CN114041457A - Method for preparing green plant soaked specimen - Google Patents
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- CN114041457A CN114041457A CN202111193444.8A CN202111193444A CN114041457A CN 114041457 A CN114041457 A CN 114041457A CN 202111193444 A CN202111193444 A CN 202111193444A CN 114041457 A CN114041457 A CN 114041457A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/38—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for botany
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Abstract
The invention belongs to the technical field of specimen preparation, and discloses a method for preparing green plant soaked specimens, which comprises the steps of picking; preparing a target body; preparing a specimen biocidal stationary liquid, and soaking the sterilized specimen in the specimen biocidal stationary liquid; taking out the label body after soaking, repeatedly cleaning the specimen body by using distilled water, and wiping off surface moisture by using filter paper; carrying out secondary trimming on the target body in an aseptic environment to obtain a target body to be stored; putting a specimen to be preserved into a specimen bottle filled with specimen preservation solution; and (3) covering a bottle cap of the specimen bottle, sealing the bottle body by using wax, labeling the bottle body of the specimen bottle, and storing the specimen bottle in a dark place at the temperature of 20-25 ℃. The manufacturing method of the green plant infusion specimen can keep the integrity of the plant and the quality of the manufactured specimen is good.
Description
Technical Field
The invention belongs to the technical field of specimen preparation, and particularly relates to a method for preparing green plant soaked specimens.
Background
At present: the plant specimen is a preservation form of plant living bodies, can be directly observed at any time, can be immediately preserved while knowing the shape and the color of the plant, is convenient for future observation, research and collection, and is widely applied to medicine collection, teaching and scientific research. The effect of soaking the specimen by the plant is best, the original characteristics of the plant can be reflected best, and the primary color of the plant can be preserved.
The traditional preparation method of the plant infusion specimen generally comprises the steps of pretreatment, disinfection, fixation and preservation. The traditional plant soaking specimen is firstly trimmed and finished, then disinfectant such as ethanol is directly sprayed on the plant, and the plant is washed and soaked by distilled water to realize disinfection, so that the damage to the plant is large; and the plant is not fixed by the biocidal primary color, the color can be changed, the plant is not beneficial to viewing, and the requirements in scientific research, teaching and practical application can not be met.
Through the above analysis, the problems and defects of the prior art are as follows: the disinfection method in the existing manufacturing method of the plant soaking specimen has great damage to the plant; and the plant is not fixed by the biocidal primary color, the color can be changed, and the requirements in scientific research, teaching and practical application can not be met.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for manufacturing a green plant infusion specimen.
The invention is realized in such a way that the method for manufacturing the green plant soaked specimen comprises the following steps:
step one, determining a picking part, picking a fresh target body from a green plant body, and cleaning the picked fresh target body by using distilled water to remove impurities on the surface of a specimen;
secondly, carrying out primary trimming on the cleaned specimen body, and soaking the trimmed specimen body in a sterilizing disinfectant to kill microorganisms, ova and larvae attached to the specimen body to obtain a sterilized specimen body;
step three, preparing a specimen biocidal stationary liquid, and soaking the sterilized specimen in the specimen biocidal stationary liquid;
the preparation of the biocidal fixing liquid for the specimen comprises the following steps:
(1) adding 50-100ml of formaldehyde into 100-150ml of purified water, and fully dissolving to obtain a formaldehyde solution;
(2) adding a saturated copper sulfate solution into the formaldehyde solution, and stirring at a constant speed in the adding process to obtain a formaldehyde-saturated copper sulfate solution;
(3) adding 70-80% ethanol solution by volume fraction into formaldehyde-saturated copper sulfate solution, and adding 8-12% sodium chloride aqueous solution by volume fraction while stirring to obtain mixed solution;
(4) mixing the mixed solution with a boric acid solution to obtain a specimen biocidal stationary liquid;
taking out the label body after soaking, repeatedly cleaning the specimen body by using distilled water, and wiping off surface moisture by using filter paper; performing secondary trimming on the label body by using tweezers, knives and scissors in a sterile environment to obtain the label body to be stored;
putting the specimen to be preserved into a specimen bottle containing specimen preservation liquid, and adjusting the specimen plants in the specimen bottle by using tweezers;
and step six, covering a bottle cap of the sample bottle, sealing the bottle body by using wax, labeling the bottle body of the sample bottle, and storing the sample bottle in a dark place at the temperature of 20-25 ℃.
