CN114225997B

CN114225997B - Detection device for gene detection

Info

Publication number: CN114225997B
Application number: CN202111653511.XA
Authority: CN
Inventors: 张金银; 巩赞博; 巩赞斌; 巩赞华; 黄咏华; 彭劼
Original assignee: Huachenyang Shenzhen Technology Co ltd
Current assignee: Huachenyang Shenzhen Technology Co ltd
Priority date: 2021-12-31
Filing date: 2021-12-31
Publication date: 2022-11-22
Anticipated expiration: 2041-12-31
Also published as: CN114225997A

Abstract

The invention relates to a detection device for gene detection, belongs to the technical field of gene detection, and solves the problems that in the existing gene sample storage technology, high-power refrigeration equipment is needed to maintain the low-temperature environment of a storage place, the energy consumption is large, and the storage place is communicated with the outside every time the storage place is opened, so that the energy consumption is further increased, and dust and bacteria in the outside air pollute the storage environment of the storage place; this scheme includes the shell body, is provided with the stake body in the shell body, be provided with the storage body on the stake body, semiconductor cooler, the vacuum pump, input device, sterilizing equipment, output device, the vacuum pump makes the internal portion of storage maintain the vacuum state, semiconductor cooler makes the internal portion of storage maintain the low temperature state, store the internal bearing device that is provided with, input device pulls the test tube and gets into the storage body, bearing device supports the test tube, output device pulls the test tube output, sterilizing equipment disinfects to the test tube.

Description

Detection device for gene detection

Technical Field

The invention relates to the technical field of gene detection, in particular to a detection device for gene detection.

Background

The gene sample and the detection reagent required by the gene detection need to be stored in a low-temperature environment, the existing gene detection device has an unsatisfactory effect on the construction of the storage environment of the storage place of the gene sample and the detection reagent, and the following problems exist: 1. refrigeration equipment with high power is required to maintain the low-temperature environment of a storage place, and the energy consumption is high; 2. when the storage place is opened each time, a new test tube containing a gene test sample is placed in the storage place or a test tube containing a test sample to be detected is taken out of the storage place, the storage environment in the storage place is communicated with the outside, on one hand, the energy consumption is further increased, on the other hand, when outside air enters the storage place, dust and bacteria in the outside air pollute the storage environment in the storage place; 3. the test tube is generally held by a worker and placed in a storage place, and bacterial dust is possibly adhered to the outer surface of the test tube, so that the storage environment of the storage place is polluted; therefore, the invention provides a detection device for gene detection.

Disclosure of Invention

In order to solve the problems mentioned in the background above, the present invention provides a detection apparatus for gene detection.

In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows.

The utility model provides a detection apparatus for be used for gene detection, which comprises an outer shell, be provided with the stake body in the shell body, be provided with inside hollow storage body on the stake body, still be provided with semiconductor cooler on the stake body, the vacuum pump, input device, sterilizing equipment, output device, the inside of storage body is provided with supporting device, the vacuum pump is used for the inside air of suction storage body and makes the internal portion of storage maintain vacuum state, semiconductor cooler is used for making the internal portion of storage body maintain at low temperature state, input device is arranged in pulling the test tube entering storage body, supporting device is used for carrying out the bearing to the test tube, output device is used for pulling the internal test tube output of storage, sterilizing equipment is used for carrying out germicidal treatment to the test tube at the in-process that input device pulled the test tube.

Further, the outside of shell body is provided with puts in platform and workstation, puts in the bench and is provided with and puts in the mouth, is provided with on the workstation and gets to take the mouth, and the outside of shell body still is provided with the scanning end that is close to and puts in the platform.

Furthermore, the supporting device comprises a chain wheel and chain component arranged in the storage body, chain wheels in the chain wheel and chain component are vertically arranged, the input end of the chain wheel and chain component is in power connection with a driving motor, and a chain in the chain wheel and chain component is vertically provided with a supporting cylinder shell;

the bearing cartridge shell is vertical arranges and upper and lower both ends open-ended cylindrical shell structure, and the bearing cartridge shell divide into two sections cylinders that the diameter is unequal and be formed with the bearing shaft shoulder between two sections cylinders along self axial, and the bearing shaft shoulder cooperates and is used for supporting the test tube with the external step that sets up on the test tube, and the bearing cartridge shell is provided with the multiunit along the extending direction array of chain.

Furthermore, the input device and the output device both comprise a conveying mechanism and a traction mechanism;

the conveying mechanism comprises an installation support arranged on the upper end face of the storage body, an upper end cover and a lower end cover which are vertical in the axial direction and coaxially arranged are arranged on the installation support, a rotating body is rotatably arranged between the upper end cover and the lower end cover, and the rotating body is in sealing fit with the upper end cover and the lower end cover;

the up end of rotator coaxial extension has the rotation axis and offers on the upper end cover to be used for dodging the hole of dodging of rotation axis, and the input power of rotation axis is connected with the rotating electrical machines.

