CN114159532A

CN114159532A - Antibacterial composition and preparation method thereof

Info

Publication number: CN114159532A
Application number: CN202111557193.7A
Authority: CN
Inventors: 彭森宏
Original assignee: Jiangxi Oxygen Beauty Biotechnology Co ltd
Current assignee: Jiangxi Oxygen Beauty Biotechnology Co ltd
Priority date: 2021-12-18
Filing date: 2021-12-18
Publication date: 2022-03-11
Also published as: CN114159532A8

Abstract

The invention provides an antibacterial composition and a preparation method thereof, and relates to the technical field of antibiosis. The antibacterial composition comprises the following raw materials in parts by weight: 30-102 parts of antibacterial, 720 parts of olive oil 600-containing, 330 parts of soybean oil 250-containing, 5-15 parts of vitamin E and 40-60 parts of ozone. The antibacterial composition has the advantages of antibacterial property, mild property and no harm to human body.

Description

Antibacterial composition and preparation method thereof

Technical Field

The invention relates to the technical field of antibiosis, and particularly relates to an antibacterial composition and a preparation method thereof.

Background

The improvement of human living standard and the convenient use of the transportation mode lead people to frequently go out for travel, gather food, shop and other public activities, so that the hands of people are easy to contact various pathogenic microorganisms such as bacteria, viruses and the like, if the pathogenic microorganisms cannot be effectively eliminated in time, the pathogenic microorganisms can survive for hours at the palm, finger joints, fingertips and other positions, and even can continue to grow and propagate to become places where the pathogenic microorganisms and the viruses are hidden and propagated. For example, contaminated hands can be used for scratching and rubbing the nose without cleaning, germs on the hands can enter the hands in a false manner, and easily enter the human body through respiratory tracts, nasal and ocular membranes and the like; the hands can take things to eat without disinfection, and microorganisms such as bacteria, viruses and the like on the hands can be transferred to food, so that people can easily infect various diseases after eating the food; before the towel is used, hands are not cleaned, germs on the hands are transferred to the towel, and when a face is washed, the towel contacts with the eye strength membrane, the nose strength membrane and the like, and the germs can enter a human body by riding the machine.

Gynecological inflammation is a common disease of women, and is caused by that many people lack due knowledge of gynecological diseases, lack of health care of bodies and various diseases caused by bad living habits, and finally, a large number of toxins and bacteria are hidden in the vagina to cause the internal environment of the vagina to be disordered. The common gynecological inflammations include female vulvitis, vaginitis, cervicitis and the like. Clinically, gynecological inflammation mainly shows that vaginal secretion is increased and is accompanied by symptoms such as peculiar smell, pruritus vulvae, leukorrhagia, burning pain and the like, and great inconvenience is brought to normal life and work.

Therefore, the antibacterial liquid has important significance for preventing germs from entering human bodies and relieving gynecological inflammation.

At present, most of antibacterial agents commonly used in China are chemical agents and the like, and the antibacterial agents cause harm to human bodies to a certain extent while sterilizing, and particularly have great harm to people with weak resistance and poor immunity, so that the antibacterial agent which is antibacterial, mild in property and capable of avoiding causing harm to human bodies is necessary.

Disclosure of Invention

The invention aims to provide an antibacterial composition which has the advantages of antibacterium, mild property and no harm to human bodies.

Another object of the present invention is to provide a method for preparing an antibacterial composition to obtain the antibacterial composition.

The technical problem to be solved by the invention is realized by adopting the following technical scheme.

In one aspect, an antibacterial composition is provided in the embodiments of the present application, and includes the following raw materials in parts by weight: 30-102 parts of antibacterial, 720 parts of olive oil 600-containing, 330 parts of soybean oil 250-containing, 5-15 parts of vitamin E and 40-60 parts of ozone.

