Summary of the invention:
The object of the present invention is to provide a kind of preparation method of Ganoderma biological activity multidimensional hardening agent, it is the biological activity hardening agent that through liquid fermentation chemical elements such as ferrum, zinc, calcium, selenium, chromium is changed into biomacromolecule chelating attitude by Cordyceps fungus, product is rich in multivitamin, thalline immune protein and thalline polysaccharide, has immunoloregulation function.
In order to realize above-mentioned task, the present invention adopts following solution:
The medicinal fungus Ganoderma is a rare Chinese medicine, its health care and Drug therapy effect are early developed by the traditional Chinese medical science, and the Ganoderma that studies confirm that of modern science truly has pharmacologically active very widely, and particularly Ganderma lucidum has immunoregulation effect, regulating immunological function suppresses anaphylaxis; Regulate vegetative nerve function, correct high partially phenomenon (antitussive, eliminate the phlegm, relieving asthma).Therefore, Ganoderma is widely used clinically, can treat chronic bronchitis, asthma, allergic rhinitis, progressive muscular dystrophy.Taking behind the Ganoderma medicine obvious characteristics is that appetite increases, and immunity strengthens.Since multiple factors such as heredity, growth, physiology, the frequent disease that hypoimmunity, hormonal system hypofunction and so on take place in child, women, old man crowd.It is the carrier of biological activity hardening agent that the present invention selects Ganderma lucidum for use, makes product possess the critical function of regulating immunity.
The solution of existing hardening agent defective is to adopt the method for microbial transformation, chemical substance is absorbed the biological activity hardening agent that is converted into biomacromolecule chelating attitude, make it have biological homology, balance, and Ganderma lucidum is rich in multivitamin such as vitamin D, the promotion Absorption is arranged, help improving its bioavailability.
Simultaneously,, has enhancing immunity, the function of adjusting digestive system by studies have shown that the present invention because function of immune system missionary society influence digestive system to the digesting and assimilating of nutrient.
Ganderma lucidum has enrichment, transforms the ability of mineral element, chemical elements such as ferrum, zinc, calcium, selenium, chromium can be converted into the biological activity hardening agent.
The present invention confirms that by experiment Ganderma lucidum transforms the absorption of mineral:
A, be formulated as follows culture medium:
Peptone 1.5g
Glucose 4.5g
KH
2PO
4 0.1g
MgSO
4 0.02g
VB
1 0.001g
With FeSO
4Add ferrum: 0.01g
With ZnSO
4Add zinc: 0.008g
With CaCl
2Add calcium: 0.4g
With NaSeO
3Add selenium: 0.00002g
With CrCl
3Add chromium: 0.00002g
Be settled to 100ml after adding water-solubleization;
With every bottle of 100ml packing 300ml triangular flask, below 30 minutes postcooling to 30 of 120 ℃ of sterilizations ℃;
B, to the liquid Ganderma lucidum strain of above-mentioned inoculation of medium 2% volume, shake-flask culture is 80 hours under 26-30 ℃ of condition, takes out fermentation liquid, with 10000g high speed centrifugation 30 minutes, collects supernatant and bacterial sediment respectively;
C, bacterial sediment add 1ml concentrated nitric acid and 10ml water, heat nitratedly, are settled to 100ml, with the content of aas determination ferrum, zinc, calcium, selenium, chromium; With supernatant ultrafiltration (ultrafilter membrane is held back 2000 above macromole), use the calcium content of measuring filtrate with quadrat method;
D, result:
The percentage ratio that the content of above-mentioned five kinds of mineral elements accounts for addition in the Ganoderma thalline is respectively:
Ferrum: 99.8%; Zinc: 99.6%; Selenium: 99.7%; Chromium: 99.5%; Calcium: 83.5%.
The content of calcium constituent is 3.2% of addition in the ultrafiltrate.
The above results explanation: for ferrum, zinc, selenium, the chromium plasma of few additive, the Ganoderma thalline almost can all absorb to cell, and according to knowledge biology, they are the important component that can constitute multiple auxilliary acid and hormone with vitamin; For the higher calcium ion of addition, having an appointment 80% absorbs to cell, and all the other main and born of the same parents are biological macromolecular substances chelatings outward, and having only a few percent is free calcium ions.Gain knowledge according to microbiology and cell biological, the Ca-pump is arranged on the eukaryotic cell membrane, this transmembrane protein mainly is present on cytoplasma membrane and the endoplasmic reticulum, it pumps into calcium ion in the endoplasmic reticulum, calbindin more than 4 kinds is arranged in the endoplasmic reticulum, each calbindin molecule can with 30 left and right sides calcium ion chelatings.These calbindin concentration in the endoplasmic reticulum can reach 30-100mg/ml, can in conjunction with, store a large amount of calcium ions.Contain a large amount of polysaccharide, organic acid, protein and other in the extracellular fermentation liquid, they equally can with the calcium ion chelating.
