CN104958400A - Oculentum for treating acute conjunctivitis and preparation method thereof - Google Patents

Abstract

The invention discloses oculentum for treating acute conjunctivitis and a preparation method thereof. The oculentum is prepared from the following raw medicaments: coptis, radix scutellariae, lac, alpine yarrow herb, Chinese mahonia leaf, hylocereus undatus, purslane, pomegranate bark, honey-fried licorice root, Mongolian snake gourd root, fructus arctii, peach, borneol flakes, vinegar, chrysanthemum lavandulifolium and olive oil. The invention overcomes the defect that the existing antibiotic ophthalmic external medicament for treating acute conjunctivitis easily results in tolerance and side effects, and provides the oculentum for treating acute conjunctivitis and the preparation method thereof; by clearing heat, removing blood stasis, diminishing inflammation and relieving pain, both symptoms and root causes of the acute conjunctivitis are treated; the oculentum is a pure Chinese medicinal preparation, is mild and non-irritant, directly acts on the affected part to kill pathogenic bacteria, can quickly relieve ophthalmic causalgia of patients, and has good anti-bacterial and anti-inflammatory effects and significant treatment effect.

Description

A kind of eye ointment for the treatment of acute conjunctivitis and preparation method thereof

Technical field

The present invention relates to the field of Chinese medicines, particularly a kind of eye ointment for the treatment of acute conjunctivitis and preparation method thereof.

Background technology

Under normal circumstances, conjunctiva has certain defence capability, but when defence capability weakens or extraneous paathogenic factor increases, conjunctival tissue inflammation will be caused to occur, and this inflammation is referred to as conjunctivitis.Super acute, acute, subacute, chronic conjunctivitis can be divided into by the course of disease.A kind of common acute Epidemic ophthalmic that acute conjunctivitis is often caused by bacteriological infection.Common pathogenic bacterium Staphylococcus pneumoniae, Koch-Weeks bacillus, staphylococcus epidermidis, staphylococcus aureus and streptococcus etc.By the article (as towel, handkerchief, washbowl etc.) that contact patient's eyes secretions or tear were stained with, shaking hands with patient or rub eyes etc. with dirty hands all can be infected.Summer and autumn, because of heat, the easy growth and breeding of antibacterial, is very easy to cause and is very popular.The principal character of this disease is: conjunctiva is hyperemia, purulence or mucosa purulent secretion obviously, suffer from ophthalmic pruritus and have foreign body sensation, have time serious that eyelid is heavy, photophobia is shed tears and burning sensation, sometimes because secretions is attached to anterior corneal surface lesser ring of Merkel, cause temporary blurring of vision, can vision be recovered after flushing, when pathological changes invade and cornea time can have photophobia, ophthalmalgia and obviously increase the weight of depending on the symptom such as to go down, small number of patients can have upper respiratory tract infection or other General Symptomies simultaneously.If treated not in time, some acute conjunctivitiss can change into chronic conjunctivitis.This disease treated by antibiotic ophthalmic liquid medicine or the eye ointment of adopting more at present clinically, but, use antibiotic not only can produce drug resistance, also likely cause other side effect, cause other diseases, making patients is painful, chloromycetin as treated acute conjunctivitis conventional can cause optic neuritis ataxia, photis, use in continuous 1 month also can cause toxic amblyopia, optic atrophy etc., and the irritated patient of part, eye drip also can cause anaphylaxis conjunctivitis.Use antibiotic ophthalmic liquid medicine that eyes also can be made to produce irritating effect, the ill symptoms such as cause that eyes are often shed tears.The traditional Chinese medical science is thought, acute conjunctivitis system is by experiencing ailment said due to cold or exposure pyretic toxicity, and invasion and attack human body eye causes.

Summary of the invention

The present invention improves prior art, in treatment acute conjunctivitis, the defect of drug resistance and side effect is easily produced in order to overcome existing antibiotics eye medicine for external use, there is provided a kind of eye ointment for the treatment of acute conjunctivitis and preparation method thereof, this eye ointment is pure Chinese medicinal preparation, and formula gentleness is non-stimulated, directly act on affected part, safety has no side effect, and can not produce drug resistance, can rapid recovery eye causalgia symptom, anti-inflammation is effective, evident in efficacy.