In another aspect, the present application provides a method for preparing an antibacterial composition, including the steps of:

crushing the antibacterial, adding water, soaking for 1-3h, heating and decocting, filtering and separating to obtain filtrate and filter residue;

centrifuging the filtrate, and collecting supernatant;

drying the residue at 45-55 deg.C, adding ethanol into the dried residue, microwave extracting, steaming to remove ethanol, and concentrating to obtain extract;

adding the extract into the supernatant, adding antibacterial, oleum Olivarum, soybean oil, and vitamins, mixing, and adding ozone to obtain the final product.

Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects:

according to the invention, the antibacterial composition is prepared by using ozone, on one hand, the composition has a good sterilization effect by using the sterilization property of ozone, and the ozone is safe to human bodies and safe to use; the composition has the advantages of high safety, good antibacterial and bactericidal effects, no drug resistance and cheap raw materials by utilizing the advantages of the traditional Chinese medicine extract, such as mildness to a human body, low price and no side effect, and has good effects of relieving pain and itching and inhibiting bacteria by the synergistic effect of the medicinal materials. And as various traditional Chinese medicines can be mutually assisted, the traditional Chinese medicine has the advantages of quick response and outstanding antibacterial effect. The Chinese medicinal extract may have unstable or deteriorated active ingredients due to long-term storage, and the olive oil, the soybean oil and the vitamin E are added, so that the Chinese medicinal extract can be uniformly dispersed in the Chinese medicinal extract, the use time can be prolonged, and the skin can be moisturized.

The antibacterial composition is prepared by soaking antibacterial materials for subsequent extraction, decocting the antibacterial materials to extract a part of effective substances dissolved in water, performing centrifugal separation to remove impurities and leave pure effective substances, performing microwave extraction on filter residues to extract some water-insoluble effective substances, concentrating the water-insoluble effective substances to obtain an extract, mixing the extract with a supernatant, adding olive oil, soybean oil and vitamin E, finally flushing ozone, and flushing the ozone in to fully wrap the ozone by oily substances.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.

A bottom of the pan: bitter taste, cold nature and toxicity. Has the effects of clearing away heat and toxic materials, relieving swelling and pain. It can be used for treating swelling and pain of throat, toothache, conjunctival congestion, bacillary dysentery, enteritis, gastralgia, hepatitis, urinary tract infection, and furuncle.

Flavescent sophora root: bitter taste and cold nature. Has the effects of clearing heat, drying dampness, killing parasites and promoting urination. Can be used for treating dysentery with heat, hematochezia, jaundice, anuria, leucorrhea with red and white discharge, pudendal swelling, pruritus vulvae, eczema, skin pruritus, scabies, tinea, and leprosy; it can be used for treating trichomonas vaginitis.

Small wax trees: bitter taste and cool nature. Has the effects of clearing heat, promoting diuresis, removing toxicity and relieving swelling. It can be used for treating common cold, fever, cough due to lung heat, sore throat, aphtha, jaundice due to damp-heat, dysentery, carbuncle, suppurative sore, eczema, dermatitis, traumatic injury, and scald.

Bitter gourd: bitter taste and cold nature. Has the effects of clearing summer heat, removing heat, improving eyesight and detoxifying. It is indicated for feverish sensation with polydipsia, sunstroke, dysentery, red eye pain, carbuncle, swelling, erysipelas and malignant boil.

Honeycomb grass: pungent and bitter in flavor and mild in nature. Has the effects of detoxifying, relieving cough, improving eyesight and stimulating the menstrual flow. It can be used for treating common cold, headache, asthma, pertussis, swelling and pain of throat, toothache, dyspepsia, menoxenia, amenorrhea, wet and hard tissue, and cellulitis.

Stemona root: sweet and bitter in flavor and slightly warm in nature. Has the effects of moistening lung, descending qi, relieving cough and killing parasites. Can be used for treating chronic cough, pulmonary tuberculosis cough, and pertussis; it is used for treating head louse, body louse, enterobiasis, and pudendal pruritus.

