CN114292527B - Method for extracting maize yellow component from maize - Google Patents
Method for extracting maize yellow component from maize Download PDFInfo
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- CN114292527B CN114292527B CN202111620610.8A CN202111620610A CN114292527B CN 114292527 B CN114292527 B CN 114292527B CN 202111620610 A CN202111620610 A CN 202111620610A CN 114292527 B CN114292527 B CN 114292527B
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Abstract
The invention provides a method for extracting a maize yellow component from maize or maize processing byproducts, which utilizes a mixed solution of a green lipophilic eutectic solvent and water as an extraction solvent, thereby overcoming the defects of large organic solvent consumption, toxic organic solvent residue and denaturation of maize yellow components caused in the process of extracting or heating to recover the solvent for a long time in the traditional extraction method. The invention utilizes the mixed solution of lipophilic eutectic solvent and water as the extraction solvent, utilizes the normal temperature extraction technology, realizes the high-efficiency extraction of the carotenoid in the corn waste residue, and overcomes the defects of large organic solvent consumption, toxic organic solvent residue and carotenoid denaturation caused by long-term heating process in the traditional extraction method. In addition, the corn gluten extracted by the method can be directly used for adding food and cosmetic products, does not need a complex solvent recovery process, and provides a feasible scheme for green and high-efficiency preparation of carotenoid products.
Description
Technical Field
The invention belongs to the technical field of natural product extraction, and particularly relates to a method for extracting a maize yellow component from maize or maize processing byproducts.
Background
Corn gluten is a natural pigment extracted from corn or corn gluten meal which is a by-product of corn starch processing. It is carotenoid pigment, and contains zeaxanthin, xanthophyll, etc. as main ingredients. The corn yellow has effects of resisting oxidation, preventing macular degeneration, treating cataract, preventing cardiovascular disease, enhancing immunity, and relieving atherosclerosis. In the food industry, carotenoids such as zeaxanthin and lutein are gradually replacing synthetic pigments such as lemon yellow and sunset yellow as natural food pigments, and in 1996, zeaxanthin is listed as one of natural pigments that are allowed to be added for eating. In the national food additive standard for food safety (GB 2760-2014), the maximum addition amount of the zeaxanthin is 5.0g/kg, so that the zeaxanthin as a functional component has a wide market prospect, and the extraction research of the zeaxanthin is gradually hot.
The traditional extraction method of the maize yellow is to repeatedly soak and extract by using ethanol or petroleum ether, collect soak solution and carry out vacuum concentration to obtain the maize yellow. However, since the corn gluten is generally tightly bound to the protein of corn in corn or corn by-products, the corn gluten cannot be directly exposed, and is difficult to contact with an organic solvent, resulting in low extraction efficiency, long extraction time and denaturation of the corn gluten. In addition, the organic solvent residue in the corn steep liquor is easily out of the requirement of the food additive due to the use of a large amount of organic solvent. In addition, zeaxanthin has the disadvantages of poor stability, insolubility in water, and easy decomposition, which limits its application in the food and cosmetic industries.
Disclosure of Invention
The invention provides a method for extracting a maize yellow component from maize or maize processing byproducts, which utilizes a mixed solution of a green lipophilic eutectic solvent and water as an extraction solvent, thereby overcoming the defects of large organic solvent consumption, toxic organic solvent residue and denaturation of maize yellow components caused in the process of extracting or heating to recover the solvent for a long time in the traditional extraction method.
The invention provides a method for extracting a corn yellow component from corn or a corn processing byproduct, which comprises the following steps:
1) Drying corn or corn by-products, and crushing to prepare corn flour;
the drying is to ensure that the moisture content is less than 10 percent;
2) Putting corn flour into an extraction tank connected with a pipeline type continuous high-shear homogenizer, wherein the rotating speed is 3000-22000rpm, and the flow rate is adjusted to 50-1000L/h;
3) Adding a mixture solvent of an organic solvent and water as an extraction solvent into an extraction tank, wherein the extraction temperature is 25-40 ℃;
4) After extraction, standing and centrifuging to obtain an upper phase solution containing the maize yellow;
5) Adding water into the upper phase solvent, fully mixing, centrifuging, and layering to obtain an upper phase solvent, namely the corn yellow extract.
The ratio of the eutectic solvent to water is 3.
The feed-liquid ratio of the corn flour to the solvent is 1:3-1:10 (mass/volume ratio)
One of the extraction solvents comprises oleic acid, caprylic acid and water or menthol, acetic acid and water;
wherein the volume ratio of oleic acid, caprylic acid and water is 1; and the molar ratio of oleic acid to octanoic acid is 1.