Further, the preparation method of the sterilizing disinfectant comprises the following steps:
1) putting pine in a drying oven for full drying, and crushing the dried pine to obtain pine powder; mixing pine wood powder with water, extracting with water for 2-3 hr, and filtering to obtain pine wood primary extract;
2) mixing the pine primary extract with 75% ethanol solution by volume, and performing reflux extraction to obtain pine extract;
3) grinding green tea to obtain green tea powder, mixing the green tea powder with ethyl acetate, adding distilled water, and performing continuous countercurrent extraction to obtain green tea extractive solution;
4) soaking herba Elephantopi scaberis in water, and heating the soaking solution together with herba Elephantopi scaberis until the color of the soaking solution changes to obtain herba Elephantopi scaberis extractive solution;
5) mixing the pine extract, the green tea extract and the elephantopus scaber extract in proportion to obtain the sterilizing disinfectant.
Further, the mixing of the pine extract, the green tea extract and the elephantopus scaber extract according to the proportion comprises the following steps: mixing the pine extract 6-8 parts, the green tea extract 2-4 parts and the elephantopus scaber extract 1-3 parts by weight.
Further, in the third step, the sterilized specimen is placed in the specimen biocidal stationary liquid for soaking, the soaking time is determined according to the type of the specimen, the soaking time of the leaf specimen is 10-12 days, and the soaking time of the non-leaf specimen is 15-18 days.
Further, in the fourth step, the secondary trimming of the label body is performed by using tweezers, knives and scissors, and the method comprises the following steps: trimming along the texture and vein of the target body.
Further, in the fifth step, the specimen preservation solution is composed of, by mass, 20-30 parts of purified water, 3-5 parts of a sulfurous acid solution, and 1-2 parts of glycerol.
Further, the preparation method of the specimen preservation solution comprises the following steps:
1) mixing purified water with a sulfurous acid solution to prepare a sulfurous acid solution with the volume fraction of 0.1-0.3%;
2) mixing purified water with glycerol to prepare 0.4-0.6% glycerol solution;
3) mixing 0.1-0.3% by volume of sulfurous acid solution and 0.4-0.6% by volume of glycerol solution, adding purified water, and stirring to obtain specimen preserving fluid.
Further, in the fifth step, the adjusting the specimen plant body in the specimen bottle using the forceps includes: the specimen plant body faces the transparent part of the specimen bottle.
Further, in step five, the tag includes: date of specimen collection, date of preparation, and specimen name.
Another object of the present invention is to provide a green plant infusion specimen prepared by the method for preparing a green plant infusion specimen.
By combining all the technical schemes, the invention has the advantages and positive effects that:
in the manufacturing method, the plants are disinfected, the disinfectant is natural and reliable, the specimen body is not damaged while the sterilization and disinfection are realized, the integrity of the plants can be kept, and the manufactured specimen has better quality; the used components in the fixing solution and the preservation solution can be soaked to better fix the primary colors of the plants; the plant soaked specimen prepared by the preparation method has long color maintenance time and long preservation time, is not easy to rot and deteriorate, and is beneficial to long-term preservation of plants.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 shows a method for preparing a green plant infusion specimen according to an embodiment of the present invention.
Fig. 2 is a flowchart of a method for preparing a target body according to an embodiment of the present invention.
Fig. 3 is a flowchart of a method for preparing a sterilizing liquid according to an embodiment of the present invention.
Fig. 4 is a flow chart for performing the formulation of a specimen biocidal fixative as provided by an embodiment of the present invention.
FIG. 5 is a flowchart of a method for preparing a specimen preservation solution according to an embodiment of the present invention.
FIG. 6 is a graph 1 showing the effect of the green plant infusion specimen provided by the embodiment of the present invention.