Furthermore, the end face of the rotating body is provided with conveying holes penetrating through the axial direction, and two groups of conveying holes are arranged in an array along the circumferential direction of the rotating body and are respectively a conveying hole a and a conveying hole b;

the end face of the upper end cover is provided with an upper through hole penetrating through the axial direction, two groups of upper through holes are arranged and respectively are an upper through hole a and an upper through hole b, in an initial state, the upper through hole a is communicated with the conveying hole a, an orifice of the upper through hole a vertically extends upwards to form a guide pipe, and the upper through hole b is communicated with the conveying hole b;

a guide pipe in the input device is communicated with the putting-in port, and a guide pipe in the output device is communicated with the taking-out port;

the end face of the lower end cover is provided with lower through holes which penetrate through the axial direction, the lower through holes are provided with two groups of lower through holes a and lower through holes b, the lower through holes a are communicated with the conveying holes a in the initial state, the lower through holes b are communicated with the conveying holes b, the lower through holes b are in sealing fit with the upper surface of the storage body, and the communication holes used for communicating the lower through holes b with the bearing cylinder shell are formed in the upper surface of the storage body.

Furthermore, the traction mechanism comprises a traction motor and a screw rod which are arranged on the mounting bracket, the output end of the traction motor is in power connection with the input end of the screw rod, the traction bracket is arranged on the external thread of the screw rod, the surface of the storage body is provided with a guide hole, and the traction bracket and the guide hole form sealed sliding guide fit;

the traction support is vertically provided with a traction rod, the top end of the traction rod is coaxially provided with a traction block, and in an initial state, the traction block and the lower through hole b are coaxially arranged, and the upper end face of the traction block is flush with the lower end face of the bearing cylinder shell.

Further, a sealing cover shell is arranged at an upper orifice of an upper through hole b in the conveying mechanism in the input device;

the input device further comprises a pressing component, the pressing component comprises a pressing block, the pressing block is located in the conveying hole b in an initial state, the distance between the traction block and the pressing block is matched with the axial length of the test tube, a pressing rod extends coaxially from the upper end face of the pressing block, and the pressing rod penetrates through the sealing housing and is connected with the traction support.

Furthermore, the inside slidable mounting of lower through-hole a among the conveying mechanism among the input device has the buffering stopper, lower drill way department is provided with the buffering housing, and the coaxial extension in bottom of buffering stopper has the guide arm, constitutes the ascending slip guide cooperation of vertical side between guide arm and the buffering housing, and the outside cover of guide arm is equipped with the spring that is located between buffering stopper and the buffering housing chamber bottom, and the vertical rebound of buffering stopper is ordered about to the compression elasticity of spring.

Furthermore, the end face of an upper end cover in the conveying mechanism in the input device is provided with an exhaust hole penetrating through the axial direction, the exhaust hole is positioned on the rotating track of the upper through hole, and an exhaust nozzle is arranged at the upper orifice of the exhaust hole;

an axial air inlet hole is formed in the end face of a lower end cover in a conveying mechanism in the input device, the air inlet hole and the exhaust hole are coaxially arranged, and an air inlet nozzle is arranged at the lower orifice of the air inlet hole;

the sterilizing device comprises an atomizing mechanism arranged on the bracket body, the atomizing mechanism comprises an atomizing tank vertically arranged on the bracket body, the bottom end of the atomizing tank is provided with an opening and an ultrasonic atomizer, and the top of the atomizing tank is provided with an opening and a tank cover;

the tank cover is of a table-shaped structure, the horizontal cross-sectional area of the tank cover is gradually reduced from bottom to top along the vertical direction, a connecting nozzle c is arranged at the top of the tank cover, an air inlet pipe for communicating the connecting nozzle c and the air inlet nozzle is arranged between the connecting nozzle c and the air inlet nozzle, and the free end of the air outlet nozzle is connected with an air outlet pipe;

the side of atomizing jar is provided with connector b, and connector b's free end is connected with the air pump, and the side of atomizing jar still level is provided with the lug, and the lug is close to the ultrasonic atomization ware, the inside spread groove that is provided with the atomizing jar intercommunication of lug, the up end of lug be provided with the connector a of spread groove intercommunication, the free end of connector a is connected with the connecting pipe, the other end of connecting pipe stretches out the shell body and detachable the installation alcohol jar, the height that highly is higher than the atomizing jar of alcohol jar.

Further, the output device further comprises a linear screw rod stepping motor, the linear screw rod stepping motor is installed at the bottom of the lower end cover in the output device, the linear screw rod stepping motor and a lower through hole a in the output device are coaxially arranged, and a sealing plug is arranged in an upper through hole b in a conveying mechanism in the output device.

Compared with the prior art, the invention has the beneficial effects that:

1. the test tube in the scheme is stored in a vacuum low-temperature environment, on one hand, the vacuum low-temperature environment is beneficial to storing the sample in the test tube, on the other hand, heat transfer is slow in a vacuum state, and refrigeration energy consumption is reduced;

no matter in the test tube input storage stage or the test tube output detection stage of the scheme, the storage environment in the storage body is not directly communicated with the outside, so that the energy consumption is further reduced;

in summary, the problem 1 mentioned in the background art is solved;

2. in the test tube input and storage stage, the test tube in the conveying hole is sterilized and disinfected by the alcohol aerosol provided by the sterilizing device, after sterilization is finished, the gap between the test tube and the conveying hole is filled with the alcohol aerosol, and the conveying hole a is in a sterilization environment, so that the storage environment in the storage body is not polluted by outside air in the test tube input and storage stage;

in the test tube output detection stage, the test tube is jacked into one conveying hole, then pulled into the other conveying hole and finally jacked for output, namely, the storage environment in the storage body is not directly communicated with the external environment in the test tube output process;

in conclusion, problems 2 and 3 mentioned in the background art are solved;

3. in the scheme, the storage body is refrigerated through the semiconductor refrigerator, the semiconductor refrigerator is attached to the outer surface of the storage body, and does not need to be in contact with the inside of the storage body, namely, the vacuum environment in the storage body is not interfered;

