove 141.
When the box cover 2 is closed, the diameter of the rubber strip 211 at the end part of the first fixing piece 21 is larger than the width of the first clamping groove 11, so that when the rubber strip 211 is embedded into the first clamping groove 11 along with the end part of the first fixing piece 21, the rubber strip 211 is pressed to deform and is in close contact with the inner surface of the first clamping groove 11, the combination of the box cover 2 and the box body 1 is tighter, the box cover 2 is prevented from being opened due to vibration during transportation, and the sealing performance inside the box body 1 is further ensured during transportation; the surface of the side wall of the box body 1 is uniformly provided with a third fixing block 14, so that the friction between the kit and an external closed box in the transportation process is increased, and the sliding of the kit during the bumping of a vehicle is reduced; and when lid 2 seals, the dustproof piece 23 that moves down imbeds No. two draw-in grooves 141 for dustproof piece 23 receives fixedly, consequently dustproof piece 23 keeps in close contact with box body 1 lateral wall surface all the time when lid 2 seals, further avoids outside dust debris to pass through the clearance between lid 2 and the box body 1 and pollute the box body 1 inside.
As an embodiment of the invention, a first air bag 134 is arranged at the bottom of the second fixing groove 13, and the surface of the first air bag 134 is in contact with the bottom of the reagent bottle 5; an annular air bag 135 is arranged on the side wall of the first annular groove 133, a spring on the first fixing block 132 is connected with the surface of the annular air bag 135, and the first air bag 134 and the annular air bag 135 are connected through a first pipe 136.
When the reagent bottle 5 is embedded into the second fixing groove 13, the top of the reagent bottle 5 is pressed, the first air bag 134 is extruded at the bottom of the reagent bottle 5, the first air bag 134 is pressed to deform, and when the second fixing block 52 on the reagent bottle 5 passes through the first fixing block 132, the pressing is stopped; at this time, the first air bag 134 is elastically restored, so that the reagent bottle 5 is pressed upwards, the second fixing block 52 and the first fixing block 132 are combined more tightly, and the reagent bottle 5 is fixed more firmly; and when first gasbag 134 pressurized, the inside air of first gasbag 134 has some to enter annular gasbag 135 through a pipe 136 inside for annular gasbag 135 inside gaseous increase and inflation, therefore annular gasbag 135 extrudees No. one fixed block 132 through the spring that links to each other, makes No. one fixed block 132 extrude reagent bottle 5 when keeping closely combining with No. two fixed blocks 52, makes reagent bottle 5 evenly receive the clamp force of directional center, thereby further guarantees that reagent bottle 5 keeps stable in the transportation.
As an embodiment of the invention, the bottom surface of the reagent bottle 5 is an arc shape which is bent upwards, and the bottom surface of the reagent bottle 5 is uniformly provided with anti-slip sheets 53, and the end parts of the anti-slip sheets 53 are in contact with the surface of the first air bag 134.
When the reagent bottle fixing device is used, when the bottom of the reagent bottle 5 is in contact with the first air bag 134 and extrudes the first air bag 134, the end part of the anti-skidding sheet 53 at the bottom of the reagent bottle 5 is embedded into the surface of the first air bag 134, so that the friction force between the bottom of the reagent bottle 5 and the first air bag 134 is increased, and the phenomenon that the reagent bottle 5 rotates to enable the second fixing block 52 to move to a gap between the first fixing blocks 132 in the vibration process is avoided, so that the reagent bottle 5 is separated from the second fixing groove 13; therefore, the reagent bottle 5 is kept stable under the vibration action by the combination of the anti-slip sheet 53 and the first air bag 134.
As an embodiment of the invention, a second air bag 521 is arranged at the end of the second fixed block 52, and the surface of the second air bag 521 is in contact with the end of the first fixed block 132; and a first hole 522 is uniformly arranged on the surface of the second air bag 521 near the end part of the first fixing block 132.
When the reagent bottle is used, when the first fixing block 132 is combined with the second fixing block 52, the end part of the first fixing block 132 extrudes the second air bag 521 on the end part of the second fixing block 52, and the end part of the first fixing block 132 is embedded into the surface of the second air bag 521, so that the friction force between the first fixing block 132 and the second air bag 521 is increased, and the reagent bottle 5 is prevented from rotating and being separated from the first fixing block 132; in the vibration process, the second air bag 521 plays a role in buffering the first fixing block 132, so that the first fixing block 132 and the second fixing block 52 are prevented from being damaged due to mutual collision under the vibration effect; and the second airbag 521 is deformed under pressure, so that the gas inside the second airbag 521 flows out through the first hole 522 under pressure; therefore, when the first fixing block 132 tends to be separated from the second fixing block 52, the second air bag 521 deforms and recovers and sucks air through the first hole 522, so that the first fixing block 132 is adsorbed, the tightness of combination between the first fixing block 132 and the second fixing block 52 is guaranteed, and the reagent bottle 5 is guaranteed to be stable in the second fixing groove 13.
