CN114129495A

CN114129495A - Anti-inflammatory and allergy-relieving repairing composition and preparation method and application thereof

Info

Publication number: CN114129495A
Application number: CN202111508063.4A
Authority: CN
Inventors: 陈勇; 邱威; 巩军华; 王玉辉; 顾正平; 罗兴义; 张昕
Original assignee: Beijing Underproved Medical Technology Co ltd
Current assignee: Beijing Underproved Medical Technology Co ltd
Priority date: 2021-12-10
Filing date: 2021-12-10
Publication date: 2022-03-04
Anticipated expiration: 2041-12-10
Also published as: CN114129495B

Abstract

The invention relates to an anti-inflammatory and allergy-relieving repairing composition, which comprises the following active ingredients: cactus extract; oat bran extract; radix Sophorae Flavescentis extract; an extract of the root of Ophiopogon japonicus; radix Paeoniae extract; scutellariae radix extract; extract of Quillaja saponaria Molina. The anti-inflammatory and allergy-relieving repair composition disclosed by the invention is matched with the Quillaja saponaria Molina extract and other plant extracts, so that the anti-inflammatory, anti-allergy and analgesic effects can be improved, the skin barrier function can be enhanced, the self-protection capability of the skin can be improved, the inflammatory skin, especially the skin with hormone-dependent dermatitis can be effectively repaired, and the drug resistance, the dependence and the side effects cannot be generated after long-term use.

Description

Anti-inflammatory and allergy-relieving repairing composition and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-inflammatory and allergy-relieving repairing composition as well as a preparation method and application thereof.

Background

The skin is the largest organ in the human body, and is in direct contact with the external environment, and has the effects of protecting, excreting, regulating body temperature, sensing external stimuli and the like. The dermatitis includes allergic dermatitis, seborrheic dermatitis, and hormonal dermatitis. Allergic dermatitis is an inflammatory reaction of the skin caused by many factors. Common allergic dermatitis is mainly classified into two types: contact dermatitis and cosmetic dermatitis. Allergic reactions caused by different pathogenic factors are different. Among them, irritant contact dermatitis is the most common skin disorder using cosmetics (cleansing agents, pigments and astringents for the face), and has symptoms such as irritation, itching, redness, swelling, etc., and in severe cases, swelling and peeling of the skin may occur. Allergic contact dermatitis occurs less frequently than irritant contact dermatitis. Cosmetic ingredients that can cause skin irritation include fragrances, preservatives and sunscreens, such as irritation, itching, redness, swelling and even blistering of the skin. The adverse effects of hair dyes, the use of hair dyes can cause skin allergic reactions and skin irritation. Symptoms of these reactions include redness, ulcers, itching, burning and discomfort.

Skin allergy is an abnormal specific immune response, also called allergy, which is mainly physiological dysfunction or tissue cell damage when the body is stimulated by certain specific antigens. The symptoms of skin allergy include red swelling, itching, dryness, desquamation and the like, which not only affect the beauty, but also greatly affect the daily life of people. In recent years, the number of skin allergy patients has increased dramatically, and skin allergy has become a health problem of global concern. Skin allergies may be caused by a number of factors, such as: skin is exposed to allergens such as germs, pollen, parasites, etc.; sudden changes in weather; hormonal dyssecretion in humans; the pressure is too great.

Chinese granted patent CN110200830B discloses an anti-inflammatory and allergy-relieving repairing composition, its preparation method and application, the composition is composed of cactus extract, oat bran extract, sophora flavescens extract, ophiopogon root extract, peony root extract, scutellaria root extract, butylene glycol, pentanediol and water, its preparation method is as follows: 1) preparing a sophora flavescens extract solution; 2) homogenizing radix et caulis Opuntiae Dillenii extract, oat bran extract, radix Ophiopogonis extract, radix Paeoniae extract, Scutellariae radix extract, butanediol and pentanediol at ultrahigh pressure to obtain plant extract mixed solution; 3) mixing the plant extract mixed solution and the sophora flavescens extract solution, homogenizing under ultrahigh pressure and filtering. The anti-inflammatory and allergy-relieving repairing composition has the effects of diminishing inflammation, resisting allergy and easing pain, can enhance the barrier function of skin, improves the self-protection capability of the skin, effectively repairs inflammatory skin, particularly skin with hormone-dependent dermatitis, and does not generate drug resistance, dependence and side effect after long-term use.

