CN114375767A - Culture medium for culturing phellinus igniarius, and method and application for culturing phellinus igniarius - Google Patents
Culture medium for culturing phellinus igniarius, and method and application for culturing phellinus igniarius Download PDFInfo
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- CN114375767A CN114375767A CN202210026942.1A CN202210026942A CN114375767A CN 114375767 A CN114375767 A CN 114375767A CN 202210026942 A CN202210026942 A CN 202210026942A CN 114375767 A CN114375767 A CN 114375767A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention relates to a culture medium for culturing phellinus igniarius, a method for culturing phellinus igniarius and application, and belongs to the technical field of phellinus igniarius culture. The invention provides a culture medium for culturing phellinus igniarius, which is prepared from raw materials including corn straw powder and water. The culture medium disclosed by the invention can realize large-scale culture of phellinus igniarius and waste utilization of corn straws, and the culture medium is more abundant and cheap, and is more convenient in culture conditions and suitable for large-scale culture.
Description
Technical Field
The invention relates to the technical field of phellinus igniarius culture, and in particular relates to a culture medium for phellinus igniarius culture, a method for culturing phellinus igniarius and application of the culture medium.
Background
Phellinus linteus is a medicinal fungus, and wild Phellinus linteus is grown on trunks of Morus alba, Tilia Miqueliana Maxim and the like. With the intensive research on phellinus igniarius in recent years, the phellinus igniarius polysaccharide is found to have obvious effects of resisting tumors and inflammation, and the polyphenol has the effects of resisting oxidation, protecting lungs and the like. The medicinal value of Phellinus linteus is gradually discovered. However, the wild phellinus igniarius is picked in large scale, the quantity of the phellinus igniarius cannot meet the market demand, and the phellinus igniarius sporocarp grows for many years, grows slowly and is difficult to cultivate. In the artificial culture process, the wild survival conditions of the phellinus igniarius are mainly simulated for large-scale culture, a large amount of wood needs to be consumed in the culture process, and the culture cost is high.
Disclosure of Invention
The invention aims to provide a culture medium for culturing phellinus igniarius, a method for culturing phellinus igniarius and application. The culture medium disclosed by the invention can realize the large-scale culture of phellinus igniarius and the waste utilization of corn straws, is more abundant and cheap in culture medium material, is more convenient in culture conditions, and is suitable for large-scale culture.
The invention provides a culture medium for culturing phellinus igniarius, which is prepared from raw materials including corn straw powder and water.
Preferably, the preparation method of the corn straw powder comprises the following steps: crushing and sieving the corn straws.
Preferably, the sieving condition is 100 mesh sieving.
Preferably, in the culture medium, the mass ratio of the corn straw powder to the water is (0.1-2.6): 100.
the invention also provides a preparation method of the culture medium in the technical scheme, which comprises the following steps:
mixing the corn straw powder and water to obtain the culture medium for culturing the phellinus igniarius.
The invention also provides a culture medium for culturing phellinus igniarius, and raw materials for preparing the culture medium comprise potatoes, sugar, corn straw powder and water in the culture medium according to the technical scheme.
Preferably, the raw materials for preparing the culture medium comprise the following components in parts by weight: 0.1-2.6 parts of corn straw powder, 0.1-0.6 part of potato, 0-2 parts of sugar and 100 parts of water.
The invention also provides a preparation method of the culture medium in the technical scheme, which comprises the following steps:
preparing a potato basal culture medium by taking potatoes and sugar as raw materials; and mixing the potato basal culture medium, the corn straw powder and water to obtain the culture medium for culturing the phellinus igniarius.
The invention also provides a method for liquid culture of phellinus igniarius, which comprises the steps of inoculating phellinus igniarius into the culture medium of the technical scheme for culture to obtain phellinus igniarius; the temperature of the culture is 25-37 ℃, and the pH value of the culture is 4-8.
The invention also provides application of the corn straw in culturing phellinus igniarius.
The invention provides a culture medium for culturing phellinus igniarius. The invention optimizes the culture medium of phellinus igniarius, treats the corn straws into corn straw powder as a phellinus igniarius carbon source, and has higher yield than a potato basal culture medium after liquid culture. The raw materials of the preparation method are agricultural waste straws, and the raw materials are wide and sufficient in source, cheap and easy to obtain, and low in production cost. Compared with other culture media, the culture medium has the advantages of greatly reducing energy consumption of raw materials, improving simplicity and convenience in operation and rapidness, along with low input price and relatively high yield. The invention opens up a new way for the treatment of crop straws, fully embodies the economy while solving the environmental problem, increases the new way of resource utilization, promotes the recycling of agricultural waste resources, brings great economic benefit and has great ecological benefit and social benefit.