To achieve these goals, technical scheme provided by the invention is as follows:

Treat an eye ointment for acute conjunctivitis, crude drug composition and the parts by weight of making described eye ointment are: Rhizoma Coptidis 8-10 part, Radix Scutellariae 10-12 part, Lacca 8-10 part, achillea millefolium 5-7 part, Folium Mahoniae 6-8 part, Hylocereus undatus 2-4 part, Herba Portulacae 12-15 part, Pericarpium Granati 6-8 part, Radix Glycyrrhizae Preparata 8-10 part, Radix Trichosanthis 5-7 part, Fructus Arctii 8-10 part, Fructus Persicae 15-20 part, Blumeae preparatum Tabellae 5-8 part, vinegar 3-5 part, Flos Chrysanthemi 6-8 part, olive oil 30-40 part.

Further, crude drug composition and the parts by weight of making described eye ointment are: Rhizoma Coptidis 8 parts, Radix Scutellariae 10 parts, Lacca 8 parts, achillea millefolium 5 parts, Folium Mahoniae 6 parts, Hylocereus undatus 2 parts, Herba Portulacae 12 parts, Pericarpium Granati 6 parts, Radix Glycyrrhizae Preparata 8 parts, Radix Trichosanthis 5 parts, Fructus Arctii 8 parts, Fructus Persicae 15 parts, Blumeae preparatum Tabellae 5 parts, vinegar 3 parts, Flos Chrysanthemi 6 parts, 30 parts, olive oil.

Further, crude drug composition and the parts by weight of making described eye ointment are: Rhizoma Coptidis 10 parts, Radix Scutellariae 12 parts, Lacca 10 parts, achillea millefolium 7 parts, Folium Mahoniae 8 parts, Hylocereus undatus 4 parts, Herba Portulacae 15 parts, Pericarpium Granati 8 parts, Radix Glycyrrhizae Preparata 10 parts, Radix Trichosanthis 7 parts, Fructus Arctii 10 parts, Fructus Persicae 20 parts, Blumeae preparatum Tabellae 8 parts, vinegar 5 parts, Flos Chrysanthemi 8 parts, 40 parts, olive oil.

Further, crude drug composition and the parts by weight of making described eye ointment are: Rhizoma Coptidis 9 parts, Radix Scutellariae 11 parts, Lacca 9 parts, achillea millefolium 6 parts, Folium Mahoniae 7 parts, Hylocereus undatus 3 parts, Herba Portulacae 14 parts, Pericarpium Granati 7 parts, Radix Glycyrrhizae Preparata 9 parts, Radix Trichosanthis 6 parts, Fructus Arctii 9 parts, Fructus Persicae 17 parts, Blumeae preparatum Tabellae 7 parts, vinegar 4 parts, Flos Chrysanthemi 7 parts, 35 parts, olive oil.

The invention also discloses the preparation method of the eye ointment of described treatment acute conjunctivitis, comprise the following steps:

(1) Rhizoma Coptidis, Radix Scutellariae, Folium Mahoniae, Radix Trichosanthis, Fructus Arctii, Lacca, achillea millefolium, Hylocereus undatus, Pericarpium Granati, Radix Glycyrrhizae Preparata, Flos Chrysanthemi add gross mass multiple 6-8 water soaking doubly 1 hour, slow fire boiling 2 hours, cross 500 order drainage screens after decocting, separating filtrate, filtering residue are for subsequent use;

(2) step (1) gained filtering residue puts into 80 DEG C, baking oven oven dry 45 minutes, and the dry thing super micron mill of gained is pulverized, and crosses 2000 mesh sieves and obtains mixed-powder, for subsequent use;

(3) Fructus Persicae enucleation, mix with Herba Portulacae and clean, add after the mixing of step (1) gained filtrate is squeezed the juice and leave standstill 2 hours, cross 200 order filter screen filtration, the concentrated oven dry of gained filtrate is the concentrated solution of 1.20 ± 0.02 (60 DEG C) to relative density;

(4) in the concentrated solution of step (3), add the mixed-powder that step (2) is obtained, then add olive oil, Blumeae preparatum Tabellae, vinegar fully stirs, mix homogeneously, must treat the eye ointment of acute conjunctivitis.

