CN111410565B

CN111410565B - Efficient organic liquid fertilization treatment method for human urine

Info

Publication number: CN111410565B
Application number: CN201910008462.0A
Authority: CN
Inventors: 丁庆
Original assignee: Zhejiang Kingzyme Biotechnology Co ltd
Current assignee: Zhejiang Kingzyme Biotechnology Co ltd
Priority date: 2019-01-04
Filing date: 2019-01-04
Publication date: 2022-04-22
Anticipated expiration: 2039-01-04
Also published as: CN111410565A

Abstract

The invention discloses a high-efficiency treatment method for organic liquid fertilization of human urine, and belongs to the field of human urine treatment. The core of the invention for treating urine is lactobacillus QV1 with the preservation number of CGMCC No.16350, which can be propagated in anaerobic, aerobic and facultative states with high propagation speed. The lactobacillus QV1 is used for inhibiting the growth of bacteria in urine, keeping the urine fresh and preventing the urine from smelling; meanwhile, nutrient substances such as nitrogen, phosphorus and potassium in the urine cannot be decomposed and absorbed by the mixed bacteria; and QV1 can add aeration in the later advanced treatment to convert ammonia nitrogen in urine into nitrate nitrogen; the potassium dihydrogen phosphate enables volatile ammonium carbonate to be converted into ammonium phosphate with stable properties, so that the generation of ammonia gas is prevented in an auxiliary mode, the volatilization loss of nitrogen is prevented, meanwhile, phosphorus and potassium elements in human urine are increased in a proper amount, ammonia is protected by phosphorus, and finally the composite microbial fertilizer is prepared after concentration.

Description

Efficient organic liquid fertilization treatment method for human urine

Technical Field

The invention belongs to the field of urine treatment, and particularly relates to a method for treating organic liquid fertilizer of human urine, which utilizes novel lactobacillus QV1 to treat the human urine.

Background

According to the statistics of the World Health Organization (WHO), the human urine accounts for only 1% of the total domestic sewage in the sewage objects treated by the urban sewage treatment sites of various countries, but 80% of nitrogen, 55% of phosphorus and 63% of potassium treated by the urban sewage treatment sites are derived from the human urine. The pollution (eutrophication) of rivers, lakes and seas mainly comes from the increase of nitrogen and phosphorus, so that human urine is one of the main pollution sources of the natural environment.

On the other hand, human urine is an excellent organic fertilizer, and the contents of nitrogen, phosphorus and potassium are respectively 0.5%, 0.13% and 0.19%, and the organic matter is 2%. According to the latest research, human urine is the only quick-acting fertilizer which can replace chemical fertilizers and is beneficial to soil; energy conservation, environmental protection and resource recycling are advocated in modern society. Therefore, the modern human society urgently needs a complete technology which is used for simply, conveniently and quickly collecting human urine, converting the human urine into organic liquid fertilizer and recycling the organic liquid fertilizer for the modern agriculture.

Human urine is derived from waste fluid from blood filtered by kidney; unlike feces produced through the digestive tract, urine contains no parasite eggs, and urine immediately discharged by healthy persons is essentially sterile; human urine is 95% water, 5% soluble substances and inorganic salts, of which about 2% is urea, 1% is sodium chloride, and small amounts of uric acid, hippuric acid, creatinine, oxalic acid, sulfate, nitric acid, xanthine and the taste substances androsterone. Therefore, the urine of normal people is fully filtered by the kidney, is safe and sanitary, can be obtained everywhere and has no raw material cost; the key is to identify simple and low cost means of collection and on-site pretreatment. Although there are reports on the treatment of urine at present, there is no good method.

For example, chinese patent application No. 201510243284.1, published as 2015, 11/11, discloses a method for treating urine from a farm animal, wherein the urine is discharged by feeding the farm animal with water treated with a glass composition. Actually, urine itself has no odor, and is rich in nutrients such as protein, inorganic salts, organic matters and the like, so that bacteria are easy to breed, and urea is decomposed to generate ammonia odor. The method of this patent does not solve the fundamental problem of urine odor. Chinese patent application No. 201510047504.3, application publication No. 2015, 4-29 discloses a method for treating urine. Which comprises the following steps: taking a plurality of raw urine for standby; adding urease to remove urea; adding an internal standard and fixing the volume; adjusting the pH value, adding hydroxylamine hydrochloride, and neutralizing after reaction; extracting, drying and then carrying out derivatization reaction; the retention time calibration is performed. The method disclosed in the patent is used for pretreatment of urine, aims to ensure that the detection in the later period is more accurate, and uses urease, so that the cost is higher, and the method is not suitable for wide application. More importantly, the existing method only treats urine to achieve the purpose of no odor or emission, and the urine, a valuable fertilizer resource, is not recycled.

