CN218710438U - Cell culture dish bacterial infection experimental apparatus - Google Patents

Cell culture dish bacterial infection experimental apparatus Download PDF

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Publication number
CN218710438U
CN218710438U CN202222770003.6U CN202222770003U CN218710438U CN 218710438 U CN218710438 U CN 218710438U CN 202222770003 U CN202222770003 U CN 202222770003U CN 218710438 U CN218710438 U CN 218710438U
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China
Prior art keywords
cell culture
culture dish
connecting frame
bacterial infection
box
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CN202222770003.6U
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Chinese (zh)
Inventor
王恺皞
黄铮
杜一鹏
周敏
李培鑫
关畅
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First Affiliated Hospital of Guangzhou Medical University
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First Affiliated Hospital of Guangzhou Medical University
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Abstract

The utility model discloses a cell culture dish bacterial infection experimental apparatus relates to cell culture dish experiment field, specifically includes the device case, the opening has been seted up to one side of device case, and is provided with the sealing washer on the open-ended inside wall, the inner wall of sealing washer closely laminates and has the closing plate that runs through to the device incasement portion, the closing plate is provided with first carriage and second carriage respectively towards the upper and lower both ends of device incasement portion one side, the top of first carriage and the bottom of second carriage all are fixed with the movable block, it is connected with two first pivots to rotate through the bearing on the inner wall of device case. The utility model discloses an utilize the control box to start the motor, the motor can make the closing plate remove to the outside of device case, and then makes people can place the support network with the cell culture dish more swiftly conveniently effectively on, and operation space is bigger, and the result of use is better.

