CN103396192A - Manufacturing method of liquid fermentation medium of trace element-rich medicinal fungus - Google Patents
Manufacturing method of liquid fermentation medium of trace element-rich medicinal fungus Download PDFInfo
- Publication number
- CN103396192A CN103396192A CN2013103401911A CN201310340191A CN103396192A CN 103396192 A CN103396192 A CN 103396192A CN 2013103401911 A CN2013103401911 A CN 2013103401911A CN 201310340191 A CN201310340191 A CN 201310340191A CN 103396192 A CN103396192 A CN 103396192A
- Authority
- CN
- China
- Prior art keywords
- medicinal fungus
- trace element
- liquid
- fermentation medium
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a manufacturing method of a liquid fermentation medium of a trace element-rich medicinal fungus, a liquid mycelium fermentation medium formula of the trace element-rich medicinal fungus is designed and prepared as follows: a yeast fermentation liquid concentrate is used as a main nitrogen source, a mixture of rice, wheat and corn straw powder is used as a main carbon source, and at the same time four trace elements comprising chromium, magnesium, zinc and selenium are added. A yeast fermentation liquid is effectively recycled, the nitrogen source cost is greatly reduced, the trace elements in the mediumare transformed into organic trace element forms after fermentation and transformation, and can be used as trace element supplements.
Description
Technical field
The present invention relates to a kind of culture medium prescription making method of medicinal fungus liquid fermenting, particularly relate to the liquid fermentation medium making method of the little medicinal fungus of a kind of richness, belong to the bio-pharmaceuticals production technical field.
Background technology
Medicinal fungus broad sense refer to that all can be used for the fungus of pharmacy, narrow sense refers to the macro fungi (gill fungus bacterium) for medicine.Wild have Cordyceps sinensis, cicada fungus, stone-like omphalia, a CNNS's skin umbrella etc.; At present, tame have glossy ganoderma, Poria cocos, umbellate pore furgus, rhizoma Gastrodiae, Phellinus, auricularia auriculajudae, a rainbow conk etc.These medicinal fungus have the applicating history of more than one thousand years in China, they are though effect is different, and maximum advantage, be also that their common ground has no side effect exactly.The functions such as modern medicine research shows, they are not only traditional beneficial gas, keep fit, get rid of illness, stimulate the menstrual flow, lengthen one's life, also tool strengthens body immunity, antitumor anticancer effect.Many edible mushroomss are with pharmaceutical use.
China utilizes fungi as the existing at least history in 2500 of medicine therapy.China the earliest medicine book Shennong's Herbal and the successive dynasties herbal medicine in record: the medicinal fungis such as glossy ganoderma, Poria cocos, umbellate pore furgus, Cordyceps sinensis.China's Resources of Medicinal Fungi is abundant, and identified just have a kind more than 200, to the medicinal fungi systematic research since the sixties in 20th century.Early stage work focuses on artificial culture research, as artificial cultures such as Poria cocos, hedgehog hydnum, glossy ganodermas, studies.Since the seventies, the exploitation of medicinal fungi have been used as medicine and have been developed into Industrial Submerged Fermentation from early stage use sporophore compatibility, physics, chemical process extracting effective components, the reconstruction structure composition, route of administration except oral, has also developed injection.Simultaneously, also done a large amount of work at aspects such as pharmacology, medicine, clinical experiment, pharmaceutical technologies.
The exploitation of medicinal fungus is generally to extract from the sporophore of natural or artificial culture at present, yet fruiting body yield is limited, and along with the development of liquid fermentation technology, the advantage of cultivating medicinal fungi by solution fermentation progressively embodies.Adopt liquid culture method to produce medicinal fungus mycelium and fermented liquid, not only the cycle is short, condition is simple, output is high, but also can improve the output of its biologically active substance by the optimization production condition, facilitates the extraction of purpose component.Therefore, be devoted to the mycelial artificial culture of medicinal fungus both at home and abroad always,, to shorten incubation time, save production cost.Occurred more medicinal fungus mycelium product on domestic market, mostly be liquid submerged fermentation and cultivate gained.
