CN114365750B - Roundworm killing reagent and application thereof - Google Patents
Roundworm killing reagent and application thereof Download PDFInfo
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- CN114365750B CN114365750B CN202111610397.2A CN202111610397A CN114365750B CN 114365750 B CN114365750 B CN 114365750B CN 202111610397 A CN202111610397 A CN 202111610397A CN 114365750 B CN114365750 B CN 114365750B
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- 241000244203 Caenorhabditis elegans Species 0.000 title claims abstract description 32
- 230000002147 killing effect Effects 0.000 title claims abstract description 22
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 7
- 241001233983 Orychophragmus violaceus Species 0.000 claims abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 27
- 235000013601 eggs Nutrition 0.000 claims abstract description 24
- 241000244206 Nematoda Species 0.000 claims abstract description 14
- 239000004480 active ingredient Substances 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 41
- DQYBDCGIPTYXML-UHFFFAOYSA-N ethoxyethane;hydrate Chemical compound O.CCOCC DQYBDCGIPTYXML-UHFFFAOYSA-N 0.000 claims description 20
- 239000012259 ether extract Substances 0.000 claims description 17
- 239000000284 extract Substances 0.000 claims description 17
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 7
- 229930003268 Vitamin C Natural products 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- 235000019154 vitamin C Nutrition 0.000 claims description 7
- 239000011718 vitamin C Substances 0.000 claims description 7
- QTYWBJZOZDYCGB-UHFFFAOYSA-L potassium;sodium;2-carboxybenzoate;hydroxide Chemical compound [OH-].[Na+].[K+].OC(=O)C1=CC=CC=C1C([O-])=O QTYWBJZOZDYCGB-UHFFFAOYSA-L 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000005360 mashing Methods 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 230000009471 action Effects 0.000 abstract description 3
- 238000000746 purification Methods 0.000 abstract description 2
- 230000000903 blocking effect Effects 0.000 abstract 1
- 238000009264 composting Methods 0.000 description 13
- 239000010802 sludge Substances 0.000 description 12
- 244000052769 pathogen Species 0.000 description 11
- 241000238631 Hexapoda Species 0.000 description 7
- 238000001035 drying Methods 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 6
- 230000002779 inactivation Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 3
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 235000011941 Tilia x europaea Nutrition 0.000 description 3
- 239000002361 compost Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000004571 lime Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000244186 Ascaris Species 0.000 description 1
- 241000244185 Ascaris lumbricoides Species 0.000 description 1
- 241000244188 Ascaris suum Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003736 gastrointestinal content Anatomy 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000007602 hot air drying Methods 0.000 description 1
- 238000007603 infrared drying Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M99/00—Subject matter not provided for in other groups of this subclass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a roundworm killing reagent and application thereof, belonging to the field of environmental purification. Specifically, the invention provides application of orychophragmus violaceus as an active ingredient in preparation of a reagent for killing roundworm and/or roundworm eggs. In addition, the invention also provides a method for killing roundworms or roundworm eggs in the environment by using the orychophragmus violaceus as an active ingredient. The orychophragmus violaceus provided by the invention is used as an active ingredient to kill roundworms or roundworm eggs, has the characteristics of good effect, low action concentration and short response time, and provides a new idea for killing roundworms and blocking propagation in the environment.
Description
Technical Field
The invention belongs to the field of environmental purification, and particularly relates to a roundworm killing reagent and application thereof.
Background
Along with the development of economy and the progress of urbanization work in China, the sludge produced by urban sewage is greatly increased. According to statistics, the amount of dry sludge generated in China every year is about 800 ten thousand tons. On the other hand, the soil resources available for planting are very scarce. Therefore, land utilization of sludge is an economical and effective disposal means for solving the problem of sludge export.