In an embodiment of the present invention, indicating arrows are disposed on the lower bottom surface of the first groove 131 and the corresponding portion of the first fixing block 132, and indicating arrows are also disposed on the upper bottom surface of the bottle cap 51 and the corresponding portion of the second fixing block 52, and are depicted by using red pigment.
When the reagent bottle 5 is inserted into the second fixing groove 13, the specific positions of the second fixing block 52 and the first fixing block 132 are difficult to observe, so that the second fixing block 52 and the first fixing block 132 are not tightly combined, and the reagent bottle 5 is separated from the second fixing groove 13 due to vibration in the transportation process; therefore, by arranging the indicating arrow, in the process of pressing the reagent bottle 5 to enable the reagent bottle 5 to be embedded into the second fixing groove 13, the indicating arrow on the bottle cap 51 is kept aligned with the indicating arrow on the lower bottom surface of the first groove 131, and therefore the first fixing block 132 is fully combined with the second fixing block 52; and after the transportation is finished, when the inside of the reagent kit is checked, whether the reagent bottle 5 is fixed stably can be determined only by observing whether the indication arrow on the bottle cap 51 is still aligned with the indication arrow on the lower bottom surface of the first groove 131.
When in use, the reagent bottle 5 is fixed in the second fixing groove 13 after the detection test tube 3 and the rubber dropper 4 are respectively fixed; when the reagent bottle 5 is fixed, the second fixing block 52 on the reagent bottle 5 is aligned with the first fixing block 132 in the second fixing groove 13, and then the reagent bottle 5 is embedded; when the reagent bottle 5 moves downwards, the second fixing block 52 on the reagent bottle 5 is contacted with the first fixing block 132 from the upper part, because the end part of the first fixing block 132 is bent downwards, and the second fixing block 52 is bent upwards, the first fixing block 132 is extruded by the second fixing block 52 moving downwards, so that the first fixing block 132 is pressed and deformed and slides in the first annular groove 133 in the direction away from the reagent bottle 5, and the second fixing block 52 smoothly passes through the first fixing block 132; at the moment, the end part of the first fixing block 132 is deformed and restored and is tightly combined with the end part of the second fixing block 52, so that the reagent bottle 5 is fixed; then, the box cover 2 is closed, so that the end part of the first fixing piece 21 on the box cover 2 is embedded into the first clamping groove 11, the box cover 2 is fixed, and the inside of the reagent box is in a closed state; when the box cover 2 is closed, the dustproof sheet 23 on the box cover 2 rotates and is in close contact with the outer surface of the side wall of the box body 1, so that the sealing performance of the interior of the kit is enhanced, and external dust is prevented from permeating into the box body 1 through a gap between the box cover 2 and the box body 1 and polluting detection equipment placed in the box body 1; in the transportation process of the reagent kit, the reagent kit may vibrate violently due to the jolt of a transportation vehicle, and the box body 1 and the box cover 2 are made of paper and may deform under the action of vibration and extrusion; when the reagent bottle 5 moves away from the second fixing groove 13 under the vibration action, the first fixing block 132 has a blocking effect on the second fixing block 52, so that the reagent bottle 5 is kept stable in the second fixing groove 13, and the reagent bottle 5 is prevented from falling off and being damaged in collision; when the box cover 2 is deformed, the bottle cover 51 is positioned in the second groove, so that the deformed box cover 2 is obstructed by the top of the box body 1 before contacting the bottle cover 51, the box cover 2 is prevented from being deformed continuously, and the safety of the reagent bottle 5 is further ensured; when the kit is used, the reagent