Selenium is a kind of essential trace element of humans and animals, is the important component part of plurality of enzymes, and selenium is the sweet skin peroxidase of paddy moon bright important component, and by redox, the protection microbial film is avoided infringement, keeps the normal function of cell.Selenium can also play strengthening immunity, protection cardiovascular, promote growth, antitumor, detoxifcation, regulate the function such as metabolism.The endemy that causes at the scarce selenium of China has Keshan disease and Kaschin-Beck disease.Yeast rich in selenium, for supplementing the some diseases successful that causes because lacking selenium, has very abundant nutritive ingredient in addition in yeast, can bring into play synergy.
The chromium element plays an important role in all Regular Insulin adjusting activities as a kind of micronutrient element of necessity, and it can help Regular Insulin to promote that glucose enters intracellular efficiency, is important blood sugar regulator.Aspect blood glucose regulation, particularly the diabetic subject there is important effect.It helps to grow, and the cholesterol concentration in blood is also had control action kou, may cause heart disease during shortage.
Magnesium elements, as the activator of enzyme, participates in the enzymatic reaction more than 300 kinds.Synthetic, the nucleic acid metabolism of glycolysis-, Fatty Acid Oxidation, protein etc. needs magnesium ion to participate in.It is only second to calcium, phosphorus in bone, be the necessary element of osteocyte structure and function, and to promoting bone growing and osteanagenesis, intensity and the density of keeping bone and tooth have vital role.
Magnesium, calcium, potassium ion are worked in coordination with and are kept nervimuscular excitability simultaneously.In blood, magnesium is too low or calcium is too low, and excitability all increases; Otherwise sedative effect is arranged.The most important thing is that magnesium can improve the vigor of sperm, strengthen male fecundity, magnesium is the male sex's " health-care extract ".
The relation of zinc element and brain development and intelligence, promote lymphopoiesis and mobility, epithelium and mucosal tissue are normal to keeping, defense against bacterial, Virus entry, elimination ulcer, promote wound healing, reduce the dermatosis such as acne and proofread and correct the sense of taste malfunctioning, improve appetite, strengthen the dark situation adaptive faculty etc.
Produce in medicinal fungus mycelium and fermentation liquor technique at liquid culture method, the formula of substratum is very crucial.Present rich little medicinal fungus liquid fermenting formula is nitrogenous source mainly with extractum carnis, peptone or common yeast powder greatly, take sucrose, glucose or rice as main carbon source, the component of formula lacks specific aim, cause the active principle content such as exocellular polysaccharide in mycelium, fermented liquid and medicinal rhzomorph, medicinal fungus acid not high, production cost rises.In fact, in the liquid bacteria mycelium fermentation process of the little medicinal fungus of richness, the nitrogen carbon sources such as extractum carnis, peptone, sucrose, glucose or rice are not only on the high side, and not suitable nitrogen carbon source, be difficult for therefore must designing and producing the liquid bacteria mycelium fermentation culture medium prescription of the high rich little medicinal fungus of a kind of inexpensive effect for mycelium absorbs.
Summary of the invention
Purpose of the present invention, design exactly a kind of with take the yeast fermentation broth enriched material as major nitrogen source, take rice, wheat, maize straw powder mixture as main carbon source, add simultaneously chromium, magnesium, zinc, four kinds of trace elements of selenium, the liquid bacteria mycelium fermentation culture medium prescription of the rich little medicinal fungus of the liquid bacteria mycelium fermentation culture medium prescription of the rich little medicinal fungus that is made.
The liquid fermentation medium making method of now introducing the little medicinal fungus of a kind of richness is as follows:
1) yeast fermentation broth enriched material: the yeast fermentation broth enriched material of selecting market supply;
2) preparation of carbon source component raw material: with rice, wheat, maize straw powder mixture according to mass ratio: mix at 1: 1: 2, be crushed to the 50-100 order, standby;
3) preparation of trace element: with chromium, magnesium, zinc, selenium element according to mass ratio 10: 10: 3: 3 ratio is mixed.
4) the liquid fermentation medium formula of rich little medicinal fungus is (each component is mass percent): yeast fermentation broth enriched material 3.0-4.5%, carbon source component raw material 3.0-4.0%, KH
2PO
40.1-0.3%, vitamins B
10.01-0.03%, last adding distil water adds to 100%, transfers pH6.0-7.0,121 ℃ of sterilizing 15-20min, cooling after namely accessible medicinal fungus seed liquor ferment.
