CN114096661B - 改进了安全性及抗肿瘤效果的溶瘤病毒 - Google Patents

改进了安全性及抗肿瘤效果的溶瘤病毒 Download PDF

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CN114096661B
CN114096661B CN202080047363.4A CN202080047363A CN114096661B CN 114096661 B CN114096661 B CN 114096661B CN 202080047363 A CN202080047363 A CN 202080047363A CN 114096661 B CN114096661 B CN 114096661B
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virus
arg
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CN114096661A (zh
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黄泰皓
赵蒙
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Bayer Knox Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/16—Amides, e.g. hydroxamic acids
    • A61K31/17—Amides, e.g. hydroxamic acids having the group >N—C(O)—N< or >N—C(S)—N<, e.g. urea, thiourea, carmustine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A61K31/52—Purines, e.g. adenine
    • A61K31/522—Purines, e.g. adenine having oxo groups directly attached to the heterocyclic ring, e.g. hypoxanthine, guanine, acyclovir
    • A—HUMAN NECESSITIES
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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN202080047363.4A 2019-06-27 2020-06-29 改进了安全性及抗肿瘤效果的溶瘤病毒 Active CN114096661B (zh)

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KR10-2019-0077377 2019-06-27
KR20190077377 2019-06-27
PCT/KR2020/008472 WO2020263059A1 (ko) 2019-06-27 2020-06-29 안전성 및 항암효과가 개선된 항암바이러스

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EP (1) EP3992281A4 (enExample)
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CN (1) CN114096661B (enExample)
AU (1) AU2020306544B2 (enExample)
CA (1) CA3139643A1 (enExample)
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CN120037270A (zh) * 2019-08-26 2025-05-27 拜耳诺克斯有限公司 包含痘苗病毒和羟基脲作为活性成分的用于治疗癌症的药物组合物

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN111556899A (zh) * 2017-12-28 2020-08-18 拜耳诺克斯有限公司 安全性和抗癌作用得到改善的溶瘤病毒

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2699191B1 (fr) * 1992-12-16 1995-02-10 Univ Paris Curie Nouveaux vecteurs rétroviraux, lignées cellulaires les contenant, et leur utilisation dans le traitement des tumeurs.
BR9707483A (pt) 1996-02-09 1999-04-06 Rhone Poulenc Rorer Sa Sequência de ácidos nucléicos que codificam para uma timidina quinase variante de uma timidina quinase selvagem variante de uma timidina quinase do vírus do herpes simplex de tipo 1 veículo de expressão vetor e composição farmacêutica
GB0008966D0 (en) * 2000-04-13 2000-05-31 Imp College Innovations Ltd Vectors for gene therapy
WO2002042468A2 (en) * 2000-11-27 2002-05-30 Geron Corporation Glycosyltransferase vectors for treating cancer
US20040053836A1 (en) 2002-04-22 2004-03-18 Philipp Mayer-Kuckuk Method for modulating the production of a selected protein in vivo
GB0413702D0 (en) * 2004-06-18 2004-07-21 Molmed Spa Thymidine kinase
CN105407902A (zh) * 2013-03-05 2016-03-16 贝勒医学院 溶瘤病毒
EP2967078B1 (en) 2013-03-14 2018-10-17 GenVivo, Inc. Thymidine kinase diagnostic assay for gene therapy applications
KR20190059421A (ko) * 2017-11-23 2019-05-31 주식회사 바이오녹스 항암바이러스의 간동맥 투여를 이용한 간암 치료방법
WO2019108777A1 (en) * 2017-11-29 2019-06-06 Research Development Foundation Elimination of proliferating cells from stem cell-derived grafts
CN114630683A (zh) * 2019-08-29 2022-06-14 拜耳诺克斯有限公司 包含痘苗病毒和粒细胞生成抑制剂作为活性成分的用于治疗癌症的药物组合物

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111556899A (zh) * 2017-12-28 2020-08-18 拜耳诺克斯有限公司 安全性和抗癌作用得到改善的溶瘤病毒

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CA3139643A1 (en) 2020-12-30
EP3992281A1 (en) 2022-05-04
AU2020306544B2 (en) 2025-10-16
EP3992281A4 (en) 2023-07-26
JP7704419B2 (ja) 2025-07-08
AU2020306544A1 (en) 2021-12-02
WO2020263059A1 (ko) 2020-12-30
US20220202885A1 (en) 2022-06-30
KR102625842B1 (ko) 2024-01-17
CN114096661A (zh) 2022-02-25
JP2022539016A (ja) 2022-09-07
KR20210002056A (ko) 2021-01-06

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