FIG. 7 is a graph 2 showing the effect of the green plant infusion specimen provided by the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a method for manufacturing a green plant infusion specimen, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the method for preparing a green plant infusion specimen according to the embodiment of the present invention includes:
s101, preparing a target body; preparing a specimen biocidal stationary liquid, and soaking the sterilized specimen in the specimen biocidal stationary liquid;
s102, taking out the label body after soaking, repeatedly cleaning the specimen body by using distilled water, and wiping off surface moisture by using filter paper; performing secondary trimming on the label body by using tweezers, knives and scissors in a sterile environment to obtain the label body to be stored;
s103, placing the specimen to be preserved into a specimen bottle containing specimen preservation liquid, and adjusting the specimen plants in the specimen bottle by using tweezers;
s104, covering a bottle cap of the specimen bottle, sealing the bottle body with wax, labeling the bottle body of the specimen bottle, and storing the specimen bottle in a dark place at the temperature of 20-25 ℃.
As shown in fig. 2, the preparation of the specimen body according to the embodiment of the present invention includes:
s201, determining a picking part, picking a fresh target body from a green plant body, cleaning the picked fresh target body by using distilled water, and removing impurities on the surface of a specimen;
s202, carrying out primary trimming on the cleaned specimen, and soaking the trimmed specimen in a sterilizing disinfectant to kill microorganisms, ova and larvae attached to the specimen to obtain the sterilized specimen.
As shown in fig. 3, a method for preparing a sterilizing liquid provided by an embodiment of the present invention includes:
s301, putting pine in a drying oven for full drying, and crushing the dried pine to obtain pine powder; mixing pine wood powder with water, extracting with water for 2-3 hr, and filtering to obtain pine wood primary extract;
s302, mixing the pine primary extract with 75% ethanol solution by volume fraction, and performing reflux extraction to obtain a pine extract;
s303, grinding green tea to obtain green tea powder, mixing the green tea powder with ethyl acetate, and adding distilled water to perform continuous countercurrent extraction to obtain a green tea extracting solution;
s304, soaking the elephantopus scaber in water, and heating the soaking solution and the elephantopus scaber until the color of the soaking solution changes to obtain an elephantopus scaber extracting solution;
s305, mixing the pine extract, the green tea extract and the elephantopus scaber extract in proportion to obtain the sterilizing disinfectant.
The embodiment of the invention provides a method for mixing pine extract, green tea extract and elephantopus scaber extract according to the proportion, which comprises the following steps: the tea is composed of 6-8 parts of pine extract, 2-4 parts of green tea extract and 1-3 parts of elephantopus scaber extract in parts by mass.
As shown in fig. 4, the preparation of the biocidal fixing solution for the specimen provided by the embodiment of the present invention includes:
s401, adding 50-100ml of formaldehyde into 100-150ml of purified water, and fully dissolving to obtain a formaldehyde solution;
s402, adding a saturated copper sulfate solution into the formaldehyde solution, and stirring at a constant speed in the adding process to obtain a formaldehyde-saturated copper sulfate solution;
s403, adding 70-80% by volume of ethanol solution into the formaldehyde-saturated copper sulfate solution, and adding 8-12% by volume of sodium chloride aqueous solution while stirring to obtain a mixed solution;
and S404, mixing the mixed solution with a boric acid solution to obtain the biocidal stationary liquid for the specimen.
The sterilized specimen body is placed in the specimen biocidal stationary liquid for soaking, the soaking time is determined according to the type of the specimen, the soaking time of the leaf specimen is 10-12 days, and the soaking time of the non-leaf specimen is 15-18 days.
The secondary trimming of the label body by using the tweezers, the knives and the scissors provided by the embodiment of the invention comprises the following steps: trimming along the texture and vein of the target body.
The specimen preservation solution provided by the embodiment of the invention comprises, by mass, 20-30 parts of purified water, 3-5 parts of a sulfurous acid solution and 1-2 parts of glycerol.