The invention utilizes the mixed solution of lipophilic eutectic solvent and water as the extraction solvent, utilizes the normal temperature extraction technology, realizes the high-efficiency extraction of the carotenoid in the corn waste residue, and overcomes the defects of large organic solvent consumption, toxic organic solvent residue and carotenoid denaturation caused by long-term heating process in the traditional extraction method. In addition, the corn gluten extracted by the method can be directly used for adding food and cosmetic products, does not need a complex solvent recovery process, and provides a feasible scheme for green and high-efficiency preparation of carotenoid products.
Drawings
FIG. 1: a comparison graph of liquid chromatogram of the low eutectic solvent extract and ethanol extract under the same conditions in example 1;
FIG. 2 is a schematic diagram: comparative graph of liquid chromatogram of low eutectic solvent extract and petroleum ether extract under the same conditions in example 1.
Detailed Description
According to the invention, the corn protein substances in the corn flour are quickly crushed by virtue of pipeline continuous high-shear homogenization; the protein substances can be dissolved by the prepared extraction solvent, and the wrapped maize yellow components are quickly released and dissolved, so that the maize yellow components (zeaxanthin and lutein) are efficiently extracted at normal temperature.
The present invention will be described in detail with reference to examples.
Example 1:
1) Drying the corn by-product after preparing the corn starch by using blast air to ensure that the moisture content of the corn by-product reaches 8 percent, and crushing the corn by-product to prepare the corn flour.
2) 500g of corn flour was put into a 10L experimental extraction tank connected to an inline continuous high shear homogenizer.
3) Add 1.25L oleic acid: mixing octanoic acid (1,mol/mol) and 2.5L water, and adjusting the rotation speed of the pipeline type continuous high-shear homogenizer to 22000rpm and the flow rate to 50L/h; circularly extracting at room temperature for 10min;
4) Standing after extraction, centrifuging at 4000rpm for 10min to separate the extractive solution into two phases, and collecting eutectic solvent phase containing zeaxanthin component;
5) Adding 1.25L water into the eutectic solvent phase extraction solution, mixing, standing, centrifuging, and discarding the water solution. Repeat 2 times. The upper phase solvent is corn yellow extract, and can be directly used as food additive.
Example 2:
1) Drying corn with blast air until the water content reaches 6%, and pulverizing to obtain corn flour.
2) 5kg of corn flour was put into a 100L experimental extraction tank connected to an in-line continuous high shear homogenizer.
3) Adding a mixed extraction solvent of 25L of menthol, acetic acid (1,mol/mol) and 25L of water into an extraction tank, adjusting the rotation speed of an in-line continuous high-shear homogenizer to 10000rpm, and adjusting the flow rate to 400L/h; circularly extracting at room temperature for 30min;
4) Standing after extraction, pumping into a tube centrifuge with the rotation speed of 16000rpm to separate the extract into two phases, and taking a eutectic solvent phase containing the zeaxanthin component;
5) Adding 25L water into the eutectic solvent phase extraction solution, mixing, standing, centrifuging, and discarding the water solution. Repeat 2 times. The upper phase solvent is corn yellow extract, and can be directly used as additive of cosmetics.
Example 3:
under the same conditions, the extraction efficiency of the extraction method of the invention in example 1 is compared with that of the traditional extraction solvent ethanol and petroleum ether.
The extraction method of ethanol and petroleum ether comprises the following steps:
1) Drying the corn by-product after preparing the corn starch by blast drying to make the water content reach 8%, and crushing to prepare the corn flour.
2) 500g of corn flour was put into a 10L experimental extraction tank connected to an inline continuous high shear homogenizer.
3) Adding 4L of ethanol or petroleum ether into the extraction tank, adjusting the rotation speed of the pipeline type continuous high-shear homogenizer to 22000rpm, and adjusting the flow rate to 50L/h; circularly extracting at room temperature for 10min;
4) Standing after extraction, centrifuging at 4000rpm for 10min, and collecting supernatant to obtain ethanol or petroleum ether extract of corn yellow;
5) Recovering solvent from ethanol or petroleum ether extractive solution of corn yellow to 1.25L under reduced pressure to obtain extract solution with same extraction conditions as eutectic solvent extract.