At present, common pathogen inactivation methods mainly comprise high-temperature drying, alkaline stabilization, composting and the like. Among them, high-temperature drying has been studied at home and abroad, and the drying methods include hot air drying, gas infrared drying, electric heating drying, and the like. Corresponding granulation equipment is generally required to be matched, and the time required for pathogen inactivation is related to the drying temperature, but the method has great defects, firstly, the requirement on the temperature resistance of equipment materials is high, and the energy consumption of heating and stirring is great; meanwhile, volatile organic substances in the sludge escape along with water vapor in the drying process, so that secondary pollution is easily caused. The alkaline stabilization mainly utilizes chemical reaction to raise temperature, and lime, alumina or ammonia water and the like are added to react with organic acid in sludge to achieve the effect of raising temperature to remove pathogens. The results of the practical operation show that the alkaline method can quickly inactivate the pathogens in the sludge stably (about 2 hours), but the method has the defects: the pH value of the sludge after the alkaline process stabilization is too high, the property is unstable, subsequent treatment is necessary, otherwise, land utilization cannot be carried out; the physicochemical property of the sludge stabilized by lime is changed, when the lime is applied to the soil for utilization, the soil can be hardened, and the stacking area and the transportation cost of the sludge are increased on the contrary because the quantity is not reduced; the cost of introducing ammonia gas is high, the residual ammonia gas also needs to be treated, and the leakage of ammonia gas is easy to cause accidents. Composting, which is a technology applied to organic garbage and makes a great deal of research, including static composting and dynamic composting, namely a spontaneous composting method, a cylindrical divided-grid closed composting method, a roller composting method, a vertical multilayer reaction composting method, a strip-shaped static ventilation composting method and the like. The treatment of sludge by composting technology has also been studied at home and abroad. These composting processes have a common drawback due to the abundant organic matter contained in the sludge: a) The compost is not uniform, and the pathogen on the surface of the compost can be proliferated, so that the inactivation of the pathogen cannot be well guaranteed; b) The survival ability of the dominant microbial population is weaker, the influence of the environmental temperature is larger, the treatment period in summer is not less than 20 days, and the treatment period in winter is not less than 30 days; c) The inhibition effect on odor in the composting process is poor, and malodorous gas is easily emitted; d) The static compost is slowly heated and cannot well meet the requirement of pathogen inactivation; the dynamic composting equipment is complex, the oxygen supply condition has certain problems, such as the adoption of a bottom perforated pipe for oxygen supply, uneven mass transfer, easy blockage and possible generation of percolate to cause secondary pollution. The above method is also not suitable for the disinfection of pathogens in septic tanks, particularly in the first compartment.
Therefore, there is a need to develop a pathogen killing technology which is efficient, convenient to use, low in cost and applicable to a wide range of scenes.
Disclosure of Invention
Aims to solve the problems that the prior art has residual pathogens in domestic sewage, particularly residual roundworm eggs, and the prior art is lack of a method which can kill pathogens, such as roundworm eggs, efficiently and conveniently. The invention provides the following technical scheme:
in one aspect, the invention provides the application of orychophragmus violaceus as the single active ingredient in preparing the reagent for killing roundworms and/or roundworm eggs.
In the invention, orychophragmus violaceus, also called Orychophragmus violaceus (L.) O.E. Schulz, is a cruciferae, orychophragmus violaceus, a year or two-year herbaceous plant with the height of 50 cm and no hair; the stem is upright, the basal leaf and the lower cauline leaf have big-end pinnate full cleft, the top lobe is approximately round or short oval, the side lobe is oval or triangular oval, and the leaf stalk has the functions of thinning out fine and soft hair; the flower is purple, light red or faded to white, the calyx is cylindrical, purple, the petals are wide and inverted egg-shaped, dense and thin vein lines and long-horn fruit line shapes, the seeds are oval to long round, black brown, blossom is in 4-5 months, and fruit bearing is in 5-6 months.
According to a specific embodiment of the present invention, orychophragmus violaceus comprises the whole herb, the effective part and/or the extract thereof.
According to a specific embodiment of the invention, the orychophragmus violaceus is an organic solvent extract of the whole grass and/or the effective part of orychophragmus violaceus.
According to a specific embodiment of the present invention, the organic solvent includes one or more of ethanol, dichloromethane, diethyl ether and petroleum ether.
According to a specific embodiment of the present invention, orychophragmus violaceus is a water-ether extract of the whole herb thereof.
According to a specific embodiment of the invention, the water-ether extract of the whole plant of orychophragmus violaceus is prepared according to the following method:
taking 500-1000g of February herb, mashing, and adding 2000-3000 mL of potassium hydrogen phthalate-sodium hydroxide buffer solution and 20-50mL of vitamin C aqueous solution; standing for 8-16h under a sealed condition; standing, distilling under reflux for 6-8h, cooling to room temperature, and filtering to obtain filtrate; extracting the filtrate with diethyl ether for 3-4 times, mixing diethyl ether extractive solutions, and removing diethyl ether in the diethyl ether extractive solution in water bath to obtain water-diethyl ether extract of February.