4. in the sterilization process of the scheme, the alcohol aerosol is used for sterilizing and disinfecting the test tube from bottom to top, so that the alcohol aerosol can be more fully contacted with the test tube, the sterilization and disinfection effects are better, if the alcohol aerosol is from top to bottom, the alcohol aerosol is contacted with the test tube cover, and then the alcohol aerosol can be diffused between the test tube body and the wall of the conveying hole and is insufficiently contacted with the test tube body, and the sterilization and disinfection effects are poorer because the diameter of the test tube cover is larger than that of the test tube body;

5. in the test tube storage process, in the process of putting the test tube into the conveying hole, the test tube is buffered through the matching of the buffer plug and the spring, and in the process of moving the test tube into the bearing cylinder shell, the test tube is matched with the lower pressing block through the traction block without collision;

to sum up, in the test tube save process, the test tube all receives the protection, can not take place the damaged problem of collision.

Drawings

FIG. 1 is a first schematic structural diagram of the present invention;

FIG. 2 is a second structural diagram of the present invention;

FIG. 3 is a first schematic view of the internal structure of the outer housing of the present invention;

FIG. 4 is a second schematic view of the internal structure of the outer casing of the present invention;

FIG. 5 is a schematic view of the internal structure of the storage body of the present invention;

FIG. 6 is a bottom view of the storage body of the present invention;

FIG. 7 is a schematic view of the supporting apparatus of the present invention;

FIG. 8 is a cross-sectional view of the support cartridge of the present invention;

FIG. 9 is a schematic structural diagram of an input device according to the present invention;

FIG. 10 is a schematic structural diagram of an output device according to the present invention;

FIG. 11 is a schematic structural view of a conveying mechanism of the present invention;

FIG. 12 is an exploded view of the delivery mechanism in the input device of the present invention;

FIG. 13 is a first schematic view of the drawing mechanism and the pressing member of the present invention;

FIG. 14 is a second schematic structural view of the pulling mechanism and the pressing member of the present invention;

FIG. 15 is a schematic structural view of a sterilization device according to the present invention;

FIG. 16 is a cross-sectional view of the atomizing mechanism of the present invention;

FIG. 17 is a schematic view of the structure of the test tube of the present invention.

The reference numbers in the drawings are:

100. an outer housing; 101. a scanning end; 102. a throwing port; 103. a work table; 104. taking the mouth; 105. a stent body; 106. a storage body; 107. a semiconductor refrigerator; 108. a vacuum pump;

200. a supporting device; 201. a sprocket chain member; 202. a drive motor; 203. a bearing cylinder shell; 204. supporting the shaft shoulder;

300. an input device; 301. an exhaust hole; 302. an exhaust nozzle; 303. sealing the housing; 304. an air intake; 305. an air inlet nozzle; 306. a buffer housing; 307. a buffer plug; 308. a spring;

310. a pressing member; 311. a lower pressure lever; 312. pressing the block;

400. a sterilizing device; 401. an alcohol tank; 402. a connecting pipe; 403. an atomization mechanism; 4031. an atomizing tank; 4032. a lug; 4033. connecting grooves; 4034. a connecting nozzle a; 4035. a connecting nozzle b; 4036. an ultrasonic atomizer; 4037. a can cover; 4038. a connecting nozzle c; 404. an air inlet pipe; 405. an air pump; 406. an exhaust pipe;

500. an output device; 510. a linear screw rod stepping motor;

600. a conveying mechanism; 610. a rotating body; 611. a rotating shaft; 612. a delivery orifice; 620. an upper end cover; 621. an upper through hole; 630. a lower end cover; 631. a lower through hole; 640. a rotating electric machine; 650. a guide tube;

700. a traction mechanism; 701. a traction motor; 702. a screw rod; 703. a traction support; 704. a draw bar; 705. and a traction block.

Detailed Description

To further illustrate the technical means and effects of the present invention adopted to achieve the predetermined objects, the following detailed description of the embodiments, structures, features and effects according to the present invention will be made with reference to the accompanying drawings and preferred embodiments.

As shown in FIG. 17, the test tube that is equipped with gene sample generally comprises test tube body and the test tube lid of installing at the test tube body open end, and the outside extension of test tube body has external step, and when the test tube lid was installed on test tube body, external step and test tube lid contacted.

With the continuous development of science and technology, two-dimensional codes or bar codes and the like are generally adhered to the existing test tubes containing gene samples, and the information of sample owners in the test tubes is obtained by scanning the two-dimensional codes or the bar codes.

As shown in fig. 1-6, a detection device for gene detection includes an outer casing 100, a feeding table and a workbench 103 are disposed outside the outer casing 100, a feeding port 102 is disposed on the feeding table, a taking port 104 is disposed on the workbench 103, and a scanning end 101 close to the feeding table is disposed outside the outer casing 100.

A support body 105 is arranged in the outer shell 100, a storage body 106, a semiconductor refrigerator 107 and a vacuum pump 108 are arranged on the support body 105, wherein the storage body 106 is hollow inside, the vacuum pump 108 is used for sucking air inside the storage body 106 and maintaining the inside of the storage body 106 in a vacuum state, the semiconductor refrigerator 107 is used for maintaining the inside of the storage body 106 in a low-temperature state, and the semiconductor refrigerator 107 and the vacuum pump 108 can be realized by the prior art and are not described in detail.