At present, the market needs a method which can effectively prevent the odor of urine, does not lose or hardly lose effective nutrient components such as nitrogen, phosphorus and potassium in the urine and prepares the nutrient components into organic liquid fertilizer so as to completely recycle the nutrient components.

Disclosure of Invention

1. Problems to be solved

Aiming at the problem that the existing urine cannot be effectively treated, the invention aims to provide a urine treatment method, wherein the used lactobacillus can inhibit the growth of other mixed bacteria and keep the urine fresh.

One object of the present invention is to provide a method for treating urine, which uses lactobacillus with high reproductive capacity, and can rapidly propagate in urine to prevent the growth of bacteria in urine, thereby preventing the bacteria from decomposing urea in urine into ammonia, and preventing urine from smelling.

The invention also aims to provide a urine treatment method, which utilizes the lactobacillus to prevent the growth of mixed bacteria, and utilizes the monopotassium phosphate to convert volatile ammonium carbonate into ammonium phosphate with stable property, thereby preventing the generation of ammonia gas and the volatilization loss of nitrogen, simultaneously increasing a proper amount of phosphorus and potassium elements in human urine, protecting ammonia with phosphorus, and finally concentrating to prepare the composite microbial fertilizer.

A third object of the invention is: after the fresh and odorless urine is collected in various toilets, the urine is subjected to concentrated advanced treatment to concentrate nitrogen, phosphorus and potassium nutrients stored in the urine, and simultaneously, the main existing form of nitrogen is converted from ammonia nitrogen into nitrate nitrogen, so that the nitrogen becomes a liquid organic fertilizer which is most easily absorbed by plants.

2. Technical scheme

In order to solve the problems, the technical scheme adopted by the invention is as follows:

a urine treatment method uses lactobacillus QV1 as a treating agent for treatment, wherein QV1 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 16350.

Furthermore, the viable count of the lactobacillus QV1 is 10⁷～10¹¹In the cfu/g range, the addition amount of the lactobacillus QV1 is 0.01-50% of the mass of the urine.

Furthermore, the viable count of the lactobacillus QV1 is 10⁷～10¹¹In the cfu/g range, said milkThe addition amount of the bacillus QV1 is preferably 0.1-20% of the mass of urine.

Furthermore, the viable count of the lactobacillus QV1 is 10⁹The addition amount of the lactobacillus QV1 is preferably 1-5% of the mass of urine at cfu/g.

Further, potassium dihydrogen phosphate was added while treating the urine with lactobacillus QV 1.

Furthermore, the adding amount of the potassium dihydrogen phosphate is 0.5-10% of the mass of the urine.

A method of treating human urine comprising the steps of:

(1) collecting fresh human urine (feces-urine separated toilets, urinals, etc.);

(2) adding lactobacillus QV1 powder into a storage barrel of the collected urine, stirring uniformly, and storing in a sealed manner;

(3) after collection, the obtained product is deeply processed in a liquid fertilizer factory, an aeration device is used for proper aeration, and the high-efficiency nitrification function of QV1 contained in urine under aerobic conditions is utilized to convert ammonia nitrogen components stored in the urine into nitrate nitrogen. Nitrate nitrogen is the most easily absorbed nitrogen fertilizer form by plants, and the treatment can reduce the existence of nitrite nitrogen to the maximum extent and ensure the safety of food.

Further, potassium dihydrogen phosphate is added simultaneously in the step (2).

Furthermore, the urine in the step (2) is stored for 5-7 days in a sealing way.

Further, concentrating the urine treated in the step (2) or (3) by 2-3 times to prepare the compound microbial fertilizer.

An organic liquid fertilizer is prepared from human urine as raw material and lactobacillus QV1 as carrier.

Furthermore, the human urine is used as a raw material and is obtained after being treated by any one of the methods.

A urine treatment agent comprises lactobacillus QV1 and an acceptable carrier, wherein lactobacillus QV1 is an active ingredient.