Major advantage of the present invention is:
Yeast fermentation broth enriched material in substratum is playing the effect while of supplementing nitrogenous source, or the composition that rises in fermenting culture also can promote the growth of medicinal fungus.
Carbon source material in the present invention is rice, wheat, maize straw powder mixture.Because VITAMIN and other micro-factor abundant in coarse food grain have promoted well carrying out of metabolism, with the composite rear use of these coarse food grains, providing of main carbon source starch both had been provided, the lot of trace factor is complemented one another, fully met the needs of fermentation, and cost is far below conventional carbon sources such as dextrose plus saccharoses.
Embodiment
1) yeast fermentation broth enriched material: the yeast fermentation broth enriched material of selecting market supply;
2) preparation of carbon source component raw material: with rice, wheat, maize straw powder mixture according to mass ratio: mix at 1: 1: 2, be crushed to the 50-100 order, standby;
3) preparation of trace element: with chromium, magnesium, zinc, selenium element according to mass ratio 10: 10: 3: 3 ratio is mixed.
4) the liquid fermentation medium formula of rich little medicinal fungus is (each component is mass percent): yeast fermentation broth enriched material 3.0-4.5%, carbon source component raw material 3.0-4.0%, KH
2PO
40.1-0.3%, vitamins B
10.01-0.03%, last adding distil water adds to 100%, transfers pH6.0-7.0,121 ℃ of sterilizing 15-20min, cooling after namely accessible medicinal fungus seed liquor ferment.
Claims (1)
1. the liquid fermentation medium making method of the little medicinal fungus of richness is characterized in that:
1) yeast fermentation broth enriched material: the yeast fermentation broth enriched material of selecting market supply;
2) preparation of carbon source component raw material: with rice, wheat, maize straw powder mixture according to mass ratio: mix at 1: 1: 2, be crushed to the 50-100 order, standby;
3) preparation of trace element: with chromium, magnesium, zinc, selenium element according to mass ratio 10: 10: 3: 3 ratio is mixed.
4) the liquid fermentation medium formula of rich little medicinal fungus is (each component is mass percent): yeast fermentation broth enriched material 3.0-4.5%, carbon source component raw material 3.0-4.0%, KH
2PO
40.1-0.3%, vitamins B
10.01-0.03%, last adding distil water adds to 100%, transfers pH6.0-7.0,121 ℃ of sterilizing 15-20min, cooling after namely accessible medicinal fungus seed liquor ferment.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2013103401911A CN103396192A (en) | 2013-08-03 | 2013-08-03 | Manufacturing method of liquid fermentation medium of trace element-rich medicinal fungus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2013103401911A CN103396192A (en) | 2013-08-03 | 2013-08-03 | Manufacturing method of liquid fermentation medium of trace element-rich medicinal fungus |
Publications (1)
Publication Number | Publication Date |
---|---|
CN103396192A true CN103396192A (en) | 2013-11-20 |
Family
ID=49559982
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2013103401911A Pending CN103396192A (en) | 2013-08-03 | 2013-08-03 | Manufacturing method of liquid fermentation medium of trace element-rich medicinal fungus |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103396192A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114375767A (en) * | 2022-01-11 | 2022-04-22 | 吉林大学 | Culture medium for culturing phellinus igniarius, and method and application for culturing phellinus igniarius |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1297995A (en) * | 2001-01-12 | 2001-06-06 | 杨忠才 | Production process of chromium-rich cordyceps mycelium |
CN1443568A (en) * | 2002-03-08 | 2003-09-24 | 胡征 | Preparation method of ganoderma biological activity multivitamin intesifying agent |
CN101113415A (en) * | 2007-07-08 | 2008-01-30 | 曾建军 | Method for solid culture of Cordyceps sinensis edible mushroom |
CN101904497A (en) * | 2010-07-16 | 2010-12-08 | 湖北工业大学 | Method for improving vitamin D content in lucid ganoderma calcium fortifier by ultraviolet irradiation |
CN101904498A (en) * | 2010-07-16 | 2010-12-08 | 湖北工业大学 | Method for improving vitamin D content in cordyceps sinensis calcium strengthening agent by ultraviolet irradiation |
