CN114380629A - Composite microbial straw decomposing inoculant and preparation method thereof - Google Patents
Composite microbial straw decomposing inoculant and preparation method thereof Download PDFInfo
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- CN114380629A CN114380629A CN202210144781.6A CN202210144781A CN114380629A CN 114380629 A CN114380629 A CN 114380629A CN 202210144781 A CN202210144781 A CN 202210144781A CN 114380629 A CN114380629 A CN 114380629A
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- 239000010902 straw Substances 0.000 title claims abstract description 33
- 230000000813 microbial effect Effects 0.000 title claims abstract description 13
- 239000002131 composite material Substances 0.000 title claims abstract description 9
- 239000002054 inoculum Substances 0.000 title claims description 7
- 238000002360 preparation method Methods 0.000 title abstract description 5
- 241000985513 Penicillium oxalicum Species 0.000 claims abstract description 31
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 26
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 20
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000004721 Polyphenylene oxide Substances 0.000 claims abstract description 19
- 235000013312 flour Nutrition 0.000 claims abstract description 19
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims abstract description 19
- 229940107187 fructooligosaccharide Drugs 0.000 claims abstract description 19
- 229920000570 polyether Polymers 0.000 claims abstract description 19
- 238000000354 decomposition reaction Methods 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims description 31
- 230000004151 fermentation Effects 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 24
- 239000002245 particle Substances 0.000 claims description 15
- 150000001875 compounds Chemical class 0.000 claims description 14
- 239000008188 pellet Substances 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 11
- 238000000151 deposition Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 241000202921 Ureaplasma urealyticum Species 0.000 claims description 2
- 238000013329 compounding Methods 0.000 claims description 2
- 239000002361 compost Substances 0.000 abstract description 15
- 239000003674 animal food additive Substances 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000009264 composting Methods 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 235000015278 beef Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000609240 Ambelania acida Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to the field of compost, and in particular relates to a composite microbial straw decomposing agent and a preparation method thereof. Further, the feed additive is prepared from the following raw materials in parts by weight: 20-30 parts of CM complex microbial inoculum, 1-3 parts of fructo-oligosaccharide, 1-6 parts of penicillium oxalicum HB 13, 6-423 parts of Bacillus urealyticum strain TB 423, 5-10 parts of malt flour and 0.03-0.06 part of polyether. The invention can realize the rapid decomposition of the straws, and the obtained compost can promote the growth of crops and improve the quality of the crops, thereby achieving the purpose of increasing the yield.
Description
Technical Field
The invention relates to the field of compost, and particularly relates to a composite microbial straw decomposing inoculant and a preparation method thereof.
Background
At present, most of the straws in China are used for returning to the field, so that the soil quality is improved, and the crop yield is increased. Because the crop straws mainly comprise cellulose, hemicellulose and lignin, are slowly decomposed and are difficult to decompose by microorganisms in the natural environment, the active ingredients of the crop straws are rarely utilized in the season, and the physical properties of soil and field cultivation are influenced, so that the returning utilization of the important organic matter resource of the straws is limited. Therefore, the method for exploring the straw biological decomposition agent can quickly decompose the crop straws, improve the operability of straw returning or apply the straw compost to farmlands after preparing the organic fertilizer, and has very important practical significance for promoting the sustainable development of agriculture. However, most of the existing biological straw decomposing agents are prepared from filter mud, cassava residues and bagasse ash, and have the defects that after the decomposing agents are added into the straws, microorganisms cannot be reactivated quickly, colonize, the activity of the microorganisms for generating enzyme is low, and the straws are not easy to decompose quickly, and the improvement is needed.
Disclosure of Invention
In order to solve the problems, the invention provides a composite microbial straw decomposition agent which can realize the rapid decomposition of straws.
In order to achieve the purpose, the invention adopts the technical scheme that:
a composite microbial straw decomposing inoculant is prepared by compounding CM complex inoculant, fructo-oligosaccharide, penicillium oxalicum HB1, Bacillus urealyticum strain TB42, malt flour and polyether.
Further, the feed additive is prepared from the following raw materials in parts by weight:
20-30 parts of CM complex microbial inoculum, 1-3 parts of fructo-oligosaccharide, 1-6 parts of penicillium oxalicum HB 13, 6-423 parts of Bacillus urealyticum strain TB 423, 5-10 parts of malt flour and 0.03-0.06 part of polyether.