However, there is room for further improvement in the anti-inflammatory effect of the anti-inflammatory and soothing repair composition disclosed in the above-identified issued patent. The present invention has been completed through further development based on the disclosure of the above-mentioned issued patent.

Disclosure of Invention

Based on the background technology, the technical problem to be solved by the invention is to provide an anti-inflammatory and allergy-relieving repairing composition, and a preparation method and application thereof. In order to realize the purpose of the invention, the following technical scheme is adopted:

in one aspect, the present invention relates to an anti-inflammatory, soothing, repairing composition comprising the following:

cactus extract; active ingredient

Oat bran extract;

radix Sophorae Flavescentis extract;

an extract of the root of Ophiopogon japonicus;

radix Paeoniae extract;

scutellariae radix extract;

extract of Quillaja saponaria Molina.

In a preferred embodiment of the present invention, the active ingredients are contained in the following mass percentages:

cactus extract: 1% -15%;

oat bran extract: 1% -5%;

and (3) sophora flavescens extract: 1% -5%;

radix ophiopogonis extract: 1% -5%;

peony root extract: 1% -5%;

radix scutellariae extract: 1% -5%;

duck Quillaja saponaria extract: 1 to 3 percent.

In a preferred embodiment of the invention, the weight percentage of the duck quillaja extract is 1.5-2.5%.

cactus extract: 2% -4%;

oat bran extract: 2% -4%;

and (3) sophora flavescens extract: 2% -4%;

radix ophiopogonis extract: 2% -4%;

peony root extract: 2% -4%;

radix scutellariae extract: 2% -4%;

duck Quillaja saponaria extract: 1.8 to 2.2 percent.

The extract of the duck quillaja used in the present invention contains 50 wt% or more of acacetin, which is a natural gum derivative known to exert a skin-firming effect in sensitive formula firming and wrinkle-proofing powder/firming and wrinkle-proofing water. The invention creatively combines the anti-inflammatory and anti-allergy soothing components with other anti-inflammatory and anti-allergy soothing components in a specific ratio, and can synergistically improve the effects of anti-inflammation, anti-allergy and analgesia.

In a preferred embodiment of the invention, the content of polysaccharides in the cactus extract is more than or equal to 70 wt%;

the content of the avenanthramides in the oat bran extract is more than or equal to 95 wt%;

the content of matrine in the sophora flavescens extract is more than or equal to 95 wt%;

the content of polysaccharide in the radix ophiopogonis extract is more than or equal to 70 wt%;

the content of paeoniflorin in the radix Paeoniae extract is not less than 60 wt%;

the content of baicalin in the radix Scutellariae extract is not less than 90 wt%;

the content of the albizim in the duck and quillaja saponaria extract is more than or equal to 50 wt%.

In another preferred embodiment of the present invention, the composition comprises only a solvent in addition to the active ingredient.

In a preferred embodiment of the present invention, the solvent is a mixed solvent of water, butanediol, and pentanediol.

In another aspect, the present invention relates to a method for preparing the above composition, comprising the steps of: 1) preparing a sophora flavescens extract solution; 2) homogenizing radix et caulis Opuntiae Dillenii extract, oat bran extract, radix Ophiopogonis extract, radix Paeoniae extract, Scutellariae radix extract, fructus Quillaja Saponicae extract, butanediol and pentanediol under ultrahigh pressure to obtain plant extract mixed solution; 3) mixing the plant extract mixed solution and the sophora flavescens extract solution, homogenizing under ultrahigh pressure and filtering.

In another aspect, the invention relates to the use of the above composition for the preparation of a cosmetic product for anti-inflammatory, anti-allergy and/or analgesic use.

In a preferred embodiment of the present invention, the anti-inflammatory and soothing repair composition is added to the skin care product in an amount of 0.5 to 5 wt%.