As shown in fig. 5, a method for preparing a specimen preservation solution according to an embodiment of the present invention includes:
s501, mixing purified water with a sulfurous acid solution to prepare a sulfurous acid solution with the volume fraction of 0.1-0.3%;
s502, mixing purified water and glycerol to prepare a glycerol solution with the volume fraction of 0.4-0.6%;
s503, mixing 0.1-0.3% by volume of a sulfurous acid solution and 0.4-0.6% by volume of a glycerol solution, adding purified water, and uniformly stirring to obtain a specimen preservation solution.
The method for adjusting the specimen plant in the specimen bottle by using the tweezers provided by the embodiment of the invention comprises the following steps: the specimen plant body faces the transparent part of the specimen bottle.
The label provided by the embodiment of the invention comprises: date of specimen collection, date of preparation, and specimen name.
The technical effects of the present invention will be further explained below with reference to specific experiments.
Example 1
A method for manufacturing a green plant infusion specimen comprises the following steps:
step one, determining a picking part, picking a fresh target body from a green plant body, and cleaning the picked fresh target body by using distilled water to remove impurities on the surface of a specimen;
secondly, carrying out primary trimming on the cleaned specimen body, and soaking the trimmed specimen body in a sterilizing disinfectant to kill microorganisms, ova and larvae attached to the specimen body to obtain a sterilized specimen body;
step three, preparing a specimen biocidal stationary liquid, and soaking the sterilized specimen in the specimen biocidal stationary liquid;
taking out the label body after soaking, repeatedly cleaning the specimen body by using distilled water, and wiping off surface moisture by using filter paper; performing secondary trimming on the label body by using tweezers, knives and scissors in a sterile environment to obtain the label body to be stored;
putting the specimen to be preserved into a specimen bottle containing specimen preservation liquid, and adjusting the specimen plants in the specimen bottle by using tweezers;
and step six, covering a bottle cap of the sample bottle, sealing the bottle body by using wax, labeling the bottle body of the sample bottle, and storing the sample bottle in a dark place at the temperature of 20 ℃.
In the second step, the preparation method of the sterilizing disinfectant comprises the following steps:
1) putting pine in a drying oven for full drying, and crushing the dried pine to obtain pine powder; mixing pine wood powder with water, extracting with water for 2.5 hr, and filtering to obtain pine wood primary extract;
2) mixing the pine primary extract with 75% ethanol solution by volume, and performing reflux extraction to obtain pine extract;
3) grinding green tea to obtain green tea powder, mixing the green tea powder with ethyl acetate, adding distilled water, and performing continuous countercurrent extraction to obtain green tea extractive solution;
4) soaking herba Elephantopi scaberis in water, and heating the soaking solution together with herba Elephantopi scaberis until the color of the soaking solution changes to obtain herba Elephantopi scaberis extractive solution;
5) mixing the pine extract, the green tea extract and the elephantopus scaber extract in proportion to obtain a sterilizing disinfectant;
in the second step, the mixing of the pine extract, the green tea extract and the elephantopus scaber extract according to the proportion comprises the following steps: mixing 7 parts of pine extract, 3 parts of green tea extract and 2 parts of elephantopus scaber extract in parts by mass.
And in the third step, the sterilized specimen is placed in a specimen biocidal stationary liquid for soaking, the soaking time is determined according to the type of the specimen, the soaking time of the leaf specimen is 11 days, and the soaking time of the non-leaf specimen is 15 days.
The preparation of the biocidal fixing liquid for the specimen comprises the following steps:
(1) adding 50ml of formaldehyde into 120ml of purified water, and fully dissolving to obtain a formaldehyde solution;
(2) adding a saturated copper sulfate solution into the formaldehyde solution, and stirring at a constant speed in the adding process to obtain a formaldehyde-saturated copper sulfate solution;
(3) adding 75% ethanol solution in volume fraction into formaldehyde-saturated copper sulfate solution, and adding 10% sodium chloride aqueous solution in volume fraction while stirring to obtain mixed solution;
(4) and (4) mixing the mixed solution obtained in the step (3) with a boric acid solution to obtain the biocidal stationary liquid for the specimen.
Use tweezers, sword, cut and carry out the secondary pruning of mark body, include: trimming along the texture and vein of the target body.