The traditional Chinese medical science is thought, acute conjunctivitis system is by experiencing ailment said due to cold or exposure pyretic toxicity, invasion and attack human body eye causes, and the present invention, in order to treat acute conjunctivitis, takes the method for heat clearing away eliminating stagnation, anti-inflammatory analgetic, for the cause of disease and this disease for the treatment of for diseases of this disease, according to Chinese medical theory determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, screen raw material of Chinese medicine by great many of experiments, refining agent method, make each efficacy of drugs produce synergism, thus reach the effect for the treatment of both the principal and secondary aspects of a disease.In the crude drug chosen: Rhizoma Coptidis: bitter in the mouth, cold, heat clearing and damp drying, eliminating fire and detoxication, anti-inflammation, for damp and hot feeling of fullness, vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood tells nosebleed, conjunctival congestion, toothache, quench one's thirst, carbuncle furuncle, external treatment eczema, eczema, auditory meatus is suppurated, it is burning hot that Rhizoma Coptidis (processed with wine) is apt to the clear part of the body cavity above the diaphragm housing the heart and lungs, for conjunctival congestion, aphtha, prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting, tie mutually for cold and heat, damp and hot middle resistance, feeling of fullness is vomitted, cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting, for incoordination between the liver and stomach, vomiting acid regurgitation, Rhizoma Coptidis and Radix Glycyrrhizae are share can the effect of obvious its anti-Staphylococcus aureus of gain, Radix Scutellariae: bitter in the mouth, cold in nature, there are heat clearing and damp drying, eliminating fire and detoxication, hemostasis, the effect such as antiabortive, cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle, the clinical antibiotic property of Radix Scutellariae is better than Rhizoma Coptidis, and does not develop immunity to drugs, Lacca: bitter, flat, clearing away heat,cooling blood and removing toxin, removing blood stasis with potent drugs promoting the circulation of QI to relieve pain, Lacca can detoxify and invigorate blood circulation, and again without the trouble that work rushes down, this is that it surpasses the place of Radix Arnebiae (Radix Lithospermi), achillea millefolium: bitter, acid, flat, anti-inflammation, removing toxic substances analgesia, with controlling mastitis, acute tonsillitis, appendicitis, pyelitis etc., rheumatalgia, traumatic injury, innominate toxic swelling, venom etc., have inhibitory action to staphylococcus aureus, dysentery bacterium, escherichia coli, bacillus pyocyaneus, Folium Mahoniae: bitter, cool, heat clearing away tonify deficiency, removing pathogenic heat from blood and toxic substance from the body, double circulation of qi promoting divides, and preventing phlegm from forming and stopping coughing cures mainly hemoptysis of pulmonary tuberculosis, osteopyrexia and fever, dizziness and tinnitus, lassitude loin and legs, vexed conjunctival congestion, the diseases such as anemopyretic cold, Hylocereus undatus: cold nature, sweet in the mouth, clearing heat and moistening lung, control the hot xeropulmonary cough of expectorant, Herba Portulacae: cold in nature, sweet in the mouth acid, enters the heart, liver, spleen, large intestine channel, have that removing dampness is antipruritic, clearing heat for detumescence, heat-clearing and toxic substances removing, diuretic dries, loose blood detumescence, dust-collection sterilization, anti-inflammatory analgetic, the effect of hemostasis removing heat from blood, Herba Portulacae has stronger inhibitory action to dysentery bacterium, Bacillus typhi and escherichia coli, can be used for the auxiliary treatment of various inflammation, have the title of " natural antibiotics ", simultaneously Herba Portulacae is also rich in vitamin C, and vitamin C has good antioxidation can whitening nourishes rough skin, Pericarpium Granati: convergence, antibacterial.In vitro tests has antibacterial action to bacillus pyocyaneus, bacterium flexneri, Bacillus typhi, also has inhibitory action in various degree to various skin fungus.Also has resisiting influenza virus effect, Radix Glycyrrhizae Preparata: containing number of chemical compositions such as glycyrrhizin, enoxolone, Angelica Polysaccharide, there is antiinflammatory and anti-allergy action, scalable body's immunity, antitumor and analgesic effect, therefore the primary efficacy of Radix Glycyrrhizae Preparata is and middle emergency, lung moistening, removing toxic substances, coordinating the actions of various ingredients in a prescription, Radix Trichosanthis: clearing away heat and promoting production of body fluid, detumescence and apocenosis, for calentura excessive thirst, lung-heat type cough, interior-heat is quenched one's thirst, sore swollen toxin, Fructus Arctii: dispelling wind and heat pathogens, lung qi dispersing rash, subduing swelling and detoxicating, controls cough due to pathogenic wind-heat, laryngopharynx swelling and pain, and macule is not saturating, and rubella is had an itch, carbuncle sore tumefacting virus, Fructus Persicae: sweet in the mouth acid, warm in nature, there is tonifying QI to produce body fluid, the merit of promoting blood circulation to remove stagnancy, hinder dryness of the intestine constipation for Tianjin, blood stasis lump, hepatosplenomegaly etc., Blumeae preparatum Tabellae: there is refreshment of having one's ideas straightened out, effect of clearing away heat to alleviate pain, is usually used in calentura coma, convulsion faints, apoplexy syncope due to accumulation of phlegm, stagnation of QI sudden syncope, and attacked by pestiferous factors is gone into a coma, conjunctival congestion, aphtha, laryngopharynx swelling and pain, vinegar: bitter, temperature, dissipating blood stasis removing food stagnancy, hemostasis, relieving colic caused by ascaris, removing toxic substances, main puerperal blood faints, lump in the abdomen, spit blood, epistaxis, have blood in stool, abdominal pain due to worm stagnation, flesh of fish dish poison, carbuncle sore tumefacting virus, control puerperal blood faints, Xuan Pi mass in the abdomen, jaundice, yellowish perspiration, spit blood, epistaxis, stool hematochezia, pudendal pruritus, carbuncle skin ulcer swells, separate flesh of fish dish poison, acetic acid has well antibacterial and bactericidal action, to common streptococcus pneumoniae, staphylococcus, influenza virus etc. have inhibitory action, modern scientific research proves the acetic acid in vinegar, lactic acid, aminoacid, the compound such as glycerol and aldehyde, soft stimulation is had to skin, thin vessels is expanded, increase the blood circulation of skin, sour environment contributes to medicine transdermal, vinegar also plays chemical modification effect to effective ingredient, medicine physicochemical property can be changed, salt is formed with alkaloids, its water solublity is increased thus the skin distribution behavior of change drug molecule, Flos Chrysanthemi: bitter in the mouth, pungent, cold nature, GUIXIN, spleen, Liver Channel, have improving eyesight, move back liver-fire, except effect of wind heat, the liver benefiting nourishing YIN, olive oil: be rich in monounsaturated fatty acid, has the effect of antiinflammatory, to blood vessel and joint all beneficial.

