CN105943718B - Agilawood compound traditional Chinese medicine composition as well as preparation method and application thereof - Google Patents
Agilawood compound traditional Chinese medicine composition as well as preparation method and application thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The invention relates to the technical field of traditional Chinese medicine preparations, and in particular relates to an agilawood compound traditional Chinese medicine composition as well as a preparation method and application thereof. The traditional Chinese medicine composition comprises an agilawood extract, a cassia seed extract, a honeysuckle flower extract and a mulberry leaf extract. The traditional Chinese medicine composition adopts proper raw material proportions, perfectly combines the effects of the components, fully exerts the effects of the traditional Chinese medicine components, has better effect than that of each single preparation, can relieve eye fatigue, prevent and treat eye diseases, has the effects of resisting bacteria, diminishing inflammation and the like, does not irritate eyes, has elegant fragrance, and makes people delightful.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine preparations, and in particular relates to an agilawood compound traditional Chinese medicine composition as well as a preparation method and application thereof.
Background
With the continuous development of modern science and technology, various electronic products are changing day by day, and many people can use products such as computers, televisions, mobile phones and the like in daily life and work, so that when watching the computers, the televisions or the mobile phones for a long time with full attention, the eye blinking frequency is reduced, the tear secretion is correspondingly reduced, and meanwhile, the blinking screen strongly stimulates the eyes, and the eyes can also cause dryness, discomfort, serious symptoms and even pain. Excessive eye use can cause vision loss, and is accompanied by symptoms such as easy fatigue of visual objects, soreness and discomfort of eyes and the like. Meanwhile, due to the continuous development of the industry, the living environment and the air of people are polluted to a certain extent, and the eyes are easily inflamed due to the fact that people sometimes pay attention to sanitation and the like. Therefore, the eye drops with the efficacies of relieving eyestrain, preventing and treating dry eyes, resisting bacteria and diminishing inflammation and the like become daily standby medicines for people.
The eye drops for relieving eye dryness sold in the market at present mainly comprise polyvinyl alcohol, can be used as a lubricant for improving eye dryness, but cannot play the roles of relieving eye fatigue, resisting bacteria and diminishing inflammation. Some eye drops contain preservatives, and damage to corneal epithelium is easily caused by long-term use, resulting in xerophthalmia. Eye drops used to treat eye infections often contain antibiotics, are susceptible to bacterial infections, and can cause blindness due to glaucoma with abuse. The use of ophthalmic solutions containing steroids over extended periods of time can result in increased intraocular pressure. Moreover, most of the eye drops have certain irritation and have certain side effect on eyes after long-term use.
Compared with eye drops containing western medicine components, the eye drops containing Chinese herbal medicine components have higher safety. However, the Chinese herbal medicine eye drops sold in the market at present have poor curative effects on relieving eye fatigue, preventing and treating dry eyes, resisting bacteria and diminishing inflammation. Therefore, there is still a need for a Chinese herbal medicine eye drop which has better curative effect and no irritation.
Disclosure of Invention
In view of the above, the invention provides an agilawood compound traditional Chinese medicine composition, and a preparation method and application thereof. The traditional Chinese medicine composition adopts proper raw material proportions, perfectly combines the effects of the components, fully exerts the effects of the traditional Chinese medicine components, can relieve eye fatigue, prevent and treat eye diseases, has the effects of resisting bacteria, diminishing inflammation and the like, has no stimulation to eyes, has elegant fragrance and makes people delightful.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a traditional Chinese medicine composition which comprises an agilawood extract, a cassia seed extract, a honeysuckle extract and a mulberry leaf extract.
Agilawood: has pungent and bitter taste and mild nature, has effects of activating qi-flowing, relieving pain, warming middle energizer, relieving vomit, and receiving qi and relieving asthma, and can be used for treating chest and abdomen distention, pain, stomach cold, emesis, singultus, deficiency of the kidney, asthma, etc. The Chinese eaglewood decoction has different degrees of inhibition effects on tubercle bacillus, shigella flexneri and typhoid bacillus of human bodies; the main components of the lignum Aquilariae Resinatum, namely sesquiterpene and 2- (2-phenylethyl) chromone, have bacteriostatic effects of inhibiting Staphylococcus aureus, Candida albicans, agricultural pathogenic bacteria, etc., and the 2- (2-phenylethyl) chromone has antiallergic effects of different degrees.
Cassia seed: is dry mature seed of Cassia tora or Cassia tora L.of Leguminosae, which is named for its eyesight improving effect, and is bitter, sweet, salty and slightly cold in nature. Enter liver, kidney and large intestine meridians. Clear liver and improve vision, moisten intestines and relax bowels. Can be used for treating conjunctival congestion, photophobia, lacrimation, headache, vertigo, dim eyesight, and constipation.