The inside of the storage body 106 is provided with supporting device 200, be provided with input device 300 on the support body 105, sterilizing equipment 400, output device 500, wherein, input device 300 is arranged in receiving the test tube of putting through putting into mouthful 102 input and pulls the test tube and get into the storage body 106, supporting device 200 is arranged in receiving the test tube that input device 300 input to the storage body 106 and carrying it, output device 500 is used for pulling the test tube in supporting device 200 and exports through getting by mouthful 104, sterilizing equipment 400 is used for carrying out germicidal treatment to the test tube at the in-process that input device 300 pulls the test tube.

The working principle of the scheme is divided into two stages: the input of test tube is preserved the stage, and the output of test tube detects the stage, and is specific:

an input storage stage: firstly, a worker scans a bar code on a test tube through a scanning end 101, reads and stores owner information of a sample in the test tube through a computer arranged on an outer shell 100, and simultaneously, a preset program on the computer is started to enable a supporting device 200 to start to operate, the supporting device 200 operates to enable a supporting cylinder shell 203 which is empty in the supporting device to move to a position right below a discharging end of an input device 300, and the computer records the serial number of the supporting cylinder shell 203;

then, the staff inserts the test tube to putting in mouth 102, and the test tube drops to the feed end of input device 300 under the action of gravity, is followed by input device 300 traction and is removed to the bearing cartridge case 203 that is located directly below the output end of input device 300, and this in-process, sterilizing equipment 400 carries out contactless sterilization to the test tube.

In the input saving stage: 1. the test tube is stored in a vacuum low-temperature environment, on one hand, the vacuum low-temperature environment is beneficial to storing a sample in the test tube, and on the other hand, heat is transferred slowly in a vacuum state, so that the refrigeration energy consumption is reduced; 2. semiconductor cooler 107 is attached to the outer surface of storage body 106 without contacting the interior of storage body 106, i.e., without disturbing the vacuum environment within storage body 106; 3. the test tube is at the in-process that removes to the storage body 106, and sterilizing equipment 400 carries out contactless sterilization to the test tube, disinfects the back promptly to the test tube, and the test tube does not take place the contact with external environment or staff, directly is drawn to the storage body 106 in, on the one hand, prevents that the surface of test tube from receiving secondary pollution, and on the other hand, the test tube is when entering into the storage body 106, can not bring the bacterium dust in the outside air into the storage body 106 in, makes the save environment in the storage body 106 good.

And (3) an output detection stage: firstly, a worker operates the computer, inputs the serial number of the test tube to be taken, and a preset program on the computer is started, so that the bearing cylinder shell 203 storing the test tube with the serial number in the bearing device 200 moves to the position right below the feeding end of the output device 500;

the output device 500 then operates to pull the numbered test tubes through the pick-up ports 104 onto the table 103.

As shown in fig. 5 and 7-8, the supporting device 200 includes a sprocket chain member 201 installed in the storage body 106 and a sprocket in the sprocket chain member 201 is vertically disposed, the sprocket chain member 201 can be realized for the prior art, and is not described again, the input end power of the sprocket chain member 201 is connected with a driving motor 202, specifically, the driving motor 202 is installed on the outer surface of the storage body 106, and the output end extends into the storage body 106 and is connected with the input end power of the sprocket chain member 201.

The vertical bearing cartridge case 203 of installing on the chain of sprocket chain part 201, bearing cartridge case 203 are vertical arranges and upper and lower both ends open-ended cylindrical shell structure, and bearing cartridge case 203 is divided into two sections cylinders that the diameter is unequal and is formed with bearing shaft shoulder 204 between two sections cylinders along self axial, and bearing shaft shoulder 204 cooperates with the external step that sets up on the test tube and is used for supporting the test tube.

The retainer cylinder housings 203 are arranged in a plurality of sets in an array along the extending direction of the chain, and each set of retainer cylinder housings 203 has a corresponding number in the computer.

The operation of the driving motor 202 drives the chain in the sprocket chain member 201 to pull the supporting cylinder 203 to move, so that the empty supporting cylinder 203 moves to the position right below the discharging end of the input device 300, or the supporting cylinder 203 for storing test tubes moves to the position right below the feeding end of the output device 500.

As shown in fig. 9-10, the input device 300 and the output device 500 each include a conveying mechanism 600 and a pulling mechanism 700.

Specifically, as shown in fig. 9 to 12, the conveying mechanism 600 includes a mounting bracket mounted on the upper end surface of the storage body 106, an upper end cap 620 and a lower end cap 630 which are axially vertical and coaxially arranged are mounted on the mounting bracket, the rotary body 610 is rotatably mounted between the upper end cap 620 and the lower end cap 630, and the rotary body 610 and the upper end cap 620 and the rotary body 610 and the lower end cap 630 form a sealing fit.

The upper end surface of the rotating body 610 coaxially extends to form a rotating shaft 611, an avoiding hole for avoiding the rotating shaft 611 is formed in the upper end cover 620, the input end of the rotating shaft 611 is in power connection with the rotating motor 640 through a belt transmission structure, and the rotating motor 640 drives the rotating shaft 611 to rotate when operating, so that the rotating body 610 rotates.

The end face of the rotating body 610 is provided with two groups of conveying holes 612 penetrating through the axial direction, the two groups of conveying holes 612 are arranged in an array along the circumferential direction of the rotating body 610 and respectively form a conveying hole a and a conveying hole b, namely, an included angle between the two groups of conveying holes 612 is one hundred eighty degrees.