Description

Cell culture dish bacterial infection experimental apparatus
Technical Field
The utility model relates to a cell culture dish experiment field specifically is cell culture dish bacterial infection experimental apparatus.
Background
In the basic experiment of the cardiovascular medicine, the bacterial infection experiment is often needed to be carried out on a cell culture dish, the experiment has strict requirements on the infection environment, the bacterial infection experiment needs to be uniformly cultured, and the bacterial infection experiment has no mixed bacteria pollution.
The prior application number is 201611015780.2 a cell culture dish bacterial infection experimental apparatus, including installing the airtight bacterial infection cabin on the activity foot rest, it openly has sealed hatch door and hatch door hand lock to open and close and lock sealed hatch door, it can put the cell culture dish and expose in infecting experimental environment to have a plurality of under-deck trays on the cabin body back wall of bacterial infection cabin inside, bacterial infection cabin upper portion is connected the atomizing diffuser and is atomized and is diffused the liquid medicine that contains the bacterium and send out and expose and infect the experiment to the cell culture dish, bacterial infection cabin lower extreme sealing connection is to exhaust gas processor, disinfect and high-efficient filtration the waste gas that the bacterial infection cabin circulates and come, guarantee that exhaust gas discharges the safety and discharges, disinfect the disinfection with the ultraviolet disinfection lamp in the bacterial infection cabin after the experiment is accomplished. A cell culture dish bacterial infection experimental apparatus with simple structure, safety and convenience is developed, and the difficult problems of automatic operation and sealing isolation in the research work of bacterial infection of the cell culture dish are solved.
There are still drawbacks: the device utilizes the lock handle on the rotatory hatch door hand lock to open and close and lock movable door board at will, and movable door board compresses tightly the hatch adhesive tape and just can seal antetheca hatch mouth and form airtight to the bacterial infection cabin, but the cell culture dish is located the inside in bacterial infection cabin throughout among the device for its operating space receives the limitation, and the operation of not making things convenient for people influences work efficiency.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell culture dish bacterial infection experimental apparatus to solve the problem that proposes among the above-mentioned background art.
In order to realize the purpose of the utility model, the utility model adopts the following technical scheme:
the utility model provides a cell culture dish bacterial infection experimental apparatus, including the device case, the opening has been seted up to one side of device case, and is provided with the sealing washer on the open-ended inside wall, the inner wall of sealing washer closely laminates and has runs through to the inside closing plate of device case, the closing plate is provided with first connection frame and second connection frame respectively towards the upper and lower both ends of the inside one side of device case, the top of first connection frame and the bottom of second connection frame all are fixed with the movable block, rotate through the bearing on the inner wall of device case and be connected with two first pivots, one of them first pivot is located the top of first connection frame, another first pivot is located the below of second connection frame, first pivot run through the movable block and with the movable block between rotate through the screw thread and be connected, one side that the closing plate was kept away from to first connection frame and second connection frame is connected with fixed frame, fixed frame all is provided with the supporting legs of one-to-one with the opposite side of closing plate, and is provided with the supporting legs on the supporting legs, the top of supporting legs is provided with the cell culture dish, still cup joint first gear in the first pivot, and the motor control box that the motor that the fixed box was installed and the motor control box is used for the second gear box and the second gear control device is connected to the inside the pivot, the second pivot is connected to be connected to the motor is connected to the motor of second gear box, the motor control device case.
Preferably, a through hole is formed in one side, far away from the device box, of the control box, a baffle plate used for plugging the through hole is hinged to the position, close to the through hole, of the inner portion of the control box through a pin shaft, and a switch electrically connected with the motor is further arranged inside the control box.
Preferably, the sealing plate is further provided with a sealing baffle strip, and the longitudinal section of the sealing baffle strip is larger than that of the opening.
Preferably, the second connecting frame is symmetrically provided with connecting plates along the central axis thereof, the bottom of each connecting plate is provided with a chute, a support plate is arranged below the second connecting frame in the device box, and the top of the support plate is provided with a roller matched with the chute.
Preferably, the top of the support plate is symmetrically provided with electromagnetic blocks along the central axis thereof, the bottom of the connecting plate is provided with iron blocks matched with the electromagnetic blocks, and the electromagnetic blocks are electrically connected with the switch through wires.
Preferably, the top of the supporting net is uniformly provided with a placing groove, the inner side wall of the placing groove is provided with elastic balls, and the cell culture dish is located between the elastic balls.
Preferably, the bottom of the supporting net is provided with a coiled pipe, one end of the coiled pipe is connected with a corrugated pipe, and the coiled pipe is uniformly provided with atomizing nozzles along the length direction of the coiled pipe.
Compared with the prior art, the above one or more technical schemes have the following beneficial effects:
through utilizing the control box to start the motor, the motor drives the second pivot and rotates, the second pivot makes the second gear rotate, under the effect of drive belt, first gear drives first pivot and carries out synchronous rotation along with the second gear, when first pivot rotates, can make the movable block remove along first pivot length direction under the effect of screw thread, make this movable block drive first carriage, second carriage and closing plate remove, the closing plate can remove to the outside of device case, people can more swiftly make things convenient for effectively place the cell culture dish on the supporting network this moment, operating space is bigger, excellent in use effect.
Drawings
The accompanying drawings, which form a part of the specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without unduly limiting the scope of the invention.
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a schematic view of a connection structure of the sealing plate and the first connection frame of the present invention;
fig. 3 is a schematic structural view of the sealing ring of the present invention;
fig. 4 is a schematic diagram of the internal structure of the control box of the present invention;
FIG. 5 is a schematic view of the structure of the connecting plate of the present invention matching with the supporting plate;
FIG. 6 is an enlarged view of the present invention at A in FIG. 1;
fig. 7 is a schematic view of the bottom structure of the support net of the present invention;
in the figure:
1. a device box; 2. sealing plates; 3. a first connection frame; 4. a second connection frame; 5. a fixing frame; 6. supporting legs; 7. a support net; 8. a cell culture dish; 9. a first rotating shaft; 10. a moving block; 11. a first gear; 12. a fixing box; 13. a motor; 14. a second rotating shaft; 15. a second gear; 16. a transmission belt; 17. a control box; 18. an opening; 19. a seal ring; 20. sealing the barrier strip; 21. a baffle plate; 22. a switch; 23. a connecting plate; 24. a chute; 25. a support plate; 26. a roller; 27. an electromagnetic block; 28. an iron block; 29. a placement groove; 30. an elastic ball; 31. a serpentine tube; 32. a bellows; 33. an atomizing spray head.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It should be understood that the data so used may be interchanged under appropriate circumstances such that embodiments of the application described herein may be used. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In this application, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings. These terms are used primarily to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to a particular orientation or to be constructed and operated in a particular orientation.
Moreover, some of the above terms may be used to indicate other meanings besides the orientation or positional relationship, for example, the term "on" may also be used to indicate some kind of attachment or connection relationship in some cases. The specific meaning of these terms in this application will be understood by those of ordinary skill in the art as appropriate.
Furthermore, the terms "mounted," "disposed," "provided," "connected," and "sleeved" are to be construed broadly. For example, it may be a fixed connection, a removable connection, or a unitary construction; can be a mechanical connection, or an electrical connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements or components. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
The first embodiment is as follows:
referring to fig. 1-6, the present embodiment provides a bacterial infection experiment apparatus for a cell culture dish 8, which includes an apparatus box 1, wherein a pipeline penetrating to two sides of the apparatus box 1 is disposed at an inner top end of the apparatus box 1, one end of the pipeline is connected to a bacterial culture solution bottle containing a liquid inlet pump and a valve, the other end of the pipeline is connected to a compressed gas cylinder containing a valve, a middle portion of the pipeline is located inside the apparatus box 1 and is communicated with an atomizing funnel, the atomizing funnel contains a heating plate and can atomize a liquid medicine containing bacteria into fine particles to be diffused; opening 18 has been seted up to one side of device case 1, and be provided with sealing washer 19 on the inside wall of opening 18, the inner wall of sealing washer 19 closely laminates and runs through to the inside closing plate 2 of device case 1, closing plate 2 is provided with first connecting frame 3 and second connecting frame 4 respectively towards the upper and lower both ends of the inside one side of device case 1, the top of first connecting frame 3 and the bottom of second connecting frame 4 all are fixed with movable block 10, be connected with two first pivots 9 through the bearing rotation on the inner wall of device case 1, one of them first pivot 9 is located the top of first connecting frame 3, another first pivot 9 is located the below of second connecting frame 4, first pivot 9 runs through movable block 10 and is connected through the screw rotation between the movable block 10, one side that closing plate 2 was kept away from to first connecting frame 3 and second connecting frame 4 is connected with fixed frame 5, fixed frame 5 and the opposite side of closing plate 2 all are provided with the supporting legs 6 of one-to-one correspondence, and be provided with supporting legs 7 on the supporting legs 6, the top of supporting legs 7 is provided with cell culture dish 8, still cup jointed first gear 11 on the first pivot 9, one side of fixed box 1 still is provided with fixed gear 12, and be provided with the drive belt 13 that the fixed box 12 and be used for the motor device 13 is connected with the motor 14 and the second pivot 16 that the motor control device 13 is connected with the inside of being connected with the second pivot 14, it is used for the second gear 17, it extends to be connected with the motor device 13, it is connected with the second pivot 14.
