Tumor somatic mutation site flow detection and analysis device
Technical Field
The utility model belongs to the technical field of the analysis and detection technique and specifically relates to a tumour somatic mutation site flow formula detection and analysis device.
Background
Flow Cytometry (FCM) is a modern cell analysis technology developed in the seventies, which integrates computer technology, laser technology, fluid mechanics, cytochemistry and cellular immunology, and has the functions of analyzing and sorting cells. The cell size and the properties of internal particles can be measured, the cell surface and cytoplasm antigens, the intracellular DNA and RNA content and the like can be detected, the group cells can be analyzed on the single cell level, a large number of cells can be detected and analyzed in a short time, data is collected, stored and processed, multi-parameter quantitative analysis is carried out, the cell size and the properties of internal particles can be measured, a certain subset of cells can be classified and collected, and the separation purity is higher than 95%. Flow cytometry is widely used in hematology, immunology, oncology, pharmacology, molecular biology and other disciplines.
The application of flow cytometry in hematology mainly relates to the immunophenotyping analysis of white blood cells, red blood cells, platelets and leukemia, myeloma and lymphoma cells, CD34+ cell counting, lymphocyte subpopulation and immune function detection, cell cycle and DNA ploidy analysis, apoptosis and other aspects. Immunofluorescence staining is a key step for performing cellular immune marker analysis by using a flow cytometer, and mainly comprises a direct immunofluorescence staining method and an indirect immunofluorescence staining method. The indirect immunofluorescence staining is to adopt a specific primary antibody without fluorescence labeling to react with a specimen to be detected, after hemolysis and washing, the primary antibody with fluorescence labeling is added, and after incubation and washing, the detection is carried out on a computer. The direct immunofluorescence staining is that a specific antibody connected with a fluorescent dye is added during marking, and the method has the advantages of simple operation, low background fluorescence, high signal-to-noise ratio, simultaneous marking of multi-color antibodies and the like, so that the method is widely applied to various laboratories at present and is a multi-color direct immunofluorescence marking method.
Immunofluorescence staining can be divided into cell surface and intracellular antigen staining, depending on the location of the labeled antigen. The cell surface antigen marker is mainly synthesized from the inside of cells and then expressed on the surface of cell membranes, and the flow cytometry phenotypic analysis experiment operation of various cell surface antigens or receptors is relatively simple, so that the cell surface antigen marker is most widely applied to clinical application. In addition to molecules expressed on the cell surface, a large number of molecules or cytokines to be secreted, etc., which play important physiological roles, are present in the cell. In contrast to the labeling of cell membrane surface antigen markers, labeling of intracellular or nuclear antigens and the measurement of intracellular cytokines first require a transmembrane process, and the experimental environment required for different antigen labeling is different. For example, in the case of cell surface antigen markers, intracellular or nuclear antigen markers, the fluorescent dye is incubated under "incubation in the dark at room temperature for 15 min", and in the case of intracellular cytokine staining, the fluorescent dye is incubated under "incubation in the dark at 4 ℃ for 30 min".
The fluorescent staining of single cell suspension is related to the accuracy of flow analysis, the characteristics and dose-effect relationship of the dye should be paid special attention, and the immunofluorescent staining also involves the problems of antibody specificity and titer, and should be carried out strictly according to the requirements of experimental operation. The rising of the environmental temperature has obvious influence on the fluorescent dyeing, the viscosity of the solution can be increased, the power of fluorescent dye molecules is increased, the possibility of fluorescent quenching is increased, and the photon yield of the fluorescent molecules is reduced. If the temperature is kept below 20 ℃, the change of photon yield is not influenced, and the light irradiation time of the dyed sample is reduced as much as possible, so that the fluorescence intensity during detection is not influenced.
In the sample preparation process for flow analysis, the requirements of the experimental environment on temperature are different according to different labeled antigen sites. After the sample is dyed by adding the fluorescent dye, the sample is placed in a dark environment for incubation, and the process from dyeing to the intermediate experimental step of on-machine inspection is reduced as much as possible, so that the light irradiation time of the dyed sample is reduced, and the fluorescent intensity during the inspection is not influenced. Currently, biological laboratories perform this operation on conventional test tube racks or flow tube racks, and in order to achieve the light-shielding effect, the test tube racks or flow tube racks containing samples need to be transferred to light-shielding places or containers, such as drawers, cabinets, and the like. If the experiment is required to be carried out in a temperature environment of 4 ℃, the test tube rack or the flow tube rack with the samples is also required to be transferred into a refrigerating chamber of a refrigerator. The above operations not only make the experiment operation more complicated, but also prolong the light irradiation time of the stained sample, increase the risk of quenching of fluorescent molecules, cause the distortion of the detection result, even cause the experiment failure, and seriously affect the subsequent research or clinical diagnosis.
