CN207081739U - Immunity detection reagent - Google Patents
Immunity detection reagent Download PDFInfo
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- CN207081739U CN207081739U CN201720661567.2U CN201720661567U CN207081739U CN 207081739 U CN207081739 U CN 207081739U CN 201720661567 U CN201720661567 U CN 201720661567U CN 207081739 U CN207081739 U CN 207081739U
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Abstract
The utility model discloses a kind of immunity detection reagent, the box body (2) of (1) is renovated including top tape, the antigen-antibody land (4) separated by plunger (3), the first cleaning area (5), detection land (6), the second cleaning area (7), Binding Capacity area (8), the 3rd cleaning area (9) and detection zone (10) are sequentially provided with box body (2)；The plunger (3) is provided with plunger hole；The antigen-antibody land (4) is provided with sample treatment solution, and sample treatment solution also is provided with metal stirrer and is coated with the submicron order superparamagnetic immunomagnetic beads of the antibody that can be specifically bound with examination target thing or antigen.The utility model can integration complete each step of immune detection, be not easily introduced pollution, operate more convenient, accuracy of detection is higher.
Description
Technical field
A kind of immunity detection reagent is the utility model is related to, belongs to technical field of medical detection.
Background technology
Immune detection, i.e., qualitative, quantitative determination immune molecule and immunocyte, and analyze its clinical meaning.It is existing to exempt from
In epidemic disease detection method, sensitive, the reliable method method being widely used at present is to utilize fluorescein, isotope or enzymic-labelled antibody
Or antigen.Above-mentioned three kinds of conventional labels do not change the immunological characteristic of the latter after being connected chemically with antigen or antibody.
Due to being related to different processing in each step of immune detection, usual sample needs respectively to enter by multiple equipment
The processing of row different step, detection is more troublesome, and when carrying out different step, also needs to transfer sample sometimes to different
Carrier, this process are readily incorporated pollution, influence accuracy of detection.
Utility model content
The purpose of this utility model is, there is provided a kind of immunity detection reagent.It can integration completion immune detection
Each step, be not easily introduced pollution, operation is more convenient, and accuracy of detection is higher.
The technical solution of the utility model：Immunity detection reagent, it is characterized in：The box body renovated including top tape, box
It is sequentially provided with vivo by the antigen-antibody land of plunger separates, the first cleaning area, detection land, the second cleaning area, bottom
Thing land, the 3rd cleaning area and detection zone；The plunger is provided with plunger hole；The antigen-antibody land is provided with sample
Liquid is managed, metal stirrer is provided with sample treatment solution and be coated with can be with the antibody of examination target thing specific binding or antigen
Submicron order superparamagnetic immunomagnetic beads.
In above-mentioned immunity detection reagent, detection antibody or detection antigen, inspection can be set in the detection land
Surveying antibody or detection antigen can specifically bind with examination target thing.
In foregoing immunity detection reagent, the number of first cleaning area, the second cleaning area and the 3rd cleaning area is
One or more, each section are separated by plunger.
In foregoing immunity detection reagent, can be set in the Binding Capacity area can be with detection antibody or detection antigen
The luminous substrate or chromogenic substrate being combined.
Can be set in foregoing immunity detection reagent, in the detection zone luminous substrate can occur luminous or colour developing
The reaction solution of reaction, then it can detect antibody or antigen by detecting color signal or optical signal.
In foregoing immunity detection reagent, the plunger at one end is provided with spring, and the other end is with stretching out the push rod outside box body；
The downside of the push rod outer end is provided with slope.
In foregoing immunity detection reagent, the taper of 3~5 ° of the plunger hole band, central diameter is 3~5mm, so
Setting not only improve magnetic bead by and blocking the liquid in each section to pass freely through using capillarity；The antigen resists
Body land bottom is closing in wide at the top and narrow at the bottom, and each closing in side presss from both sides 25 °~35 ° angles with vertical direction, in order to fully crack
And magnetic bead is smoothly convergeed in plunger hole.
In foregoing immunity detection reagent, the antigen-antibody land, the first cleaning area, detection land, second
Cleaning area, Binding Capacity area, the 3rd cleaning area and detection zone are in box body from single-row lineal layout.