Sweet wormwood herb: bitter and pungent in flavor and cold in nature. Has the effects of clearing away summer-heat, removing heat and steaming, and preventing malaria. Can be used for treating fever due to summer-heat, fever due to yin deficiency, night fever with early coolness, hectic fever due to yin deficiency, malaria with cold-heat, and jaundice due to damp-heat.

Groundsel: bitter, pungent and cool in nature. Has the effects of clearing away heat and toxic materials, cooling blood, relieving swelling, removing liver heat and improving eyesight. Can be used for treating acute contagious conjunctivitis, skin sore, furuncle, toxic swelling, skin eczema, dysentery, and abdominal pain.

Snow lotus herb, Tianshan: sweet and bitter in flavor and warm in nature. Has the effects of dispelling cold, strengthening yang, regulating menstruation and stopping bleeding. It can be used for treating sexual impotence, weakness of waist and knees, metrorrhagia, menoxenia, rheumatic arthritis, and traumatic hemorrhage.

Cactus and hundred grass: pungent and astringent taste, warm in nature. Has the effects of relieving swelling and pain and relieving snake venom.

Mahonia stem: bitter taste and cold nature. Has the effects of clearing heat, eliminating dampness and detoxifying. Can be used for treating cough due to lung heat, jaundice, diarrhea, dysentery, conjunctival congestion, swelling and pain, pyocutaneous disease, eczema, and scald.

Magnolia officinalis: bitter and pungent in flavor and warm in nature. Has the effects of eliminating dampness and phlegm, descending qi and relieving fullness. Can be used for treating damp stagnation, abdominal distention, vomiting, diarrhea, food stagnation, qi stagnation, abdominal distention, constipation, phlegm retention, asthma, and cough.

Clove: pungent flavor and warm nature. Has the effects of warming the middle-jiao, lowering the adverse flow of qi, tonifying the kidney and supporting yang. Can be used for treating deficiency-cold of spleen and stomach, singultus emesis, anorexia vomiting and diarrhea, psychroalgia of heart and abdomen, and sexual impotence due to kidney deficiency.

The invention provides an antibacterial composition which comprises the following raw materials in parts by weight: 30-102 parts of antibacterial, 720 parts of olive oil 600-containing, 330 parts of soybean oil 250-containing, 5-15 parts of vitamin E and 40-60 parts of ozone.

According to the invention, the antibacterial composition is prepared by using ozone, on one hand, the composition has a good sterilization effect by using the sterilization property of ozone, and the ozone is safe to human bodies and safe to use; the composition has the advantages of high safety, good antibacterial and bactericidal effects, no drug resistance and cheap raw materials by utilizing the advantages of the traditional Chinese medicine extract, such as mildness to a human body, low price and no side effect, and has good effects of relieving pain and itching and inhibiting bacteria by the synergistic effect of the medicinal materials. And as various traditional Chinese medicines can be mutually assisted, the traditional Chinese medicine has the advantages of quick response and outstanding antibacterial effect. The Chinese medicinal extract may have unstable or deteriorated active ingredients due to long-term storage, and the olive oil, the soybean oil and the vitamin E are added, so that the Chinese medicinal extract can be uniformly dispersed in the Chinese medicinal extract, the use time can be prolonged, and the skin can be moisturized. Olive oil and soybean oil are plant-extracted oils which have excellent moisturizing properties and can play a moisturizing role when sprayed on the skin. Vitamin E has excellent antioxidant effect, and can moisten skin.

In some embodiments of the present invention, the antibacterial material comprises the following raw materials in parts by weight: 1-5 parts of hemsleya root, 2-8 parts of radix sophorae flavescentis, 1-5 parts of fraxinus chinensis, 2-7 parts of bitter gourd, 3-7 parts of beehive grass, 2-8 parts of radix stemonae, 1-5 parts of sweet wormwood, 2-7 parts of groundsel, 5-10 parts of saussurea involucrate, 1-8 parts of cactus, 1-5 parts of mahonia stem, 2-7 parts of mangnolia officinalis, 1-5 parts of clove and 5-10 parts of humectant. The antibacterial agent has the synergistic effect of the medicines and has the effects of resisting and sterilizing.