The end face of the upper end cover 620 is provided with an upper through hole 621 penetrating through the axial direction, the upper through hole 621 is provided with two groups of upper through holes a and upper through holes b, wherein in an initial state, the upper through hole a is communicated with the delivery hole a, a guide pipe 650 vertically extends upwards from a hole opening of the upper through hole a, and the upper through hole b is communicated with the delivery hole b.

The guide tube 650 in the input device 300 communicates with the input port 102, and the guide tube 650 in the output device 500 communicates with the pickup port 104.

The end face of the lower end cap 630 is provided with lower through holes 631 penetrating the axial direction, and the lower through holes 631 are provided with two sets of lower through holes a and lower through holes b, wherein in an initial state, the lower through holes a are communicated with the conveying holes a, the lower through holes b are communicated with the conveying holes b, the lower through holes b are in sealing fit with the upper surface of the storage body 106, and the upper surface of the storage body 106 is provided with communication holes for communicating the lower through holes b with the bearing cartridge housing 203.

As shown in fig. 9-10 and 13-14, the traction mechanism 700 includes a traction motor 701 and a screw rod 702 mounted on the mounting bracket, an output end of the traction motor 701 is in power connection with an input end of the screw rod 702, and the traction motor 701 operates to drive the screw rod 702 to rotate.

The external thread of the screw rod 702 is provided with a traction bracket 703, the surface of the storage body 106 is provided with a guide hole, and the traction bracket 703 and the guide hole form a sealed sliding guide fit.

A traction rod 704 is vertically arranged on the traction bracket 703, a traction block 705 is coaxially arranged at the top end of the traction rod 704, and in an initial state, the traction block 705 is coaxially arranged with the lower through hole b, and the upper end surface of the traction block 705 is flush with the lower end surface of the bearing cylinder shell 203.

As shown in fig. 9, 12, and 14, a sealing cover 303 is mounted at an upper orifice of an upper through hole b in the conveying mechanism 600 in the input device 300.

The input device 300 further comprises a pressing member 310, the pressing member 310 comprises a pressing block 312, and in the initial state, the pressing block 312 is located in the conveying hole b, and the distance between the drawing block 705 and the pressing block 312 is matched with the axial length of the test tube.

A lower pressure rod 311 coaxially extends from the upper end surface of the lower pressure block 312, and the lower pressure rod 311 penetrates through the sealing cover 303 and is connected with the traction bracket 703.

The end surface of the upper end cover 620 in the conveying mechanism 600 in the input device 300 is provided with an exhaust hole 301 penetrating through the axial direction, the exhaust hole 301 is positioned on the rotation track of the upper through hole 621, and an exhaust nozzle 302 is arranged at the upper orifice of the exhaust hole 301.

The end surface of the lower end cover 630 of the conveying mechanism 600 in the input device 300 is provided with an air inlet hole 304 penetrating through the axial direction, the air inlet hole 304 and the air outlet hole 301 are coaxially arranged, and the lower opening of the air inlet hole 304 is provided with an air inlet nozzle 305.

The working process of the input device 300 is specifically as follows:

firstly, the traction motor 701 operates to drive the screw rod 702 to rotate, so that the lower pressing block 312 is positioned in the upper through hole b, and the traction block 705 is positioned in the lower through hole b;

then, the test tube is put into the conveying hole a through the putting port 102, the guide tube 650 and the upper through hole a in sequence;

then, the rotating motor 640 operates to draw the rotating body 610 to rotate, so that the conveying hole a is located between the air outlet hole 301 and the air inlet hole 304, the medical alcohol aerosol provided by the sterilization device 400 enters the conveying hole a through the air inlet hole 304 and sterilizes and disinfects the test tube, the sterilized medical alcohol aerosol is discharged through the air outlet hole 301, and after sterilization is finished, the alcohol aerosol exists between a gap between the conveying hole a and the test tube, and the conveying hole a is in a sterilization environment;

then, the rotary motor 640 operates the traction rotation body 610 to rotate, so that the conveying hole a is positioned at the upper through hole b and the lower through hole b;

then, the traction motor 701 operates to drive the screw 702 to rotate, so that the lower pressing block 312 and the traction block 705 vertically move downwards, the lower pressing block 312 and the traction block 705 vertically move downwards and pull the test tube in the conveying hole a to synchronously move downwards, finally, the test tube is pulled into an empty supporting cylinder shell 203, and the supporting cylinder shell 203 supports and supports the test tube through the matching of the supporting shaft shoulder 204 and the external step.

At this point, the input and storage of the test tube is finished, and at this point, the position of the conveying hole a and the position of the conveying hole b are exchanged, and the input and storage process of the next test tube is consistent with the above.

In a preferred embodiment, as shown in fig. 12, a buffer plug 307 is slidably installed inside a lower through hole a in a conveying mechanism 600 in an input device 300, a buffer housing 306 is arranged at a lower opening, a guide rod coaxially extends from the bottom of the buffer plug 307, a sliding guide fit in the vertical direction is formed between the guide rod and the buffer housing 306, a spring 308 located between the buffer plug 307 and the bottom of the buffer housing 306 is sleeved outside the guide rod, and the compression elastic force of the spring 308 drives the buffer plug 307 to move vertically upwards; the test tube feeding device has the significance that in the process of sequentially feeding the test tube into the conveying hole a through the feeding port 102, the guide tube 650 and the upper through hole a, the buffer plug 307 and the spring 308 are matched to play a buffer role.

As shown in fig. 12 and 15-16, the sterilization device 400 includes an atomizing mechanism 403 mounted on the support body 105, and the atomizing mechanism 403 is used for receiving liquid medical alcohol and converting the liquid medical alcohol into aerosol of medical alcohol.

