CN114377153A - 以碳量子点为核的金属有机框架复合材料作为药物载体的制备与应用 - Google Patents

以碳量子点为核的金属有机框架复合材料作为药物载体的制备与应用 Download PDF

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CN114377153A
CN114377153A CN202210045917.8A CN202210045917A CN114377153A CN 114377153 A CN114377153 A CN 114377153A CN 202210045917 A CN202210045917 A CN 202210045917A CN 114377153 A CN114377153 A CN 114377153A
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孙艳
李育霖
解永超
杨迪
张赫
叶凌涛
王羽
刘献斌
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Heilongjiang Bayi Agricultural University
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Abstract

以碳量子点为核的金属有机框架复合材料作为药物载体的制备与应用。本发明属于纳米生物材料技术领域，具体涉及以碳量子点为核的金属有机框架纳米材料的制备方法以及作为探针和基因药物载体的应用。首先，制备1～10nm的碳量子点；在碳量子点表面修饰以金属有机框架中有机连接桥的类似物；然后将修饰后的碳量子点悬浮于合成金属有机框的有机介质中；在有机介质中形成以碳量子点为晶核的金属有机框架多孔材料。本发明将上述合成的以碳量子点为核的金属有机框架纳米材料作为纳米探针和纳米基团药物载体应用于生物学和药学领域。

Description

以碳量子点为核的金属有机框架复合材料作为药物载体的制 备与应用
技术领域
本发明属于纳米生物材料技术领域，具体涉及以碳量子点为核的金属有机框架的纳米材料的制备方法以及作为探针和基因药物载体的应用。
背景技术
金属有机框架是一种金属离子与有机连接体桥接而形成的纳米多孔材料。因其具有清晰的结构、孔隙率高、比表面积极高、密度低以及生物相容性强等诸多优点，被认为是一类极富潜力的新型纳米药物载体，近年来被广泛应用于生物医学研究领域。但是通常认为金属有机框架在体内的毒性要高于纳米二氧化硅、碳酸钙和磷酸钙等纳米载体。因此金属有机框架从进入体内到完全从体内排出的全过程就需要进行系统的研究和监测。
目前大多数的研究都是把化学荧光素等物质负载在金属有机框架表面或者其孔道中。在药物的转运过程中，无法做到零释放，会对整个荧光监控过程产生干扰。在到达特定环境时，随着药物释放化学荧光会被释放从而消散或猝灭。碳量子点(CDs)是碳纳米材料家族的新秀，一般小于10nm。碳量子点具有良好的荧光特性，不仅表现在光稳定性强、抗光漂白、无光闪烁现象以及发射波长可调等，还具有低密度、良好的生物相容性等优点。
发明内容
本发明的第一个目的是设计以碳量子点为核的金属有机框架复合材料；本发明的第二个目的是提供上述复合材料的制备方法；本发明的第三个目的是提供上述复合材料的应用，主要应用方向为新型的纳米探针和药物载体。
为了实现上述的第一个目的，本发明进行了以下的设计：以碳量子点为晶核，在碳量子点上生长金属有机框架结构，形成以碳量子点为核的金属有机框架复合材料。
对上述设计做出进一步说明如下：
所述的以碳量子点为核的金属有机框架复合材料，其作为晶核的碳量子点，尺寸在1～10nm之间；
所述的以碳量子点为核的金属有机框架复合材料，在紫外光激发下，其荧光的颜色包括但不限于绿色，蓝色和红色等；
所述的以碳量子点为核的金属有机框架复合材料，其形成的金属有机框架，包括但不限于ZIF系列、MOF系列、MIL系列等。
为了实现上述的第二个目的，本发明采用了以下的技术方案：首先，制备1～10nm的碳量子点；在碳量子点表面修饰以金属有机框架中有机连接桥的类似物；然后将修饰后的碳量子点悬浮于合成金属有机框的有机介质中；在有机介质中形成以碳量子点为晶核的金属有机框架多孔材料。
对上述技术方案做出进一步说明如下：
所述的以碳量子点为核的金属有机框架复合材料，其碳量子点的合成方法包括但不限于水热合成法、微波法和模板法等；
所述的以碳量子点为核的金属有机框架复合材料，碳量子点表面进行金属有机框架中有机连接桥类似物的修饰；
所述的以碳量子点为核的金属有机框架复合材料，其碳量子点的合成介质或悬浮介质主要根据欲合成的金属有机框架确定，与欲合成的金属有机框架的合成介质一致；在该有机介质中形成以碳量子点为晶核的金属有机框架多孔材料；
所述的以碳量子点为核的金属有机框架复合材料，其金属有机框架的尺寸介于10～250nm之间。
为了实现上述的第三个目的，本发明进行了以下的设计：将上述的以碳量子点为核的金属有机框架的复合材料，作为新型的纳米基因药物载体，负载基因或药物到其表面和孔道中。利用碳量子点的荧光特性可追踪载体转运的全过程、载体部分分解以及排出体外的过程。对上述的以碳量子点为核的金属有机框架复合材料，可以进行靶向剂修饰、细胞穿透剂修饰，门控修饰等各种纳米基团药物载体的修饰，其荧光特性完全不影响后续的载体功能和基因与药物的负载、转运与控释。
附图说明
图1是以碳量子点为核的金属有机框架合成示意图。其中，a为量子点，b为表面修饰以金属有机框架连接桥类似物的碳量子点，c为以碳量子点为核的金属有机框架，d为负载上药物和小核酸的以碳量子点为核的金属有机框架。
具体实施方式
实施例一：以碳量子点为核的金属有机框架ZIF-8(CDs@ZIF-8)复合材料的制备
1)碳量子点溶液的制备：①蔗糖溶液配制：以甲醇为介质，取蔗糖溶于其中，置于磁力搅拌器上搅拌至无明显颗粒，即搅拌均匀备用。②硫酸溶液配制：以甲醇为介质，取适量浓硫酸溶于其中，然后置于磁力搅拌器上搅拌，混合均匀留以备用。③取适量先前配制好的硫酸溶液混合在配制好的蔗糖溶液中，混合搅拌均匀。④将搅拌后的蔗糖溶液装入不锈钢高温反应釜，再转入事先预热到180℃的烘箱加热6小时后，得到碳量子点溶液。⑤碳量子点表面修饰以2-甲基咪唑类似物，得到修饰后碳量子点的溶液。
2)CDs@ZIF-8的制备：①取0.3g的六水合硝酸锌溶解在上述修饰后碳量子点的甲醇溶液中，置于磁力搅拌器上搅拌均匀留以待用。②再称取0.66g的2-甲基咪唑溶解在上述修饰后碳量子点的甲醇溶液中，置于磁力搅拌器上搅拌均匀留以待用。③将以上溶液混合后，置于磁力搅拌器上剧烈搅拌。④搅拌结束后，将溶液转至不锈钢高温反应釜后放入事先预热至150℃的烘箱中加热5小时。⑤加热结束后，取出冷却，然后用高速离心机清洗，每次离心时间为5min。先甲醇清洗三次，再用乙醇清洗三次，最后重悬在乙醇中过夜干燥，得到CDs@ZIF-8成品。
实施例二：以碳量子点为核的氨基MIL-53(Al)的制备CDs@NH2-MIL-53(Al)的制备1)碳量子点溶液的制备：①取L-半胱氨酸和蔗糖于圆底烧瓶中，并加入氢氧化钠溶液。②在80℃下，将上述混合液过夜回流反应，得到橙黄色液体。待其冷却至室温后，将所得到的溶液离心(13000rpm，15min)除去较大颗粒。③将最终得到的碳点溶液透析两天(透析袋截留分子量为1000Da)，收集透析袋的内液并旋蒸至深黄色的液体。④冷冻干燥得到纯的碳量子点粉末，将其表面修饰以对苯二甲酸类似物，在N，N-二甲基甲酰胺中悬浮配制成碳量子点储备液，置于4℃的冰箱备用。
2)CDs@NH2-MIL-53(Al)的制备：①取九水合硝酸铝和2-氨基对苯二甲酸溶解在碳量子点储备液中。②将上述溶液转移到提前预热到130℃的不锈钢反应釜中，反应72小时。③待冷却后，得到黄色固体，离心分离，先用丙酮洗三次，再用甲醇洗三次。④将洗涤过后的产物，悬浮在甲醇中，重新转入到不锈钢反应釜中，过夜活化。⑤将活化后的产物再用甲醇洗涤1-2次，80℃干燥过夜。最终形成以碳量子点为核的氨基MIL-53(Al)(CDs@NH2-MIL-53(Al))。
实施例三：CDs@ZIF-8@siRNA@转铁蛋白作为基因载体的应用
将具体实施方案1中的制备的CDs@ZIF-8材料，通过浴式吸附的方式，使其携带有小干扰RNA(siRNA)，形成CDs@ZIF-8@siRNA。具体过程如下，取0.2mg的CDs@ZIF-8纳米颗粒，置于20μL的siRNA溶液中，避光静置6小时，形成CDs@ZIF-8@siRNA。将上述纳米颗粒置于0.2mg/mL的转铁蛋白20μL中，避光翻转4小时，形成CDs@ZIF-8@siRNA@转铁蛋白。9000rpm离心，然后用灭菌超纯水洗2次，加入1mL的无血清培养液重悬；在孔板中每孔中加入500μL的重悬材料与细胞共培养，4小时后换含血清培养液进行培养，研究载体的细胞摄入、转染效率与细胞凋亡。