In all medicines, Herba Portulacae and Rhizoma Coptidis are monarch drug, play the effect of heat clearing away eliminating stagnation, anti-inflammatory analgetic altogether, for this disease of cause of disease treatment for diseases.Radix Scutellariae, Lacca, achillea millefolium, Folium Mahoniae, Hylocereus undatus, Pericarpium Granati, Radix Trichosanthis, Fructus Arctii, Fructus Persicae, olive oil are ministerial drug; Wherein, Radix Scutellariae, Lacca, achillea millefolium, Folium Mahoniae, Hylocereus undatus, Radix Trichosanthis, Fructus Arctii, Fructus Persicae can the effect of gain monarch drug heat clearing away eliminating stagnation, removes ailment said due to cold or exposure pyretic toxicity, effectively improves the symptom of conjunctival congestion and secretions increase; Radix Scutellariae, achillea millefolium, Folium Mahoniae, Pericarpium Granati, olive oil and monarch drug play the effect that antiinflammatory relieves the pain altogether, and auxiliary monarch drug effectively kills pathogenic bacterium fast, and rapid recovery eye causalgia, alleviates patient suffering.Radix Glycyrrhizae Preparata, Blumeae preparatum Tabellae, vinegar are assistant, Radix Glycyrrhizae Preparata plays harmonizing yingfen and weifen, the effect of warming spleen and stomach for dispelling cold, simultaneously, Rhizoma Coptidis and Radix Glycyrrhizae are share can the effect of obvious its anti-Staphylococcus aureus of gain, Blumeae preparatum Tabellae and vinegar can promote the Transdermal absorption of effective ingredient, promote that drug effect plays, simultaneously vinegar also can gain monarch-minister drug anti-inflammatory analgetic, promoting blood circulation to remove obstruction in the collateral effect, Blumeae preparatum Tabellae also has the effect of heat-clearing and toxic substances removing, this Chinese medicinal lotion does not adopt conventional ethanol to promote drug absorption, and adopts gentleer a small amount of vinegar and Blumeae preparatum Tabellae to work the effect promoting Transdermal absorption, and formula more safety is gentle.Flos Chrysanthemi, for drawing, can reach order by priming, better treat ocular disease, effectively alleviates the symptom of acute conjunctivitis.Eye ointment of the present invention is pure Chinese medicinal preparation, selected medical material compatibility is suitable, directly act on affected part, the gentle safety of formula, and adopt modern advanced medical pharmaceutical technology to make, and there is treating both the principal and secondary aspects of a disease rapid-action, can effective reduction of patient misery, the remarkable advantage of safe without toxic side effect, is adapted to the treatment to acute conjunctivitis.