In some embodiments of the present invention, the antibacterial composition comprises the following raw materials in parts by weight: 65 parts of antibacterial, 660 parts of olive oil, 290 parts of soybean oil, 10 parts of vitamin E and 50 parts of ozone.

The invention also provides a preparation method of the antibacterial composition, which comprises the following steps:

centrifuging the filtrate, and collecting supernatant;

adding the extract into the supernatant, adding antibacterial, oleum Olivarum, soybean oil, and vitamins, mixing, and adding ozone to obtain the final product. The invention firstly soaks the antibacterial material for subsequent extraction, then boils the antibacterial material to extract part of the effective substances dissolved in water, then carries out centrifugal separation to remove impurities and leave pure effective substances, and then carries out microwave extraction on the filter residue to extract some water-insoluble effective substances, then concentrates the water-insoluble effective substances to obtain extract, mixes the extract and the supernatant, adds olive oil, soybean oil and vitamin E, and finally flushes ozone to obtain the finished product.

In some embodiments of the present invention, the size of the crushed antibacterial is 50 to 150 mesh. After being crushed, the utility model can be conveniently soaked by water, and can be conveniently subsequently boiled.

In some embodiments of the present invention, the weight ratio of the water added during soaking to the crushed antibacterial is (5-10): 1.

in some embodiments of the present invention, the soaking temperature is 35-55 deg.C, and the decocting condition is heating and decocting at 95-105 deg.C for 1-2 hr.

In some embodiments of the present invention, the centrifugation is performed at 4000-.

In some embodiments of the present invention, the weight ratio of the ethanol to the dried filter residue is (10-15): 1, wherein the volume concentration of the ethanol is 65-75%.

In some embodiments of the present invention, the microwave extraction condition is that the extraction is performed for 30-60min under the conditions of microwave power of 550-850w and temperature of 65-75 ℃; the temperature of the ozone is 3-6 ℃ and the pressure is 0.25-0.35 MPa. Under this condition with ozone rush in, can make ozone fully by oily material parcel, under this condition, can guarantee when using that ozone slowly releases, disinfect to the microorganism, and can guarantee that ozone is wrapped up for a long time, stability is high.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

A method of preparing an antimicrobial composition comprising the steps of:

raw materials: antibacterial agents (10 g of hemsleya root, 20g of radix sophorae flavescentis, 10g of fraxinus chinensis, 20g of balsam pear, 30g of beehive grass, 20g of radix stemonae, 10g of sweet wormwood, 20g of groundsel, 50g of snow lotus herb, 10g of cactus, 10g of mahonia stem, 20g of mangnolia officinalis and 10g of clove), 6000g of olive oil, 2500g of soybean oil and 50g of vitamin E50 g.

The antibacterial was mixed and crushed to 50 mesh, according to 5: 1, soaking for 1h at 35 ℃, heating and decocting for 1h at 95 ℃, filtering and separating to obtain filtrate and filter residue;

centrifuging the filtrate at 4000r/min for 10min, and collecting supernatant;

drying the filter residue at 45 ℃, and mixing the filter residue according to the weight ratio of 10: 1, adding 65 volume percent ethanol into the dried filter residue, extracting for 30min under the conditions of microwave power of 550w and temperature of 65 ℃, evaporating to remove the ethanol, and concentrating to obtain an extract;

adding the extract into the supernatant, adding antibacterial, oleum Olivarum, soybean oil, and vitamins, mixing, and injecting ozone at 3 deg.C and 0.25MPa to obtain the final product.

