CN108893185A - The method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease - Google Patents
The method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease Download PDFInfo
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- CN108893185A CN108893185A CN201810836281.2A CN201810836281A CN108893185A CN 108893185 A CN108893185 A CN 108893185A CN 201810836281 A CN201810836281 A CN 201810836281A CN 108893185 A CN108893185 A CN 108893185A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/108—Production of fats or fatty oils from raw materials by extracting after-treatment, e.g. of miscellae
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- Oil, Petroleum & Natural Gas (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
- Extraction Or Liquid Replacement (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method includes will have the microalgae algae mud of certain water content to be added in flash extracter, certain time is extracted in the case where centainly extracting voltage, obtain the algae slurries containing microalgae grease, algae slurries and magnetic hydrophobic eutectic solvent are mixed well, it stands, separate magnetic hydrophobic eutectic solvent phase, the hydrophobic eutectic solvent of magnetropism is added to bar magnet, recycle magnetic hydrophobic eutectic solvent, obtain microalgae grease, this method has extraction time short, it is high-efficient, low power consumption and other advantages；It can realize that microalgae grease effectively extracts using magnetic hydrophobic eutectic solvent, under the action of an external magnetic field, reach the separation purpose of magnetic hydrophobic eutectic solvent and grease, to realize that magnetic hydrophobic eutectic solvent recycles.
Description
Technical field
The invention belongs to the separation technology field of microalgae grease, in particular to a kind of magnetic hydrophobic eutectic solvent auxiliary is dodged
The method that formula extraction prepares microalgae grease.
Background technique
Microalgae because the advantages that fat content is high, cultivation cycle is short, condition of culture is easy to control and sustainable production have become it is good
Good biomass resource research object.However, microalgae from culture, harvest, extract that be converted into biodiesel be a high cost
Investment process, it has also become the important obstruction of microalgae research and development.In oil extraction processes, clasmatosis, extraction need to persistently disappear
Organic solvent and energy are consumed, therefore takes effective extracting method to shorten extraction time, reduces organic solvent consumption and drop
Low energy output, while ensuring that effective extraction effect has become important reduction cost input link.
Homogenate extraction method is a kind of extracting method emerging in recent years, at room temperature, can be by material quick crashing to appropriate
Granularity, and effective component infiltration is discharged with vibration by quickly stirring, there are the spies such as extraction time is short, high-efficient, low energy consumption
Point.
Compared with conventional organic solvents and ionic liquid, eutectic solvent be by certain stoichiometric ratio hydrogen bond receptor and
Hydrogen-bond donor is composed, and is had many advantages, such as low melting point, low cost, hypotoxicity, is easily prepared, can regenerate, is biodegradable.It is hydrophilic
Property eutectic solvent is widely used to flavones, active constituent mentions in polyphenol and other biomass as a kind of extraction medium
It takes and separates；Currently, the application study in relation to hydrophobic eutectic solvent is relatively fewer, it is mainly used in the extraction of Model Molecule；
Prior art discloses use hydrophobicity eutectic solvents to extract polypren acetic acid esters in ginkgo leaf, and is returned using macroreticular resin
Receive target product, but there are the target product rate of recovery is not high, it is cumbersome the defects of；The prior art has to be mentioned using eutectic solvent
The report of microalgae grease is taken, but used eutectic solvent recovery rate is low, and realizes eutectic solvent and target product
Separation process it is cumbersome.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of new magnetic hydrophobic eutectic solvents to assist homogenate extraction
The method for preparing microalgae grease, this method is easy to operate, and extraction effect is good, green solvent recoverable.
Specific technical solution of the present invention is as follows：
The present invention provides a kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method
Include the following steps：
1) it will be added in flash extracter, extracted in the case where centainly extracting voltage certain with the microalgae algae mud of certain water content
Time obtains the algae slurries containing microalgae grease；
2) the algae slurries containing microalgae grease prepared by step 1) are mixed well with magnetic hydrophobic eutectic solvent, are stood,
Separate magnetic hydrophobic eutectic solvent phase；
3) bar magnet is added in the magnetic hydrophobic eutectic solvent phase separated to step 2), recycling magnetic hydrophobic eutectic is molten
Agent obtains microalgae grease.
Preferably, the method for the microalgae bath mud with certain water content is：Growth logarithmic phase microalgae is taken, is obtained after filtering
Algal gel adds water oscillation to be suspended into water content and reaches specified content.