Honeysuckle flower: is cold in nature and sweet in taste, enters lung, heart and stomach meridians, and has the effects of clearing away heat and toxic materials and resisting inflammation. Honeysuckle is well known for its wide medicinal value since ancient times. The efficacy of the traditional Chinese medicine is mainly to clear away heat and toxic materials and mainly treat epidemic febrile disease, fever, heat toxin, bloody dysentery, carbuncle, cellulitis, furuncle and the like. Modern researches prove that the honeysuckle contains pharmacological active ingredients such as chlorogenic acid and luteolin glycoside, has strong inhibitory effect on various pathogenic bacteria such as hemolytic streptococcus and staphylococcus aureus and upper respiratory infection pathogenic viruses, and can enhance immunity, resist early pregnancy, protect liver, resist tumor, diminish inflammation, relieve fever, stop bleeding (blood coagulation), inhibit intestinal absorption of cholesterol and the like, and has very wide clinical application.
Mulberry leaf: bitter and cold, entering liver meridian, has the action of pacifying and descending liver yang, so it can be used to treat liver yang hyperactivity, headache and vertigo; disperse wind-heat, clear liver fire, nourish yin, cool blood and improve vision.
After the agilawood extract, the cassia seed extract, the honeysuckle extract and the mulberry leaf extract are combined for use, the antibacterial effect is obvious, and the effect is obviously better than that of each single agent.
Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 1-25 parts of agilawood extract, 1-15 parts of semen cassiae extract, 1-15 parts of honeysuckle extract and 1-15 parts of mulberry leaf extract.
In some embodiments provided by the invention, the amount of each component in the traditional Chinese medicine composition is as follows by weight: 25 parts of agilawood extract, 13 parts of semen cassiae extract, 12 parts of honeysuckle extract and 10 parts of mulberry leaf extract.
The invention also provides application of the traditional Chinese medicine composition in preparing medicines for relieving eye fatigue, preventing and treating dry eyes and/or resisting bacteria and diminishing inflammation.
The invention also provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
respectively carrying out alcohol extraction and concentration on agilawood, semen cassiae, honeysuckle and mulberry leaves to obtain extractum, obtaining agilawood extract, semen cassiae extract, honeysuckle extract and mulberry leaf extract, and mixing the agilawood extract, semen cassiae extract, honeysuckle extract and mulberry leaf extract to obtain the traditional Chinese medicine composition.
Preferably, the concentration of ethanol used for alcohol extraction is not less than 70%.
In some embodiments provided herein, the ethanol concentration is 95%.
Preferably, the alcohol extraction times are 1-5, and the time of each alcohol extraction is 48-72 h.
In some embodiments provided by the present invention, the number of times of alcohol extraction is 3, and the time for each alcohol extraction is 72 hours.
The invention also provides eye drops which comprise the traditional Chinese medicine composition, hyaluronic acid, an antioxidant and a phosphate buffer solution.
Preferably, the traditional Chinese medicine composition comprises, by weight, 10-30 parts of a traditional Chinese medicine composition, 10-30 parts of hyaluronic acid, 1-10 parts of an antioxidant and 10-30 parts of a phosphate buffer solution.
In some embodiments provided by the invention, the traditional Chinese medicine composition comprises 20 parts by weight, 30 parts by weight of hyaluronic acid, 5 parts by weight of antioxidant and 30 parts by weight of phosphate buffer.
The invention also provides a preparation method of the eye drops, which comprises the following steps:
mixing the traditional Chinese medicine composition, hyaluronic acid, an antioxidant and a phosphate buffer solution, standing for 5-10 hours, and filtering to obtain the eye drops.
In some embodiments provided herein, the time of standing is 6 hours.
The invention provides an agilawood compound traditional Chinese medicine composition and a preparation method and application thereof. The traditional Chinese medicine composition comprises an agilawood extract, a cassia seed extract, a honeysuckle flower extract and a mulberry leaf extract. The traditional Chinese medicine composition adopts proper raw material proportions, perfectly combines the effects of the components, fully exerts the effects of the traditional Chinese medicine components, has better effect than that of each single preparation, can relieve eye fatigue, prevent and treat eye diseases, has the effects of resisting bacteria, diminishing inflammation and the like, does not irritate eyes, has elegant fragrance, and makes people delightful.