According to above-mentioned design and experimental result, realize that the object of the invention adopts following technical scheme:
The microorganism of using:
Produce the various strains of the Basidiomycetes Ganoderma (Ganoderma Karst.) of medicinal ingredient, they neither binding neither be exclusive.
Concrete bacterial strain can be:
In China Committee for Culture Collection of Microorganisms's preservation, preserving number is Ganoderma (Ganoderma lucidum) bacterial strain of CGMCC No.5.535.The related properties of above-mentioned strain can be consulted Chinese common micro-organisms DSMZ (CGMCC) strain catalogue, and the present invention repeats no more.Anyone can directly buy this strain to this preservation center.
Biological activity multidimensional hardening agent is to be prepared from through liquid fermentation, concentrate drying by Ganderma lucidum, the steps include:
A, culture medium system are joined: prepare fluid medium with carbon source, nitrogenous source, mineral element, add following ingredients (weight portion) in every 100ml culture medium:
Ferrum 0.001~0.1
Zinc 0.001~0.1
Calcium 0.1~1.0
Selenium 0.000001~0.0001
Chromium 0.000001~0.0001;
B, with prepared culture medium sterilization, below 30 minutes postcooling to 30 of 120 ℃ of sterilizations ℃;
C, in culture medium, add 2~10% Ganderma lucidum liquid strain;
D, above-mentioned culture fluid was cultivated 50~100 hours 20 ℃~30 ℃ following air agitations, obtained liquid form product;
E, liquid fermentation product are broken and crushed into powder products through concentrated, dry.
Carbon source in the described culture medium comprises 2~8% saccharide such as glucose, sucrose, molasses, or starch based such as cereal starch, Semen Maydis powder, sweet potato powder a kind of or form below six kinds;
Nitrogenous source comprises a kind of or composition below four kinds of 1~4% yeast powder, analysis for soybean powder, peanut cake powder, Semen Maydis pulp;
Carbon, nitrogen ratio are 10: 1~40: 1;
Mineral element is that materials such as potassium, magnesium, phosphorus, sodium, sulfur are an amount of;
Described biological activity multidimensional hardening agent can be used for the preparation treatment and prevent immunization programs for children power low with beverage, oral liquid, chewable tablet, confection, capsule or other dosage form, child's calcium deficiency, iron deficiency anemia; Human female is weak, physiologic anemia; The medicine of various diseases such as old man's immunity recession, osteoporosis or the application in the health food.
Contain following component (weight portion) in every 100g hardening agent product:
Bacterium protein 25.0~45.0
Total sugar (comprising the thalline polysaccharide) 2.0~10.0
Carbohydrate 20.0~40.0
Total fat 2.0~10.0
Mineral 15.0~25.0
Moisture 4.0~10.0
Ferrum 0.1~1.0
Zinc 0.1~1.0
Calcium 2.0~20.0
Selenium 0.0005~0.005
Chromium 0.0005~0.005
Vitamin A 0.001~0.04
Vitamin B1 0.02~0.1
Vitamin B2 0.02~0.1
Vitamin B5 0.2~1.5
Vitamin B6 0.01~0.2
Vitamin B12 0.0005~0.001
Vitamin C 1.0~5.0
Vitamin D 0.0005~0.005
Vitamin E 0.00001~0.0001
Folic acid 0.0001~0.005
The present invention compared with prior art has the following advantages and effect:
Production technology is simple, adopts the method for microbial transformation, and the chemical element that human body is difficult for absorbing absorbs and is converted into the biological activity hardening agent that biomacromolecule is integrated attitude, makes it have biological homology, and balance helps improving the bioavailability of product.Utilize the multivitamin characteristic of Ganderma lucidum energy biosynthesis simultaneously, make product have additional multivitamin concurrently, promote the function of alimentation.Add the distinctive immunomodulating pharmacology of Ganoderma activity, make product possess multi-efficiency, can be widely used in the preparation treatment and prevent immunization programs for children power low, child's calcium deficiency, iron deficiency anemia; Human female is weak, physiologic anemia; The medicine of the various diseases such as osteoporosis that old people's immunity recession, endocrine disturbance cause or the application in the health food.