When the cell culture dish device is used, the motor 13 is started by the control box 17, the motor 13 drives the second rotating shaft 14 to rotate, the second rotating shaft 14 enables the second gear 15 to rotate, the first gear 11 drives the first rotating shaft 9 to synchronously rotate along with the second gear 15 under the action of the driving belt 16, the moving block 10 can move along the length direction of the first rotating shaft 9 under the action of the screw threads when the first rotating shaft 9 rotates, so that the moving block 10 drives the first connecting frame 3, the second connecting frame 4 and the sealing plate 2 to move, and the sealing plate 2 can move to the outer part of the device box 1, at the moment, people can more quickly, conveniently and effectively place the cell culture dish 8 on the supporting net 7, the operation space is larger, and the use effect is better; after the placement is finished, the control box 17 is used again to enable the motor 13 to drive the second rotating shaft 14 to rotate reversely, so that the sealing plate 2 can return to the original position, and the outer wall of the sealing plate 2 is tightly attached to the inner wall of the sealing ring 19, so that the opening 18 can be completely blocked after the sealing plate 2 returns to the original position, the whole device box 1 is automatically sealed, the operation of personnel is not needed, and the device box is convenient and quick; when complete sealed back, open the valve in bacterial culture liquid bottle and the compressed gas bottle, make to have the compressed gas who accords with experiment requirement pressure and enter into atomizing funnel, open the feed liquor pump of atomizing funnel other end simultaneously, during bacterial culture liquid in the bacterial culture liquid bottle is carried atomizing funnel, compressed gas and bacterial culture liquid meet at atomizing funnel, the heating plate of atomizing funnel bottom remains required temperature throughout, atomizing funnel will atomize into the tiny particulate matter according to the required liquid medicine that contains the bacterium of experiment and diffuse out, and then expose infection experiment to cell culture dish 8 on the supporting network 7.
Please refer to fig. 4, a through hole is formed on one side of the control box 17 away from the device box, a baffle 21 for plugging the through hole is hinged in the control box 17 near the through hole through a pin, and a switch 22 electrically connected with the motor 13 is further arranged in the control box 17. During the use, the user promotes baffle 21 manually for baffle 21 is rotatory towards the inside of control box 17, and then makes the user touch switch 22 with the hand, is convenient for control motor 13, and when not using, baffle 21 can seal the through-hole automatically under the effect of its own gravity, avoids the mistake to touch switch 22, and the result of use is better.
Referring to fig. 2 and 3, the sealing plate 2 is further provided with a sealing stop strip 20, and a longitudinal section of the sealing stop strip 20 is larger than a longitudinal section of the opening 18. Through the sealed shelves strip 20 that sets up can enter into inside back of device case 1 at closing plate 2 completely, carry out the outer wall shutoff to opening 18 and sealing washer 19 for sealed effect is better, more is favorable to going on of experiment.
Please refer to fig. 5, the second connecting frame 4 is symmetrically provided with connecting plates 23 along the central axis thereof, the bottom of the connecting plates 23 is provided with sliding slots 24, a supporting plate 25 is disposed inside the device case 1 below the second connecting frame 4, and the top of the supporting plate 25 is provided with rollers 26 adapted to the sliding slots 24. Through the roller 26 that sets up can be at the inside roller 26 of spout 24, when second linking frame 4 moves to the device case 1 outside, and extension board 25 carries out synchronous motion thereupon, and the setting of roller 26 can make the second linking frame 4 remove more stably, is convenient for the stable transportation to cell culture dish 8.
Please refer to fig. 5, the top of the support plate 25 is symmetrically provided with electromagnetic blocks 27 along the central axis thereof, the bottom of the connecting plate 23 is provided with iron blocks 28 adapted to the electromagnetic blocks 27, and the electromagnetic blocks 27 are electrically connected to the switch 22 through wires. When the sealing plate 2 moves out of the device box 1, the switch 22 can be touched before, the electromagnetic block 27 on the left side can be powered off, the connecting plate 23 can be convenient to drive the iron block 28 to move, and after the sealing plate 2 stretches out of the device box 1 to a proper position, the iron block 28 can just move to the electromagnetic block 27 on the right side, the electromagnetic block 27 on the right side is powered on, the two are attracted, the connecting plate 23 is stabilized, namely, the sealing plate 2, the first connecting frame 3 and the second connecting frame 4 are stabilized, when the switch 22 is reused to enable the motor 13 to rotate reversely, the electromagnetic block 27 on the right side is powered off, the electromagnetic block 27 on the left side is powered on, the reciprocating mode is adopted, the sealing plate 2 can be stabilized when the final point of the inner part and the outer part of the device box 1, and the using effect is better.
Please refer to fig. 6, the top of the support net 7 is uniformly provided with placing slots 29, the inner side walls of the placing slots 29 are provided with elastic balls 30, and the cell culture dish 8 is located between the elastic balls 30. The cell culture dish 8 can be clamped by the arranged elastic ball 30, so that the cell culture dish is stable.
Example two:
referring to fig. 1 and fig. 7, the present embodiment provides an experimental apparatus for bacterial infection on a cell culture dish 8, which is different from the first embodiment in that: in the first embodiment, a coil 31 is additionally provided at the bottom of the supporting net 7, a bellows 32 is connected to one end of the coil 31, and the coil 31 is uniformly provided with atomizing nozzles 33 along the length direction thereof. The other end of the corrugated pipe 32 is connected with a structure identical to that of the top atomizing funnel in the device case 1, specifically, a pipeline, and a bacteria culture solution bottle and a compressed gas bottle respectively located at two ends of the pipeline, and the specific structure is the same as that of the above embodiment, and is not described again. Utilize this structure can make the fungus liquid spout from atomizer 33, be convenient for carry out accurate the spraying to the cell culture dish 8 on every layer, the result of use is better. The rest is the same as the first embodiment.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims (7)