bottle 5 is only required to be rotated to enable the first fixing block 132 to be positioned in the gap between the second fixing blocks 52, and at the moment, a user can smoothly take out the reagent bottle 5 to perform a detection test on the tumor stem cells; when the box cover 2 is closed, the diameter of the rubber strip 211 at the end part of the first fixing piece 21 is larger than the width of the first clamping groove 11, so that when the rubber strip 211 is embedded into the first clamping groove 11 along with the end part of the first fixing piece 21, the rubber strip 211 is pressed to deform and is in close contact with the inner surface of the first clamping groove 11, the combination of the box cover 2 and the box body 1 is tighter, the box cover 2 is prevented from being opened due to vibration during transportation, and the sealing performance inside the box body 1 is further ensured during transportation; the surface of the side wall of the box body 1 is uniformly provided with a third fixing block 14, so that the friction between the kit and an external closed box in the transportation process is increased, and the sliding of the kit during the bumping of a vehicle is reduced; when the box cover 2 is closed, the downward-moving dustproof sheet 23 is embedded into the second clamping groove 141, so that the dustproof sheet 23 is fixed, when the box cover 2 is closed, the dustproof sheet 23 is always in close contact with the outer surface of the side wall of the box body 1, and external dust impurities are further prevented from passing through a gap between the box cover 2 and the box body 1 and polluting the inside of the box body 1; when the reagent bottle 5 is embedded into the second fixing groove 13, the top of the reagent bottle 5 is pressed, the first air bag 134 is extruded at the bottom of the reagent bottle 5, so that the first air bag 134 is pressed and deformed, and when the second fixing block 52 on the reagent bottle 5 passes through the first fixing block 132, the pressing is stopped; at this time, the first air bag 134 is elastically restored, so that the reagent bottle 5 is pressed upwards, the second fixing block 52 and the first fixing block 132 are combined more tightly, and the reagent bottle 5 is fixed more firmly; when the first air bag 134 is pressed, a part of air in the first air bag 134 enters the annular air bag 135 through the first pipe 136, so that the air in the annular air bag 135 is increased and expanded, the annular air bag 135 extrudes the first fixing block 132 through the connecting spring, the first fixing block 132 is tightly combined with the second fixing block 52, and meanwhile, the reagent bottle 5 is extruded, so that the reagent bottle 5 is uniformly clamped by a clamping force pointing to the center, and the reagent bottle 5 is further ensured to be kept stable in the transportation process; when the first fixing block 132 is combined with the second fixing block 52, the end part of the first fixing block 132 extrudes the second air bag 521 on the end part of the second fixing block 52, and the end part of the first fixing block 132 is embedded into the surface of the second air bag 521, so that the friction force between the first fixing block 132 and the second air bag 521 is increased, and the reagent bottle 5 is prevented from rotating and being separated from the first fixing block 132; in the vibration process, the second air bag 521 plays a role in buffering the first fixing block 132, so that the first fixing block 132 and the second fixing block 52 are prevented from being damaged due to mutual collision under the vibration effect; and the second airbag 521 is deformed under pressure, so that the gas inside the second airbag 521 flows out through the first hole 522 under pressure; therefore, when the first fixing block 132 tends to be separated from the second fixing block 52, the second air bag 521 deforms and recovers and sucks air through the first hole 522, so that the first fixing block 132 is adsorbed, the tightness of combination between the first fixing block 132 and the second fixing block 52 is guaranteed, and the reagent bottle 5 is guaranteed to be stable in the second fixing groove 13.