Beneficial effect of the present invention is: instant invention overcomes the defect that existing antibiotics eye medicine for external use easily produces drug resistance and side effect in treatment acute conjunctivitis, provide a kind of eye ointment for the treatment of acute conjunctivitis and preparation method thereof, adopt heat clearing away eliminating stagnation, the method that antiinflammatory relieves the pain, for this disease of cause of disease disease treating both the principal and secondary aspects of a disease, this eye ointment is pure Chinese medicinal preparation, formula gentleness is non-stimulated, adopt unguentum dosage form, long period can directly act on affected part, effectively kill pathogenic bacterium, safe without toxic side effect, drug resistance can not be produced, can rapid recovery eye causalgia symptom, anti-inflammation is effective, evident in efficacy.This eye ointment adopts a small amount of vinegar and Blumeae preparatum Tabellae to promote Transdermal absorption, and formula is safety temperature more.Flos Chrysanthemi, for drawing, can reach order by priming, better treat ocular disease, effectively alleviates the symptom of acute conjunctivitis.

Detailed description of the invention

According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only should can not limit the present invention described in detail in claims yet for illustration of the present invention.

Embodiment 1

Treat a Chinese medicinal lotion for acute conjunctivitis, raw material composition and the parts by weight of making described Chinese medicinal lotion are: Rhizoma Coptidis 6 parts, Radix Scutellariae 7 parts, Lacca 8-part, achillea millefolium 5 parts, Folium Mahoniae 4 parts, Hylocereus undatus 3 parts, Herba Portulacae 15 parts, Pericarpium Granati 5 parts, Radix Glycyrrhizae Preparata 7 parts, Radix Trichosanthis 4 parts, Fructus Arctii 8 parts, Fructus Persicae 15 parts, Blumeae preparatum Tabellae 5 parts, vinegar 3 parts, Flos Chrysanthemi 8 parts; The raw material of described Chinese medicinal lotion also comprises sodium chloride and the appropriate phosphate buffer (pH buffer) of parts by weight 4 parts.

The preparation method of the eye ointment of described treatment acute conjunctivitis, comprises the following steps:

(1) Rhizoma Coptidis, Radix Scutellariae, Folium Mahoniae, Radix Trichosanthis, Fructus Arctii, Lacca, achillea millefolium, Hylocereus undatus, Pericarpium Granati, Radix Glycyrrhizae Preparata, Flos Chrysanthemi add gross mass multiple 6-8 water soaking doubly 1 hour, slow fire boiling 2 hours, cross 500 order drainage screens after decocting, separating filtrate, filtering residue are for subsequent use;

(2) step (1) gained filtering residue puts into 80 DEG C, baking oven oven dry 45 minutes, and the dry thing super micron mill of gained is pulverized, and crosses 2000 mesh sieves and obtains mixed-powder, for subsequent use;

(3) Fructus Persicae enucleation, mix with Herba Portulacae and clean, add after the mixing of step (1) gained filtrate is squeezed the juice and leave standstill 2 hours, cross 200 order filter screen filtration, the concentrated oven dry of gained filtrate is the concentrated solution of 1.20 ± 0.02 (60 DEG C) to relative density;

(4) in the concentrated solution of step (3), add the mixed-powder that step (2) is obtained, then add olive oil, Blumeae preparatum Tabellae, vinegar fully stirs, mix homogeneously, must treat the eye ointment of acute conjunctivitis.

Embodiment 2

Treat a Chinese medicinal lotion for acute conjunctivitis, crude drug composition and the parts by weight of making described Chinese medicinal lotion are: Rhizoma Coptidis 8 parts, Radix Scutellariae 9 parts, Lacca 10 parts, achillea millefolium 7 parts, Folium Mahoniae 6 parts, Hylocereus undatus 5 parts, Herba Portulacae 19 parts, Pericarpium Granati 7 parts, Radix Glycyrrhizae Preparata 9 parts, Radix Trichosanthis 6 parts, Fructus Arctii 10 parts, Fructus Persicae 20 parts, Blumeae preparatum Tabellae 8 parts, vinegar 5 parts, Flos Chrysanthemi 10 parts; The raw material of described Chinese medicinal lotion also comprises sodium chloride and the appropriate phosphate buffer (pH buffer) of parts by weight 6 parts.

The preparation method of the Chinese medicinal lotion of embodiment 2 is identical with embodiment 1.