1. The bacterial infection experiment device for the cell culture dish (8) comprises a device box (1) and is characterized in that an opening (18) is formed in one side of the device box (1), a sealing ring (19) is arranged on the inner side wall of the opening (18), a sealing plate (2) penetrating into the device box (1) is tightly attached to the inner wall of the sealing ring (19), a first connecting frame (3) and a second connecting frame (4) are respectively arranged at the upper end and the lower end of one side, facing the inside of the device box (1), of the sealing plate (2), a moving block (10) is fixedly arranged at the top of the first connecting frame (3) and the bottom of the second connecting frame (4), two first rotating shafts (9) are rotatably connected onto the inner wall of the device box (1) through bearings, one of the first rotating shafts (9) is positioned above the first connecting frame (3), the other first rotating shaft (9) is positioned below the second connecting frame (4), the first rotating shafts (9) penetrate through the moving block (10) and are rotatably connected with the moving block (10) through threads, one side of the first connecting frame (3) and the second connecting frame (4) are respectively connected with supporting legs, one side of the sealing plate (5), and be provided with supporting network (7) on supporting legs (6), the top of supporting network (7) is provided with cell culture dish (8), first gear (11) have still been cup jointed on first pivot (9), one side of device case (1) is provided with fixed box (12), and the internally mounted of fixed box (12) has motor (13), the output of motor (13) is connected with second pivot (14) that extend to the device box inside, and has cup jointed second gear (15) on second pivot (14), be connected through drive belt (16) transmission between first gear (11) and second gear (15), one side of device case (1) still is provided with control box (17) that are used for control motor (13) to start.
2. A cell culture dish (8) bacterial infection experimental apparatus according to claim 1, characterized in that: the device is characterized in that a through hole is formed in one side, away from the device box, of the control box (17), a baffle (21) used for plugging the through hole is hinged to the position, close to the through hole, of the control box (17) through a pin shaft, and a switch (22) electrically connected with the motor (13) is further arranged inside the control box (17).
3. A cell culture dish (8) bacterial infection experimental apparatus according to claim 1, characterized in that: the sealing plate (2) is further provided with a sealing baffle strip (20), and the longitudinal section of the sealing baffle strip (20) is larger than that of the opening (18).
4. A cell culture dish (8) bacterial infection experimental apparatus according to claim 2, characterized in that: the connecting plates (23) are symmetrically arranged on the second connecting frame (4) along the central axis of the second connecting frame, the bottom of each connecting plate (23) is provided with a sliding groove (24), a support plate (25) is arranged below the second connecting frame (4) inside the device box (1), and the top of each support plate (25) is provided with a roller (26) matched with the corresponding sliding groove (24).
5. A cell culture dish (8) bacterial infection experimental apparatus according to claim 4, characterized in that: the top of the support plate (25) is symmetrically provided with electromagnetic blocks (27) along the central axis thereof, the bottom of the connecting plate (23) is provided with iron blocks (28) matched with the electromagnetic blocks (27), and the electromagnetic blocks (27) are electrically connected with the switch (22) through conducting wires.
6. A cell culture dish (8) bacterial infection experimental apparatus according to claim 1, characterized in that: the top of the supporting net (7) is uniformly provided with placing grooves (29), the inner side walls of the placing grooves (29) are provided with elastic balls (30), and the cell culture dish (8) is located between the elastic balls (30).
7. A cell culture dish (8) bacterial infection experimental apparatus according to claim 1, characterized in that: the bottom of supporting network (7) is provided with coiled pipe (31), and the one end of coiled pipe (31) is connected with bellows (32), evenly be provided with atomizer (33) along its length direction on coiled pipe (31).
CN202222770003.6U 2022-10-20 2022-10-20 Cell culture dish bacterial infection experimental apparatus Active CN218710438U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202222770003.6U CN218710438U (en) 2022-10-20 2022-10-20 Cell culture dish bacterial infection experimental apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202222770003.6U CN218710438U (en) 2022-10-20 2022-10-20 Cell culture dish bacterial infection experimental apparatus

Publications (1)

Publication Number Publication Date
CN218710438U true CN218710438U (en) 2023-03-24

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