In a preferred embodiment of the present invention, the skin care product is one of a mask, a skin cream, a face cleanser, a lotion, a skin lotion, a sunscreen cream, a BB cream, a shampoo, a body wash, and a skin care gel.

Advantageous effects

The anti-inflammatory and allergy-relieving repair composition disclosed by the invention is matched with the Quillaja saponaria Molina extract and other plant extracts, so that the anti-inflammatory, anti-allergy and analgesic effects can be improved, the skin barrier function can be enhanced, the self-protection capability of the skin can be improved, the inflammatory skin, especially the skin with hormone-dependent dermatitis can be effectively repaired, and the drug resistance, the dependence and the side effects cannot be generated after long-term use.

Drawings

Fig. 1 is a graph showing the results of skin itch test of guinea pigs caused by histamine phosphate in examples and comparative examples.

FIG. 2 shows the results of the hyaluronidase inhibition test in vitro in examples and comparative examples.

Fig. 3 shows the results of the skin irritation repairing effect test of examples and comparative examples.

FIG. 4 shows the results of the anti-complement effect test in examples and comparative examples.

FIG. 5 shows the results of the test for suppressing the inflammation induced by carrageenan in the examples and comparative examples.

FIG. 6 shows the results of the test for inhibiting inflammation induced by arachidonic acid in examples and comparative examples.

Detailed Description

In order to further understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Unless otherwise specified, the reagents involved in the examples of the present invention are all commercially available products, and all of them are commercially available.

Examples 1 to 3 and comparative examples 1 to 2:

examples 1-3 and comparative examples 1-2 each relate to an anti-inflammatory and soothing rejuvenating composition, the raw material composition of which is shown in table 1 below.

TABLE 1 anti-inflammatory and soothing conditioner composition formula (wt%)

Raw materials	Example 1	Example 2	Example 3	Comparative example 1	Comparative example 2
						Cactus extract (polysaccharide is more than or equal to 70 wt%)	4	4	4	4	-
Oat bran extract (oat alkaloid is more than or equal to 95 wt%)	4	4	4	4	-
						Radix Sophorae Flavescentis extract (matrine is greater than or equal to 95 wt%)	4	4	4	4	-
Radix Ophiopogonis extract (polysaccharide ≥ 70 wt%)	4	4	4	4	-
						Radix Paeoniae extract (paeoniflorin ≥ 60 wt%)	4	4	4	4	-
Radix Scutellariae extract (baicalin greater than 90 wt%)	4	4	4	4	-
						Quillaja extract (with farnesin content not less than 50 wt%)	2	1	3	-	4
Butanediol	40	40	40	40	40
						Pentanediol	9	9	9	9	9
Water (W)	Balance of	Balance of	Balance of	Balance of	Balance of

The preparation process of examples 1-3 comprises the following steps:

1) mixing radix Sophorae Flavescentis extract and water thoroughly to obtain radix Sophorae Flavescentis extract solution;

2) mixing radix et caulis Opuntiae Dillenii extract, oat bran extract, radix Ophiopogonis extract, radix Paeoniae extract, Scutellariae radix extract, commercially available duck Quillaja saponaria extract, butanediol and pentanediol, and homogenizing in a high-pressure micro-jet homogenizer (operation pressure of 1200bar) to obtain plant extract mixed solution;

3) adding the plant extract mixed liquor obtained in the step 2) into a stirrer, starting stirring, heating to 50 ℃, adding the sophora flavescens extract solution obtained in the step 1), fully mixing, transferring into a high-pressure micro-jet homogenizer (operating pressure 1500bar), circularly homogenizing for 3 times, and filtering by using an ultra-micro filter membrane with the aperture of 100nm to obtain the anti-inflammatory and allergy-relieving repairing composition.

Comparative example 1 was prepared similarly to examples 1-3, except that no duck quillaja extract was added in step 2).

The preparation method of comparative example 2 is to fully and uniformly mix the duck quillaja extract, butanediol and pentanediol, and then to transfer the mixture into a high-pressure micro-jet homogenizer (with the operation pressure of 1200bar) for homogenization to obtain a mixed solution; adding the mixed solution into a stirrer, stirring, heating to 50 deg.C, transferring into a high-pressure microjet homogenizer (operating pressure 1500bar), homogenizing for 3 times, and filtering with ultramicro filter membrane with aperture of 100nm to obtain the antiinflammatory and allergy-relieving repairing composition.