Detailed Description
The invention discloses an agilawood compound traditional Chinese medicine composition as well as a preparation method and application thereof, and a person skilled in the art can realize the compound traditional Chinese medicine composition by properly improving process parameters by referring to the content. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The agilawood compound traditional Chinese medicine composition, and the preparation method and the raw material medicines or auxiliary materials used in the application of the composition can be purchased from the market. Wherein, the antioxidant adopts sodium bisulfite, and the phosphate buffer is sodium phosphate buffer (pH7.4).
The invention is further illustrated by the following examples:
example 1 Compound Chinese medicinal composition of lignum Aquilariae Resinatum and its preparation
A compound Chinese medicinal composition comprises lignum Aquilariae Resinatum:
25 parts of agilawood extract, 13 parts of semen cassiae extract, 12 parts of honeysuckle extract and 10 parts of mulberry leaf extract.
The preparation method comprises the following steps:
(1) respectively extracting lignum Aquilariae Resinatum, semen Cassiae, flos Lonicerae, and folium Mori with 95% ethanol for 3 times, each for 72 hr, and concentrating the extracts to obtain lignum Aquilariae Resinatum extract, semen Cassiae extract, flos Lonicerae extract, and folium Mori extract;
(2) and uniformly mixing the agilawood extract, the cassia seed extract, the honeysuckle extract and the mulberry leaf extract according to the prescription amount to obtain the agilawood compound traditional Chinese medicine composition.
Example 2 Chinese eaglewood compound medicine composition and preparation thereof
A compound Chinese medicinal composition comprises lignum Aquilariae Resinatum:
20 parts of agilawood extract, 1 part of semen cassiae extract, 15 parts of honeysuckle extract and 1 part of mulberry leaf extract.
The preparation method comprises the following steps:
(1) respectively extracting lignum Aquilariae Resinatum, semen Cassiae, flos Lonicerae, and folium Mori with 95% ethanol for 3 times (60 hr each time), and concentrating the extracts to obtain lignum Aquilariae Resinatum extract, semen Cassiae extract, flos Lonicerae extract, and folium Mori extract;
(2) and uniformly mixing the agilawood extract, the cassia seed extract, the honeysuckle extract and the mulberry leaf extract according to the prescription amount to obtain the agilawood compound traditional Chinese medicine composition.
Example 3 Compound Chinese medicinal composition comprising lignum Aquilariae Resinatum and its preparation
A compound Chinese medicinal composition comprises lignum Aquilariae Resinatum:
1 part of agilawood extract, 15 parts of semen cassiae extract, 1 part of honeysuckle extract and 15 parts of mulberry leaf extract.
The preparation method comprises the following steps:
(1) respectively extracting lignum Aquilariae Resinatum, semen Cassiae, flos Lonicerae, and folium Mori with 95% ethanol for 3 times, 48 hr each time, and concentrating the extracts to obtain lignum Aquilariae Resinatum extract, semen Cassiae extract, flos Lonicerae extract, and folium Mori extract;
(2) and uniformly mixing the agilawood extract, the cassia seed extract, the honeysuckle extract and the mulberry leaf extract according to the prescription amount to obtain the agilawood compound traditional Chinese medicine composition.
EXAMPLE 4 Aquilaria compound eye drops and preparation thereof
An agilawood compound eye drop comprises the following components:
example 1, 20 parts of compound agilawood traditional Chinese medicine composition, 30 parts of hyaluronic acid, 5 parts of antioxidant and 30 parts of phosphate buffer are added;
the preparation method comprises the following steps:
(1) mixing the above Chinese medicinal composition and hyaluronic acid, adding antioxidant and phosphate buffer solution, mixing, and stirring;
(2) standing the mixed solution for 6 hours, and filtering with a microporous filter membrane to obtain the eye drops.
EXAMPLE 5 Aquilaria compound eye drops and preparation thereof
An agilawood compound eye drop comprises the following components:
example 2, 30 parts of compound agilawood traditional Chinese medicine composition, 10 parts of hyaluronic acid, 10 parts of antioxidant and 10 parts of phosphate buffer are added;
the preparation method comprises the following steps:
(1) mixing the above Chinese medicinal composition and hyaluronic acid, adding antioxidant and phosphate buffer solution, mixing, and stirring;
(2) standing the mixed solution for 6 hours, and filtering with a microporous filter membrane to obtain the eye drops.
EXAMPLE 6 Compound eye drops containing lignum Aquilariae Resinatum and preparation thereof
An agilawood compound eye drop comprises the following components:
example 3 compound Chinese eaglewood composition 10 parts, hyaluronic acid 20 parts, and then antioxidant 1 part, phosphate buffer 15 parts;
the preparation method comprises the following steps:
(1) mixing the above Chinese medicinal composition and hyaluronic acid, adding antioxidant and phosphate buffer solution, mixing, and stirring;
(2) standing the mixed solution for 6 hours, and filtering with a microporous filter membrane to obtain the eye drops.