The specimen preservation solution consists of 25 parts by mass of purified water, 4 parts by mass of sulfurous acid solution and 1.5 parts by mass of glycerol.
The preparation method of the specimen preservation solution comprises the following steps:
1) mixing purified water with a sulfurous acid solution to prepare a sulfurous acid solution with the volume fraction of 0.2%;
2) mixing purified water and glycerol to prepare a glycerol solution with the volume fraction of 0.5%;
3) mixing a 0.2 volume percent sulfurous acid solution and a 0.5 volume percent glycerol solution, adding purified water, and uniformly stirring to obtain a specimen preservative fluid.
In the fifth step, the adjusting of the specimen plant body in the specimen bottle using the forceps includes: making the specimen plant body face the transparent part of the specimen bottle;
in step six, the label includes: date of specimen collection, date of preparation, and specimen name.
Comparative example 1
In addition to the composition of the disinfectant in example 1, the disinfectant is replaced by the composition comprising 0.1-0.2 parts by weight of plant source compound, 0.81.0 parts by weight of tea tree essential oil, 1.31.5 parts by weight of tea polyphenol, 1.02.0 parts by weight of wormwood oil, 2530 parts by weight of lavender extract, 22.5 parts by weight of sandalwood, 2.53.0 parts by weight of anthocyanin, 1.52.0 parts by weight of rhizoma atractylodis and 5865.9 parts by weight of purified water, and the disinfectant is the same as the disinfectant in example 1.
Comparative example 2
Based on example 1, the biocidal fixing solution for replacing specimen comprises the following specific components of multicolor biocidal fixing solution A liquid 95% alcohol 50r11l, copper acetate 50g, 40% formalin 50ml, sodium chloride 10g, glycerol 50, boric acid 10g, and distilled water to 1000 ml. And B, liquid B: 5% formalin in water. The rest is the same as example 1.
Comparative example 3
Based on the embodiment 1, the composition of the substitute specimen preservation solution comprises 1-5g of potassium sorbate, 0.1-1g of nano-silver, 100ml of ethanol, 1-20ml of glycerol and 10-20g of citric acid, the pH is adjusted to be kept at 5-6, and water is added to the solution to reach the constant volume of 1000 ml. The rest is the same as example 1.
TABLE 1
As can be seen from the data in table 1, the solution of example 1 is superior to comparative example 1, comparative example 2, comparative example 3 and the prior art.
TABLE 2 this table is the comparison of the preservation effect of the treated flower of washing bowls
As is clear from Table 2, the preservation effect of the present invention is superior to that of the prior art. Therefore, compared with the prior art, the technical scheme of the invention has significant progress.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.
Claims (10)
1. The method for manufacturing the green plant infusion specimen is characterized by comprising the following steps of:
step one, determining a picking part, picking a fresh target body from a green plant body, and cleaning the picked fresh target body by using distilled water to remove impurities on the surface of a specimen;
secondly, carrying out primary trimming on the cleaned specimen body, and soaking the trimmed specimen body in a sterilizing disinfectant to kill microorganisms, ova and larvae attached to the specimen body to obtain a sterilized specimen body;
step three, preparing a specimen biocidal stationary liquid, and soaking the sterilized specimen in the specimen biocidal stationary liquid;
taking out the label body after soaking, repeatedly cleaning the specimen body by using distilled water, and wiping off surface moisture by using filter paper; carrying out secondary trimming on the target body in an aseptic environment to obtain a target body to be stored;
putting the specimen to be preserved into a specimen bottle containing specimen preservation liquid, and adjusting the specimen plants in the specimen bottle by using tweezers;
and step six, covering a bottle cap of the specimen bottle, sealing the bottle body, and storing the specimen bottle in a dark place at the temperature of 20-25 ℃.