In foregoing immunity detection reagent, the antigen-antibody land, the first cleaning area, detection land, second
Cleaning area, Binding Capacity area, the 3rd cleaning area and detection zone are in box body from the U-shaped distribution of biserial.
In foregoing immunity detection reagent, the slack storage that band pumps out device can also be communicated with the detection zone
Area, for being pumped into other reaction solutions (such as terminate liquid, promoting luminescent solution) to detection zone in case of need.
Compared with prior art, the utility model is separated out multiple section (chambers using plunger in same kit
Body), each section can set the material needed for different detecting steps respectively, and can be with examination target thing specificity using being coated with
With reference to antibody or antigen mobile vehicle of the submicron order superparamagnetic immunomagnetic beads as examination target thing, can pass through
The plunger hole connected during use, reaction is combined into each section, therefore whole detection process can be in the case of controllable
(equipment be used cooperatively in same kit) carry out the step needed for all immune detections, it is not easy to produce secondary dirt
Dye, and operating efficiency greatly improves, and accuracy of detection also greatly improves, and it is many supporting more to solve immune detection needs
The problem of equipment.Due to having used plunger to be separated, it be able to can be turned on the utility model by simple mechanical action
Each section, (isolated according to materials such as paraffin without other processing, turn on and need to heat when assembling, heating can influence
Reagent and sample in kit, carry out influence accuracy of detection), in operation for it is more convenient, and the assembling of plunger is more
For assembling that is simple, being easy to reagent in each section.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model kit；
Fig. 2 is the structural representation of embodiment 2.
Mark in accompanying drawing for：1- is renovated, 2- box bodys, 3- plungers, 4- antigen-antibodies land, the cleaning areas of 5- first, 6-
Detection land, the cleaning areas of 7- second, 8- Binding Capacities area, the cleaning areas of 9- the 3rd, 10- detection zones, 11- spare memory areas,
12- springs.
Embodiment
The utility model is further described with reference to the accompanying drawings and examples, but is not intended as to the utility model
The foundation of limitation.
Embodiment 1.A kind of immunity detection reagent, as shown in Figure 1：1 box body 2 is renovated including top tape, in box body 2
It is sequentially provided with the antigen-antibody land 4 separated by plunger 3, the first cleaning area 5, detection land 6, the second cleaning area 7, bottom
Thing land 8, the 3rd cleaning area 9 and detection zone 10, the spare memory area 11 that band pumps out device is also communicated with detection zone 10；
The plunger 3 is provided with plunger hole；The antigen-antibody land 4 is provided with sample treatment solution, and metal is provided with sample treatment solution
Stirrer (ferromagnetic metal stirrer, by stirring and evenly mixing sample) and being coated with can specifically bind anti-with examination target thing
The submicron order superparamagnetic immunomagnetic beads of body or antigen.The antigen-antibody land 4, the first cleaning area 5, detection land 6,
Second cleaning area 7, Binding Capacity area 8, the 3rd cleaning area 9 and detection zone 10 in box body 2 from single-row lineal layout, it is described standby
Memory block 11 is arranged side by side with detection zone 10.
Detection antibody or detection antigen are provided with the detection land 6, detecting antibody or detection antigen can mark with detection
Thing specific binding.The number of first cleaning area 5, the second cleaning area 7 and the 3rd cleaning area 9 is one or more, respectively
Individual section is separated by plunger 3.Being provided with the Binding Capacity area 8 can be with detection antibody or detection antigen (such as biotin
The antibody goods antigen of mark) luminous substrate that is combined.The detection zone 10 and spare memory area 11 can be respectively equipped with
Promote luminescent solution and luminescent solution, the submicron order superparamagnetic immunomagnetic beads for combining luminous substrate enters rush luminescent solution, and lights
After liquid reinjects rush luminescent solution, the light for detection is produced.Described one end of plunger 3 is provided with spring 12, and the other end is with stretching out box body 2
Outer push rod；The downside of the push rod outer end is provided with slope.The taper of the plunger hole is 3~5 °, a diameter of 3~5mm, institute
It is closing in wide at the top and narrow at the bottom to state the bottom of antigen-antibody land 4, and each closing in side presss from both sides 25 °~35 ° angles with vertical direction.