Pharmacodynamic experiment

1. Skin itch test of guinea pigs caused by histamine phosphate:

100 guinea pigs, each of which had both males and females, were randomly divided into 5 groups, the backs of the right hind feet of the guinea pigs were shaved, 0.5g of the anti-inflammatory and soothing treatment composition of examples 1-3 and comparative examples 1-2, each of which had a total active ingredient content of 80mg/kg, was applied to each shaved area, and after 1 hour, the skin at the shaved area was gently abraded with coarse sandpaper, and the area of the abraded area was about 1cm2, and the abraded area was further applied with the above-mentioned groups and doses of histamine phosphate, in which the concentration was gradually increased (0.01%, 0.02%, 0.03%, 0.04%, and 0.05% …) at intervals of 3 minutes after 10 minutes, 0.05 mL/mouse per time until the guinea pigs licked back the right hind feet, and the total amount of histamine phosphate administered when the right hind feet of the guinea pigs finally licked, was regarded as the scratchiness threshold, as shown in fig. 1.

As can be seen from fig. 1: the anti-inflammatory and allergy-relieving repair compositions of examples 1 to 3 and comparative examples 1 to 2 all have a certain inhibitory effect on skin itch of guinea pigs caused by histamine phosphate, wherein the anti-inflammatory and allergy-relieving repair compositions of examples 1 to 3 and comparative examples 1 to 2 all have a significant inhibitory effect on itch reactions of guinea pigs caused by histamine phosphate, while the anti-inflammatory and allergy-relieving repair composition of example 1 has the strongest inhibitory effect and the best anti-allergy and itching relieving effect.

2. Hyaluronidase in vitro inhibition assay:

1) the test groups were divided into 4 groups of A, B, C, D, 0.5mL of hyaluronidase (500U/mL) was added to each of group A and group C, 0.5mL of acetate buffer (pH 5.6) was added to each of group B and group D, and 0.1mL of CaCl was added thereto₂Placing the solution (2.5mol/L) in a constant-temperature water bath kettle at 37 ℃ for heat preservation for 20 min;

2) taking out group A and group B, respectively adding into the sample solution, respectively adding distilled water into group C and group D, and then keeping the temperature in a constant-temperature water bath kettle at 37 ℃ for 20 min;

3) taking out the group A and the group C, respectively adding 0.5mL of sodium hyaluronate solution (0.5mg/mL), respectively adding 0.5mL of acetic acid solution (pH value 5.6) into the group B and the group D, and then preserving the heat in a constant-temperature water bath kettle at 37 ℃ for 30 min;

4) taking out the groups A to D, standing at room temperature for 5min, respectively adding 0.5mL of NaOH solution (0.5mol/L) and 0.5mL of acetylacetone solution (3.5mL of acetylacetone +50mL of sodium carbonate solution with the concentration of 1.0 mol/L) into each group, then boiling in a water bath for 15min, carrying out an ice water bath for 5min, standing at room temperature for 10min, respectively adding 1mL of an Ellisib reagent (0.8g of p-dimethylaldehyde +15mL of concentrated hydrochloric acid +15mL of anhydrous ethanol) into each group, fully oscillating, respectively adding 3mL of anhydrous ethanol for dilution, standing at room temperature for 20min for color development, measuring the absorbance value, and substituting the formula to calculate the inhibition ratio:

hyaluronidase inhibition (%) - (C-D) - (a-B) ]/(C-D) × 100%,

in the formula: a is the absorbance value of the tested sample solution, B is the absorbance value of the reagent blank solution, C is the absorbance value of the control solution, and D is the absorbance value of the blank control solution.

The anti-inflammatory and soothing repairing compositions of examples 1-3 and comparative examples 1-2, all of which have a total active content of 6mg/mL, were tested for their in vitro inhibition rate of hyaluronidase by the above test method, and the test results are shown in fig. 2.