In summary, it is very important to provide an experimental apparatus capable of providing a light-shielding and suitable temperature environment for flow cytometry analysis to obtain an accurate and effective flow detection result.
SUMMERY OF THE UTILITY MODEL
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments, and in this section as well as in the abstract of the specification and the title of the application may be somewhat simplified or omitted to avoid obscuring the purpose of this section, the abstract of the specification and the title of the application, and such simplification or omission may not be used to limit the scope of the invention.
The present invention has been made in view of the above and/or other problems occurring in the prior art.
Therefore, the utility model aims to provide a tumour somatic mutation site STREAMING detection analysis device, the device can all need to go on in corresponding suitable light-resistant and temperature environment at steps such as fluorescence staining in the sample preparation process to obtain more accurate effectual STREAMING testing result.
In order to solve the technical problem, the utility model provides a following technical scheme: a flow detection and analysis device for tumor somatic mutation sites comprises,
the box body is provided with an accommodating cavity and a heat preservation cavity from the side, the accommodating cavity and the heat preservation cavity are separated by a partition plate, a first drawer is arranged in the accommodating cavity, and a second drawer is arranged in the heat preservation cavity;
the bottom of the heat preservation cavity is provided with a bottom plate, a water accumulation cavity is arranged under the bottom plate, the bottom surface of the water accumulation cavity is an inclined plane, a drain hole is formed in one side, located at the lower part of the inclined plane, of the side wall of the water accumulation cavity, a drain pipe is arranged at the position, corresponding to the drain hole, of the outer side of the box body, a blocking nut is arranged at the end part of the drain pipe, and the blocking nut is in threaded connection with.
As a preferred embodiment of the tumor somatic mutation site flow detection and analysis device of the present invention, wherein: a first supporting plate is arranged above the partition plate, and the first drawer is arranged on the first supporting plate.
As a preferred embodiment of the tumor somatic mutation site flow detection and analysis device of the present invention, wherein: the bottom plate is provided with a second supporting plate, and the second drawer is arranged on the second supporting plate in a lapping mode.
As a preferred embodiment of the tumor somatic mutation site flow detection and analysis device of the present invention, wherein: the first drawer bottom plate is provided with a first through hole, and the end face of the first drawer is provided with a first handle.
As a preferred embodiment of the tumor somatic mutation site flow detection and analysis device of the present invention, wherein: and a second through hole is formed in the second drawer bottom plate, and a second handle is arranged on the end face of the second drawer.
As a preferred embodiment of the tumor somatic mutation site flow detection and analysis device of the present invention, wherein: the partition plate is provided with a third through hole, and the bottom plate is provided with a fourth through hole.
The utility model has the advantages that: the device can carry out steps such as fluorescent staining in the sample preparation process in a corresponding proper environment with light and temperature, so as to obtain a more accurate and effective flow detection result.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive labor. Wherein:
fig. 1 is a schematic diagram of an overall explosion structure of a flow detection and analysis device for tumor somatic mutation sites according to an embodiment of the present invention;
fig. 2 is a schematic cross-sectional view of a cartridge in a flow-type detection and analysis device for a tumor somatic mutation site according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a drain pipe and a blocking nut in a flow detection and analysis device for tumor somatic mutation sites according to an embodiment of the present invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, embodiments accompanying the present invention are described in detail below with reference to the accompanying drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be implemented in other ways different from the specific details set forth herein, and one skilled in the art may similarly generalize the present invention without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Next, the present invention will be described in detail with reference to the schematic drawings, and in the detailed description of the embodiments of the present invention, for convenience of illustration, the sectional view showing the device structure will not be enlarged partially according to the general scale, and the schematic drawings are only examples, and should not limit the scope of the present invention. In addition, the three-dimensional dimensions of length, width and depth should be included in the actual fabrication.