Preferably, the feed additive is prepared from the following raw materials in parts by weight:
20 parts of CM complex microbial inoculum, 1 part of fructo-oligosaccharide, 13 parts of penicillium oxalicum HB, 423 parts of Bacillus urealyticum strain TB, 5 parts of malt flour and 0.03 part of polyether.
Preferably, the feed additive is prepared from the following raw materials in parts by weight:
30 parts of CM complex microbial inoculum, 3 parts of fructo-oligosaccharide, 16 parts of penicillium oxalicum HB, 426 parts of Bacillus urealyticum strain TB, 10 parts of malt flour and 0.06 part of polyether.
Preferably, the feed additive is prepared from the following raw materials in parts by weight:
25 parts of CM complex microbial inoculum, 2 parts of fructo-oligosaccharide, 14.5 parts of penicillium oxalicum HB, 424.5 parts of Bacillus urealyticum strain TB, 7.5 parts of malt flour and 0.045 part of polyether.
The invention also provides a preparation method of the composite microbial straw decomposing inoculant, which comprises the following steps:
s1, obtaining a penicillium oxalicum HB1 fermentation liquid and a bacillus urealyticum strain TB42 fermentation liquid, wherein the number of live bacteria in the penicillium oxalicum HB1 fermentation liquid is more than or equal to 1 multiplied by 105Per ml; the number of viable bacteria in the fermentation liquor of the ureaplasma urealyticum strain TB42 is more than or equal to 1 multiplied by 108Per ml;
s2, mixing and stirring the fructo-oligosaccharide, the malt flour and the polyether uniformly, and granulating to obtain particles with the particle size of about 20 meshes;
s3, depositing the CM compound microbial inoculum on the surface of the deposited particles by adopting a powder deposition method to obtain a bacterium-carrying pellet;
s4, independently packaging the penicillium oxalicum HB1 fermentation liquid, the bacillus urealyticum TB42 fermentation liquid and the bacteria-carrying pellets according to the components to obtain the compound.
The invention has the following beneficial effects:
the straw can be quickly decomposed, and meanwhile, the obtained compost can promote the growth of crops and improve the quality of the crops, so that the purpose of increasing the yield is achieved.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
S1, weighing the following components in parts by weight: 20 parts of CM complex microbial inoculum, 1 part of fructo-oligosaccharide, 13 parts of penicillium oxalicum HB, 423 parts of Bacillus urealyticum strain TB, 5 parts of malt flour and 0.03 part of polyether
S2, mixing corn straw dry powder: beef extract peptone: the solution of the penicillium oxalicum HB1 is prepared from the following raw materials in parts by weight of 11 g: 1.5 g: mixing 1mL of the mixture, adding 100mL of water, fermenting at about 30 ℃, wherein the initial pH value is 5, the rotating speed of a shaking table is 190r/min, the fermentation time is 80h, and the number of the obtained viable bacteria is more than or equal to 1 multiplied by 105The fermentation liquor of the penicillium oxalicum HB1 is one/ml;
inoculating Bacillus urealyticum strain TB42 liquid into 1L sterilized LB culture solution, culturing in a shaker at 50 deg.C and 120rpm for 24 hr to obtain fermentation liquid with viable bacteria number not less than 1 × 108Bacillus ureafaciens strain TB42 per ml;
s3, mixing and stirring the fructo-oligosaccharide, the malt flour and the polyether uniformly, and granulating to obtain particles with the particle size of about 20 meshes;
s4, depositing the CM compound microbial inoculum on the surface of the deposited particles by adopting a powder deposition method to obtain a bacterium-carrying pellet;
s5, independently packaging the penicillium oxalicum HB1 fermentation liquid, the bacillus urealyticum TB42 fermentation liquid and the bacteria-carrying pellets according to the components to obtain the compound.