The Lactobacillus is separated from the inner Mongolia grassland pasture, named as strain QV1, the microbial research institute of Chinese academy of sciences detects the censored strain, and the Lactobacillus sp. ((2018) micro-indexing No. 334).

The lactic acid bacteria are detected and identified as new lactic acid bacteria,

biological material preservation information

The Lactobacillus QV1 is classified as Lactobacillus paracasei and Lactobacillus paracasei, and is preserved in the China general microbiological culture Collection center, the preservation address is No. 3 Xilu No.1 Hospital of the North district, Chaoyang district, Beijing city, the preservation date is 8 months and 28 days in 2018, and the preservation number is CGMCC No. 16350.

3. Advantageous effects

Compared with the prior art, the invention has the beneficial effects that:

(1) according to the urine treatment method provided by the invention, the growth of mixed bacteria in urine is inhibited by using the lactobacillus QV1, the freshness of the urine is kept, and the urine is prevented from smelling.

(2) According to the urine treatment method provided by the invention, the lactobacillus QV1 is matched with potassium dihydrogen phosphate for use, the QV1 bacterial powder and the potassium dihydrogen phosphate can keep human urine in a fresh state so as to control odor generation and nitrogen and other nutrient substances to be decomposed and absorbed by other bacteria, and the potassium dihydrogen phosphate enables volatile ammonium carbonate to be converted into ammonium phosphate with stable properties, so that the generation of ammonia gas and the volatilization loss of nitrogen are prevented, meanwhile, a proper amount of phosphorus and potassium elements in the human urine are increased, the ammonia is protected by phosphorus, and finally, the composite microbial fertilizer is prepared after concentration.

(3) According to the urine treatment method provided by the invention, the lactobacillus QV1 can prevent generation of calculus in urine.

Drawings

FIG. 1 is a schematic view of a urine advanced treatment process according to the present invention.

Detailed Description

The invention is further described with reference to specific examples.

The lactobacillus QV1 is a brand new separated strain. Relevant biological activity experiments show that the lactobacillus QV1 is a strong acid-resistant and high-salinity-resistant strain, has good high-temperature resistance, can decompose oil and fat substances, and can be applied to a plurality of fields such as sewage treatment agents, urine treatment agents, livestock and poultry feed additives, foods, health care products, medicines and the like.

The invention provides an application of lactobacillus QV1 in preparing sewage treatment agents and urine treatment agents, which is characterized in that lactobacillus QV1 and calcium carbonate and other auxiliary materials are pressed into fungus cakes to be matched with potassium dihydrogen phosphate for use, the urine can be kept in a fresh state to control odor generation and nitrogen and other nutrient substances to be decomposed and absorbed by mixed bacteria, the potassium dihydrogen phosphate enables volatile ammonium carbonate to be converted into ammonium phosphate with stable property, thereby preventing ammonia generation and nitrogen volatilization loss, simultaneously increasing phosphorus and potassium elements in the urine in a proper amount to protect ammonia with phosphorus, and finally the treated urine can be prepared into compound microbial fertilizer for sale after being concentrated.

The present invention will be described in further detail with reference to specific examples. The following examples are merely illustrative of the present invention and should not be construed as limiting thereof.

Example 1

QV1 bacterial powder, calcium carbonate, maltodextrin and other substances are made into bacterial cakes (QV 1 bacterial powder is fully mixed with calcium carbonate powder below 800 meshes, excipients such as maltodextrin are added, and then the mixture is circularly pressed according to the size of a drain opening of a urinal) and is placed at an inlet of the urinal (namely a urine collecting opening), the QV1 viable bacteria can be dissolved in a small amount in each urine irrigation bacterial cake and flow into a collecting container after flowing through a pipeline, so that the QV1 viable bacteria with the effect of a natural preservative are uniformly distributed in the urine irrigation bacterial cake from the urinal to the collecting container, other miscellaneous bacteria are uninterruptedly inhibited, the urine cannot be decomposed to generate ammonia gas (ammonia odor) and carbon dioxide, and stone components in the urine can be dissolved and removed. VBT filler (special continuous foaming special ceramic filler) is used in the urine treatment process, so that the proliferation speed of QV1 can be increased, and loss is prevented.