Example 2

A method of preparing an antimicrobial composition comprising the steps of:

raw materials: antibacterial agents (20 g of hemsleya root, 30g of sophora flavescens, 20g of fraxinus chinensis, 30g of balsam pear, 40g of beehive grass, 30g of radix stemonae, 20g of sweet wormwood, 30g of groundsel, 60g of snow lotus herb, 20g of cactus, 20g of mahonia stem, 30g of mangnolia officinalis, 20g of clove), 6300g of olive oil, 2800g of soybean oil and 80g of vitamin E80 g.

The antibacterial was mixed and crushed to 100 mesh, as per 6: 1, soaking for 2 hours at 40 ℃, heating and decocting for 1.5 hours at 100 ℃, and filtering and separating to obtain filtrate and filter residue;

centrifuging the filtrate at 4500r/min for 13min, and collecting supernatant;

drying the filter residue at 50 ℃, and mixing the filter residue according to the weight ratio of 11: 1, adding ethanol with the volume concentration of 70 percent into the dried filter residue, extracting for 40min under the conditions that the microwave power is 650w and the temperature is 70 ℃, evaporating to remove the ethanol, and concentrating to obtain an extract;

adding the extract into the supernatant, adding antibacterial, oleum Olivarum, soybean oil, and vitamins, mixing, and injecting ozone at 4 deg.C and 0.3MPa to obtain the final product.

Example 3

A method of preparing an antimicrobial composition comprising the steps of:

raw materials: antibacterial agents (30 g of hemsleya root, 50g of sophora flavescens, 30g of fraxinus chinensis, 50g of balsam pear, 50g of beehive grass, 50g of radix stemonae, 30g of sweet wormwood, 50g of groundsel, 80g of snow lotus herb, 40g of cactus, 30g of mahonia stem, 50g of mangnolia officinalis and 30g of clove) 6600g of olive oil, 2900g of soybean oil and 100g of vitamin E100 g.

The antibacterial was mixed and crushed to 100 mesh, according to 8: 1, soaking for 2 hours at 45 ℃, heating and decocting for 1.5 hours at 100 ℃, and filtering and separating to obtain filtrate and filter residue;

centrifuging the filtrate at 4500r/min for 13min, and collecting supernatant;

drying the filter residue at 50 ℃, and mixing the filter residue according to the weight ratio of 13: 1, adding ethanol with volume concentration of 70% into the dried filter residue, extracting for 50min under the conditions of microwave power of 750w and temperature of 70 ℃, evaporating to remove the ethanol, and concentrating to obtain an extract;

Example 4

A method of preparing an antimicrobial composition comprising the steps of:

raw materials: antibacterial agents (40 g of hemsleya root, 60g of sophora flavescens, 40g of fraxinus chinensis, 60g of balsam pear, 60g of beehive grass, 60g of radix stemonae, 40g of sweet wormwood, 60g of groundsel, 90g of snow lotus herb, 60g of cactus, 40g of mahonia stem, 60g of magnolia officinalis and 40g of clove), 7000g of olive oil, 3000g of soybean oil and 120g of vitamin E120 g.

The antibacterial was mixed and crushed to 100 mesh, according to 9: 1, soaking for 2.5h at 50 ℃, heating and decocting for 1.5h at 100 ℃, and filtering and separating to obtain filtrate and filter residue;

centrifuging the filtrate at 4800r/min for 14min, and collecting supernatant;

drying the filter residue at 50 ℃, and mixing the filter residue according to the weight ratio of 14: 1, adding ethanol with volume concentration of 70% into the dried filter residue, extracting for 50min under the conditions of microwave power of 750w and temperature of 70 ℃, evaporating to remove the ethanol, and concentrating to obtain an extract;

adding the extract into the supernatant, adding antibacterial, oleum Olivarum, soybean oil, and vitamins, mixing, and injecting ozone at 5 deg.C and 0.33MPa to obtain the final product.