Further still, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with at least one implementation of the invention is included. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Referring to fig. 1, the present embodiment provides a flow assay device for tumor somatic mutation sites, comprising,
the box comprises a box body 1, wherein the box body 1 is provided with an accommodating cavity 2 and a heat preservation cavity 3 from the side, the accommodating cavity 2 and the heat preservation cavity 3 are separated by a partition plate 4, a first drawer 5 is arranged in the accommodating cavity 2, and a second drawer 6 is arranged in the heat preservation cavity 3;
the bottom of the heat preservation cavity 3 is provided with a bottom plate 7, a water accumulation cavity 8 is arranged under the bottom plate 7, the bottom surface of the water accumulation cavity 8 is an inclined surface 9, a drain hole is arranged on one side of the side wall of the water accumulation cavity 8, which is positioned at the lower part of the inclined surface 9, a drain pipe 11 is arranged at the position, corresponding to the drain hole, of the outer side of the box body 1, a plugging nut 12 is arranged at the end part of the drain pipe 11, and the plugging.
This device is used for keeping out of the sun, keeps warm and deposits and is used for tumour somatic mutation site STREAMING to detect used sample, and is concrete, and the sample is placed in first drawer 5, places in second drawer 6 as ice-cube or ice bag that the cooling was used, and the setting of ponding chamber 8 is that the appetite has saved the ice-cube and melts or the adnexed water droplet on the ice bag to discharge out through drain pipe 11 jointly.
The sample is placed in the first drawer 5, the sample cannot be illuminated due to the arrangement of a sealed environment, and the temperature of the environment where the sample is located is kept appropriate due to the heat preservation effect of ice cubes, ice bags and the like arranged in the second drawer 6.
The drain pipe 11 is sealed by a sealing nut 12, and a sealing ring is further provided in the sealing nut 12 to prevent water leakage.
A first supporting plate 41 is arranged above the partition plate 4, and the first drawer 5 is lapped on the first supporting plate 41.
The bottom plate 7 is provided with a second support plate 71, and the second drawer 6 is placed on the second support plate 71.
The bottom plate of the first drawer 5 is provided with a first through hole 51, and the end surface of the first drawer 5 is provided with a first handle 52.
The bottom plate of the second drawer 6 is provided with a second through hole 61, and the end surface of the second drawer 6 is provided with a second handle 62.
The partition plate 4 is provided with a third through hole 42, and the bottom plate 7 is provided with a fourth through hole 72.
The first supporting plate 41 leaves a certain space between the partition plate 4 and the bottom plate of the first drawer 5, so that the first through hole 51 and the third through hole 42 are prevented from being blocked and blocking temperature conduction due to the fact that the partition plate 4 is attached to the first drawer 5. The second support plate 71 functions as the first support plate 41.
The arrangement of the first through hole 51, the second through hole 61, the third through hole 42 and the fourth through hole 72 ensures that the temperature conduction is not hindered in order to communicate the air in the accommodating cavity 2, the heat preservation cavity 3, the first drawer 5 and the second drawer 6.
It is important to note that the construction and arrangement of the present application as shown in the various exemplary embodiments is illustrative only. Although only a few embodiments have been described in detail in this disclosure, those skilled in the art who review this disclosure will readily appreciate that many modifications are possible (e.g., variations in sizes, dimensions, structures, shapes and proportions of the various elements, values of parameters (e.g., temperatures, pressures, etc.), mounting arrangements, use of materials, colors, orientations, etc.) without materially departing from the novel teachings and advantages of the subject matter recited in this application. For example, elements shown as integrally formed may be constructed of multiple parts or elements, the position of elements may be reversed or otherwise varied, and the nature or number of discrete elements or positions may be altered or varied. Accordingly, all such modifications are intended to be included within the scope of this invention. The order or sequence of any process or method steps may be varied or re-sequenced according to alternative embodiments. In the claims, any means-plus-function clause is intended to cover the structures described herein as performing the recited function and not only structural equivalents but also equivalent structures. Other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments without departing from the scope of the present inventions. Therefore, the present invention is not limited to a particular embodiment, but extends to various modifications that nevertheless fall within the scope of the appended claims.
Moreover, in an effort to provide a concise description of the exemplary embodiments, all features of an actual implementation may not be described (i.e., those unrelated to the presently contemplated best mode of carrying out the invention, or those unrelated to enabling the invention).
It should be appreciated that in the development of any such actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort might be complex and time consuming, but would nevertheless be a routine undertaking of design, fabrication, and manufacture for those of ordinary skill having the benefit of this disclosure, without undue experimentation.
It should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the scope of the claims of the present invention.