Respectively detecting the content of zeaxanthin and lutein in the three extraction solvents by using HPLC, wherein the HPLC chromatographic conditions are as follows:
YMC C30 (4.6 mm. Times.250mm, 5 μm); the detection wavelength is 455nm; the injection volume is 20 mu L;
the composition of mobile phase a and mobile phase B was as follows:
Time | mobile phase A | Mobile phase B |
0 | 98% | 2% |
11 | 98% | 2% |
18 | 60% | 40% |
24.5 | 60% | 40% |
27 | 0 | 100% |
FIGS. 1 and 2 are comparative images of liquid chromatograms of the solvent used in example 1 and ethanol and petroleum ether extracts under the same conditions, respectively. As can be seen from the figure, the extraction rate of lutein and zeaxanthin when extracted by the method of example 1 is 35.77% and 43.49%, respectively; when the ethanol is extracted, the extraction rates of the lutein and the zeaxanthin are respectively 25.32 percent and 27.65 percent; when petroleum ether is extracted, the extraction rates of the lutein and the zeaxanthin are 7.47 percent and 5.23 percent respectively. The result shows that the method of the invention is obviously superior to the traditional organic solvent extraction effect.
Claims (5)
1. A method for extracting a zeaxanthin component from corn or corn processing by-products, comprising the steps of:
1) Drying corn or corn by-products, and crushing to prepare corn flour;
2) Putting corn flour into an extraction tank connected with a pipeline type continuous high-shear homogenizer for treatment;
3) Adding a mixture solvent of an organic solvent and water as an extraction solvent into an extraction tank for extraction;
the volume ratio of the organic solvent to the water is 1;
4) After extraction, standing and centrifuging to obtain an upper phase solution containing the maize yellow;
5) Adding water into the upper phase solvent, fully mixing, centrifuging, and demixing to obtain an upper phase solvent, namely the maize yellow component.
2. The method of claim 1, wherein the drying in 1) is carried out to a moisture content of less than 10%.
3. The method as set forth in claim 1, wherein the rotation speed of the treatment in 2) is 3000-22000rpm, and the flow rate is adjusted to 50-1000L/h.
4. The method of claim 1, wherein the mass to volume ratio of corn meal to extraction solvent in 3) is 1:3-1: 10.
5. the method of claim 1, wherein the extraction temperature of step 3) is 25-40 ℃.
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Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108299265A (en) * | 2018-01-17 | 2018-07-20 | 济宁医学院 | A method of extracting zeaxanthin and lutein from corn and its by-product |
CN108586306A (en) * | 2018-05-23 | 2018-09-28 | 华东理工大学 | A method of lutein and zeaxanthin are detached using ionic liquid strengthening extraction |
CN108893185A (en) * | 2018-07-26 | 2018-11-27 | 河西学院 | The method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease |
CN111153843A (en) * | 2020-01-10 | 2020-05-15 | 中国科学院兰州化学物理研究所 | Method for separating lycium barbarum pigment monomers |
CN111675640A (en) * | 2020-07-27 | 2020-09-18 | 中国科学院兰州化学物理研究所 | Method for separating and preparing high-purity zeaxanthin |
CN113509751A (en) * | 2021-07-13 | 2021-10-19 | 武汉大学 | Extraction-back extraction system based on alkanol eutectic solvent and application thereof |
CN113603567A (en) * | 2021-06-09 | 2021-11-05 | 华南农业大学 | Method for selectively extracting active ingredients of magnolia bark dregs and co-producing biomethane |
CN113735802A (en) * | 2021-10-12 | 2021-12-03 | 自然资源部第三海洋研究所 | A method for preparing fucoxanthin extract with eutectic solvent |
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Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108299265A (en) * | 2018-01-17 | 2018-07-20 | 济宁医学院 | A method of extracting zeaxanthin and lutein from corn and its by-product |
CN108586306A (en) * | 2018-05-23 | 2018-09-28 | 华东理工大学 | A method of lutein and zeaxanthin are detached using ionic liquid strengthening extraction |
CN108893185A (en) * | 2018-07-26 | 2018-11-27 | 河西学院 | The method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease |
CN111153843A (en) * | 2020-01-10 | 2020-05-15 | 中国科学院兰州化学物理研究所 | Method for separating lycium barbarum pigment monomers |
CN111675640A (en) * | 2020-07-27 | 2020-09-18 | 中国科学院兰州化学物理研究所 | Method for separating and preparing high-purity zeaxanthin |
CN113603567A (en) * | 2021-06-09 | 2021-11-05 | 华南农业大学 | Method for selectively extracting active ingredients of magnolia bark dregs and co-producing biomethane |
CN113509751A (en) * | 2021-07-13 | 2021-10-19 | 武汉大学 | Extraction-back extraction system based on alkanol eutectic solvent and application thereof |
CN113735802A (en) * | 2021-10-12 | 2021-12-03 | 自然资源部第三海洋研究所 | A method for preparing fucoxanthin extract with eutectic solvent |
Non-Patent Citations (1)
Title |
---|
玉米黄浆中玉米黄色素的提取工艺研究;解耸林;《食品科技》;20110520;第36卷(第05期);233-236 * |
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