The front, the back, the left, the right, the upper and the lower are all based on the figure 2 in the attached drawings of the specification, according to the standard of the observation angle of a person, the side of the device facing an observer is defined as the front, the left side of the observer is defined as the left, and the like.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplifying the description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the scope of the present invention.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A tumor stem cell identification kit is characterized in that: the detection method of the tumor stem cell identification kit comprises the following steps:
s1: opening the box cover (2), taking out the detection test tube (3) from the first fixing groove (12) of the box body (1), extracting 5-8 mL of a serum sample of a detection object, placing the detection test tube (3) at room temperature for two hours or at 4 ℃ overnight, then carrying out 100 Xg centrifugal treatment on the serum sample for 20-30min, and taking out supernatant fluid for detection; when the detection cannot be carried out immediately, the supernatant is stored at-20 ℃ or-80 ℃ for later use, so that repeated freeze thawing is avoided;
s2: pouring the centrifuged sample in S1 into a TSC cell culture dish, selecting Hoechst33342 fluorescent dye from a reagent bottle (5) in a second fixed groove (13), and dripping 0.4-0.6 mL of Hoechst33342 fluorescent dye into the TSC cell culture dish by using a rubber head dropper (4); slightly shaking the TSC cell culture dish, and then placing the cell culture dish at 22-35 ℃ for incubation for 20-30 minutes;
s3: after the incubation is finished, the detection personnel take out the cell culture dish and observe the staining condition of the tumor stem cells in the cell culture dish by using a fluorescence microscope to obtain a detection result;
the kit comprises a protection unit, a detection unit and a fixing unit, wherein the protection unit comprises a box body (1) and a box cover (2), the box body (1) and the box cover (2) are made of paper, and the box cover (2) is installed at the end part of the upper bottom surface of the box body (1) and is rotatably connected with the box body (1); a first fixing piece (21) is arranged on the lower bottom surface of the end part of the box cover (2), a first clamping groove (11) is arranged at the edge part of the upper bottom surface of the box body (1) corresponding to the first fixing piece (21), and a first handle (22) is arranged on the upper surface of the box cover (2); the side wall of the box cover (2) is symmetrically provided with dustproof sheets (23), and the dustproof sheets (23) are in contact with the outer surface of the side wall of the box body (1);
the detection unit comprises a detection test tube (3), a rubber head dropper (4) and a reagent bottle (5); the rubber head dropper (4) is fixed on the inner surface of the box cover (2) through a fixing ring (41), a first fixing groove (12) is uniformly formed in the position, close to the left side, of the upper bottom surface of the top of the box body (1), a second fixing groove (13) is uniformly formed in the position, close to the right side, of the top of the box body, the detection test tubes (3) are embedded into the first fixing groove (12), the reagent bottles (5) are embedded into the second fixing groove (13), and detection reagents are filled in the reagent bottles (5); the top of the second fixing groove (13) is provided with a first groove (131), the area of the section of the first groove (131) is larger than that of the section of the second fixing groove (13), and when the reagent bottle (5) is fixed in the second fixing groove (13), the bottle cap (51) at the top of the reagent bottle (5) is positioned in the first groove (131);
the fixing unit comprises a first fixing block (132) and a first annular groove (133), the first annular groove (133) is formed in the inner wall surface of the second fixing groove (13), the first fixing block (132) is uniformly arranged in the first annular groove (133), the first fixing block (132) is an elastic block, the end part of the first fixing block (132) extends out of the first annular groove (133) and is in a downward bent hook shape, and the first fixing block (132) is connected with the side wall of the second fixing groove (13) through a spring; the reagent bottle (5) is characterized in that a second fixing block (52) is uniformly arranged on the outer surface of the side wall of the reagent bottle (5) corresponding to the first fixing block (132), the end part of the second fixing block (52) is in an upward bent hook shape, and the gap between every two adjacent fixing blocks (52) is larger than the width of the first fixing block (132).
2. The kit for identifying tumor stem cells according to claim 1, wherein: the end part of the first fixing piece (21) is provided with a rubber strip (211), and the diameter of the rubber strip (211) is larger than the width of the first clamping groove (11); the side wall surface of the box body (1) is uniformly provided with a third fixing block (14), and the top of the third fixing block (14) corresponding to the end part of the dustproof sheet (23) is provided with a second clamping groove (141).
3. The tumor stem cell identification kit according to claim 2, wherein: a first air bag (134) is arranged at the bottom of the second fixing groove (13), and the surface of the first air bag (134) is in contact with the bottom of the reagent bottle (5); an annular air bag (135) is arranged on the side wall of the first annular groove (133), a spring on the first fixing block (132) is connected with the surface of the annular air bag (135), and the first air bag (134) is connected with the annular air bag (135) through a first pipe (136).
4. The kit for identifying tumor stem cells according to claim 3, wherein: the reagent bottle (5) bottom surface is the arc of kickup, just reagent bottle (5) bottom surface is last evenly to be equipped with antiskid sheet (53), antiskid sheet (53) tip with gasbag (134) surface contact No. one.
5. The kit for identifying tumor stem cells according to claim 4, wherein: a second air bag (521) is arranged at the end part of the second fixing block (52), and the surface of the second air bag (521) is contacted with the end part of the first fixing block (132); and a first hole (522) is uniformly formed in the part, close to the end part of the first fixing block (132), on the surface of the second air bag (521).