Further to improve, microalgae is to have chlorella, chrysophyceae, micro- quasi- ball algae, scenedesmus etc..
Further to improve, magnetic hydrophobic eutectic solvent described in step 2) is that molar ratio is 1:1-2:1 DL- peppermint
The mixture of alcohol, organic acid and anhydrous ferric trichloride.
Further to improve, the organic acid is selected from one of acetic acid, pyruvic acid, levulic acid or lactic acid or a variety of；
Preferably, the magnetic hydrophobic eutectic solvent is molar ratio 1:1:1 DL- menthol, acetic acid and anhydrous ferric trichloride it is mixed
It closes object or molar ratio is 1:2:1 DL- menthol, the mixture of pyruvic acid and anhydrous ferric trichloride or molar ratio are 1:1:1
DL- menthol, levulic acid and anhydrous ferric trichloride mixture or molar ratio be 1:2:1 DL- menthol, lactic acid and
The mixture of anhydrous ferric trichloride.
Further to improve, the water content of microalgae bath mud is 20%-85% in step 1), it is preferable that water content 40%-
75%.
Further to improve, it is 40-80V that voltage is extracted in step 1), it is preferable that extraction voltage is 50-70V.
Further to improve, extraction time is 0.5-3min in step 1), it is preferable that extraction time 1-2.5min.
Further to improve, algae slurries and the volume ratio of magnetic hydrophobic eutectic solvent in step 2) containing microalgae grease are
1:0.3-1, it is preferable that volume ratio 1:0.5-1:1.
It is further to improve, time of repose 0.5-2h, it is preferable that time of repose 0.7-1.5h.
Further to improve, the magnetism of bar magnet is 0.5-1.5T in step 3).
Compared with prior art, a kind of magnetic hydrophobic eutectic solvent auxiliary homogenate extraction provided by the present invention is prepared micro-
The advantages of method of algae oil rouge, is：The homogenate extraction that the present invention uses passes through with quick crashing material to appropriate granularity
Quickly stirring and vibration accelerate microalgae grease infiltration release, have that extraction time is short, high-efficient, low power consumption and other advantages；Using magnetic
The hydrophobic eutectic solvent of property can realize that microalgae grease effectively extracts, and under the action of an external magnetic field, it is molten reach magnetic hydrophobic eutectic
The separation purpose of agent and grease, to realize that magnetic hydrophobic eutectic solvent recycles, and by magnetic hydrophobic eutectic solvent
The recovery rate of microalgae grease is significantly improved in conjunction with homogenate extraction method.
Specific embodiment
Embodiment 1
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 50% water content is added in flash extracter, extracts 1min in the case where 50V extracts voltage,
Obtain the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.5 mixing, sufficiently
It mixes, stands 0.7h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:1:1 DL-
The mixture of menthol, acetic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 0.6T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 98.42%.
It should be noted that recovery rate=microalgae grease weight/microalgae algae mud for extracting in the microalgae grease that contains
Theoretical weight.
Embodiment 2
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 75% water content is added in flash extracter, is extracted in the case where 60V extracts voltage
2.5min obtains the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.7 mixing, sufficiently
It mixes, stands 1h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:2:1 DL- is thin
The mixture of lotus alcohol, pyruvic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 1.0T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 97.85%.
Embodiment 3
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 40% water content is added in flash extracter, is extracted in the case where 70V extracts voltage
1.5min obtains the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:1 mixing, it is sufficiently mixed
It is even, 1.5h is stood, magnetic hydrophobic eutectic solvent phase is separated, magnetic hydrophobic eutectic solvent is that molar ratio is 1:2:1 DL- is thin
The mixture of lotus alcohol, lactic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 1.0T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 98.90%.
Embodiment 4
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 60% water content is added in flash extracter, extracts 2min in the case where 50V extracts voltage,
Obtain the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.6 mixing, sufficiently
It mixes, stands 1.2h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:1:1 DL-
The mixture of menthol, levulic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 0.7T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 97.34%.
Embodiment 5
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 55% water content is added in flash extracter, extracts 2min in the case where 55V extracts voltage,
Obtain the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.6 mixing, sufficiently
It mixes, stands 0.9h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:1:1 DL-
The mixture of menthol, acetic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 0.9T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 97.88%.