Drawings
FIG. 1 is a graph showing the results of culturing the dry powders of the straw carbon powder and the potato-based solid medium according to the present invention in different mixing ratios, as provided in comparative example 1; wherein, A is pictures No. 1, No. 2, No. 3 and No. 10 when the culture is carried out for 5 days, and B is pictures No. 1-9 when the culture is carried out for 16 days;
fig. 2 is a graph of the culture results of different mixing ratios of the dry powder and the corn straw powder of the potato basic liquid culture medium provided in embodiment 1 of the present invention.
Detailed Description
The invention provides a culture medium for culturing phellinus igniarius, which is prepared from raw materials including corn straw powder and water. In the present invention, the method for preparing the corn straw powder preferably comprises the following steps: crushing and sieving the corn straws. The present invention preferably further comprises the operation of washing the corn stover prior to said crushing. In the present invention, it is preferable to perform drying after the washing, and then perform crushing after the drying. The method for crushing is not particularly limited in the present invention, and conventional crushing methods known to those skilled in the art, such as crushing of corn stalks using a centrifugal crusher, may be used. After the crushing, the invention carries out sieving, and in the invention, the sieving condition is preferably 100-mesh sieving. In the invention, in the culture medium, the mass ratio of the corn straw powder to the water is preferably (0.1-2.6): 100, more preferably 2.6g of the corn straw powder and 100ml of water, and the method has higher yield and highest economic benefit. In the invention, the pH of the culture medium is preferably 4-8, and more preferably 6.
The invention also provides a preparation method of the culture medium in the technical scheme, which comprises the following steps:
mixing the corn straw powder and water to obtain the culture medium for culturing the phellinus igniarius. After the culture medium for phellinus igniarius culture is obtained, the culture medium is preferably sterilized, and the sterilization condition is preferably sterilization at 121 ℃ for 20-30 min. The culture medium prepared by the preparation method can provide a more stable growth environment for the growth of phellinus igniarius, provide sufficient nutrients for the culture of phellinus igniarius, and can culture phellinus igniarius quickly and in high quality.
The invention also provides a culture medium for culturing phellinus igniarius, and raw materials for preparing the culture medium comprise potatoes, sugar, corn straw powder and water in the culture medium according to the technical scheme. In the present invention, the raw material for preparing the culture medium preferably comprises the following components in parts by weight: 0.1-2.6 parts of corn straw powder, 0.1-0.6 part of potato, 0-2 parts of sugar and 100 parts of water. In the invention, the pH of the culture medium is preferably 4-8, and more preferably 6.
The invention also provides a preparation method of the culture medium in the technical scheme, which comprises the following steps:
preparing a potato basal culture medium by taking potatoes and sugar as raw materials; and mixing the potato basal culture medium, the corn straw powder and water to obtain the culture medium for culturing the phellinus igniarius. According to the invention, the potato basal culture medium is preferably prepared by utilizing potatoes and sugar, and is preferably preserved in a dry powder form after being prepared. In the subsequent preparation, the dry powder of the potato basic culture medium, the corn straw powder and water are preferably mixed to prepare the culture medium for phellinus igniarius culture. When the potato basal medium is stored in a dry powder form, the mixing mass ratio of the potato basal medium to the corn straw powder and the water is preferably (0.1-2.6): (0.1-2.6): 100. after the culture medium for phellinus igniarius culture is obtained, the culture medium is preferably sterilized, and the sterilization condition is preferably sterilization at 121 ℃ for 20-30 min.
The invention also provides a method for liquid culture of phellinus igniarius, which comprises the steps of inoculating phellinus igniarius into the culture medium of the technical scheme for culture to obtain phellinus igniarius; the temperature of the culture is 25-37 ℃, and the pH value of the culture is 4-8. In the present invention, the temperature of the culture is preferably 30 ℃ and the pH of the culture is preferably 6. In the present invention, the cultivation is preferably carried out in an incubator.