Example 2
S1, weighing the following components in parts by weight: 30 parts of CM complex microbial inoculum, 3 parts of fructo-oligosaccharide, 16 parts of penicillium oxalicum HB, 426 parts of Bacillus urealyticum strain TB, 10 parts of malt flour and 0.06 part of polyether;
s2, mixingCorn straw dry powder: beef extract peptone: the solution of the penicillium oxalicum HB1 is prepared from the following raw materials in parts by weight of 11 g: 1.5 g: mixing 1mL of the mixture, adding 100mL of water, fermenting at about 30 ℃, wherein the initial pH value is 5, the rotating speed of a shaking table is 190r/min, the fermentation time is 80h, and the number of the obtained viable bacteria is more than or equal to 1 multiplied by 105The fermentation liquor of the penicillium oxalicum HB1 is one/ml;
inoculating Bacillus urealyticum strain TB42 liquid into 1L sterilized LB culture solution, culturing in a shaker at 50 deg.C and 120rpm for 24 hr to obtain fermentation liquid with viable bacteria number not less than 1 × 108Bacillus ureafaciens strain TB42 per ml;
s3, mixing and stirring the fructo-oligosaccharide, the malt flour and the polyether uniformly, and granulating to obtain particles with the particle size of about 20 meshes;
s4, depositing the CM compound microbial inoculum on the surface of the deposited particles by adopting a powder deposition method to obtain a bacterium-carrying pellet;
s5, independently packaging the penicillium oxalicum HB1 fermentation liquid, the bacillus urealyticum TB42 fermentation liquid and the bacteria-carrying pellets according to the components to obtain the compound.
Example 3
S1, weighing the following components in parts by weight: 25 parts of CM complex microbial inoculum, 2 parts of fructo-oligosaccharide, 14.5 parts of penicillium oxalicum HB, 424.5 parts of Bacillus urealyticum strain TB, 7.5 parts of malt flour and 0.045 part of polyether;
s2, mixing corn straw dry powder: beef extract peptone: the solution of the penicillium oxalicum HB1 is prepared from the following raw materials in parts by weight of 11 g: 1.5 g: mixing 1mL of the mixture, adding 100mL of water, fermenting at about 30 ℃, wherein the initial pH value is 5, the rotating speed of a shaking table is 190r/min, the fermentation time is 80h, and the number of the obtained viable bacteria is more than or equal to 1 multiplied by 105The fermentation liquor of the penicillium oxalicum HB1 is one/ml;
inoculating Bacillus urealyticum strain TB42 liquid into 1L sterilized LB culture solution, culturing in a shaker at 50 deg.C and 120rpm for 24 hr to obtain fermentation liquid with viable bacteria number not less than 1 × 108Bacillus ureafaciens strain TB42 per ml;
s3, mixing and stirring the fructo-oligosaccharide, the malt flour and the polyether uniformly, and granulating to obtain particles with the particle size of about 20 meshes;
s4, depositing the CM compound microbial inoculum on the surface of the deposited particles by adopting a powder deposition method to obtain a bacterium-carrying pellet;
s5, independently packaging the penicillium oxalicum HB1 fermentation liquid, the bacillus urealyticum TB42 fermentation liquid and the bacteria-carrying pellets according to the components to obtain the compound.
When the special bacteria pellet is used, the penicillium oxalicum HB1 fermentation liquid, the bacillus ureafaciens strain TB42 fermentation liquid and the bacteria-carrying pellet are mixed and stirred until the bacteria-carrying pellet is completely dispersed.
Compost test
The straw decomposition agent obtained in example 3 was added to prepare an experimental group, the CM complex microbial inoculum was added in an amount equivalent to that in example 3 to prepare control group 1, the fermentation broth of bacillus ureafaciens strain TB42 was added in an amount equivalent to that in example 3 to prepare control group 2, and the fermentation broth of penicillium oxalicum HB1 was added in an amount equivalent to that in example 3 to prepare control group 3.
The compost raw materials are cow dung and corn straws, and the mass ratio is 17:5, and the total mass ratio is 20 kg. And (4) composting for 45d, sampling at 0 th, 5 th, 10 th, 15 th, 25 th, 30 th and 45 th d, and measuring parameters such as temperature, pH, water content, organic matters, Kjeldahl nitrogen, inorganic nitrogen, DOM content and the like. The microbial inoculum is inoculated twice in the whole composting process, wherein the inoculation time is 0d and 10d respectively. The stack was turned three times for 10d, 20d and 30d respectively.
Results of the experiment
From the temperature of the composting process, the temperature of the experimental group is obviously increased compared with that of the control group 1, the control group 2 and the control group 3, so that the killing of pathogens in the compost is facilitated, the high-temperature degradation of compost materials is accelerated, and the decomposition of the compost is promoted.