As can be seen from fig. 2: the anti-inflammatory and soothing repairing compositions of examples 1 to 3 and comparative examples 1 to 2 all had the effect of inhibiting the activity of hyaluronidase, while the anti-inflammatory and soothing repairing composition of example 1 had the effect of inhibiting hyaluronidase by more than 95% at an active ingredient of 6mg/mL, which was better than the anti-inflammatory and soothing repairing compositions of comparative examples 1 to 2.

3. Skin irritation repair effect test:

1) 10 volunteers are collected, the sex is random, the inner sides of the left and right arms of the volunteers are taken as tested parts, and a square area of 2cm multiplied by 2cm is selected as a tested area;

2) cleaning a tested area with clear water, standing for 10min, measuring a skin red value by using a German CK multifunctional skin tester, stimulating the tested area by using a capsaicin aqueous solution (about 0.01mL) with the mass fraction of 50%, and collecting data after continuously stimulating for 15min and 30 min;

3) smearing 0.05mL of test sample (total active matter content is 6mg/mL) on a tested area, collecting data after 15min, 30min and 45min, smearing 0.05mL of pure water on a blank control, counting the value measured at each test part of a volunteer, and analyzing the change of red value.

The skin red value change reflects the skin red time change rule of the tested area in the test period, and the larger the value is, the larger the skin red degree is.

Skin redness change rate (%) - (T-T)_{Blank value})/T_{Blank value}×100％。

Using the above test methods, the anti-inflammatory and soothing skin care compositions of examples 1-3 and comparative examples 1-2, all having a total active content of 6mg/mL, were tested for skin irritation and the results are shown in FIG. 3.

As can be seen from fig. 3, the anti-inflammatory and soothing repair compositions of examples 1 to 3 and comparative examples 1 to 2 all had a certain degree of repair efficacy on skin after stimulation with an aqueous capsaicin solution, and the anti-inflammatory and soothing repair compositions of examples 1 to 3 had a more significant repair effect on skin after stimulation and were able to repair damaged skin quickly, compared to the anti-inflammatory and soothing repair compositions of comparative examples 1 to 2.

4. Anti-complement action test:

the test principle is as follows: the complement system can be activated by antigen-antibody complexes, causing a hemolytic reaction that leads to the escape of hemoglobin; the inhibition rate of the test sample on the complement action can be judged according to the height of the absorbance value by measuring the absorbance value of the hemoglobin. The formula for calculating the inhibition rate is as follows: inhibition ratio (%) - (A-A)_{Blank space})/A_{Total hemolysis}×100％。

Injecting the antigen-antibody complex and the test sample into the sheep blood erythrocyte suspension, setting full hemolysis and blank control, incubating for 30min at 32 ℃, continuously shaking, centrifuging for 10min, taking the supernatant, and measuring the absorbance value of the supernatant by using a spectrophotometer (560 nm). The anti-complement activation effect was evaluated by the inhibition rate of the extract on hemolysis.

The anti-inflammatory and soothing repair compositions of examples 1-3 and comparative examples 1-2 (total active content was 6mg/g) were tested for anti-complement effect using the method described above and the results are shown in figure 4.

As can be seen from fig. 4: the anti-inflammatory and soothing repair compositions of examples 1 to 3 almost completely inhibited the anti-complement effect and were excellent compared to the anti-inflammatory and soothing repair compositions of comparative examples 1 to 2.

5. Test for inhibition of carrageenan-induced inflammation:

the thickness of the soles of ICR female mice was measured for 6 weeks, 25mg of the test sample (total active content was 6mg/g) was applied to the right soles, 25mg of physiological saline was applied to the left soles, 1 time was applied after 24 hours, 1 time was applied after 1 hour, 25. mu.L of physiological saline containing 2% of lambda-carrageenan was applied to both soles of mice after 1 hour, the thickness of both soles was measured once per hour for 4 hours, and the difference between before and after application of the test sample was measured and compared with a blank control group to calculate the inhibition rate.