Specifically, the atomizing mechanism 403 includes an atomizing cup 4031 vertically mounted on the support body 105, the atomizing cup 4031 being open at a bottom end thereof and mounted with the ultrasonic atomizer 4036, and open at a top end thereof and mounted with a cup cover 4037.

The tank cover 4037 is of a table-shaped structure, the horizontal cross-sectional area of the tank cover 4037 decreases progressively from bottom to top in the vertical direction, a connecting nozzle c4038 is arranged at the top of the tank cover 4037, an air inlet pipe 404 for communicating the connecting nozzle c4038 with the air inlet nozzle 305 is arranged between the connecting nozzle c4038 and the air inlet nozzle 305, and an exhaust pipe 406 is connected to the free end of the exhaust nozzle 302.

The side of the atomization tank 4031 is provided with a connecting nozzle b4035, and the free end of the connecting nozzle b4035 is connected with an air pump 405.

The side surface of the atomizing cup 4031 is further horizontally provided with a lug 4032, the lug 4032 is close to the ultrasonic atomizer 4036, the inside of the lug 4032 is provided with a connecting groove 4033 communicated with the atomizing cup 4031, and the upper end surface of the lug 4032 is provided with a connecting nozzle a4034 communicated with the connecting groove 4033.

The free end of the connecting nozzle a4034 is connected with a connecting pipe 402, the other end of the connecting pipe 402 extends out of the outer shell 100 and is detachably provided with an alcohol tank 401, and the height of the alcohol tank 401 is higher than that of the atomizing tank 4031.

The working process of the sterilization device 400 is as follows:

liquid alcohol in the alcohol tank 401 sequentially flows into the atomizing tank 4031 through the connecting pipe 402, the connecting nozzle a4034 and the connecting groove 4033, when the notch of the connecting groove 4033 is plugged by the alcohol liquid level in the atomizing tank 4031, the liquid alcohol stops flowing into the atomizing tank 4031, when the alcohol in the atomizing tank 4031 is consumed and the plugging of the notch of the connecting groove 4033 is cancelled, the liquid alcohol continues flowing into the atomizing tank 4031, namely, the liquid alcohol liquid level in the atomizing tank 4031 is kept at the critical point for plugging the notch of the connecting groove 4033, and the process can be realized in the prior art and is not described again.

When the delivery hole a is positioned between the exhaust hole 301 and the air inlet hole 304, the ultrasonic atomizer 4036 operates to atomize the liquid alcohol in the atomizing tank 4031 into gaseous alcohol aerosol, and simultaneously, the air pump 405 operates to generate air flow which pulls the alcohol aerosol to enter the delivery hole a through the tank cover 4037, the connecting nozzle c4038, the air inlet pipe 404, the air inlet nozzle 305 and the air inlet hole 304 in sequence and sterilize and disinfect the test tube, and the sterilized alcohol aerosol is discharged through the exhaust hole 301, the exhaust nozzle 302 and the exhaust pipe 406 in sequence;

after sterilization is finished, the operation of the air pump 405 and the ultrasonic nebulizer 4036 is stopped, and at this time, alcohol mist exists between the gap between the delivery hole a and the test tube, and the delivery hole a is in a sterilization environment.

As shown in fig. 10, the output device 500 further includes a linear screw stepping motor 510, the linear screw stepping motor 510 is installed at the bottom of the lower cap 630 in the output device 500, and the linear screw stepping motor 510 is coaxially arranged with the lower through-hole a in the output device 500; the linear screw stepping motor 510 is realized by the prior art and is not described in detail.

A sealing plug is provided in the upper through hole b in the conveying mechanism 600 in the output device 500.

The working principle of the invention is as follows:

firstly, the method comprises the following steps: inputting and storing test tubes;

the method comprises the following steps: the staff scans the bar code on the test tube through the scanning end 101, and reads and stores the owner information of the sample in the test tube through the computer arranged on the outer shell 100;

meanwhile, a preset program on the computer is started, so that a driving motor 202 in the supporting device 200 operates to drive a chain in a chain wheel and chain component 201 to pull a supporting cylinder shell 203 to move, the empty supporting cylinder shell 203 moves to be right below a lower through hole b in the input device 300, and the computer records the number of the supporting cylinder shell 203;

then, a traction motor 701 in a traction mechanism 700 in the input device 300 operates to drive a screw rod 702 to rotate, so that the lower pressing block 312 is positioned in the upper through hole b, and a traction block 705 penetrates through the hollow bearing cylinder shell 203 and is positioned in the lower through hole b;

step two: a worker puts the test tube into the conveying hole a through the putting opening 102, the guide pipe 650 and the upper through hole a in sequence;

step three: the rotary motor 640 of the conveying mechanism 600 in the input device 300 operates the traction rotary body 610 to rotate, so that the conveying hole a is positioned between the exhaust hole 301 and the intake hole 304;

step four: the ultrasonic atomizer 4036 in the sterilization device 400 operates to atomize liquid alcohol in the aerosol 4031 into gaseous alcohol aerosol, and meanwhile, the air pump 405 operates to generate air flow which draws the alcohol aerosol to sequentially pass through the tank cover 4037, the connecting nozzle c4038, the air inlet pipe 404, the air inlet nozzle 305 and the air inlet hole 304 to enter the conveying hole a and sterilize the test tube, and the sterilized alcohol aerosol is sequentially discharged through the exhaust hole 301, the exhaust nozzle 302 and the exhaust pipe 406;