Embodiment 6
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 65% water content is added in flash extracter, is extracted in the case where 65V extracts voltage
1.7min obtains the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.8 mixing, sufficiently
It mixes, stands 1.2h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:2:1 DL-
The mixture of menthol, lactic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 0.7T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 98.03%.
Embodiment 7
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 55% water content is added in flash extracter, is extracted in the case where 65V extracts voltage
1.5min obtains the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.8 mixing, sufficiently
It mixes, stands 0.8h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:1:1 DL-
The mixture of menthol, lactic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 0.8T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 94.18%.
Embodiment 8
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 65% water content is added in flash extracter, is extracted in the case where 55V extracts voltage
1.5min obtains the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.9 mixing, sufficiently
It mixes, stands 1h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:2:1 DL- is thin
The mixture of lotus alcohol, acetic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 0.6T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 95.75%.
Embodiment 9
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 45% water content is added in flash extracter, is extracted in the case where 50V extracts voltage
2.2min obtains the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.7 mixing, sufficiently
It mixes, stands 1h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:1:1 DL- is thin
The mixture of lotus alcohol, pyruvic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 0.9T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 93.67%.
Embodiment 10
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 85% water content is added in flash extracter, extracts 3min in the case where 75V extracts voltage,
Obtain the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.3 mixing, sufficiently
It mixes, stands 2h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:2:1 DL- is thin
The mixture of lotus alcohol, lactic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 0.5T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 92.17%.
Embodiment 11
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 30% water content is added in flash extracter, is extracted in the case where 40V extracts voltage
0.7min obtains the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.5 mixing, sufficiently
It mixes, stands 0.5h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:2:1 DL-
The mixture of menthol, lactic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 1.2T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 95.34%.
Embodiment 12
A kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, this method include following step
Suddenly：
1) microalgae algae mud with 20% water content is added in flash extracter, is extracted in the case where 80V extracts voltage
0.5min obtains the algae slurries containing microalgae grease；
It 2) is 1 according to volume ratio with magnetic hydrophobic eutectic solvent by the algae slurries containing microalgae grease:0.4 mixing, sufficiently
It mixes, stands 1.7h, separate magnetic hydrophobic eutectic solvent phase, magnetic hydrophobic eutectic solvent is that molar ratio is 1:1:1 DL-
The mixture of menthol, lactic acid and anhydrous ferric trichloride；
3) the magnetic bar magnet for being 1.5T is added in the magnetic hydrophobic eutectic solvent phase separated, recycles magnetic hydrophobic eutectic
Solvent obtains microalgae grease, recovery rate 92.23%.
Parameter is investigated
Step 1) of the present invention to each parameter in 3) is investigated respectively, is measured under different parameters, microalgae grease mentions
Rate is taken, carrying out investigation to a certain parameter the results are shown in Table 1, and unchanged each parameter is the same as embodiment 1.
Each parameter investigates result in 1 step S1 of table
As can be seen from the table, water content, extraction voltage, extraction time etc. have the recovery rate of microalgae grease significant
It influences, when water content is not within the scope of 20%-80%, and the volume ratio of algae slurries and magnetic hydrophobic eutectic solvent is not 1:
When in the range of 0.3-1, separated since magnetic hydrophobic eutectic solvent and algae slurries are bad or the big influence microalgae grease of water content
Content in magnetic hydrophobic eutectic solvent, and then cause the recovery rate of microalgae grease low；By above data as can be seen that
The selection of magnetic hydrophobic eutectic solvent has significant impact to the recovery rate of microalgae grease；The voltage of homogenate extraction and extraction
Time, which all has recovery rate, to be significantly affected, when extract that voltage is low or extraction time in short-term, extract it is insufficient cause recovery rate low,
When extraction overtension or the extraction time too long lubricant component that can be broken in meeting microalgae bath mud, and then influence recovery rate；When standing
Between also have an impact to recovery rate, upon standing between in short-term, microalgae grease not completely into magnetic hydrophobic eutectic solvent,
Too long between upon standing, the low eutectic solvent of magnetic hydrophobic separates insufficient with microalgae grease, and then can all reduce recovery rate；When
The magnetism of bar magnet is too high or too low all to be had an impact the recovery rate of microalgae grease.