Embodiment 3

Treat a Chinese medicinal lotion for acute conjunctivitis, crude drug composition and the parts by weight of making described Chinese medicinal lotion are: Rhizoma Coptidis 7 parts, Radix Scutellariae 8 parts, Lacca 9 parts, achillea millefolium 6 parts, Folium Mahoniae 5 parts, Hylocereus undatus 4 parts, Herba Portulacae 17 parts, Pericarpium Granati 6 parts, Radix Glycyrrhizae Preparata 8 parts, Radix Trichosanthis 5 parts, Fructus Arctii 9 parts, Fructus Persicae 17 parts, Blumeae preparatum Tabellae 7 parts, vinegar 4 parts, Flos Chrysanthemi 9 parts; The raw material of described Chinese medicinal lotion also comprises sodium chloride and the appropriate phosphate buffer (pH buffer) of parts by weight 5 parts.

The preparation method of the Chinese medicinal lotion of embodiment 3 is identical with embodiment 1.

Embodiment 4 irritation test

Get the embodiment of the present invention and certain commercially available collyrium, the irritant reaction situation to conjunctiva, cornea and iris after observation animal eyes contact test sample.

(cornea is without muddiness to get the undamaged healthy rabbits of examination of eyes 12, conjunctiva is without hyperemia, edema and secretions, pupil is circular, both sides etc. are large, good to luminous reflectance), be divided into 4 groups at random by body weight, 3/group, 1st group of left eye makes own control to normal saline, and right eye is to erythromycin spongaion.2-4 group left eye also makes own control to normal saline, and right eye is to the eye ointment of embodiment 1-3.Respectively eye ointment is applied in rabbit eyelid, compressing nasolacrimal duct, and makes eyes passive closed about 5-10 second.2 times/day, each 0.5g, continuous eye drip 14 days, before each administration and after last administration 1, 2, 4, 24, 48 and 72 hours, direct perusal or observe cornea with magnifier, iris, whether conjunctiva is irritant, and zest is marked, in good time use 2% fluorescein sodium dyeing, whether observe each position has painted, result carries out irritant reaction scoring by table 1, by the cornea of each of each rabbit observing time, the irritant reaction score value of iris and conjunctiva is added to obtain total mark, by the integration summation of a group divided by number of animals, obtain last mean scores, and compare with left eye.Then, by evaluation criterion judgement sample to eye irritation.When the stimulus intensity of cornea, iris, conjunctiva is inconsistent, evaluation should be made respectively.Wherein table 1 is Eye irritation reaction score criteria, and table 2 is eye irritation evaluation criterions, and table 3 is eye irritation experimental results.

Table 1 Eye irritation reaction score criteria

Table 2 eye irritation evaluation criterion

Score value	0-3	4-8	9-12	13-16
					Evaluate	Nonirritant	Slight zest	Moderate zest	Intensity zest

Table 3 eye irritation experimental result

Known by animal eye stimulation test result, the eye ointment of embodiment of the present invention 1-3 and normal saline, to the equal nonirritant of rabbit eye, illustrate that eye ointment formula gentleness provided by the invention is safe and non-stimulating.

Embodiment 5 bacteriostatic experiment

1, experimental strain: staphylococcus aureus

2, bacterium culture medium: nutrient agar and nutrient broth medium.

3, bacteriostatic experiment divides three experimental grouies, a positive controls and negative control group, experimental group uses the eye ointment of embodiment of the present invention 1-3, wherein, experimental group A is the unguentum of gained in embodiment 1, experimental group B is the unguentum of gained in embodiment 2, experimental group C is the unguentum of gained in embodiment 3, and positive controls selects erythromycin spongaion (the accurate word H44023089 of traditional Chinese medicines), and negative control group is blank.

4, specific experiment step:

(1) susceptibility sense sheet preparation: the round scraps of paper that diameter 6mm made by two circle board qualitative filter paper card punch put into clean dry penicillin empty bottle, bottleneck is wrapped up with monolayer kraft paper, 121 DEG C of autoclaving 30min, then through 100 DEG C of oven dry, airtightly saves backup.Aseptically respectively the scraps of paper are soaked in 2h in the eye ointment of embodiment 1 to 3 and erythromycin spongaion, after medicinal liquid is absorbed by the scraps of paper completely, take out filter paper, it is for subsequent use that susceptibility sheet is made in 50 DEG C of oven dry, often 5 susceptibility sense sheets prepared by kind of a unguentum, prepare the scraps of paper of 5 blank sterilizings as negative control group simultaneously;