Claims (10)

1.一种采用以碳量子点为晶核，在碳量子点上生长金属有机框架结构，形成以碳量子点为核的金属有机框架的复合材料。
2.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，其作为晶核的碳量子点，尺寸在1～10nm之间。
3.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，在紫外光激发下，其荧光的颜色包括但不限于绿色，蓝色和红色等。
4.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，其形成的金属有机框架，包括但不限于ZIF系列、MOF系列、MIL系列等。
5.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，碳量子点表面进行金属有机框架中有机连接桥类似物的修饰。
6.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，其碳量子点的合成介质或悬浮介质主要根据欲合成的金属有机框架确定，与欲合成的金属有机框架的合成介质一致；在该有机介质中形成以碳量子点为晶核的金属有机框架多孔材料。
7.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，其金属有机框架的尺寸介于10～250nm之间。
8.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，其特性是利用碳量子点的荧光特性可追踪载体转运的全过程和载体部分分解以及排出体外的过程。
9.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，可以进行但不限于靶向剂修饰、细胞穿透剂修饰，门控修饰等各种纳米基团药物载体的修饰。
10.根据权利要求书1所述的以碳量子点为核的金属有机框架的复合材料，其碳量子点的荧光特性完全不影响后续的载体功能和基因与药物的负载、转运与控释。
CN202210045917.8A 2022-01-06 2022-01-06 以碳量子点为核的金属有机框架复合材料作为药物载体的制备与应用 Pending CN114377153A (zh)

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CN113512423A (zh) * 2021-07-17 2021-10-19 重庆医科大学 一种基于金属有机框架的荧光碳量子点及其制备方法

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CN117534883A (zh) * 2024-01-10 2024-02-09 广州双一乳胶制品有限公司 一种抗静电乳胶手套及其制备方法
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