The invention also provides application of the corn straw in culturing phellinus igniarius. In the process of culturing phellinus linteus, phellinus linteus is mostly cultured on a large scale in a potato culture medium, and substances such as glucose and the like are used as a carbon source, or wood chips are used as a carbon source and an attachment site. The invention utilizes the corn straws as the carbon source, develops the new application of the corn straws and selects the concentration of the corn straws which is most suitable for the growth of the phellinus linteus. Compared with the traditional culture method, the operation of the phellinus igniarius culture medium prepared from the corn straws is simpler, more convenient and faster. The culture medium prepared by the preparation method can provide a more stable growth environment for the growth of phellinus igniarius, provide sufficient nutrients for the culture of phellinus igniarius, and can culture phellinus igniarius quickly and in high quality; the raw materials of the preparation method are agricultural waste straws, and the raw materials are wide and sufficient in source, cheap and easy to obtain, and low in production cost. Meanwhile, the invention opens up a new way for the treatment of crop straws, fully embodies the economy while solving the environmental problem, increases the new way of resource utilization, promotes the recycling of agricultural waste resources, brings great economic benefit and has great ecological benefit and social benefit.
The culture medium for Phellinus linteus culture, the method for culturing Phellinus linteus and the application thereof according to the present invention will be described in further detail with reference to the following specific examples, but the technical scheme of the present invention includes, but is not limited to, the following examples.
Example 1
Culturing Phellinus igniarius strains and selecting the optimal culture conditions, culturing Phellinus igniarius strains by using potato basal medium (PDA), and culturing at different temperatures and different pH values, wherein the selection temperature range is 25-37 ℃, and the pH range is 4-8. The test result shows that the optimum growth temperature of the phellinus igniarius is 30 ℃, the optimum pH value is 6, and under the condition, the phellinus igniarius strain grows fastest and the yield is higher.
Comparative example 1
Pretreatment of corn straws: mixing the corn straw with water (10: 3) under high pressure and high temperature by an autoclave, treating for 24h, and centrifuging to obtain straw precipitate.
And cleaning the precipitate, and drying to obtain the corn carbonized straw carbon powder.
Mixing straw carbon powder and dry powder of potato basic solid culture medium (every 4.6g of dry powder of potato basic solid culture medium contains 2g of glucose, 0.6g of potato and 2g of agar) for mixed culture:
no. 1, potato basic solid culture medium 4.6g
No. 2, 2.47g of dry powder of potato basic solid culture medium (agar mass is not counted) and 0.13g of straw carbon powder;
no. 3, 2.34g of dry powder of potato basic solid culture medium (agar mass is not counted) and 0.26g of straw carbon powder;
no. 4, 2.21g of dry powder of potato basic solid culture medium (agar mass is not counted) and 0.39g of straw carbon powder;
no. 5, 1.95g of dry powder of a potato basic solid culture medium (not counting the mass of agar) and 0.65g of straw carbon powder;
no. 6, 1.69g of dry powder of potato basic solid culture medium (agar mass is not counted) and 0.91g of straw carbon powder;
no. 7, 1.43g of dry powder (not counting the mass of agar) and 1.17g of straw carbon powder of potato basic solid culture medium;
no. 8, 1.04g of dry powder of a potato basic solid culture medium (agar mass is not counted) and 1.56g of straw carbon powder;
no. 9, 0.52g of dry powder (not counting the mass of agar) and 2.08g of straw carbon powder of potato basic solid culture medium;
no. 10, 2g agar (control group)
Except No. 1 and No. 10, agar 2g is added to each group, and No. 1-10 is dissolved in 100ml water and sterilized at 121 deg.C for 30 min. Adjusting the pH value to 6, culturing at 37 ℃ for 16 days after inoculation, wherein only 5% and 10% of groups can grow the phellinus igniarius (as shown in B in figure 1), and comparing A in figure 1 (a picture shot when culturing for 5 days), the agar contained in the solid culture medium has certain influence on the experimental result, the phellinus igniarius can also grow on the culture medium (No. 10) only containing the agar, and in order to avoid the influence, the subsequent culture is carried out by using the liquid culture medium, and meanwhile, because the growth effect of the phellinus igniarius in the culture medium added with the straw carbon powder is poorer than that of the culture medium only containing the potato, the corn straw carbon powder sediment obtained after the high-pressure kettle contains substances for inhibiting the growth of fungi.
Example 2
The corn stalks are dried after being cleaned, crushed by a centrifugal crusher and screened by a 100-mesh screen. Obtaining corn stalk fine powder, preparing a culture medium by using the corn stalk fine powder and water, and adding potato basic liquid culture medium dry powder (2 g of glucose and 0.6g of potatoes) according to different proportions.