In the invention, in the process of preparing the orychophragmus violaceus extract, the aqueous solution of potassium hydrogen phthalate-sodium hydroxide and vitamin C is added, so that the leaching degree of the active ingredients in orychophragmus violaceus can be improved to the maximum extent, and meanwhile, the stability of the active ingredients can be improved by adding the vitamin C, and certain ingredients in the orychophragmus violaceus extract are prevented from being oxidized.
According to a specific embodiment of the present invention, the pH of the potassium hydrogen phthalate-sodium hydroxide buffer is 5.0.
According to a particular embodiment of the invention, the concentration of the aqueous solution of vitamin C is between 0.2% and 0.5%.
In another aspect, the invention also provides an extract of orychophragmus violaceus, which is prepared by the method.
In addition, the present invention also provides a method of killing roundworm eggs and/or roundworm in an environment, comprising:
applying the orychophragmus violaceus extract in the invention to an environment needing to kill roundworm eggs and/or roundworms; wherein:
when the environment needing to be killed is a dry environment, preparing the orychophragmus violaceus extract into an aqueous solution with the concentration of 0.1-5%, and then spraying the aqueous solution into the environment to be killed;
when the environment needing killing is a water body environment, the orychophragmus violaceus extract is directly added into the water body, and the adding amount is 0.1-5% of the concentration of the orychophragmus violaceus after dissolution.
In summary, the present invention provides a new method for destroying roundworm and roundworm eggs. In the invention, the water-ether extract of the orychophragmus violaceus is used as an effective component for killing roundworms, and has the following beneficial effects:
(1) The orychophragmus violaceus is a common herbaceous plant, and the raw materials are cheap and easy to obtain, so that the prevention and treatment cost is low;
(2) The extraction method provided by the invention is relatively simple and easy to realize;
(3) Compared with the method for removing roundworms in the prior art, the method provided by the invention adopts the effective components extracted from natural substances, so that the method is more environment-friendly;
(4) The orychophragmus violaceus extract provided by the invention is good in killing effect and low in effective concentration.
Detailed Description
The technical aspects of the present invention will now be described in detail below in order to clearly understand the technical features, objects, and advantages of the present invention, but the present invention is not limited thereto.
Examples
1.1 preparation of February Water-Ether extract
Taking 500g of February herb, mashing, and adding 2000-3000 mL of potassium hydrogen phthalate-sodium hydroxide buffer solution, wherein the pH value is 5.0 (Annagi chemical), and 20-50mL of vitamin C (Annagi) aqueous solution, and the mass fraction is 0.2%; standing for 8 hours under a sealed condition; standing, distilling for 8h under reflux, cooling to room temperature, and filtering to obtain filtrate; extracting the filtrate with diethyl ether for 4 times, mixing the diethyl ether extractive solutions, and removing diethyl ether from the diethyl ether extractive solutions in water bath to obtain water-diethyl ether extract of February. The resulting February water-ether extract was diluted with sterile water to various concentrations.
1.2 contact treatment of roundworms
Respectively placing live insect bodies and live insect eggs in February extract solutions with different concentrations at 36 ℃, recording action time, taking out the insect bodies for checking death conditions at different times for 2min, 3min, 4min, 5min, 6min, 7min, 8min, 9min and 10min, placing the insect eggs in sterile fresh survival liquid, incubating at 37 ℃ for 3-7 days, and checking survival conditions at regular time (if no insect bodies appear after 7 days, the insect eggs are killed). Meanwhile, sterile water treatment was used as a control. Wherein the insect body and egg are derived from pig roundworm. The composition of the living liquid of the polypide is as follows: beef extract 0.1%, peptone 0.2%, naCl 0.1%, glycerin 1%, and pig intestine and stomach content filtrate 5%, mixing, sterilizing, and placing in a vacuum flask at 37 deg.C.