Edema inhibition (%) - (Z-Z)_{Right initial value})/(Z_{Blank value}-Z_{Left initial value})×100％。

The anti-inflammatory and soothing repair compositions of examples 1-3 and comparative examples 1-2 were tested for their effect on inhibition of carrageenan-induced inflammation, and the results are shown in figure 5.

As can be seen from fig. 5: the anti-inflammatory and soothing composition of examples 1-3 had a very good effect of suppressing the carrageenan-induced inflammation and was more effective over time than the anti-inflammatory and soothing composition of comparative examples 1-2.

6. Test for inhibition of arachidonic acid induced inflammation:

1) dividing 30 guinea pigs into 5 groups, smearing mixture of p-phenylenediamine and olive oil (the mass ratio of p-phenylenediamine to olive oil is 4:1) on ears of two sides of guinea pigs, and smearing 0.1mL per day for 3 days;

2) after 5 days, 15mg of the test specimen was applied to one ear 3 times, 1 hour after the last application was wiped off, and 20. mu.L of a p-phenylenediamine-olive oil mixture (p-phenylenediamine, olive oil mass ratio: 4:1) was applied, 17 hours after the application of the test specimen twice, 19 hours after the wiping off of the test specimen, 24 hours after the cutting of 5mm diameter portions of the ears by a punch, respectively, the difference in weight between the ears using the test specimen and the ears not using the test specimen was measured, and the inhibition ratio was calculated by comparison with the reference specimen.

The anti-inflammatory and soothing conditioner compositions of examples 1 to 3 and comparative examples 1 to 2 were mixed with hydrophilic petrolatum, respectively, to prepare test samples having a total active content of 6 wt%, and then tested according to the above method, and the test results are shown in fig. 6.

As can be seen from fig. 6: the use of the anti-inflammatory and soothing relief composition of examples 1-3 before arachidonic acid induces inflammation inhibited edema by more than 60% with better results than comparative examples 1-2.

The foregoing describes preferred embodiments of the present invention, but is not intended to limit the invention thereto. Modifications and variations of the embodiments disclosed herein may be made by those skilled in the art without departing from the scope and spirit of the invention.

Claims

1. An anti-inflammatory, allergy-relieving, remedial composition comprising the following active ingredients:

cactus extract;

oat bran extract;

radix Sophorae Flavescentis extract;

an extract of the root of Ophiopogon japonicus;

radix Paeoniae extract;

scutellariae radix extract;

extract of Quillaja saponaria Molina.

2. The composition according to claim 1, wherein the active ingredients comprise the following components in percentage by mass:

cactus extract: 1% -15%;

oat bran extract: 1% -5%;

and (3) sophora flavescens extract: 1% -5%;

radix ophiopogonis extract: 1% -5%;

peony root extract: 1% -5%;

radix scutellariae extract: 1% -5%;

duck Quillaja saponaria extract: 1 to 3 percent.

3. The composition according to claim 1 or 2, wherein the weight percentage of the duck quillaja extract is 1.5-2.5%.

4. The composition according to claim 1, wherein the active ingredients comprise the following components in percentage by mass:

cactus extract: 2% -4%;

oat bran extract: 2% -4%;

and (3) sophora flavescens extract: 2% -4%;

radix ophiopogonis extract: 2% -4%;

peony root extract: 2% -4%;

radix scutellariae extract: 2% -4%;

duck Quillaja saponaria extract: 1.8 to 2.2 percent.

5. The composition according to claim 1, wherein the content of polysaccharides in the cactus extract is more than or equal to 70 wt%;

6. The composition of claim 1, comprising only a solvent in addition to the active ingredient.

7. The composition of claim 6, wherein the solvent is a mixed solvent of water, butanediol, and pentanediol.

8. Use of a composition according to any one of claims 1 to 7 for the preparation of a cosmetic product for anti-inflammatory, anti-allergy and/or analgesic use.

9. The use according to claim 8, wherein the anti-inflammatory, soothing, and repairing composition is added to the skin care product in an amount of 0.5 to 5 wt%.

10. The use of claim 8, wherein the skin care product is one of a facial mask, a skin cream, a face cleanser, a lotion, a skin lotion, a sunscreen cream, a BB cream, a shampoo, a body wash, and a skin care gel.