Mixing dry powder of a potato basic liquid culture medium and corn straw powder according to different proportions:
no. 1, 2.6g of dry powder of potato basal liquid medium;
no. 2, 2.04g of dry powder of a potato basic liquid culture medium and 0.56g of straw powder;
no. 3, 1.56g of dry powder of a potato basic liquid culture medium and 0.94g of straw powder;
no. 4, 1.04g of dry powder of a potato basic liquid culture medium and 1.56g of straw powder;
no. 5, 0.52g of dry powder of a potato basic liquid culture medium and 2.08g of straw powder;
no. 6, straw powder 2.6g
No. 7, 100ml water (control group)
Dissolving the No. 1-6 substances in 100ml of water, and sterilizing at 121 ℃ for 30 min. Adjusting pH to 6, inoculating, culturing at 37 deg.C for 14 days, as shown in FIG. 2, the No. 5 culture medium has good growth vigor, i.e. 0.52g potato and 2.08g straw powder, and Phellinus linteus can also grow in the presence of only straw powder (No. 6 culture medium), and the No. 6 culture medium is the best culture medium for Phellinus linteus culture according to comprehensive consideration of material usage, yield, Phellinus linteus quality and price.
Example 3
The dry weight of the phellinus igniarius strains growing in the culture medium No. 1-6 in the figure 2 is measured after the phellinus igniarius strains are dried, and the phellinus igniarius yield in different culture media is calculated, wherein the yield results are shown in the table 1:
TABLE 1 yield results
| Medium number | Phellinus igniarius yield (g) |
| Number 1 | 0.308 |
| Number 2 | 0.438 |
| No. 3 | 0.619 |
| Number 4 | 0.790 |
| Number 5 | 0.956 |
| Number 6 | 0.886 |
The results of example 2 were further verified by weighing the dry weight of Phellinus linteus, and the experimental results were further determined by considering the material, yield, quality and price of Phellinus linteus comprehensively, with medium No. 6 being the best medium for Phellinus linteus culture.
And (4) conclusion: the invention discovers that the corn straws can also be used as a carbon source under appropriate conditions, the yield is higher in the same time compared with a commonly used potato culture medium, but the corn straws are easier to obtain and have economic benefits compared with potatoes, the culture medium is prepared by utilizing the corn straws, the problem of environmental pollution caused by difficulty in processing crop straws is solved, the resource recycling is realized, the prepared culture medium can be used as nutrient substances required by the growth of phellinus igniarius, the preparation method of the culture medium is simple, and a new path is provided for the culture of medicinal and edible fungi.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The culture medium for culturing phellinus igniarius is characterized by comprising corn straw powder and water as raw materials for preparing the culture medium.
2. The culture medium of claim 1, wherein the preparation method of the corn straw powder comprises the following steps: crushing and sieving the corn straws.
3. The culture medium according to claim 2, wherein the sieving condition is 100 mesh sieving.
4. The culture medium according to claim 1, wherein the mass ratio of the corn straw powder to the water in the culture medium is (0.1-2.6): 100.
5. a method for preparing the culture medium of any one of claims 1 to 4, comprising the steps of:
mixing the corn straw powder and water to obtain the culture medium for culturing the phellinus igniarius.
6. A culture medium for Phellinus linteus culture, wherein raw materials for preparing the culture medium comprise potato, sugar, corn stalk powder in the culture medium of claim 1 and water.
7. The culture medium according to claim 6, wherein the raw materials for preparing the culture medium comprise the following components in parts by weight: 0.1-2.6 parts of corn straw powder, 0.1-0.6 part of potato, 0-2 parts of sugar and 100 parts of water.
8. A method for preparing the medium of claim 6 or 7, comprising the steps of:
preparing a potato basal culture medium by taking potatoes and sugar as raw materials; and mixing the potato basal culture medium, the corn straw powder and water to obtain the culture medium for culturing the phellinus igniarius.
9. A method for liquid culture of Phellinus linteus, comprising inoculating Phellinus linteus into the culture medium according to any one of claims 1 to 4 or the culture medium according to claim 6 or 7, and culturing to obtain Phellinus linteus; the temperature of the culture is 25-37 ℃, and the pH value of the culture is 4-8.
10. Application of corn stalk in culturing Phellinus Linteus is provided.
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