CN101971763A (en) * | 2010-03-22 | 2011-02-16 | 陈顺志 | Industrial energy-saving and low-carbon method for cultivating lucid ganoderma |
KR20120006124A (en) * | 2010-07-12 | 2012-01-18 | 정경자 | Method of manufacture for organic fertilizer using feather |
-
2013
- 2013-08-03 CN CN2013103401911A patent/CN103396192A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1297995A (en) * | 2001-01-12 | 2001-06-06 | 杨忠才 | Production process of chromium-rich cordyceps mycelium |
CN1443568A (en) * | 2002-03-08 | 2003-09-24 | 胡征 | Preparation method of ganoderma biological activity multivitamin intesifying agent |
CN101113415A (en) * | 2007-07-08 | 2008-01-30 | 曾建军 | Method for solid culture of Cordyceps sinensis edible mushroom |
CN101971763A (en) * | 2010-03-22 | 2011-02-16 | 陈顺志 | Industrial energy-saving and low-carbon method for cultivating lucid ganoderma |
KR20120006124A (en) * | 2010-07-12 | 2012-01-18 | 정경자 | Method of manufacture for organic fertilizer using feather |
CN101904497A (en) * | 2010-07-16 | 2010-12-08 | 湖北工业大学 | Method for improving vitamin D content in lucid ganoderma calcium fortifier by ultraviolet irradiation |
CN101904498A (en) * | 2010-07-16 | 2010-12-08 | 湖北工业大学 | Method for improving vitamin D content in cordyceps sinensis calcium strengthening agent by ultraviolet irradiation |
Non-Patent Citations (1)
Title |
---|
洪培 等: "2 种真菌培养液对食用菌菌丝体生长的影响", 《中国食用菌》, vol. 32, no. 2, 30 April 2013 (2013-04-30), pages 33 - 35 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114375767A (en) * | 2022-01-11 | 2022-04-22 | 吉林大学 | Culture medium for culturing phellinus igniarius, and method and application for culturing phellinus igniarius |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102060578B (en) | Method for producing microecological foliar fertilizer by utilizing probiotics and microalgae | |
CN101129174B (en) | Process for preparing organic selenium pine pollen | |
CN102210388B (en) | Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction | |
CN105559089A (en) | Liver-protecting and toxin-expelling fruit and vegetable enzyme and preparation method thereof | |
CN103609897B (en) | Preparation method and application of Chinese herbal immunopotentiator for Salmo trutta marmoratus | |
CN102771314A (en) | Method for culturing selenium-enriched cordyceps militaris | |
CN110403073A (en) | A kind of soybean stalk fermented feed and preparation method thereof | |
CN106148200B (en) | culture medium of selenium-rich low-lead and arsenic cordyceps sinensis mycelia and cultivation method of culture medium | |
CN103864523A (en) | Mushroom residue culture base material and fabrication method thereof | |
CN103299824B (en) | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn | |
CN102577839B (en) | Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium | |
CN103704017A (en) | High-nutrition formula of cordyceps culture medium | |
CN107594084A (en) | A kind of non-resistant ecological feed formula of growing and fattening pigs | |
CN104522750A (en) | Alga healthcare food | |
CN101613652A (en) | Tuckahoe health care white spirit and preparation method thereof | |
CN104945129A (en) | Mushroom culture medium | |
CN108383652A (en) | Agricultural wastes cultivate rich selenium germanium green organic element nutrition fertilizer | |
CN1826866A (en) | Method for planting common fig or fiveleaf gynostemma herb with enriched microelement and uses thereof | |
CN105533632A (en) | Method for preparing low-sodium nutritional salt substitute with liquid state fermentation technology | |
CN109006182A (en) | A kind of culture base-material and preparation method thereof with Lenlinus edodes slag for cultivating oyster mushroom | |
CN109650959A (en) | A kind of mushroom planted medium and preparation method thereof | |
CN103396192A (en) | Manufacturing method of liquid fermentation medium of trace element-rich medicinal fungus | |
CN109180264A (en) | The micro- fertilizer of the decomposed bacterium of fermented and cultured rich selenium germanium | |
CN106343196A (en) | Feeding yeast additive which makes the pork more nutrient, fresh, tender and delicious and preparing method thereof | |
CN102894428A (en) | Dense schizophyllum commune red date milk |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C05 | Deemed withdrawal (patent law before 1993) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131120 |