Compared with a control group 1, a control group 2 and a control group 3, the pH value of the compost of the experimental group is increased to be higher, and the moisture content is reduced to be faster, namely the reaction of the pile of the experimental group is more violent, so that the NH3 is volatilized greatly to increase the pH value, and the heat generation is more, so that a large amount of moisture is taken away.
From the viewpoint of the change of the content of the heap soluble organic matter (DOM), the DOM content of the experimental group is reduced from 69.8mg/g to 3.7mg/g, which is reduced by 94.7%.
In conclusion, the straw decomposition agent can increase the composting temperature, accelerate the water loss, accelerate the degradation of organic matters and DOM and promote the compost decomposition.
And (3) field test:
applying compost obtained from experimental group, control group 1, control group 2 and control group 3 to wheat planting, and selectingFour blocks of 6m × 5m =30m are taken2The compost obtained by the experimental group is applied to the experimental field No. 1, and the compost obtained by the control group 1, the control group 2 and the control group 3 is applied to the experimental field No. 2-4, wherein the application modes and the application amounts of the four groups are completely the same, and the treatment of the rest fields has no obvious difference. As a result, it was found that: the wheat yield of the experimental group 1 is significantly higher than that of the control group 1, the control group 2 and the control group 3, and is increased by 11%, 17% and 20% respectively.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (6)
1. A composite microbial straw decomposing inoculant is characterized in that: is prepared by compounding CM complex microbial inoculum, fructo-oligosaccharide, penicillium oxalicum HB1, Bacillus urealyticum strain TB42, malt flour and polyether.
2. The compound microbial straw decomposition agent of claim 1, wherein: the feed is prepared from the following raw materials in parts by weight: 20-30 parts of CM complex microbial inoculum, 1-3 parts of fructo-oligosaccharide, 1-6 parts of penicillium oxalicum HB 13, 6-423 parts of Bacillus urealyticum strain TB 423, 5-10 parts of malt flour and 0.03-0.06 part of polyether.
3. The compound microbial straw decomposition agent of claim 1, wherein: the feed is prepared from the following raw materials in parts by weight: 20 parts of CM complex microbial inoculum, 1 part of fructo-oligosaccharide, 13 parts of penicillium oxalicum HB, 423 parts of Bacillus urealyticum strain TB, 5 parts of malt flour and 0.03 part of polyether.
4. The compound microbial straw decomposition agent of claim 1, wherein: the feed is prepared from the following raw materials in parts by weight: 30 parts of CM complex microbial inoculum, 3 parts of fructo-oligosaccharide, 16 parts of penicillium oxalicum HB, 426 parts of Bacillus urealyticum strain TB, 10 parts of malt flour and 0.06 part of polyether.
5. The compound microbial straw decomposition agent of claim 1, wherein: the feed is prepared from the following raw materials in parts by weight: 25 parts of CM complex microbial inoculum, 2 parts of fructo-oligosaccharide, 14.5 parts of penicillium oxalicum HB, 424.5 parts of Bacillus urealyticum strain TB, 7.5 parts of malt flour and 0.045 part of polyether.
6. The method for preparing the composite microbial straw decomposition agent as claimed in any one of claims 1 to 5, wherein: the method comprises the following steps:
s1, obtaining a penicillium oxalicum HB1 fermentation liquid and a bacillus urealyticum strain TB42 fermentation liquid, wherein the number of live bacteria in the penicillium oxalicum HB1 fermentation liquid is more than or equal to 1 multiplied by 105Per ml; the number of viable bacteria in the fermentation liquor of the ureaplasma urealyticum strain TB42 is more than or equal to 1 multiplied by 108Per ml;
s2, mixing and stirring the fructo-oligosaccharide, the malt flour and the polyether uniformly, and granulating to obtain particles with the particle size of about 20 meshes;
s3, depositing the CM compound microbial inoculum on the surface of the deposited particles by adopting a powder deposition method to obtain a bacterium-carrying pellet;
s4, independently packaging the penicillium oxalicum HB1 fermentation liquid, the bacillus urealyticum TB42 fermentation liquid and the bacteria-carrying pellets according to the components to obtain the compound.
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| CN115418335A (en) * | 2022-09-30 | 2022-12-02 | 武汉工程大学 | Composite microbial agent for high-temperature composting of straws and preparation method thereof |
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