after sterilization is finished, the air pump 405 and the ultrasonic atomizer 4036 stop running, and at this time, alcohol aerosol exists in a gap between the delivery hole a and the test tube, and the delivery hole a is in a sterilization environment;

step five: the rotary motor 640 of the conveying mechanism 600 of the input device 300 operates the traction rotating body 610 to rotate, so that the conveying hole a is positioned between the upper through hole b and the lower through hole b;

step six: the operation of a traction motor 701 in the traction mechanism 700 in the input device 300 drives a lead screw 702 to rotate, so that the lower pressing block 312 and the traction block 705 move downwards vertically, the lower pressing block 312 and the traction block 705 move downwards vertically and pull test tubes in the conveying hole a to move downwards synchronously, finally the test tubes are pulled into an empty bearing cylinder shell 203 through a lower through hole b and a communication hole formed in the upper surface of the storage body 106, and the bearing cylinder shell 203 supports and supports the test tubes through the matching of a bearing shaft shoulder 204 and an external step.

At this point, the input and storage of the test tube is finished, and at this point, the conveying hole a and the conveying hole b in the input device 300 are exchanged in position, and the input and storage process of the next test tube is the same as the above.

II, secondly, the method comprises the following steps: the output detection stage of the test tube;

the method comprises the following steps: a worker operates the computer, inputs the serial number of the test tube to be taken, and a preset program on the computer is started to enable the driving motor 202 in the supporting device 200 to operate, drive the chain in the chain wheel and chain component 201 to pull the supporting cylinder shell 203 to move, and further enable the supporting cylinder shell 203 storing the test tube with the serial number to move to be right below the lower through hole b in the output device 500;

step two: a traction motor 701 in a traction mechanism 700 in the output device 500 operates to drive a screw rod 702 to rotate, so that a traction block 705 vertically moves upwards, the traction block 705 moves upwards to jack the test tube, and the test tube moves upwards to a conveying hole b in the output device 500;

step three: the rotary motor 640 of the conveying mechanism 600 in the output device 500 operates the traction rotating body 610 to rotate, so that the conveying hole b is positioned between the upper through hole a and the lower through hole a;

step four: the linear screw rod stepping motor 510 operates to lift the test tube, and the test tube is output through the taking port 104;

step five: the traction motor 701 in the traction mechanism 700 in the output device 500 operates to drive the screw 702 to rotate, so that the traction block 705 moves downwards vertically to reset, and the traction mechanism 700 in the output device 500 resets.

At this point, the input and storage of the test tube is finished, and at this point, the conveying hole a and the conveying hole b in the output device 500 are exchanged, and the output detection process of the next test tube is the same as the above.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. A detection device for gene detection comprises an outer shell (100), wherein a support body (105) is arranged in the outer shell (100), a storage body (106) with a hollow interior is arranged on the support body (105), and the detection device is characterized in that a semiconductor refrigerator (107), a vacuum pump (108), an input device (300), a sterilization device (400) and an output device (500) are further arranged on the support body (105), a supporting device (200) is arranged inside the storage body (106), the vacuum pump (108) is used for sucking air inside the storage body (106) and maintaining the inside of the storage body (106) in a vacuum state, the semiconductor refrigerator (107) is used for maintaining the inside of the storage body (106) in a low-temperature state, the input device (300) is used for drawing a test tube into the storage body (106), the supporting device (200) is used for supporting the test tube, the output device (500) is used for drawing the test tube in the storage body (106) for outputting, and the sterilization device (400) is used for performing sterilization treatment on the test tube in the drawing process of the input device (300);

a throwing table and a workbench (103) are arranged outside the outer shell (100), a throwing port (102) is formed in the throwing table, a taking port (104) is formed in the workbench (103), and a scanning end (101) close to the throwing table is further arranged outside the outer shell (100);

the supporting device (200) comprises a chain wheel and chain component (201) arranged in the storage body (106), chain wheels in the chain wheel and chain component (201) are vertically arranged, the input end of the chain wheel and chain component (201) is in power connection with a driving motor (202), and a supporting cylinder shell (203) is vertically arranged on a chain in the chain wheel and chain component (201);

the supporting cylinder shell (203) is of a vertically-arranged cylindrical shell structure with openings at the upper end and the lower end, the supporting cylinder shell (203) is divided into two sections of cylinders with different diameters along the axial direction of the supporting cylinder shell, a supporting shaft shoulder (204) is formed between the two sections of cylinders, the supporting shaft shoulder (204) is matched with an external step arranged on a test tube and used for supporting the test tube, and multiple groups of supporting cylinder shells (203) are arranged in an array along the extending direction of a chain;

the input device (300) and the output device (500) both comprise a conveying mechanism (600) and a traction mechanism (700);

the conveying mechanism (600) comprises a mounting support mounted on the upper end face of the storage body (106), an upper end cover (620) and a lower end cover (630) which are vertical in the axial direction and coaxially arranged are mounted on the mounting support, a rotating body (610) is rotatably mounted between the upper end cover (620) and the lower end cover (630), and the rotating body (610) and the upper end cover (620) and the rotating body (610) and the lower end cover (630) are in sealing fit;

a rotating shaft (611) coaxially extends from the upper end face of the rotating body (610), an avoiding hole for avoiding the rotating shaft (611) is formed in the upper end cover (620), and the input end of the rotating shaft (611) is dynamically connected with a rotating motor (640);