6. The kit for identifying tumor stem cells according to claim 5, wherein: the parts of the lower bottom surface of the first groove (131) corresponding to the first fixing block (132) are provided with indication arrows, the parts of the upper bottom surface of the bottle cap (51) corresponding to the second fixing block (52) are also provided with indication arrows, and the indication arrows are drawn by red pigment.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210042166.4A CN114408357B (en) | 2022-01-14 | 2022-01-14 | Tumor stem cell identification kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210042166.4A CN114408357B (en) | 2022-01-14 | 2022-01-14 | Tumor stem cell identification kit |
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| CN114408357A true CN114408357A (en) | 2022-04-29 |
| CN114408357B CN114408357B (en) | 2022-10-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115535416A (en) * | 2022-11-29 | 2022-12-30 | 卡力(烟台)农业发展有限公司 | Soil remediation improves microbial inoculum transport container |
| CN115783488A (en) * | 2023-02-09 | 2023-03-14 | 黑龙江省农业科学院经济作物研究所 | Potato disease detects and stores box with virus transportation |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3157029U (en) * | 2009-11-10 | 2010-01-28 | 国立大学法人東京海洋大学 | Cell experiment kit |
| CN206843479U (en) * | 2017-06-12 | 2018-01-05 | 益基武汉生物科技有限公司 | A kind of ovarian cancer gene detection kit |
| CN208412604U (en) * | 2018-05-12 | 2019-01-22 | 北京科奥明生物技术有限公司 | A kind of detection kit |
| CN209905435U (en) * | 2019-04-03 | 2020-01-07 | 天津悦和康生物技术有限公司 | Prealbumin determination kit |
| CN210338779U (en) * | 2019-06-27 | 2020-04-17 | 成都晴星科技有限公司 | Detection kit convenient to transportation |
| CN212354887U (en) * | 2020-06-02 | 2021-01-15 | 河南申友医学检验所有限公司 | Nucleic acid extraction kit for processing large-volume samples |
| CN112678322A (en) * | 2020-12-25 | 2021-04-20 | 哈尔滨钜仁生物科技有限公司 | Kit for cell detection |
| CN112918876A (en) * | 2021-04-20 | 2021-06-08 | 南京工业职业技术大学 | It has aerogel and shock-absorbing cold chain transport case of controllable temperature to fill |
| CN213975086U (en) * | 2020-09-17 | 2021-08-17 | 谷晓云 | Specimen submission box for gene detection |
| CN214397756U (en) * | 2020-12-07 | 2021-10-15 | 上海卡敏生物科技有限公司 | DNA purification recovery kit convenient to wash |
-
2022
- 2022-01-14 CN CN202210042166.4A patent/CN114408357B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3157029U (en) * | 2009-11-10 | 2010-01-28 | 国立大学法人東京海洋大学 | Cell experiment kit |
| CN206843479U (en) * | 2017-06-12 | 2018-01-05 | 益基武汉生物科技有限公司 | A kind of ovarian cancer gene detection kit |
| CN208412604U (en) * | 2018-05-12 | 2019-01-22 | 北京科奥明生物技术有限公司 | A kind of detection kit |
| CN209905435U (en) * | 2019-04-03 | 2020-01-07 | 天津悦和康生物技术有限公司 | Prealbumin determination kit |
| CN210338779U (en) * | 2019-06-27 | 2020-04-17 | 成都晴星科技有限公司 | Detection kit convenient to transportation |
| CN212354887U (en) * | 2020-06-02 | 2021-01-15 | 河南申友医学检验所有限公司 | Nucleic acid extraction kit for processing large-volume samples |
| CN213975086U (en) * | 2020-09-17 | 2021-08-17 | 谷晓云 | Specimen submission box for gene detection |
| CN214397756U (en) * | 2020-12-07 | 2021-10-15 | 上海卡敏生物科技有限公司 | DNA purification recovery kit convenient to wash |
| CN112678322A (en) * | 2020-12-25 | 2021-04-20 | 哈尔滨钜仁生物科技有限公司 | Kit for cell detection |
| CN112918876A (en) * | 2021-04-20 | 2021-06-08 | 南京工业职业技术大学 | It has aerogel and shock-absorbing cold chain transport case of controllable temperature to fill |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115535416A (en) * | 2022-11-29 | 2022-12-30 | 卡力(烟台)农业发展有限公司 | Soil remediation improves microbial inoculum transport container |
| CN115535416B (en) * | 2022-11-29 | 2023-01-31 | 卡力(烟台)农业发展有限公司 | Soil remediation improves microbial inoculum transport container |
| CN115783488A (en) * | 2023-02-09 | 2023-03-14 | 黑龙江省农业科学院经济作物研究所 | Potato disease detects and stores box with virus transportation |
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| CN114408357B (en) | 2022-10-04 |
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