The main objectives of on-site urine pretreatment are: 1) removing odor; 2) inhibiting the environmental infectious microbes from decomposing nitrogen components in the urine, completely preserving the nitrogen components in the urine as much as possible, and keeping the total nitrogen content in the urine as much as possible; 3) after being stored on site for 5-10 days, the concentrated liquid can be naturally evaporated and concentrated to reduce the urine volume and improve the proportion of effective nutrient substances (nitrogen, phosphorus and potassium); 4) controlling the biochemical reaction process and viable count of microorganisms, and keeping the pH value within 5.5-8.

The pretreated urine in each public toilet is collected and concentrated in a deep processing plant, and after deep processing (aerobic treatment for converting ammonia nitrogen into nitrate nitrogen, concentration, adjustment of nutrient components and the like), the urine reaches the national standard of the compound microbial fertilizer, and the product is packaged.

Example 2

A method of treating urine comprising the steps of:

(1) collecting fresh urine;

(2) adding lactobacillus QV1 powder into the collected urine, wherein the viable count of lactobacillus QV1 is 10⁷～10¹¹In the cfu/g range, the adding amount is 0.01-50% of the mass of the urine, and the preferable adding amount is 0.1-20%; the viable count of the lactobacillus QV1 is 10⁹When cfu/g is adopted, the more preferable addition amount is 1-5%, and the best addition amount is 1%; meanwhile, 2-3% of monopotassium phosphate is added, the mixture is uniformly stirred and stored in a sealed mode for 5-7 days, and QV1 bacteria powder and the monopotassium phosphate can keep human urine in a fresh state so as to control odor generation and nitrogen and other nutrient substances to be decomposed and absorbed by other bacteria.

The urea in human urine gradually reacts with water in aqueous solution to form ammonium carbonate:

CO(NH₂)₂+2H₂O─→(NH₄)₂CO₃；

after 2% -3% of monopotassium phosphate is added:

(NH₄)₂CO₃+KH₂PO₄─→(NH₄)₃PO₄+KHCO₃

thus, the volatile ammonium carbonate can be converted into ammonium phosphate with stable property, thereby preventing the generation of ammonia gas and the volatilization loss of nitrogen, and simultaneously, the phosphorus and potassium elements in human urine are increased in a proper amount to protect ammonia by phosphorus;

(NH₄)₃PO₄and KHCO₃The fertilizer is easy to be absorbed by plants as nutrients in a liquid state, and the effect of high-efficiency liquid fertilizer is achieved.

The on-site fresh-keeping urine is inspected after aerobic treatment (aeration treatment) for 10 days.

Finally concentrating by 2-3 times to prepare a finished product, and packaging the finished product after the inspection reaches the national standard of the compound microbial fertilizer;

refer to Table 1 below (the test report of the unconcentrated human urine of the test of Zhejiang academy of agricultural sciences).

When the organic bacterial fertilizer is used specifically, water is added according to the proportion of 1: 200-1: 500 for dilution, and then vegetables, fruits and vegetables are irrigated.

TABLE 1 organic bacterial manure test report obtained in this example

As the above test reports indicate: after QV1 and potassium dihydrogen phosphate treatment, the unconcentrated state reaches the national standard of the microbial compound bacterial fertilizer (refer to the national standard), and after concentration, the unconcentrated state can completely reach the national standard.

Advanced treatment after collection of on-site fresh-keeping urine

Collecting the fresh-keeping urine treated by QV1 and potassium dihydrogen phosphate into a factory, placing the urine in an original urine tank, carrying out advanced treatment according to the following process, wherein a micro-nano aeration device VSJ60/0.75KW is used for aeration, three continuous aeration tanks use bottom aeration discs, and weak continuous aeration (the aeration tanks use a 1.5KW fan together); conveying the urine after aeration treatment to a settling tank for settling, wherein the reflux ratio of the settling tank to the original urine tank is 100-150%; all add the heat preservation area in the three continuous concentrator bowl, the constant temperature is at 40 degrees, uses aeration dish aeration simultaneously (last concentrator bowl uses micro-nano aeration device VSJ80 (join in marriage 1KW immersible pump) strong aeration, and preceding two concentrator bowl are with bottom aeration dish, a 1.5KW fan of sharing), and the top negative pressure is taken out vapour, and the target is concentrated the urine 2-3 times and is obtained liquid fertilizer. It is noted here that a weaker aeration is required in the aeration tank and maintained for a longer time, which allows a higher conversion of ammonia nitrogen into nitrate nitrogen, while at the same time minimizing denitrification (which results in nitrogen removal and thus nitrogen nutrient reduction).