2. The method for preparing the green plant infusion specimen according to the claim 1, wherein in the second step, the method for preparing the sterilizing disinfectant comprises the following steps:
1) putting pine in a drying oven for full drying, and crushing the dried pine to obtain pine powder; mixing pine wood powder with water, extracting with water for 2-3 hr, and filtering to obtain pine wood primary extract;
2) mixing the pine primary extract with 75% ethanol solution by volume, and performing reflux extraction to obtain pine extract;
3) grinding green tea to obtain green tea powder, mixing the green tea powder with ethyl acetate, adding distilled water, and performing continuous countercurrent extraction to obtain green tea extractive solution;
4) soaking herba Elephantopi scaberis in water, and heating the soaking solution together with herba Elephantopi scaberis until the color of the soaking solution changes to obtain herba Elephantopi scaberis extractive solution;
5) mixing the pine extract, the green tea extract and the elephantopus scaber extract in proportion to obtain the sterilizing disinfectant.
3. The method for preparing the green plant infusion specimen according to the claim 2, wherein the step two, the mixing of the pine extract, the green tea extract and the elephantopus scaber extract according to the proportion comprises: mixing the pine extract 6-8 parts, the green tea extract 2-4 parts and the elephantopus scaber extract 1-3 parts by weight.
4. The method for preparing the green plant infusion specimen according to claim 1, wherein in the second step, the third step, the sterilized specimen is soaked in the specimen biocidal stationary liquid for a soaking time determined according to the type of the specimen, the soaking time of the leaf specimen is 10 to 12 days, and the soaking time of the non-leaf specimen is 15 to 18 days.
5. The method for preparing the green plant infusion specimen according to claim 1, wherein in the third step, the preparation of the specimen biocidal fixing solution comprises the following steps:
(1) adding 50-100ml of formaldehyde into 100-150ml of purified water, and fully dissolving to obtain a formaldehyde solution;
(2) adding a saturated copper sulfate solution into the formaldehyde solution, and stirring at a constant speed in the adding process to obtain a formaldehyde-saturated copper sulfate solution;
(3) adding 70-80% ethanol solution by volume fraction into formaldehyde-saturated copper sulfate solution, and adding 8-12% sodium chloride aqueous solution by volume fraction while stirring to obtain mixed solution;
(4) and (4) mixing the mixed solution obtained in the step (3) with a boric acid solution to obtain the biocidal stationary liquid for the specimen.
6. The method for preparing the green plant infusion specimen according to the claim 1, wherein the step four is that the secondary trimming of the specimen body is performed by using tweezers, knives and scissors, and comprises the following steps: trimming along the texture and vein of the target body.
7. The method for preparing the green plant infusion specimen according to claim 1, wherein in the fifth step, the specimen preservation solution is composed of, by mass, 20-30 parts of purified water, 3-5 parts of a sulfurous acid solution, and 1-2 parts of glycerol.
8. The method for preparing the green plant infusion specimen according to claim 7, wherein in the fifth step, the preparation method of the specimen preservation solution comprises:
1) mixing purified water with a sulfurous acid solution to prepare a sulfurous acid solution with the volume fraction of 0.1-0.3%;
2) mixing purified water with glycerol to prepare 0.4-0.6% glycerol solution;
3) mixing 0.1-0.3% by volume of sulfurous acid solution and 0.4-0.6% by volume of glycerol solution, adding purified water, and stirring to obtain specimen preserving fluid.
9. The method for preparing the green plant infusion specimen according to claim 1, wherein the step five of adjusting the specimen plant body in the specimen bottle using tweezers comprises: making the specimen plant body face the transparent part of the specimen bottle;
in step six, the label includes: date of specimen collection, date of preparation, and specimen name.
10. A green plant infusion specimen prepared by the method of making a green plant infusion specimen of any one of claims 1 to 9.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
| CN113439741A (en) * | 2021-08-05 | 2021-09-28 | 湖北省农业科学院中药材研究所 | Preparation method of primary color soaked specimen of snake mushroom |
-
2021
- 2021-10-13 CN CN202111193444.8A patent/CN114041457A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
| CN102524245A (en) * | 2011-12-15 | 2012-07-04 | 河南科技大学 | Preparation method of plant unbleached impregnated specimen |
| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
| CN113439741A (en) * | 2021-08-05 | 2021-09-28 | 湖北省农业科学院中药材研究所 | Preparation method of primary color soaked specimen of snake mushroom |
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