Embodiment 2.A kind of immunity detection reagent, as shown in Figure 2：1 box body 2 is renovated including top tape, in box body 2
It is sequentially provided with the antigen-antibody land 4 separated by plunger 3, the first cleaning area 5, detection land 6, the second cleaning area 7, bottom
Thing land 8, the 3rd cleaning area 9 and detection zone 10, the spare memory area 11 that band pumps out device is also communicated with detection zone 10；
The plunger 3 is provided with plunger hole；The antigen-antibody land 4 is provided with sample treatment solution, and metal is provided with sample treatment solution
Stirrer (ferromagnetic metal stirrer, by stirring and evenly mixing sample) and being coated with can specifically bind anti-with examination target thing
The submicron order superparamagnetic immunomagnetic beads of body or antigen.Detection antibody or detection antigen, detection are provided with the detection land 6
Antibody or detection antigen can be specifically bound with examination target thing.The antigen-antibody land 4, the first cleaning area 5, detection knot
Area 6, the second cleaning area 7, Binding Capacity area 8, the 3rd cleaning area 9 and detection zone 10 are closed in box body 2 from the U-shaped distribution of biserial.
The number of first cleaning area 5, the second cleaning area 7 and the 3rd cleaning area 9 is one or more, and each section leads to
Plunger 3 is crossed to separate.The luminous substrate that can be combined with detection antibody or detection antigen is provided with the Binding Capacity area 8.Institute
Luminescent solution and luminescent solution can be respectively equipped with and promote by stating detection zone 10 and spare memory area 11, combine the sub-micron of luminous substrate
Level superparamagnetic immunomagnetic beads, which enters, promotees luminescent solution, and luminescent solution is reinjected after promoting luminescent solution, produces the light for detection.It is described
The one end of plunger 3 is provided with spring 12, and the other end is with stretching out the push rod outside box body 2；The downside of the push rod outer end is provided with slope.Institute
The taper for stating plunger hole is 3~5 °, a diameter of 3~5mm, and the bottom of antigen-antibody land 4 is closing in wide at the top and narrow at the bottom,
Each closing in side presss from both sides 25 °~35 ° angles with vertical direction.
A kind of operation principle of kit of the present utility model (by taking the luminous detection of substrate as an example)：
1. putting samples into box body 2, lid 1 is covered, then kit that box body 2 is inserted to supporting detection device accommodates
Groove, kit holding tank is interior to be provided with blend stop, and during insertion, the push rod 8 of each plunger 3 is promoted by blend stop successively so that each
Individual plunger 3 overcomes the elastic force of spring 7 to be moved, and causes plunger hole alignment separation cavity, turns on each section.
2. and then by electromagnetic coil array drive metal stirrer, sample is stirred evenly, the antigen (or antibody) in sample
It is combined with the antibody on submicron order superparamagnetic immunomagnetic beads (hereinafter referred to as carrier)；
3. carrier elutes impurity by the first cleaning area 5；
4. carrier enters detection land 6, detect on the antigen (sample) in antibody binding to carrier；
5. carrier elutes uncombined detection antibody by the second cleaning area 7；
6. carrier enters Binding Capacity area 8, luminous substrate is attached on the detection antibody on carrier；
7. carrier elutes uncombined luminous substrate by the 3rd cleaning area 9；
8. carrier enters detection zone 10, luminescent solution is pumped into detection zone 10 by spare memory area 11, the luminous substrate on carrier
Light change (luminous) is produced under the collective effect of luminescent solution and rush luminescent solution；
9. external equipment is popped one's head in using optical detection and obtains light intensity signal from the transparent window of detection detection zone, with this
Signal is qualitatively or quantitatively detected to the antigen in sample.
Note：To the antigen in detection sample, corresponding antibody, detection knot are changed on submicron order superparamagnetic immunomagnetic beads
Close in area 6 and also make corresponding antibody into.