1.3 results of the experiment
1.3.1 February Water-Ether extract against live roundworm
The elimination effect of 15 roundworms as experimental subjects was observed by using the orychophragmus violaceus extract with different concentrations at different treatment times, and the experimental results are shown in the following table 1:
TABLE 1
As can be seen from the data in table 1, the water-ether extract of orychophragmus violaceus has a different lethal effect on roundworms at different concentrations and different treatment times. With the increase of concentration and the prolonging of time, the insecticidal rate is increased. At a concentration of 0.1%, the ascaris lumbricoides can be killed completely within 10 min. At a concentration of 0.5%, the death time is 9min, and the test roundworms can be killed completely. The roundworm killing time is gradually shortened along with the gradual increase of the concentration, and the roundworm can be completely killed only in about 2min when the concentration is 5 percent. From the overall experimental data in table 1, it is seen that the water-ether extract of orychophragmus violaceus has high efficiency and good effect on killing living ascaris.
1.3.2 Effect of February Water-Ether extract on roundworm eggs
The method comprises the steps of respectively using 12 roundworm eggs as experimental objects, treating the roundworm eggs with different concentrations of February extract and different time periods, immediately putting the roundworm eggs back into a culture solution for culture, and detecting whether juvenile roundworm can appear or not so as to judge the killing condition. The results of the experiment are shown in Table 2 below.
TABLE 2
As can be seen from table 2, the water-ether extract of orychophragmus violaceus takes longer to kill roundworm eggs than it takes to kill roundworm bodies, probably because roundworm eggs are more resistant to adverse environmental conditions than the bodies. As the concentration is increased and the time is prolonged, the percentage of killing eggs is increased correspondingly. As can be seen from Table 2, when the concentration is increased to 4%, all the eggs can be effectively killed within 10 min. Therefore, the water-ether extract of orychophragmus violaceus is very quick and effective in killing ascarid eggs.
The experiments prove that the water-ether extract of the orychophragmus violaceus can effectively kill the roundworm bodies and the roundworm eggs, and has the advantages of good killing effect, short action time and low effective concentration.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Various alternatives, modifications and combinations of the features of the invention can be made without departing from the spirit and nature of the invention as claimed, and such simple variations and combinations should also be considered as disclosed in the present application, all falling within the scope of the invention.
Claims (5)
1. The application of the orychophragmus violaceus as an active ingredient in the preparation of a reagent for killing roundworms and/or roundworm ova, wherein the orychophragmus violaceus is a water-ether extract of the whole plant of the orychophragmus violaceus, and the water-ether extract of the whole plant of the orychophragmus violaceus is prepared by the following method:
taking 500-1000g of February herb, mashing, and adding 2000-3000 mL of potassium hydrogen phthalate-sodium hydroxide buffer solution and 20-50mL of vitamin C aqueous solution; standing for 8-16h under a sealed condition; standing, distilling under reflux for 6-8 hr, cooling to room temperature, and filtering to obtain filtrate; extracting the filtrate with diethyl ether for 3-4 times, mixing diethyl ether extractive solutions, and removing diethyl ether from diethyl ether extractive solution in water bath to obtain water-diethyl ether extract of herba Orychophragmae Feddensis.
2. The use according to claim 1, wherein the pH of the potassium hydrogen phthalate-sodium hydroxide buffer is 5.0.
3. The use according to claim 1, wherein the concentration of the aqueous vitamin C solution is 0.2% to 0.5%.
4. An extract of orychophragmus violaceus prepared according to the method of any one of claims 1-3.
5. A method of killing roundworm eggs and/or roundworms in an environment comprising:
applying a water-ether extract of the whole plant of orychophragmus violaceus as claimed in claim 1 to an environment where roundworm eggs and/or roundworms need to be killed; wherein:
when the environment needing to be killed is a dry environment, preparing the water-ether extract of the whole orychophragmus violaceus into an aqueous solution with the concentration of 0.1-5%, and then spraying the aqueous solution into the environment to be killed;
when the environment needing killing is a water body environment, the water-ether extract of the whole plant of the orychophragmus violaceus is directly added into the water body, and the adding amount is 0.1-5% of the dissolved concentration of the orychophragmus violaceus.
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| RU2438688C1 (en) * | 2010-07-05 | 2012-01-10 | Общество С Ограниченной Ответственностью "Акцент" | Anti-helminth medication of vegetable origin |
| CN102382183B (en) * | 2011-11-07 | 2015-09-09 | 四川大学 | The method of antibacterial peptide is extracted from Orychophragmus violaceus |
| CN108740540A (en) * | 2018-07-12 | 2018-11-06 | 全椒县金凤凰禽业养殖专业合作社 | One breeding hen feed plant-source antibacterial expelling parasite additive and preparation method thereof |
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