Example 7 bacteriostatic experiments
Strain: staphylococcus aureus, Escherichia coli, and pneumococcus provided by China Committee for culture Collection of microorganisms.
Sample preparation: the agilawood compound traditional Chinese medicine composition and the eye drops in the embodiment are prepared in embodiments 1 and 4; each single Chinese medicinal preparation is the alcohol extract obtained in example 1.
The method comprises the following steps: the method adopts a Sha's culture medium in-vitro drug-based method. Sterilizing the culture medium with high pressure steam at 121 deg.C for 15min, cooling to 50 deg.C, adding the lignum Aquilariae Resinatum compound Chinese medicinal composition, eye drop and erythromycin ointment prepared in examples 1 and 4 into each test tube culture medium, mixing, respectively inoculating the 3 strains onto two groups of medicinal culture media with different concentrations, placing into incubator, observing for 2 weeks, and repeating the experiment for 3 times.
The antibacterial effects of the agilawood compound traditional Chinese medicine composition and the eye drops prepared in the embodiments 1 and 4 are the same, and the results are shown in tables 1 and 2. The bacteriostatic action of erythromycin eye drops is shown in table 3. The bacteriostatic action of each single traditional Chinese medicine is shown in tables 4-7. The Minimum Inhibitory Concentration (MIC) of Staphylococcus aureus, Escherichia coli and pneumococcus of the agilawood compound traditional Chinese medicine composition in the embodiment 1 is 0.5-1.25 g/L, the MIC of the agarwood compound eye drop prepared in the embodiment 4 is 25-100 mg/L, the MIC of the agilawood extract is 1.25-2.5 g/L, the MIC of the cassia seed extract is 1.25-5.0 g/L, the MIC of the honeysuckle extract is 1.25-2.5 g/L, and the MIC of the mulberry leaf extract is 1.25-2.5 g/L, so that the antibacterial effect is reliable, the effect of the traditional Chinese medicine composition is better than that of a single traditional Chinese medicine, and the traditional Chinese medicine composition can be used as a treatment method of ophthalmic inflammation.
TABLE 1 antibacterial action of Chinese eaglewood compound medicine composition
Note: -shows bacteriostasis, + shows growth, and MIC is 0.5-1.25 g/L.
TABLE 2 antibacterial action of lignum Aquilariae Resinatum compound eye drops
Note: -shows bacteriostasis, + shows growth, and MIC is 1.0-1.25 g/L.
TABLE 3 bacteriostatic action of erythromycin eye drops
Note: -shows bacteriostasis, + shows growth, and MIC is 25-100 mg/L.
TABLE 4 antibacterial action of Aquilaria agallocha extract
Note: -shows bacteriostasis, + shows growth, and MIC is 1.25-2.5 g/L.
TABLE 5 bacteriostatic action of Cassia Torae semen extract
Note: -shows bacteriostasis, + shows growth, and MIC is 1.25-5.0 g/L.
TABLE 6 bacteriostatic action of honeysuckle extract
Note: -shows bacteriostasis, + shows growth, and MIC is 1.25-2.5 g/L.
TABLE 7 bacteriostatic action of mulberry leaf extract
Note: -shows bacteriostasis, + shows growth, and MIC is 1.25-2.5 g/L.
The traditional Chinese medicine compositions in the embodiments 2 and 3 are used for carrying out bacteriostasis tests, and the effect is equivalent to the bacteriostasis effect of the traditional Chinese medicine composition in the embodiment 1. The eye drops of examples 5 and 6 were subjected to a bacteriostatic test, and the effect thereof was comparable to that of the eye drops of example 4.
EXAMPLE 8 irritation test of Compound traditional Chinese medicine eye drops containing lignum Aquilariae Resinatum
Test animals: 2.0-2.5 Kg of New Zealand rabbit.
Single-dose eye irritation test: 0.1mL of the compound Chinese medicine eye drops of the 4 rd embodiment is dripped into the conjunctival sac of the right eye of the rabbit, and 0.1mL of the solvent is used as a control on the other side of the eye drops of the 5 healthy white rabbits without eye diseases. The rabbit eyelids were closed for 10 seconds after dosing. Recording the local reaction conditions of the rabbits 6, 24, 48, 72 to 7 days after the agilawood compound traditional Chinese medicine eye drops are given.