Claims (9)
1. a kind of method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction prepares microalgae grease, which is characterized in that the side
Method includes the following steps：
1) it will be added in flash extracter with the microalgae algae mud of certain water content, a timing extracted in the case where centainly extracting voltage
Between, obtain the algae slurries containing microalgae grease；
2) the algae slurries containing microalgae grease prepared by step 1) are mixed well with magnetic hydrophobic eutectic solvent, is stood, separation
Magnetic hydrophobic eutectic solvent phase；
3) bar magnet is added in the magnetic hydrophobic eutectic solvent phase separated to step 2), recycles magnetic hydrophobic eutectic solvent, obtains
To microalgae grease.
2. the method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction as described in claim 1 prepares microalgae grease, special
Sign is that magnetic hydrophobic eutectic solvent described in step 2) is that molar ratio is 1:1-2:1 DL- menthol, organic acid and nothing
The mixture of aqueous ferric chloride.
3. the method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction as claimed in claim 2 prepares microalgae grease, special
Sign is that the organic acid is selected from one of acetic acid, pyruvic acid, levulic acid or lactic acid or a variety of；Preferably, the magnetism
Hydrophobic eutectic solvent is molar ratio 1:1:1 DL- menthol, the mixture of acetic acid and anhydrous ferric trichloride or molar ratio are
1:2:1 DL- menthol, the mixture of pyruvic acid and anhydrous ferric trichloride or molar ratio are 1:1:1 DL- menthol, acetyl
The mixture or molar ratio of propionic acid and anhydrous ferric trichloride are 1:2:1 DL- menthol, lactic acid and anhydrous ferric trichloride it is mixed
Close object.
4. the method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction as described in claim 1 prepares microalgae grease, special
Sign is that the water content of microalgae bath mud is 20%-85% in step 1), it is preferable that water content 40%-75%.
5. the method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction as described in claim 1 prepares microalgae grease, special
Sign is that it is 40-80V that voltage is extracted in step 1), it is preferable that extraction voltage is 50-70V.
6. the method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction as described in claim 1 prepares microalgae grease, special
Sign is that extraction time is 0.5-3min in step 1), it is preferable that extraction time 1-2.5min.
7. the method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction as described in claim 1 prepares microalgae grease, special
Sign is that the volume ratio of the algae slurries in step 2) containing microalgae grease and magnetic hydrophobic eutectic solvent is 1:0.3-1, preferably
Ground, volume ratio 1:0.5-1:1.
8. the method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction as described in claim 1 prepares microalgae grease, special
Sign is, time of repose 0.5-2h, it is preferable that time of repose 0.7-1.5h.
9. the method that magnetic hydrophobic eutectic solvent auxiliary homogenate extraction as described in claim 1 prepares microalgae grease, special
Sign is that the magnetism of bar magnet is 0.5-1.5T in step 3).
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| CN110156655A (en) * | 2019-06-04 | 2019-08-23 | 河西学院 | The method of ultrasonic wave added hydrophilic-hydrophobic two-phase system preparation microalgae beta carotene |
| CN114085141A (en) * | 2021-10-28 | 2022-02-25 | 雅邦绿色过程与新材料研究院南京有限公司 | Method for extracting and separating 3-hydroxypropionic acid by using hydrophobic eutectic solvent |
| CN114164041A (en) * | 2021-12-29 | 2022-03-11 | 润科生物工程（福建）有限公司 | Method for extracting oil contained in mortierella alpina |
| CN114292527A (en) * | 2021-12-28 | 2022-04-08 | 中国科学院兰州化学物理研究所 | Method for extracting maize yellow component from maize |
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| WEI JIANG，LEIDONG，WEI LIU，TAO GUO，等: "Biodegradable choline-like deep eutectic solvents for extractive desulfurization of fuel", 《CHEMICAL ENGINEERING AND PROCESSING - PROCESS INTENSIFICATION 》 * |
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| CN114085141A (en) * | 2021-10-28 | 2022-02-25 | 雅邦绿色过程与新材料研究院南京有限公司 | Method for extracting and separating 3-hydroxypropionic acid by using hydrophobic eutectic solvent |
| CN114085141B (en) * | 2021-10-28 | 2024-02-20 | 中国科学院过程工程研究所 | Method for extracting and separating 3-hydroxy propionic acid by using hydrophobic eutectic solvent |
| CN114292527A (en) * | 2021-12-28 | 2022-04-08 | 中国科学院兰州化学物理研究所 | Method for extracting maize yellow component from maize |
| CN114292527B (en) * | 2021-12-28 | 2022-10-04 | 中国科学院兰州化学物理研究所 | Method for extracting maize yellow component from maize |
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