Claims (10)
1. immunity detection reagent, it is characterised in that：The box body (2) of (1) is renovated including top tape, is sequentially provided with box body (2)
By plunger (3) separate antigen-antibody land (4), the first cleaning area (5), detection land (6), the second cleaning area (7),
Binding Capacity area (8), the 3rd cleaning area (9) and detection zone (10)；The plunger (3) is provided with plunger hole；The antigen-antibody
Land (4) is provided with sample treatment solution, and metal stirrer is provided with sample treatment solution and be coated with can be special with examination target thing
Property combine antibody or antigen submicron order superparamagnetic immunomagnetic beads.
2. immunity detection reagent according to claim 1, it is characterised in that：Inspection is provided with detection land (6)
Antibody or detection antigen are surveyed, detecting antibody or detection antigen can specifically bind with examination target thing.
3. immunity detection reagent according to claim 1, it is characterised in that：First cleaning area (5), the second cleaning
The number of area (7) and the 3rd cleaning area (9) is one or more, and each section is separated by plunger (3).
4. immunity detection reagent according to claim 2, it is characterised in that：Energy is provided with the Binding Capacity area (8)
The luminous substrate or chromogenic substrate being combined with detection antibody or detection antigen.
5. immunity detection reagent according to claim 4, it is characterised in that：Being provided with the detection zone (10) can be to hair
Luminous or chromogenic reaction reaction solution occurs for light substrate.
6. immunity detection reagent according to claim 1, it is characterised in that：Described plunger (3) one end is provided with spring
(12), the other end is with stretching out the push rod of box body (2) outside；The downside of the push rod outer end is provided with slope.
7. immunity detection reagent according to claim 1, it is characterised in that：The taper of 3~5 ° of the plunger hole band, in
Core diameter is 3~5mm, and antigen-antibody land (4) bottom is closing in wide at the top and narrow at the bottom, each closing in side and vertical direction
Press from both sides 25 °~35 ° angles.
8. immunity detection reagent according to claim 1, it is characterised in that：The antigen-antibody land (4), first
Cleaning area (5), detection land (6), the second cleaning area (7), Binding Capacity area (8), the 3rd cleaning area (9) and detection zone (10)
In box body (2) from single-row lineal layout.
9. immunity detection reagent according to claim 1, it is characterised in that：The antigen-antibody land (4), first
Cleaning area (5), detection land (6), the second cleaning area (7), Binding Capacity area (8), the 3rd cleaning area (9) and detection zone (10)
In box body (2) from the U-shaped distribution of biserial.
10. according to the immunity detection reagent described in claim 1,8 or 9, it is characterised in that：Also connect on the detection zone (10)
It is connected with the spare memory area (11) that band pumps out device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720661567.2U CN207081739U (en) | 2017-06-08 | 2017-06-08 | Immunity detection reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720661567.2U CN207081739U (en) | 2017-06-08 | 2017-06-08 | Immunity detection reagent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207081739U true CN207081739U (en) | 2018-03-09 |
Family
ID=61437105
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720661567.2U Active CN207081739U (en) | 2017-06-08 | 2017-06-08 | Immunity detection reagent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207081739U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107290524A (en) * | 2017-06-08 | 2017-10-24 | 赵怀 | A kind of immunity detection reagent |
-
2017
- 2017-06-08 CN CN201720661567.2U patent/CN207081739U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107290524A (en) * | 2017-06-08 | 2017-10-24 | 赵怀 | A kind of immunity detection reagent |
| CN107290524B (en) * | 2017-06-08 | 2024-03-19 | 杭州遂真生物技术有限公司 | Immunodetection kit |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210608 Address after: 311121 the first and second floors of building 4 and the first floor of building 3, 21 Futai Road, Zhongtai street, Yuhang District, Hangzhou City, Zhejiang Province Patentee after: HANGZHOU SUIZENG BIOTECHNOLOGY Co.,Ltd. Address before: 310000 room 108, building 1, No.16, baodaiqiaohexia, Xiacheng District, Hangzhou City, Zhejiang Province Patentee before: Zhao Huai Patentee before: Wang Qin |