Example 5

A method of preparing an antimicrobial composition comprising the steps of:

raw materials: antibacterial agents (50 g of hemsleya root, 80g of sophora flavescens, 50g of fraxinus chinensis, 70g of balsam pear, 70g of beehive grass, 80g of radix stemonae, 50g of sweet wormwood, 70g of groundsel, 100g of snow lotus herb, 80g of cactus, 50g of mahonia stem, 70g of mangnolia officinalis and 50g of clove), 7200g of olive oil, 3300g of soybean oil and 150g of vitamin E150 gg.

The antibacterial was mixed and crushed to 150 mesh, according to 10: 1, adding water, soaking at 55 ℃ for 3h, heating and decocting at 105 ℃ for 2h, and filtering and separating to obtain filtrate and filter residue;

centrifuging the filtrate at 5000r/min for 15min, and collecting supernatant;

drying the filter residue at 55 ℃, and mixing the filter residue according to the weight ratio of 15: 1, adding 75 percent ethanol into the dried filter residue by weight, extracting for 60min under the conditions that the microwave power is 850w and the temperature is 75 ℃, evaporating to remove the ethanol, and concentrating to obtain an extract;

adding the extract into the supernatant, adding antibacterial, oleum Olivarum, soybean oil, and vitamins, mixing, and injecting ozone at 6 deg.C and 0.35MPa to obtain the final product.

Comparative example 1

This comparative example is essentially the same as example 3, except that: radix Hemsleyae Macrospermae and radix et caulis Opuntiae Dillenii are not added.

A method of preparing an antimicrobial composition comprising the steps of:

raw materials:

antibacterial agents (50 g of radix sophorae flavescentis, 30g of fraxinus chinensis, 50g of bitter gourd, 50g of beehive grass, 50g of radix stemonae, 30g of sweet wormwood, 50g of groundsel, 80g of snow lotus herb, 30g of mahonia stem, 50g of mangnolia officinalis and 30g of clove) 6600g of olive oil, 2900g of soybean oil and 100g of vitamin E.

centrifuging the filtrate at 4500r/min for 13min, and collecting supernatant;

Comparative example 2

This comparative example is essentially the same as example 3, except that: radix Sophorae Flavescentis, caulis Mahoniae and herba Senecionis Scandentis are not added.

A method of preparing an antimicrobial composition comprising the steps of:

raw materials:

antibacterial materials (30 g of radix Hemsleyae Macrospermae, 30g of cercaria chinensis, 50g of balsam pear, 50g of beehive grass, 50g of radix Stemonae, 30g of sweet wormwood herb, 80g of snow lotus herb, 40g of cactus, 50g of officinal magnolia bark and 30g of clove) olive oil 6600g, soybean oil 2900g and vitamin E100 g.

centrifuging the filtrate at 4500r/min for 13min, and collecting supernatant;

Examples of the experiments

First, verification of bacteriostatic effect

Escherichia coli, Staphylococcus aureus and Candida albicans stored on a 4 ℃ slant were inoculated into a sterilized nutrient agar medium, and the antibacterial compositions prepared in examples 1 to 5 and comparative examples 1 to 2 were injected into the surface of the nutrient agar medium with a 5 mm-diameter punch, respectively, and cultured in a 37 ℃ water-area incubator for 24 hours, and the diameters of the zones were measured, and the results are shown in Table 1.

TABLE 1

According to table 1, it can be seen that the antibacterial composition prepared by the present application has a good bacteriostatic effect on bacteria.

Second, skin irritation verification

21 white rabbits with the weight of 2 +/-0.2 kg and randomly divided into 7 groups were selected, and the antibacterial composition prepared in examples 1-5 and the antibacterial composition prepared in comparative examples 1-2 were respectively used for testing, the two sides of the spine of each rabbit were cut off by 3cm × 3cm before the test, the antibacterial composition was applied to the skin on one side, the other side was used as a control group, the application was performed 1 time per day for 30 days, the skin was washed with warm water after each application for 4 hours, and the results were observed after each application for 24 hours, and the skin of 7 groups of rabbits did not change after each application for 30 days, which indicates that the antibacterial composition prepared in the present application has no irritation to the skin.