the end face of the rotating body (610) is provided with conveying holes (612) penetrating through the axial direction, and two groups of conveying holes (612) are arranged in an array along the circumferential direction of the rotating body (610) and are respectively a conveying hole a and a conveying hole b;

the end face of the upper end cover (620) is provided with an upper through hole (621) which penetrates through the axial direction, two groups of upper through holes (621) are arranged and respectively are an upper through hole a and an upper through hole b, in an initial state, the upper through hole a is communicated with the conveying hole a, a guide pipe (650) vertically extends upwards from the orifice of the upper through hole a, and the upper through hole b is communicated with the conveying hole b;

a guide pipe (650) in the input device (300) is communicated with the putting port (102), and a guide pipe (650) in the output device (500) is communicated with the taking port (104);

the end face of the lower end cover (630) is provided with lower through holes (631) which penetrate through the axial direction, two groups of lower through holes (631) are arranged, namely a lower through hole a and a lower through hole b, in an initial state, the lower through hole a is communicated with the conveying hole a, the lower through hole b is communicated with the conveying hole b, the lower through hole b is in sealing fit with the upper surface of the storage body (106), and the upper surface of the storage body (106) is provided with a communicating hole for communicating the lower through hole b with the bearing cylinder shell (203);

the traction mechanism (700) comprises a traction motor (701) and a screw rod (702) which are arranged on an installation support, the output end of the traction motor (701) is in power connection with the input end of the screw rod (702), a traction support (703) is installed on the external thread of the screw rod (702), a guide hole is formed in the surface of the storage body (106), and a sealed sliding guide fit is formed between the traction support (703) and the guide hole;

a traction rod (704) is vertically arranged on the traction support (703), a traction block (705) is coaxially arranged at the top end of the traction rod (704), and in an initial state, the traction block (705) and the lower through hole b are coaxially arranged, and the upper end face of the traction block (705) is flush with the lower end face of the bearing cylinder shell (203);

a sealing cover shell (303) is installed at the upper hole of an upper through hole b in the conveying mechanism (600) in the input device (300);

the input device (300) further comprises a pressing component (310), the pressing component (310) comprises a pressing block (312), in an initial state, the pressing block (312) is located in the conveying hole b, the distance between the traction block (705) and the pressing block (312) is matched with the axial length of the test tube, a pressing rod (311) coaxially extends from the upper end face of the pressing block (312), and the pressing rod (311) penetrates through the sealing cover (303) and is connected with the traction support (703);

the buffer plug (307) is arranged in a lower through hole a of a conveying mechanism (600) in the input device (300) in a sliding mode, a buffer housing (306) is arranged at a lower hole opening, a guide rod coaxially extends from the bottom of the buffer plug (307), the guide rod and the buffer housing (306) form sliding guide fit in the vertical direction, a spring (308) located between the buffer plug (307) and the cavity bottom of the buffer housing (306) is sleeved outside the guide rod, and compression elastic force of the spring (308) drives the buffer plug (307) to move vertically upwards.

2. The detecting device for gene detection according to claim 1, wherein an end face of an upper end cover (620) in the conveying mechanism (600) in the input device (300) is provided with an exhaust hole (301) penetrating through the axial direction, the exhaust hole (301) is located on the rotating track of the upper through hole (621), and an exhaust nozzle (302) is arranged at the upper opening of the exhaust hole (301);

an axial air inlet hole (304) is formed in the end face of a lower end cover (630) in a conveying mechanism (600) in the input device (300), the air inlet hole (304) and the exhaust hole (301) are coaxially arranged, and an air inlet nozzle (305) is arranged at the lower opening of the air inlet hole (304);

the sterilization device (400) comprises an atomization mechanism (403) arranged on a support body (105), the atomization mechanism (403) comprises an atomization tank (4031) vertically arranged on the support body (105), the bottom end of the atomization tank (4031) is provided with an opening and is provided with an ultrasonic atomizer (4036), and the top end of the atomization tank is provided with an opening and is provided with a tank cover (4037);

the tank cover (4037) is of a table-shaped structure, the horizontal cross-sectional area of the tank cover (4037) is gradually reduced from bottom to top along the vertical direction, a connecting nozzle c (4038) is arranged at the top of the tank cover (4037), an air inlet pipe (404) used for communicating the connecting nozzle c (4038) with the air inlet nozzle (305) is arranged between the connecting nozzle c (4038) and the air inlet nozzle (305), and the free end of the air exhaust nozzle (302) is connected with an air exhaust pipe (406);

the side of atomizing jar (4031) is provided with connector b (4035), the free end of connector b (4035) is connected with air pump (405), the side of atomizing jar (4031) still the level is provided with lug (4032), lug (4032) are close to ultrasonic nebulizer (4036), lug (4032) inside is provided with spread groove (4033) with atomizing jar (4031) intercommunication, the up end of lug (4032) is provided with connector a (4034) with spread groove (4033) intercommunication, the free end of connector a (4034) is connected with connecting pipe (402), the other end of connecting pipe (402) stretches out shell body (100) and detachable alcohol tank (401) of installing, the height of alcohol tank (401) is higher than the height of atomizing jar (4031).

3. The apparatus for detecting gene according to claim 1, wherein the output unit (500) further comprises a linear screw stepping motor (510), the linear screw stepping motor (510) is installed at the bottom of the lower end cap (630) of the output unit (500), and the linear screw stepping motor (510) is coaxially arranged with the lower through hole a of the output unit (500), and a sealing plug is provided in the upper through hole b of the conveying mechanism (600) of the output unit (500).