(2) prepare bacterium liquid: staphylococcus aureus strain is placed in 10ml nutrient broth medium, cultivate 18h for 37 DEG C; Get 0.9% aseptic sodium chloride solution that culture fluid 1ml adds 9ml, adopt 10 times and increase progressively dilution method, be diluted to 105 ~ 106cfu/ml, for subsequent use;

(3) the test organisms liquid that above-mentioned steps (2) is obtained is evenly inoculated on mannitol sodium chloride agar plate, left at room temperature 5min, 20 susceptibility sense sheets obtained in step (1) and 5 blank sterilizing scraps of paper are laid on this agar plate, flat board to be inverted in incubator 37 DEG C cultivate 24h after, measure the antibacterial circle diameter (mm) of each susceptibility sheet, calculate the antibacterial circle diameter meansigma methods of each group, above operation is sterile working.

5, experimental observation is measured:

Observe: represent with "-" without inhibition zone;

Antibacterial circle diameter < 10mm is slightly antibacterial, represents with "+";

Antibacterial circle diameter 10 ~ 14mm is that moderate is antibacterial, represents with " ++ ";

Antibacterial circle diameter > 14mm is highly antibacterial, represents with " +++ ";

	Negative control	Positive control	Experimental group A	Experimental group B	Experimental group C
						Inhibitory action	-	+++	+++	+++	+++

Experimental result shows, the Main Pathogenic Bacteria staphylococcus aureus of eye ointment of the present invention to acute conjunctivitis has significant inhibitory action, and its effect is close with erythromycin spongaion.

Embodiment 5 clinical trial:

Random selecting 150 acute conjunctivitis patients carry out clinical trial, patient age 12-40 year, average 30 years old, patient is divided into 3 groups at random, Chinese medicinal lotion respectively in embodiment 1 to 3 on probation treats this disease, using method is: be applied in eyelid by this unguentum 2 times on the one, uses for the last time before sleeping.Within 5th, be a course for the treatment of, investigate the curative effect of instant particles as Chinese medicine of the present invention, the evaluation criterion of curative effect is:

Recovery from illness: symptom disappears completely, conjunctiva without congested phenomenon, without thick secretions.

Take a turn for the better: symptom disappears substantially, congested and secretions symptom obviously alleviates.

Invalid: all diseases are without improvement.

Statistical result is as follows:

	Embodiment 1	Embodiment 2	Embodiment 3
				Recovery from illness	45	43	46
Take a turn for the better	4	7	4
				Invalid	1	0	0
Effective percentage (%)	98	100	100

All patients are all without bad reflection during medication, and experimental result shows, the effective percentage of Chinese medicine lotion of the present invention reaches more than 98%, can rapid recovery patients symptomatic, evident in efficacy, can safe and effective treatment acute conjunctivitis, without recurrence after curing, it is desirable medicine.

Clinical exemplary embodiments:

1, ten thousand certain, 33 years old, man, morbidity is seeking medical attention after 2 days, symptom is conjunctival congestion, and eye has foreign body sensation, twinge, eye portion secretions, use the eye ointment treatment of the embodiment of the present invention 1, treatment eye pain on the same day, foreign body sensation, conjunctival congestion symptom were obviously alleviated, after the course for the treatment of, whole disease disappears, and drug withdrawal is interior without recurrence for January.

2, Xue so-and-so, 26 years old, female, morbidity's seeking medical attention on the same day, symptom is conjunctival congestion, eye has foreign body sensation, twinge, uses the eye ointment treatment of the embodiment of the present invention 2, treatment eye pain on the same day, foreign body sensation, conjunctival congestion symptom are obviously alleviated, after the course for the treatment of, whole disease disappears, and drug withdrawal is interior without recurrence for January.

3, week certain, 19 years old, female, morbidity's seeking medical attention on the 2nd, symptom is that eye twinge has foreign body sensation, conjunctival congestion, eye portion secretions, use the eye ointment of the embodiment of the present invention 3, treatment eye twinge foreign body sensation on the same day and conjunctival congestion symptom are obviously alleviated, and after the course for the treatment of, whole disease disappears, patient fully recovers, and drug withdrawal is interior without recurrence for January.

Above embodiment is only for illustrating technical conceive of the present invention and feature; its object is to allow person skilled in the art understand content of the present invention and to be implemented; can not limit the scope of the invention with this; all equivalences done according to spirit of the present invention change or modify, and all should be encompassed in protection scope of the present invention.