In conclusion, the antibacterial composition is prepared by using the ozone, so that on one hand, the composition has a good sterilization effect by using the sterilization property of the ozone, and the ozone is safe to human bodies and safe to use; the composition has the advantages of high safety, good antibacterial and bactericidal effects, no drug resistance and cheap raw materials by utilizing the advantages of the traditional Chinese medicine extract, such as mildness to a human body, low price and no side effect, and has good effects of relieving pain and itching and inhibiting bacteria by the synergistic effect of the medicinal materials. And as various traditional Chinese medicines can be mutually assisted, the traditional Chinese medicine has the advantages of quick response and outstanding antibacterial effect. The Chinese medicinal extract may have unstable or deteriorated active ingredients due to long-term storage, and the olive oil, the soybean oil and the vitamin E are added, so that the Chinese medicinal extract can be uniformly dispersed in the Chinese medicinal extract, the use time can be prolonged, and the skin can be moisturized.

The invention firstly soaks the antibacterial material for subsequent extraction, then boils the antibacterial material to extract part of the effective substances dissolved in water, then carries out centrifugal separation to remove impurities and leave pure effective substances, and then carries out microwave extraction on the filter residue to extract some water-insoluble effective substances, then concentrates the water-insoluble effective substances to obtain extract, mixes the extract and the supernatant, adds olive oil, soybean oil and vitamin E, and finally flushes ozone to obtain the finished product.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims

1. The antibacterial composition is characterized by comprising the following raw materials in parts by weight: 29-97 parts of antibacterial, 720 parts of olive oil 600-containing, 330 parts of soybean oil 250-containing, 5-15 parts of vitamin E and 40-60 parts of ozone.

2. The antibacterial composition according to claim 1, wherein the antibacterial material comprises the following raw materials in parts by weight: 1-5 parts of hemsleya root, 2-8 parts of radix sophorae flavescentis, 1-5 parts of fraxinus chinensis, 2-7 parts of bitter gourd, 3-7 parts of beehive grass, 2-8 parts of radix stemonae, 1-5 parts of sweet wormwood, 2-7 parts of groundsel, 5-10 parts of saussurea involucrate, 1-8 parts of cactus, 1-5 parts of mahonia stem, 2-7 parts of mangnolia officinalis, 1-5 parts of clove and 5-10 parts of humectant.

3. The antibacterial composition according to claim 2, characterized by comprising the following raw materials in parts by weight: 65 parts of antibacterial, 660 parts of olive oil, 290 parts of soybean oil, 10 parts of vitamin E and 50 parts of ozone.

4. A process for the preparation of an antimicrobial composition according to any one of claims 1 to 3, comprising the steps of:

centrifuging the filtrate, and collecting supernatant;

5. The method of claim 4, wherein the size of the crushed antibacterial material is 50-150 mesh.

6. The method for preparing the antibacterial composition according to claim 4, wherein the weight ratio of the water added during soaking to the crushed antibacterial material is (5-10): 1.

7. the method for preparing the antibacterial composition according to claim 4, wherein the soaking temperature is 35-55 ℃, and the boiling condition is heating and boiling at 95-105 ℃ for 1-2 h.

8. The method as claimed in claim 4, wherein the centrifugation is carried out at 4000-.

9. The method for preparing the antibacterial composition according to claim 4, wherein the weight ratio of the ethanol to the dried filter residue is (10-15): 1, wherein the volume concentration of the ethanol is 65-75%.

10. The method as claimed in claim 4, wherein the microwave extraction is performed at a microwave power of 550 and 850w and a temperature of 65-75 ℃ for 30-60 min; the temperature of the ozone is 3-6 ℃ and the pressure is 0.25-0.35 MPa.