As a result: the right eye into which the compound traditional Chinese medicine eye drops are dropped is not obviously changed after the medicine is taken. The Chinese eaglewood compound traditional Chinese medicine eye drops are proved to have no irritation when being dropped into eyes once.
Multiple dose eye irritation test: 0.1mL of the compound Chinese medicine eye drops of the 4 rd embodiment of the invention is dripped into the conjunctival sac of the right eye of the rabbit when 8 healthy white rabbits without eye diseases are taken. The rabbit eyelids were closed for 10 seconds after dosing. And taking 0.1mL of solvent as a control on the other side, recording the local reaction condition of the rabbits 1 hour after the agilawood compound traditional Chinese medicine eye drops are given for the last time every day, and simultaneously observing the general reaction. The preparation is applied to the eye 1 time per hour, 6 times per day, and 7 days. After stopping the drug, the observation is carried out for 7 days.
As a result: in the process of seven days after administration and seven days after withdrawal, the common physical signs of the rabbits, such as diet, activity, defecation, mental state and the like, are not obviously affected.
And (4) conclusion: the results of single and multiple administrations show that: after the compound Chinese medicine eye drops are applied for one time and multiple times, the rabbit shows normal performance, and the abnormality does not occur after the application is stopped, which shows that the continuous application of the compound Chinese medicine Chinese eaglewood eye drops has no obvious irritation.
The invention utilizes the agilawood in the eye drops for the first time, not only can relieve eye fatigue and prevent and treat eye diseases, but also has elegant fragrance and enables people to be delightful.
The eye drops have the advantages of proper compatibility of selected medicinal materials, accordance with the traditional Chinese medicine and modern medicine theory, antibacterial and anti-inflammatory effects, no irritation to eyes, and normal work and life during the administration.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A Chinese medicinal composition with effects of relieving eye fatigue, preventing and treating dry eyes and/or resisting bacteria and diminishing inflammation is characterized by comprising an agilawood extract, a cassia seed extract, a honeysuckle extract and a mulberry leaf extract;
the traditional Chinese medicine composition comprises the following components in parts by weight: 1-25 parts of agilawood extract, 1-15 parts of semen cassiae extract, 1-15 parts of honeysuckle extract and 1-15 parts of mulberry leaf extract;
the preparation method comprises the following steps:
respectively carrying out alcohol extraction and concentration on agilawood, semen cassiae, honeysuckle and mulberry leaves to obtain extractum, obtaining an agilawood extract, a semen cassiae extract, a honeysuckle extract and a mulberry leaf extract, and mixing the agilawood extract, the semen cassiae extract, the honeysuckle extract and the mulberry leaf extract to obtain the traditional Chinese medicine composition.
2. The traditional Chinese medicine composition according to claim 1, wherein the amount of each component in the traditional Chinese medicine composition is as follows in parts by weight: 25 parts of agilawood extract, 13 parts of semen cassiae extract, 12 parts of honeysuckle extract and 10 parts of mulberry leaf extract.
3. The use of the Chinese medicinal composition according to claim 1 or 2 in the preparation of medicaments for relieving eyestrain, preventing and treating dry eyes and/or antibacterial and anti-inflammatory medicaments.
4. The method for preparing the traditional Chinese medicine composition according to claim 1 or 2, which is characterized by comprising the following steps:
respectively carrying out alcohol extraction and concentration on agilawood, semen cassiae, honeysuckle and mulberry leaves to obtain extractum, obtaining an agilawood extract, a semen cassiae extract, a honeysuckle extract and a mulberry leaf extract, and mixing the agilawood extract, the semen cassiae extract, the honeysuckle extract and the mulberry leaf extract to obtain the traditional Chinese medicine composition.
5. The process according to claim 4, wherein the ethanol is extracted with a concentration of not less than 70%.
6. An eye drop comprising the Chinese medicinal composition of claim 1 or 2, hyaluronic acid, an antioxidant and a phosphate buffer.
7. The eye drop according to claim 6, wherein the traditional Chinese medicine composition comprises, by weight, 10-30 parts of hyaluronic acid, 1-10 parts of antioxidant and 10-30 parts of phosphate buffer.
8. The eye drop according to claim 7, wherein the traditional Chinese medicine composition comprises 20 parts by weight of hyaluronic acid, 30 parts by weight of antioxidant and 30 parts by weight of phosphate buffer.
9. A process for the preparation of ophthalmic solutions according to any one of claims 6 to 8, characterized in that it comprises the following steps:
mixing the traditional Chinese medicine composition of claim 1 or 2, hyaluronic acid, an antioxidant and a phosphate buffer, standing for 5-10 h, and filtering to obtain eye drops.
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