Claims (5)

1. treat an eye ointment for acute conjunctivitis, it is characterized in that: crude drug composition and the parts by weight of making described eye ointment are: Rhizoma Coptidis 8-10 part, Radix Scutellariae 10-12 part, Lacca 8-10 part, achillea millefolium 5-7 part, Folium Mahoniae 6-8 part, Hylocereus undatus 2-4 part, Herba Portulacae 12-15 part, Pericarpium Granati 6-8 part, Radix Glycyrrhizae Preparata 8-10 part, Radix Trichosanthis 5-7 part, Fructus Arctii 8-10 part, Fructus Persicae 15-20 part, Blumeae preparatum Tabellae 5-8 part, vinegar 3-5 part, Flos Chrysanthemi 6-8 part, olive oil 30-40 part.

2. a kind of eye ointment for the treatment of acute conjunctivitis as claimed in claim 1, is characterized in that: crude drug composition and the parts by weight of making described eye ointment are: Rhizoma Coptidis 8 parts, Radix Scutellariae 10 parts, Lacca 8 parts, achillea millefolium 5 parts, Folium Mahoniae 6 parts, Hylocereus undatus 2 parts, Herba Portulacae 12 parts, Pericarpium Granati 6 parts, Radix Glycyrrhizae Preparata 8 parts, Radix Trichosanthis 5 parts, Fructus Arctii 8 parts, Fructus Persicae 15 parts, Blumeae preparatum Tabellae 5 parts, vinegar 3 parts, Flos Chrysanthemi 6 parts, 30 parts, olive oil.

3. a kind of eye ointment for the treatment of acute conjunctivitis as claimed in claim 1, is characterized in that: crude drug composition and the parts by weight of making described eye ointment are: Rhizoma Coptidis 10 parts, Radix Scutellariae 12 parts, Lacca 10 parts, achillea millefolium 7 parts, Folium Mahoniae 8 parts, Hylocereus undatus 4 parts, Herba Portulacae 15 parts, Pericarpium Granati 8 parts, Radix Glycyrrhizae Preparata 10 parts, Radix Trichosanthis 7 parts, Fructus Arctii 10 parts, Fructus Persicae 20 parts, Blumeae preparatum Tabellae 8 parts, vinegar 5 parts, Flos Chrysanthemi 8 parts, 40 parts, olive oil.

4. a kind of eye ointment for the treatment of acute conjunctivitis as claimed in claim 1, is characterized in that: crude drug composition and the parts by weight of making described eye ointment are: Rhizoma Coptidis 9 parts, Radix Scutellariae 11 parts, Lacca 9 parts, achillea millefolium 6 parts, Folium Mahoniae 7 parts, Hylocereus undatus 3 parts, Herba Portulacae 14 parts, Pericarpium Granati 7 parts, Radix Glycyrrhizae Preparata 9 parts, Radix Trichosanthis 6 parts, Fructus Arctii 9 parts, Fructus Persicae 17 parts, Blumeae preparatum Tabellae 7 parts, vinegar 4 parts, Flos Chrysanthemi 7 parts, 35 parts, olive oil.

5., as a kind of preparation method for the treatment of the eye ointment of acute conjunctivitis of Claims 1-4 as described in any one, it is characterized in that: comprise the following steps:

(1) Rhizoma Coptidis, Radix Scutellariae, Folium Mahoniae, Radix Trichosanthis, Fructus Arctii, Lacca, achillea millefolium, Hylocereus undatus, Pericarpium Granati, Radix Glycyrrhizae Preparata, Flos Chrysanthemi add gross mass multiple 6-8 water soaking doubly 1 hour, slow fire boiling 2 hours, cross 500 order drainage screens after decocting, separating filtrate, filtering residue are for subsequent use;

(2) step (1) gained filtering residue puts into 80 DEG C, baking oven oven dry 45 minutes, and the dry thing super micron mill of gained is pulverized, and crosses 2000 mesh sieves and obtains mixed-powder, for subsequent use;

(3) Fructus Persicae enucleation, mix with Herba Portulacae and clean, add after the mixing of step (1) gained filtrate is squeezed the juice and leave standstill 2 hours, cross 200 order filter screen filtration, the concentrated oven dry of gained filtrate is the concentrated solution of 1.20 ± 0.02 (60 DEG C) to relative density;

(4) in the concentrated solution of step (3), add the mixed-powder that step (2) is obtained, then add olive oil, Blumeae preparatum Tabellae, vinegar fully stirs, mix homogeneously, must treat the eye ointment of acute conjunctivitis.