Through the deep aerobic treatment, phosphorus and potassium components in the obtained organic liquid fertilizer product are basically preserved, and ammonia nitrogen components are basically converted into nitrate nitrogen (nitrate) components which become nitrogen nutrients most easily absorbed by plants.

The deep processing method avoids the generation of a large amount of nitrite caused by incomplete oxidation in the common solid manure composting method, and the nitrite can enter crop vegetables through farmyard manure compost and then enter human bodies. Nitrite is a typical carcinogen and recent international research has found that it binds to hemoglobin, reducing the speed of blood flow and nutrient carrying capacity. Nitrite is difficult to metabolize by humans, thus endangering the health and longevity of humans or animals.

Example 3

Rapid propagation and revival experiments

100 mg of QV1 lyophilized powder was put into a beaker containing 20 ml of 35-40 ℃ warm water, and other lactobacilli were added under the same conditions (typical LBF128 lactobacilli was used as a control). LBF128 starts fermentation to become acidic after 30 minutes, while QV1 starts fermentation to become acidic after 5 minutes. The recovery and the propagation speed are obviously superior to those of the common lactobacillus.

Example 4

Acid resistance test: lyophilized bacterial powder (10) of QV1 was used¹¹cfu/g), diluted 100 times with warm water at 37 ℃ and left for 0.5 hour, MRS liquid media adjusted to pH 3.0 and 2.0 (using hydrochloric acid) were placed, and viable cell counts were measured after 1 hour and 2 hours, respectively, with the following results:

pH value	Before testing	1 hour after addition of strong acid	2 hours after the addition of the strong acid
				pH 3.0	5*10(8)	4*10(8)	6*10(7)
pH 2.0	5*10(8)	6*10(7)	5*10(6)

Note: in the above table, 5 × 10(8) means 8 th power of viable bacteria multiplied by 5 by 10, and the same applies to the rest.

Example 5

Salt tolerance test: lyophilized bacterial powder (10) of QV1 was used¹¹cfu/g), diluted 100 times with warm water at 37 ℃ and left for 0.5 hour, MRS liquid media with bile salt concentration adjusted to 0.1% and 0.3% were added, and viable cell count was measured after 1 hour and after 2 hours, respectively, with the following results:

	before testing	1 hour after adding bile salt	2 hours after the addition of bile salts
				0.1 percent of bile salt	5*10(8)	3.5*10(8)	2*10(8)
0.3 percent of bile salt	5*10(8)	2*10(7)	1.2*10(6)

Example 6

QV1 lyophilized powder (10) for high temperature resistance test¹¹cfu/g), diluted 100 times with warm water at 37 ℃ and left for 0.5 hour, put MRS liquid culture medium heated to 80 ℃ and 100 ℃ respectively, and the viable count was measured after 10 minutes and after 30 minutes, respectively, with the following results:

	before testing	After 1 hour	After 2 hours
				80 ℃ hot water	5*10(8)	4.5*10(8)	2.5*10(8)
100 ℃ hot water	5*10(8)	1*10(8)	2*10(7)

Claims

1. A method of treating human urine comprising the steps of:

(1) collecting fresh human urine;

(2) adding lactobacillus QV1 strain powder and potassium dihydrogen phosphate into the collected human urine, uniformly stirring, and storing for 5-7 days in a sealed manner; the QV1 strain is preserved in the China general microbiological culture Collection center with the preservation number of CGMCC No. 16350; the addition amount of the lactobacillus QV1 is 0.01-50% of the mass of human urine, and the viable count of the lactobacillus QV1 is 10⁷~10¹¹cfu/g range timepieces; the adding amount of the monopotassium phosphate is 0.5-10% of the mass of the urine;

(3) and (3) carrying out aeration treatment on the urine hermetically stored in the step (2), and concentrating the treated urine by 2-3 times to prepare the compound microbial fertilizer.

2. The human urine processing method as claimed in claim 1, wherein the amount of the added lactobacillus QV1 is 1-5% of the urine by weight, and the viable count of the lactobacillus QV1 is 10⁹cfu/g are measured.

3. An organic liquid fertilizer, which is characterized by being obtained by using human urine as a raw material and treating the human urine by the method of any one of claims 1 to 2.